CN104316518A - Detection method for formaldehyde in food - Google Patents
Detection method for formaldehyde in food Download PDFInfo
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- CN104316518A CN104316518A CN201410559354.XA CN201410559354A CN104316518A CN 104316518 A CN104316518 A CN 104316518A CN 201410559354 A CN201410559354 A CN 201410559354A CN 104316518 A CN104316518 A CN 104316518A
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Abstract
The invention relates to a detection method for formaldehyde in food. The detection method comprises the following steps: taking an appropriate amount of sample, adding ethyl alcohol, stirring, uniformly mixing through ultrasound manner, filtering, taking a filtrate, dropwise adding a precipitator into the filtrate, filtering, thus obtaining the filtrate which is the to-be-tested sample solution; weighting anhydrous sodium sulfite, adding distilled water, respectively placing into three colorimetric tubes for later use; adding the prepared to-be-tested sample solution and formaldehyde solution respectively into two of the thee colorimetric tubes, wherein the third colorimetric tube is taken as a blank comparison tube; dropwise adding 2-3 drops of phenolphthalein in the three test tubes, observing color change of the solutions, thus judging that whether the sample contains formaldehyde or the prepared to-be-tested sample solution, if the solution changes into amaranth, the sample contains formaldehyde. The detection method is simple and convenient, the detection time is short, the sample is pretreated before detection, and thus the reliability of the detection result is improved.
Description
Technical field
The invention belongs to field of food detection, be specifically related to.
Background technology
Formaldehyde is a kind of colourless, gas of having intense stimulus smell, soluble in water and alcohol ether, and its boiling point is low, when room temperature and volatilizable.Formaldehyde belongs to one of high toxic material, WHO is defined as carcinogenic, teratogenesis material, in addition, formaldehyde all can work the mischief to human respiratory, immune system and internal system etc., chronic respiratory disease, colon cancer, irregular menstruation can be caused, teenager's memory and intelligence can also be caused to decline.Formaldehyde also has freezing action, not easily metabolism and excretion after being combined with human body internal protein, in vivo can leukemogenesis after long term accumulation.
" food additives use hygienic standard " GB2760-2007 appendix C food industry processing aid uses in list, and formaldehyde is listed in the 38th, but be defined in simultaneously make finished product before should remove or strictly should control residual quantity.And wholefood, under the effect of the various factors such as temperature, humidity or light, the reaction such as autoxidation or decomposition can occur, thus produce formaldehyde.In addition, China also has many illegal retailers to extend the shelf life of food, or makes the vivid duty of its surface light, and the normal formaldehyde that adopts processes food.
Therefore, the content of formaldehyde in food is measured, ensure that Safety of Food Quality seems very important.The assay method of formaldehyde has many, spectrophotometric method, vapor-phase chromatography, liquid phase chromatography, polarography etc., but these methods all have certain limitation, as spectrophotometric method, although have easy and simple to handle, be easy to the advantages such as popularization, specificity difference and poor sensitivity; Chromatography selectivity and highly sensitive, but sample preparation is complicated, and test period is long.Existing detection method cannot detect the formaldehyde in beverage fast.
Prior art is compared, and beneficial effect of the present invention is as follows:
1 the present invention utilizes formaldehyde and sodium sulphite reaction generate NaOH and make sample be alkalescence, is index agent at phenol peptide, and whether the principle of sample meeting displaing amaranth measures containing formaldehyde in sample, and detection method is easy, and detection time is short.
2. because formaldehyde in food content is very low, testing result may show false negative, for this reason, the present invention first carries out pre-service to sample before detecting, comprise ultrasonic mixing after adding ethanol, filter, the mixing solution adding zinc acetate and ethanol to remove in sample protein and other materials such as fatty to the interference detected as precipitation agent, improves the true and reliable property of testing result.
3., in the invention of this group, have employed formaldehyde standard solution as positive control, blank group is also set, effectively eliminate the impact of various disturbing factor on this group testing result.
Summary of the invention
Object of the present invention is exactly the Quick qualitative detection method providing a kind of formaldehyde in food for the defect existed in above-mentioned prior art.
Technical scheme of the present invention is as follows:
A detection method for formaldehyde in food, comprises the following steps:
(1) prepare testing sample solution: take solid sample 2-5g, adopt comminutor pulverize or measure fluid sample 5-10ml, add ethanol and stir ultrasonic mixing 5-10min, after filtering, get filtrate and add precipitation agent, again filter, get filtrate and be testing sample solution;
(2) preparation detects solution: take 5-10g anhydrous sodium sulfite, adding distil water 50-100ml dissolves mixing, inserts in 3 color comparison tubes for subsequent use respectively;
(3) testing sample solution of preparation in step (1) is added in 2 color comparison tubes in step (3) respectively, formalin, the 3rd color comparison tube, as blank pipe, drips 2-3 respectively and drips phenolphthalein in three test tubes, observation solution colour changes, thus is also containing formaldehyde in judgement sample.
Preferably, adding the ratio that the sample described in when second stirs and evenly mixs and the weight of ethanol divides in step (1) is 1:(1 ~ 3).
