CN111820202B - Method for performing dynamic air-conditioned insecticidal action on traditional Chinese medicinal materials by using air-conditioned cold store - Google Patents

Abstract

The invention relates to a method for performing dynamic air-conditioned insecticidal action on traditional Chinese medicinal materials by using an air-conditioned cold store, belonging to the technical field of insecticidal preservation of traditional Chinese medicinal materials. The invention provides a method for dynamically regulating the atmosphere of Chinese medicinal materials to kill insects by utilizing an atmosphere-controlled warehouse, which is characterized in that nitrogen or carbon dioxide is dynamically filled into the warehouse for storing the Chinese medicinal materials to create a low-oxygen environment meeting the requirement, and the environmental temperature, humidity and operation time are controlled to realize the purposes of insect prevention, insect killing, mildew prevention and oxidation prevention for the Chinese medicinal materials. The method for dynamically killing the pests in the air-conditioned cold store by the air-conditioned cold store can effectively ensure the quality of the traditional Chinese medicinal materials and the products thereof on the basis of realizing the pests in an environment-friendly and efficient manner and reducing the risk of microorganisms.

Description

Method for performing dynamic air-conditioned insecticidal action on traditional Chinese medicinal materials by using air-conditioned cold store

Technical Field

The invention relates to a method for performing dynamic air-conditioned insecticidal action on traditional Chinese medicinal materials by using an air-conditioned cold store, belonging to the technical field of insecticidal preservation of traditional Chinese medicinal materials.

Background

The traditional Chinese medicine is a medicine which is collected, processed and used under the guidance of the traditional Chinese medicine theory, the main source of the traditional Chinese medicine is natural products such as plants, animals and the like, the traditional Chinese medicine has various varieties, and the traditional Chinese medicine can be divided into various types such as animals, rhizomes, leaves, flowers, fruits, whole plants and the like according to different medicine parts. The traditional Chinese medicine is easily affected by external environment, and is easy to cause the deterioration of quality, such as insect generation, mildew, oxidative deterioration and the like, which greatly affects the medicinal value, the commodity value and the production and manufacture of subsequent products of the traditional Chinese medicine.

The Chinese medicinal insect pest is mainly caused by exogenous invasion or endogenous hatching of Lepidoptera (Lepidoptera) and Coleoptera (Coleoptera). The insect pest directly influences the appearance of the medicinal materials, and also indirectly causes the quality of the medicinal materials to be more easily influenced, thereby reducing the quality. At present, toxic medicaments (traditional insecticides such as sulfur, aluminum phosphide, methyl bromide and the like) are mainly used for fumigating to eliminate insect pests, most of imagoes can be killed by the method, but the killing effect on insect eggs is limited, secondary insect pests easily appear in varieties with insect pests due to high probability of embedding the insect eggs, and at present, an effective mode for dealing with insect egg threats is not available temporarily. The reagents are toxic reagents, wherein methyl bromide is three carcinogens and has destructiveness to an ozone layer, the reagent is completely forbidden in 2015 according to the Montreal protocol Gopoli correction case, and the existing toxic reagents are gradually banned along with the soundness of national regulation and regulations, so that the application of the existing method in drug disinfestation is seriously influenced.

The mildew is mainly expressed as the overproof of microorganisms, which is induced to be aggravated by the pollution of samples in the production and preservation processes, over-high humidity, poor ventilation environment and other conditions. Wherein, the Aspergillus flavus is a common strain, and the generated aflatoxin is a carcinogen and has destructive effect on human and animal liver tissues. 2015 edition of Chinese pharmacopoeia requires that 19 varieties of aflatoxin B1 such as peach kernel and the like are not more than 5 mu g/kg; the total amount of aflatoxin B1, aflatoxin G2, aflatoxin G1 and aflatoxin B2 is not more than 10 μ G/kg. Since most of the molds are aerobic bacteria, the mold is controlled or delayed mainly by adding chemical agents such as mold removing agents and oxygen consuming agents or by using mechanical equipment for cooking sterilization in the industry at present, but additional cost is generated.

