CN111840130A - After-sun repair composition and application thereof - Google Patents

Abstract

The invention discloses a post-basking repair composition and application thereof, wherein the post-basking repair composition comprises cannabidiol, borneol and oat beta-glucan, and further comprises auxiliary synergistic components of a radix sileris extract and a calendula extract. The after-sun repair composition provided by the application is mild in formula design, has a synergistic effect after compounding of the components, and reduces the irritation of the product while enhancing the effect, so that the cannabidiol achieves the most obvious repair effect under the combined action of other effective components. The composition has a remarkable instantaneous repairing effect after being used for a skin care product and is very promising in market prospect.

Description

After-sun repair composition and application thereof

Technical Field

The invention belongs to the technical field of daily cosmetics, and particularly relates to a post-basking repair composition and application thereof.

Background

The epidermis layer and the dermis layer of the skin mainly comprise keratinocytes and fibroblasts, UVA in ultraviolet rays has extremely strong penetrating power and can penetrate into the dermis layer of the skin through the epidermis layer of the skin, and UVB has weaker penetrating power than UVA, most of UVB is absorbed by the epidermis layer, and a small part of UVB reaches the dermis layer. The phenomena of pigmentation of black pigment, sunburn, skin dehydration and aging, inflammation, reddening and burning and the like can be caused after long-time exposure to ultraviolet rays, the skin immunity is seriously reduced, skin cancer or pathological changes and the like, more and more sunscreen products appear in the market in succession, but the sunscreen skin care product cannot play a role for a long time all day, the action time is generally about 2-6 hours, the sunscreen effect can not be completely achieved, and the problems of skin burden, pore blockage and the like can be easily caused after the sunscreen skin care product is applied too much. Therefore, the instantaneous repair after the sun is a key for overcoming various problems of the skin after the sun is sunned, 6-8 hours after the sun is sunned is the gold time for repair, and the sun-cured repair product can be reasonably and effectively used for rapidly relieving the problems of the skin after the sun is sunned.

Cannabidiol (CBD) is one of the most important non-addictive components in plants, has multiple pharmacological activities of spasm resistance, inflammation resistance, anxiety resistance, oxidation resistance, damage repair and the like, CBD cosmetics with multiple efficacies are on the market at present, and a small amount of reports are made in the sun protection aspect, for example, patent CN 109820786A discloses a sun cream capable of repairing skin damage, and the sun cream achieves the sun protection and repair effects by combining an industrial big fried dough twist leaf extract with a physical sun-screening agent and a chemical sun-screening agent, but when CBD is used alone, the transdermal absorption effect is poor, the repair effect is not obvious, and the after-sun repair effect cannot be achieved.

Disclosure of Invention

In order to solve the technical problems, the invention provides the after-sun repair composition and the preparation method thereof.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

in one aspect of the invention, a post-basking repair composition is provided, comprising cannabidiol, borneol and oat beta-glucan.

Optionally, the mass ratio of cannabidiol, borneol and oat beta-glucan is 0.5-15:0.1-1: 1-30.

The Cannabidiol (CBD) source may be a chemical synthesis product, a biosynthetic product, a botanical industrial cannabis extract, or prepared in other ways; preferably, the phytocannabidiol of the present application is an extract of industrial Cannabis, which may be the stem, flower, leaf, root and/or seed coat of industrial Cannabis sativa l, preferably industrial Cannabis flower and/or leaf; the dosage is calculated by cannabidiol pure substance.

The industrial hemp extract contains 30-99% of cannabidiol by mass percentage;

preferably, the industrial hemp extract contains 50 to 99% of cannabidiol by mass percentage, more preferably 60 to 99% of cannabidiol by mass percentage, and particularly preferably 95 to 99% of cannabidiol by mass percentage.

Specifically, in the mass ratio of cannabidiol to borneol to oat beta-glucan, the lower limit of the proportion of cannabidiol can be independently selected from 0.5, 2.5, 3, 5 and 6; the upper limit of the cannabidiol proportion may be independently selected from 8, 10, 12, 13, 15.

The lower limit of the proportion of borneol can be independently selected from 0.1, 0.2, 0.3, 0.4 and 0.5; the upper limit of the proportion of borneol can be independently selected from 0.6, 0.7, 0.8, 0.9 and 1.

The lower limit of the proportion of oat beta-glucan can be independently selected from 1, 5, 10, 18 and 20; the upper limit of the proportion of oat beta-glucan can be independently selected from 21, 23, 25, 27 and 30.

Optionally, the after-sun repair composition further comprises an auxiliary synergistic ingredient;

preferably, the auxiliary synergistic component comprises at least one of radix Saposhnikoviae root extract and herba Sidae Rhombifoliae extract;

preferably, the mass ratio of the radix sileris extract to the cannabidiol is 1-20: 0.5-15;

preferably, the mass ratio of the marigold extract to the cannabidiol is 1-20: 0.5-15.

Specifically, in the mass ratio of the radix sileris extract to the cannabidiol, the lower limit of the proportion of the radix sileris extract can be independently selected from 1, 2.5, 3, 4 and 5; the upper limit of the proportion of the radix sileris extract can be independently selected from 8, 10, 15, 18 and 20.

The lower limit of the cannabidiol ratio may be independently selected from 0.5, 2.5, 3, 5, 6; the upper limit of the cannabidiol proportion may be independently selected from 8, 10, 12, 13, 15.

