CN111826262A - Biological 3D printing system and method - Google Patents
Biological 3D printing system and method Download PDFInfo
- Publication number
- CN111826262A CN111826262A CN201910313928.8A CN201910313928A CN111826262A CN 111826262 A CN111826262 A CN 111826262A CN 201910313928 A CN201910313928 A CN 201910313928A CN 111826262 A CN111826262 A CN 111826262A
- Authority
- CN
- China
- Prior art keywords
- target
- sound field
- biological
- pulse sequence
- module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y99/00—Subject matter not provided for in other groups of this subclass
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/25—Process efficiency
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Abstract
The invention discloses a biological 3D printing system and a biological 3D printing method, wherein the system comprises a medical imaging module, a sound field inversion module, an artificial intelligence module, a sound control module and a water tank, wherein the medical imaging module is used for imaging human tissues to obtain tissue slice images; the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating an ultrasonic pulse sequence corresponding to the synthesized target sound field; the artificial intelligence module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence; the acoustic control module is used for transmitting a target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to human tissues. The invention can effectively ensure the biological activity of cells and avoid the damage to the cells during cross-linking molding.
Description
Technical Field
The invention relates to the technical field of cell printing, in particular to a biological 3D printing system and method.
Background
Biological 3D printing is a technology which can position and assemble biological materials or cell units according to the additive manufacturing principle under the drive of a digital three-dimensional model to manufacture products such as tissue engineering scaffolds, tissue organs and the like, and can effectively solve the problem that the source of transplanted organs is limited at present.
In the aspect of the current biological 3D printing technology, the biggest difficulty is represented by cell activity and cross-linking molding. Firstly, in terms of cell activity, for the printing modes of a liquid drop ejection type and an extrusion molding type, the common characteristic of the liquid drop ejection type and the extrusion molding type is that a nozzle is arranged, and the cell activity of the printing mode with the nozzle is always a difficult problem. In the aspect of crosslinking molding, the crosslinking molding is to fix and mold the printed bio-ink pattern by means of temperature control, chemical treatment, ultraviolet irradiation, and the like, but these crosslinking methods may damage cells.
Disclosure of Invention
The application provides a biological 3D printing system and method, which can solve the technical problems that the biological 3D printing technology in the prior art is difficult to ensure the activity of cells and easily damages the cells during cross-linking molding.
Specifically, the invention provides a biological 3D printing system in a first aspect, which comprises a medical imaging module, a sound field inversion module, an artificial intelligence module, a sound control module and a water tank;
the medical imaging module is used for imaging human tissues to obtain tissue slice images;
the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
the artificial intelligence module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
the acoustic control module is used for transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to the human tissue.
Preferably, the sound field inversion module is specifically configured to:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form tissue patterns;
and setting the intensity of a target space position point source according to the gray scale of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave motion equation.
Preferably, the acoustic steering module comprises an array ultrasonic transducer, a signal generator and a power amplifier;
the array ultrasonic transducer is an area array based ultrasonic transducer or a ring array based ultrasonic transducer, the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal.
Preferably, the acoustic steering module further comprises a beam synthesizer;
each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel in the beam synthesizer, the signal generator and the power amplifier.
Preferably, the arrayed ultrasound transducer is located in the water tank.
Preferably, the water tank contains bio-ink containing suspended cells and growth factors.
Preferably, the biological 3D printing system further comprises a flow control module for uniformly distributing the suspended cells in the water tank in the soundless field space.
Preferably, the flow control module comprises a water pump and a control circuit, the water pump is positioned at the bottom of the water tank, and the control circuit controls the water pump to control the suspension cells in the water tank to be uniformly distributed in the soundless field space.
The invention provides a biological 3D printing method in a second aspect, which comprises the following steps:
imaging human tissues to obtain tissue slice images;
taking the obtained tissue slice image as a target sound field, and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
and transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to the human tissue.
Preferably, the calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field includes:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form tissue patterns;
and setting the intensity of a target space position point source according to the gray scale of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave motion equation.
Compared with the prior art, the biological 3D printing system provided by the invention does not have a nozzle in the traditional method, but adopts an acoustic control technology, and the acoustic control technology has the advantages of non-contact and no damage, so that the cells are not damaged, the biological activity of the cells can be effectively ensured, and the cells can be controlled in parallel, therefore, in addition to the traditional printing based on points, the printing based on a surface or a three-dimensional can be realized, and the printing speed is higher; meanwhile, the sound field can generate stress and deformation on cells in the sound field, so that the invention can provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and fix and form the three-dimensional complex structure assembled by the discrete cells, thereby avoiding the problem of damage to the cells during cross-linking and forming.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a biological 3D printing system according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of another embodiment of a biological 3D printing system according to the present invention;
FIG. 3 is a schematic flow chart illustrating steps of a biological 3D printing method according to an embodiment of the present invention;
fig. 4 is a flow chart illustrating sub-steps of a biological 3D printing method according to an embodiment of the present invention.
Detailed Description
In order to make the objects, features and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, fig. 1 is a schematic structural diagram of a biological 3D printing system according to an embodiment of the present disclosure, in which a biological 3D printing system 100 includes a medical imaging module 101, a sound field inversion module 102, an artificial intelligence module 103, a sound control module 104, and a water tank 105.
The medical Imaging module 101 is configured to image a human tissue to obtain a tissue slice image, that is, large data of a biological tissue structure, and the Imaging mode may be MRI (Magnetic Resonance Imaging) or ultrasound Imaging.
The sound field inversion module 102 is configured to use the obtained tissue slice image as a target sound field, and calculate and synthesize an ultrasonic pulse sequence corresponding to the target sound field. Wherein the ultrasonic pulse sequence is an ultrasonic pulse sequence emitted by an array ultrasonic transducer of a target sound field.
The artificial intelligence module 103 is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence.
The Deep neural network learning model can adopt a conventional Deep Neural Network (DNN) model, the neural network layers in the DNN model can be divided into three types, namely an input layer, a hidden layer and an output layer, generally speaking, the first layer is the input layer, the last layer is the output layer, and the middle layers are all hidden layers.
The acoustic control module 104 is configured to transmit a target ultrasonic pulse sequence, establish a sound field corresponding to the target three-dimensional structure digital model, capture suspended cells in the water tank 105 using the sound field, and construct a biological tissue structure corresponding to the human tissue.
Specifically, the suspended cells are captured by using the acoustic force effect generated by the sound field established in the space, the biological tissue structure corresponding to the precise three-dimensional structure digital model is established two-dimensionally layer by layer or three-dimensionally integrally, and then the three-dimensional structure assembled by the dispersed suspended cells through acoustic control is fixed and formed through a natural growth mode.
The water tank 105 is an area for 3D printing, the water tank 105 is filled with bio-ink containing suspension cells and growth factors, and the suspension cells can be synthesized and assembled in the water tank 105 to form a specific biological tissue structure by using an acoustic field. Specifically, the water tank 105 is made of a biocompatible material such as medical stainless steel and quartz glass.
In particular, the sound field inversion module 102 is specifically configured to:
arranging and distributing the virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form tissue patterns; according to the gray scale of the tissue pattern, the intensity of a target space position point source is set, and the ultrasonic pulse sequence is reversely calculated according to the sound wave motion equation.
The sound wave equation is derived from a continuous equation and a momentum equation, and describes an important partial differential equation of sound wave propagation in a medium.
Compared with the prior art, the biological 3D printing system 100 provided by the embodiment of the invention does not have a nozzle in the traditional method, but adopts an acoustic control technology instead of the nozzle in the traditional method, and the acoustic control technology has the advantages of non-contact and no damage, so that the cells are not damaged, the biological activity of the cells can be effectively ensured, and the cells can be controlled in parallel, so that plane-based or three-dimensional-based printing can be realized in addition to the traditional point-based printing, and the printing speed is higher; meanwhile, the sound field can generate stress and deformation on cells in the sound field, so that the invention can provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and fix and form the three-dimensional complex structure assembled by the discrete cells, thereby avoiding the problem of damage to the cells during cross-linking and forming.
Further, based on the above embodiments, in the embodiment of the present invention, the acoustic manipulation module 104 includes an array ultrasonic transducer, a signal generator, and a power amplifier. The array ultrasonic transducer can be an ultrasonic transducer based on a planar array or an ultrasonic transducer based on a ring array; the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal. In addition, the transmission signal of the signal generator may be a continuous sinusoidal signal, or may be a pulsed sinusoidal signal.
The function of the array ultrasonic transducer is to convert the input electric power into mechanical power (i.e. ultrasonic wave) and transmit the mechanical power, and a small part of the power is consumed by the array ultrasonic transducer.
