CN111879759A - Method for rapidly detecting cannabis in drugs on site by utilizing colorimetric reagent - Google Patents
Method for rapidly detecting cannabis in drugs on site by utilizing colorimetric reagent Download PDFInfo
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- CN111879759A CN111879759A CN202010504013.8A CN202010504013A CN111879759A CN 111879759 A CN111879759 A CN 111879759A CN 202010504013 A CN202010504013 A CN 202010504013A CN 111879759 A CN111879759 A CN 111879759A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/7756—Sensor type
- G01N2021/7759—Dipstick; Test strip
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Abstract
The invention provides a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which is prepared from 2, 6-dichloroquinone-4-chloroimine, water and sodium hydroxide, wherein test paper is common filter paper, a cotton swab is used for collecting a sample, the sample is placed on the test paper, the colorimetric reagent is dripped on the test paper containing the sample, and the existence of cannabis is determined by observing whether a grayish green compound is generated or not. The detection method can be used for field drug analysis and qualitative detection of cannabis drugs in drug-involved cases, and the detection limit can reach 10 mu g. The method does not need to dissolve suspicious samples, has the characteristics of simple and rapid operation, strong specificity, sensitive reaction, convenient use and low cost, and can provide an effective technical means for the detection of drug-involved cases in the criminal investigation department of the public security system.
Description
Technical Field
The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, in particular to cannabis, cannabis extract or other cannabis products.
Background
In recent years, the development of a rapid, sensitive and accurate field drug detection technology has very important significance. At present, the commercialized field drug detection means comprise ion mobility spectrometry, Raman spectroscopy, olfactory dog, fluorescence method and the like, but from the aspects of sensitivity and portability, the ion mobility spectrometry has the defects of time consumption in calibration and higher cost; portable raman techniques generally require large sample volumes and expensive instruments; the olfactory poisoning dog is also influenced by factors such as emotion and the like, and is difficult to continuously and stably detect.
Cannabis is a common drug with complex components, and tetrahydrocannabinol in cannabis is believed to increase the release of nucleus accumbens dopamine, resulting in a potentiating effect. Cannabinoids have also been found to interact with a number of neurotransmitters, including gamma-aminobutyric acid, glutamic acid, choline and the like. Cannabis mainly acts on the human body through cannabinoid receptors, which are G protein-coupled receptors and can inhibit the activity of adenylate cyclase through inhibitory G proteins, thereby lowering intracellular cyclic adenosine levels, and further, cannabinoid receptors can regulate ion channels, causing a decrease in neurotransmitter release in presynaptic membrane neurons, thereby causing corresponding excitatory or inhibitory effects on postsynaptic membranes. The main hazards are: (1) the cannabinol effect causes tachycardia, the heart rate is increased by 20-50 percent, and the maximum can reach 140 and 150 times/minute; (2) the long-term use causes the damage of the functions of the lung and the upper respiratory tract, and can cause diseases such as tracheitis, pharyngitis, edema of the larynx and the like; (3) cannabinol can impair cellular regulation and humoral regulation, reducing the ability to resist bacterial and viral infections; (4) excessive intake of cannabis results in relaxation of muscle tone and dysfunction of the balance; (5) excessive intake of cannabis can cause unconsciousness, anxiety, depression, etc., which are easy to generate hostile impulsion or suicide will; (6) the abuse of marijuana can cause the damage of large-scale cognitive function, and can reduce short-term brain memory, thinking, attention and judgment, and make people slow in thinking, pina and disordered memory. Because cannabis is easily available and exists in part of national regions even legally, detection and control of cannabis is an important link in handling drug-related cases.
At present, the cannabis detection methods mainly comprise two types, one type is azo salt dyes, and the azo salt dyes can generate condensation reaction with phenolic substances in cannabis under an acidic or alkaline environment to generate color change. The method utilizes diazotization reaction, but the specificity depends on the formation of conjugated molecules, and amine substances with similar structures can also react with the conjugated molecules and cannot be distinguished. And the other method is to qualitatively analyze phenolic substances by utilizing the Folin-Ciocalteu method and utilizing the oxidation reaction of phosphomolybdate-phosphotungstate on phenol. Chinese patent: CN105021608 uses the optimized Folin-Ciocalteu method to detect the total polyphenol content of the beverage. However, the method has long color development time, reacts with all phenols, and is not suitable for on-site rapid detection of cannabis, and the detection result is easy to interfere.
