CN111849749B - High-efficient piece-rate system of adult toy cardiac muscle cell - Google Patents
High-efficient piece-rate system of adult toy cardiac muscle cell Download PDFInfo
- Publication number
- CN111849749B CN111849749B CN202010561950.7A CN202010561950A CN111849749B CN 111849749 B CN111849749 B CN 111849749B CN 202010561950 A CN202010561950 A CN 202010561950A CN 111849749 B CN111849749 B CN 111849749B
- Authority
- CN
- China
- Prior art keywords
- digestive enzyme
- perfusion
- pipe
- enzyme liquid
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
Abstract
The invention relates to an efficient separating system for adult small animal myocardial cells, which comprises a support frame, and a perfusion liquid branch, a digestive enzyme liquid branch, a three-way valve, a perfusion main path, an exhaust mechanism and a constant temperature mechanism which are arranged on the support frame; the perfusion liquid branch and the digestive enzyme liquid branch are communicated with the perfusion main path through a three-way valve, the constant temperature mechanism is arranged on the perfusion liquid branch and the digestive enzyme liquid branch and used for controlling the temperature of the perfusion liquid and the digestive enzyme liquid, and the exhaust mechanism is respectively communicated with the perfusion liquid branch and the digestive enzyme liquid branch and used for exhausting bubbles in the perfusion liquid branch and the digestive enzyme liquid branch. Compared with the prior art, the device has the advantages of simple structure, simple and convenient control method, easy operation and the like.
Description
Technical Field
The invention belongs to the technical field of living cell separation systems, and relates to an efficient separation system for adult small animal myocardial cells.
Background
The primary cultured adult myocardial cells are widely used in the research processes of cardiovascular disease mechanisms, cardiovascular drug development and myocardial toxicology screening. The perfusion separation system of the existing adult myocardial cell separation system of small animals mainly comprises two or more than two glass water bath thermostats, peristaltic pumps and circulating water bath pots (DL Nature Life sciences, TYPE 803; Radnoti,120107 EZ; Shanghai Orkets, ALC-M). Because the existing adult myocardial cell separation system comprises two or more serially connected glass water bath thermostats, the initial perfusion liquid and the digestive enzyme liquid sequentially enter the heart through the same pipeline, the accurate control of the crossing time between different liquids is very difficult, and meanwhile, the system cannot be used independently of a peristaltic pump, so that the investment cost is increased.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide an efficient separation system for adult small animal myocardial cells, which is used for solving the problem that the final separation effect is influenced because the crossing time between initial perfusion liquid and digestive enzyme liquid is difficult to accurately control in the separation experiment process of the myocardial cells.
The purpose of the invention can be realized by the following technical scheme:
an efficient separating system for adult small animal myocardial cells comprises a support frame, and a perfusion liquid branch, a digestive enzyme liquid branch, a three-way valve, a perfusion main path, an exhaust mechanism and a constant temperature mechanism which are arranged on the support frame;
the perfusion liquid branch and the digestive enzyme liquid branch are communicated with the perfusion main path through a three-way valve;
the constant temperature mechanism is arranged on the perfusion liquid branch and the digestive enzyme liquid branch and is used for controlling the temperature of the perfusion liquid and the digestive enzyme liquid;
the exhaust mechanism is respectively communicated with the perfusion liquid branch and the digestive enzyme liquid branch and is used for exhausting bubbles in the perfusion liquid branch and the digestive enzyme liquid branch.
The perfusion liquid branch or the digestive enzyme liquid branch is controlled to be communicated with the perfusion main path through the three-way valve, so that the accurate control of the switching time between the perfusion liquid and the digestive enzyme liquid is realized, and the problem that the last separation effect is poor due to the influence of too long or too short supply time of the digestive enzyme liquid on the myocardial cells is solved.
Further, the three-way valve is a double-inclined-hole piston. The double-inclined-hole piston is rotated to switch the liquid flowing into the main filling path.