Specifically, in the mass ratio of the calendula extract to cannabidiol, the lower limit of the ratio of the calendula extract can be independently selected from 1,3, 4, 4.5 and 8; the upper limit of the ratio of the calendula extract can be independently selected from 10, 13, 15, 18 and 20.

The lower limit of the cannabidiol ratio may be independently selected from 0.5, 2.5, 3, 5, 6; the upper limit of the cannabidiol proportion may be independently selected from 8, 10, 12, 13, 15.

Optionally, the after-sun repair composition further comprises hydrolyzed sclerotium rolfsii gum, wherein the mass ratio of the hydrolyzed sclerotium rolfsii gum to the cannabidiol is 1-10: 0.5-15.

Specifically, in the mass ratio of the sclerotium rolfsii gum to the cannabidiol, the lower limit of the proportion of the sclerotium rolfsii gum is independently selected from 1, 2.5, 3, 4 and 5; the upper limit of the proportion of the hydrolyzed sclerotium rolfsii gum can be independently selected from 6, 7, 8, 9 and 10.

The lower limit of the cannabidiol ratio may be independently selected from 0.5, 2.5, 3, 5, 6; the upper limit of the cannabidiol proportion may be independently selected from 8, 10, 12, 13, 15.

Optionally, the after-sun repair composition further comprises chamomile essential oil, wherein the mass ratio of the chamomile essential oil to the cannabidiol is 0.1-1: 0.5-15.

Specifically, in the mass ratio of the chamomile essential oil to the cannabidiol, the lower limit of the proportion of the chamomile essential oil can be independently selected from 0.1, 0.2, 0.3, 0.4 and 0.5; the upper limit of the proportion of the chamomile essential oil can be independently selected from 0.6, 0.7, 0.8, 0.9 and 1.

The lower limit of the cannabidiol ratio may be independently selected from 0.5, 2.5, 3, 5, 6; the upper limit of the cannabidiol proportion may be independently selected from 8, 10, 12, 13, 15.

The applicant in the application creatively finds that the combination of borneol and oat beta-glucan has a synergistic effect on the transdermal absorption of cannabidiol, can remarkably improve the transdermal absorption effect of cannabidiol, enhances the bioavailability of cannabidiol, contributes to better exerting the effect, and further improves the activities of antioxidation, after-sun repair and the like of cannabidiol. The plant extract essence radix sileris extract and the marigold extract have synergistic effect in the aspects of oxidation resistance, after-sun repair and the like after being combined with cannabidiol; after the sclerotium rolfsii and the chamomile essential oil are added, the effects of relieving and moisturizing the skin can be improved, and the irritation of the product can be reduced. The composition is mild in formula, has a synergistic effect after the components are matched, can relieve skin pathogenic heat and toxic heat, enhances the after-sun repair effect of the cannabidiol, reduces irritation while enhancing the effect of the components, enables the cannabidiol to achieve the most obvious repair effect under the combined action of other effective components, saves cost, and greatly utilizes the activities of oxidation resistance, after-sun repair and the like of the cost ratio of the cannabidiol.

In another aspect, the present application provides a post-sun repair skin care product comprising at least one of any of the above post-sun repair compositions.

The composition can be used for preparing after-sun repair skin care products such as after-sun repair essence, emulsion, cream and facial mask.

Optionally, the after-sun repair skin care product comprises the after-sun repair composition in an amount of 0.1-10 wt%.

Specifically, in the after-sun repair skin care product, the lower limit of the content of the after-sun repair composition can be independently selected from 0.1 wt%, 1 wt%, 2 wt%, 4 wt% and 5 wt%; the upper limit of the content of the after-sun repair composition can be independently selected from 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%.

Optionally, the after-sun repair skin care product is a facial mask;

preferably, the content of the after-sun repair composition in the mask solution of the mask is 0.1 wt% to 10 wt%.

Specifically, the lower limit of the content of the post-basking repair composition in the facial mask solution can be independently selected from 0.1 wt%, 0.5 wt%, 1.0 wt%, 1.5 wt%, 2.04 wt%, 2.14 wt%, 2.2 wt%, 3 wt%; the upper limit of the content of the post-basking repair composition may be independently selected from 3.07 wt%, 3.28 wt%, 4 wt%, 4.58 wt%, 5.22 wt%, 5.6 wt%, 5.72 wt%, 10 wt%.

Optionally, the facial mask solution further comprises at least one of a humectant, a thickener, a stabilizer, an emollient, an emulsifier, an antioxidant, and a preservative. The above materials are all common adjuvants for facial mask.

Optionally, the humectant is at least one selected from sodium hyaluronate, 1, 3-butanediol, dipropylene glycol, 1, 2-hexanediol, glycerol, sodium polyglutamate, betaine, alpha-mannan, trehalose, and propylene glycol, and the amount is 10-20 wt%;

the thickener is at least one of hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, carbomer, sodium acrylate, celluloses, glyceryl acrylate/acrylic acid copolymer/PVM/MA copolymer, gellan gum, AVC (ammonium acryloyldimethyl taurate/VP copolymer) and xanthan gum, and the dosage is 0.5-2 wt%;

the stabilizer is at least one of PEG-400, PEG-200, PEG-1000 and PEG-800, and the dosage is 0.5-1.5 wt%;

the softening agent is at least one of bis-PEG-18 methyl ether dimethylsilane, PEG-12 polydimethylsiloxane, PEG-7 glyceryl cocoate, PEG-6 caprylic/capric glycerides, Hojoba wax PEG-120 ester and DOSIL 2511 (bis-PEG-18 methyl ether dimethylsilane), and the dosage is 0.3-0.8 wt%;