For better understanding of the present invention, embodiments of the present invention provide an arrayed ultrasonic transducer that may be comprised of a housing, a matching layer, a piezoelectric ceramic area array transducer, a backing, and an outgoing cable. The piezoelectric ceramic area array transducer is made of PZT-5 piezoelectric material polarized in the thickness direction. The piezoelectric ceramic area array transducer has 4 blocks, and each area array has 4096 array elements. Piezoelectric ceramic area array transducers are used as basic ultrasonic transducers from which ultrasonic signals are transmitted or received. The received ultrasonic signals can carry out ultrasonic imaging on the printed organ and observe the anatomical structure and growth condition of the organ in real time. The imaging mode can be a two-dimensional B mode image obtained based on a traditional pulse echo mode, an ultrasonic CT two-dimensional image can also be obtained in a transmission mode, a three-dimensional image can be reconstructed through the two-dimensional image, or the three-dimensional image can be directly obtained through three-dimensional volume data.
Further, the acoustic steering module 104 also includes a beam synthesizer. Each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel in the beam synthesizer, the signal generator and the power amplifier. The beam synthesizer is used for controlling the amplitude and the phase of the pulse signal transmitted by the array ultrasonic transducer, and the control mode can be realized by software or hardware.
Wherein the array ultrasonic transducer is positioned in the water tank 105, so that the suspended cells in the water tank 105 can be captured by using the sound field.
In the biological 3D printing system 100 provided by the embodiment of the present invention, the acoustic manipulation module 104 includes an array ultrasonic transducer, a signal generator and a power amplifier, wherein the array ultrasonic transducer may be an ultrasonic transducer based on a planar array or an ultrasonic transducer based on a ring array, so that the acoustic manipulation module 104 can be used to assemble cells into a two-dimensional or three-dimensional complex structure.
Further, referring to fig. 2 based on the above embodiment, fig. 2 is another schematic structural diagram of a biological 3D printing system according to an embodiment of the present invention, and in an embodiment of the present invention, a biological 3D printing system 100 includes a medical imaging module 101, a sound field inversion module 102, an artificial intelligence module 103, a sound manipulation module 104, a water tank 105, and a flow control module 106.
The flow control module 106 is used for enabling the suspended cells in the water tank 105 to be uniformly distributed in the soundless field space.
It can be understood that, when the suspended cells in the water tank 105 are captured by using the sound field, if the suspended cells in the water tank 105 are not uniformly distributed, the cells cannot be captured in the original cell capturing area, so that the capturing result is inaccurate, and the 3D printing requirement cannot be met. Therefore, in the embodiment of the present invention, in order to improve the accuracy of 3D printing, the flow control module 106 is used to uniformly distribute the suspended cells in the water tank 105 in the soundless field space.
Specifically, the flow control module 106 includes a water pump and a control circuit, wherein the water pump is located at the bottom of the water tank 105, and the control circuit controls the water pump to control the suspension cells in the water tank 105 to be uniformly distributed in the soundless field space. It should be noted that the number of the water pumps is at least one, and when the number of the water pumps is multiple, the control circuit controls the water pumps simultaneously, so that the water pumps are linked.
The biological 3D printing system 100 provided by the embodiment of the present invention further includes a flow control module 106, which can effectively improve the accuracy of 3D printing by maintaining the uniform distribution of the suspension cells in the water tank 105 in the soundless field space.
The biological 3D printing system 100 provided by the embodiment has the following main advantages:
(1) the nozzle in the traditional method is not adopted, but the acoustic control technology is adopted, and the acoustic control technology has the advantages of non-contact and no damage, so that the cells cannot be damaged, and the biological activity of the cells can be effectively ensured.
(2) And because the sound field pattern can be flexibly regulated and controlled through the ultrasonic pulse sequence, the invention can print more complex biological tissue structures.
(3) The cells can be manipulated in parallel, and surface-based or volume-based printing can be realized in addition to the traditional dot-based printing, so that the printing speed is higher.
(4) Because the sound field can generate stress and deformation on cells in the sound field, the invention can also provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and fix and shape the three-dimensional complex structure assembled by the discrete cells, thereby avoiding the problem of damage to the cells during cross-linking and shaping.
(5) The artificial intelligence module is utilized to learn the biological structure big data (tissue slice image) and the sound control big data (ultrasonic pulse sequence), so that the spatial resolution of three dimensions of the tissue slice image in the plane and between layers can be improved, the synthesis precision of a target sound field is improved, and finally, an accurate three-dimensional digital structure model and a corresponding ultrasonic emission pulse sequence are established.
(6) The cells can be assembled into two-dimensional or three-dimensional complex structures by using the acoustic manipulation module.
Further, an embodiment of the present invention further provides a biological 3D printing method, referring to fig. 3, where fig. 3 is a schematic flow chart of steps of the biological 3D printing method in the embodiment of the present invention, and in the embodiment of the present invention, the method includes:
301, imaging a human tissue to obtain a tissue slice image;
and 304, transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the digital model of the target three-dimensional structure, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to the human tissue.
Further, referring to fig. 4, fig. 4 is a schematic flow chart illustrating a sub-step of a biological 3D printing method according to an embodiment of the present invention, in the embodiment of the present invention, the calculating and synthesizing of the ultrasonic pulse sequence corresponding to the target sound field in step 301 specifically includes:
and 402, setting the intensity of a target space position point source according to the gray level of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave equation.
The method principle adopted by the biological 3D printing method is the same as the principle adopted by the biological 3D printing system 100 in the above embodiment, and specific reference may be made to the description of the biological 3D printing system 100 in the above embodiment, which is not described herein again.
Compared with the prior art, the biological 3D printing method provided by the invention does not have a nozzle in the traditional method, but adopts an acoustic control technology, and the acoustic control technology has the advantages of non-contact and no damage, so that the cells are not damaged, the biological activity of the cells can be effectively ensured, and the cells can be controlled in parallel, therefore, in addition to the traditional printing based on points, the printing based on a surface or a three-dimensional can be realized, and the printing speed is higher; meanwhile, the sound field can generate stress and deformation on cells in the sound field, so that the invention can provide a certain mechanical environment for the cells, promote the growth and fusion of the cells, and fix and form the three-dimensional complex structure assembled by the discrete cells, thereby avoiding the problem of damage to the cells during cross-linking and forming.
In the several embodiments provided in the present application, it should be understood that the disclosed system and method may be implemented in other ways. For example, the above-described system embodiments are merely illustrative, and for example, the division of the modules is merely a logical division, and in actual implementation, there may be other divisions, for example, multiple modules or components may be combined or integrated into another system, or some features may be omitted, or not implemented. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, systems or modules, and may be in an electrical, mechanical or other form.
The modules described as separate parts may or may not be physically separate, and parts displayed as modules may or may not be physical modules, may be located in one place, or may be distributed on a plurality of network modules. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of the present embodiment.
In addition, functional modules in the embodiments of the present invention may be integrated into one processing module, or each of the modules may exist alone physically, or two or more modules are integrated into one module. The integrated module can be realized in a hardware mode, and can also be realized in a software functional module mode.
The integrated module, if implemented in the form of a software functional module and sold or used as a stand-alone product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
It should be noted that, for the sake of simplicity, the above-mentioned method embodiments are described as a series of acts or combinations, but those skilled in the art should understand that the present invention is not limited by the described order of acts, as some steps may be performed in other orders or simultaneously according to the present invention. Further, those skilled in the art will appreciate that the embodiments described in the specification are presently preferred and that no acts or modules are necessarily required of the invention.
In the above embodiments, the descriptions of the respective embodiments have respective emphasis, and for parts that are not described in detail in a certain embodiment, reference may be made to related descriptions of other embodiments.
While the present invention has been described with reference to a 3D printing system and method, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A biological 3D printing system is characterized by comprising a medical imaging module, a sound field inversion module, an artificial intelligence module, a sound control module and a water tank;
the medical imaging module is used for imaging human tissues to obtain tissue slice images;
the sound field inversion module is used for taking the obtained tissue slice image as a target sound field and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
the artificial intelligence module is used for inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
the acoustic control module is used for transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to the human tissue.
2. The biological 3D printing system of claim 1, wherein the sound field inversion module is specifically configured to:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form tissue patterns;
and setting the intensity of a target space position point source according to the gray scale of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave motion equation.
3. The biological 3D printing system of claim 1, wherein the acoustic steering module comprises an arrayed ultrasound transducer, a signal generator, and a power amplifier;
the array ultrasonic transducer is an area array based ultrasonic transducer or a ring array based ultrasonic transducer, the signal generator is used for generating an electric signal for exciting the array ultrasonic transducer, and the power amplifier is used for amplifying the electric signal.
4. The biological 3D printing system of claim 3, wherein the acoustic steering module further comprises a beam synthesizer;
each array element on the array ultrasonic transducer corresponds to one transmitting/receiving channel in the beam synthesizer, the signal generator and the power amplifier.
5. The biological 3D printing system of claim 4, wherein the arrayed ultrasound transducer is located in the water tank.
6. The biological 3D printing system of any one of claims 1 to 5, wherein the water tank includes a bio-ink containing suspended cells and growth factors.
7. The biological 3D printing system of claim 6, further comprising a flow control module for uniformly distributing the suspended cells in the water tank in the soundless field space.
8. The biological 3D printing system according to claim 7, wherein the flow control module comprises a water pump and a control circuit, the water pump is located at the bottom of the water tank, and the control circuit controls the water pump to control the suspension cells in the water tank to be uniformly distributed in the soundless field space.
9. A biological 3D printing method, characterized in that the method comprises:
imaging human tissues to obtain tissue slice images;
taking the obtained tissue slice image as a target sound field, and calculating and synthesizing an ultrasonic pulse sequence corresponding to the target sound field;
inputting the tissue slice image and the ultrasonic pulse sequence into a preset deep neural network learning model to obtain a target three-dimensional structure digital model and a target ultrasonic pulse sequence;
and transmitting the target ultrasonic pulse sequence, establishing a sound field corresponding to the target three-dimensional structure digital model, capturing suspended cells in the water tank by using the sound field, and constructing a biological tissue structure corresponding to the human tissue.
10. The biological 3D printing method according to claim 9, wherein the computing and synthesizing the ultrasonic pulse sequence corresponding to the target sound field comprises:
arranging and distributing virtual point sources according to the tissue slice images by taking the tissue slice images as a target sound field to form tissue patterns;
and setting the intensity of a target space position point source according to the gray scale of the tissue pattern, and reversely calculating the ultrasonic pulse sequence according to a sound wave motion equation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313928.8A CN111826262B (en) | 2019-04-18 | 2019-04-18 | Biological 3D printing system and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313928.8A CN111826262B (en) | 2019-04-18 | 2019-04-18 | Biological 3D printing system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111826262A true CN111826262A (en) | 2020-10-27 |
| CN111826262B CN111826262B (en) | 2023-06-20 |
Family
ID=72914943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910313928.8A Active CN111826262B (en) | 2019-04-18 | 2019-04-18 | Biological 3D printing system and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111826262B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112776325A (en) * | 2020-12-29 | 2021-05-11 | 杭州电子科技大学 | Three-dimensional ultrasonic array support-free cell printing device and printing process thereof |
| CN113604463A (en) * | 2021-07-30 | 2021-11-05 | 武汉大学 | Cell assembly method for Faraday wave multi-wavelength synthesis and application |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104224277A (en) * | 2013-06-18 | 2014-12-24 | 三星电子株式会社 | Method, apparatus and system for generating ultrasound |
| US20160184790A1 (en) * | 2010-03-12 | 2016-06-30 | Los Alamos National Security, Llc | Material fabrication using acoustic radiation forces |
| US20170217102A1 (en) * | 2016-01-29 | 2017-08-03 | Siemens Medical Solutions Usa, Inc. | Multi-Modality Image Fusion for 3D Printing of Organ Morphology and Physiology |
| CN107249859A (en) * | 2014-12-19 | 2017-10-13 | 马克斯-普朗克科学促进学会 | The increasing material manufacturing method and apparatus for being used to manufacture part of precursor material is positioned using sound power |
| RU2654199C1 (en) * | 2017-07-18 | 2018-05-16 | Самсунг Электроникс Ко., Лтд. | Segmentation of human tissues in computer image |
| CN108697373A (en) * | 2016-02-17 | 2018-10-23 | 皇家飞利浦有限公司 | Physics 3D anatomical structure models manufacture |
| CN208090895U (en) * | 2018-01-25 | 2018-11-13 | 广州迈普再生医学科技股份有限公司 | A kind of humidity control system of biology 3D printer enclosed environment |
-
2019
- 2019-04-18 CN CN201910313928.8A patent/CN111826262B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160184790A1 (en) * | 2010-03-12 | 2016-06-30 | Los Alamos National Security, Llc | Material fabrication using acoustic radiation forces |
| CN104224277A (en) * | 2013-06-18 | 2014-12-24 | 三星电子株式会社 | Method, apparatus and system for generating ultrasound |
| CN107249859A (en) * | 2014-12-19 | 2017-10-13 | 马克斯-普朗克科学促进学会 | The increasing material manufacturing method and apparatus for being used to manufacture part of precursor material is positioned using sound power |
| US20170217102A1 (en) * | 2016-01-29 | 2017-08-03 | Siemens Medical Solutions Usa, Inc. | Multi-Modality Image Fusion for 3D Printing of Organ Morphology and Physiology |
| CN108697373A (en) * | 2016-02-17 | 2018-10-23 | 皇家飞利浦有限公司 | Physics 3D anatomical structure models manufacture |
| RU2654199C1 (en) * | 2017-07-18 | 2018-05-16 | Самсунг Электроникс Ко., Лтд. | Segmentation of human tissues in computer image |
| CN208090895U (en) * | 2018-01-25 | 2018-11-13 | 广州迈普再生医学科技股份有限公司 | A kind of humidity control system of biology 3D printer enclosed environment |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112776325A (en) * | 2020-12-29 | 2021-05-11 | 杭州电子科技大学 | Three-dimensional ultrasonic array support-free cell printing device and printing process thereof |
| CN113604463A (en) * | 2021-07-30 | 2021-11-05 | 武汉大学 | Cell assembly method for Faraday wave multi-wavelength synthesis and application |
| CN113604463B (en) * | 2021-07-30 | 2023-02-24 | 深圳康沃先进制造科技有限公司 | Cell assembly method for Faraday wave multi-wavelength synthesis and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111826262B (en) | 2023-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Marzo et al. | Ultraino: An open phased-array system for narrowband airborne ultrasound transmission | |
| JP5276336B2 (en) | Method and apparatus for creating an ultrasound image of a moving object using a deformable model | |
| Demi | Practical guide to ultrasound beam forming: Beam pattern and image reconstruction analysis | |
| Roux et al. | 2-D ultrasound sparse arrays multidepth radiation optimization using simulated annealing and spiral-array inspired energy functions | |
| Ho et al. | Wall-less flow phantoms with tortuous vascular geometries: Design principles and a patient-specific model fabrication example | |
| CN111826262B (en) | Biological 3D printing system and method | |
| Rasmussen et al. | 3-D ultrasound imaging performance of a row-column addressed 2-D array transducer: A measurement study | |
| CN103315769B (en) | Diagnostic ultrasound equipment, image processing apparatus and image processing method | |
| CN107530061A (en) | Synthetic aperture ultrasonic system | |
| US20210169340A1 (en) | Method for Realizing Arbitrary Ultrasonic Field | |
| Yazdi et al. | A novel fabrication method for compliant silicone phantoms of arterial geometry for use in particle image velocimetry of haemodynamics | |
| EP3754558A1 (en) | Method and system for generating a synthetic elastrography image | |
| Alles et al. | A reconfigurable all-optical ultrasound transducer array for 3D endoscopic imaging | |
| Stuart et al. | Real-time volumetric synthetic aperture software beamforming of row–column probe data | |
| Lee et al. | Deep learning-based framework for fast and accurate acoustic hologram generation | |
| Onofrei et al. | On the synthesis of acoustic sources with controllable near fields | |
| Bilbao et al. | Modeling continuous source distributions in wave-based virtual acoustics | |
| CN105877778A (en) | Beam Forming Apparatus, Method For Forming Beams, Ultrasonic Imaging Apparatus, And Ultrasonic Probe | |
| Luo et al. | Rapid quantitative imaging of high intensity ultrasonic pressure fields | |
| Sobhani et al. | Ultrafast orthogonal row–column electronic scanning (uFORCES) with bias-switchable top-orthogonal-to-bottom electrode 2-D arrays | |
| Jensen et al. | 8a-3 system architecture of an experimental synthetic aperture real-time ultrasound system | |
| WO2020211048A1 (en) | Biological 3d printing system and method | |
| Zeng et al. | Neural Born series operator for biomedical ultrasound computed Tomography | |
| JP2009279158A (en) | Volume data processor | |
| Sharifzadeh et al. | An ultra-fast method for simulation of realistic ultrasound images |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |