CN111909866B - Ferment for VOC gas decomposition and preparation method and application thereof - Google Patents

Ferment for VOC gas decomposition and preparation method and application thereof Download PDF

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CN111909866B
CN111909866B CN202010704626.6A CN202010704626A CN111909866B CN 111909866 B CN111909866 B CN 111909866B CN 202010704626 A CN202010704626 A CN 202010704626A CN 111909866 B CN111909866 B CN 111909866B
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ferment
voc gas
bacillus subtilis
saccharomyces cerevisiae
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CN111909866A (en
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黄俊文
林坤志
邓良敦
邓武敦
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Kunshan Liqing Biotechnology Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/38Removing components of undefined structure
    • B01D53/44Organic components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/34Chemical or biological purification of waste gases
    • B01D53/74General processes for purification of waste gases; Apparatus or devices specially adapted therefor
    • B01D53/84Biological processes
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01D2251/00Reactants
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2257/00Components to be removed
    • B01D2257/70Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
    • B01D2257/708Volatile organic compounds V.O.C.'s
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D2259/00Type of treatment
    • B01D2259/45Gas separation or purification devices adapted for specific applications
    • B01D2259/4508Gas separation or purification devices adapted for specific applications for cleaning air in buildings
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

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Abstract

The invention discloses an enzyme for decomposing VOC gas and a preparation method and application thereof. The ferment comprises a strain composition, wherein the strain composition comprises potato scab bacteria, cheese lactobacillus, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium, and the weight parts of the components are as follows: 20 parts of potato scab bacteria, 10 parts of lactobacillus casei, 20 parts of acetobacter xylinum, 20 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 10 parts of bifidobacterium. The invention combines the unique characteristics of aerobic, facultative and anaerobic floras, can exert the effect in the severe environments of aerobic, anaerobic, acid, alkali and the like, utilizes the floras after the intensive domestication to decompose the generated VOC gas, can quickly and effectively eliminate harmful gas and treat the air.

