CN111875979A - Extraction method of plum blossom elm extract and application of plum blossom elm extract in tobacco sheets - Google Patents
Extraction method of plum blossom elm extract and application of plum blossom elm extract in tobacco sheets Download PDFInfo
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- CN111875979A CN111875979A CN202010914940.7A CN202010914940A CN111875979A CN 111875979 A CN111875979 A CN 111875979A CN 202010914940 A CN202010914940 A CN 202010914940A CN 111875979 A CN111875979 A CN 111875979A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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Abstract
The invention relates to the technical field of tobacco sheets, in particular to an extraction method of an extract of plum blossom leaves, which comprises the following steps: s1, cleaning fresh plum blossom leaves, drying in vacuum, spraying deionized water on the cooled plum blossom leaves after the vacuum drying is finished, and wetting the plum blossom leaves; s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder; s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding complex enzyme for enzymolysis after soaking, extracting an elm leaf plum extract A and an elm leaf plum extract B from the elm leaf plum by the method, wherein the elm leaf plum extract A can effectively change the color of the tobacco sheet, the elm leaf plum extract B can effectively improve the fragrance of the tobacco sheet and improve the taste of the tobacco sheet, and the elm leaf plum extract B also has the functions of clearing away the lung-heat and moistening the throat, so that the harm of the tobacco sheet can be reduced to a certain extent.
Description
Technical Field
The invention relates to the technical field of tobacco sheets, in particular to an extraction method of an extract of plum blossom leaves and application of the extract in tobacco sheets.
Background
The tobacco sheet is a regenerated tobacco leaf which is formed by recombining and processing tobacco waste materials such as tobacco powder, tobacco stems, inferior tobacco leaves and the like in the cigarette manufacturing process including a threshing process in a tobacco factory as a raw material. The tobacco sheet finished product dipped or sprayed by the concentrated solution can be blended with natural tobacco leaves to be processed into cigarettes, so that the tobacco leaf raw materials can be utilized to the maximum extent, the cigarette cost is effectively reduced, the physical and chemical properties of the cigarettes can be improved to a certain extent, and the quality of the cigarettes is improved. The current preparation methods of the tobacco sheets mainly comprise a paper making method, a thick pulp method and a rolling method. The typical production process of the tobacco sheet by the paper-making method comprises the steps of soaking raw materials in water, extracting and separating soluble matters from insoluble matters, pulping the insoluble matters tobacco fibers, making into a sheet base by papermaking, spraying the extract of the soluble matters onto the sheet base after concentration, and drying/cutting to obtain the tobacco sheet. Compared with other two methods, the tobacco sheet prepared by the paper making method has the characteristics of adjustable tobacco components, good combustion performance, low tar release amount, small product density, good filling property and the like, thereby being popular with cigarette manufacturers and consumers.
However, in the preparation of the tobacco sheet by the paper making method, the concentrated extract liquor is directly dipped or sprayed on the sheet base of the sheet, and the product has the defects of heavy miscellaneous gas and wood gas, obvious dry sensation at the throat, obvious pungent sensation at the tongue and the like.
Therefore, we propose an extraction method of the extract of plum blossom elm and its application in tobacco sheets to solve the above problems.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides an extracting method of an extract of plum blossom leaves and application of the extract in tobacco sheets.
A method for extracting an extract of plum blossom leaf of ulmus pumila comprises the following steps:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 80-90 ℃ for 4-6 h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying is finished;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 3-4, soaking for 2-4 h, adding a complex enzyme for enzymolysis after soaking, wherein the enzymolysis time is 30-60 min, filtering after enzymolysis, and collecting filter residue and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 50-60 ℃, extracting by using an extracting agent which is a mixture of walnut oil and ethyl acetate with the same volume as the filtrate, wherein the ratio of the walnut oil to the ethyl acetate is 1:40-50, combining the extracting solutions after 2 times of extraction, finally concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, carrying out vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, carrying out reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, carrying out reduced pressure recovery to obtain an extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 50% -60% ethanol until the eluent is nearly colorless to obtain an ethanol eluent, and recycling the ethanol from the ethanol eluent to obtain an extract B of the plum blossom elm leaf.
Preferably, in S1, the mass ratio of the deionized water to the plum blossom leaves is (0.5-1): 1.
preferably, in the S2, the mesh number of the screen in the screening process is 80-100 meshes.
