CN111938230A - Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask - Google Patents
Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask Download PDFInfo
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- CN111938230A CN111938230A CN202010548759.9A CN202010548759A CN111938230A CN 111938230 A CN111938230 A CN 111938230A CN 202010548759 A CN202010548759 A CN 202010548759A CN 111938230 A CN111938230 A CN 111938230A
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- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D13/00—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches
- A41D13/05—Professional, industrial or sporting protective garments, e.g. surgeons' gowns or garments protecting against blows or punches protecting only a particular body part
- A41D13/11—Protective face masks, e.g. for surgical use, or for use in foul atmospheres
-
- A—HUMAN NECESSITIES
- A41—WEARING APPAREL
- A41D—OUTERWEAR; PROTECTIVE GARMENTS; ACCESSORIES
- A41D27/00—Details of garments or of their making
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B5/00—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts
- B32B5/02—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by structural features of a fibrous or filamentary layer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B5/00—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts
- B32B5/22—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed
- B32B5/24—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed one layer being a fibrous or filamentary layer
- B32B5/26—Layered products characterised by the non- homogeneity or physical structure, i.e. comprising a fibrous, filamentary, particulate or foam layer; Layered products characterised by having a layer differing constitutionally or physically in different parts characterised by the presence of two or more layers which are next to each other and are fibrous, filamentary, formed of particles or foamed one layer being a fibrous or filamentary layer another layer next to it also being fibrous or filamentary
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2262/00—Composition or structural features of fibres which form a fibrous or filamentary layer or are present as additives
- B32B2262/08—Animal fibres, e.g. hair, wool, silk
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2307/00—Properties of the layers or laminate
- B32B2307/70—Other properties
- B32B2307/714—Inert, i.e. inert to chemical degradation, corrosion
- B32B2307/7145—Rot proof, resistant to bacteria, mildew, mould, fungi
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2307/00—Properties of the layers or laminate
- B32B2307/70—Other properties
- B32B2307/724—Permeability to gases, adsorption
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2535/00—Medical equipment, e.g. bandage, prostheses, catheter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B32—LAYERED PRODUCTS
- B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- B32B2571/00—Protective equipment
Abstract
The invention discloses a respiratory tract transmitted pathogen enrichment spinning membrane, a sampling mask paster and a sampling mask. The enrichment spinning membrane is composed of a protein spinning membrane, has the characteristics of multi-layer porosity and air permeability, is coupled with and captures an antibody of a respiratory tract transmitted pathogen, can efficiently block external air and the pathogen exhaled from the inside of a human body through the specific combination of the pathogen and the antibody, and realizes bidirectional protection; the sampling mask provided by the invention can capture the pathogens in the breath of a mask wearer while blocking the pathogens, so that the pathogens can be enriched in a patch to the maximum extent, sample collection in a long-distance travel process is facilitated, a complex throat swab process is avoided, doctors and patients are protected, detection pressure is relieved, and effectiveness of subsequent detection and patient investigation efficiency are improved.
Description
Technical Field
The invention relates to the field of biomedical materials, in particular to a respiratory tract transmitted pathogen enrichment spinning film, a sampling mask paster and a sampling mask.
Background
Respiratory infectious diseases refer to diseases caused by invasion of pathogens such as viruses, bacteria, mycoplasma and chlamydia from respiratory infections such as nasal cavity, throat, trachea and bronchus of human body, and include influenza, measles, chicken pox, rubella, epidemic encephalitis, mumps, and pulmonary tuberculosis. The disease is often characterized by strong infectivity, multiple transmission ways, high transmission speed, wide transmission range, common and easy crowd and the like, and becomes one of the most harmful infectious diseases.
Throughout the development history of infectious diseases, the epidemic situation of respiratory infectious diseases is never interrupted, and because of the characteristics of strong infectivity and the like, if the control is improper, the infection is easily caused on a large scale in a short time, the human health is influenced, and even social panic is caused. In order to maintain the steady development of society, the control of respiratory infectious diseases is indispensable.
