CN111849824A - 一种提质型微生物菌剂及其制备方法 - Google Patents
一种提质型微生物菌剂及其制备方法 Download PDFInfo
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- CN111849824A CN111849824A CN202010745223.6A CN202010745223A CN111849824A CN 111849824 A CN111849824 A CN 111849824A CN 202010745223 A CN202010745223 A CN 202010745223A CN 111849824 A CN111849824 A CN 111849824A
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- parts
- bacteria
- dry powder
- lactic acid
- microbial
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 241000894006 Bacteria Species 0.000 claims abstract description 97
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 72
- 239000000843 powder Substances 0.000 claims abstract description 45
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 230000004151 fermentation Effects 0.000 claims abstract description 43
- 239000004310 lactic acid Substances 0.000 claims abstract description 36
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 36
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 34
- 239000000287 crude extract Substances 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 229920001661 Chitosan Polymers 0.000 claims abstract description 24
- 150000004676 glycans Chemical class 0.000 claims abstract description 24
- 238000002156 mixing Methods 0.000 claims abstract description 24
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 24
- 239000005017 polysaccharide Substances 0.000 claims abstract description 24
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 22
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 22
- 239000011591 potassium Substances 0.000 claims abstract description 22
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 22
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 20
- 239000002131 composite material Substances 0.000 claims abstract description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 18
- 240000008042 Zea mays Species 0.000 claims abstract description 18
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 18
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 18
- 235000005822 corn Nutrition 0.000 claims abstract description 18
- 239000008103 glucose Substances 0.000 claims abstract description 18
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 17
- 229920002401 polyacrylamide Polymers 0.000 claims abstract description 17
- 241000589151 Azotobacter Species 0.000 claims abstract description 16
- 239000002054 inoculum Substances 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 7
- 241001148471 unidentified anaerobic bacterium Species 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 6
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 6
- 239000010452 phosphate Substances 0.000 claims abstract description 6
- 230000003381 solubilizing effect Effects 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 16
- 229910052698 phosphorus Inorganic materials 0.000 claims description 16
- 239000011574 phosphorus Substances 0.000 claims description 16
- 239000011259 mixed solution Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 9
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 244000291564 Allium cepa Species 0.000 claims description 3
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims description 3
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 3
- 229920001491 Lentinan Polymers 0.000 claims description 3
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 3
- 239000001506 calcium phosphate Substances 0.000 claims description 3
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 3
- 235000011010 calcium phosphates Nutrition 0.000 claims description 3
- 229940115286 lentinan Drugs 0.000 claims description 3
- 239000004571 lime Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 7
- 239000002689 soil Substances 0.000 abstract description 19
- 239000003337 fertilizer Substances 0.000 abstract description 13
