CN111826262B - Biological 3D printing system and method - Google Patents
Biological 3D printing system and method Download PDFInfo
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- CN111826262B CN111826262B CN201910313928.8A CN201910313928A CN111826262B CN 111826262 B CN111826262 B CN 111826262B CN 201910313928 A CN201910313928 A CN 201910313928A CN 111826262 B CN111826262 B CN 111826262B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y99/00—Subject matter not provided for in other groups of this subclass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/25—Process efficiency
Abstract
The invention discloses a biological 3D printing system and a biological 3D printing method, wherein the system comprises a medical imaging module, a sound field inversion module, an artificial intelligent module, an acoustic control module and a water tank, wherein the medical imaging module is used for imaging human tissues to obtain tissue slice images; the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating an ultrasonic pulse sequence corresponding to the synthesized target sound field; the artificial intelligent module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence; the sound control module is used for transmitting a target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspension cells in the water tank by utilizing the sound field, and constructing a biological tissue structure corresponding to human tissue. The invention can effectively ensure the bioactivity of cells and avoid damaging the cells during the cross-linking molding.
Description
Technical Field
The invention relates to the technical field of cell printing, in particular to a biological 3D printing system and method.
Background
Biological 3D printing is a technology capable of positioning and assembling biological materials or cell units according to an additive manufacturing principle under the drive of a digital three-dimensional model and manufacturing products such as tissue engineering scaffolds, tissue organs and the like, and can effectively solve the problem that the current transplanted organ sources are limited.
In the aspect of the current biological 3D printing technology, the biggest difficulty is reflected in cell activity and crosslinking formation. In the aspect of cell activity, for the printing mode of a liquid drop jet type and an extrusion forming type, the common characteristics of the two are that the two are provided with nozzles, and the cell activity of the printing mode with the nozzles is always a difficult problem, because the factors which cause the greatest damage to cells during cell printing are shear force caused by liquid flow during printing, and the damage to cells can be greatly increased by a nozzle structure when the caliber of the nozzle is smaller, in the printing mode with the nozzle structure, the cell activity and the printing precision are difficult to be obtained, the printing resolution is reduced by increasing the caliber of the nozzle, and the cell activity is reduced by reducing the caliber of the nozzle. Secondly, in the aspect of crosslinking, the biological ink pattern obtained by printing is fixed and molded through the modes of temperature control, chemical treatment, ultraviolet irradiation and the like, however, the crosslinking modes can possibly damage cells.
Disclosure of Invention
The application provides a biological 3D printing system and method, which can solve the technical problems that the biological 3D printing technology in the prior art is difficult to ensure the activity of cells and the cells are easy to damage during crosslinking and forming.
Specifically, the first aspect of the invention provides a biological 3D printing system, which comprises a medical imaging module, a sound field inversion module, an artificial intelligence module, an acoustic control module and a water tank;
the medical imaging module is used for imaging human tissues to obtain tissue slice images;
the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
the artificial intelligent module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
the sound control module is used for transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspension cells in the water tank by utilizing the sound field, and constructing a biological tissue structure corresponding to the human tissue.
Preferably, the sound field inversion module is specifically configured to:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form a tissue pattern;
and setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation.
Preferably, the acoustic steering module comprises an array ultrasonic transducer, a signal generator and a power amplifier;
the array ultrasonic transducer is an ultrasonic transducer based on a surface array or an ultrasonic transducer based on a ring array, the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal.
Preferably, the acoustic steering module further comprises a beam combiner;
each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel in the beam synthesizer, the signal generator and the power amplifier.
Preferably, the array ultrasound transducer is located in the water tank.
Preferably, the water tank contains bio-ink containing suspended cells and growth factors.
Preferably, the biological 3D printing system further comprises a flow control module for uniformly distributing suspended cells within the water tank in a silent field space.
