CN111879739A - Test light source system of microbial luminescence tester and detection method thereof - Google Patents
Test light source system of microbial luminescence tester and detection method thereof Download PDFInfo
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- CN111879739A CN111879739A CN202010640456.XA CN202010640456A CN111879739A CN 111879739 A CN111879739 A CN 111879739A CN 202010640456 A CN202010640456 A CN 202010640456A CN 111879739 A CN111879739 A CN 111879739A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 43
- 238000012360 testing method Methods 0.000 title claims abstract description 38
- 238000004020 luminiscence type Methods 0.000 title claims abstract description 36
- 238000001514 detection method Methods 0.000 title abstract description 15
- 244000005700 microbiome Species 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 18
- 238000002360 preparation method Methods 0.000 abstract description 2
- 230000000087 stabilizing effect Effects 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/062—LED's
Abstract
The invention discloses a test light source system of a microbial luminescence tester and a detection method thereof, wherein the system comprises: the lower surface of the shell is provided with a light hole; a controller disposed inside the housing; the sliding end of the first adjustable resistance module is connected with the controller; the control end of the second adjustable resistance module is connected with the controller, and the first end of the second adjustable resistance module is connected with the first end of the first adjustable resistance module; the negative pole of the LED lamp is connected with the second end of the first adjustable resistance module, and the LED lamp is arranged in the shell and just opposite to the position of the light-transmitting hole. The invention can complete the performance detection process of the microbial luminescence tester without testing reagents, saves the reagents and avoids errors caused by artificial reagent preparation. The invention can be widely applied to the technical field of microorganism testing.
Description
Technical Field
The invention relates to the technical field of microorganism testing, in particular to a testing light source system of a microorganism luminescence tester and a detection method thereof.
Background
The microbial luminous tester is used for detecting the luminous intensity of certain microbes. Before the microbial luminescence tester is used for detecting the luminescence intensity of microorganisms, the performance of the microbial luminescence tester needs to be tested. In the prior art, the testing performance of the microbial luminescent testing light source is generally checked and calibrated by preparing a microbial testing reagent in a corresponding proportion. However, this type of inspection and calibration is wasteful of test reagents, and is prone to large test errors due to manual dispensing of reagents.
Disclosure of Invention
To solve one of the above technical problems, the present invention aims to: the test light source system of the microbial luminescence tester and the detection method thereof are provided, and a test reagent is not needed, so that the reagent is saved, and errors caused by manual reagent preparation are avoided.
A first aspect of an embodiment of the present invention provides:
a test light source system of a microbial luminescence tester, comprising:
the lower surface of the shell is provided with a light hole;
a controller disposed inside the housing;
the sliding end of the first adjustable resistance module is connected with the controller;
the control end of the second adjustable resistance module is connected with the controller, the first end of the second adjustable resistance module is connected with the first end of the first adjustable resistance module, and the second end of the second adjustable resistance module is grounded;
the cathode of the LED lamp is connected with the second end of the first adjustable resistor module, and the LED lamp is arranged in the shell and is opposite to the position of the light hole;
and the power supply module is connected with the anode of the LED lamp.
Furthermore, the system also comprises a sliding block, a driving module and a motor, wherein the input end of the driving module is connected with the controller, the output end of the driving module is connected with the motor, and the motor is used for controlling the sliding position of the sliding block.
Furthermore, the lower end of the shell is provided with a sliding groove, and the sliding block slides in the sliding groove.
Furthermore, the slider is in a rectangular shape with a fan-shaped notch in the middle.
Further, the second adjustable resistor comprises a multiplexer and a plurality of resistors, the control end of the multiplexer is connected with the controller, the output end of the multiplexer is connected with the first end of the first adjustable resistor module, the switch ends of the multiplexer are respectively connected with the first ends of the resistors, and the second ends of the resistors are all grounded.
