CN111869664B - Composition for inducing growth of apocarya seedlings and preparation and application thereof - Google Patents
Composition for inducing growth of apocarya seedlings and preparation and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 239000000203 mixture Substances 0.000 title claims abstract description 28
- 230000001939 inductive effect Effects 0.000 title claims abstract description 24
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical group C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims abstract description 57
- 239000005985 Paclobutrazol Substances 0.000 claims abstract description 57
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims abstract description 55
- 235000009025 Carya illinoensis Nutrition 0.000 claims abstract description 40
- 241001453450 Carya illinoinensis Species 0.000 claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 35
- 239000000375 suspending agent Substances 0.000 claims description 3
- 239000012752 auxiliary agent Substances 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 4
- 239000000725 suspension Substances 0.000 claims 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 15
- 230000001737 promoting effect Effects 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000002786 root growth Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 23
- 239000012153 distilled water Substances 0.000 description 21
- 238000002156 mixing Methods 0.000 description 20
- 238000005507 spraying Methods 0.000 description 18
- 238000011282 treatment Methods 0.000 description 15
- 241000196324 Embryophyta Species 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000005556 hormone Substances 0.000 description 6
- 229940088597 hormone Drugs 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
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- 238000000034 method Methods 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 239000004552 water soluble powder Substances 0.000 description 5
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 238000010306 acid treatment Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 2
- 230000002015 leaf growth Effects 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 229930191978 Gibberellin Natural products 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011369 optimal treatment Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a composition for inducing growth of apocarya seedlings and a preparation and application thereof, belonging to the technical field of agriculture. The composition for inducing the growth of the apocarya seedlings consists of an agent A and an agent B; wherein the agent A is a naphthylacetic acid solution, the agent B is a paclobutrazol solution, and the concentration ratio of the agent A to the agent B is 0.5: 1. In addition, when the agent A is specifically applied, the agent A is sprayed on the roots of the carya illinoensis seedlings, and the agent B is sprayed on the true leaves of the carya illinoensis seedlings. Experiments show that the composition has good effect of promoting the ground diameter and the chlorophyll content and also has good effect of promoting the root growth, can be applied to actual production, and is beneficial to promoting the cultivation of good-quality grafted strong seedlings in the future.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a composition for inducing growth of apocarya seedlings, and a preparation and application thereof.
Background
The area of the existing carya illinoensis in Anhui province exceeds 40 ten thousand mu, the area will continue to increase continuously in the future, and a large amount of seeds and seedlings are urgently needed in the market. And the carya illinoensis afforestation needs to use good-variety grafted seedlings, so that the quality of the good-variety grafted seedlings is improved, and the condition that the stocks are healthy and strong is very important to ensure. Generally, the seed cultivation seedling is used as a stock for grafting, and most plants above the ground after grafting need to be cut off to cultivate scion by nutrition absorbed by the root system. The carya illinoensis seedlings need support of multiple elements in the growth process, and domestic and foreign researches find that the plant growth regulator also has a regulating effect on the growth of plants, for example, gibberellin exists in a place where plant growth embryos are abundant, and can promote the growth of stems, break dormancy, promote germination and fruit setting, induce flowering of plants and the like. The method is characterized in that Gibberellin (GA), indolebutyric acid (IBA) and indoleacetic acid (IAA) are applied to seedlings of carya illinoensis domestically, and a plurality of hormones are applied to the seedlings of carya illinoensis, so that the germination rates of the carya illinoensis seedlings and seeds treated by different hormones are obviously different. Particularly, the carya illinoensis seedling cultured as a stock has no targeted strong seedling hormone spraying technology.
Disclosure of Invention
1. Problems to be solved
Aiming at the problems in the prior art, the invention provides the composition for inducing the growth of the apocarya seedlings, the preparation and the application thereof, wherein the composition is reasonable in components and low in cost.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A composition for inducing the growth of carya illinoensis seedlings comprises an agent A and an agent B;
wherein the agent A is a naphthylacetic acid solution, the agent B is a paclobutrazol solution, and the concentration ratio of the agent A to the agent B is 0.5: 1.
In the composition for inducing growth of the carya illinoensis seedlings, the concentration range of the naphthylacetic acid solution is 150mg/L-200mg/L, and the concentration range of the paclobutrazol solution is 200mg/L-400 mg/L.