Oxidative deterioration refers to a phenomenon occurring in the preservation process of medicinal materials, but due to the self properties or high water activity of samples and other reasons, oil leakage and other phenomena easily occur in part of samples, the phase and quality of the medicinal materials are affected, and the oxidative deterioration is usually dealt with by adopting a low-temperature storage mode at present, but the preservation cost is high.

The quality of the traditional Chinese medicinal materials used as special commodities for medical care needs to be well guaranteed and supervised. At present, part of domestic enterprises carry out air conditioning treatment by using an oxygen consumption agent in a sealed package or a low-temperature low-oxygen air conditioning warehouse, for example, a patent with the patent application number of 201310304524.5, the method can simultaneously take insect damage, mildew damage and oxidative deterioration into consideration, but the method needs a longer time to reach a lower oxygen concentration in the early stage, and has the defects of long period, large oxygen concentration fluctuation, large packaging workload, easy package breakage and the like due to principle limitation. The gas replacement method is applied in the fields of cultural relics preservation and vegetable and fruit preservation in China, but relates to special research and method for traditional Chinese medicines, processed products and processed products thereof.

Disclosure of Invention

The invention aims to overcome the defects of the prior art, and provides a method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage aiming at different varieties of traditional Chinese medicinal materials.

In order to achieve the purpose, the invention adopts the technical scheme that: a method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage comprises the following steps:

(1) putting the traditional Chinese medicinal materials to be treated into a warehouse, and sealing the warehouse;

(2) and (2) constructing a low-oxygen environment in the warehouse in the step (1), controlling the temperature and the humidity in the warehouse, performing controlled atmosphere treatment, and then controlling the time of the controlled atmosphere treatment, so that the purpose of performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere warehouse can be realized.

The invention provides a method for dynamically regulating the atmosphere of Chinese medicinal materials to kill insects by utilizing an atmosphere-controlled warehouse, which is characterized in that nitrogen or carbon dioxide is dynamically filled into the warehouse for storing the Chinese medicinal materials to create a low-oxygen environment meeting the requirement, and the environmental temperature, humidity and operation time are controlled to realize the purposes of insect prevention, insect killing, mildew prevention and oxidation prevention for the Chinese medicinal materials.

As a preferred embodiment of the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere storage, in the step (1), the traditional Chinese medicinal materials are placed into a warehouse in a stacking mode, and the warehouse is a soft warehouse or a sealed warehouse with air tightness meeting the requirement.

The invention provides a method for performing dynamic air-conditioned disinsection on traditional Chinese medicinal materials by using an air-conditioned warehouse.

As a preferred embodiment of the method for performing dynamic modified atmosphere disinsection on traditional Chinese medicinal materials by using a modified atmosphere storage, in the step (2), the volume concentration of oxygen in the low-oxygen environment is 0.1-5%; more preferably, the oxygen is present in a concentration of 0.5 to 3% by volume.

As a preferred embodiment of the method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage, the creation of the low-oxygen environment is realized by dynamically filling nitrogen or carbon dioxide.

As a preferred embodiment of the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere storage, in the step (2), the temperature is 20-40 ℃; more preferably, the temperature is from 25 ℃ to 35 ℃.

As a preferred embodiment of the method for performing dynamic modified atmosphere disinsection on the traditional Chinese medicinal materials by using the modified atmosphere storage, in the step (2), the humidity is 25-90%; more preferably, the humidity is 40-80%.

As a preferred embodiment of the method for performing dynamic modified atmosphere disinsection on the traditional Chinese medicinal materials by using the modified atmosphere storage, in the step (2), the modified atmosphere treatment time is 1-6 weeks according to different types of the traditional Chinese medicines to be treated; more preferably, the time of the modified atmosphere treatment is 2-4 weeks.

