CN1118566C - Parafin structure slice dewaxing and clearing agent - Google Patents
Parafin structure slice dewaxing and clearing agent Download PDFInfo
- Publication number
- CN1118566C CN1118566C CN 00122030 CN00122030A CN1118566C CN 1118566 C CN1118566 C CN 1118566C CN 00122030 CN00122030 CN 00122030 CN 00122030 A CN00122030 A CN 00122030A CN 1118566 C CN1118566 C CN 1118566C
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- CN
- China
- Prior art keywords
- agent
- whiteruss
- aviation kerosene
- sherwood oil
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention discloses a structural paraffin section dewaxing and clearing agent prepared from raw materials of chemically pure liquid paraffin, analytically pure petroleum ether and aviation kerosene. The agent replaces currently used xylene solution, the raw materials contained in the agent have the advantages of convenient source, low price and low toxicity, and the use effects of the agent are similar to those of the xylene solution. When the agent is used, pathology workers' work conditions can be changed, and the health level can be increased.
Description
Technical field the present invention relates to a kind of reagent, is used for pathology educational circles, specifically a kind of tissue paraffin section de-waxing, transparent base.
Background technology is present, the solution that is used for tissue paraffin section de-waxing is xylene solution, it has the good dewaxing and the transparency, but xylene solution has moderate toxicity, xylene steam is more than 1000 * 10-6, except the injury mucous membrane, stimulate the respiratory tract, also present excitement, anesthetic action, even cause the hemorrhagic pulmonary emphysema and cause death.Long-term contact xylene solution can influence the human body hemopoietic system, causes aplastic anemia and leukemia, simultaneously the female animal reproductive system of hero is had a significant effect, and price is also than higher.
The tissue paraffin section de-waxing that a kind of hypotoxicity, price are low, result of use is good, the transparent base that provide at the deficiency of xylene solution is provided summary of the invention.
The concrete technical scheme that realizes the object of the invention is: a kind of tissue paraffin section de-waxing, transparent base are the solution with the preparation of raw material one-tenth of following volume ratio:
Chemical pure whiteruss 10-20%
Analytical pure sherwood oil 10-20%
Aviation kerosene 60-80%
The present invention is formulated with following method:
(1), gets chemical pure whiteruss, boiling point at 90-120 ℃ analytical pure sherwood oil, aviation kerosene;
(2), get a container, at room temperature pour chemical pure whiteruss, analytical pure sherwood oil, aviation kerosene into container successively respectively according to the above ratio;
(3), fully mix liquid, make the liquid uniform mixing with stirring tool;
(4), mixed uniformly liquid is put into encloses container, stand-by.
Several raw material sources among the present invention are convenient, and cost is low, hypotoxicity, and result of use of the present invention is identical with xylene solution, uses it can change pathology worker's working conditions, improves health conditions.
Specific implementation is an example to prepare 1000 ml solns, get 100 milliliters of chemical pure whiterusss, 100 milliliters of boiling points at the analytical pure sherwood oil of 90-120 degree Celsius, 800 milliliters of aviation kerosenes, stir by successively pouring in the container successively, make the liquid uniform mixing, mixed uniformly liquid is put into encloses container, stand-by.
Claims (2)
1, a kind of tissue paraffin section de-waxing, transparent base is characterized in that it is the solution that becomes with the preparation of raw material of following volume ratio:
Chemical pure whiteruss 10-20%
Analytical pure sherwood oil 10-20%
Aviation kerosene 60-80%
2, dewaxing according to claim 1, transparent base is characterized in that it being formulated with following method:
(1), gets chemical pure whiteruss, boiling point at 90-120 ℃ analytical pure sherwood oil and aviation kerosene;
(2), get a container, at room temperature pour chemical pure whiteruss, analytical pure sherwood oil, aviation kerosene into container successively respectively according to the above ratio;
(3), fully mix liquid, make the liquid uniform mixing with stirring tool;
(4), mixed uniformly liquid is put into encloses container, stand-by.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00122030 CN1118566C (en) | 2000-08-03 | 2000-08-03 | Parafin structure slice dewaxing and clearing agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00122030 CN1118566C (en) | 2000-08-03 | 2000-08-03 | Parafin structure slice dewaxing and clearing agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1337462A CN1337462A (en) | 2002-02-27 |
| CN1118566C true CN1118566C (en) | 2003-08-20 |
Family
ID=4589038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00122030 Expired - Fee Related CN1118566C (en) | 2000-08-03 | 2000-08-03 | Parafin structure slice dewaxing and clearing agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1118566C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183387B (en) * | 2011-01-21 | 2013-04-10 | 哈尔滨格林标本技术开发有限公司 | Benzene-free environment-friendly tissue or slice clearing liquid |
| WO2012147965A1 (en) * | 2011-04-28 | 2012-11-01 | 独立行政法人理化学研究所 | Method for making biological material transparent and use thereof |
| JP6044900B2 (en) | 2011-05-20 | 2016-12-14 | 国立研究開発法人理化学研究所 | Clarification reagent for biological materials and use thereof |
| WO2015022883A1 (en) | 2013-08-14 | 2015-02-19 | 独立行政法人理化学研究所 | Composition for preparing biomaterial with excellent light-transmitting property, and use thereof |
| CN104655460B (en) * | 2013-11-25 | 2017-09-12 | 无锡市江原实业技贸总公司 | A kind of transparent dewaxing liquid of pathological tissue and preparation method thereof |
| CN104155160A (en) * | 2014-08-12 | 2014-11-19 | 陆可望 | Tissue dewaxing transparent agent free of benzene |
| CN107858174B (en) * | 2017-11-23 | 2020-04-28 | 成都艾伟孚生物科技有限公司 | Special embryo culture mineral oil for in vitro fertilization and preparation method thereof |
-
2000
- 2000-08-03 CN CN 00122030 patent/CN1118566C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1337462A (en) | 2002-02-27 |
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| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |