CN111925965A - Biological sewage treatment composite microbial inoculum and preparation method thereof - Google Patents
Biological sewage treatment composite microbial inoculum and preparation method thereof Download PDFInfo
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 64
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
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- 239000004626 polylactic acid Substances 0.000 claims abstract description 32
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- LKDRXBCSQODPBY-AMVSKUEXSA-N L-(-)-Sorbose Chemical compound OCC1(O)OC[C@H](O)[C@@H](O)[C@@H]1O LKDRXBCSQODPBY-AMVSKUEXSA-N 0.000 claims description 5
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- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 2
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
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- 229910052698 phosphorus Inorganic materials 0.000 description 6
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 239000012190 activator Substances 0.000 description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 4
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- 239000012752 auxiliary agent Substances 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C12N11/096—Polyesters; Polyamides
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- Genetics & Genomics (AREA)
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- General Health & Medical Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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Abstract
The invention provides a biological sewage treatment composite microbial inoculum, which comprises a carrier, mixed strains and an activating agent; the carrier is polylactic acid fiber particles. The invention also provides a preparation method of the biological sewage treatment composite microbial inoculum, which comprises the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding. The invention has the beneficial effects that: the dosage of the complex microbial inoculum required for treating the sewage of unit volume is small, and the average total adding amount is only 17g/m3The sewage treatment cost is low, which is beneficial to accelerating the popularization of the biological water treatment technology; after the sewage is treated, the COD removal rate reaches 99.5 percent; the NH3-N removal rate reaches 98.1 percent, and the TP removal rate reaches 99.3 percent; the water quality can meet the production water recycling standard of enterprises, the subsequent water treatment is not needed, and the production cost of the enterprises is reduced.
Description
Technical Field
The invention relates to the field of garbage treatment, in particular to a biological sewage treatment composite microbial inoculum and a preparation method thereof.
Background
With the increasing expansion of urban population and the continuous development of industry and agriculture, water environment pollution accidents occur frequently, and the health and even life of people and livestock are seriously harmed. Many lakes and reservoirs cause eutrophication of water bodies due to the discharge of nitrogen and phosphorus, and seriously threaten the production life and ecological balance of human beings. Ammonia nitrogen is one of the main factors causing water eutrophication. In order to meet the continuously improved requirements of the public on the environmental quality, the nation sets up more and more strict emission standards for nitrogen, and research and development of economic and efficient nitrogen removal treatment technology becomes a key point and a hotspot of research in the field of water pollution control engineering. Although there are many methods for removing ammonia effectively, such as physical methods like reverse osmosis, distillation, soil irrigation; the chemical method comprises an ion exchange method, ammonia stripping, a chemical precipitation method, breakpoint chlorination, electrodialysis, electrochemical treatment and catalytic cracking; the biological method has the advantages of nitrification and algae cultivation, but the physical method has poor treatment effect, and compared with the chemical method, the biological method has the following advantages: 1) each chemical product is a highly targeted product, and can be out of work when meeting other chemical substances, and the removal of pollutants by biological agents has spectrum; 2) the chemical product can temporarily eliminate certain harmful substances and cover odor, but cannot prevent the generation of the harmful substances; 3) after the chemical product is used, residues are left in the water body, which may cause secondary pollution. The biological preparation contains natural microorganisms without pathogenic bacteria and pathogens, the microorganisms take organic nutrients in the sewage as food under the catalysis of enzyme, and after the sewage is purified, the microorganisms are gradually reduced along with the reduction of the pollutants until the microorganisms are eliminated; 4) the paint is non-toxic, non-corrosive, convenient to use, basically does not need to add equipment or engineering, and saves the capital investment.
The sewage mainly comprises domestic sewage and industrial wastewater. The components of industrial sewage are relatively complex, particularly a large amount of artificially synthesized compounds enter the environment, the substances mainly comprise ammonia nitrogen, sulfide and phosphorus-containing compounds, and the substances cannot be decomposed and utilized by microorganisms in a short time due to the complexity of the structures of the substances, so that the microorganisms cultured and domesticated by the activated sludge in the traditional wastewater treatment method cannot effectively remove the pollutants, the substances are accumulated in the environment for a long time, great pollution is caused to the ecological environment which depends on the survival of people, and great harm is brought to the physical and mental health of human beings. A certain part of industrial pollution enterprises in China are penalized and are not willing to invest and treat wastewater, and even if a sewage treatment device runs abnormally.
The microbial compound bacterial agent is prepared by combining or mixing and culturing several microbes with different degradation functions and mutual or symbiotic relations according to a proper proportion. The method improves the treatment efficiency of the organic pollutants difficult to degrade by adding the functional microbial agent into the wastewater treatment system, and is a common biological strengthening technology at present. The applicant finds that the dosage of the complex microbial inoculum required by the existing complex microbial inoculum for treating the sewage with unit volume is higher, so that the cost of biological sewage treatment is higher, and the popularization of the water treatment technology is limited; on the other hand, after the sewage is treated by the existing biological sewage treatment composite microbial inoculum, the COD removal rate, ammonia nitrogen removal rate and total phosphorus removal rate are not good, the production water reuse standard of enterprises cannot be met, and the subsequent water treatment process and cost are increased.
Chinese patent CN110331105A discloses a sewage treatment composite bacterial agent and a preparation method thereof, which adopts the compounding of various bacterial powders to degrade organic wastewater and reduce COD, NH4-N and total phosphorus indexes of sewage. The patent has the following defects: in order to achieve the best treatment effect, the application amount of the sewage treatment composite microbial inoculum is too high, and the sewage treatment composite microbial inoculum treats 1m3The dosage of the complex microbial inoculum required to be applied to sewage with the volume is 8kg, and the sewage treatment cost is high; moreover, after the sewage is treated, the COD removal rate is 93.36 percent, and NH is added4The N removal rate is 90.28 percent, the total phosphorus removal rate is 78.96 percent, and the indexes are low in total and cannot meet the requirementsThe production water reuse standard of enterprises increases the flow and the cost of subsequent water treatment.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides a biological sewage treatment composite microbial inoculum and a preparation method thereof, so as to realize the following purposes:
(1) the complex microbial inoculum for treating the biological sewage and the preparation method thereof are provided, the complex microbial inoculum required for treating the sewage with unit volume is small in dosage, the sewage treatment cost is low, and the popularization of the biological water treatment technology is facilitated;
(2) the biological sewage treatment composite microbial inoculum and the preparation method thereof have high COD removal rate, ammonia nitrogen removal rate and total phosphorus removal rate, can meet the production water reuse standard of enterprises, do not need subsequent water treatment, and reduce the production cost of the enterprises.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a composite bacterial preparation for treating biological sewage is composed of carrier, mixed bacterial strain and activating assistant.
The carrier is polylactic acid fiber particles.
The mixed strain comprises the following raw material strains in parts by mass: 10-15 parts of pichia pastoris, 20-25 parts of bacillus subtilis, 5-10 parts of actinomycetes, 15-20 parts of thiobacillus denitrificans, 10-20 parts of lactobacillus acidophilus, 5-10 parts of pseudomonas stutzeri, 20-25 parts of bacillus mucilaginosus and 5-10 parts of bacillus laterosporus.
The activating aid comprises the following raw materials in parts by mass: 20-30 parts of glucose, 10-12 parts of glycine chelated zinc, 3-7 parts of L-sorbose, 10-15 parts of malt wort agar and 5-10 parts of yeast glucan.