Preferably, in the step S3, in the soaking process, ultrasonic treatment is performed for 30min to 45 min.
Preferably, in the S3, the complex enzyme is pectinase, a complex plant hydrolase, and acid protease in a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is (0.001-0.003): 1.
preferably, in S4, the extracted liquid is filtered, and the residue is discarded.
Preferably, the plum blossom ulmus pumila extract A is added into the tobacco sheets, and the addition amount of the plum blossom ulmus pumila extract A is 0.01-0.03% of the weight of the tobacco sheets.
Preferably, the plum blossom ulmus pumila extract B is added into the tobacco sheets, and the addition amount of the plum blossom ulmus pumila extract B is 0.2-2.5% of the weight of the tobacco sheets.
Preferably, the plum blossom ulmus pumila extract A and the plum blossom ulmus pumila extract B are simultaneously added into the tobacco sheets, the addition amount of the plum blossom ulmus pumila extract A is 0.01-0.03% of the weight of the tobacco sheets, and the addition amount of the plum blossom ulmus pumila extract B is 0.2-2.5% of the weight of the tobacco sheets.
The invention has the beneficial effects that:
1. the plum blossom extract A and the plum blossom extract B are extracted from plum blossom elm by the method, the plum blossom extract A can effectively change the color of the tobacco sheet, the plum blossom extract B can effectively improve the fragrance of the tobacco sheet and improve the taste of the tobacco sheet, and the plum blossom extract B also has the functions of clearing away the lung-heat and moistening the throat, so that the harm of the tobacco sheet can be reduced to a certain extent.
2. According to the method, the plum blossom leaves are dried, the plum blossom leaves after the drying treatment are wetted, the plum blossom leaves can be reamed by the freeze-drying treatment, subsequent extraction of effective components is facilitated, the tissue structure of the plum blossom leaves can be further damaged by the enzymolysis process, the subsequent extraction of the effective components is further facilitated, and the protein can be decomposed by the enzymolysis process, so that the content of the protein in the plum blossom leaf extract B is low, and the tobacco sheets cannot be adversely affected.
3. In the method, when the plum blossom extract A is extracted, the plum blossom extract A (plum blossom pigment) is extracted through a mixture of walnut oil and ethyl acetate, the walnut oil has high solubility to the plum blossom pigment, the extraction efficiency of the ethyl acetate can be effectively improved, the ethyl acetate is volatile during subsequent treatment, the residual walnut oil can improve the taste of the tobacco sheet, and the tobacco sheet has a certain function of increasing fragrance.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
In example 1, a method for extracting an extract of plum blossom leaf elm comprises the following steps:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 80 ℃ for 4h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 3, soaking for 2 hours, adding a complex enzyme for enzymolysis after soaking, wherein the enzymolysis time is 30min, filtering after enzymolysis, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 50 ℃, extracting for 2 times by using an extracting agent which is a mixture of walnut oil and ethyl acetate and has the same volume with the filtrate, wherein the extracting agent is the mixture of the walnut oil and the ethyl acetate, the ratio of the walnut oil to the ethyl acetate is 1:40, combining the extracting solutions, concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 50% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 0.5: 1.
further, in S2, the mesh number of the screen in the screening process is 80 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 30 min.
Further, in the S3, the compound enzyme is pectinase, compound plant hydrolase and acid protease according to a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is 0.001: 1.
further, in S4, the extracted liquid is filtered, and the residue is discarded.
In example 2, a method for extracting an extract of plum blossom leaf elm comprises the following steps:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 90 ℃ for 6 hours, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 4, soaking for 4 hours, adding a complex enzyme for enzymolysis after soaking is finished, wherein the enzymolysis time is 60min, filtering after enzymolysis is finished, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 60 ℃, extracting by using an extracting agent which is a mixture of walnut oil and ethyl acetate with the volume equal to that of the filtrate, wherein the ratio of the walnut oil to the ethyl acetate is 1:40-50, mixing the extracting solutions after 2 times of extraction, finally concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, passing the ethanol extracting solution through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 60% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 1: 1.
further, in S2, the mesh number of the screen in the screening process is 100 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 45 min.
Further, in the S3, the compound enzyme is pectinase, compound plant hydrolase and acid protease according to a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is 0.003: 1.
further, in S4, the extracted liquid is filtered, and the residue is discarded.
In example 3, a method for extracting an extract of plum blossom leaf elm comprises the following steps:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 85 ℃ for 5h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 4, soaking for 3 hours, adding a complex enzyme for enzymolysis after soaking is finished, wherein the enzymolysis time is 45min, filtering after enzymolysis is finished, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 55 ℃, extracting for 2 times by using an extracting agent which is a mixture of walnut oil and ethyl acetate and has the same volume with the filtrate, wherein the extracting agent is the mixture of the walnut oil and the ethyl acetate, the ratio of the walnut oil to the ethyl acetate is 1:45, combining the extracting solutions, concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 55% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 0.7: 1.
further, in S2, the mesh number of the screen in the screening process is 80 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 40 min.
Further, in the S3, the compound enzyme is pectinase, compound plant hydrolase and acid protease according to a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is 0.002: 1.
further, in S4, the extracted liquid is filtered, and the residue is discarded.
Comparative example 1, a method for extracting an extract of plum blossom leaf elm, comprising the steps of:
s1, cleaning fresh plum blossom leaves, and then carrying out vacuum drying at the temperature of 85 ℃ for 5 h;
s2, after vacuum drying, crushing and sieving the plum blossom leaves to obtain plum blossom leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 4, soaking for 3 hours, adding a complex enzyme for enzymolysis after soaking is finished, wherein the enzymolysis time is 45min, filtering after enzymolysis is finished, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 55 ℃, extracting for 2 times by using an extracting agent which is a mixture of walnut oil and ethyl acetate and has the same volume with the filtrate, wherein the extracting agent is the mixture of the walnut oil and the ethyl acetate, the ratio of the walnut oil to the ethyl acetate is 1:45, combining the extracting solutions, concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 55% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 0.7: 1.
further, in S2, the mesh number of the screen in the screening process is 80 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 40 min.
Further, in the S3, the compound enzyme is pectinase, compound plant hydrolase and acid protease according to a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is 0.002: 1.
further, in S4, the extracted liquid is filtered, and the residue is discarded.
Comparative example 2, a method for extracting an extract of plum blossom, elm leaf, comprising the steps of:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 85 ℃ for 5h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 4, soaking for 3 hours, filtering after the soaking is finished, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 55 ℃, extracting for 2 times by using an extracting agent which is a mixture of walnut oil and ethyl acetate and has the same volume with the filtrate, wherein the extracting agent is the mixture of the walnut oil and the ethyl acetate, the ratio of the walnut oil to the ethyl acetate is 1:45, combining the extracting solutions, concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 55% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 0.7: 1.
further, in S2, the mesh number of the screen in the screening process is 80 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 40 min.
Further, in S4, the extracted liquid is filtered, and the residue is discarded.
Comparative example 3, a method for extracting an extract of plum blossom leaf elm, comprising the steps of:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 85 ℃ for 5h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 4, soaking for 3 hours, adding a complex enzyme for enzymolysis after soaking is finished, wherein the enzymolysis time is 45min, filtering after enzymolysis is finished, and collecting filter residues and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the frozen and stored filtrate to 55 ℃, extracting by using an extracting agent which is the same as the filtrate in volume, wherein the extracting agent is ethyl acetate, mixing the extracting solutions after 2 times of extraction, finally concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum extract A, namely elm leaf plum pigment;
s5, washing the filter residue with deionized water, performing vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, performing reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, recovering under reduced pressure to obtain the extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 55% ethanol until the eluate is nearly colorless to obtain an ethanol eluate, and recovering ethanol from the ethanol eluate to obtain an extract B of plum blossom elm leaf.
Further, in the step S1, the mass ratio of the deionized water to the plum blossom leaves is 0.7: 1.
further, in S2, the mesh number of the screen in the screening process is 80 meshes.
Further, in the step S3, in the soaking process, ultrasonic treatment is performed for 40 min.
Further, in the S3, the compound enzyme is pectinase, compound plant hydrolase and acid protease according to a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is 0.002: 1.
further, in S4, the extracted liquid is filtered, and the residue is discarded.
In examples 1-3, an application of a plum blossom ulmoides extract in a tobacco sheet is that plum blossom ulmoides extract A is added into the tobacco sheet, and the addition amount of the plum blossom ulmoides extract A is 0.01-0.03% of the weight of the tobacco sheet.
