CN111830697A

CN111830697A - Microscope for stem cell detection and observation

Info

Publication number: CN111830697A
Application number: CN202010631964.1A
Authority: CN
Inventors: 吴晶晶
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-03
Filing date: 2020-07-03
Publication date: 2020-10-27

Abstract

The invention discloses a microscope for stem cell detection and observation, relates to the technical field of cell microscopes, and aims to solve the problems that most droppers in the prior art need to be held by hands for use, the dropper is difficult to hold by hands when a microscope is used for observation, and the dropper is not easy to master when a reagent is dripped. The bottom of cell monitoring microscope is provided with fixed baseplate, one side of fixed baseplate is provided with lighting switch, fixed baseplate's top is provided with the elevating sill bar, the top of elevating sill bar is provided with concave type lifter, one side of concave type lifter is provided with bears branch, the top of bearing branch is provided with the converter, the one end of converter is provided with the observation eyepiece, the both sides of converter are provided with focusing knob, and focusing knob has two, the below of converter is provided with cell objective, the below of bearing branch is provided with the objective table, the surface of objective table is provided with the lift groove.

Description

Microscope for stem cell detection and observation

Technical Field

The invention relates to the technical field of cell microscopes, in particular to a microscope for detecting and observing stem cells.

Background

The function of the stem cell population is to control and maintain cell regeneration, and in general, there exists an intermediate progenitor cell population, called "committed progenitor cells", between the stem cells and their terminally differentiated progeny, which have limited expansion capacity and limited differentiation potential. The function of these cell populations is to increase the number of differentiated cells produced after each division of the stem cells, which have the ability to self-renew, but the division of the stem cells is in fact relatively asymmetric, and although the structural meaning of the stem cells on the morphological and molecular biological levels can help to define stem cells, the definition of stem cells must be based on the functional basis, functionally, stem cells are cells with a multipotent, self-renewing capacity, being the most primitive cells at the top of the cell line origin, in adult organs, stem cells can repair tissues by constantly dividing, or are in a quiescent state as in mammalian brain tissue. Stem cells are capable of self-renewal during their development by symmetrically dividing to expand their numbers, or self-renewal by asymmetrically dividing and generating more progenitor cells of different differentiation types, capable of differentiating in vivo to produce cells of a certain tissue type, and capable of differentiating under specific circumstances that require the use of a reagent dropper.

However, most of the existing droppers need to be held by hands for use, and when a microscope is used for observation, the dropper is difficult to hold by hands, and meanwhile, the dropper is not easy to master when a reagent is dripped; therefore, the existing requirements are not satisfied, and a microscope for stem cell detection and observation is provided for the requirement.

Disclosure of Invention

The present invention is directed to a microscope for stem cell examination and observation, which solves the problems that many of the pipettes proposed in the background art described above need to be held by hand, and that it is laborious to hold the pipette by hand when observing using a microscope, and that it is not easy to grasp when dropping a reagent.

In order to achieve the purpose, the invention provides the following technical scheme: a microscope for stem cell detection and observation comprises a cell monitoring microscope, wherein a fixed base is arranged at the bottom of the cell monitoring microscope, a lighting switch is arranged on one side of the fixed base, an elevating bottom rod is arranged above the fixed base, a concave lifting rod is arranged above the elevating bottom rod, a bearing support rod is arranged on one side of the concave lifting rod, a converter is arranged above the bearing support rod, one end of the converter is provided with an observation eyepiece, two focusing knobs are arranged on two sides of the converter, a cell objective lens is arranged below the converter, an object stage is arranged below the bearing support rod, a lifting groove is arranged on the outer surface of the object stage, a culture dish placing plate is arranged above the object stage, and a dropper clamping assembly is arranged on one side of the culture dish placing plate, an adjusting lamp arm is arranged on one side of the dropper clamping assembly, an illuminating lamp is arranged at the other end of the adjusting lamp arm, an object end adjusting knob is arranged below the object stage, and a threaded screw rod is arranged inside the object end adjusting knob.

Preferably, the burette clamping assembly comprises a supporting sleeve, an extending support rod is arranged above the supporting sleeve, a burette clamping ring is arranged above the extending support rod, a clamping screw rod is arranged above the extending support rod, and a rubber pad is arranged at the bottom of the clamping screw rod.

Preferably, the support sleeve is in telescopic connection with the extension support rod, and a sponge layer is arranged on the inner side of the dropper clamping ring.

Preferably, the extension supporting rod is rotatably connected with the dropper clamping ring through a rotating shaft, the extension supporting rod is rotatably connected with the clamping screw rod through an internal thread, and the clamping screw rod is connected with the rubber pad through adhesive.

