CN111837962A - Water chestnut tissue culture and seed fruit sterile cultivation integrated system - Google Patents

Water chestnut tissue culture and seed fruit sterile cultivation integrated system Download PDF

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Publication number
CN111837962A
CN111837962A CN202010804435.7A CN202010804435A CN111837962A CN 111837962 A CN111837962 A CN 111837962A CN 202010804435 A CN202010804435 A CN 202010804435A CN 111837962 A CN111837962 A CN 111837962A
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China
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water
conveyor belt
support
cultivation
tray
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CN202010804435.7A
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Chinese (zh)
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丁年生
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Wuhu Xilaiwang Agricultural Technology Co ltd
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Wuhu Xilaiwang Agricultural Technology Co ltd
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Priority to CN202010804435.7A priority Critical patent/CN111837962A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B3/00Cleaning by methods involving the use or presence of liquid or steam
    • B08B3/02Cleaning by the force of jets or sprays
    • B08B3/022Cleaning travelling work

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention discloses a water chestnut tissue culture seed sterile cultivation integrated system, which comprises a sterilization pool, a first conveyor belt, a roller shaft group, a second conveyor belt, a bracket and a cultivation disc, wherein the first conveyor belt is arranged in the sterilization pool, the roller shaft group is arranged obliquely above the first conveyor belt and is parallel to the first conveyor belt, the rear end of the roller shaft group is positioned below the front end of the first conveyor belt, and a spraying device is arranged on the sterilization pool corresponding to the roller shaft group, and compared with the prior art, the water chestnut tissue culture seed sterile cultivation integrated system has the beneficial effects that: the water chestnut seeds are sterilized in a sterilizing pond, then can be driven to a roller shaft group through a conveyor belt, and are cleaned on the roller shaft group, the cleaned water chestnut seeds move forward under the driving of the roller shaft group and finally fall into a stacking frame, and the water chestnut seeds are driven to the left side to a working area by a second conveyor belt in the stacking frame; when the cultivation plate is filled, the roller can be rotated, the stacking frame is lifted or put down through the sling, so that the stacking frame and the cultivation plate are positioned on the same horizontal plane, and the water chestnut seeds can be conveniently placed by workers.

Description

Water chestnut tissue culture and seed fruit sterile cultivation integrated system
Technical Field
The invention relates to the technical field of water chestnut cultivation, in particular to a water chestnut tissue culture seed sterile cultivation integrated system.
Background
The water chestnut is bred and planted in a naturally open field and infected with virus and bacteria, so that the water chestnut is easy to get ill and weak in disease and pest resistance, further the planting yield of the water chestnut is low, the quality of the water chestnut is poor, the economic benefit of the water chestnut planting is greatly influenced, and the development of the water chestnut industry is restricted.
In order to ensure the health of the water chestnut tissue culture seed and improve the yield and the quality of water chestnut, scientific seedling culture is imperative. Therefore, the person skilled in the art provides an integrated system for sterile cultivation of water chestnut tissue culture seed to solve the problems mentioned in the background art.
Disclosure of Invention
The invention aims to provide an integrated system for aseptic cultivation of water chestnut tissue culture seeds, which aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a water chestnut group banks up aseptic integration system of cultivating of seed fruit, includes sterilization pond, first conveyer belt, roller group, second conveyer belt, support and cultivates the dish, first conveyer belt is installed in sterilization pond, and roller group installs in the oblique top of first conveyer belt, and roller group is parallel with first conveyer belt, and the rear end of roller group is located the below of first conveyer belt front end, the sterilization is eaten to correspond roller group department and is installed spray set, sterilization pond front side is provided with first support, and the second conveyer belt is installed in first support, and first support front side is provided with the second support, installs a plurality of in the second support and cultivates the dish.
