CN111925942A - Application of microalgae carbon sequestration enhancer and culture medium containing enhancer - Google Patents

Application of microalgae carbon sequestration enhancer and culture medium containing enhancer Download PDF

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CN111925942A
CN111925942A CN202010815304.9A CN202010815304A CN111925942A CN 111925942 A CN111925942 A CN 111925942A CN 202010815304 A CN202010815304 A CN 202010815304A CN 111925942 A CN111925942 A CN 111925942A
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microalgae
culture medium
carbon sequestration
enhancer
medium
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孙中亮
王强
周创创
陈辉
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Henan University
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Abstract

The invention relates to an application of a microalgae carbon sequestration enhancer in improving the microalgae carbon sequestration efficiency; the culture medium comprises a microalgae carbon sequestration enhancer, wherein the microalgae carbon sequestration enhancer is one or a combination of methanol, dimethyl ether of polyethylene glycol, propylene carbonate or N-methylpyrrolidone; also relates to a method for culturing microalgae by using the culture medium to improve the carbon sequestration efficiency of the microalgae. The microalgae carbon sequestration enhancer and the culture medium containing the same provided by the invention can improve the carbon sequestration efficiency of microalgae and do not poison the microalgaeIs used and can not be absorbed and consumed by the microalgae. By using the culture medium and the method of the invention, the cultured algae cells have high carbon fixation efficiency, high carbonic anhydrase activity and high photosynthetic oxygen release rate, and are beneficial to treating CO-containing by using microalgae2Thereby achieving the effect of emission reduction.

Description

Application of microalgae carbon sequestration enhancer and culture medium containing enhancer
Technical Field
The invention relates to the field of environmental protection and microalgae culture, in particular to application of a microalgae carbon sequestration enhancer in improving microalgae carbon sequestration efficiency, a culture medium capable of improving microalgae carbon sequestration efficiency and a method for improving microalgae carbon sequestration efficiency by culturing microalgae with the culture medium
Background
Emission reduction of CO by microalgae biological method2Has important significance for alleviating greenhouse effect and developing low-carbon economy. However, the microalgae carbon sequestration industry is still in the starting stage at present, and some technical bottlenecks exist, such as low-concentration CO similar to flue gas2The problems of low solubility of gas in water, slow carbon source conversion rate of microalgae and the like need to be solved.
At present, the culture of microalgae is mostly carried out by introducing CO into a culture solution in a bubbling manner2As carbon source, in view of the low concentration of CO2The solubility of (a) is low and the residence time of bubbles in the liquid phase is short, the growth of microalgae is often limited by insufficient supply of carbon source. The patents CN200510126465.2, CN201210138598.1 and CN201210138845.8 invent the in-situ supplement of CO in a microalgae culture pond from the aspects of prolonging the gas-liquid contact time and increasing the gas-liquid contact area2The trap carbon supply technology and the horizontal immersion cover carbon supply technology strengthen the mass transfer process and greatly improve the CO supply at the carbon supply site2The absorption rate of (c). But is limited by the physicochemical properties of the culture broth, CO2The concentration of total inorganic carbon formed by dissolving in the liquid phase is still not high, especially in a near-neutral pH environment, part of carbon source is escaped to the air in the culture solution flowing out from the carbon supplementing site, and part of the carbon source is converted into carbonate, so that the carbon source limitation of microalgae growth is caused.
Therefore, there is a need for a solution to increase CO in microalgae cultivation2Absorption and conversion efficiency of (a).
Disclosure of Invention
To solve the above problems, the present team has carefully studied the improvement of CO in the liquid phase2Solubility methods, screening for possible CO2Of absorbent capacity and comparison with different absorbentsHenry coefficient (according to Henry's law, the smaller the Henry coefficient, CO2The higher the solubility is), the higher the absorption capacity of methanol, propylene carbonate, N-methyl pyrrolidone, polyethylene glycol dimethyl ether and the like, the low toxicity and the low cost are found, if the methanol, propylene carbonate, N-methyl pyrrolidone, polyethylene glycol dimethyl ether and the like can be applied to microalgae culture, the usable concentration range is wider, the total inorganic carbon concentration in the culture solution can be obviously improved, the microalgae cells are not poisoned, and the efficiency of microalgae biological carbon fixation is improved. We refer to such substances as microalgae carbon sequestration enhancers.
Based on the research, the invention provides the application of the microalgae carbon sequestration enhancer in improving the microalgae carbon sequestration efficiency.
The invention also provides a culture medium capable of improving the carbon sequestration efficiency of microalgae, which comprises a microalgae carbon sequestration enhancer, wherein the microalgae carbon sequestration enhancer is one or a combination of methanol, polyethylene glycol dimethyl ether, propylene carbonate or N-methyl pyrrolidone.
In a specific embodiment, methanol is contained in the culture medium at a concentration of 0.05 to 1.0% volume fraction, or polyethylene glycol dimethyl ether, propylene carbonate or N-methylpyrrolidone, or a combination thereof at a concentration of 0.5 to 10 mmol/L. Preferably, the concentration of propylene carbonate is 4-6 mmol/L.
In a preferred embodiment, the pH of the medium is 6 to 8.
In a specific embodiment, a basal medium composition is included, the basal medium being a medium for culturing photosynthetic microalgae.
In a specific embodiment, the basal medium is BG11 medium, SE culture, or f/2 medium.
The invention changes the physicochemical property of the culture medium and improves the CO pairing effect of the culture medium by adding the microalgae carbon-fixing enhancer into the culture medium2The absorption capacity of the microalgae increases the inorganic carbon concentration in the culture environment and promotes the microalgae to improve CO2The conversion efficiency of (a).
The invention also provides a method for improving the carbon sequestration efficiency of microalgae, which comprises the step of culturing the microalgae by using the culture medium.
In a specific embodiment, when the microalgae are cultured, a culture solution is aerated to contain CO2The gas of (2).
In one embodiment, the CO-containing compound2The gas is flue gas or industrial CO2Gas, or mixed with CO2Or a combination thereof.
The method of the invention is applicable to various microalgae culture modes, for example, can be used for closed culture, including photobioreactors, such as tubular, columnar and plate photobioreactors; it can also be used for open culture, such as open raceway pond or round culture pond.
The culture medium and the method of the invention can be used for various photosynthetic algae, for example, microalgae is green algae, diatom, blue algae, chrysophyceae and the like, and preferably chlorella, scenedesmus, phaeodactylum tricornutum, globulus and other dinoflagellates.
In a specific embodiment, the method further comprises the step of harvesting the cultured microalgae. Harvesting of microalgae can be in the stationary phase or the logarithmic phase. According to the practical culture experience, the preferable period for harvesting the microalgae is 8 days of batch culture in the closed culture mode and 6 days of batch culture in the open culture mode.
The microalgae carbon sequestration enhancer and the culture medium containing the same provided by the invention can improve the carbon sequestration efficiency of microalgae, have no toxic action on the microalgae, and cannot be absorbed and consumed by the microalgae. By using the culture medium and the method of the invention, the cultured algae cells have high carbon fixation efficiency, high carbonic anhydrase activity and high photosynthetic oxygen evolution rate. Is beneficial to the treatment of CO-containing microalgae2Thereby achieving the effect of emission reduction
Drawings
FIG. 1 is a statistical chart of the total inorganic carbon source concentration in a culture solution of chlorella cultured by adding different types of enhancers to BG11 culture medium.
FIG. 2 is a statistical chart of carbon fixation efficiency when different types of enhancers are added to BG11 medium to culture Chlorella.
FIG. 3 is a statistical chart showing the change in total organic carbon concentration in a culture solution before and after Chlorella is cultured by adding different types of enhancers to BG11 medium.
FIG. 4 is a statistical chart of the photosynthetic oxygen release rate of cells cultured with chlorella by adding different types of enhancers to BG11 medium.
FIG. 5 is a statistical chart of extracellular carbonic anhydrase activity of Chlorella cultured by adding different kinds of enhancers to BG11 medium.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
Example 1
Culturing microalgae indoors with columnar glass photoreactor (height 50cm, inner diameter 6cm), ventilating with aerator stone (aperture 30-60 μm) commonly used in the art, and providing light intensity of 100 μmol/m with 8 fluorescent tubes212 h/s 12h (light: dark) illumination.
The cultured algae is Chlorella vulgaris, and is obtained from fresh water algae seed bank of aquatic organism research institute of Chinese academy of sciences, and is numbered as FACHB-8. The control group cultured chlorella in BG11 medium in a volume of 1L. Adding methanol with the volume ratio of 0.5 percent into the culture medium of the methanol experimental group; adding polyethylene glycol dimethyl ether into a culture medium of the NHD experimental group, wherein the final concentration is 5 mmol/L; adding propylene carbonate into a culture medium of a PC experimental group, wherein the final concentration is 5 mmol/L; n-methyl pyrrolidone is added into the culture medium of the NMP experimental group, and the final concentration is 5 mmol/L. Controlling the culture temperature at 25 deg.C, continuously introducing air for 24 hr to drive the mixture of culture solution, the air flow rate is 180mL/min, and adding pure CO into the introduced air every 4 hr within 12 hr of illumination2Gas is once for 10min each time, pure CO2The flow rate of (2) was 20mL/min (pure CO at this time)2Form CO with the introduced air2Mixed gas of 10% by volume).
The initial inoculation concentration of the microalgae is 0.1g/L, the growth condition of algae cells is sampled and measured every day in the culture process, the carbon fixing rate is calculated, the total inorganic carbon source concentration in the culture solution, the photosynthetic oxygen release rate of the algae cells and the activity of extracellular carbonic anhydrase are sampled and measured on the 4 th day of culture, the microalgae is harvested centrifugally after 8 days of culture, and the change of the total organic carbon concentration in the culture solution before and after the culture is measured.
As shown in FIG. 1, the average concentration of the inorganic carbon source in the culture solution of the control group was 4.26 mmol/L during the culture. The average total inorganic carbon source concentration in the culture solution of the methanol experimental group is 6.46mmol/L, which is increased by 51.6 percent compared with that of a control group; the average total inorganic carbon source concentration in the NHD experimental group culture solution is 6.53 mmol/L, which is improved by 53.3 percent compared with the control group; the average total inorganic carbon source concentration in the culture solution of the PC experimental group is 6.68mmol/L, which is increased by 56.8 percent compared with the control group; the average total inorganic carbon source concentration in the NMP experimental group culture solution is 6.48mmol/L, which is increased by 52.1 percent compared with the control group.
As shown in FIG. 2, the average biological carbon fixation efficiency of the control group cultured in batch for 8 days is 100.65 mg/L/d, the average biological carbon fixation efficiency of the methanol experimental group cultured in batch for 8 days is 146.4mg/L/d, and the average biological carbon fixation efficiency is relatively improved by 45.4% compared with that of the control group; the average biological carbon fixation efficiency of the NHD experimental group cultured for 8 days in batch is 137.25mg/L/d, which is relatively improved by 36.4 percent compared with the control group; the average biological carbon fixation efficiency of the PC experimental group cultured in batch for 8 days is 173.85mg/L/d, which is relatively improved by 72.7 percent compared with the control group; the average biological carbon fixation efficiency of the NMP experimental group cultured for 8 days is 155.55mg/L/d, which is relatively improved by 54.5 percent compared with the control group.
As shown in FIG. 3, after 8 days of batch culture, the total organic carbon source concentration in the culture solution of the methanol experimental group is reduced from 393.48mg/L to 319.62mg/L, which indicates that 18.7% of the strengthening agent is lost in the culture process; the concentration of the total organic carbon source in the culture solution of the NHD experimental group is changed from 770.81mg/L to 773.29 mg/L, and the total organic carbon source is within an error range and has no loss; the total organic carbon source concentration in the culture solution of the PC experimental group is reduced from 392.67mg/L to 351.09mg/L, which indicates that the PC is lost by 10.5 percent in the culture process; the total organic carbon source concentration in the culture solution of the NMP experimental group is reduced from 412.7mg/L to 427.45mg/L, which shows that NMP is not lost during the culture process.