Preferably, the flow control module comprises a water pump and a control circuit, wherein the water pump is positioned at the bottom of the water tank, and the control circuit controls the water pump to control suspended cells in the water tank to be uniformly distributed in a silent field space.
A second aspect of the invention provides a biological 3D printing method comprising:
imaging human tissue to obtain a tissue slice image;
taking the obtained tissue slice image as a target sound field, and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
and transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspension cells in the water tank by utilizing the sound field, and constructing a biological tissue structure corresponding to the human tissue.
Preferably, the calculating and synthesizing the ultrasonic pulse sequence corresponding to the target sound field includes:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form a tissue pattern;
and setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation.
Compared with the prior art, the biological 3D printing system provided by the invention does not comprise a nozzle in the traditional method, but adopts the acoustic control technology, and the acoustic control technology has the advantages of non-contact and nondestructive, so that the biological activity of cells can be effectively ensured, and the cells can be controlled in parallel, so that the printing based on the surface or the three-dimensional can be realized except the traditional printing based on the point, and the printing speed is higher; meanwhile, because the sound field can generate stress and deformation on cells in the sound field, the invention can also provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and ensure that the three-dimensional complex structure assembled by discrete cells is fixedly formed, thereby avoiding the damage problem to the cells during cross-linking forming.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are necessary for the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention and that other drawings may be obtained from them without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a biological 3D printing system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of another embodiment of a biological 3D printing system;
FIG. 3 is a schematic flow chart of steps of a biological 3D printing method according to an embodiment of the present invention;
fig. 4 is a schematic flow chart of the substeps of the biological 3D printing method according to the embodiment of the invention.
Detailed Description
In order to make the objects, features and advantages of the present invention more comprehensible, the technical solutions in the embodiments of the present invention will be clearly described in conjunction with the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a biological 3D printing system according to an embodiment of the present invention, and in an embodiment of the present invention, a biological 3D printing system 100 includes a medical imaging module 101, a sound field inversion module 102, an artificial intelligence module 103, an acoustic control module 104, and a water tank 105.
The medical imaging module 101 is configured to image human tissue to obtain tissue slice images, i.e. biological tissue structure big data, where the imaging mode may be MRI (Magnetic Resonance Imaging ) or ultrasound imaging.
The sound field inversion module 102 is configured to take the obtained tissue slice image as a target sound field, and calculate and synthesize an ultrasonic pulse sequence corresponding to the target sound field. The ultrasonic pulse sequence is an ultrasonic pulse sequence emitted by an array ultrasonic transducer of the target sound field.
The artificial intelligence module 103 is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence.
The deep neural network learning model can adopt a conventional deep neural network (Deep Neural Networks, DNN) model, and the neural network layers in the DNN model can be divided into three types, namely an input layer, a hidden layer and an output layer, wherein generally, the first layer is the input layer, the last layer is the output layer, and the middle layers are all hidden layers.
The acoustic control module 104 is configured to emit a target ultrasonic pulse sequence, establish a sound field corresponding to the digital model of the target three-dimensional structure, and capture suspended cells in the water tank 105 by using the sound field to construct a biological tissue structure corresponding to the human tissue.
Specifically, the suspended cells are captured by utilizing the acoustic force effect generated by the sound field established in the space, the biological tissue structure corresponding to the accurate three-dimensional structure digital model is built layer by layer or integrally in three dimensions, and then the three-dimensional structure assembled by acoustic control of the dispersed suspended cells is fixed and formed in a natural growth mode.
The water tank 105 is a 3D printing area, the water tank 105 is filled with bio-ink containing suspended cells and growth factors, and the suspended cells can be synthesized and assembled in the water tank 105 to form a specific biological tissue structure by utilizing a sound field. Specifically, the water tank 105 is made of biocompatible medical stainless steel, quartz glass, or the like.
Specifically, the acoustic field inversion module 102 is specifically configured to:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form a tissue pattern; and setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation.