In the composition for inducing growth of the carya illinoensis seedlings, the concentration range of the naphthylacetic acid solution is 100mg/L, and the concentration range of the paclobutrazol solution is 200 mg/L.
In the composition for inducing growth of the carya illinoensis seedlings, the concentration range of the naphthylacetic acid solution is 200mg/L, and the concentration range of the paclobutrazol solution is 400 mg/L.
A preparation for inducing growth of carya illinoensis seedlings is prepared from any one of the compositions and an auxiliary agent, and the preparation is any one of a suspending agent, missible oil and an aqueous emulsion.
In the preparation for inducing the growth of the carya illinoensis seedlings, the preparation is a suspending agent.
Use of a composition as described above for inducing the growth of carya illinoensis seedlings.
Application of the preparation in inducing the growth of apocarya seedlings.
In the application of the preparation in inducing the growth of the carya illinoensis seedlings, the agent A is sprayed on the roots of the carya illinoensis seedlings, and the agent B is sprayed on the true leaves of the carya illinoensis seedlings.
In the application of the preparation in inducing the growth of the carya illinoensis seedlings, the carya illinoensis seedlings are seedlings with true leaves.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
after the analysis of all factors, the concentration ratio of the naphthylacetic acid solution in the agent A to the paclobutrazol solution in the agent B is 0.5: 1, particularly the following two groups: the concentration of the naphthylacetic acid solution is 100mg/L, the concentration of the paclobutrazol solution is 200mg/L, the concentration of the naphthylacetic acid solution is 200mg/L and the concentration of the paclobutrazol solution is 400mg/L, the promotion effect on the ground diameter and the chlorophyll content is good, meanwhile, the effect of promoting the growth of a root system is good, the method can be applied to actual production, and the method is favorable for promoting the cultivation of good-quality grafted strong seedlings in the future.
Detailed Description
The invention is further described with reference to specific examples.
Example 1
Test materials:
the test carya illinoensis seedlings are seedlings grown in the same year (2019) in a 'one-two-seedling' seedling pool of Shunyuan agriculture limited company in Changfeng county, Hefei, Anhui province. The plant growth regulator is 20% naphthylacetic acid powder and 15% paclobutrazol powder produced by Sichuan Guanghuang agricultural and chemical Co., Ltd (GG).
Preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare solution A (agent A) with the concentration of 100mg/L, and mixing paclobutrazol and distilled water to prepare solution B (agent B) with the concentration of 200mg/L to obtain the final product.
Spraying treatment:
before treatment, 10 seedlings (i.e. seedlings with true leaves) are randomly selected from a nursery pond, the main root length (cm), the seedling height (cm) and the ground diameter (mm) of the seedlings are measured and recorded, and three true leaves of the seedlings are randomly selected to measure the leaf area (cm) of the leaves2) Leaf width (cm), leaf length (cm) and relative content of chlorophyll. And spraying the solution A on the roots of the carya illinoensis seedlings until the root soil is completely soaked, and spraying the solution B on the true leaves of the carya illinoensis seedlings until the leaves drip. The spraying treatment is carried out once every 15d, after 30d, the main root length (cm), the seedling height (cm) and the ground diameter (mm) of the seedling are measured and recorded again, and three true leaves of the seedling are randomly selected to determine the leaf area (cm) of the leaf2) Leaf width (cm), leaf length (cm) and relative content of chlorophyll,
it should be noted that the amount of paclobutrazol should be carefully controlled during spraying to prevent the paclobutrazol in the solution B from largely permeating into the soil.
Data processing: related data analysis is carried out by adopting Excel 2003 and SPSS 19.0, and the significance and the relevance of the difference among various groups of data are obtained.
Example 2
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 100mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 300mg/L to obtain the paclobutrazol-containing aqueous solution A.
Example 3
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 100mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 400mg/L to obtain the paclobutrazol-containing aqueous solution A.
Example 4
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 150mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 200mg/L to obtain the paclobutrazol-containing water-soluble powder.
Example 5
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 150mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 300mg/L to obtain the paclobutrazol-containing water-soluble powder.
Example 6
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 150mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 400mg/L to obtain the paclobutrazol-containing aqueous solution A.
Example 7
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 200mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 200mg/L to obtain the paclobutrazol-containing water-soluble powder.
Example 8
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 200mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 300mg/L to obtain the paclobutrazol-containing water-soluble powder.