An application of a plum blossom ulmus pumila extract in a tobacco sheet is characterized in that the plum blossom ulmus pumila extract B is added into the tobacco sheet, and the addition amount of the plum blossom ulmus pumila extract B is 0.2% -2.5% of the weight of the tobacco sheet.
An application of a plum blossom ulmus pumila extract in a tobacco sheet is characterized in that the plum blossom ulmus pumila extract A and the plum blossom ulmus pumila extract B are added into the tobacco sheet at the same time, the addition amount of the plum blossom ulmus pumila extract A is 0.01-0.03% of the weight of the tobacco sheet, and the addition amount of the plum blossom ulmus pumila extract B is 0.2-2.5% of the weight of the tobacco sheet.
In examples 1 to 3 and comparative examples 1 to 3, the obtained plum blossom extract a and plum blossom extract B were added to tobacco sheets, and the amount of each added group was the same, wherein the amount of the plum blossom extract a added was 0.02% by weight of the tobacco sheets, and the amount of the plum blossom extract B added was 1.2% by weight of the tobacco sheets, and sensory quality evaluations of color and texture were performed with the tobacco sheets made without the plum blossom extract of the present invention, and the results are shown in table 1 below.
As can be seen by comparing examples 1-3 with untreated tobacco sheets, the color and taste of the tobacco sheets added with the plum blossom extract A and the plum blossom extract B are obviously improved, the quality of the tobacco sheets can be effectively improved, in the comparative example 1, freeze-drying treatment is not carried out in the extraction process, the quality of the extracted plum blossom extract A and plum blossom extract B is slightly reduced, the color and taste of the tobacco sheets are lower than those of the examples 1-3, in the comparative example 2, no complex enzyme is added in the extraction process for enzymolysis, part of protein is mixed into the plum blossom extract B, the taste of the tobacco sheets is lower than those of the examples 1-3, in the comparative example 3, the extractant does not contain walnut oil in the extraction process, the extraction effect of the plum blossom extract A is slightly poor, the color of the tobacco sheets is lower than those of the examples 1-3, and some of the foreign flavor of the tobacco sheet cannot be improved by walnut oil.
Table 1: sensory quality evaluation results of color and taste of tobacco sheet
As can be seen by comparing examples 1-3 with untreated tobacco sheets, the color and taste of the tobacco sheets added with the plum blossom extract A and the plum blossom extract B are obviously improved, the quality of the tobacco sheets can be effectively improved, in the comparative example 1, freeze-drying treatment is not carried out in the extraction process, the quality of the extracted plum blossom extract A and plum blossom extract B is slightly reduced, the color and taste of the tobacco sheets are lower than those of the examples 1-3, in the comparative example 2, no complex enzyme is added in the extraction process for enzymolysis, part of protein is mixed into the plum blossom extract B, the taste of the tobacco sheets is lower than those of the examples 1-3, in the comparative example 3, the extractant does not contain walnut oil in the extraction process, the extraction effect of the plum blossom extract A is slightly poor, the color of the tobacco sheets is lower than those of the examples 1-3, and some of the foreign flavor of the tobacco sheet cannot be improved by walnut oil.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (9)
1. The extraction method of the plum blossom ulmoides extract is characterized by comprising the following steps of:
s1, cleaning fresh plum blossom leaves, drying in vacuum at 80-90 ℃ for 4-6 h, and spraying deionized water to the cooled plum blossom leaves to wet the plum blossom leaves after vacuum drying is finished;
s2, freeze-drying the wetted plum blossom leaves, crushing and sieving after the freeze-drying treatment is finished to obtain plum blossom elm leaf powder;
s3, mixing the plum blossom ulmoides powder and deionized water according to the weight ratio of 1: 5, adding a proper amount of hydrochloric acid, adjusting the pH value to 3-4, soaking for 2-4 h, adding a complex enzyme for enzymolysis after soaking, wherein the enzymolysis time is 30-60 min, filtering after enzymolysis, and collecting filter residue and filtrate;
s4, freezing and storing the filtrate at-18 ℃ for 4h, heating the filtrate after freezing and storing to 50-60 ℃, extracting by using an extracting agent which is a mixture of walnut oil and ethyl acetate with the same volume as the filtrate, wherein the ratio of the walnut oil to the ethyl acetate is 1:40-50, combining the extracting solutions after 2 times of extraction, finally concentrating the extracting solution to an extract state, and performing vacuum drying to obtain an elm leaf plum blossom extract A, namely elm leaf plum blossom pigment;
s5, washing the filter residue with deionized water, carrying out vacuum drying treatment, adding 8 times of 75% ethanol into the filter residue after the drying treatment is finished, carrying out reflux extraction for 3 times, each time for 2 hours, filtering, combining the extracting solutions, carrying out reduced pressure recovery to obtain an extracting solution, enabling the ethanol extracting solution to pass through an AB-8 macroporous resin column, washing with deionized water until the effluent liquid is transparent, eluting with 50% -60% ethanol until the eluent is nearly colorless to obtain an ethanol eluent, and recycling the ethanol from the ethanol eluent to obtain an extract B of the plum blossom elm leaf.
2. The extraction method of plum blossom extract according to claim 1, wherein in S1, the mass ratio of deionized water to plum blossom is (0.5-1): 1.
3. the method for extracting an extract of plum blossom leaf of elm as claimed in claim 1, wherein the mesh number of the screen is 80-100 mesh in the sieving process in S2.
4. The extraction method of the plum blossom extract as claimed in claim 1, wherein in the step of S3, ultrasonic treatment is performed during the soaking process, and the ultrasonic treatment time is 30min to 45 min.
5. The extraction method of an extract of plum blossom of elm according to claim 1, wherein in S3, the complex enzyme is pectinase, complex plant hydrolase and acid protease in a mass ratio of 1: 2: 0.5, the mass ratio of the complex enzyme to the elm leaf plum powder is (0.001-0.003): 1.
6. the method for extracting an extract of plum blossom leaves of elm according to claim 1, wherein in S4, the extract after extraction is filtered, and the residue is discarded.
7. The use of a plum blossom extract in a tobacco sheet as claimed in any one of claims 1 to 6, wherein the plum blossom extract A is added to the tobacco sheet in an amount of 0.01% to 0.03% by weight of the tobacco sheet.
8. The use of a Prunus ulmaria extract in tobacco sheets as claimed in any one of claims 1-6, wherein the Prunus ulmaria extract B is added into the tobacco sheets in an amount of 0.2-2.5% by weight of the tobacco sheets.
9. The use of a Prunus ulmaria extract in tobacco sheets as claimed in any one of claims 1-6, wherein the Prunus ulmaria extract A and the Prunus ulmaria extract B are added into the tobacco sheets at the same time, the amount of the Prunus ulmaria extract A is 0.01-0.03% of the weight of the tobacco sheets, and the amount of the Prunus ulmaria extract B is 0.2-2.5% of the weight of the tobacco sheets.
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| US20100119651A1 (en) * | 2008-11-12 | 2010-05-13 | Fan Shufeng | Bio-Enzyme-Assisted Extraction Method for Inulin |
| CN102423124A (en) * | 2011-11-01 | 2012-04-25 | 吉林烟草工业有限责任公司 | Tobacco slice and preparation method thereof |
| CN103478885A (en) * | 2013-09-27 | 2014-01-01 | 福建中烟工业有限责任公司 | Tobacco sheet containing tea flower extractive and preparation method thereof |
| CN107668715A (en) * | 2017-10-31 | 2018-02-09 | 徐州绿之野生物食品有限公司 | A kind of food additives with regulation blood glucose effect and application |
| CN108095186A (en) * | 2017-09-26 | 2018-06-01 | 河北瑞龙生物科技有限公司 | One grows tobacco with essence, preparation method and tobacco product |
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| US20100119651A1 (en) * | 2008-11-12 | 2010-05-13 | Fan Shufeng | Bio-Enzyme-Assisted Extraction Method for Inulin |
| CN102423124A (en) * | 2011-11-01 | 2012-04-25 | 吉林烟草工业有限责任公司 | Tobacco slice and preparation method thereof |
| CN103478885A (en) * | 2013-09-27 | 2014-01-01 | 福建中烟工业有限责任公司 | Tobacco sheet containing tea flower extractive and preparation method thereof |
| CN108095186A (en) * | 2017-09-26 | 2018-06-01 | 河北瑞龙生物科技有限公司 | One grows tobacco with essence, preparation method and tobacco product |
| CN107668715A (en) * | 2017-10-31 | 2018-02-09 | 徐州绿之野生物食品有限公司 | A kind of food additives with regulation blood glucose effect and application |
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