the emulsifier is at least one selected from coconut oil alcohol polyether-7, PPG-1-PEG-9 lauryl glycol ether, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, polysorbate-20, polysorbate-60, and is used in an amount of 0.5-2 wt%;

the antioxidant is at least one selected from tocopherol acetate, diethylhexyl syringylidenemalonate, 3-O-ethyl ascorbic acid, ascorbyl glucoside, magnesium ascorbyl phosphate, ascorbyl palmitate, and butylated hydroxytoluene, and is used in an amount of 0.5-2 wt%;

the antiseptic is at least one of p-hydroxyacetophenone, phenoxyethanol, ethylhexyl glycerol, methyl hydroxybenzoate, propyl hydroxybenzoate, methylisothiazolinone, potassium sorbate and sodium benzoate, and is used in an amount of 0.1-0.6 wt%.

Specifically, the lower limit of the amount of the humectant may be independently selected from 10 wt%, 11 wt%, 12 wt%, 13 wt%, 15 wt%; the upper limit of the amount of humectant may be independently selected from 15.4 wt%, 17 wt%, 18 wt%, 19 wt%, 20 wt%.

Specifically, the lower limit of the amount of the thickener can be independently selected from 0.5 wt%, 0.7 wt%, 0.9 wt%, 1 wt%, 1.1 wt%; the upper limit of the amount of the thickener can be independently selected from 1.35 wt%, 1.5 wt%, 1.6 wt%, 1.8 wt%, 2 wt%.

Specifically, the lower limit of the amount of the stabilizer may be independently selected from 0.5 wt%, 0.6 wt%, 0.7 wt%, 8 wt%, 9 wt%; the upper limit of the amount of the stabilizer may be independently selected from 1 wt%, 1.1 wt%, 1.2 wt%, 1.4 wt%, 1.5 wt%.

Specifically, the lower limit of the amount of the softening agent can be independently selected from 0.3 wt%, 0.35 wt%, 0.4 wt%, 0.45 wt%, 0.5 wt%; the upper limit of the amount of the softening agent can be independently selected from 0.55 wt%, 0.6 wt%, 0.7 wt%, 0.75 wt%, 0.8 wt%.

Specifically, the lower limit of the amount of the emulsifier can be independently selected from 0.5 wt%, 0.7 wt%, 0.9 wt%, 1 wt%, 1.1 wt%; the upper limit of the amount of the emulsifier can be independently selected from 1.2 wt%, 1.4 wt%, 1.5 wt%, 1.7 wt%, 2.0 wt%.

Specifically, the lower limit of the amount of the antioxidant may be independently selected from 0.5 wt%, 0.7 wt%, 0.9 wt%, 1 wt%, 1.1 wt%; the upper limit of the amount of the antioxidant may be independently selected from 1.35 wt%, 1.5 wt%, 1.6 wt%, 1.8 wt%, 2 wt%.

Specifically, the lower limit of the amount of the preservative can be independently selected from 0.1 wt%, 0.15 wt%, 0.2 wt%, 0.25 wt%, 0.3 wt%; the upper limit of the amount of the preservative can be independently selected from 0.35 wt%, 0.4 wt%, 0.45 wt%, 0.5 wt%, 0.6 wt%.

Optionally, the preparation method of the facial mask liquid at least comprises the following steps:

s001 and preparation of A phase:

adding cannabidiol, antioxidant and emulsifier into appropriate amount of humectant, dissolving in 50-60 deg.C water bath, cooling to 30-40 deg.C, adding Borneolum Syntheticum and flos Matricariae Chamomillae essential oil, stirring to dissolve, adding the other part of humectant and water, and stirring to obtain cannabidiol microemulsion as phase A;

preparation of S002 and B phases:

mixing the rest humectant, hydrolyzed Sclerotinia sclerotiorum, thickener, stabilizer, emollient, antiseptic and water, heating to 80-90 deg.C, swelling for 25-30min, and cooling to below 45 deg.C to obtain phase B.

S003, mixing the phase B with the phase A, and homogenizing to obtain a main phase;

in the present application, the homogenization conditions are: 6000-.

S004, adding the divaricate saposhnikovia root extract, the calendula extract and the oat beta-glucan into the main phase, and uniformly stirring to obtain the after-sun repairing mask liquid.

Preferably, in the step S001, the humectant is an alcohol humectant, and the amount of the alcohol humectant is 40-60 wt% of the total amount of the humectant;

preferably, the alcohol humectant is at least one of dipropylene glycol, butylene glycol, propylene glycol and glycerol.

Specifically, the amount of the alcohol humectant added in the two steps in step 1 is not particularly limited, and the alcohol humectant added in the first step is only required to be capable of dissolving cannabidiol, antioxidant and emulsifier, and the remaining alcohol humectant is added in the second step.

Specifically, the lower limit of the amount of the alcohol humectant in the total amount of the humectant can be independently selected from 40 wt%, 42 wt%, 45 wt%, 47 wt% and 50 wt%; the upper limit of the amount of the alcohol humectant in the total amount of the humectant can be independently selected from 52 wt%, 53 wt%, 55 wt%, 57 wt% and 60 wt%.