Example 9
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid and distilled water to prepare a solution A with the concentration of 200mg/L, and mixing paclobutrazol and distilled water to prepare a solution B with the concentration of 400mg/L to obtain the paclobutrazol-containing water-soluble powder.
Example 10
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid with distilled water to obtain solution A with concentration of 100 mg/L.
Example 11
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
mixing naphthylacetic acid with distilled water to obtain solution B with concentration of 200 mg/L.
Example 12
The present embodiment is basically the same as embodiment 1, except that:
preparation of a preparation:
preparing distilled water into solution A and solution B respectively.
Example 13
The composition of the naphthylacetic acid and the paclobutrazol under different concentration ratios has the influence on various physiological indexes of the apocarya seedlings
And (4) analyzing results:
the data from examples 1-12 were analyzed for collation and statistics as follows,
TABLE 1 growth index of Carya illinoensis seedlings at different concentration ratios of naphthylacetic acid to paclobutrazol in the composition
TABLE 2 variation and growth rate of the length of the main root of Carya illinoensis seedling at different concentration ratios of naphthylacetic acid and paclobutrazol in the composition
As can be seen from tables 1 and 2, the application of the naphthylacetic acid and the paclobutrazol in different concentration ratios has great influence on various physiological indexes of the carya illinoensis, and the difference between the different concentration ratios is large, particularly in the aspects of main root length, seedling height, ground diameter, chlorophyll, leaf area, leaf width, leaf length and the like of one-time seedlings (seedlings). It is to be noted that the setup of example 10 and example 11 was intended to provide a control of single spray application of naphthylacetic acid or paclobutrazol, and the setup of example 12 was intended to provide a blank control.
Length of main root
After 30d of spraying treatment, the main root length of the carya illinoensis seedlings of example 1(40.37cm), example 3(37.63cm), example 5(34.87cm), example 6(35.54cm), example 8(36.97cm) and example 9(39.30cm) is greater than that of example 12(34.87cm), and the main root growth of the other three examples except the six examples is inhibited to different degrees, but in each group in which the main root length is promoted, only the values of example 1, example 8 and example 9 are significantly higher than that of the blank group (P < 0.05), the highest net growth rate reaches 56.40% (shown in Table 2), while the other three examples do not reach a significant level (P < 0.05), and the five groups with the main root length lower than that of example 12 do not reach the significant level (P < 0.05). Experimental results show that 100mg/L and 200mg/L of naphthylacetic acid can promote the growth of main roots, and particularly the effect of promoting the growth of root systems after the 100mg/L of naphthylacetic acid is matched with 200mg/L of paclobutrazol is the best, which is not disclosed in the prior art. In addition, 200mg/L naphthylacetic acid can also play a good role in root growth after being matched with 400mg/L paclobutrazol. The effect of spraying the naphthylacetic acid alone is better, but is not as good as that of the optimal treatment group, and the effect of spraying the paclobutrazol alone has even inhibition effect on the growth of the root length, which is not as good as that of the root length of the blank control group.
Height of seedling
After 30 days of spraying treatment, the average seedling height of 9 treatment groups was lower than that of the blank group. Among them, example 4(23.27cm), example 5(22.17cm), example 6(21.73cm) and example 7(20.07cm) were significant at a level of 0.05, and the high growth rate of the seedlings was the lowest in example 7.
The paclobutrazol solution sprayed on the leaf surfaces plays a main inhibiting role, and experimental results show that paclobutrazol with the concentration within the range of 200mg/L-400mg/L can effectively inhibit the growth of seedling height, wherein the paclobutrazol with the concentration within the range of 200mg/L-300mg/L has a better inhibiting effect, the inhibiting effect on the growth of the seedling height is most obvious when the paclobutrazol with the concentration of 200mg/L and naphthylacetic acid with the concentration of 200mg/L is matched, and the average seedling height is reduced by 11.86 cm. From the analysis of naphthylacetic acid, the high mean value of each group matched with 100mg/L naphthylacetic acid is the largest, and 100mg/L naphthylacetic acid is also the optimal concentration for promoting the growth of root length by combining the related data of main root length, so that the higher seedling height at the concentration is probably influenced by the correlation and the cooperativity of the growth of the overground part and the underground part of the plant.