Preferably, the outside of concave type lifter is provided with mirror end adjust knob, one side of the inside of concave type lifter is provided with the main drive axle, and the main drive axle has two, the opposite side of the inside of concave type lifter is provided with vice drive axle, and vice drive axle has two, the surface of main drive axle and vice drive axle all is provided with the tooth's socket drive belt.

Preferably, the mirror end adjusting knob is connected with the main driving shaft through a support rod, the main driving shaft and the auxiliary driving shaft are rotatably connected with the concave lifting rod, and the main driving shaft and the auxiliary driving shaft are connected with the tooth groove transmission belt in a combined mode.

Preferably, the illuminating lamp is connected with the objective table through an adjusting lamp arm, the elevating bottom rod is connected with the concave lifting rod through a screw, and the bearing support rod is connected with the concave lifting rod in a sliding mode.

Preferably, the bearing support rod is connected with the converter through a clamping groove, the converter is fixedly connected with the observation eyepiece and the cell objective, and the converter is rotatably connected with the focusing knob.

Preferably, the bearing support rod is connected with the objective table through a bolt, the object end adjusting knob is rotatably connected with the objective table, the threaded screw rod is connected with the culture dish placing plate in a combined mode, and the object end adjusting knob is rotatably connected with the threaded screw rod through an internal thread.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, a culture dish containing stem cells is placed in a culture dish placing plate above an objective table, after the height is adjusted, reagents required by stem cell differentiation are sucked through a dropper, then the dropper containing the reagents passes through a dropper snap ring on one side of the placing plate, after the dropper enters the snap ring, an upper clamping screw rod is screwed down, so that the dropper is fixed, a rubber pad is arranged at the contact part of the screw rod and the dropper, so that the dropper can be prevented from being worn during clamping, and meanwhile, the bottom of the snap ring is connected with an extension support rod through a rotating shaft, so that during observation, the dropper is fixed on one side of the culture dish without being held by hands, and is rotated downwards during use, and the reagents are dripped into the culture dish;

2. the invention uses one end of the bearing support rod to install the ocular lens and the objective lens needed for monitoring, the other end is installed in the tooth socket transmission belt on the two sides of the concave lifting rod, the two tooth socket transmission belts are respectively provided with a main driving shaft and an auxiliary driving shaft, each driving shaft is provided with two driving shafts, one main driving shaft is connected with the mirror end adjusting knob on the outer side of the concave lifting rod, when in use, the main driving shaft can be driven to rotate by rotating the mirror end adjusting knob, when the main driving shaft rotates, the crawler belt on the surface of the main driving shaft also rotates, at this time, the bearing support rod can move upwards or downwards to match the height adjustment of the object end at the bottom, thereby finding the optimal observation distance.

Drawings

FIG. 1 is an overall front view of the present invention;

FIG. 2 is a schematic view of the object-side adjusting knob according to the present invention;

FIG. 3 is a schematic diagram of a burette clamping assembly of the present invention;

FIG. 4 is a schematic view of a concave lifting rod according to the present invention;

fig. 5 is a top view of the stage of the present invention.

In the figure: 1. a cell monitoring microscope; 2. a fixed base; 3. a light switch; 4. raising the bottom rod; 5. a concave lifting rod; 6. an object end adjusting knob; 7. an object stage; 8. a mirror end adjusting knob; 9. a load bearing strut; 10. an observation eyepiece; 11. a culture dish placing plate; 12. a cell objective lens; 13. a focusing knob; 14. an illuminating lamp; 15. adjusting the lamp arm; 16. a dropper gripping assembly; 17. a support sleeve; 18. an extension strut; 19. a dropper snap ring; 20. clamping the screw rod; 21. a rubber pad; 22. a threaded lead screw; 23. a converter; 24. a tooth socket transmission belt; 25. a main drive shaft; 26. a secondary drive shaft; 27. a lifting groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to fig. 1-5, an embodiment of the present invention is shown: a microscope for detecting and observing stem cells comprises a cell monitoring microscope 1, wherein a fixed base 2 is arranged at the bottom of the cell monitoring microscope 1, an illumination switch 3 is arranged on one side of the fixed base 2 and used for controlling the opening and closing of an illumination lamp, an elevating bottom rod 4 is arranged above the fixed base 2, a concave lifting rod 5 is arranged above the elevating bottom rod 4, a bearing support rod 9 is arranged on one side of the concave lifting rod 5, a converter 23 is arranged above the bearing support rod 9, an observation ocular lens 10 is arranged at one end of the converter 23, focusing knobs 13 are arranged on two sides of the converter 23, the focusing knobs 13 are respectively used for fine focus adjustment and coarse focus adjustment, a cell objective lens 12 is arranged below the converter 23, an object stage 7 is arranged below the bearing support rod 9, a lifting groove 27 is arranged on the outer surface of the object stage 7, a culture dish placing plate 11 is arranged above the object stage 7, the cell placing device is used for placing a culture dish filled with cells, a dropper clamping assembly 16 is arranged on one side of a culture dish placing plate 11, an adjusting lamp arm 15 is arranged on one side of the dropper clamping assembly 16, an adjusting illuminating lamp is arranged, an illuminating lamp 14 is arranged at the other end of the adjusting lamp arm 15 and provides light illumination, an object end adjusting knob 6 is arranged below an object stage 7, a threaded screw rod 22 is arranged inside the object end adjusting knob 6, and the height of the placing plate is controlled.

Further, the burette clamping assembly 16 comprises a supporting sleeve 17, an extending support rod 18 is arranged above the supporting sleeve 17, so that the height of the burette clamping assembly can be conveniently adjusted, a burette clamping ring 19 is arranged above the extending support rod 18, the burette is fixed, a clamping screw rod 20 is arranged above the extending support rod 18, and a rubber pad 21 is arranged at the bottom of the clamping screw rod 20, so that the burette is prevented from being worn when being clamped.

Further, a supporting sleeve 17 is connected with the objective table 7 through a clamping groove, the supporting sleeve 17 is in telescopic connection with an extension supporting rod 18, height adjustment is achieved, and a sponge layer is arranged on the inner side of the dropper clamping ring 19 to protect the dropper.

Furthermore, the extension support rod 18 is rotatably connected with the dropper snap ring 19 through a rotating shaft to control the horizontal angle of the dropper, the extension support rod 18 is rotatably connected with the clamping screw rod 20 through internal threads to realize the clamping operation of the dropper, and the clamping screw rod 20 is connected with the rubber pad 21 through adhesive.

Further, the outside of concave type lifter 5 is provided with mirror end adjust knob 8, one side of the inside of concave type lifter 5 is provided with main drive axle 25, and main drive axle 25 has two, can rotate through manual initiative, the opposite side of the inside of concave type lifter 5 is provided with auxiliary drive axle 26, and auxiliary drive axle 26 has two, rotate passively, main drive axle 25 and auxiliary drive axle 26's surface all is provided with tooth's socket drive belt 24, be used for being connected fixedly with bearing branch 9, thereby control bearing branch 9 reciprocates.

Further, mirror end adjust knob 8 passes through branch with main drive shaft 25 and is connected, can drive a main drive shaft 25 through rotating mirror end adjust knob 8 and rotate, and main drive shaft 25 and vice drive shaft 26 rotate with concave type lifter 5 and be connected, realize bearing support 9's regulation of reciprocating, main drive shaft 25 and vice drive shaft 26 and tooth's socket drive belt 24 built-up connection.

Further, light 14 is connected through adjusting lamp arm 15 with objective table 7, and elevating bottom bar 4 passes through bolted connection with concave type lifter 5, reinforcing stability, bears branch 9 and concave type lifter 5 sliding connection.

Furthermore, the bearing support rod 9 is connected with the converter 23 through a clamping groove, the converter 23 is fixedly connected with the observation eyepiece 10 and the cell objective lens 12, and the converter 23 is rotatably connected with the focusing knob 13, so that the use is convenient.

Further, bearing support rod 9 passes through bolted connection with objective table 7, and thing end adjust knob 6 rotates with objective table 7 to be connected, and board 11 built-up connection is placed with the culture dish to screw lead screw 22, and thing end adjust knob 6 rotates through the internal thread with screw lead screw 22 to be connected, can upwards rise the lead screw through rotating thing end adjust knob 6.