According to the polylactic acid fiber particles, 10-15 meshes of polylactic acid particles are placed in 5% hydrochloric acid solution with 2 times of volume, and ultrasonic dispersion is carried out for 20-30 min under the conditions that the pressure is 1.0-1.5 MPa and the temperature is 30-35 ℃; then heating to 60-75 ℃, and keeping the temperature for 1-1.5 h; and then washing the polylactic acid fiber particles to be neutral by using enough deionized water, placing the polylactic acid fiber particles in a hot air environment at 50 ℃, and drying the polylactic acid fiber particles at a low temperature until the moisture content is less than 5%.
The wort agar is put into deionized water with the same volume of 50-60 ℃, and is magnetically stirred for 10-15 min; adding chitosan powder, soybean meal and rhein, heating to 120-130 ℃, and performing ultrasonic dispersion for 20-30 min; then modifying and shearing the mixture at a high speed of 600-750 RPM for 3-5 min; and cooling to 5-10 ℃, filtering out the solid material of the mixture, and drying until the moisture content is less than 5%.
The wort agar: chitosan powder: soybean meal: the weight ratio of the rhein is 20:3:5: 1.
A preparation method of a biological sewage treatment composite microbial inoculum comprises the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding.
Culturing the strains, respectively placing the strains into sterile active culture medium, and adding glucose and KH2PO4And (3) culturing the cells in gelatin peptone at 30 ℃ and 250RPM for 30 h.
The glucose: KH (Perkin Elmer)2PO4: the weight ratio of the gelatin peptone is 10:3: 5.
The strain loading step of mixing the strains cultured by the strains to prepare a mixed strain; and mixing the mixed strain with a carrier, stirring at 20-30 RPM for 30-50 min, and standing for 2-3 hours to obtain the load microbial inoculum.
The strain carrier comprises: the weight ratio of the mixed strains is 1: 3.
And (3) activating the strain, mixing the supported microbial inoculum and an activating agent, heating to 20-30 ℃, and ultrasonically oscillating for 30 min.
The loading microbial inoculum: the activator has a weight ratio of 5: 1.
The activating agent is a mixture of fructose, alanine and hydroxycitric acid, wherein the ratio of fructose: alanine: the weight ratio of the hydroxycitric acid is 3:1: 1.
And drying, namely drying the materials until the water content is less than 3%.
The microbial inoculum is compounded, and the raw materials are uniformly mixed according to the predetermined parts, so that the biological sewage treatment composite microbial inoculum and the preparation method thereof are prepared.
The strain carrier comprises: mixing strains: the weight ratio of the activating agent is 3:9: 1.
Compared with the prior art, the invention has the beneficial effects that:
(1) the compound microbial inoculum for treating the biological sewage has small dosage of the compound microbial inoculum required by treating the sewage of unit volume, the average adding total amount is only 17g/m3, the sewage treatment cost is low, and the popularization of the biological water treatment technology is facilitated;
(2) after the biological sewage treatment composite microbial inoculum disclosed by the invention is used for treating sewage, the COD removal rate reaches 99.5%; NH (NH)3The N removal rate reaches 98.1 percent, and the TP removal rate reaches 99.3 percent;
(3) after the biological sewage treatment composite microbial inoculum disclosed by the invention is used for treating sewage, the detection of various indexes of water quality is shown in the following table:
(4) the biological sewage treatment composite microbial inoculum has high strain activity, can be repeatedly domesticated and cultured, and realizes the recycling of the strain;
(5) after the biological sewage treatment composite microbial inoculum disclosed by the invention is used for treating sewage, the water quality of the sewage can meet the production water reuse standard of enterprises, subsequent water treatment is not needed, and the production cost of the enterprises is reduced;
(6) the biological sewage treatment composite microbial inoculum has stable system environment and good water treatment effect in the sewage treatment process.
(7) Compared with the prior art, the biological sewage treatment composite microbial inoculum has the advantages of large treatment capacity and high treatment capacity.
Detailed Description
In order to more clearly understand the technical features, objects, and effects of the present invention, specific embodiments of the present invention will now be described.
Example 1
A composite bacterial preparation for treating biological sewage is composed of carrier, mixed bacterial strain and activating assistant.
The carrier is polylactic acid fiber particles.
The mixed strain comprises the following raw material strains in parts by mass: 10 parts of pichia pastoris, 20 parts of bacillus subtilis, 10 parts of actinomycetes, 15 parts of thiobacillus denitrificans, 10 parts of lactobacillus acidophilus, 5 parts of pseudomonas stutzeri, 25 parts of bacillus mucilaginosus and 5 parts of bacillus laterosporus.
The activating aid comprises the following raw materials in parts by mass: 20 parts of glucose, 10 parts of glycine chelated zinc, 3 parts of L-sorbose, 15 parts of malt extract agar and 5 parts of yeast glucan.
The polylactic acid fiber particles are prepared by placing 10-mesh polylactic acid particles into 5% hydrochloric acid solution with 2 times of volume, and ultrasonically dispersing for 20min under the conditions that the pressure is 1.0MPa and the temperature is 30 ℃; then heating to 60 ℃, and keeping the temperature for 1 h; and then washing the polylactic acid fiber particles to be neutral by using enough deionized water, placing the polylactic acid fiber particles in a hot air environment at 50 ℃, and drying the polylactic acid fiber particles at a low temperature until the moisture content is less than 5%.
The wort agar is put into deionized water with the same volume at 50 ℃, and is magnetically stirred for 10 min; adding chitosan powder, soybean powder, and rhein, heating to 120 deg.C, and ultrasonically dispersing for 20 min; the mixture was then high speed modified sheared at 600RPM for 3 min; cooling to 5 ℃, filtering out the solid material of the mixture, and drying until the moisture content is less than 5%.
The wort agar: chitosan powder: soybean meal: the weight ratio of the rhein is 20:3:5: 1.
A preparation method of a biological sewage treatment composite microbial inoculum comprises the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding.
Culturing the strains, respectively placing the strains into sterile active culture medium, and adding glucose and KH2PO4And (3) culturing the cells in gelatin peptone at 30 ℃ and 250RPM for 30 h.
The glucose: KH (Perkin Elmer)2PO4: the weight ratio of the gelatin peptone is 10:3: 5.
The strain loading step of mixing the strains cultured by the strains to prepare a mixed strain; and mixing the mixed strain with a carrier, stirring at 20RPM for 30min, and standing for 2 hours to obtain the load microbial inoculum.
The strain carrier comprises: the weight ratio of the mixed strains is 1: 3.
And (3) activating the strain, mixing the supported microbial inoculum and an activating agent, heating to 20 ℃, and ultrasonically oscillating for 30 min.
The loading microbial inoculum: the activator has a weight ratio of 5: 1.
The activating agent is a mixture of fructose, alanine and hydroxycitric acid, wherein the ratio of fructose: alanine: the weight ratio of the hydroxycitric acid is 3:1: 1.
And drying, namely drying the materials until the water content is less than 3%.
The microbial inoculum is compounded, and the raw materials are uniformly mixed according to the predetermined parts, so that the biological sewage treatment composite microbial inoculum and the preparation method thereof are prepared.
The strain carrier comprises: mixing strains: the weight ratio of the activating agent is 3:9: 1.
Example 2
A composite bacterial preparation for treating biological sewage is composed of carrier, mixed bacterial strain and activating assistant.
The carrier is polylactic acid fiber particles.
The mixed strain comprises the following raw material strains in parts by mass: 15 parts of pichia pastoris, 25 parts of bacillus subtilis, 5 parts of actinomycetes, 20 parts of thiobacillus denitrificans, 20 parts of lactobacillus acidophilus, 10 parts of pseudomonas stutzeri, 20 parts of bacillus mucilaginosus and 10 parts of bacillus laterosporus.
The activating aid comprises the following raw materials in parts by mass: 30 parts of glucose, 12 parts of glycine chelated zinc, 7 parts of L-sorbose, 10 parts of malt extract agar and 10 parts of yeast glucan.
The polylactic acid fiber particles are prepared by placing 15-mesh polylactic acid particles into 5% hydrochloric acid solution with 2 times of volume, and ultrasonically dispersing for 30min under the conditions that the pressure is 1.5MPa and the temperature is 35 ℃; then heating to 75 ℃, and keeping the temperature for 1.5 h; and then washing the polylactic acid fiber particles to be neutral by using enough deionized water, placing the polylactic acid fiber particles in a hot air environment at 50 ℃, and drying the polylactic acid fiber particles at a low temperature until the moisture content is less than 5%.
The wort agar is put into deionized water with the same volume at 60 ℃, and is magnetically stirred for 15 min; adding chitosan powder, soybean powder, and rhein, heating to 130 deg.C, and ultrasonically dispersing for 30 min; the mixture was then high speed modified sheared at 750RPM for 5 min; cooling to 10 deg.C, filtering to obtain solid material, and oven drying until the water content is less than 5%.
The wort agar: chitosan powder: soybean meal: the weight ratio of the rhein is 20:3:5: 1.
A preparation method of a biological sewage treatment composite microbial inoculum comprises the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding.
Culturing the strains, respectively placing the strains into sterile active culture medium, and adding glucose and KH2PO4And (3) culturing the cells in gelatin peptone at 30 ℃ and 250RPM for 30 h.
The glucose: KH (Perkin Elmer)2PO4: the weight ratio of the gelatin peptone is 10:3: 5.
The strain loading step of mixing the strains cultured by the strains to prepare a mixed strain; and mixing the mixed strain with a carrier, stirring at 30RPM for 50min, and standing for 3 hours to obtain the load microbial inoculum.
The strain carrier comprises: the weight ratio of the mixed strains is 1: 3.
And (3) activating the strain, mixing the supported microbial inoculum and an activating agent, heating to 30 ℃, and ultrasonically oscillating for 30 min.
The loading microbial inoculum: the activator has a weight ratio of 5: 1.
The activating agent is a mixture of fructose, alanine and hydroxycitric acid, wherein the ratio of fructose: alanine: the weight ratio of the hydroxycitric acid is 3:1: 1.
And drying, namely drying the materials until the water content is less than 3%.
The microbial inoculum is compounded, and the raw materials are uniformly mixed according to the predetermined parts, so that the biological sewage treatment composite microbial inoculum and the preparation method thereof are prepared.
The strain carrier comprises: mixing strains: the weight ratio of the activating agent is 3:9: 1.
Example 3
A composite bacterial preparation for treating biological sewage is composed of carrier, mixed bacterial strain and activating assistant.
The carrier is polylactic acid fiber particles.
The mixed strain comprises the following raw material strains in parts by mass: 13 parts of pichia pastoris, 21 parts of bacillus subtilis, 7 parts of actinomycetes, 16 parts of thiobacillus denitrificans, 12 parts of lactobacillus acidophilus, 8 parts of pseudomonas stutzeri, 22 parts of bacillus mucilaginosus and 7 parts of bacillus laterosporus.
The activating aid comprises the following raw materials in parts by mass: 25 parts of glucose, 12 parts of glycine chelated zinc, 5 parts of L-sorbose, 12 parts of malt extract agar and 6 parts of yeast glucan.
The polylactic acid fiber particles are prepared by placing 15-mesh polylactic acid particles into 5% hydrochloric acid solution with 2 times of volume, and ultrasonically dispersing for 30min under the conditions that the pressure is 1.2MPa and the temperature is 30 ℃; then heating to 70 ℃, and keeping the temperature for 1.5 h; and then washing the polylactic acid fiber particles to be neutral by using enough deionized water, placing the polylactic acid fiber particles in a hot air environment at 50 ℃, and drying the polylactic acid fiber particles at a low temperature until the moisture content is less than 5%.
The wort agar is put into deionized water with the same volume at 55 ℃, and is magnetically stirred for 12 min; adding chitosan powder, soybean powder, and rhein, heating to 125 deg.C, and ultrasonically dispersing for 25 min; the mixture was then high speed modified sheared at 700RPM for 4 min; cooling to 5 ℃, filtering out the solid material of the mixture, and drying until the moisture content is less than 5%.
The wort agar: chitosan powder: soybean meal: the weight ratio of the rhein is 20:3:5: 1.
A preparation method of a biological sewage treatment composite microbial inoculum comprises the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding.
Culturing the strains, respectively placing the strains into sterile active culture medium, and adding glucose and KH2PO4A gelatin peptone and a solvent.The culture was carried out at 250RPM for 30h at 30 ℃.
The glucose: KH (Perkin Elmer)2PO4: the weight ratio of the gelatin peptone is 10:3: 5.
The strain loading step of mixing the strains cultured by the strains to prepare a mixed strain; and mixing the mixed strain with a carrier, stirring at 25RPM for 40min, and standing for 2.5 hours to obtain the load microbial inoculum.
The strain carrier comprises: the weight ratio of the mixed strains is 1: 3.
And (3) activating the strain, mixing the supported microbial inoculum and an activating agent, heating to 25 ℃, and ultrasonically oscillating for 30 min.
The loading microbial inoculum: the activator has a weight ratio of 5: 1.
The activating agent is a mixture of fructose, alanine and hydroxycitric acid, wherein the ratio of fructose: alanine: the weight ratio of the hydroxycitric acid is 3:1: 1.
And drying, namely drying the materials until the water content is less than 3%.
The microbial inoculum is compounded, and the raw materials are uniformly mixed according to the predetermined parts, so that the biological sewage treatment composite microbial inoculum and the preparation method thereof are prepared.
The strain carrier comprises: mixing strains: the weight ratio of the activating agent is 3:9: 1.
Example 4
A leachate treatment test is carried out by using the biological sewage treatment composite microbial inoculum described in the embodiment 1 to 3, meanwhile, a comparison test 1 to 3 is set, and water quality indexes after sewage treatment by using the biological sewage treatment composite microbial inoculum described in the embodiment 1 to 3 and the comparison example 1 to 3 are compared.
The method comprises the following steps of selecting production sewage of a certain enterprise as a test sample, wherein the water quality index of the test sample is shown in the following table:
comparative example 1: the biological sewage treatment composite bacterial agent and the preparation method thereof in the embodiment 3 are adopted, and the difference is that: the biological sewage treatment composite microbial inoculum does not adopt a polylactic acid fiber particle loading method, and is prepared by only mixing various strains and auxiliary agents together.
Comparative example 2: the biological sewage treatment composite bacterial agent and the preparation method thereof in the embodiment 3 are adopted, and the difference is that: the 'activating aid' in the raw material components of the biological sewage treatment composite microbial inoculum is omitted.
Comparative example 3: the biological sewage treatment composite bacterial agent and the preparation method thereof in the embodiment 3 are adopted, and the difference is that: the 'strain activation step' in the preparation method of the biological sewage treatment composite microbial agent is omitted.
The compound microbial inoculum for treating the biological sewage, which is described in the examples 1 to 3 and the comparative examples 1 to 3, is added into each test sample, and the adding amount is as follows: 7g/m on the first day3The next day 5g/m35g/m on day three3. The comparison conditions of various detection indexes of the treated test sample sewage quality are shown in the following table:
in addition, through detection, the biological sewage treatment composite microbial inoculum has high strain activity and can be repeatedly domesticated and cultured; in addition, the system environment is stable in the sewage treatment process, and the water treatment effect is good; after the sewage is treated, the production water recycling standard of enterprises can be met, subsequent water treatment is not needed, and the production cost of the enterprises is effectively reduced.
All percentages used in the present invention are mass percentages unless otherwise indicated.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A biological sewage treatment composite bacterial agent is characterized by comprising a carrier, mixed strains and an activating assistant; the carrier is polylactic acid fiber particles.
2. The composite microbial inoculum for biological sewage treatment according to claim 1, wherein the mixed strains comprise the following raw material strains in parts by mass: 10-15 parts of pichia pastoris, 20-25 parts of bacillus subtilis, 5-10 parts of actinomycetes, 15-20 parts of thiobacillus denitrificans, 10-20 parts of lactobacillus acidophilus, 5-10 parts of pseudomonas stutzeri, 20-25 parts of bacillus mucilaginosus and 5-10 parts of bacillus laterosporus.
3. The biological sewage treatment composite bacterial agent according to claim 1, wherein the activating agent comprises the following raw materials in parts by mass: 20-30 parts of glucose, 10-12 parts of glycine chelated zinc, 3-7 parts of L-sorbose, 10-15 parts of malt wort agar and 5-10 parts of yeast glucan.
4. The biological sewage treatment composite bacterial agent according to claim 1, wherein the polylactic acid fiber particles are prepared by placing polylactic acid particles into a 5% hydrochloric acid solution, keeping the pressure at 1.0-1.5 MPa, and performing ultrasonic dispersion for 20-30 min at 30-35 ℃; then heating to 60-75 ℃, and keeping the temperature for 1-1.5 h; and then washing the polylactic acid fiber particles to be neutral by using enough deionized water, placing the polylactic acid fiber particles in a hot air environment at 50 ℃, and drying the polylactic acid fiber particles at a low temperature until the moisture content is less than 5%.
5. The biological sewage treatment composite bacterial agent according to claim 3, wherein the wort agar is put into deionized water with the same volume, and is stirred uniformly by magnetic force; adding chitosan powder, soybean meal and rhein, heating to 120-130 ℃, and performing ultrasonic dispersion for 20-30 min; then modifying and shearing the mixture at a high speed of 600-750 RPM for 3-5 min; and cooling to 5-10 ℃, filtering out the solid material of the mixture, and drying until the moisture content is less than 5%.
6. The biological sewage treatment composite bacterial agent according to claim 5, wherein the ratio of wort agar: chitosan powder: soybean meal: the weight ratio of the rhein is 20:3:5: 1.
7. A preparation method of a biological sewage treatment composite bacterial agent is characterized by comprising the following steps: strain culture, strain loading, strain activation, drying and microbial inoculum compounding.
8. The method for preparing a composite bacterial agent for biological sewage treatment according to claim 7, wherein the bacterial species are cultured by placing raw bacterial species into sterile active culture medium respectively, and adding glucose and KH2PO4Culturing with gelatin peptone at 30 deg.C and 250RPM for 30 h;
the glucose: KH (Perkin Elmer)2PO4: the weight ratio of the gelatin peptone is 10:3: 5.
9. The method for preparing a composite bacterial agent for biological sewage treatment according to claim 7, wherein the bacterial strain is loaded, and the bacterial strains cultured by the bacterial strains are mixed to prepare a mixed bacterial strain; mixing the mixed strain with a carrier, stirring at 20-30 RPM for 30-50 min, and standing for 2-3 hours to obtain a loaded microbial inoculum;
the strain carrier comprises: the weight ratio of the mixed strains is 1: 3.
10. The preparation method of the composite microbial inoculum for biological sewage treatment according to claim 7, wherein the strain is activated, the supported microbial inoculum is mixed with an activating agent, the mixture is heated to 20-30 ℃, and the ultrasonic oscillation is carried out for 30 min;
the activating agent is a mixture of fructose, alanine and hydroxycitric acid, wherein the ratio of fructose: alanine: the weight ratio of the hydroxycitric acid is 3:1: 1.
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