As a further scheme of the invention: the inside rear side in sterilization pond is provided with the sterilization chamber, corresponds sterilization chamber front side in the sterilization pond and is provided with the washing chamber, and sterilization pond right side wall mounting has the second water pump, and the water inlet of second water pump and the right wall connection in sterilization pond, the delivery port and the spray pipe of second water pump are connected, and the spray pipe rear end is connected with the back wall in sterilization pond. .
As a still further scheme of the invention: spray set includes shower nozzle and third water pump, and the third water pump is installed on the sterilizing chamber, and the drinking-water pipe of third water pump inserts and establishes and wash the intracavity, and the outlet pipe and the shower nozzle of third water pump are connected, and the shower nozzle is installed on the antetheca in sterilizing chamber.
As a still further scheme of the invention: the automatic stacking device is characterized in that a guide rail is arranged on the first support, a stacking frame is arranged above the guide rail, a plurality of sliding blocks are arranged at the bottom of the stacking frame, the sliding blocks are erected on the guide rail, the second conveyor belt is arranged in the stacking frame, a rack is arranged at the bottom of the stacking frame, a motor is arranged in the first support, a gear is arranged on a rotating shaft of the motor, and the gear is meshed with the rack and is connected with the rack.
As a still further scheme of the invention: the supporting device comprises a first support, a plurality of supporting rods, a plurality of guide rails, a plurality of rollers, a plurality of second slings, a plurality of rollers, a plurality of supporting rods, a plurality of sliding supports, a plurality of guide rails, a plurality of pulleys, a plurality of second slings, a plurality of rollers, a plurality of second slings, a plurality of first slings, a plurality of second slings, a plurality of rollers, a plurality of.
As a still further scheme of the invention: be provided with the delivery port on the antetheca of cultivation dish, the delivery port left side is provided with the water inlet, and cultivation dish layering is installed in the second support, second support front side is provided with the culture solution case, installs first water pump on the culture solution case, and the drinking-water pipe of first water pump is inserted and is established in the culture solution incasement, fixedly connected with transfer line on the outlet pipe of first water pump, and the transfer line top is connected with the water inlet of superiors, the delivery port below is connected with the back flow, and the back flow bottom is connected rather than the water inlet of below, and the back flow bottom of lower floor is inserted and is established in the culture.
As a still further scheme of the invention: the cultivation tray comprises a fifth conveying belt, an upper tray, a lower tray, hanging lugs, a water tank and driving wheels, the driving wheels are installed at four corners of the water tank, the fifth conveying belt is sleeved on the two driving wheels in the front-back direction, the lower tray is arranged in the water tank, the upper tray is arranged above the lower tray, the upper tray and the lower tray are connected with the fifth conveying belt through hanging ropes, the hanging lugs are arranged on the left side and the right side of the water tank, and a plurality of cultivation holes are formed in the upper tray and the lower tray.
As a still further scheme of the invention: a plurality of water inlet pipes are installed in the water tank and communicated with the water inlet.
Compared with the prior art, the invention has the beneficial effects that:
1. the water chestnut seeds can be driven to the roller shaft group through the conveyor belt after being sterilized in the sterilizing tank, and are cleaned on the roller shaft group, the cleaned water chestnut seeds move forward under the driving of the roller shaft group and finally fall into the stacking frame, and the water chestnut is driven to the left side to a working area by the second conveyor belt in the stacking frame, so that the flow process is realized, and the sterilizing effect and the utilization efficiency of sterilized water are improved;
2. when the cultivation tray is filled, the roller can be rotated, and the stacking frame is lifted or put down through the sling, so that the stacking frame and the cultivation tray are positioned on the same horizontal plane, the water chestnut seeds can be conveniently placed by workers, and the space utilization rate of the cultivation room is improved;
3. in the cultivation tray, the tray can be moved to the position below the water tank through the fifth conveying belt, and then the cultivation holes are moved to the front of workers, so that the cultivation tray is conveniently filled;
4. first water pump can extract the culture solution in the culture solution box to the cultivation dish of the superiors in, and the culture solution flows to the lower floor cultivation dish through the delivery port when the water level is higher than the delivery port in to make the culture solution constantly flow in each cultivation dish, and can guarantee the equilibrium of nutrient solution in the cultivation dish to culture solution incasement interpolation culture solution, the bud seedling is grown the vigor unanimously, also is convenient for get rid of the harmful substance that the growth of water chestnut discharged in the culture solution.