The beneficial effect of this application lies in:

the applicant in the application creatively finds that the combination of borneol and oat beta-glucan has a synergistic effect on the transdermal absorption of cannabidiol, can remarkably improve the transdermal absorption of cannabidiol, enhance the bioavailability of cannabidiol, contribute to better exertion of effects, and further improve the activities of antioxidation, after-sun repair and the like of cannabidiol. And the plant extract essence radix sileris extract and the marigold extract have synergistic effect in the aspects of oxidation resistance, after-sun repair and the like after being compatible with cannabidiol, so that the repair, whitening and other effects can be obviously improved, the soothing and moisturizing effect can be improved after the hydrolyzed sclerotium rolfsii and the chamomile essential oil are added, and the irritation of the product is reduced. The composition is mild in formula, has a synergistic effect by optimizing the proportion, can relieve skin pathogenic heat and toxic heat, enhances the after-sun repair effect of cannabidiol, reduces irritation while enhancing the effect by matching the components, achieves the most obvious repair effect by the combined action of other effective components of cannabidiol, saves cost, and greatly utilizes the activities of oxidation resistance, after-sun repair and the like of the gold cannabidiol with a price ratio. The composition has a remarkable instantaneous repairing effect after being used for a skin care product and has a great market potential.

Drawings

FIG. 1 is a graph showing ROS clearance after UV irradiation of HaCaT human keratinocytes with examples and comparative examples.

Detailed Description

The invention will be further illustrated with reference to the following specific examples.

The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, materials and the like used in the following examples are commercially available unless otherwise specified.

Cannabidiol adopted in the embodiment of the application is CBD with the purity of 99%, and a manufacturer is Yunan Han union pharmaceutical Co., Ltd;

the radix sileris extract and the calendula extract are self-extracted by an ultrasonic alcohol extraction method:

extracting radix Saposhnikoviae and radix Hamamelis mollis with 70% ethanol water solution at 60 deg.C under ultrasonic for 20min, recovering under reduced pressure, and freeze drying to obtain extract

Example 1

The preparation raw materials and the addition amount of the after-sun repairing mask liquid are as follows according to mass percentage:

the after-sun repair composition is: cannabidiol 1.5%, oat beta-glucan 0.5%, borneol 0.04%;

humectant: 2% of sodium hyaluronate (added in the form of sodium hyaluronate stock solution, wherein the mass concentration of the sodium hyaluronate in the sodium hyaluronate stock solution is 1%), 5% of 1, 3-butanediol, 3% of dipropylene glycol, 0.4% of 1, 2-hexanediol, 4% of glycerol and 1% of propylene glycol;

the thickening agent is: 1% of Gemba gum (GRANHYDROGEL, GA-NP) and 0.35% of AVC (Acryloyldimethyl ammonium taurate/VP copolymer);

the stabilizer is: 1% of PEG-400 (polyethylene glycol-400);

the softening agent comprises: DOWSIL 2511 (bis-PEG-18 methyl ether dimethylsilane) 0.5%;

the emulsifier is as follows: polysorbate-601.5%, PEG-60 hydrogenated castor oil 0.5%;

the antioxidant is: 0.1% of tocopherol acetate and 0.5% of 3-O-ethyl ascorbic acid;

the preservative is as follows: 0.4 percent of p-hydroxyacetophenone.

The total of the components is 100 percent, and the deficiency is filled with water.

The preparation method of the mask liquid comprises the following steps:

1. preparation of phase A: preparation of cannabidiol microemulsion

Adding cannabidiol, tocopherol acetate, 3-O-ethyl ascorbic acid, 1% of PEG-60 hydrogenated castor oil and 1% of polysorbate-60 into dipropylene glycol, dissolving in water bath at 50 ℃, cooling to 30 ℃, adding borneol, stirring for dissolving, continuously adding 1, 3-butanediol and water, and stirring uniformly to obtain cannabidiol microemulsion as phase A;

2. preparation of phase B:

mixing sodium hyaluronate, glycerol, propylene glycol, 1,2 hexanediol, Gerber gum, AVC, PEG-400, bis-PEG-18 methyl ether dimethylsilane, p-hydroxyacetophenone and water, heating to 80 deg.C in water bath, swelling for 30min, and cooling to below 45 deg.C to obtain phase B.

3. Mixing B phase with A phase, and homogenizing at 7000rpm for 5min to obtain main phase

4. Adding the oat beta-glucan into the main phase, and uniformly stirring to obtain the post-basking repairing mask liquid. Designated sample 1 and the mask obtained using sample 1, designated M1.

Example 2

The preparation raw materials and the addition amount of the after-sun repairing mask liquid are as follows according to mass percentage:

post-basking repair composition: cannabidiol 0.25%, ledebouriella root extract 0.25%, calendula extract 1.5%, oat beta-glucan 0.1%, borneol 0.1%, the other additives are the same as in example 1, the total of the above components is 100%, and the shortage is filled with water.

The preparation method of the mask liquid comprises the following steps:

1. preparation of phase A: preparation of cannabidiol microemulsion

Adding cannabidiol, tocopherol acetate, 3-O-ethyl ascorbic acid, 1% of PEG-60 hydrogenated castor oil and 1% of polysorbate-60 into dipropylene glycol, dissolving in water bath at 60 ℃, cooling to 40 ℃, adding borneol, stirring for dissolving, continuously adding 1, 3-butanediol and water, and stirring uniformly to obtain cannabidiol microemulsion as phase A;

2. preparation of phase B:

mixing sodium hyaluronate, glycerol, propylene glycol, 1, 2-hexanediol, Gerber gum, AVC, PEG-400, bis-PEG-18 methyl ether dimethylsilane, p-hydroxyacetophenone and water, heating to 90 deg.C in water bath, swelling for 25min, and cooling to below 45 deg.C to obtain phase B.