The invention provides a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which mainly utilizes 2, 6-dichloroquinone-4-chloroimine to carry out condensation reaction with tetrahydrocannabinol, the main component of which is phenolic hydroxyl group and is not substituted, in cannabis sativa to generate a grey-green compound to determine the existence of the tetrahydrocannabinol, thereby realizing the detection of cannabis sativa. The colorimetric reagent can perform color reaction with phenolic compounds which are not substituted at the para-position of the phenolic hydroxyl group, but the color can generate different colors according to the structures of the compounds (for example, the colorimetric reagent reacts with phenol derivatives to generate bright blue). The reagent has the advantages of easily obtained raw materials, simple and convenient preparation, light weight, portability, strong selectivity and wide application potential.
Disclosure of Invention
The invention aims to provide a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which is based on a chemical colorimetric method, does not need to dissolve a suspicious sample, and utilizes 2, 6-dichloroquinone-4-chloroimine to react with tetrahydrocannabinol in cannabis to generate a gray-green compound so as to achieve the effect of detecting cannabis. The detection method can be used for field drug analysis and qualitative detection of cannabis drugs in drug-involved cases, has the characteristics of simple and rapid operation, strong specificity, sensitive response, convenient use and low cost, and can provide an effective technical means for detecting drug-involved cases in public security system criminal investigation departments.
The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which comprises the following operation steps:
a. weighing 0.005-0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethanol, fully stirring until the absolute ethanol is completely dissolved, sealing, introducing nitrogen for 20min, and packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. b, extracting a detection sample from the object to be detected by using a cotton swab, wiping the detection sample on test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, and observing color change to determine whether hemp, hemp extract or other hemp products exist in the object to be detected.
The invention relates to a method for rapidly detecting cannabis in drugs on site by utilizing a colorimetric reagent, which comprises the steps of taking the colorimetric reagent prepared from 2, 6-dichloroquinone-4-chloroimine, sodium hydroxide and water as the detection reagent at room temperature, collecting a sample by using a cotton stick, placing the sample on test paper, dropwise adding the detection reagent on the test paper containing the sample, and reacting the 2, 6-dichloroquinone-4-chloroimine with tetrahydrocannabinol in the cannabis to generate a gray-green compound so as to determine the existence of the cannabis; if the color is gray green, the object to be detected is proved to contain the hemp; if the color is not gray green, the object to be tested is proved to contain no cannabis.
The method for rapidly detecting the cannabis in the drugs on site by using the colorimetric reagent can detect at least 10 mu g of cannabis.
Detailed Description
Example 1
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. extracting a detection sample from a substance to be detected, dripping 10 mu L of 1g/L hemp lifting liquid (ethanol as a solvent) onto a test board, dripping the detection reagent A obtained in the step a onto the test board containing the detection sample, dripping the detection reagent B, observing color change, generating color reaction within 1s, and changing into grey green to prove that the sample contains hemp
Example 2
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the substance to be detected, dripping 10 mu L of 1g/L phenol solution (ethanol is used as a solvent) onto the test board, dripping the detection reagent A obtained in the step a onto the test board containing the detection sample, then dripping the detection reagent B, observing color change, generating color reaction within 1s, and changing the color reaction into blue, thereby proving that the sample does not contain the hemp.
Example 3
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the substance to be detected, dripping 10 mu L of 1g/L naphthol solution (ethanol as a solvent) onto a test board, dripping the detection reagent A obtained in the step a onto the test board containing the detection sample, then dripping the detection reagent B, observing color change, generating color reaction within 1s, and changing the color reaction into blue, thereby proving that the sample does not contain hemp.
Example 4
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the object to be detected, dripping 10 mu L of water sample onto the test board, dripping the detection reagent A obtained in the step a onto test paper containing the detection sample, dripping the detection reagent B, observing the color change, generating color reaction within 1s, changing the color reaction into purple, and proving that the water sample does not contain hemp.
Example 5
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from broken hemp leaves to be detected by using a cotton swab, wiping the detection sample onto test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, observing the color change within 1s to generate a grayish green compound, and determining that the sample contains hemp.
Example 6
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from a suspected hemp product to be detected by using a cotton swab, wiping the detection sample onto a test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, then dropwise adding the detection reagent B, observing color change, and generating a grayish green compound within 1s so as to determine that the sample contains hemp.