As a preferred embodiment of the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere storage, after the controlled atmosphere treatment in the step (2) is finished, the storage is opened, ventilation is performed for 5-20min, and when the oxygen volume concentration outside the storage is the same as the oxygen volume concentration in the storage, workers and equipment can enter the storage and remove the traditional Chinese medicinal materials for subsequent production operation.

The invention provides a method for performing dynamic air-conditioned disinsection on traditional Chinese medicinal materials by using an air-conditioned cold store, which comprises the following steps of (2) setting air-conditioned parameters (the air-conditioned parameters comprise oxygen volume concentration, temperature, humidity and air-conditioned processing time) aiming at different types of traditional Chinese medicinal materials, and achieving the purpose of performing dynamic air-conditioned disinsection on the traditional Chinese medicinal materials by using the air-conditioned cold store; after the air-conditioning insecticidal treatment is finished, opening the warehouse, ventilating for 5-20min according to the quantity of the goods, and ensuring that the oxygen concentration in the warehouse is raised back to a normal range, and then moving out the goods by personnel and equipment for subsequent production operation.

The method for dynamically air-conditioned disinsection of traditional Chinese medicinal materials by using the air-conditioned storehouse has obvious disinsection effect on various storehouse pests or pests invading the traditional Chinese medicinal materials, such as common medicinal material pests of Coleoptera (Coleoptera) Egyptidae (Anobiidae Fleming), Tylophoraceae (Tenebrionidae), Henry beetle (Curculonidae) and the like, common medicinal material pests of Lepidoptera (Lepidoteera) Meretricis (Gelecheidae), Philidae (Pyralidae), Chrysomyiamidae (Tineidae) and the like, can achieve the effect that the adult disinsection rate is 100%, and can achieve the effect that the occurrence rate is 0% when secondary pests are caused by hatching the eggs of the pests.

By utilizing the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by utilizing the controlled atmosphere storage, after the traditional Chinese medicinal materials such as rhizomes, flowers, leaves, fruits, seeds, animals and the like are treated, the appearance and the quality (index component types, content, appearance and the like) of the traditional Chinese medicinal materials are not influenced, and the content of aerobic microorganisms is obviously reduced or even disappears

Compared with the prior art, the invention has the beneficial effects that:

(1) the method for dynamically killing the pests in the air-conditioned cold store by the air-conditioned cold store can effectively ensure the quality of the traditional Chinese medicinal materials and the products thereof on the basis of realizing the pests in an environment-friendly and efficient manner and reducing the risk of microorganisms.

(2) The method for dynamically air-conditioned disinsection of the traditional Chinese medicinal materials by using the air-conditioned cold store can simultaneously promote the operations of disinsection, insect prevention, mildew prevention and oxidation prevention of large-batch and full-variety (including rhizomes, leaves, flowers, fruits, animals and the like) traditional Chinese medicinal materials and processed products thereof, has low labor cost, and can greatly improve the operation efficiency while ensuring the quality.

Drawings

FIG. 1 is a three-dimensional view of a dead elephant shape;

FIG. 2 is a three-view of the morphology of a killed cereal theft;

FIG. 3 shows the body morphology of dead insects in some of the test groups;

FIG. 4 is a graph showing the change of a part of the Chinese medicinal material TAMC (aerobic bacterial count) before and after the test in test example 2 of the present invention;

FIG. 5 is a graph showing the variation of a part of the traditional Chinese medicine TYMC (total number of mold yeasts) before and after the test in test example 2 of the present invention;

FIG. 6 is a graph showing the content change of a part of the diluted alcohol extract of a Chinese medicinal material before and after the test in test example 2 of the present invention;

FIG. 7 is a graph showing the content changes of some Chinese medicinal materials before and after the test in test example 2 of the present invention;

FIG. 8 is a comparison of the appearance of the cinnamon of the control group and the cinnamon of the test group according to the present invention in test example 2;

FIG. 9 is a comparison graph of the appearances of the citrus aurantium traditional Chinese medicinal materials of the control group and the test group in the test example 2 of the present invention;

FIG. 10 is a comparison graph of the appearance of the Gardenia jasminoides ellis of the present invention in test example 2, control group and test group;

FIG. 11 is a comparison of the appearance of the mint herb of the present invention in test example 2, control group and test group;

FIG. 12 is a comparison of the appearance of the rehmannia glutinosa as the reference and the test in test example 2 of the present invention;

FIG. 13 is a comparison of the appearance of the ginseng Chinese medicinal materials of the control group and the test group in the test example 2 of the present invention;

FIG. 14 is a comparison of the appearance of the dried ginger of the present invention in test example 2, the control group and the test group;

FIG. 15 is a comparison graph of the appearance of the caulis Sinomenii in the control group and the test group according to the present invention in test example 2.

Detailed Description

To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.

Example 1

The embodiment is a method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage, which comprises the following steps:

(1) putting the traditional Chinese medicinal materials to be treated, which do not contain volatile components and have low content of saccharide components, into a warehouse with air tightness meeting the requirement in a stacking mode, and sealing the warehouse;

(2) in the warehouse in the step (1), air-conditioning parameters are carried out on different types of traditional Chinese medicinal materials, a low-oxygen environment is created, the volume concentration of oxygen is 0.3 +/-0.2% by dynamically filling nitrogen, the temperature in the warehouse is controlled to be 35 +/-1 ℃ and the humidity in the warehouse is controlled to be 40 +/-10%, air-conditioning treatment is carried out, then the time of the air-conditioning treatment is controlled to be 2-4 weeks (determined according to varieties), and the purpose of carrying out dynamic air-conditioning disinsection on the traditional Chinese medicinal materials by using an air-conditioning warehouse can be achieved.

And (3) after the air conditioning treatment in the step (2) is finished, opening the warehouse, ventilating for 20min, and after the oxygen volume concentration outside the warehouse is the same as the oxygen volume concentration in the warehouse, enabling workers and equipment to enter the warehouse and move out the traditional Chinese medicinal materials to perform subsequent production operation.

The method can realize quick green disinsection of the traditional Chinese medicine without volatile components and with lower saccharide content: namely, live insects and worm eggs are killed in the shortest time on the premise of not influencing the quality of the traditional Chinese medicine.

Example 2

The embodiment is a method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage, which comprises the following steps:

(1) putting the traditional Chinese medicinal materials to be treated into a sealed warehouse in a stacking mode, and sealing the warehouse;

(2) in the warehouse in the step (1), air-conditioning parameters are carried out on different types of traditional Chinese medicinal materials, a low-oxygen environment is created, the volume concentration of oxygen is 1 +/-0.5% by dynamically filling nitrogen, the temperature in the warehouse is controlled to be 30 +/-3 ℃ and the humidity in the warehouse is controlled to be 50 +/-10%, air-conditioning treatment is carried out, then the time of the air-conditioning treatment is controlled for 2-4 weeks (determined according to varieties), and the purpose of carrying out dynamic air-conditioning disinsection on the traditional Chinese medicinal materials by using an air-conditioning warehouse can be achieved.

And (3) after the air conditioning treatment in the step (2) is finished, opening the warehouse, ventilating for 15min, and after the oxygen volume concentration outside the warehouse is the same as the oxygen volume concentration in the warehouse, enabling workers and equipment to enter the warehouse and move out the traditional Chinese medicinal materials to perform subsequent production operation.

The green disinsection of the traditional Chinese medicine capable of easily killing the insects is realized by adopting the embodiment: namely, live insects and worm eggs are killed in the normal production period of the traditional Chinese medicine on the premise of not influencing the quality of the traditional Chinese medicine.