The infectious chain of respiratory infections mainly consists of three factors: the infection source: primarily patients or occult infected; the transmission path is as follows: including droplet nuclear transmission, aerosol transmission, contact transmission, and the like; susceptible people: the immunity to infection is lacked, and the people are easy to be infected. The destruction of the infection chain is the main control principle of respiratory infectious diseases, and the wearing of a mask to filter air entering a human body to block the transmission path is one of the important measures for preventing the respiratory infectious diseases. The National Institute of Occupational Safety and Health (NIOSH) and the world health organization recommend N95 medical protective masks to be used for preventing and treating respiratory infectious diseases, but most hospitals in China currently mainly wear gauze masks and surgical masks even respiratory infectious disease hospitals, but the masks only have a certain mechanical blocking effect, cannot effectively block pathogens from invading through a respiratory tract, only play a role in simple protection, and still have a certain infection risk.
On the other hand, the nucleic acid detection is a common means for detecting respiratory infectious diseases at present due to the characteristics of high sensitivity, good specificity, good timeliness, strong stability and the like. However, when a throat swab is used for collecting a patient, the throat part needs to be exposed, and exhaled air is likely to be aerosol containing virus and spray, so that the risk of working of throat swab collection staff is extremely high. Meanwhile, one-time sampling requires 3-10 minutes on average, and is easily influenced by human factors, so that the missed diagnosis of positive cases and even serious consequences of spreading of epidemic situation of respiratory infectious diseases are caused.
Disclosure of Invention
The invention aims to solve the technical problems in the prior art and provides a respiratory tract transmitted pathogen enrichment spinning membrane, a sampling mask paster and a sampling mask.
In order to solve the technical problems, the invention adopts the technical scheme that: a respiratory-transmitted pathogen-enriched spun film is provided that includes a protein spun film coupled to a capture antibody that is a specific antibody capable of targeting binding to a plurality of respiratory-transmitted pathogens.
Preferably, the respiratory transmitted pathogens include, but are not limited to, influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, mycobacterium tuberculosis.
Preferably, the protein spinning membrane includes, but is not limited to, a natural plant protein membrane, a plant protein spinning membrane, an animal protein spinning membrane, and an insect spinning membrane.
Preferably, the preparation of the respiratory tract transmitted pathogen enriched spun film comprises the steps of: preparing a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody, and bridging the protein spinning membrane mouse-derived monoclonal antibody and the pathogen specificity mouse-derived monoclonal antibody through an anti-mouse secondary antibody to obtain the respiratory tract transmitted pathogen enriched spinning membrane.
Preferably, the preparation method of the protein spinning membrane comprises the following steps: cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; then evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot pressing plate, and carrying out vertical hot pressing to form the protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
Preferably, the method for preparing the protein-spun membrane murine monoclonal antibody comprises the following steps: immunizing a mouse with the protein spinning membrane antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting a specific anti-protein spinning membrane antigen to obtain a stable hybridoma cell strain; then, the cell is inoculated to the abdominal cavity of a mouse, ascites is prepared, and the specific protein spinning membrane mouse-derived monoclonal antibody is extracted from the ascites.
Preferably, the method for preparing the pathogen-specific murine monoclonal antibody comprises the steps of: immunizing a mouse with a pathogen specific antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting the specific anti-pathogen antigen to obtain a stable hybridoma cell strain; then, the cells are inoculated into the abdominal cavity of a mouse, ascites is prepared, and a specific pathogen-specific murine monoclonal antibody is extracted from the ascites.
Preferably, the preparation method of the anti-mouse secondary antibody comprises the following steps: immunizing goat, horse, donkey, rabbit or chicken with gamma globulin of mouse, immunizing, collecting animal serum, and extracting specific anti-mouse antibody from the serum.
The invention also provides a respiratory tract transmitted pathogen enrichment sampling mask paster which is characterized by being prepared by attaching the single surface of the respiratory tract transmitted pathogen enrichment spinning film to a bonding layer.
The invention also provides a respiratory tract transmitted pathogen enrichment sampling mask which is characterized in that the inner side of the mask is provided with the sampling mask paster.
The invention has the beneficial effects that:
1. the respiratory tract transmitted pathogen enrichment spinning membrane is composed of a protein spinning membrane, has the characteristics of multi-layer porosity and air permeability, is coupled with and captures an antibody of the respiratory tract transmitted pathogen, can efficiently block external air and the pathogen exhaled from the inside of a human body through the specific combination of the pathogen and the antibody, and realizes bidirectional protection;
2. the mask paster can be matched with a traditional mask for use, does not influence the wearing and using mode of the original mask, and can be matched with the mechanical blocking effect of the traditional mask to realize physical and biological dual protection; the mask patch is convenient to use and wide in applicability, and the used materials are healthy and environment-friendly and do not influence normal wearing;
3. the respiratory tract transmitted pathogen enrichment sampling mask provided by the invention can capture pathogens in the breath of a mask wearer while blocking the pathogens, so that the pathogens can be enriched to the maximum extent in a patch, the respiratory tract transmitted pathogen enrichment sampling mask is beneficial to sample collection in a long-distance travel process, a complex throat swab process is avoided, doctors and patients are protected, the detection pressure is relieved, and the effectiveness of subsequent detection and the patient investigation efficiency are improved.