Example 7
a. Weighing 0.005g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (3) extracting a detection sample from a cannabinol analogue (chemical reagent) to be detected by using a cotton swab, wiping the detection sample on a test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, observing color change, and generating a grayish green compound within 1s so as to determine that the sample contains the cannabis.
Example 8
a. Weighing 0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the hemp extract to be detected by using a dropper, wiping the detection sample on a test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, then dropwise adding the detection reagent B, observing color change, and generating a grayish green compound within 1s so as to determine that the sample contains hemp.
Example 9
a. Weighing 0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the broken hemp leaves to be detected by using a cotton swab, wiping the detection sample onto test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, then dropwise adding the detection reagent B, observing color change, and generating a grayish green compound within 1s so as to determine that the sample contains hemp.
Example 10
a. Weighing 0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from the hemp seed powder to be detected by using a cotton swab, wiping the detection sample onto test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, observing color change, and generating a grayish green compound within 1s so as to determine that the sample contains hemp.
Example 11
a. Weighing 0.008g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethyl alcohol, fully stirring until the absolute ethyl alcohol is completely dissolved, sealing, introducing nitrogen for 20min, and then packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. and (B) extracting a detection sample from industrial hemp (the content of tetrahydrocannabinol is less than 0.3%) to be detected by using a cotton swab, wiping the detection sample on test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, observing color change, and generating a gray-green compound within 1s to determine that the sample contains the hemp.
Claims (1)
1. A method for rapidly detecting cannabis in drugs on site by utilizing colorimetric reagents is characterized by comprising the following operation steps:
a. weighing 0.005-0.01g of 2, 6-dichloroquinone-4-chloroimine at room temperature, adding 20mL of absolute ethanol, fully stirring until the absolute ethanol is completely dissolved, sealing, introducing nitrogen for 20min, and packaging a detection reagent in a reagent bottle to obtain a detection reagent A;
b. weighing 0.04g of sodium hydroxide, adding water, stirring until the sodium hydroxide is fully dissolved, then fixing the volume to a 100mL volumetric flask to obtain 0.01mol/L sodium hydroxide solution, and encapsulating the sodium hydroxide solution in a reagent bottle to obtain a detection reagent B;
c. b, extracting a detection sample from the object to be detected by using a cotton swab, wiping the detection sample on test paper, dropwise adding the detection reagent A obtained in the step a onto the test paper containing the detection sample, dropwise adding the detection reagent B, and observing color change to determine whether hemp, hemp extract or other hemp products exist in the object to be detected.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999054739A1 (en) * | 1998-04-21 | 1999-10-28 | Peter Rausch | Detection and determination of phenol-like substances by means of direct wet-chemical methods |
| WO2012049486A1 (en) * | 2010-10-13 | 2012-04-19 | Sphere Medical Limited | Analyte detection method |
| CN103743858A (en) * | 2013-12-21 | 2014-04-23 | 广西科技大学 | Hemp seed oil detection reagent and application thereof |
| CN107101999A (en) * | 2017-05-18 | 2017-08-29 | 黑龙江省科学院大庆分院 | The method that sxemiquantitative quickly recognizes THC content in cannabis plants |
| CN110146495A (en) * | 2019-05-13 | 2019-08-20 | 青海大学 | A kind of micro explosive and drugs device for fast detecting |
-
2020
- 2020-06-05 CN CN202010504013.8A patent/CN111879759A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999054739A1 (en) * | 1998-04-21 | 1999-10-28 | Peter Rausch | Detection and determination of phenol-like substances by means of direct wet-chemical methods |
| WO2012049486A1 (en) * | 2010-10-13 | 2012-04-19 | Sphere Medical Limited | Analyte detection method |
| CN103743858A (en) * | 2013-12-21 | 2014-04-23 | 广西科技大学 | Hemp seed oil detection reagent and application thereof |
| CN107101999A (en) * | 2017-05-18 | 2017-08-29 | 黑龙江省科学院大庆分院 | The method that sxemiquantitative quickly recognizes THC content in cannabis plants |
| CN110146495A (en) * | 2019-05-13 | 2019-08-20 | 青海大学 | A kind of micro explosive and drugs device for fast detecting |
Non-Patent Citations (1)
| Title |
|---|
| 郭蓉 等: ""四氢大麻酚和大麻二酚的药理研究进展"", 《天然产物研究与开发》 * |
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