The acoustic wave equation is derived from a continuous equation and a momentum equation, and describes an important partial differential equation of the propagation of acoustic waves in a medium.
Compared with the prior art, the biological 3D printing system 100 provided by the embodiment of the invention does not have nozzles in the traditional method, but adopts the acoustic control technology, and the acoustic control technology has the advantages of non-contact and nondestructive, so that the biological activity of cells can be effectively ensured, and the cells can be controlled in parallel, so that the printing based on the surface or the three-dimensional can be realized besides the traditional printing based on the points, and the printing speed is higher; meanwhile, because the sound field can generate stress and deformation on cells in the sound field, the invention can also provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and ensure that the three-dimensional complex structure assembled by discrete cells is fixedly formed, thereby avoiding the damage problem to the cells during cross-linking forming.
Further, in the embodiment of the present invention, the acoustic steering module 104 includes an array ultrasonic transducer, a signal generator, and a power amplifier. The array ultrasonic transducer can be an ultrasonic transducer based on a surface array or an ultrasonic transducer based on a ring array; the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal. In addition, the transmit signal of the signal generator may be a continuous sinusoidal signal, or may be a pulsed sinusoidal signal.
Among them, the function of the array ultrasonic transducer is to convert the input electric power into mechanical power (i.e., ultrasonic waves) and transmit the mechanical power out, while consuming a small part of the power itself.
In order to better understand the invention, the embodiment of the invention provides an array ultrasonic transducer, which can be composed of a shell, a matching layer, a piezoelectric ceramic area array transducer, a back lining and an outgoing cable. The piezoelectric ceramic area array transducer is made of PZT-5 piezoelectric material polarized in the thickness direction. The piezoelectric ceramic area array transducer has 4 blocks, and each area array has 4096 array elements. Piezoelectric ceramic area transducers are used as basic ultrasonic transducers, from which ultrasonic signals are transmitted or received. The received ultrasonic signals can carry out ultrasonic imaging on the printed organ, and the anatomical structure and growth condition of the organ can be observed in real time. The imaging mode can be to obtain a two-dimensional B mode image based on a traditional pulse echo mode, or to obtain an ultrasonic CT two-dimensional image through a transmission mode, and to reconstruct a three-dimensional image through the two-dimensional image or directly obtain the three-dimensional image through three-dimensional volume data.
Further, the acoustic steering module 104 also includes a beam combiner. Wherein each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel of the beam synthesizer, the signal generator and the power amplifier. The beam synthesizer is used for controlling the amplitude and the phase of the pulse signals emitted by the array ultrasonic transducer, and the control mode can be realized by software or hardware.
Wherein the above-mentioned array ultrasonic transducer is located in the water tank 105 so that the suspended cells in the water tank 105 can be captured by using a sound field.
The biological 3D printing system 100 provided by the embodiment of the invention, the acoustic control module 104 includes an array ultrasonic transducer, a signal generator and a power amplifier, wherein the array ultrasonic transducer can be an ultrasonic transducer based on a planar array or an ultrasonic transducer based on a ring array, so that cells can be assembled into a two-dimensional or three-dimensional complex structure by using the acoustic control module 104.
Further, based on the above embodiment, referring to fig. 2, fig. 2 is a schematic diagram of another structure of a biological 3D printing system according to an embodiment of the invention, and in the embodiment of the invention, the biological 3D printing system 100 includes a medical imaging module 101, a sound field inversion module 102, an artificial intelligence module 103, an acoustic control module 104, a water tank 105 and a flow control module 106.
Wherein the flow control module 106 is configured to uniformly distribute suspended cells in the water tank 105 in the silence field space.
It can be understood that when the floating cells in the water tank 105 are captured by using the sound field, if the floating cells in the water tank 105 are unevenly distributed, the cells cannot be captured in the area where the cells are originally captured, so that the capturing result is inaccurate, and the requirement of 3D printing cannot be met. Therefore, in the embodiment of the present invention, in order to improve the accuracy of 3D printing, the flow control module 106 is used to uniformly distribute the suspended cells in the water tank 105 in the silent field space.