Furthermore, the system also comprises a voltage stabilizing module, wherein the input end of the voltage stabilizing module is connected with the power supply module, and the output end of the voltage stabilizing module is connected with the anode of the LED lamp.
A second aspect of an embodiment of the present invention provides:
a detection method of a microbial luminous tester detects through the test light source system, and comprises the following steps:
obtaining the type of a target microorganism;
setting the working state of a second adjustable resistance module according to the type of the target microorganism;
adjusting the working state of the first adjustable resistance module;
when the working state of the first adjustable resistance module is adjusted, reading the relative light intensity displayed by the microbial luminescence tester;
and analyzing the working performance of the microbial luminescence tester according to the relative light intensity.
Further, after reading the relative light intensity displayed by the microbial luminescence tester, the method further comprises the following steps:
and adjusting the working state of the second adjustable resistance module.
Further, the adjusting the working state of the first adjustable resistance module specifically includes:
and controlling the position of the sliding end of the first adjustable resistance module through a controller.
Further, the setting of the working state of the second adjustable resistance module specifically includes:
and selecting the working resistance of the second adjustable resistance module through a controller.
The embodiment of the invention has the beneficial effects that: according to the embodiment of the invention, the controller, the first adjustable resistance module, the second adjustable resistance module and the LED lamp are arranged, and the luminous intensity of the LED lamp is controlled by controlling the resistance of the first adjustable resistance module and the second adjustable resistance module which participate in the work, so that the luminous intensity of the LED lamp is close to that of the microorganism, the performance detection process of the microorganism luminous tester can be completed without testing reagents, the reagents are saved, and errors caused by manual reagent blending are avoided.
Drawings
FIG. 1 is a block diagram of a testing light source system of a microbial luminescence tester according to an embodiment of the present invention;
FIG. 2 is a schematic view of the whole test light source system of the microbial luminous tester according to an embodiment of the present invention;
FIG. 3 is a cross-sectional view of a test light source system of the microbial luminescence tester according to an embodiment of the present invention;
FIG. 4 is a schematic view of the slider position of the first embodiment;
FIG. 5 is a schematic view of the slider position of the second embodiment;
FIG. 6 is a schematic view of the slider position of the third embodiment;
FIG. 7 is a flow chart of a method for testing the bioluminescent test instrument according to one embodiment of the present invention.
Detailed Description
The invention is described in further detail below with reference to the figures and the specific embodiments. The step numbers in the following embodiments are provided only for convenience of illustration, the order between the steps is not limited at all, and the execution order of each step in the embodiments can be adapted according to the understanding of those skilled in the art.
In the following description, reference is made to "some embodiments" which describe a subset of all possible embodiments, but it is understood that "some embodiments" may be the same subset or different subsets of all possible embodiments, and may be combined with each other without conflict.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing embodiments of the present application only and is not intended to be limiting of the application.
Referring to fig. 1, an embodiment of the present invention provides a test light source system of a microbial luminescence tester, and this embodiment may be applied to a performance analysis process of the microbial luminescence tester, where the test light source system of this embodiment is placed on a microbial reagent placing table of the microbial luminescence tester, so as to simulate a luminescence intensity of a microbial reagent by the test light source system of this embodiment, thereby completing a performance calibration process of the microbial luminescence tester.
Specifically, as shown in fig. 1, 2, and 3, the present embodiment includes:
the housing 200, the lower surface of the housing 200 is provided with a light hole 210, and in some embodiments, since the photomultiplier tube in the microbial light-emitting tester obtains the light source generated by the microorganism through a fixed position, the light hole 210 is provided with a cylindrical light guide hole to facilitate the photomultiplier tube to obtain the light source.
A controller disposed in the housing 200, specifically on the PCB 270 in the housing 200.
The sliding end of the first adjustable resistance module is connected with the controller; in some embodiments, to facilitate the controller to control the magnitude of the resistance value on the first adjustable resistance module, a digital resistor may be used in place of the first adjustable resistance module. The clock signal and the data signal of the digital resistor are connected with the controller to receive the control signal sent by the controller. Because the resistance value variation range of the digital resistor is small, the weak current adjusting process of the LED lamp is realized by adjusting the resistance value of the digital resistor.
The control end of the second adjustable resistance module is connected with the controller, the first end of the second adjustable resistance module is connected with the first end of the first adjustable resistance module, and the second end of the second adjustable resistance module is grounded;
in some embodiments, the second adjustable resistor includes a multiplexer and a plurality of resistors R1 to R6, a control terminal of the multiplexer is connected to the controller, an output terminal of the multiplexer is connected to the first terminal of the first adjustable resistor module, a plurality of switch terminals of the multiplexer are respectively connected to the first terminals of the plurality of resistors, and second terminals of the plurality of resistors are all grounded. In this embodiment, the resistance values of the resistors R1 to R6 are set, the resistance difference between the resistors R1 to R6 is large, and then the controller outputs a control signal to control the multiplexer to select one or more resistors to operate, so that the resistance value is changed to a large extent to control the current of the LED lamp to a large extent.
The cathode of the LED lamp 280 is connected with the second end of the first adjustable resistor module, and the LED lamp is arranged in the shell and is opposite to the position of the light hole 210, so that a photomultiplier in the microbial luminescence tester can obtain a light source conveniently in the testing process. Wherein, the LED lamp can be replaced by a light emitting diode D1.
And the power supply module is connected with the anode of the LED lamp. In some embodiments, the power supply starting process is controlled by the switch key 250 by providing the switch key 250 on the upper surface of the housing. In addition, a USB interface 260 may be disposed on the upper surface of the housing, and the charging process of the power module and the data transmission process may be implemented through the USB interface 260.
After the test light source system of the above embodiment is placed on the microorganism reagent placing table in the microorganism luminescence tester and the light-transmitting hole is made to face the position where the photomultiplier tube obtains the light source, the operation principle of the above embodiment is as follows: the working state of the second adjustable resistor module is set according to the type of the microorganism tested by the microbial luminescence tester, namely, the multi-path selector is controlled by the controller to select the resistor with the corresponding resistance value to participate in working so as to control the large current flowing through the LED lamp and enable the light intensity emitted by the LED lamp to be within the light emitting intensity of the microorganism of the type, then, the first adjustable resistor module is controlled by the controller to finely adjust the resistance value so that the light intensity change of the LED lamp is slightly changed within the light emitting intensity of the microorganism of the type, and meanwhile, the data information displayed on the microbial luminescence tester is obtained so as to judge the performance of the microbial luminescence tester according to the data information and correct the performance of the microbial luminescence tester.
In some embodiments, in order to better realize coarse adjustment of the large current flowing through the LED lamp, a plurality of multiplexers are added, and a plurality of other resistors are controlled by the plurality of multiplexers, so that the control process of controlling the magnitude of the fine current by a simple circuit is realized through the parallel connection relationship between the resistors.
In some embodiments, since the light hole of the above embodiments is a fixed size, when the light intensity of the LED lamp cannot be changed by adjusting the resistor, the light intensity of the LED lamp is fixed and the light intensity obtained by the photomultiplier tube cannot be changed. Therefore, in order to further refine the range of the light intensity obtained by the photomultiplier tube, the system further comprises a slider 230, a driving module and a motor, wherein the input end of the driving module is connected with the controller, the output end of the driving module is connected with the motor, and the motor is used for controlling the sliding position of the slider.
This embodiment passes through the operating condition of controller control drive module, makes the position of drive module driving motor control slider, through the sheltering from of slider to the light trap, realizes the function that control photomultiplier acquires the size of light intensity.
In some embodiments, in order to better control the sliding process of the slider and to realize the light intensity control process, the slider may be shaped as a rectangle with a fan-shaped notch in the middle, through which the intensity of the transmitted light is controlled, and one side of the slider is shaped as a sawtooth, so that the motor can better control the moving process of the slider.