Example 3

The test example is a method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage, which comprises the following steps:

(1) arranging an expanded software warehouse in a shade place, and connecting the expanded software warehouse with nitrogen generation equipment;

(2) putting the Chinese medicinal materials to be subjected to insecticidal treatment into a soft warehouse in a stacking manner, and sealing the warehouse;

(3) in the warehouse in the step (2), the air-conditioning parameters are carried out on different types of traditional Chinese medicinal materials, a low-oxygen environment is created, the volume concentration of oxygen is 3 +/-2% by dynamically filling nitrogen, the temperature in the warehouse is controlled to be 25 +/-5 ℃ and the humidity in the warehouse is controlled to be 60 +/-20%, air-conditioning treatment is carried out, then the time of the air-conditioning treatment is controlled to be 4-6 weeks (determined according to varieties), and the purpose of carrying out dynamic air-conditioning disinsection on the traditional Chinese medicinal materials by using the air-conditioning warehouse can be achieved.

And (4) after the air conditioning treatment in the step (3) is finished, opening the warehouse, ventilating for 5min, and when the oxygen volume concentration outside the warehouse is the same as the oxygen volume concentration in the warehouse, allowing workers and equipment to enter the warehouse and move out the traditional Chinese medicinal materials for subsequent production operation.

By adopting the embodiment, the modified atmosphere insecticidal can be carried out on all the traditional Chinese medicines which are easy to grow insects on the premise of not building a warehouse: namely, the construction and use cost is reduced on the premise of not influencing the quality of the traditional Chinese medicine.

Test example 1

In the experimental example, the method for performing the dynamic controlled-atmosphere insecticidal action on the traditional Chinese medicinal materials by using the warehouse disclosed by the invention is used for performing insecticidal tests on various traditional Chinese medicinal materials.

Test material and equipment

Materials: 8 types of 63 traditional Chinese medicine samples including cinnamon, tuckahoe, semen plantaginis, grifola, dwarf lilyturf tuber, rhizoma atractylodis, rehmannia root, mint leaf, peach kernel, ginseng, rhizoma cimicifugae, rhizoma anemarrhenae, dried ginger, moutan bark, spina date seed, fructus cannabis, gardenia, caulis sinomenii, safflower, radix bupleuri, immature bitter orange, mangnolia officinalis, radix puerariae, cicada shell, platycodon grandiflorum, astragalus mongholicus, betelnut, trichosanthes root, scutellaria baicalensis, asarum, black sesame, radix peucedani, akebia stem, Chinese date and the like; insects such as corn beetle (Tenebriodes mauritanicus), elephant (Sitophilus granaria), medicinal material A (Stegobium paniceum), Indian meal moth (Plodia interpunctella), and the like.

Equipment: a temperature and humidity detector (TR-72wf, T & D CO.), an oxygen detector (PAC7000, Draeger CO.);

second, test method

1. Preparing insects, grouping:

adult test: identifying the obtained insect sample according to the appearance characteristics to determine that the source of the test object is accurate; respectively placing the Golay and the Golay elephant into the traditional Chinese medicines until the Golay elephant and the traditional Chinese medicines grow stably, grouping 20 grain steams/group into glass bottles containing the same traditional Chinese medicines, and packaging 13 bottles in total. Randomly selecting 10 bottles (Guest Sagittarius numbers A-1 to A-10 and Guest Sagittarius numbers B-1 to B-10) as a test group, and 3 bottles as a control group (Guest Sagittarius numbers A-11 to A-13 and Guest Sagittarius numbers B-11 to B-13);

and (3) worm egg test: live insects of normally bred insect colonies containing the feed are removed, divided into 4 parts, and respectively filled into feeding boxes covered with gauze, wherein the two boxes are used as an insect egg hatching test group (the numbers of the grain robbers are A-20-A-21 and the numbers of the grain elephants are B-20-B-21), and the two boxes are used as a control group (the numbers of the grain robbers are A-22-A-23 and the numbers of the grain elephants are B-22-B-23).