Description

Ferment for VOC gas decomposition and preparation method and application thereof
Technical Field
The invention relates to an enzyme for decomposing VOC (volatile organic compound) gas as well as a preparation method and application thereof.
Background
With the continuous development of the home decoration market, various problems in home decoration are followed. Especially indoor environmental pollution, has attracted more and more attention due to its harmfulness to human bodies. The VOC, the source of indoor environmental pollution, also gradually floats out of the water. VOCs are organic (carbon-based) gases, also known as hydrocarbons, emitted by many different products. Common sources of volatile organic compounds in the room are decorative building materials, carpets, printers, furniture, paint thinners, glues, cosmetics and certain sprays, and also plastic products.
The main components of VOC are hydrocarbons, halogenated hydrocarbons, oxygen hydrocarbons and nitrogen hydrocarbons, which comprise benzene series, organic chloride, Freon series, organic ketone, amine, alcohol, ether, ester, acid, petroleum hydrocarbon compounds and the like. VOC is the abbreviation of volatile organic compounds, and the chemical substances (except heavy metals) harmful to human bodies in the wall paint are VOC. These volatile organic compounds include formaldehyde, ammonia, ethylene glycol, esters, and the like. When the VOC in a room reaches a certain concentration, symptoms such as headache, nausea, vomiting, hypodynamia and the like can be caused, and even convulsion and coma can be caused in severe cases, so that the liver, the kidney, the brain and the nervous system are injured, and serious consequences such as hypomnesis and the like are caused.
The existing VOC treatment method mainly comprises an active adsorption method, an induced air high-altitude discharge method and a combustion treatment method. 1. An active adsorption method: in the organic waste gas treatment process, the adsorption is one of the methods with good treatment effect and wider application, and the adsorbents comprise active carbon, diatomite, zeolite and the like, wherein the active carbon adsorption is most applied. Through adsorption system, not only can make VOC concentration greatly reduced, realize that waste gas discharge to reach standard, through gaseous desorption, the collection can be used for production again after adsorbing moreover. However, the method has the disadvantages of long treatment time, high cost, incomplete treatment and residual VOC. 2. An induced air high-altitude discharge method: the method is one of the most convenient methods used by common enterprises in the post of paint filling, sanding and the like, and has the advantages of low cost, easy operation and obvious effect. However, the high-altitude discharge is only pollution transfer, the pollution problem is not really solved, and the power and the air inlet installation height of the induced draft fan directly influence the induced draft effect. 3. A combustion treatment method: VOC is organic volatile substance, is easy to burn, can be treated by normal temperature or catalytic oxidation combustion, and gas is introduced into a boiler or an incinerator for combustion through a draft pipeline, but the high-temperature organic gas is subject to safety demonstration.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an enzyme for decomposing VOC gas, a preparation method and application thereof.
In order to solve the technical problems, the invention provides a ferment for decomposing VOC gas, which comprises a strain composition, wherein the strain composition is prepared from potato scab (Streptomyces turkiscaibes), Lactobacillus casei (Lactobacillus casei), Acetobacter xylinum (Acetobacter xylinum), Saccharomyces cerevisiae (Saccharomyces cerevisiae), Bacillus subtilis (Bacillus subtilis) and Bifidobacterium (Bifidobacterium bifidum), and the weight parts of the components are as follows: 20 parts of potato scab bacteria, 10 parts of lactobacillus casei, 20 parts of acetobacter xylinum, 20 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 10 parts of bifidobacterium.
Preferably, the additive also comprises molasses accounting for 10-30% of the total weight.
Preferably, the trace element-containing copper-magnesium alloy further comprises one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, wherein the content of each trace element is one thousandth of the total weight.
Preferably, the mineral composition also comprises minerals, wherein the minerals comprise one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium and sulfur, and the content of each mineral is one thousandth of the total weight.
The invention also provides a preparation method of the ferment for decomposing the VOC gas, which comprises the following steps:
preparing a culture medium stock solution, extracting a part of the stock solution to a new barrel, adding molasses and water into the new barrel, and uniformly stirring;
pouring any five of potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium strains into a glass bottle respectively, adding water, and uniformly shaking until bubbles are generated;
taking out the five strains from each glass bottle, putting the parts into a container, uniformly shaking until bubbles are generated, standing, and pouring into the new barrel;
pouring 25ML of potato scab germs, 15ML of saccharomyces cerevisiae and 15ML of bifidobacteria into the new barrel, and uniformly stirring and aerating to complete a new barrel of culture medium;
pouring the cheese lactobacillus 10ML, the acetobacter xylinum 10ML and the bacillus subtilis 10ML into the new barrel of culture medium again, and uniformly stirring and aerating to obtain the finished enzyme product.
Preferably, the preparation method of the culture medium stock solution comprises the following steps:
Taking 40-60% of water and 10-30% of molasses according to the weight percentage, and then uniformly stirring;
taking 10 strains of potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae and bacillus subtilis, adding water into each strain, adjusting the water content to 4-6% of the total weight, and adding the water;
and aeration observation is carried out to form a culture medium stock solution.
Preferably, the stock solution comprises 50% of the total weight of the new keg.
Preferably, molasses accounting for 10-30% of the total weight and water accounting for 20-40% of the total weight are added into the new barrel.
Preferably, 40-60 ml of any five strains are taken and poured into a glass bottle respectively, and then 400-600 ml of water is added and then the mixture is uniformly shaken.
The invention also provides application of the ferment, wherein the ferment is filled into atomization equipment, and the ferment is sprayed into the space containing the VOC gas to decompose the VOC gas.
The invention achieves the following beneficial effects: the invention combines the unique characteristics of aerobic, facultative and anaerobic floras, can exert the effect in the severe environments of aerobic, anaerobic, acid, alkali and the like, utilizes the floras after the intensive domestication to decompose the generated VOC gas, can quickly and effectively eliminate harmful gas and treat the air. In addition, the potato scab bacteria can decompose organic matters and secrete antibiotic substances, and has the effect of inhibiting diseases, and the bacillus subtilis can inhibit viruses and strengthen the immune function, and has the function of enhancing the immunity of a human body after being inhaled by the human body.
Detailed Description
The present invention will be further described with reference to the following examples. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
The invention provides a ferment for decomposing VOC (volatile organic compounds) gas, which comprises a strain composition, wherein the strain composition comprises potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium, and the weight parts of the components are as follows: 20 parts of potato scab bacteria, 10 parts of lactobacillus casei, 20 parts of acetobacter xylinum, 20 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 10 parts of bifidobacterium.
The invention also comprises molasses with the total weight of 10-30%, and trace elements and minerals.
The trace elements comprise one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, and the content of each trace element is one thousandth of the total weight.
The mineral substances comprise one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium and sulfur, and the content of each mineral substance is one thousandth of the total weight.
The invention provides a preparation method of ferment for decomposing VOC (volatile organic compound) gas, which comprises the following steps of:
(1) Mixing 40-60% of water and 10-30% of molasses according to the weight percentage, and then uniformly stirring;
(2) taking 10 strains of potato scab bacteria, cheese lactic acid bacteria, wood acetic acid bacteria, saccharomyces cerevisiae and bacillus subtilis, adding water into each strain, adjusting the water content to 4-6% of the total weight, and adding the water;
(3) aeration observation is carried out to form a culture medium stock solution;
(4) pre-mixing a culture medium stock solution, and extracting part of the stock solution to a new barrel to ensure that the stock solution accounts for 50% of the total weight of the new barrel;
(5) adding molasses accounting for 10-30% of the total weight and water accounting for 20-40% of the total weight into the new barrel, and uniformly stirring;
(6) taking 40-60 ml of each of five strains from potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium strains, pouring the five strains into a glass bottle respectively, adding 400-600 ml of water, and uniformly shaking until bubbles are generated;
(7) taking 500ml of the five strains out of each glass bottle, putting the five strains into a container, uniformly shaking the five strains until bubbles are generated, standing the five strains for a proper time, and pouring the five strains into a new barrel;
(8) pouring 100-300 ml of potato scab bacteria, saccharomyces cerevisiae and wood acetic acid bacteria into the new barrel, and uniformly stirring and aerating to complete the new barrel culture medium;
(9) And pouring 100-300 ml of lactobacillus casei, bacillus subtilis and bifidobacterium into the new barrel culture medium, uniformly stirring and aerating to finish a finished enzyme product, and adding trace elements and mineral substances into the finished enzyme product.
The invention provides an application of ferment for decomposing VOC gas, the ferment is loaded into atomization equipment, the ferment is sprayed into a space containing the VOC gas, the VOC gas is decomposed, and the decomposition rate of the VOC gas is shown in table 1 after detection:
TABLE 1 decomposition rates of different VOC gases
Detecting gas Decomposition rate Duration of test
Formaldehyde (I) 89.2% 24h
Toluene 89.8% 24h
Xylene 91.3% 24h
Ethyl acetate 92.9% 24h
Hydrogen sulfide 87.0% 24h
Ammonia gas 90.5% 24h
The case of the enzyme used in the decomposition of the VOC gas according to the present invention is shown in table 2:
TABLE 2 case of enzyme treatment of VOC gas
Figure BDA0002594239470000041
Figure BDA0002594239470000051
The invention combines the unique characteristics of aerobic, facultative and anaerobic floras, can exert the effect in the severe environment of aerobic, anaerobic, acid, alkali and the like, utilizes the floras after the intensive domestication to decompose the generated VOC gas, has the decomposition rate of more than 80 percent, and can quickly and effectively eliminate harmful gas and treat air.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims (2)

1. The ferment for decomposing the VOC gas is characterized by comprising a strain composition, wherein the strain composition comprises potato scab bacteria, lactobacillus casei, acetobacter xylinum, saccharomyces cerevisiae, bacillus subtilis and bifidobacterium, and the weight parts of the components are as follows: 20 parts of potato scab bacteria, 10 parts of lactobacillus casei, 20 parts of acetobacter xylinum, 20 parts of saccharomyces cerevisiae, 10 parts of bacillus subtilis and 10 parts of bifidobacterium; the molasses accounts for 10-30% of the total weight; the trace elements comprise one or more of copper, magnesium, zinc, iron, boron, calcium and manganese, and the content of each trace element is one thousandth of the total weight; and the mineral composition also comprises minerals, wherein the minerals comprise one or more of calcium, chlorine, magnesium, phosphorus, potassium, sodium and sulfur, and the content of each mineral is one thousandth of the total weight.
2. The use of the ferment of claim 1, wherein the ferment is loaded into an atomization device, and the VOC gas is decomposed by spraying the ferment into a space containing the VOC gas.
CN202010704626.6A 2020-07-21 2020-07-21 Ferment for VOC gas decomposition and preparation method and application thereof Active CN111909866B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101974430A (en) * 2010-09-30 2011-02-16 北京意科乐生态科技有限公司 Environmentally-friendly microorganism preparation for eliminating house-fitment pollution and preparation method thereof
KR20130134914A (en) * 2012-05-31 2013-12-10 (주) 에코텍씨드 Complex strain for purifing water and improving barn environment and its making method
TW201619372A (en) * 2014-11-25 2016-06-01 黃俊文 Culture medium's replication method
CN106345273A (en) * 2016-08-23 2017-01-25 大工(青岛)新能源材料技术研究院有限公司 Microorganism formaldehyde scavenging agent containing chitosan and preparation method thereof
CN107354193A (en) * 2016-05-09 2017-11-17 黄俊文 The clone method of culture medium
CN108164111A (en) * 2018-01-16 2018-06-15 上海祈舞环保科技有限公司 A kind of biological enzyme purification ferment and its application

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Publication number Priority date Publication date Assignee Title
CN101974430A (en) * 2010-09-30 2011-02-16 北京意科乐生态科技有限公司 Environmentally-friendly microorganism preparation for eliminating house-fitment pollution and preparation method thereof
KR20130134914A (en) * 2012-05-31 2013-12-10 (주) 에코텍씨드 Complex strain for purifing water and improving barn environment and its making method
TW201619372A (en) * 2014-11-25 2016-06-01 黃俊文 Culture medium's replication method
CN107354193A (en) * 2016-05-09 2017-11-17 黄俊文 The clone method of culture medium
CN106345273A (en) * 2016-08-23 2017-01-25 大工(青岛)新能源材料技术研究院有限公司 Microorganism formaldehyde scavenging agent containing chitosan and preparation method thereof
CN108164111A (en) * 2018-01-16 2018-06-15 上海祈舞环保科技有限公司 A kind of biological enzyme purification ferment and its application

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