Ground diameter
After 30d of spraying treatment, the land diameters of example 1(5.35mm), example 4(5.60mm), example 7(5.50mm), example 8(7.43mm) and example 9(7.94mm) were higher than those of the blank group in 9 treatment groups, wherein the land diameters of example 8 and example 9 were significantly different (P < 0.05), and the land diameters of the rest groups were lower than those of the blank group and were not significantly different.
The ground diameter is one of the criteria for judging strong seedlings, the ground diameter and the height of the seedlings belong to the physiological indexes of the overground part, the ground diameter and the height of the seedlings have good correlation, but the ground diameter and the height of the seedlings are not obvious in the experiment, and the application of hormones plays a role in interference to a certain extent. Experimental results show that the ground diameter growth amount is higher after the treatment of matching 200mg/L naphthylacetic acid with three concentrations of paclobutrazol (200mg/L-400mg/L), wherein the promotion effect is the best after the matching application of 200mg/L naphthylacetic acid and 400mg/L paclobutrazol. The diameter of each group after the matching treatment of 200mg/L paclobutrazol and naphthylacetic acid with three concentrations (100mg/L-200mg/L) is larger than that of the blank group.
Chlorophyll
After 30d of spraying treatment, except for example 1 and example 6, the relative content of chlorophyll in the other 7 groups is greater than that in the blank group without hormone application, wherein the content of the ninth group is the highest and is significantly higher than that in the blank group (P < 0.05), and the difference of the remaining 8 groups is not significant.
The relative content of chlorophyll of the plant is positively correlated with the photosynthetic rate, the photosynthesis is the main way for producing organic matters by the plant, along with the increase of the photosynthetic rate, the accumulation of the organic matters of the plant is correspondingly improved, and the growth is accelerated. In the experiment, the chlorophyll content of the leaves is increased after most concentration groups are treated, wherein the promoting effect of matching and applying 200mg/L of naphthylacetic acid and 400mg/L of paclobutrazol is the best. However, the photosynthetic yield of a plant is not only related to the chlorophyll content but also affected by factors such as leaf area, and cannot be used alone as a standard for judging the growth rate of the plant. The research shows that the chlorophyll content can be promoted by the compound application of 100-200mg/L naphthylacetic acid and 200-400mg/L paclobutrazol.
Leaf area, leaf width, leaf length
After 30 days of spraying treatment, the average value of leaf area and the average value of leaf length of 9 treatment groups are lower than that of a blank group. Example 4, example 5, example 6, example 7, example 9 in leaf area reached significance at the 0.05 level, with example 6 leaf area being the smallest. Example 5, example 6, example 7, example 9 of the leaf lengths reached significance at the 0.05 level, with example 9 having the shortest leaf length. The leaf width means were lower in all 8 groups than in the blank except that example 2 was slightly higher than in the blank, with example 5, example 6, example 7, example 9 reaching significance at a level of 0.05, with example 5 having the shortest leaf width.
The leaf area, leaf width, leaf length and seedling height belong to the phenotypic traits of the overground part of the seedling, and have certain relevance in growth, and the data shows that the growth of the leaves of each treatment group is the same as the seedling height and is inhibited to different degrees, which shows that the overground part growth is effectively inhibited by spraying paclobutrazol on the leaf surfaces. According to the concentration analysis of the naphthylacetic acid, the leaf growth inhibition effect of each group of seedlings is obvious after 150mg/L-200mg/L naphthylacetic acid treatment, and the inhibition effect of three groups after 100mg/L naphthylacetic acid treatment is poor, which is probably caused by the low promotion and high inhibition effect of the naphthylacetic acid. When the concentration of the naphthylacetic acid is the same, the higher concentration of paclobutrazol (300mg/L-400mg/L) has stronger inhibiting effect on the growth of the leaves. However, the leaf growth inhibitory effect is a result of the synergistic action of two hormones, and is also influenced by other factors such as organ growth, and several groups considered to have significant inhibitory effects are the result of the combined action of high-concentration naphthylacetic acid and high-concentration paclobutrazol from the viewpoint of multifactorial analysis.
In the experiment, paclobutrazol plays a good role in inhibiting stems and leaves on the upper part of the ground, the seedling height, the leaf area, the leaf width and the leaf length of each group are reduced to different degrees, but the chlorophyll content of each group is not reduced, even the trend of rising appears, and the change is possibly related to the promoted root system growth.