- 230000001105 regulatory effect Effects 0.000 abstract description 5
- 230000001276 controlling effect Effects 0.000 abstract description 3
- NGWKGSCSHDHHAJ-YPFQVHCOSA-N Liquoric acid Chemical compound C1C[C@H](O)C(C)(C)C2CC[C@@]3(C)[C@]4(C)C[C@H]5O[C@@H]([C@](C6)(C)C(O)=O)C[C@@]5(C)[C@@H]6C4=CC(=O)C3[C@]21C NGWKGSCSHDHHAJ-YPFQVHCOSA-N 0.000 abstract description 2
- NGWKGSCSHDHHAJ-UHFFFAOYSA-N Liquoric acid Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CC5OC(C(C6)(C)C(O)=O)CC5(C)C6C4=CC(=O)C3C21C NGWKGSCSHDHHAJ-UHFFFAOYSA-N 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 description 4
- 238000009472 formulation Methods 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
实施例1 | 实施例2 | 实施例3 | 实施例4 | 实施例5 | |
壳聚糖 | 10份 | 20份 | 13份 | 15份 | 17份 |
葡萄糖 | 10份 | 50份 | 20份 | 30份 | 40份 |
多糖粗提物干粉 | 50份 | 100份 | 60份 | 70份 | 80份 |
玉米浆干粉 | 100份 | 200份 | 130份 | 150份 | 170份 |
复合内共生芽孢杆菌 | 10份 | 20份 | 13份 | 15份 | 17份 |
酵母菌 | 20份 | 50份 | 30份 | 35份 | 40份 |
乳酸菌 | 20份 | 50份 | 30份 | 35份 | 40份 |
固氮菌 | 10份 | 20份 | 13份 | 15份 | 17份 |
解钾菌 | 20份 | 50份 | 30份 | 35份 | 40份 |
解磷菌 | 20份 | 50份 | 30份 | 35份 | 40份 |
聚丙烯酰胺 | 5份 | 10份 | 7份 | 8份 | 9份 |
酵母菌发酵液 | 100份 | 150份 | 110份 | 130份 | 140份 |
乳酸菌发酵液 | 100份 | 150份 | 110份 | 130份 | 140份 |
Claims (8)
Priority Applications (1)
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CN202010745223.6A CN111849824A (zh) | 2020-07-29 | 2020-07-29 | 一种提质型微生物菌剂及其制备方法 |
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CN202010745223.6A CN111849824A (zh) | 2020-07-29 | 2020-07-29 | 一种提质型微生物菌剂及其制备方法 |
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CN111849824A true CN111849824A (zh) | 2020-10-30 |
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CN202010745223.6A Pending CN111849824A (zh) | 2020-07-29 | 2020-07-29 | 一种提质型微生物菌剂及其制备方法 |
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CN (1) | CN111849824A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113337421A (zh) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | 一种复合微生物菌剂及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101538173A (zh) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | 一种微生物复合菌肥的生产方法 |
US20160366892A1 (en) * | 2015-05-01 | 2016-12-22 | Indigo Agriculture, Inc. | Designed complex endophyte compositions and methods for improved plant traits |
CN107082696A (zh) * | 2017-05-15 | 2017-08-22 | 福建白水农夫农业股份有限公司 | 一种有机肥料及其制备方法 |
CN108165272A (zh) * | 2017-12-26 | 2018-06-15 | 北京尚华生态环保科技股份公司 | 一种微生物农田土壤修复剂及其修复方法 |
-
2020
- 2020-07-29 CN CN202010745223.6A patent/CN111849824A/zh active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101538173A (zh) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | 一种微生物复合菌肥的生产方法 |
US20160366892A1 (en) * | 2015-05-01 | 2016-12-22 | Indigo Agriculture, Inc. | Designed complex endophyte compositions and methods for improved plant traits |
CN107082696A (zh) * | 2017-05-15 | 2017-08-22 | 福建白水农夫农业股份有限公司 | 一种有机肥料及其制备方法 |
CN108165272A (zh) * | 2017-12-26 | 2018-06-15 | 北京尚华生态环保科技股份公司 | 一种微生物农田土壤修复剂及其修复方法 |
Non-Patent Citations (1)
Title |
---|
顾威 等, 中国环境科学出版社 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113337421A (zh) * | 2021-05-06 | 2021-09-03 | 广东丽豪生物农业有限公司 | 一种复合微生物菌剂及其应用 |
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Address after: Floor 3, unit 7, building 1, Henan National University Science and Technology Park (East District), 289 West Third Ring Road, Zhengzhou high tech Industrial Development Zone, 450000 Henan Province Applicant after: Henan Haochuang Agricultural Technology Group Co.,Ltd. Address before: Floor 3, unit 7, building 1, Henan National University Science and Technology Park (East District), 289 West Third Ring Road, Zhengzhou high tech Industrial Development Zone, 450000 Henan Province Applicant before: ZHENGZHOU HAOCHUANG AGRICULTURAL TECHNOLOGY Co.,Ltd. |
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Application publication date: 20201030 |