Preferably, the precipitation agent described in step (1) is the mixed solution of zinc acetate and ethanol.
Preferably, described acetic acid zinc concentration is 3%-8%.
Preferably, described zinc acetate and the volume ratio of ethanol are (20-40): (80-60).
Preferably, the amount of the liquid added in 3 color comparison tubes in step (2) is identical, is 10 ~ 15ml.
Preferably, the concentration of the formalin described in step (3) is 10 ~ 200ug/ml.
Preferably, the volume ratio of the liquid in the testing sample solution, formalin and the color comparison tube that add in 2 color comparison tubes in step (3) is (0.5-0.8): (1-1.5).
Add the solution change aubergine in the color comparison tube of sample solution in step (3), change consistent with solution colour in formalin group color comparison tube, and blank group color is unchanged, then contains formaldehyde in judgement sample.
Embodiment
Below in conjunction with embodiment, the present invention will be further described:
Embodiment 1
A detection method for formaldehyde in food, comprises the following steps:
(1) prepare testing sample solution: take solid sample 2g, adopt comminutor to pulverize, add ethanol and stir ultrasonic mixing 5min, after filtering, get filtrate and add the zinc acetate and ethanol that volume ratio is 20:80, again filter, get filtrate and be testing sample solution;
(2) preparation detects solution: take 5g anhydrous sodium sulfite, adding distil water 50ml dissolves mixing, and in 3 color comparison tubes, add 10ml liquid respectively for subsequent use;
(3) testing sample solution of preparation in step (1) is added in 2 color comparison tubes in step (3) respectively, the formalin of 10ug/ml, 3rd color comparison tube is as blank pipe, 2 phenolphthalein are dripped respectively in three test tubes, observation solution colour changes, thus is also containing formaldehyde in judgement sample.
Adding the ratio that the sample described in when second stirs and evenly mixs and the weight of ethanol divides in step (1) is 1:1.
Described acetic acid zinc concentration is 3%.
The volume ratio of the liquid in the testing sample solution, formalin and the color comparison tube that add in 2 color comparison tubes in step (3) is 0.5:1.
Embodiment 2
A detection method for formaldehyde in food, comprises the following steps:
(1) prepare testing sample solution: take solid sample 5g, adopt comminutor to pulverize, add ethanol and stir ultrasonic mixing 10min, after filtering, getting filtrate, to add volume ratio be the zinc acetate of 40:60 and the mixed liquor of ethanol, again filters, get filtrate and be testing sample solution;
(2) preparation detects solution: take 10g anhydrous sodium sulfite, adding distil water 100ml dissolves mixing, and in 3 color comparison tubes, add 15ml liquid respectively for subsequent use;
(3) testing sample solution of preparation in step (1) is added in 2 color comparison tubes in step (3) respectively, the formalin of 200ug/ml, 3rd color comparison tube is as blank pipe, 3 phenolphthalein are dripped respectively in three test tubes, observation solution colour changes, thus is also containing formaldehyde in judgement sample.
Adding the ratio that the sample described in when second stirs and evenly mixs and the weight of ethanol divides in step (1) is 1:3.
Described acetic acid zinc concentration is 8%.
The volume ratio of the liquid in the testing sample solution, formalin and the color comparison tube that add in 2 color comparison tubes in step (3) is 0.8:1.5.
Embodiment 3
A detection method for formaldehyde in food, comprises the following steps:
(1) prepare testing sample solution: measure fluid sample 10ml, add ethanol and stir ultrasonic mixing 10min, after filtering, getting filtrate, to add volume ratio be the zinc acetate of 30:70 and the mixed liquor of ethanol, again filters, get filtrate and be testing sample solution;
(2) preparation detects solution: take 8g anhydrous sodium sulfite, adding distil water 80ml dissolves mixing, and in 3 color comparison tubes, add 12ml liquid respectively for subsequent use;
(3) testing sample solution of preparation in step (1) is added in 2 color comparison tubes in step (3) respectively, the formalin of 100ug/ml, 3rd color comparison tube is as blank pipe, 2 phenolphthalein are dripped respectively in three test tubes, observation solution colour changes, thus is also containing formaldehyde in judgement sample.
Adding the ratio that the sample described in when second stirs and evenly mixs and the weight of ethanol divides in step (1) is 1:2.
Described acetic acid zinc concentration is 5%.
The volume ratio of the liquid in the testing sample solution, formalin and the color comparison tube that add in 2 color comparison tubes in step (3) is 0.6:1.2.
Described embodiment is preferred embodiments of the present invention, is not limit the scope of the present invention, therefore all equivalences done according to structure, feature and the principle described in the present patent application the scope of the claims change or modify, and all should comprise in patent claim of the present invention.