In some embodiments, in order to better control the sliding position of the sliding block 230, a sliding slot 231 is provided at the lower end of the housing, and the sliding block 230 slides in the sliding slot 231. Specifically, fig. 4 is a schematic view when the slider completely blocks the light hole; FIG. 5 is a schematic view of the slider partially blocking the light hole; fig. 6 is a schematic view of the slider not blocking the light hole at all.
In some embodiments, the system further includes a voltage stabilizing module, an input terminal of the voltage stabilizing module is connected to the power supply module, and an output terminal of the voltage stabilizing module is connected to the positive electrode of the LED lamp.
This implementation provides stable operating voltage for the LED lamp through constant voltage power supply.
Referring to fig. 7, an embodiment of the present invention further provides a detection method for a microbial luminescence tester, where the implementation may be applied to a service end, and detection is performed by using the above test light source system, and the service end may interact with the microbial luminescence tester and the test light source system respectively.
The implementation comprises the following steps:
s710, obtaining the type of the target microorganism; the type of the target microorganism is the type of the microorganism which can be tested by the microbial luminescence tester.
S720, setting the working state of a second adjustable resistance module according to the type of the target microorganism; specifically, the controller controls the resistance value of the second adjustable resistance module which participates in working according to the type of the target microorganism, so that the light intensity generated by the LED lamp is controlled within the light intensity range which can be emitted by the target microorganism according to the resistance value.
S730, adjusting the working state of the first adjustable resistance module; after the resistance value of the second adjustable resistance module participating in work is set, the resistance value participating in work is selected by adjusting the position of the upper sliding end of the first adjustable resistance module, so that the variation range of the LED lamp module is slightly changed within the light intensity range which can be emitted by target microorganisms.
S740, when the working state of the first adjustable resistance module is adjusted, reading the relative light intensity displayed by the microbial luminescence tester;
in some embodiments, after reading the relative light intensity displayed by the microbial luminescence tester, the method further comprises the following steps:
and adjusting the working state of the second adjustable resistance module.
In this embodiment, after the calibration is completed by the first adjustable resistance module under the current large resistance value of the second adjustable resistance module, the second adjustable resistance module is adjusted to select other large resistance values, so that the first adjustable resistance module can control the light intensity generated by the LED lamp under the conditions of other large resistance values to calibrate the performance of the microbial luminescence tester.
And S750, analyzing the working performance of the microbial luminescence tester according to the relative light intensity. The relative light intensity refers to a plurality of relative light intensity values read after the first adjustable resistance module and the second adjustable resistance module are adjusted repeatedly.
In some specific implementations, the test light source system implemented above is applied to an ATP fluorescence tester, and the specific implementation steps include:
the controller controls the resistance value selection of the second adjustable resistance module to be changed between 22Mohm and 70 Mohm. After each coarse adjustment gear is selected, the test light source system can automatically adjust the resistance value of the digital resistor according to the test time set by a program during calibration, namely, the resistance value of the first adjustable resistor module is adjusted, so that the LED lamp can be finely adjusted under the light intensity of the current coarse adjustment gear. And when the resistance value of the digital resistor is adjusted, the current of the LED lamp loop changes slightly, and further the relative light intensity value RLU read on the ATP fluorescence tester changes slightly, so that the resolution of the ATP fluorescence tester under the current light intensity can be verified.
After the digital resistor resistance adjustment under the current light intensity is set to run for a cycle according to a preset detection method, the gear of the second adjustable resistance module is adjusted to the next gear, and then the digital resistor continues to be adjusted in a cycle. And circulating the steps until the preset detection method is operated. At this time, the ATP fluorescence tester has measured a plurality of relative light intensity RLU values, and then the measured relative light intensity RLU values are analyzed, so that the resolution of the ATP fluorescence tester under a certain light intensity can be known.