As shown in FIGS. 4 and 5, the net photosynthetic oxygen evolution rate of algal cells cultured in methanol experimental group was 68.1. mu. molO2(mg Chlah)-1The activity of extracellular carbonic anhydrase was 8.6 EU/. mu.g, which was 10.3% and 135.6% higher than the control group, respectivelyPercent; the net photosynthetic oxygen evolution rate of algal cells cultured in NHD experimental group was 93.55. mu. molO2(mg Chla h)-1The activity of the extracellular carbonic anhydrase is 7.3 EU/mug, which is respectively increased by 51.5 percent and 100.0 percent compared with the control group; the net photosynthetic oxygen evolution rate of the algal cells cultured in the PC experimental group was 94.5. mu. molO2(mg Chla h)-1The activity of the extracellular carbonic anhydrase is 9.0 EU/mug, which is improved by 53.0 percent and 146.6 percent respectively compared with the control group; the net photosynthetic oxygen evolution rate of the algal cells cultured in the NMP experimental group was 92.9. mu. molO2(mg Chla h)-1The extracellular carbonic anhydrase activity was 9.1 EU/. mu.g, which was increased by 50.5% and 149.3%, respectively, compared to the control group.
Similar effects can be obtained by adjusting the methanol concentration to 0.05-1.0% and the concentrations of NHD, PC and NMP to 0.5-10 mmol/L.
The experiments show that methanol, NHD, PC and NMP can be used as reinforcers to promote the carbon sequestration efficiency of microalgae organisms.
Example 2
The marine nannochloropsis was cultured indoors using a cylindrical glass photobioreactor, and the algal species was from the freshwater algal bank of aquatic organisms institute of academy of sciences of china, and was numbered 926. The control group was cultured in f/2 medium, and the experimental group was cultured in f/2 medium supplemented with PC at a final concentration of 5 mmol/L. Otherwise, as in example 1, the inoculation concentration of the microalgae cells was 0.05 g/L.
During the culture period, the average total inorganic carbon source concentration of the culture solution of the control group is 4.95 mmol/L. The average total inorganic carbon source concentration in the culture solution of the methanol experimental group is 6.46mmol/L, which is improved by 62.2 percent compared with the control group. The average biological carbon fixation efficiency of the control group cultured in batch for 8 days is 121.2mg/L/d, the average biological carbon fixation efficiency of the experimental group cultured in batch for 8 days is 177.8mg/L/d, and the relative improvement is 46.7 percent compared with the control group. After 8 days of batch culture, the concentration of the total organic carbon source in the culture solution of the experimental group is reduced from 430.1 mg/L to 433.9mg/L, which indicates that almost no strengthening agent is lost during the culture process.
Example 3
Open raceway pond culture experiments were performed. The perimeter of the runway pool is 70 meters, and the width of the runway pool is 3 meters. The algae species areScenedesmus dichotoma, which is from freshwater algae seed bank of aquatic creature of academy of sciences of China, and is numbered 496. Culturing Scenedesmus obliquus with BG11 culture medium, wherein the depth of the culture solution is 20 cm; adding PC to a final concentration of 5mmol/L when preparing a culture medium; maintaining the temperature of the culture solution not to exceed 30 ℃ by means of natural evaporation of a water body; the flow of the culture solution is driven by a stirring impeller, and the carbon supplement is automatically controlled (relevant control devices and technologies are disclosed in the publications, such as patents CN200410009360.4 and CN 201210138598.1). The control group was a raceway pond culture performed on a medium to which no PC was added. As a result, the average total inorganic carbon concentration in the culture solution of the control group was 3.77mmol/L, and the carbon fixation rate per unit area was 8.5 g/(m)2D) the average total inorganic carbon concentration in the culture solution of the experimental group was 4.83mmol/L, and the carbon fixation rate per unit area was 10.5 g/(m)2D). In addition, the concentration of the total organic carbon in the culture process of the experimental group is detected, and the change is almost not found, which indicates that the enhancer is not lost in the use process.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. The application of the microalgae carbon sequestration enhancer in improving the microalgae carbon sequestration efficiency.
2. The culture medium capable of improving the carbon sequestration efficiency of the microalgae is characterized by comprising a microalgae carbon sequestration enhancer which is one or a combination of methanol, polyethylene glycol dimethyl ether, propylene carbonate or N-methyl pyrrolidone.
3. The culture medium according to claim 2, wherein the medium comprises methanol at a concentration of 0.05-1.0% volume fraction, or dimethyl ether of polyethylene glycol, propylene carbonate or N-methylpyrrolidone at a concentration of 0.5-10mmol/L, or a combination thereof.
4. The culture medium according to claim 2, wherein the pH of the culture medium is 6 to 8.
5. The culture medium of claim 2, comprising a basal medium composition, wherein the basal medium is a medium for culturing photosynthetic microalgae.
6. The culture medium according to claim 5, wherein the basal medium is BG11 medium, SE medium, or f/2 medium.
7. A method for improving carbon sequestration efficiency of microalgae, comprising a step of culturing the microalgae using the culture medium of any one of claims 2 to 7.
8. The method according to claim 7, wherein the microalgae is cultured by introducing CO into the culture medium2The gas of (2).
9. The method of claim 8, wherein the gas containing CO is introduced into the reaction vessel2The gas of (a) is flue gas, industrial CO2 gas, or air mixed with CO2, or a combination thereof.
10. The method of any one of claims 7 to 9, wherein the microalgae are green algae, diatoms, blue algae, gold algae.
CN202010815304.9A 2020-08-12 2020-08-12 Application of microalgae carbon sequestration enhancer and culture medium containing enhancer Pending CN111925942A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724637A (en) * 2004-07-21 2006-01-25 中国科学院过程工程研究所 Mend the method that carbon is cultivated little algae by pH value feedback control

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1724637A (en) * 2004-07-21 2006-01-25 中国科学院过程工程研究所 Mend the method that carbon is cultivated little algae by pH value feedback control

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ADI NATH ET AL.: "Evaluation of carbon capture in competent microalgal consortium for enhanced biomass,lipid,and carbohydrate production", 《3 BIOTECH》, pages 1 - 15 *
孙中亮: "低浓度二氧化碳培养微藻的吸收强化和烟道气组分调变", 《中国博士学位论文全文数据库基础科学辑》, pages 1 - 3 *
梁梁等: "小球藻固定CO2能力与油脂积累条件研究", 《河南科技》, pages 131 - 135 *

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