Furthermore, the perfusion liquid branch comprises a perfusion liquid storage tank, a perfusion liquid inlet pipe and a perfusion liquid outlet pipe which are sequentially communicated;
the perfusion liquid outlet pipe is communicated with a three-way valve;
the perfusion liquid inlet pipe and the perfusion liquid outlet pipe are both arranged in the constant temperature mechanism.
Furthermore, the digestive enzyme liquid branch comprises a digestive enzyme liquid storage tank, a digestive enzyme liquid inlet pipe and a digestive enzyme liquid outlet pipe which are sequentially communicated;
the digestive enzyme liquid outlet pipe is communicated with a three-way valve;
the digestive enzyme liquid inlet pipe and the digestive enzyme liquid outlet pipe are both arranged in the constant temperature mechanism.
And connecting hoses are respectively arranged between the bottom outlet of the perfusion liquid storage tank and the lower end inlet of the perfusion liquid inlet pipe and between the bottom outlet of the digestive enzyme liquid storage tank and the lower end inlet of the digestive enzyme liquid inlet pipe, so that the height of the perfusion liquid storage tank and the height of the digestive enzyme liquid storage tank can be conveniently adjusted.
Furthermore, the perfusion liquid inlet pipe is spirally wound outside the perfusion liquid outlet pipe; the digestive enzyme liquid inlet pipe is spirally wound outside the digestive enzyme liquid outlet pipe.
The perfusion liquid inlet pipe and the digestive enzyme liquid inlet pipe are spirally arranged to increase the contact area of the perfusion liquid and the digestive enzyme liquid with the constant temperature mechanism, so that the effect of effectively controlling the temperature is achieved.
Furthermore, the exhaust mechanism comprises a perfusion liquid exhaust pipe arranged between the perfusion liquid inlet pipe and the perfusion liquid outlet pipe, a perfusion liquid exhaust pipe communicated with the perfusion liquid exhaust pipe, a digestive enzyme liquid exhaust pipe arranged between the digestive enzyme liquid inlet pipe and the digestive enzyme liquid outlet pipe, and a digestive enzyme liquid exhaust pipe communicated with the digestive enzyme liquid exhaust pipe.
As a preferable technical scheme, the perfusion liquid emptying pipe is communicated with the top of the perfusion liquid exhaust pipe, and the digestive enzyme liquid emptying pipe is communicated with the top of the digestive enzyme liquid exhaust pipe.
Preferably, the height of the outlet of the perfusion liquid emptying pipe is not lower than the liquid level in the perfusion liquid storage tank, and the height of the outlet of the digestive enzyme liquid emptying pipe is not lower than the liquid level in the digestive enzyme liquid storage tank.
Furthermore, the perfusion liquid exhaust pipe is respectively communicated with the upper end of the perfusion liquid inlet pipe and the upper end of the perfusion liquid outlet pipe;
the digestive enzyme liquid exhaust pipe is respectively communicated with the upper end of the digestive enzyme liquid inlet pipe and the upper end of the digestive enzyme liquid outlet pipe.
The perfusate or digestive enzyme liquid flowing out of the liquid inlet pipe enters the corresponding liquid outlet pipe through the exhaust pipe, a certain liquid level height is kept in the exhaust pipe, bubbles wrapped and carried in the perfusate or digestive enzyme are crushed from the liquid level and are discharged from the emptying pipe together with residual air in the pipeline, and therefore the bubbles are prevented from entering the main perfusion path to influence the experiment.
Furthermore, the constant temperature mechanism comprises a constant temperature pipe and a constant temperature water bath kettle which is circularly communicated with the constant temperature pipe;
the perfusate liquid inlet pipe, the perfusate liquid outlet pipe, the perfusate exhaust pipe, the digestive enzyme liquid inlet pipe, the digestive enzyme liquid outlet pipe and the digestive enzyme liquid exhaust pipe are all arranged in the constant temperature pipe.
And a circulating heat exchange pipeline is arranged between the constant temperature pipe and the constant temperature water bath, the constant temperature pipe and a heat exchange medium in the constant temperature water bath circulate through the circulating heat exchange pipeline, and the heat exchange medium in the constant temperature pipe is wrapped outside the perfusion liquid inlet pipe, the perfusion liquid outlet pipe, the perfusion liquid exhaust pipe, the digestive enzyme liquid inlet pipe, the digestive enzyme liquid outlet pipe and the digestive enzyme liquid exhaust pipe, so that the effects of controlling the temperature of the perfusion liquid and the digestive enzyme liquid are achieved.
Furthermore, the main perfusion path comprises a connecting pipe, a liquid outlet valve and an injection needle which are sequentially communicated;
the connecting pipe is communicated with the three-way valve;
an experimental bottle is arranged below the injection needle.
Furthermore, a circulating peristaltic pump is arranged between the experiment bottle and the digestive enzyme liquid storage tank;
and circulating hoses are arranged between the circulating peristaltic pump and the experimental bottle and between the circulating peristaltic pump and the digestive enzyme liquid storage tank and are respectively communicated through the circulating hoses.
The working principle is as follows: perfusate or digestive enzyme in the perfusate storage tank or the digestive enzyme storage tank respectively enters a spiral perfusate liquid inlet pipe or a digestive enzyme liquid inlet pipe through a corresponding connecting hose and flows into a corresponding exhaust pipe from bottom to top, bubbles wrapped by the perfusate or the digestive enzyme liquid escape from the spiral perfusate liquid inlet pipe or the digestive enzyme liquid inlet pipe and are discharged from the exhaust pipe, then the perfusate or the digestive enzyme flows to a three-way valve through a corresponding liquid outlet pipe and is controlled to switch to enter a medium of a perfusion main path by rotating the three-way valve, and the medium entering the perfusion main path is injected into the heart for the experiment through an injection needle so as to carry out the subsequent experiment for separating the myocardial cells of the adult small animals.
Compared with the prior art, the invention has the following characteristics:
1) the invention integrates the water bath thermostats of two spiral liquid inlet pipes, so that initial perfusion liquid and digestive enzyme liquid enter a system through different spiral pipes, and finally, the switching time between the perfusion liquid and the digestive enzyme liquid is accurately controlled by rotating a double-inclined-hole piston close to the heart for experiments;
2) the perfusion speed can be controlled by adjusting the heights of the perfusion liquid storage tank and the digestive enzyme liquid storage tank or the rotation angle of the double-inclined-hole piston, and the control method is simple and convenient and easy to operate;
3) the circulating peristaltic pump can be used independently, so that the cost is reduced;
4) according to the invention, the heating devices for heating the perfusion liquid branch, the digestive enzyme liquid branch and other pipelines are integrated in the thermostatic tube containing the double spiral tubes, so that the problem of temperature control consistency of the solution is solved, the trouble that each container or pipeline needs to be provided with the heating device is avoided, the cost of the device is reduced, and the convenience of building the device is improved;
5) the perfusion main path has a simple structure, so that on one hand, the constant temperature tube for heating is close to the heart for the experiment, the perfusion main path heating device additionally used for ensuring the temperature control problem in the conventional perfusion device is avoided, on the other hand, the flow in the perfusion main path is favorably shortened, the perfusion liquid passing through the three-way valve can directly and quickly reach the heart for the experiment, the problem that the experiment accuracy is influenced by the delay of the solution switching time due to the excessive residual liquid in the pipeline is avoided, and the accurate and quick solution switching effect is favorably realized;
5) according to the invention, the perfusate or digestive enzyme liquid flowing out of the liquid inlet pipe enters the corresponding liquid outlet pipe through the exhaust pipe, and the diameter of the exhaust pipe is larger than that of the liquid inlet pipe and the liquid outlet pipe, so that the perfusate or digestive enzyme liquid is kept at a certain liquid level height in the exhaust pipe, and bubbles entrapped in the perfusate or digestive enzyme liquid are broken and dissipated from the liquid level, and are discharged from the emptying pipe together with air remained in the pipeline, thereby avoiding the problem that the separation experiment of the myocardial cells is influenced because the bubbles enter the main perfusion path.
Drawings
FIG. 1 is a schematic diagram of a high efficiency separation system for cardiomyocytes in an adult small animal according to the present invention;
the notation in the figure is:
1-perfusate branch, 101-perfusate storage tank, 102-perfusate inlet pipe, 103-perfusate outlet pipe, 2-digestive enzyme liquid branch, 201-digestive enzyme liquid storage tank, 202-digestive enzyme liquid inlet pipe, 203-digestive enzyme liquid outlet pipe, 3-perfusion main pipe, 301-connecting pipe, 302-liquid outlet valve, 303-injection needle and 4-exhaust mechanism, 401-perfusate exhaust pipe, 402-perfusate evacuation pipe, 403-digestive enzyme liquid exhaust pipe, 404-digestive enzyme liquid evacuation pipe, 5-constant temperature mechanism, 501-constant temperature pipe, 502-constant temperature water bath, 503-circulation heat exchange pipeline, 6-circulation peristaltic pump, 7-circulation hose, 8-connection hose, 9-support frame and 10-three-way valve.
Detailed Description
The invention is described in detail below with reference to the figures and specific embodiments. The present embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, but the scope of the present invention is not limited to the following embodiments.
Example (b):
as shown in fig. 1, the efficient separating system for adult small animal myocardial cells comprises a support frame 9, and a perfusion liquid branch 1, a digestive enzyme liquid branch 2, a three-way valve 10, a perfusion main path 3, an exhaust mechanism 4 and a constant temperature mechanism 5 which are arranged on the support frame 9;
the perfusion liquid branch 1 comprises a perfusion liquid storage tank 101, a perfusion liquid inlet pipe 102 and a perfusion liquid outlet pipe 103 which are sequentially communicated, wherein the perfusion liquid inlet pipe 102 is spirally wound outside the perfusion liquid outlet pipe 103; the digestive enzyme liquid branch 2 comprises a digestive enzyme liquid storage tank 201, a digestive enzyme liquid inlet pipe 202 and a digestive enzyme liquid outlet pipe 203 which are sequentially communicated, wherein the digestive enzyme liquid inlet pipe 202 is spirally wound outside the digestive enzyme liquid outlet pipe 203, a connecting hose 8 is further respectively arranged between the bottom outlet of the perfusate storage tank 101 and the lower end inlet of the perfusate liquid inlet pipe 102 and between the bottom outlet of the digestive enzyme liquid storage tank 201 and the lower end inlet of the digestive enzyme liquid inlet pipe 202, and the connecting hose 8 is a silicone tube with the inner diameter of 4 mm.
The exhaust mechanism 4 comprises a perfusion fluid exhaust pipe 401 respectively communicated with an upper end outlet of the perfusion fluid inlet pipe 102 and an upper end inlet of the perfusion fluid outlet pipe 103, a perfusion fluid exhaust pipe 402 communicated with the top of the perfusion fluid exhaust pipe 401, a digestive enzyme fluid exhaust pipe 403 respectively communicated with an upper end outlet of the digestive enzyme fluid inlet pipe 202 and an upper end inlet of the digestive enzyme fluid outlet pipe 203, and a digestive enzyme fluid exhaust pipe 404 communicated with the top of the digestive enzyme fluid exhaust pipe 403, wherein the perfusion fluid exhaust pipe 402 and the digestive enzyme fluid exhaust pipe 404 are silicone tubes with the inner diameter of 8 mm.
The constant temperature mechanism 5 comprises a constant temperature pipe 501, a constant temperature water bath 502 circularly communicated with the constant temperature pipe 501, and a circulating heat exchange pipeline 503 arranged between the constant temperature pipe 501 and the constant temperature water bath 502; the circulating heat exchange pipeline 503 is a silicone tube with an inner diameter of 10mm, and the perfusate liquid inlet pipe 102, the perfusate liquid outlet pipe 103, the perfusate gas outlet pipe 401, the digestive enzyme liquid inlet pipe 202, the digestive enzyme liquid outlet pipe 203 and the digestive enzyme liquid outlet pipe 403 are all arranged in the constant temperature pipe 501.
Perfusion main path 3 is including connecting pipe 301, drain valve 302 and syringe needle 303 that communicate in proper order, syringe needle 303 below is equipped with experiment bottle 11, be equipped with circulation peristaltic pump 6 between experiment bottle 11 and digestive enzyme liquid storage tank 201, all be equipped with circulation hose 7 between circulation peristaltic pump 6 and experiment bottle 11 and the digestive enzyme liquid storage tank 201, and be linked together through circulation hose 7 respectively, wherein connecting pipe 301 is the silicone tube that the external diameter is 10mm, experiment bottle 11 is 100mL beaker, be equipped with digestive enzyme liquid in the beaker, circulation hose 7 is the silicone tube, drain valve 302 is medical tee bend, be used for discharging the drain pipe before the experiment begins, the bubble in tee bend 10 and the connecting pipe, and the start and the closure of digestive enzyme liquid and perfusion liquid transportation process.
The three-way valve 10 is a double-inclined-hole piston and is respectively communicated with the outlet end of the perfusion liquid outlet pipe 103, the outlet end of the digestive enzyme liquid outlet pipe 203 and the inlet end of the connecting pipe 301.
The working principle is as follows: openings are formed in the tops of the perfusate storage tank 101 and the digestive enzyme liquid storage tank 201, perfusate or digestive enzyme liquid in the perfusate storage tank 101 or the digestive enzyme liquid storage tank 201 respectively enters the spiral perfusate liquid inlet pipe 102 or the digestive enzyme liquid inlet pipe 202 through the corresponding connecting hose 8 under the action of gravity, and flows into the corresponding exhaust pipe from bottom to top, bubbles wrapped by the perfusate or the digestive enzyme liquid escape from the spiral perfusate liquid inlet pipe and are discharged from the exhaust pipe, then the perfusate or the digestive enzyme flows to the position of the three-way valve 10 through the corresponding liquid outlet pipe, the medium entering the main perfusion path 3 is controlled and switched by rotating the three-way valve 10, and the medium entering the main perfusion path 3 is injected into the aorta of the heart for the experiment through the injection needle 303 so as to perform the subsequent experiment of separating the cardiac muscle cells of the adult small animals.