When the resistance value of the digital resistor changes and the relative light intensity RLU value measured by the ATP fluorescence tester is basically unchanged, the preset detection method can be set to adjust the size of the light hole instead of adjusting the digital resistor, so that the light quantity irradiated by the LED lamp on the ATP fluorescence tester sensor is controlled, and then the test value is read and stored for analysis. In some embodiments, the detection method can also be set to allow the digital resistor and the light hole to be adjusted simultaneously.
In summary, in the embodiment of the system, the controller, the first adjustable resistance module, the second adjustable resistance module and the LED lamp are arranged, and the light emitting intensity of the LED lamp is controlled by controlling the resistance of the first adjustable resistance module and the second adjustable resistance module, so that the light emitting intensity of the LED lamp is close to the light emitting intensity of the microorganism, the performance detection process of the microbial luminescence tester can be completed without a test reagent, the reagent is saved, and an error caused by manually allocating the reagent is avoided.
While the preferred embodiments of the present invention have been illustrated and described, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A test light source system of a microbial light emitting tester, comprising:
the lower surface of the shell is provided with a light hole;
a controller disposed inside the housing;
the sliding end of the first adjustable resistance module is connected with the controller;
the control end of the second adjustable resistance module is connected with the controller, the first end of the second adjustable resistance module is connected with the first end of the first adjustable resistance module, and the second end of the second adjustable resistance module is grounded;
the cathode of the LED lamp is connected with the second end of the first adjustable resistor module, and the LED lamp is arranged in the shell and is opposite to the position of the light hole;
and the power supply module is connected with the anode of the LED lamp.
2. The system of claim 1, further comprising a slider, a driving module, and a motor, wherein an input end of the driving module is connected to the controller, and an output end of the driving module is connected to the motor, and the motor is used for controlling a sliding position of the slider.
3. The system as claimed in claim 2, wherein the housing has a sliding groove at a lower end thereof, and the slider slides in the sliding groove.
4. The testing light source system of a microbial luminous tester as claimed in claim 2, wherein the slider is shaped as a rectangle with a fan-shaped notch in the middle.
5. The system as claimed in claim 1, wherein the second adjustable resistor comprises a multiplexer and a plurality of resistors, a control terminal of the multiplexer is connected to the controller, an output terminal of the multiplexer is connected to the first terminal of the first adjustable resistor module, a plurality of switch terminals of the multiplexer are respectively connected to the first terminals of the plurality of resistors, and second terminals of the plurality of resistors are all grounded.
6. The system of claim 1, further comprising a voltage regulator module, wherein an input of the voltage regulator module is connected to the power supply module, and an output of the voltage regulator module is connected to the anode of the LED lamp.
7. A method for detecting a microbial luminous tester by the test light source system according to any one of claims 1 to 6, comprising the steps of:
obtaining the type of a target microorganism;
setting the working state of a second adjustable resistance module according to the type of the target microorganism;
adjusting the working state of the first adjustable resistance module;
when the working state of the first adjustable resistance module is adjusted, reading the relative light intensity displayed by the microbial luminescence tester;
and analyzing the working performance of the microbial luminescence tester according to the relative light intensity.
8. The method as claimed in claim 7, further comprising the following steps after reading the relative light intensity displayed by the bioluminescent test instrument:
and adjusting the working state of the second adjustable resistance module.
9. The method as claimed in claim 8, wherein the adjusting the operating status of the first adjustable resistance module comprises:
and controlling the position of the sliding end of the first adjustable resistance module through a controller.
10. The method as claimed in claim 7, wherein the setting of the operating state of the second adjustable resistance module is specifically as follows:
and selecting the working resistance of the second adjustable resistance module through a controller.
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| CN202010640456.XA CN111879739A (en) | 2020-07-06 | 2020-07-06 | Test light source system of microbial luminescence tester and detection method thereof |
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| CN202010640456.XA CN111879739A (en) | 2020-07-06 | 2020-07-06 | Test light source system of microbial luminescence tester and detection method thereof |
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