Specifically, the flow control module 106 includes a water pump and a control circuit, wherein the water pump is located at the bottom of the water tank 105, and the control circuit controls the water pump to control the suspended cells in the water tank 105 to be uniformly distributed in the silent field space. When the number of the water pumps is at least one, the control circuit simultaneously controls each water pump to form linkage among the water pumps.
The biological 3D printing system 100 provided by the embodiment of the invention further comprises a flow control module 106, and the accuracy of 3D printing can be effectively improved by maintaining the uniform distribution of the suspended cells in the water tank 105 in the silent field space.
The biological 3D printing system 100 provided in this embodiment has the following main advantages:
(1) The nozzle in the traditional method is not adopted, but the acoustic control technology is adopted, and the acoustic control technology has the advantages of non-contact and no damage, so that the damage to cells is avoided, and the bioactivity of the cells can be effectively ensured.
(2) Because the sound field pattern can be flexibly regulated and controlled through the ultrasonic pulse sequence, the invention can print more complex biological tissue structures.
(3) The parallel control can be performed on the cells, and besides the traditional dot-based printing, the plane-based or volume-based printing can be realized, so that the printing speed is higher.
(4) Because the sound field can generate stress and deformation to cells in the sound field, the invention can also provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and lead the three-dimensional complex structure assembled by discrete cells to be fixedly formed, thereby avoiding the damage problem to the cells during the cross-linking forming.
(5) The artificial intelligent module is utilized to learn biological structure big data (tissue slice images) and acoustic manipulation big data (ultrasonic pulse sequences), so that the spatial resolution of the tissue slice images in three dimensions of in-plane and interlayer can be improved, the synthesis precision of a target sound field is improved, and finally, a precise three-dimensional digital structure model and a corresponding ultrasonic emission pulse sequence are established.
(6) The cells can be assembled into a two-dimensional or three-dimensional complex structure using the acoustic manipulation module.
Further, an embodiment of the present invention further provides a biological 3D printing method, referring to fig. 3, fig. 3 is a schematic flow chart of steps of the biological 3D printing method in the embodiment of the present invention, where in the embodiment of the present invention, the method includes:
Further, referring to fig. 4, fig. 4 is a schematic flow chart of sub-steps of a biological 3D printing method according to an embodiment of the present invention, in the embodiment of the present invention, the calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field described in step 301 specifically includes:
and step 402, setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to an acoustic wave equation.
The method principle adopted by the biological 3D printing method is consistent with the principle adopted by the biological 3D printing system 100 in the above embodiment, and specifically, reference may be made to the description of the biological 3D printing system 100 in the above embodiment, which is not repeated herein.
Compared with the prior art, the biological 3D printing method provided by the invention does not have the nozzle in the traditional method, but adopts the acoustic control technology, and the acoustic control technology has the advantages of non-contact and no damage, so that the acoustic control technology does not damage cells, can effectively ensure the bioactivity of the cells, and can also control the cells in parallel, so that the printing based on the surface or the three-dimensional can be realized except the traditional printing based on the point, and the printing speed is higher; meanwhile, because the sound field can generate stress and deformation on cells in the sound field, the invention can also provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and ensure that the three-dimensional complex structure assembled by discrete cells is fixedly formed, thereby avoiding the damage problem to the cells during cross-linking forming.
In the several embodiments provided in this application, it should be understood that the disclosed systems and methods may be implemented in other ways. For example, the system embodiments described above are merely illustrative, e.g., the division of the modules is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple modules or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with respect to each other may be through some interface, indirect coupling or communication connection of systems or modules, electrical, mechanical, or other form.
The modules described as separate components may or may not be physically separate, and components shown as modules may or may not be physical modules, i.e., may be located in one place, or may be distributed over a plurality of network modules. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional module in each embodiment of the present invention may be integrated into one processing module, or each module may exist alone physically, or two or more modules may be integrated into one module. The integrated modules may be implemented in hardware or in software functional modules.
The integrated modules, if implemented in the form of software functional modules and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied essentially or in part or all of the technical solution or in part in the form of a software product stored in a storage medium, including instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to perform all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk, or an optical disk, or other various media capable of storing program codes.
It should be noted that, for the sake of simplicity of description, the foregoing method embodiments are all expressed as a series of combinations of actions, but it should be understood by those skilled in the art that the present invention is not limited by the order of actions described, as some steps may be performed in other order or simultaneously in accordance with the present invention. Further, those skilled in the art will appreciate that the embodiments described in the specification are all preferred embodiments, and that the acts and modules referred to are not necessarily all required for the present invention.
In the foregoing embodiments, the descriptions of the embodiments are emphasized, and for parts of one embodiment that are not described in detail, reference may be made to the related descriptions of other embodiments.
The foregoing is a description of a biological 3D printing system and method according to the present invention, and is not to be construed as limiting the invention, since modifications in terms of the detailed description and the application of the invention will be apparent to those skilled in the art upon review of the teachings of the embodiments of the invention.
Claims (7)
1. The biological 3D printing system is characterized by comprising a medical imaging module, a sound field inversion module, an artificial intelligence module, an acoustic control module and a water tank;
the medical imaging module is used for imaging human tissues to obtain tissue slice images;
the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
the artificial intelligent module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
the sound control module is used for transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspension cells in the water tank by utilizing the sound field, and constructing a biological tissue structure corresponding to the human tissue;
the sound field inversion module is specifically used for:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form a tissue pattern;
setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation;
the water tank is a region for 3D printing, the water tank is filled with biological ink containing suspension cells and growth factors, and the suspension cells are synthesized and assembled in the water tank by utilizing a sound field to form a specific biological tissue structure;
and the flow control module enables suspended cells in the water tank to be uniformly distributed in the silent field space.
2. The biological 3D printing system of claim 1, in which the acoustic steering module includes an array ultrasound transducer, a signal generator, and a power amplifier;
the array ultrasonic transducer is an ultrasonic transducer based on a surface array or an ultrasonic transducer based on a ring array, the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal.
3. The biological 3D printing system of claim 2, in which the acoustic steering module further comprises a beam combiner;
each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel in the beam synthesizer, the signal generator and the power amplifier.
4. A biological 3D printing system as claimed in claim 3, in which the array ultrasound transducer is located in the water tank.
5. The biological 3D printing system of claim 4, further comprising a flow control module for uniformly distributing suspended cells within the tank in a silent field space.
6. The biological 3D printing system of claim 5, in which the flow control module includes a water pump located at the bottom of the water tank and a control circuit that controls the water pump to control the uniform distribution of suspended cells within the water tank in silent field space.
7. A method of biological 3D printing, the method comprising:
imaging human tissue to obtain a tissue slice image;
taking the obtained tissue slice image as a target sound field, and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspension cells in a water tank by utilizing the sound field, and constructing a biological tissue structure corresponding to the human tissue;
the ultrasonic pulse sequence corresponding to the target sound field is calculated and synthesized, and the method comprises the following steps:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form a tissue pattern;
setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation;
the water tank is a region for 3D printing, the water tank is filled with biological ink containing suspension cells and growth factors, and the suspension cells are synthesized and assembled in the water tank by utilizing a sound field to form a specific biological tissue structure.
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| CN112776325B (en) * | 2020-12-29 | 2022-10-11 | 杭州电子科技大学 | Three-dimensional ultrasonic array support-free cell printing device and printing process thereof |
| CN113604463B (en) * | 2021-07-30 | 2023-02-24 | 深圳康沃先进制造科技有限公司 | Cell assembly method for Faraday wave multi-wavelength synthesis and application |
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