2. Modified atmosphere treatment:

the test groups were placed at temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: 55 +/-10% environment; temperature at which the control group was placed: 25 ± 2 ℃, oxygen volume concentration 20.9%, humidity: 55 plus or minus 10 percent.

3. Data collection:

adult test: and (4) carrying out 2 weeks, observing the test group after the test is finished, placing the insects which stop moving in the natural environment for 2 days, confirming whether the insects are in a false death/dormancy state, and counting the insect mortality.

And (3) worm egg test: after the test is finished, each group is placed in an appropriate growth environment for 2 weeks, and whether adults hatch or not is observed.

4. And (3) data analysis: the test confirms the insecticidal effect under the test conditions.

Third, test results

The results of the adult test groups of the sitobira and the oryza incertulas after 2 weeks of the test treatment are shown in tables 1 and 2, respectively, and the results of the egg test groups of the sitobira incertulas and the oryza incertulas are shown in tables 3 and 4, respectively.

TABLE 1 results of the insecticidal treatment of Gularch by using the method of dynamic controlled atmosphere insecticidal of Chinese medicinal materials in controlled atmosphere storage

From the results in table 1 above, it can be seen that at the temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: after 2 weeks of treatment in 55 +/-10% environment, 10 groups of the test groups die of the sitaglipta millettiae, no false death occurs, and the death rate is 100%; control mortality was 5% -30%, demonstrating that at temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: the pesticide has good effect of killing the adult corn beetles under the environment condition of 55 +/-10 percent.

TABLE 2 insecticidal results of oryza sativa by using modified atmosphere storage method for dynamic modified atmosphere insecticidal treatment of Chinese medicinal materials

From the results of table 2 above, it can be seen that at temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: after 2 weeks of treatment in 55 +/-10% environment, 10 groups of trough elephants in the test group die, false death does not occur, and the death rate is 100%; control mortality was 10% -40%, demonstrating that at temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: the composition has good killing effect on the imagoes of the oryzophragmus chinensis under the environmental condition of 55 +/-10 percent.

FIG. 1 is a three-dimensional view of a dead elephant shape; FIG. 2 is a three-view of the morphology of a killed cereal theft; FIG. 3 shows the morphology of dead worms in some of the test groups.

TABLE 3 results of insecticidal treatment of the eggs of Gustolen by using the method of dynamic controlled atmosphere insecticidal of Chinese medicinal materials in controlled atmosphere storage

From the results of Table 3 above, it can be seen that at the temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: after 2 weeks of treatment in 55 ± 10% environment, no adults were present in the test group, i.e. the larvae i.e. eggs were completely dead, demonstrating that the modified atmosphere treatment was carried out at a temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: has good effect of killing the cereal stealing eggs under the environment condition of 55 +/-10 percent.

TABLE 4 insecticidal treatment results of the method for dynamic controlled-atmosphere insecticidal treatment of elephant eggs in controlled-atmosphere storage

From the results of Table 4 above, it can be seen that at the temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: after 2 weeks of treatment in 55 ± 10% environment, no adults were present in the test group, i.e. the larvae i.e. eggs were completely dead, demonstrating that the modified atmosphere treatment was carried out at a temperature: 25 ± 2 ℃, oxygen volume concentration: 0.3 ± 0.2%, humidity: the composition has good effect of killing the cereal weevil eggs under the environment condition of 55 +/-10 percent.

Test example 2: quality test of Chinese medicine

The experimental example prolongs the controlled atmosphere treatment time and confirms the influence of the controlled atmosphere treatment on indexes such as index components, extracts, microorganisms, appearance and the like of the traditional Chinese medicinal materials on the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere storage.

The test is carried out on 8 types of 63 Chinese medicinal samples, and the following examples are given.