Drawings
Fig. 1 is a schematic structural diagram of an integrated system for aseptic cultivation of water chestnut tissue culture seeds.
FIG. 2 is a schematic view of the structure of the sterilization tank.
Fig. 3 is a schematic view of the installation structure of the spray device.
Fig. 4 is a schematic view of the installation structure of the second conveyor belt.
Fig. 5 is a schematic structural view of the stack frame.
FIG. 6 is a schematic view of the structure of the circulation system.
FIG. 7 is a schematic view of the structure of the cultivation plate.
Fig. 8 is a schematic view showing a structure when the tray is moved to below the cultivation plate.
In the figure: 1-a sterilizing pool, 2-a first conveyor belt, 3-a roller set, 4-a spraying device, 5-a first bracket, 6-a second conveyor belt, 7-a second bracket, 8-a cultivating tray, 9-a return pipe, 10-a liquid conveying pipe, 11-a culture solution box, 12-a first water pump, 101-a sterilizing cavity, 102-a cleaning cavity, 103-a water spraying pipe, 104-a second water pump, 41-a third water pump, 42-a spray head, 51-a support rod, 52-a first sling, 53-a first pulley, 54-a second sling, 55-a second pulley, 56-a roller, 61-a stacking frame, 62-a guide rail, 63-a sliding block, 64-a rack, 65-a gear, 81-a fifth conveyor belt and 82-an upper tray, 83-lower tray, 84-hanging lug, 85-water tank, 86-driving wheel, 87-water inlet, 88-water outlet, 89-water inlet pipe and 90-cultivation hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, in the embodiment of the present invention, an integrated system for aseptic cultivation of water chestnut species includes a sterilization tank 1, a first conveyor belt 2, a roller group 3, a second conveyor belt 6, a bracket 7 and cultivation trays 8, wherein the first conveyor belt 2 is installed in the sterilization tank 1, the roller group 3 is installed obliquely above the first conveyor belt 2, the roller group 3 is parallel to the first conveyor belt 2, the rear end of the roller group 3 is located below the front end of the first conveyor belt 2, a spraying device 4 is installed on the sterilization tank 1 corresponding to the roller group 3, a first bracket 5 is installed on the front side of the sterilization tank, the second conveyor belt 6 is installed in the first bracket 5, a second bracket 7 is installed on the front side of the first bracket 5, a plurality of cultivation trays 8 are installed in the second bracket 7, the water chestnut species is sterilized in the sterilization tank 1, the water chestnut species is conveyed to the roller group 3 by the second conveyor belt 2, and is cleaned by the spraying device 4, the cleaned water chestnut seeds move forward under the drive of the roller group 3 and finally fall onto the second conveyor belt 6, the water chestnuts are driven to the left side by the second conveyor belt 6, and then the water chestnut seeds are manually placed in the cultivation disc 8.
Referring to fig. 2 and 3, a sterilization chamber 102 is arranged at the rear side inside the sterilization tank 1, a disinfectant is filled in the sterilization chamber 102, a cleaning chamber 102 is arranged in the sterilization tank 1 corresponding to the front side of the sterilization chamber 101, purified water is filled in the cleaning chamber 102, a second water pump 104 is installed on the right wall of the sterilization tank 1, a water inlet of the second water pump 104 is connected with the right wall of the sterilization tank 1, a water outlet of the second water pump 104 is connected with a water spray pipe 103, the rear end of the water spray pipe 103 is connected with the rear wall of the sterilization tank 1, the spray device 4 comprises a spray head 42 and a third water pump 41, the third water pump 41 is installed on the sterilization tank 1, a water outlet pipe of the third water pump 41 is inserted in the cleaning chamber 103, the water outlet pipe of the third water pump 41 is connected with the spray head 42, the spray head 42 is installed on the front wall of the sterilization tank 1, the purified water in the cleaning chamber 103 is pumped out by the third.
Referring to fig. 4 and 5, a guide rail 62 is installed on the first support 5, a stacking frame 61 is installed above the guide rail 62, a plurality of sliding blocks 63 are installed at the bottom of the stacking frame 61, the sliding blocks 63 are erected on the guide rail 62, the second conveyor 6 is installed in the stacking frame 61, a rack 64 is installed at the bottom of the stacking frame 61, a motor is installed in the first support 5, a gear 65 is installed on a rotating shaft of the motor, the gear 65 is meshed with the rack 64, a plurality of support rods 51 are installed on the first support 5, the support rods 51 are slidably connected with the first support 5, the guide rail 62 is fixedly installed on the support rods 51, a first pulley 53 is installed on the right side of the top end of the first support 5, a second pulley 55 is installed on the left side of the top end of the first support 5, a second sling 54 and a first sling 52 are installed in the first support 5, the right ends of the second, the left side of the first support 5 is provided with a roller 56, the left end of a first sling 52 bypasses a first pulley 53 and a second pulley 55 to be connected with the roller 56, the left end of a second sling 54 bypasses the second pulley 55 to be connected with the roller 56, the left side of the water chestnut is driven by the second conveyor belt 6 to the left side, the water chestnut seeds are stacked on the left side of the stacking frame 61, then the water chestnut seeds are manually placed in the tray in the cultivating tray 8, the roller 56 can lift or put down the stacking frame 61 through the sling, the stacking frame 61 and the cultivating tray 8 are located on the same horizontal plane, and workers can conveniently place the water chestnut seeds.
Referring to fig. 6-8, a water outlet 88 is arranged on the front wall of the cultivation tray 8, a water inlet 87 is arranged on the left side of the water outlet 88, the cultivation trays 8 are installed in the second support 7 in a layered manner, a culture solution tank 11 is arranged on the front side of the second support 7, a first water pump 12 is installed on the culture solution tank 11, a water pumping pipe of the first water pump 12 is inserted into the culture solution tank 11, a liquid conveying pipe 10 is fixedly connected to a water outlet pipe of the first water pump 12, the top of the liquid conveying pipe 10 is connected with the water inlet 87 on the uppermost layer, a return pipe 9 is connected to the lower side of the water outlet 88, the bottom of the return pipe 9 on the lowermost layer is connected with the water inlet 87 on the lower side thereof, the bottom of the return pipe 9 on the lowermost layer is inserted into the culture solution tank 11, the first water pump 12 can pump the culture solution in the culture;
the tray 8 includes fifth conveyer belt 81, go up tray 82, lower tray 83, hangers 84, basin 85 and drive wheel 86, drive wheel 86 is all installed to basin 85's four corners department, the cover is equipped with fifth conveyer belt 81 on two drive wheel 86 on the fore-and-aft direction, be provided with tray 83 down in the basin 85, tray 83 top is provided with tray 82 down, it all is connected with fifth conveyer belt 81 through the lifting rope with lower tray 83 to go up tray 82, the left and right sides of basin 85 all is provided with a plurality of hangers 84, install a plurality of inlet tube 89 in the basin 85, inlet tube 89 and water inlet 87 intercommunication.
The working principle of the invention is as follows:
the invention relates to a water chestnut tissue culture and seed fruit aseptic culture integrated system, when in use, water chestnut seeds and fruits are placed in a sterilization tank 1, disinfectant is filled in the sterilization tank 1, after being soaked for 30 minutes, a third conveyor belt 101 and a second conveyor belt 2 are started to convey the water chestnut seeds and fruits to a roller shaft group 3, then a spraying device 4 is started to clean the water chestnut seeds and fruits through a spray head 42, the cleaned water chestnut seeds and fruits are driven by the roller shaft group 3 to move forwards and finally fall into a stacking frame 61, the stacking frame 61 is driven by the second conveyor belt 6 to the left side, so that the water chestnut seeds and fruits are stacked on the left side of the stacking frame 61, then the water chestnut seeds and fruits are placed into a tray in a culture tray 8 through manpower, wherein a roller 56 can lift or put down the stacking frame 61 through a sling, so that the stacking frame 61 and the culture tray 8 are positioned on the same horizontal plane, thus facilitating workers to place the water chestnut seeds and fruits, the cultivation tray 8 is internally provided with a driving wheel 86 and a fifth conveyor belt 81, the tray is installed on the fifth conveyor belt 81, the tray can be moved to the lower part of a water tank 85 through the fifth conveyor belt 81, when the tray is filled, row-by-row filling can be carried out, the fifth conveyor belt 81 is moved forward for a certain distance after one row is filled, after all cultivation trays 8 are filled, the first water pump 12 can be started, the first water pump 12 can pump the culture solution in the culture solution box 11 into the cultivation tray 8 on the uppermost layer, when the water level is higher than the water outlet 88, the culture solution flows into the cultivation tray 8 on the lower layer through the water outlet 88, and when the water is discharged, the second conveyor belt 6 enables the water chestnuts to be stacked to the right side of the stacking frame 61, the gear 65 drives the rack 64 to move to the right side, so that the stacking frame 61 moves to the right side, the right end of the stacking frame 61 extends out of a sterile room, and the.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims (8)

1. An integrated system for cultivating water chestnut in a tissue culture way and aseptically cultivating seeds comprises a sterilizing pool (1), a first conveyor belt (2), a roller group (3), a second conveyor belt (6), a bracket (7) and a cultivating tray (8), the first conveyor belt (2) is arranged in the sterilizing tank (1), the roller shaft group (3) is arranged obliquely above the first conveyor belt (2), the roller shaft group (3) is parallel to the first conveyor belt (2), the rear end of the roller shaft group (3) is positioned below the front end of the first conveyor belt (2), a spraying device (4) is arranged on the sterilizing tray (1) corresponding to the roller shaft group (3), the sterilizing tank is characterized in that a first support (5) is arranged on the front side of the sterilizing tank, a second conveyor belt (6) is installed in the first support (5), a second support (7) is arranged on the front side of the first support (5), and a plurality of cultivation trays (8) are installed in the second support (7).
2. The water chestnut tissue culture seed sterile cultivation integrated system according to claim 1, wherein a sterilization cavity (101) is arranged on the rear side inside the sterilization tank (1), a cleaning cavity (102) is arranged in the sterilization tank (1) corresponding to the front side of the sterilization cavity (101), a second water pump (104) is installed on the right wall of the sterilization tank (1), a water inlet of the second water pump (104) is connected with the right wall of the sterilization tank (1), a water outlet of the second water pump (104) is connected with a water spray pipe (103), and the rear end of the water spray pipe (103) is connected with the rear wall of the sterilization tank (1).
3. A water chestnut tissue culture seed sterile cultivation integrated system according to claim 1, characterized in that the spraying device (4) comprises a spray head (42) and a third water pump (41), the third water pump (41) is installed on the sterilization tank (1), a water pumping pipe of the third water pump (41) is inserted into the cleaning cavity (103), a water outlet pipe of the third water pump (41) is connected with the spray head (42), and the spray head (42) is installed on the front wall of the sterilization tank (1).
4. The water chestnut tissue culture seed sterile cultivation integrated system according to claim 1, wherein a guide rail (62) is installed on the first support (5), a stacking frame (61) is arranged above the guide rail (62), a plurality of sliding blocks (63) are installed at the bottom of the stacking frame (61), the sliding blocks (63) are erected on the guide rail (62), the second conveyor belt (6) is installed in the stacking frame (61), a rack (64) is installed at the bottom of the stacking frame (61), a motor is installed in the first support (5), a gear (65) is installed on a rotating shaft of the motor, and the gear (65) is meshed with the rack (64).
5. The water chestnut tissue culture seed sterile cultivation integrated system according to claim 4, wherein a plurality of support rods (51) are installed on the first support (5), the support rods (51) are slidably connected with the first support (5), the guide rail (62) is fixedly installed on the support rods (51), a first pulley (53) is installed on the right side of the top end of the first support (5), a second pulley (55) is installed on the left side of the top end of the first support (5), a second sling (54) and a first sling (52) are arranged in the first support (5), the right ends of the second sling (54) and the first sling (52) are respectively fixedly connected with the left support rod and the right support rod (51), a roller (56) is arranged on the left side of the first support (5), and the left end of the first sling (52) is connected with the roller (56) by bypassing the first pulley (53) and the second pulley (55), the left end of the second sling (54) is connected with the roller (56) by passing through a second pulley (55).
6. The integrated system for sterile cultivation of water chestnut tissue culture seed according to claim 1, a water outlet (88) is arranged on the front wall of the cultivation tray (8), a water inlet (87) is arranged on the left side of the water outlet (88), the cultivation trays (8) are installed in the second bracket (7) in a layered manner, a culture solution box (11) is arranged on the front side of the second support (7), a first water pump (12) is installed on the culture solution box (11), a water suction pipe of the first water pump (12) is inserted into the culture solution box (11), a liquid conveying pipe (10) is fixedly connected to a water outlet pipe of the first water pump (12), the top of the liquid conveying pipe (10) is connected with a water inlet (87) on the uppermost layer, a return pipe (9) is connected below the water outlet (88), the bottom of the return pipe (9) is connected with a water inlet (87) below the return pipe, and the bottom of the return pipe (9) at the lowest layer is inserted in the culture solution box (11).
7. The integrated system for sterile cultivation of water chestnut tissue culture seed according to claim 1, the breeding tray (8) comprises a fifth conveyor belt (81), an upper tray (82), a lower tray (83), a hanging lug (84), a water tank (85) and a driving wheel (86), the four corners of the water tank (85) are provided with driving wheels (86), the two driving wheels (86) in the front and back direction are sleeved with a fifth conveyor belt (81), a lower tray (83) is arranged in the water tank (85), an upper tray (82) is arranged above the lower tray (83), the upper tray (82) and the lower tray (83) are connected with a fifth conveyor belt (81) through lifting ropes, the left side and the right side of the water tank (85) are provided with a plurality of hangers (84), and the upper tray (82) and the lower tray (83) are internally provided with a plurality of cultivation holes (90).
8. The integrated system for the aseptic cultivation of water-chestnut tissue culture seeds and fruit as claimed in claim 7, wherein a plurality of water inlet pipes (89) are installed in the water tank (85), and the water inlet pipes (89) are communicated with the water inlet (87).
CN202010804435.7A 2020-08-12 2020-08-12 Water chestnut tissue culture and seed fruit sterile cultivation integrated system Pending CN111837962A (en)

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CN206196587U (en) * 2016-09-30 2017-05-31 华星环球(深圳)农业有限公司 Cage lifting device is used in houseplant growth
CN206575940U (en) * 2017-03-03 2017-10-24 德州市农业科学研究院 A kind of three-dimensional adjustable culture rack device
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CN207167177U (en) * 2017-09-27 2018-04-03 福建农林大学 A kind of plant cultivation frame for plant factor
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CN109006325A (en) * 2018-08-14 2018-12-18 王军 A kind of method for culturing seedlings of seedling wood flower
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CN209047001U (en) * 2018-10-24 2019-07-02 富源县金地魔芋种业有限公司 A kind of konjac seed chlorination equipment
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Publication number Priority date Publication date Assignee Title
CN111919754A (en) * 2020-09-16 2020-11-13 王灵琳 Potato tissue culture method
CN111919754B (en) * 2020-09-16 2021-06-01 乐陵希森马铃薯产业集团有限公司 Potato tissue culture method

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