Drawings
FIG. 1 is a functional schematic diagram of a respiratory tract pathogen enrichment sampling mask patch of the present invention;
FIG. 2 is a schematic structural view of the respiratory tract pathogen enrichment sampling mask of the present invention;
FIG. 3 is a chart of a colorimetric chart for color development in example 1 of the present invention;
FIG. 4 is an SDS-PAGE electrophoresis of the monoclonal antibody in example 2 of the present invention;
FIG. 5 is a graph showing the results of the ELISA titer detection of the monoclonal antibodies in example 2 of the present invention.
Description of reference numerals:
1, a mask; 2-respiratory tract transmitted pathogen enrichment sampling mask paster.
Detailed Description
The present invention is further described in detail below with reference to examples to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The present example provides a respiratory-transmitted pathogen-enriched spun film comprising a protein spun film coupled to a capture antibody that is a specific antibody capable of targeting binding to a variety of respiratory-transmitted pathogens.
Wherein the respiratory pathogens include, but are not limited to, influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, and tubercle bacillus.
Wherein the protein spinning membrane includes, but is not limited to, natural plant protein membrane, plant protein spinning membrane, animal protein spinning membrane, and insect spinning membrane.
The preparation method of the respiratory tract transmitted pathogen enrichment spinning membrane comprises the following steps: preparing a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody, and bridging the protein spinning membrane mouse-derived monoclonal antibody and the pathogen specificity mouse-derived monoclonal antibody through an anti-mouse secondary antibody to obtain a spinning membrane with the function of capturing respiratory tract transmitted pathogens: i.e., respiratory tract transmitted pathogen-enriched spun membranes.
The embodiment also provides a respiratory tract transmitted pathogen enrichment sampling mask patch and a sampling mask prepared according to the respiratory tract transmitted pathogen enrichment spinning film. In the scheme of the invention, by simultaneously capturing and enriching pathogens in the breath of a mask wearer, a complex throat swab process can be avoided, doctors and patients can be protected, and the effectiveness of subsequent detection and the investigation efficiency of the patients can be improved.
The present invention is further described in detail with reference to the following examples.
Example 1
The preparation of the protein spinning membrane comprises the following steps:
1. protein spinning
Freezing plant protein filaments, animal protein filaments and insect spinning filaments, grinding into ultra-short filaments or powder, adding a small amount of polyethylene terephthalate (PET), fully and uniformly mixing, and then spinning, filament falling and drafting to form a protein spinning net with the aperture of 50 mu m +/-10 mu m.
2. Machining
Cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot pressing plate, and carrying out vertical hot pressing to form the protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
3. Protein spinning membrane assay
The pore size, thickness and porosity of the protein spinning membrane were assayed and subjected to indirect ELISA using specific monoclonal antibodies. The ELISA assay procedure was as follows: adding a protein spinning membrane into a 24-pore plate, then adding an anti-protein spinning membrane antibody with the final concentration of 15 mu g/ml, incubating for 2h at room temperature, washing for 3 times with PBST washing liquor, then adding an enzyme-labeled anti-mouse secondary antibody, reacting for 30min at 37 ℃, washing for 5 times with PBST washing liquor, adding a precipitable substrate TMB for color development for 15min, detecting the color development condition of a protein spinning membrane, and determining that the color development grade is not less than 5, thus the protein spinning membrane is qualified. The colorimetric chart is shown in FIG. 3.
Example 2
Preparation of murine monoclonal antibody and anti-murine secondary antibody:
the preparation of the mouse-derived monoclonal antibody comprises the following steps:
1. immunization of mice
Respectively preparing protein spinning membrane antigen and pathogen antigen, emulsifying, and performing multiple-point immunization on BLAB/C mice via abdominal cavity, back subcutaneous route, etc., for 4 times with interval period of 10 days. The mice tail vein was boosted 2 days prior to fusion.
2. Cell fusion
Taking the immunized mouse spleen, preparing a spleen cell suspension, and mixing the mouse spleen cell with the cultured NS1 myeloma cell. The mouse spleen cells and NS1 myeloma cells were fused using 50% PEG 4000 as a fusion agent. The fusion cells were cultured for several days in selection medium containing 20% newborn calf serum.
3. Cloning and screening
The protein spinning membrane antigen and the pathogen antigen are respectively operated according to the following steps: diluting to 10 μ g/ml with 0.05M carbonate buffer solution with pH9.6, and coating with 100 μ L/well of ELISA plate; overnight at 4 ℃; washing the next day with Phosphate Buffer Solution (PBST) containing 0.03-0.06% Tween-20 for 5 times, and draining; adding the cell culture supernatant to be detected, incubating at 37 ℃, and detecting by adopting an indirect ELISA method. The Cutoff value was 2.1 times that of the negative control, and a positive result was obtained when the Cutoff value was higher than that of the negative control. Selecting fused cells in the positive holes of the detection result, and performing multiple times of monoclonal culture by a limiting dilution method; and (4) measuring after the single cell grows into the subclone, and freezing and storing by liquid nitrogen after the positive clone is expanded.
4. Preparation and purification of monoclonal antibody
Culturing the hybridoma cell strain, injecting into the abdominal cavity of sensitized mice, each 10 times6(ii) individual cells; observing the abdominal cavity of the mouse, and collecting ascites of the mouse within about 5-10 days; slowly adding saturated ammonium sulfate solution with equal proportion into ascites of the mouse while stirring, stirring for 1h, and standing at 4 ℃ overnight; centrifuging the mouse ascites, collecting precipitate, and dissolving with small amount of physiological saline; then removing ammonium sulfate in the solution by using a G-25 gel chromatographic column; purifying by using DEAE-52 anion exchange chromatography columnPerforming anti-infection, and eluting by using NaCl solutions with different concentrations to obtain a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody; the purity and titer of the purified antibody are determined by SDS-PAGE electrophoresis and indirect ELISA method, and the detection results are shown in figure 4 and figure 5, which shows that the purity and titer of the antibody obtain good results.
Secondly, preparing an anti-mouse secondary antibody, which comprises the following steps: the gamma globulin immune goat, horse, donkey, rabbit or chicken of the mouse is subjected to a certain immune procedure, animal serum is collected, and a specific anti-mouse antibody in the serum is extracted.
Example 3
The preparation of the respiratory tract transmitted pathogen enrichment spinning film, the mask paster and the sampling mask comprises the following steps:
1. protein spinning membrane linked pathogen specific monoclonal antibody
And respectively soaking the protein spinning membranes in anti-protein spinning membrane specific antibody solutions with final concentration of 10-30 mu g/ml, and standing overnight at 4 ℃. Washing with Phosphate Buffer Solution (PBST) containing 0.03-0.06% Tween-20 for 5 times, detecting by using an indirect ELISA method, soaking the protein spinning membrane in an anti-mouse secondary antibody solution with the final concentration of 10-30 mug/ml after the detection is qualified, and standing overnight at 4 ℃. And washing the PBST washing liquid for the next day for 5 times, soaking the PBST washing liquid in an anti-pathogen specific monoclonal antibody solution with the final concentration of 10-30 mu g/ml, incubating and washing according to the method, and detecting by using an ELISA method.
2. Protein spinning membrane slice
And draining the protein spinning membrane connected with the pathogen specific monoclonal antibody, and mechanically cutting and processing the protein spinning membrane to prepare a circular or square respiratory tract transmitted pathogen enrichment spinning membrane with the diameter of 10mm +/-2 mm.
3. Preparation of mask paster
And (3) attaching an adhesive layer such as an environment-friendly adhesive or a non-dry adhesive to one side of the prepared protein spinning membrane body to prepare the respiratory tract transmitted pathogen enrichment sampling mask patch, and referring to fig. 1.
4. Preparation of sampling mask
Referring to fig. 2, mask patch 2 was attached to the inner side of mask 1 to obtain a respiratory pathogen-enriched sampling mask.
While embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields of application of the invention, and further modifications may readily be effected by those skilled in the art, and the invention is thus not limited to the specific details without departing from the generic concept defined in the claims and the equivalent scope.
Claims (10)
1. A respiratory-transmitted pathogen-enriched spun film comprising a protein spun film coupled to a capture antibody, the capture antibody being a specific antibody capable of targeting binding to a plurality of respiratory-transmitted pathogens.
2. The respiratory infectious pathogen-enriched spinning membrane according to claim 1, wherein the respiratory infectious pathogen comprises at least influenza virus, coronavirus, measles virus, respiratory syncytial virus, meningococcus, tubercle bacillus.
3. The respiratory tract-transmitted pathogen-enriched spun membrane of claim 2, wherein the protein spun membrane comprises one or more of a natural plant protein membrane, a plant protein spun membrane, an animal protein spun membrane, and an insect spinning membrane.
4. The respiratory tract transmitted pathogen-enriched spun film according to claim 3, which is prepared by the steps of: preparing a protein spinning membrane mouse-derived monoclonal antibody and a pathogen specificity mouse-derived monoclonal antibody, and bridging the protein spinning membrane mouse-derived monoclonal antibody and the pathogen specificity mouse-derived monoclonal antibody through an anti-mouse secondary antibody to obtain the respiratory tract transmitted pathogen enriched spinning membrane.
5. The respiratory tract transmitted pathogen-enriched spun film according to claim 4, wherein the protein spun film is prepared by a method comprising the steps of: cutting the protein spinning net into squares with the same size, and vertically overlapping and laying the squares into a non-woven fabric base material; then evenly spraying water mist, placing the non-woven fabric substrate in the middle of a hot pressing plate, and carrying out vertical hot pressing to form the protein spinning film with the thickness of 0.5mm +/-0.05 mm and the porosity of 2-5%.
6. The respiratory tract transmitted pathogen-enriched spun film according to claim 5, wherein the preparation method of the protein spun film murine monoclonal antibody comprises the following steps: immunizing a mouse with the protein spinning membrane antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting a specific anti-protein spinning membrane antigen to obtain a stable hybridoma cell strain; then, the cell is inoculated to the abdominal cavity of a mouse, ascites is prepared, and the specific protein spinning membrane mouse-derived monoclonal antibody is extracted from the ascites.
7. The respiratory tract-transmitted pathogen-enriched spinning membrane according to claim 6, wherein the method for preparing the pathogen-specific murine monoclonal antibody comprises the steps of: immunizing a mouse with a pathogen specific antigen, taking a spleen cell of the mouse, fusing with a myeloma cell of the mouse, and screening a monoclonal antibody hybridoma secreting the specific anti-pathogen antigen to obtain a stable hybridoma cell strain; then, the cells are inoculated into the abdominal cavity of a mouse, ascites is prepared, and a specific pathogen-specific murine monoclonal antibody is extracted from the ascites.
8. The respiratory tract transmissible pathogen enriching spinning membrane according to claim 7, wherein the preparation method of the anti-mouse secondary antibody comprises the following steps: the gamma globulin immune goat, horse, donkey, rabbit or chicken of the mouse is subjected to a certain immune procedure, animal serum is collected, and a specific anti-mouse antibody in the serum is extracted.
9. The respiratory tract transmitted pathogen enrichment sampling mask paster is characterized in that the respiratory tract transmitted pathogen enrichment sampling mask paster is prepared by attaching an adhesive layer to one side of the respiratory tract transmitted pathogen enrichment spinning film in any one of the steps 1-8.
10. A respiratory tract transmitted pathogen enrichment sampling mask, which is characterized in that the inner side of the mask is provided with the sampling mask paster according to claim 9.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010548759.9A CN111938230B (en) | 2020-06-16 | 2020-06-16 | Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask |
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| CN202010548759.9A CN111938230B (en) | 2020-06-16 | 2020-06-16 | Respiratory tract transmitted pathogen enrichment spinning membrane, sampling mask paster and sampling mask |
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| CN111938230B CN111938230B (en) | 2022-10-14 |
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| CN113519939A (en) * | 2021-09-17 | 2021-10-22 | 中南大学 | Mouth-nose mask for visible target collection of novel coronavirus sample and visible warning |
| TWI766603B (en) * | 2020-10-06 | 2022-06-01 | 康科特股份有限公司 | Facial mask for collecting virus specimen from respirator tracts of human and animals |
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| CN104560874A (en) * | 2014-12-04 | 2015-04-29 | 南昌大学 | Method for capturing memory B cells aimed at respiratory syncytial virus by virtue of Elisa method |
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| CN111938230B (en) | 2022-10-14 |
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