The Naphthalene Acetic Acid (NAA) is used as a plant growth promoter and mainly acts on roots in the experiment to promote the elongation growth of main roots, and from the experimental data, the NAA with different concentrations has different promotion effects on the roots, the promotion effect is better in the concentration range of 100-200mg/L, and the inhibition effect occurs at higher concentration. The land diameter is different from the reaction of different concentration groups, and experiments show that the land diameter of each group sprayed with 200mg/L NAA is higher than that of the blank group, wherein the thickening effect is best when the land diameter is matched with 300-400mg/L paclobutrazol.
After the analysis of all factors is integrated, the results of example 1(100mg/L naphthylacetic acid +200mg/L paclobutrazol) and example 9(200mg/L naphthylacetic acid +400mg/L paclobutrazol) show that the soil diameter and chlorophyll content promotion effects are good, and the root growth promotion effect is good, so that the method can be applied to actual production and plays an effective seedling strengthening role.
In addition, in order to verify the difference in effect brought by the application of the agent A and the agent B to different parts of the apocarya seedlings, the optimal combination of the agent A and the agent B obtained above is subjected to the following tests:
testing one: reference is made essentially to example 1, except that: spraying the solution A on true leaves of the carya illinoensis seedlings until the leaves drip water, and spraying the solution B on roots of the carya illinoensis seedlings until root soil is completely soaked;
and (2) testing: reference is made essentially to example 1, except that: simultaneously spraying the solution A and the solution B on true leaves of the carya illinoensis seedlings until the leaves drip water;
and (3) testing: reference is made essentially to example 1, except that: and simultaneously spraying the solution A and the solution B on the roots of the carya illinoensis seedlings until the root soil is completely soaked.
And refer to the detection mode of the spray treatment part in example 1.
TABLE 3 growth index of Carya illinoensis seedlings at different concentration ratios of naphthylacetic acid to paclobutrazol in the composition
TABLE 4 variation and growth rate of the length of the main root of Carya illinoensis seedling at different concentration ratios of naphthylacetic acid and paclobutrazol in the composition
Compared with tables 1 and 2, after the tests of the first, second and third, the overall significant decline trend appears, which is particularly shown in the aspects of main root length, seedling height, ground diameter, chlorophyll, leaf area, leaf width, leaf length and the like of the primary seedling (seedling). Therefore, only when the agent A and the agent B are sprayed to the proper positions on the apocarya seedlings in the application, the ground diameter, the chlorophyll content and the root growth can be well promoted.
While the invention has been described in further detail in connection with specific embodiments thereof, it will be understood that the invention is not limited thereto, and that various other modifications and substitutions may be made by those skilled in the art without departing from the spirit of the invention, which should be considered to be within the scope of the invention as defined by the appended claims.
Claims (7)
1. The composition for inducing the growth of the apocarya seedlings is characterized by comprising the following components in parts by weight: the composition for inducing the growth of the apocarya seedlings consists of an agent A and an agent B;
wherein the agent A is a naphthylacetic acid solution, the agent B is a paclobutrazol solution, and the concentration ratio of the agent A to the agent B is 0.5: 1;
the concentration of the naphthylacetic acid solution is 100mg/L and the concentration range of the paclobutrazol solution is 200mg/L,
alternatively, the first and second electrodes may be,
the concentration range of the naphthylacetic acid solution is 200mg/L and the concentration range of the paclobutrazol solution is 400 mg/L.
2. A preparation for inducing the growth of apocarya seedlings is characterized in that: the composition is prepared from the composition in claim 1 and auxiliary agents, and the preparation form is any one of suspending agent, missible oil and aqueous emulsion.
3. The apocarya seedling growth-inducing preparation according to claim 2, characterized in that: the formulation is in the form of a suspension.
4. Use of a composition according to claim 1 for inducing the growth of carya illinoensis seedlings.
5. Use of a formulation according to claim 2 or 3 for inducing the growth of seedlings of carya illinoensis.
6. Use of a formulation according to claim 5 for inducing the growth of seedlings of carya illinoensis, characterized in that: the agent A is sprayed on the roots of the carya illinoensis seedlings, and the agent B is sprayed on the true leaves of the carya illinoensis seedlings.
7. Use of the formulation of claim 6 for inducing the growth of apocarya seedlings, wherein: the carya illinoensis seedling is a seedling with a true leaf.
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