First, materials and reagents: same as in test example 1

II, a test method:

1. sample preparation and grouping: sampling 6kg of each test variety by adopting a 5-point sampling method, uniformly mixing the obtained samples, and uniformly dividing the samples into a test group and a control group;

2. test parameters comprise 1 +/-0.5% of oxygen volume concentration, 30 +/-3 ℃ of controlled temperature, 50 +/-10% of humidity and 6 weeks;

3. the detector is arranged: a temperature and humidity detector (TR-72wf, T & D CO.), an oxygen detector (PAC7000, Draeger CO.);

4. and (3) sample recovery: recovering data of the sample and the detector after the test is finished;

5. sample detection: comparing all samples with appearance change, and performing qualitative and quantitative detection according to the method of the Chinese pharmacopoeia of Japanese medicine local prescription;

6. and (3) data analysis: and comparing the data difference between the samples before and after treatment, and evaluating the influence on the quality of the medicine under the treatment condition.

Third, test results

(1) According to the test method, the influence of the method for performing the dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere storage on the microbial content of part of the traditional Chinese medicinal materials is detected:

the test group was treated in an atmosphere of 1 + -0.5% oxygen volume concentration, 30 + -3 ℃ temperature and 50 + -10% humidity for 6 weeks, and the control group was stored in a cool-shade storehouse for 6 weeks. The results of comparing the TAMC (aerobic count) and TYMC (mold yeast count) of the test group and the control group after the completion of the test are shown in FIGS. 4 to 5, in which FIG. 4 is a graph showing the change in TAMC of a part of the samples before and after the test, and FIG. 5 is a graph showing the change in TYMC of a part of the samples before and after the test.

And (3) test results: as can be seen from the graphs in FIGS. 4 and 5, after the method for performing dynamic modified atmosphere insecticidal action on traditional Chinese medicinal materials by using the modified atmosphere storage of the invention, TAMC (aerobic bacteria total number) and TYMC (mold yeast total number) in 17 varieties show a descending trend, detection values of part of samples before and after test are less than 500, TAMC of bupleurum falcatum and rehmannia glutinosa are reduced by 2 orders of magnitude, and TYMC of cimicifuga foetida and Atractylodes lancea are reduced by 2 orders of magnitude, which proves that conditions (oxygen volume concentration of 1 +/-0.5%, temperature control of 30 +/-3 ℃ and humidity of 50 +/-10%) set by modified atmosphere parameters can generate inhibition effect on microorganisms in the method for performing dynamic modified atmosphere insecticidal action on traditional Chinese medicinal materials by using the modified atmosphere storage of the invention.

(2) According to the test method, the influence of the method for performing dynamic controlled atmosphere disinsection on the traditional Chinese medicine by using the controlled atmosphere storage on the content of the dilute alcohol extract of part of the traditional Chinese medicines is detected:

the test group was treated in an atmosphere of 1 + -0.5% oxygen volume concentration, 30 + -3 ℃ temperature and 50 + -10% humidity for 6 weeks, and the control group was stored in a cool-shade storehouse for 6 weeks. The results of the content of the dilute alcohol extract of the samples of the comparative test group and the control group after the completion of the test are shown in FIG. 6. FIG. 6 is a graph showing the change in the content of the dilute alcohol extract of a part of the sample before and after the test.

And (3) test results: as can be seen from FIG. 6, before and after modified atmosphere treatment, the detection results of the diluted alcohol extracts of 15 varieties are basically consistent and no obvious difference occurs, thus proving that the conditions (the oxygen volume concentration of 1 +/-0.5%, the controlled temperature of 30 +/-3 ℃ and the humidity of 50% +/-10%) set by the modified atmosphere parameters in the method for dynamic modified atmosphere disinsection of traditional Chinese medicinal materials by using the modified atmosphere storage do not influence the content of the traditional Chinese medicine extract.

(3) According to the test method, the influence of the method for performing the dynamic controlled atmosphere disinsection on the traditional Chinese medicine by using the controlled atmosphere storage on the component content change of part of the traditional Chinese medicine samples is detected:

the test group was treated in an atmosphere of 1 + -0.5% oxygen volume concentration, 30 + -3 ℃ temperature and 50 + -10% humidity for 6 weeks, and the control group was stored in a cool-shade storehouse for 6 weeks. The results of the content change of the samples of the control group and the control group after the completion of the test are shown in FIG. 7. FIG. 7 is a graph showing the change in the content of a part of the sample before and after the test.

And (3) test results: as can be seen from FIG. 7, the detection results of 12 index components of 9 varieties are basically consistent and no obvious difference occurs before and after the modified atmosphere treatment, which proves that the conditions (the oxygen volume concentration of 1 +/-0.5%, the controlled temperature of 30 +/-3 ℃ and the humidity of 50 +/-10%) set by the modified atmosphere parameters in the method for performing dynamic modified atmosphere disinsection on the traditional Chinese medicinal materials by using the modified atmosphere storage do not influence the content of the index components of the traditional Chinese medicines.

(4) The influence of modified atmosphere treatment on the appearance of part of Chinese medicinal materials (cinnamon, immature bitter orange, gardenia, mint, rehmannia root, ginseng, dried ginger and caulis sinomenii) according to the test method is as follows:

the test group was treated in an atmosphere of 1 + -0.5% oxygen volume concentration, 30 + -3 ℃ temperature and 50 + -10% humidity for 6 weeks, and the control group was stored in a cool-shade storehouse for 6 weeks. The results of appearance change of the control group and the control group after the completion of the test are shown in FIGS. 8 to 15.

And (3) test results: as can be seen from fig. 8-15, before and after the modified atmosphere treatment, the appearances of 8 traditional Chinese medicinal materials (cinnamon, immature bitter orange, gardenia, mint, rehmannia root, ginseng, dried ginger and caulis sinomenii) are not obviously different, and it is proved that the conditions (the oxygen volume concentration is 1 plus or minus 0.5%, the controlled temperature is 30 plus or minus 3 ℃, and the humidity is 50 plus or minus 10%) set by the modified atmosphere parameters in the method for performing dynamic modified atmosphere disinsection on the traditional Chinese medicinal materials by using the modified atmosphere storage according to the present invention do not affect the appearances of the traditional Chinese medicinal materials.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (1)

1. A method for performing dynamic controlled atmosphere disinsection on traditional Chinese medicinal materials by using a controlled atmosphere storage is characterized by comprising the following steps:

(1) putting the traditional Chinese medicinal materials to be treated into a warehouse, and sealing the warehouse;

(2) building a low-oxygen environment in the warehouse in the step (1), controlling the temperature and the humidity in the warehouse, performing controlled atmosphere treatment, and then controlling the time of the controlled atmosphere treatment, so that the purpose of performing dynamic controlled atmosphere disinsection on the traditional Chinese medicinal materials by using the controlled atmosphere warehouse can be realized;

in the step (2), the construction of the low-oxygen environment is realized by dynamically filling nitrogen or carbon dioxide; the volume concentration of the oxygen is 0.3 +/-0.2%; the temperature is 35 +/-1 ℃, and the humidity is 40 +/-10%; according to different types of the treated traditional Chinese medicines, the time of the modified atmosphere treatment is 2-4 weeks;

in the step (1), the traditional Chinese medicinal materials are placed in a warehouse in a stacking mode, and the warehouse is a sealed warehouse with air tightness meeting requirements;

and (3) after the air conditioning treatment in the step (2) is finished, opening the warehouse, ventilating for 20min, and after the oxygen volume concentration outside the warehouse is the same as the oxygen volume concentration in the warehouse, enabling workers and equipment to enter the warehouse and move out the traditional Chinese medicinal materials to perform subsequent production operation.

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