3. Mixing the phase B with the phase A, and homogenizing at 6000rpm for 8 minutes to obtain a main phase;

4. adding radix Saposhnikoviae extract, herba Sidae Rhombifoliae extract, and herba Avenae Fatuae beta-dextran into the main phase, and stirring to obtain after-sun repairing facial mask solution. Designated sample 2 and the mask obtained using sample 2, designated M2.

Example 3

The preparation raw materials and the addition amount of the after-sun repairing mask liquid are as follows according to mass percentage:

post-basking repair composition: cannabidiol 0.6%, ledebouriella root extract 0.5%, calendula extract 0.45%, oat beta-glucan 1.5%, borneol 0.02%, the other additives are the same as in example 1, the total of the above components is 100%, and the shortage is filled with water.

The preparation method of the facial mask liquid is the same as that of the facial mask liquid in example 2. The resulting post-basking repair mask solution was designated as sample 3 and the mask obtained using sample 3 was designated as M3.

Example 4

The preparation raw materials and the addition amount of the after-sun repair mask are as follows:

post-basking repair composition: cannabidiol 0.05%, ledebouriella root extract 2%, calendula extract 1.5%, oat beta-glucan 1%, borneol 0.03%; the other addition amounts were the same as in example 1, and the total of the above components was 100%, and the shortage was made up with water.

The preparation method of the facial mask liquid is the same as that of the facial mask liquid in example 2. The resulting post-basking repair mask solution was designated as sample 4 and the mask obtained using sample 4 was designated as M4.

Example 5

The preparation raw materials and the addition amount of the after-sun repairing mask liquid are as follows according to mass percentage:

post-basking repair composition: cannabidiol 0.3%, ledebouriella root extract 0.4%, calendula extract 0.45%, sclerotium rolfsii gum 0.1%, oat beta-glucan 2%, borneol 0.03%; the other addition amounts were the same as in example 1, and the total of the above components was 100%, and the shortage was made up with water.

The preparation method of the mask liquid comprises the following steps:

1. preparation of phase A: preparation of cannabidiol microemulsion

Adding cannabidiol, tocopherol acetate, 3-O-ethyl ascorbic acid, 1% of PEG-60 hydrogenated castor oil and 1% of polysorbate-60 into dipropylene glycol, dissolving in 55 ℃ water bath, cooling to 35 ℃, adding borneol, stirring for dissolving, continuously adding 1, 3-butanediol and water, and stirring uniformly to obtain cannabidiol microemulsion which is phase A;

2. preparation of phase B:

mixing sodium hyaluronate, glycerol, propylene glycol, 1, 2-hexanediol, Gerber gum, AVC, PEG-400, bis-PEG-18 methyl ether dimethylsilane, p-hydroxyacetophenone, hydrolyzed sclerotium rolfsii gum and water, heating to 85 deg.C, swelling for 26min, and cooling to below 45 deg.C to obtain phase B.

3. Mixing the phase B with the phase A, and homogenizing at 8000rpm for 4 minutes to obtain a main phase;

4. adding radix Saposhnikoviae extract, herba Sidae Rhombifoliae extract, and herba Avenae Fatuae beta-dextran into the main phase, and stirring to obtain after-sun repairing facial mask solution. Designated sample 5 and the mask obtained using sample 5, designated M5.

EXAMPLE 6

The preparation raw materials and the addition amount of the after-sun repair mask are as follows:

post-basking repair composition: cannabidiol 1.5%, ledebouriella root extract 0.1%, marigold extract 0.1%, oat beta-glucan 3%, borneol 0.02%, sclerotium rolfsii gum 1%, the other additives are the same as in example 1, the total of the above components is 100%, and the deficiency is compensated by water.

The preparation method of the facial mask solution is the same as that of example 5. The resulting post-basking repair mask solution was designated sample 6 and the mask obtained using sample 6 was designated M6.

Example 7

The preparation raw materials and the addition amount of the after-sun repair mask are as follows:

post-basking repair composition: cannabidiol 0.05%, hydrolyzed sclerotium rolfsii gum 0.1%, radix sileris extract 1.5%, calendula officinalis extract 2%, chamomile essential oil 0.1%, oat beta-glucan 1.8%, borneol 0.05%, the rest of the addition is the same as that of the example 1, the total of the above components is 100%, and the deficiency is filled with water.

The preparation method of the mask liquid comprises the following steps:

1. preparation of phase A: preparation of cannabidiol microemulsion

Adding cannabidiol, tocopherol acetate, 3-O-ethyl ascorbic acid, 1% of PEG-60 hydrogenated castor oil and 1% of polysorbate-60 into dipropylene glycol, dissolving in 57 ℃ water bath, cooling to 32 ℃, adding borneol and chamomile essential oil, stirring for dissolving, continuously adding 1, 3-butanediol and water, and stirring uniformly to obtain cannabidiol microemulsion which is phase A;

2. preparation of phase B:

mixing sodium hyaluronate, glycerol, propylene glycol, 1, 2-hexanediol, Gerber gum, AVC, PEG-400, bis-PEG-18 methyl ether dimethylsilane, p-hydroxyacetophenone, hydrolyzed sclerotium rolfsii gum and water, heating to 82 deg.C, swelling for 28min, and cooling to below 45 deg.C to obtain phase B.

3. Mixing the phase B with the phase A, and homogenizing at 6500rpm for 7 minutes to obtain a main phase;

4. adding radix Saposhnikoviae extract, herba Sidae Rhombifoliae extract, and herba Avenae Fatuae beta-dextran into the main phase, and stirring to obtain after-sun repairing facial mask solution. Designated sample 7 and the mask obtained using sample 7, designated M7.

Example 8

The preparation raw materials and the addition amount of the after-sun repair mask are as follows according to the mass percentage:

post-basking repair composition: cannabidiol 0.3%, ledebouriella root extract 0.3%, calendula extract 0.4%, chamomile essential oil 0.05%; 0.25% of hydrolyzed sclerotium rolfsii gum, 0.8% of oat beta-glucan, 0.04% of borneol and the balance of additives are the same as in example 1, the total amount of the components is 100%, and the deficiency is filled with water.

The facial mask solution was prepared as in example 7. The resulting post-basking repair mask solution was designated as sample 8 and the mask obtained using sample 8 was designated as M8.

Example 9

The preparation raw materials and the addition amount of the after-sun repair mask are as follows according to the mass percentage:

post-basking repair composition: cannabidiol 1.5%, ledebouriella root extract 0.3%, calendula extract 0.3%, chamomile essential oil 0.01%; 0.1% of hydrolyzed sclerotium rolfsii gum, 3% of oat beta-glucan and 0.01% of borneol, and the rest of the components are the same as the components in the example 1, wherein the total amount of the components is 100%, and the deficiency is filled with water.

The facial mask solution was prepared as in example 7. The resulting post-basking repair mask solution was designated as sample 9 and the mask obtained using sample 9 was designated as M9.

Comparative example 1

The after-sun repair composition is: cannabidiol 1.5%, the balance of adjuvant ingredients are the same as in example 1. The preparation method was the same as example 1, and the obtained mask was designated as M12.

Comparative example 2

Post-basking repair composition: cannabidiol 1.5%, borneol 0.1%, and the other auxiliary components are the same as in example 1, the preparation method is the same as in example 1, and the obtained facial mask is marked as M13.

Comparative example 3

The after-sun repair composition is: cannabidiol 1.5%, oat beta-glucan 3%, and the balance of adjuvant ingredients were the same as in example 1, the preparation method was the same as in example 1, and the obtained mask was designated as M14.

Comparative example 4

The after-sun repair composition is: the preparation method of the mask is the same as that of the mask in the embodiment 2, and the mask is marked as M15, wherein the mask comprises 2% of the divaricate saposhnikovia root extract, 2% of the calendula officinalis extract, 1% of the oat beta-glucan, 0.03% of the borneol and the balance of auxiliary ingredients, and the auxiliary ingredients are the same as those in the embodiment 1.

1. Mask performance index determination

(1) Appearance: a pale yellow translucent emulsion.

(2) pH value: weakly acidic 5.5.

(3) Heat resistance test: the temperature is reduced to normal temperature after 24 hours without oil analysis phenomenon at 45 +/-2 ℃ in an electric heating constant temperature culture phase.

(4) Cold resistance test: the temperature is returned to normal temperature after 24 hours in a refrigerator at-8 +/-2 ℃ without oil-water analysis.

When the performance of the sample M1-M9 obtained by the method is measured, 9 samples meet the detection requirements.

2. Skin irritation test of facial mask

According to the acute skin irritation test method of the technical Specification for safety of cosmetics, 9 groups of facial mask liquid obtained in the preparation implementation column are respectively set to be 1-9 groups, 4 white rabbits are correspondingly selected for each group, and adult, healthy and skin-free conditions are required. The hair on both sides of the spine of the experimental animal is cut off about 24 hours before the experiment, the epidermis cannot be damaged, and the hair removing range is about 3cm multiplied by 3cm on the left and the right respectively. Approximately 0.5mL of the test article is applied directly to the skin and covered with two layers of gauze (2.5 cm. times.2.5 cm) and a layer of cellophane or the like, and secured with a non-irritating adhesive and bandage. The other side of the skin served as a control. The application time is 2h by adopting a sealing test. After the test is finished, the residual test substance is removed by warm water. After the test substances are removed, the skin reaction of the smearing part is observed for 1, 24, 48 and 72 hours to see whether the phenomena of erythema, hyperemia, pallor and swelling and erosion appear. The results of the stimulation experiments are reported in table 1. The obtained facial mask solutions of 9 groups have no skin irritation.

TABLE 1 skin irritation test results of facial mask

Note: "0" means no signs; "1" indicates a mild sign; "2" indicates light signs; "3" indicates a heavy sign.

3. Transdermal absorption test

The experimental method comprises the following steps: fixing the pretreated skin of the back of porket between the supply pool and the receiving pool of TK-12D type transdermal diffusion tester (the receiving pool has a volume of 8ml and an effective permeation area of 3 cm)²) The receiving pool is filled with 7ml of physiological saline solution, 1ml of buffer solution is added into the liquid taking pipe, the magnetic stirring rotor matched with the stirring is placed in the absorption pool, the whole device is placed in a constant temperature water bath (36 ℃ plus or minus 1 ℃) constant temperature water area, and the magnetic stirring rotating speed is started to be 200 rpm/min. After the water bath is kept at a constant temperature, 5ml of test sample solution is added into a supply pool (9 groups of facial mask solutions obtained in the embodiment of the application are prepared into 1-9 groups respectively, two groups of domestic industrial hemp leaf after-sun repair facial mask patches containing industrial hemp leaf extraction components on the market are prepared into 10 and 11 groups (marked as samples M10 and M11) and 3 groups of facial mask solutions obtained in comparative examples 1-3 are prepared into 12-14 groups respectively, and 14 groups of facial mask solutions are counted in total)Sampling 100 mul in a receiving pool after 2, 4 and 6 hours, placing the sampling in a 1.5ml EP tube, adding 900 mul of ethanol with the mass fraction of 75 percent for dissolving and shaking, standing for 10min, centrifuging at 12000r/min for 15 min, taking supernate, passing the supernate through a 0.45 mu m nylon membrane, and using the content of cannabidiol as an investigation index for standby high performance liquid detection;

chromatographic conditions are as follows: the detection wavelength is 220nm, the flow rate is 1ml/min, the sample injection volume is 10 mul, the mobile phase is acetonitrile-water (volume ratio is 7: 3), the column temperature is 30 +/-0.5 ℃, the chromatographic column C18 (150mm multiplied by 4.6mm, 3 mu m) is adopted, the peak area of each sample after each sampling is recorded, and the sample concentration of the detection solution is calculated.

Data processing:

and after eleven groups of samples at three different time points are respectively taken to receive liquid for pretreatment, the peak areas of the samples are measured by high performance liquid chromatography, and the accumulated permeability Q is calculated. The calculation formula is as follows:

Q＝[C_n×V₁+∑C_i×V₂]/S(i＝1...n-1)

in the formula, Q: accumulating the permeation amount; s: an effective diffusion area; v₁: the volume of the receiving pool is 8 ml; c_n: the mass concentration (mg/ml) of the receiving solution at the time of the nth sampling; c_i: the mass concentration (mg/ml) of the receiving solution at the sampling time of the ith time; v₂: the volume of each sample.

The percent diffusion was calculated by cumulant, the permeability calculation formula:

P＝P_t/P₀*100％

in the formula, P_t: the content of the sample in the receiving cell; p₀: the content of the cell sample is supplied.

The results of the data obtained from the experiments are shown in table 2.

TABLE 2 permeation rate of cannabidiol at different time points for each sample

From the experimental results in table 2, it can be seen that the permeation rates of cannabidiol in samples at different times are different, the permeation rates at two hours are not much different, and the permeation rates of the samples in each group are improved along with the increase of time, wherein the permeation rates of the M1-M9 groups are obviously higher than those of the samples M10 and M11 on the market, which indicates that the permeation effect of cannabidiol in the repairing composition is stronger than that of the samples on the market, and the permeation rate at 6 hours reaches about 9.0%, but the samples on the market are far lower than that of the repairing composition. Comparison of M12-M14 with M1 shows that the combination of borneol and oat beta-glucan has synergistic effect on the transdermal absorption of cannabidiol.

4. Post-basking skin repair test for facial mask

Ultraviolet rays are the main reasons of skin melanin deposition, free radical flooding and skin inflammation, directly damage the bottom layer of the skin, cause burning heat, peeling, pain and the like, the tests pass different proportions and match with active substances obtained in the examples, and different facial mask liquids with different maximum effect of antioxidation repair are used for carrying out the ROS elimination function of HaCaT cells (keratinocyte, main cells forming epidermis and accounting for 80 percent of epidermal cells), and the synergy of the matching of the active ingredients is judged.

The test method comprises the following steps:

(1) HaCaT human keratinocytes were inoculated in 10% fetal bovine serum and DMEM high-glucose cell culture medium containing 1% streptomycin;

(2) replacing the culture medium every three days;

(3) taking appropriate amount of cells in growth phase, according to 2 × 10⁴cfu/mL concentration was plated in 96-well cell culture plates, 1mL per well at 37 ℃ with 5% CO₂After humidified culturing for 24 hours, allowing the cells to adhere to the wall completely;

(4) preparing 9 groups of facial mask solutions obtained in the embodiment of the application and respectively setting the 9 groups of facial mask solutions as 1-9 groups, preparing two groups of domestic industrial hemp leaf post-sun repair facial mask patches containing industrial hemp leaf extract components on the market, setting 10 and 11 groups (marked as samples M10 and M11) and 4 groups of facial mask solutions obtained in comparative examples 1-4 as 12-15 groups respectively, setting 15 groups of facial mask solutions in total, protecting HaCaT human keratinocytes by the same volume of the facial mask solutions obtained in the groups 1-15, and carrying out parallel experiments on each group of facial mask solutions for 3 times;

(5) the same dose of ultraviolet radiation was used for 120 minutes;

(6) and (4) detecting the content of ROS according to the operation of the kit.

And (3) testing results:

the experimental results are shown in fig. 1, and the comparison between the sample M1 and the sample M12-M14 shows that the ROS clearance of cannabidiol is improved after adding borneol or oat β -glucan, and the comparison between the samples M13, M14 and the sample M1 shows that the effect of the combination of borneol and oat β -glucan is remarkably improved, which indicates that the combination of borneol and oat β -glucan has a synergistic effect, and the mechanism of the synergistic effect may be that the transdermal absorption of cannabidiol is synergistically promoted after the combination of borneol and oat β -glucan. Compared with the sample M2-M4, the sample M1 shows that the ROS clearance is higher after the radix sileris extract and the calendula extract are added, which indicates that the radix sileris extract, the calendula extract and cannabidiol have synergistic antioxidation and auxiliary synergistic effect after being combined; and the ROS clearance rate of the sample M1-M9 is higher than that of similar masks M10 and M11 in the market.

5. Sensory test of the product after drying in the sun for soothing and moisturizing:

1. the tested samples were: the tested samples are the facial mask liquids obtained in the groups of examples 1-9, 10-11 groups of facial mask liquids on the market and 12-15 groups of facial mask liquids obtained in the comparative example.

2. The test method comprises the following steps: randomly selecting 300 female normal volunteers without skin allergy history, averagely dividing the volunteers into 15 groups, distributing 15 groups of corresponding facial mask liquid for each group of 20 female normal volunteers according to a normal sequence, selecting summer July as a test time, using the facial mask liquid for one month, particularly using the facial mask liquid after sun drying, averagely using the facial mask liquid once every three days, and finally evaluating the using effect of the facial mask for each group, wherein the scoring standard is as follows: the score is 0-10 and is divided into five grades, and the using effect is good: 9-10 minutes; preferably: 8-9 min; generally 7 to 8 points; poor: 5-6 min; difference: 3-4 points.

3. The evaluation indexes include final repairing effect of reddening after sun exposure, relieving effect, moisturizing effect, absorption effect, calming effect after sun exposure, and skin brightening effect.

4. Using a one month post effect score, the results are shown in table 3:

and (4) conclusion: as can be seen from the table, compared with the sample M12-M14, the sample M1 of the present application has a significantly improved repair effect after sun exposure, which indicates that the combined borneol and oat β -glucan has a synergistic promotion effect on the repair effect of cannabidiol after sun exposure; compared with the sample M2-M4, the sample M1 has better repairing and skin brightening evaluation of M2-M4, which shows that after the radix sileris extract and the calendula extract are added, the repairing and skin brightening effects after the sun are better, and the synergistic effect is achieved; compared with the samples M1-M6, the samples M7-M9 have better calming, moisturizing and soothing evaluations of the samples M7-M9, which shows that the soothing and calming effects of cannabidiol can be synergistically improved by adding the sclerotium rolfsii gum and the chamomile essential oil, the irritation is reduced, and the moisturizing effect is better.

Although the present application has been described with reference to a few embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the application as defined by the appended claims.

Claims (10)

1. A post-basking repair composition comprising cannabidiol, borneol and oat beta-glucan.

2. The post-basking repair composition of claim 1, wherein the mass ratio of cannabidiol, borneol and oat beta-glucan is 0.5-15:0.1-1: 1-30.

3. The post-basking restoration composition according to claim 1, further comprising an auxiliary potentiating ingredient;

preferably, the auxiliary synergistic component comprises at least one of radix sileris extract and calendula extract;

preferably, the mass ratio of the radix sileris extract to the cannabidiol is 1-20: 0.5-15;

preferably, the mass ratio of the calendula extract to cannabidiol is 1-20: 0.5-15.

4. The sunless tanning composition of claim 1, further comprising hydrolyzed sclerotium rolfsii gum, wherein the mass ratio of hydrolyzed sclerotium rolfsii gum to cannabidiol is 1-10: 0.5-15.

Preferably, the after-sun repair composition further comprises chamomile essential oil, and the mass ratio of the chamomile essential oil to the cannabidiol is 0.1-1: 0.5-15.

5. The post-basking restoration composition according to any one of claims 1-4, wherein the source of cannabidiol may be a chemical synthesis product, a biological synthesis product, a botanical industrial cannabis extract;

preferably, the plant industrial cannabis extract is an industrial cannabis leaf extract, and the industrial cannabis extract contains 30% -99% of cannabidiol by mass percentage.

6. A post-sun repair skin care product comprising at least one of the post-sun repair compositions according to any one of claims 1 to 5.

7. The after-sun repair skin care product of claim 6, wherein the after-sun repair skin care product comprises from 0.1 to 10 wt% of the after-sun repair composition.

8. The after-sun repair skin care product of claim 6, wherein the after-sun repair skin care product is a mask;

preferably, the content of the after-sun repair composition in the mask solution of the mask is 0.1 wt% -10 wt%.

9. The after-sun repair skin care product of claim 8, wherein the mask solution further comprises: at least one of a humectant, a thickening agent, a stabilizer, a softening agent, an emulsifier, an antioxidant and a preservative;

preferably, the dosage of each component in the mask liquid is as follows: 10-20 wt% of humectant, 0.5-2 wt% of thickener, 0.5-1.5 wt% of stabilizer, 0.3-0.8 wt% of softening agent, 0.5-2 wt% of emulsifier, 0.5-2 wt% of antioxidant and 0.1-0.6 wt% of preservative.

10. The after-sun repair skin care product according to claim 9, wherein the preparation method of the mask solution at least comprises the following steps:

s001 and preparation of A phase:

adding cannabidiol, antioxidant and emulsifier into a part of humectant, dissolving in 50-60 deg.C water bath, cooling to 30-40 deg.C, adding Borneolum Syntheticum and flos Matricariae Chamomillae essential oil, stirring to dissolve, adding the other part of humectant and water, and stirring to obtain cannabidiol microemulsion as phase A;

preparation of S002 and B phases:

adding the rest humectant, hydrolyzed sclerotium rolfsii gum, thickener, stabilizer, emollient, and antiseptic into water, stirring, heating to 80-90 deg.C in water bath, swelling for 25-30min, and cooling to below 45 deg.C to obtain phase B;

s003, mixing the phase B with the phase A, and homogenizing to obtain a main phase;

s004, adding the divaricate saposhnikovia root extract, the calendula extract and the oat beta-glucan into the main phase, and uniformly stirring to obtain a post-basking repairing mask liquid;

preferably, in the step S001, the humectant is an alcohol humectant, and the amount of the alcohol humectant is 40-60 wt% of the total amount of the humectant;

preferably, the alcohol humectant is at least one of dipropylene glycol, butylene glycol, propylene glycol and glycerol.

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