The working principle is as follows: when the device is used, a culture dish filled with stem cells is placed in a culture dish placing plate 11 above an object stage 7, the bottom of the culture dish placing plate 11 is connected with a threaded screw rod 22, the height of the placing plate can be controlled to rise and fall by rotating an object end adjusting knob 6 arranged at the bottom of the object stage 7, because the object end adjusting knob 6 is fixedly connected with the object stage 7, the threaded screw rod 22 moves upwards or downwards when the knob rotates, meanwhile, an ocular lens and an objective lens required for monitoring are arranged at one end of a bearing support rod 9 at the upper part, the other end of the bearing support rod is arranged in tooth socket transmission belts 24 at two sides in a concave lifting rod 5, a main driving shaft 25 and an auxiliary driving shaft 26 are respectively arranged in the two tooth socket transmission belts, each driving shaft is provided with two driving shafts, one main driving shaft 25 is connected with a mirror end adjusting knob 8 at the outer side of the concave lifting rod 5, and when the device is used, the main driving shaft, when the main driving shaft 25 rotates, the caterpillar track on the surface of the main driving shaft also rotates, at this time, the bearing support rod moves upwards or downwards to match with the height adjustment of the object end at the bottom so as to find the optimal observation distance, after the adjustment is completed, the observation eyepiece 10 at one side of the converter 23 is matched with the cell objective 12 right above the culture dish to observe the stem cell tissue in the culture dish, in the observation process, the focal lengths of the eyepiece and the objective can be adjusted through the fine focus and the coarse focus knobs at two sides of the converter 23 so as to find the optimal observation distance of the stem cell, finally, the reagent required by stem cell differentiation is sucked through the dropper, the dropper filled with the reagent is penetrated through the dropper clamping ring 19 at one side of the placing plate, after the dropper enters the clamping ring, the clamping screw rod 20 above is screwed down so as to complete the fixation of the dropper, the rubber pad 21 is arranged at the contact part of the dropper and, can avoid causing wearing and tearing to the burette when pressing from both sides tightly, the bottom of snap ring links to each other with extension branch 18 through the pivot simultaneously, and when surveing like this, fix in one side of culture dish during the burette, need not to hand, rotates the burette downwards during the use, with reagent instil into in the culture dish.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A microscope for stem cell detection observation, comprising a cell monitoring microscope (1), characterized in that: the cell monitoring microscope is characterized in that a fixed base (2) is arranged at the bottom of the cell monitoring microscope (1), a lighting switch (3) is arranged on one side of the fixed base (2), an elevating bottom rod (4) is arranged above the fixed base (2), a concave lifting rod (5) is arranged above the elevating bottom rod (4), a bearing support rod (9) is arranged on one side of the concave lifting rod (5), a converter (23) is arranged above the bearing support rod (9), an observation eyepiece (10) is arranged at one end of the converter (23), focusing knobs (13) are arranged on two sides of the converter (23), two focusing knobs (13) are arranged, a cell objective lens (12) is arranged below the converter (23), an objective table (7) is arranged below the bearing support rod (9), and a lifting groove (27) is arranged on the outer surface of the objective table (7), the novel multifunctional incubator is characterized in that a culture dish placing plate (11) is arranged above the objective table (7), a burette clamping assembly (16) is arranged on one side of the culture dish placing plate (11), an adjusting lamp arm (15) is arranged on one side of the burette clamping assembly (16), an illuminating lamp (14) is arranged at the other end of the adjusting lamp arm (15), an object end adjusting knob (6) is arranged below the objective table (7), and a threaded screw rod (22) is arranged inside the object end adjusting knob (6).

2. The microscope according to claim 1, wherein: the burette clamping assembly (16) comprises a supporting sleeve (17), an extending support rod (18) is arranged above the supporting sleeve (17), a burette clamping ring (19) is arranged above the extending support rod (18), a clamping screw rod (20) is arranged above the extending support rod (18), and a rubber pad (21) is arranged at the bottom of the clamping screw rod (20).

3. The microscope according to claim 2, wherein: the support sleeve (17) is in telescopic connection with the extension support rod (18), and a sponge layer is arranged on the inner side of the dropper clamping ring (19).

4. The microscope according to claim 2, wherein: the extension support rod (18) is rotationally connected with the dropper clamping ring (19) through a rotating shaft, the extension support rod (18) is rotationally connected with the clamping screw rod (20) through an internal thread, and the clamping screw rod (20) is connected with the rubber pad (21) through adhesive.

5. The microscope according to claim 1, wherein: the utility model discloses a glasses, including concave type lifter (5), concave type lifter (5) outside is provided with mirror end adjust knob (8), one side of the inside of concave type lifter (5) is provided with main drive axle (25), and main drive axle (25) have two, the opposite side of the inside of concave type lifter (5) is provided with auxiliary drive axle (26), and auxiliary drive axle (26) have two, the surface of main drive axle (25) and auxiliary drive axle (26) all is provided with tooth's socket drive belt (24).

6. The microscope according to claim 5, wherein: the mirror end adjusting knob (8) is connected with a main driving shaft (25) through a support rod, the main driving shaft (25) and an auxiliary driving shaft (26) are rotatably connected with the concave lifting rod (5), and the main driving shaft (25) and the auxiliary driving shaft (26) are connected with a tooth groove transmission belt (24) in a combined mode.

7. The microscope according to claim 1, wherein: the illuminating lamp (14) is connected with the objective table (7) through an adjusting lamp arm (15), the elevating bottom rod (4) is connected with the concave lifting rod (5) through a screw, and the bearing support rod (9) is connected with the concave lifting rod (5) in a sliding mode.

8. The microscope according to claim 1, wherein: bear branch (9) and pass through the draw-in groove with converter (23) and be connected, converter (23) and observation eyepiece (10) and cell objective (12) fixed connection, converter (23) rotate with focusing knob (13) and be connected.

9. The microscope according to claim 1, wherein: bear branch (9) and pass through bolted connection with objective table (7), thing end adjust knob (6) are rotated with objective table (7) and are connected, board (11) built-up connection is placed with the culture dish in screw lead screw (22), thing end adjust knob (6) are connected through internal thread rotation with screw lead screw (22).