Claims (8)
1. a detection method for formaldehyde in food, is characterized in that, comprises the following steps:
(1) prepare testing sample solution: take solid sample 2-5g, adopt comminutor pulverize or measure fluid sample 5-10ml, add ethanol and stir ultrasonic mixing 5-10min, after filtering, get filtrate and add precipitation agent, again filter, get filtrate and be testing sample solution;
(2) preparation detects solution: take 5-10g anhydrous sodium sulfite, adding distil water 50-100ml dissolves mixing, inserts in 3 color comparison tubes for subsequent use respectively;
(3) testing sample solution of preparation in step (1) is added in 2 color comparison tubes in step (3) respectively, formalin, the 3rd color comparison tube, as blank pipe, drips 2-3 respectively and drips phenolphthalein in three test tubes, observation solution colour changes, thus is also containing formaldehyde in judgement sample.
2. the detection method of a kind of formaldehyde in food according to claim 1, is characterized in that, it is characterized in that, adding the ratio that the sample described in when second stirs and evenly mixs and the weight of ethanol divides in step (1) is 1:(1 ~ 3).
3. the detection method of a kind of formaldehyde in food according to claim 1, is characterized in that, the precipitation agent described in step (1) is the mixed solution of zinc acetate and ethanol.
4. the detection method of a kind of formaldehyde in food according to claim 2, is characterized in that, described acetic acid zinc concentration is 3%-8%.
5. the detection method of a kind of formaldehyde in food according to claim 2, is characterized in that, described zinc acetate and the volume ratio of ethanol are (20-40): (80-60).
6. the detection method of a kind of formaldehyde in food according to claim 1, is characterized in that, the amount of the liquid added in 3 color comparison tubes in step (2) is identical, is 10 ~ 15ml.
7. the detection method of a kind of formaldehyde in food according to claim 1, is characterized in that, the concentration of the formalin described in step (3) is 10 ~ 200ug/ml.
8. the detection method of a kind of formaldehyde in food according to claim 1, it is characterized in that, the volume ratio of the liquid in the testing sample solution, formalin and the color comparison tube that add in 2 color comparison tubes in step (3) is (0.5-0.8): (1-1.5).
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| CN201410559354.XA CN104316518A (en) | 2014-10-18 | 2014-10-18 | Detection method for formaldehyde in food |
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| CN201410559354.XA CN104316518A (en) | 2014-10-18 | 2014-10-18 | Detection method for formaldehyde in food |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823774A (en) * | 2016-03-22 | 2016-08-03 | 河北安捷仑生物科技有限公司 | Rapid detection method for formaldehyde in food |
| CN106841192A (en) * | 2017-04-18 | 2017-06-13 | 乐凯（沈阳）科技产业有限责任公司 | A kind of diazepine structure directing agent detects carbaldehyde Method and its application |
| CN107014762A (en) * | 2017-03-03 | 2017-08-04 | 苍南县质量技术监督检测院 | A kind of quick determination method of formaldehyde |
| CN107389579A (en) * | 2017-07-11 | 2017-11-24 | 安徽宏远职业卫生技术服务有限公司 | A kind of method of meat food sample pretreatment during detection formaldehyde |
| CN109374612A (en) * | 2018-11-14 | 2019-02-22 | 遵义市精科信检测有限公司 | A kind of detection method of formaldehyde in food |
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| CN1731156A (en) * | 2005-08-19 | 2006-02-08 | 中国检验检疫科学研究院 | Method for quick detection of formaldehyde in food |
| CN103616380A (en) * | 2013-12-03 | 2014-03-05 | 广东中测食品化妆品安全评价中心有限公司 | Reagent and method for rapidly detecting formaldehyde in foods |
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| CN1731156A (en) * | 2005-08-19 | 2006-02-08 | 中国检验检疫科学研究院 | Method for quick detection of formaldehyde in food |
| CN103616380A (en) * | 2013-12-03 | 2014-03-05 | 广东中测食品化妆品安全评价中心有限公司 | Reagent and method for rapidly detecting formaldehyde in foods |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823774A (en) * | 2016-03-22 | 2016-08-03 | 河北安捷仑生物科技有限公司 | Rapid detection method for formaldehyde in food |
| CN105823774B (en) * | 2016-03-22 | 2018-12-21 | 河北安捷仑生物科技有限公司 | Formaldehyde in food rapid detection method |
| CN107014762A (en) * | 2017-03-03 | 2017-08-04 | 苍南县质量技术监督检测院 | A kind of quick determination method of formaldehyde |
| CN106841192A (en) * | 2017-04-18 | 2017-06-13 | 乐凯（沈阳）科技产业有限责任公司 | A kind of diazepine structure directing agent detects carbaldehyde Method and its application |
| CN106841192B (en) * | 2017-04-18 | 2019-05-03 | 乐凯（沈阳）科技产业有限责任公司 | A kind of diazepine structure directing agent detection carbaldehyde Method and its application |
| CN107389579A (en) * | 2017-07-11 | 2017-11-24 | 安徽宏远职业卫生技术服务有限公司 | A kind of method of meat food sample pretreatment during detection formaldehyde |
| CN109374612A (en) * | 2018-11-14 | 2019-02-22 | 遵义市精科信检测有限公司 | A kind of detection method of formaldehyde in food |
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Application publication date: 20150128 |