The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (4)
1. An efficient separating system for adult small animal myocardial cells is characterized by comprising a support frame (9), and a perfusion liquid branch (1), a digestive enzyme liquid branch (2), a three-way valve (10), a perfusion main path (3), an exhaust mechanism (4) and a constant temperature mechanism (5) which are arranged on the support frame (9);
the perfusion liquid branch (1) comprises a perfusion liquid storage tank (101), a perfusion liquid inlet pipe (102) and a perfusion liquid outlet pipe (103) which are communicated in sequence; the perfusion liquid inlet pipe (102) is spirally wound outside the perfusion liquid outlet pipe (103), and the perfusion liquid outlet pipe (103) is communicated with the three-way valve (10);
the perfusion liquid inlet pipe (102) and the perfusion liquid outlet pipe (103) are both arranged in the constant temperature mechanism (5);
the digestive enzyme liquid branch (2) comprises a digestive enzyme liquid storage tank (201), a digestive enzyme liquid inlet pipe (202) and a digestive enzyme liquid outlet pipe (203) which are communicated in sequence; the digestive enzyme liquid inlet pipe (202) is spirally wound outside the digestive enzyme liquid outlet pipe (203), and the digestive enzyme liquid outlet pipe (203) is communicated with the three-way valve (10);
the digestive enzyme liquid inlet pipe (202) and the digestive enzyme liquid outlet pipe (203) are both arranged in the constant temperature mechanism (5); the three-way valve (10) is also communicated with the main perfusion path (3);
the exhaust mechanism is respectively communicated with the perfusion liquid branch (1) and the digestive enzyme liquid branch (2) and is used for exhausting bubbles in the perfusion liquid branch (1) and the digestive enzyme liquid branch (2);
the exhaust mechanism (4) comprises a perfusion liquid exhaust pipe (401) arranged between a perfusion liquid inlet pipe (102) and a perfusion liquid outlet pipe (103), a perfusion liquid exhaust pipe (402) communicated with the perfusion liquid exhaust pipe (401), a digestive enzyme liquid exhaust pipe (403) arranged between a digestive enzyme liquid inlet pipe (202) and a digestive enzyme liquid outlet pipe (203), and a digestive enzyme liquid exhaust pipe (404) communicated with the digestive enzyme liquid exhaust pipe (403);
the perfusate exhaust pipe (401) is respectively communicated with the upper end of the perfusate liquid inlet pipe (102) and the upper end of the perfusate liquid outlet pipe (103);
the digestive enzyme liquid exhaust pipe (403) is respectively communicated with the upper end of the digestive enzyme liquid inlet pipe (202) and the upper end of the digestive enzyme liquid outlet pipe (203);
the constant temperature mechanism (5) is arranged on the perfusion liquid branch (1) and the digestive enzyme liquid branch (2) and used for controlling the temperature of the perfusion liquid and the digestive enzyme liquid, and comprises a constant temperature pipe (501) and a constant temperature water bath kettle (502) which is circularly communicated with the constant temperature pipe (501);
the perfusate liquid inlet pipe (102), the perfusate liquid outlet pipe (103), the perfusate gas outlet pipe (401), the digestive enzyme liquid inlet pipe (202), the digestive enzyme liquid outlet pipe (203) and the digestive enzyme liquid outlet pipe (403) are all arranged in the constant temperature pipe (501).
2. The efficient adult small-animal cardiomyocyte separation system according to claim 1, wherein the three-way valve (10) is a double inclined-hole piston.
3. The efficient adult small animal cardiomyocyte separation system according to claim 1, wherein the main perfusion path (3) comprises a connecting tube (301), a liquid outlet valve (302) and an injection needle (303) which are sequentially communicated;
the connecting pipe (301) is communicated with the three-way valve (10);
an experimental bottle (11) is arranged below the injection needle (303).
4. The efficient adult small animal myocardial cell separation system according to claim 3, characterized in that a circulating peristaltic pump (6) is arranged between the experimental bottle (11) and the digestive enzyme liquid storage tank (201);
and circulating hoses (7) are respectively arranged between the circulating peristaltic pump (6) and the experimental bottle (11) and between the circulating peristaltic pump and the digestive enzyme liquid storage tank (201), and are respectively communicated through the circulating hoses (7).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2020102474296 | 2020-03-31 | ||
| CN202010247429 | 2020-03-31 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111849749A CN111849749A (en) | 2020-10-30 |
| CN111849749B true CN111849749B (en) | 2022-02-18 |
Family
ID=72987640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010561950.7A Active CN111849749B (en) | 2020-03-31 | 2020-06-18 | High-efficient piece-rate system of adult toy cardiac muscle cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111849749B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565652A (en) * | 2003-06-24 | 2005-01-19 | 中国人民解放军第三军医大学 | Simple extraneous liver perfusion device for digesting and separating liver cells |
| WO2017152343A1 (en) * | 2016-03-07 | 2017-09-14 | 浙江大学 | Recirculating perfusion bioreactor device that can realize three-dimensional scaffold recirculating perfusion |
| WO2020004088A1 (en) * | 2018-06-28 | 2020-01-02 | 株式会社Ｓｃｒｅｅｎホールディングス | Perfusion device and perfusion method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201293783Y (en) * | 2008-10-13 | 2009-08-19 | 武汉大学 | Device for circulation and perfusion of in-vitro heart of laboratory animal |
| CN202421174U (en) * | 2012-01-30 | 2012-09-05 | 徐州医学院 | Isolated heart perfusion device |
| CN203807461U (en) * | 2014-05-16 | 2014-09-03 | 成都泰盟软件有限公司 | Buffer and perfusion system |
| CN105018332A (en) * | 2015-07-17 | 2015-11-04 | 徐州医学院 | Cell perfusion separation device |
| CN109280610B (en) * | 2018-09-19 | 2023-09-19 | 贵州医科大学附属医院 | Automatic batch separation adult mouse myocardial cell perfusion system |
| CN110786317A (en) * | 2019-11-04 | 2020-02-14 | 广州市冠流生物医学科技有限公司 | Integrated organ perfusion apparatus |
-
2020
- 2020-06-18 CN CN202010561950.7A patent/CN111849749B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1565652A (en) * | 2003-06-24 | 2005-01-19 | 中国人民解放军第三军医大学 | Simple extraneous liver perfusion device for digesting and separating liver cells |
| WO2017152343A1 (en) * | 2016-03-07 | 2017-09-14 | 浙江大学 | Recirculating perfusion bioreactor device that can realize three-dimensional scaffold recirculating perfusion |
| WO2020004088A1 (en) * | 2018-06-28 | 2020-01-02 | 株式会社Ｓｃｒｅｅｎホールディングス | Perfusion device and perfusion method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111849749A (en) | 2020-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101732770B (en) | Dual-cavity liquid storage tank for biological artificial liver support system | |
| CN111849749B (en) | High-efficient piece-rate system of adult toy cardiac muscle cell | |
| CN201821759U (en) | Trickling water supply-type aquatic creature cultivation system for toxicological experiment | |
| CN205981851U (en) | Clear up system in succession with adjustable pressure, flow | |
| CN102038980A (en) | Manual preparation device of kidney dialysis liquid | |
| CN205556644U (en) | Separation heart perfusion device suitable for rat | |
| CN206404756U (en) | A kind of multi-function outer circulation kettle system | |
| CN211004668U (en) | Purified water pipeline sterilization system | |
| CN108678922A (en) | A kind of medical treatment antiseptic solution feeding device | |
| CN211435112U (en) | Novel medicinal concentrator | |
| CN209347600U (en) | A kind of bath of centralized intelligence chemical drug is for medicine and control system | |
| CN209498252U (en) | Egg system is washed in a kind of hatching of heat exchange type | |
| CN209160180U (en) | Online filling buffer decontamination system | |
| CN212548312U (en) | Liquid medicine concentrator | |
| CN213141890U (en) | Large-volume protein renaturation device | |
| CN206488515U (en) | A kind of reheater for absorption heat pump | |
| CN208586288U (en) | A kind of microculture experiment heating oxygen supply device | |
| CN220897736U (en) | Simple and easy isolated heart perfusion device | |
| CN209726870U (en) | A kind of heat reclaim unit for Chinese medicine extractor | |
| US11202972B2 (en) | Automatic tritium extraction device and method for environmental monitoring | |
| CN217459415U (en) | Efficient tissue organ acellular scaffold elution perfusion system | |
| CN212818227U (en) | Cold trap device with external circulation cold-carrying system | |
| CN216550479U (en) | Perfusion type culture device | |
| CN209188547U (en) | A kind of bio-pharmaceuticals vortice | |
| CN217392393U (en) | Efficient carboxylic acid configuration system for ABS resin production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |