CN111812290B - Water pollution monitoring biosensor, monitoring system and monitoring method - Google Patents
Water pollution monitoring biosensor, monitoring system and monitoring method Download PDFInfo
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
- G01L1/00—Measuring force or stress, in general
- G01L1/24—Measuring force or stress, in general by measuring variations of optical properties of material when it is stressed, e.g. by photoelastic stress analysis using infrared, visible light, ultraviolet
- G01L1/242—Measuring force or stress, in general by measuring variations of optical properties of material when it is stressed, e.g. by photoelastic stress analysis using infrared, visible light, ultraviolet the material being an optical fibre
- G01L1/246—Measuring force or stress, in general by measuring variations of optical properties of material when it is stressed, e.g. by photoelastic stress analysis using infrared, visible light, ultraviolet the material being an optical fibre using integrated gratings, e.g. Bragg gratings
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a water pollution monitoring biosensor, a monitoring system and a monitoring method, wherein the monitoring biosensor comprises the following components: aquatic organisms, which are living bivalve organisms; the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms; the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure; the opening and closing of the two shells of the aquatic organisms drive the bending parts to expand or contract through the two fixing bases, and then the grating is driven to regularly expand or contract to generate signal change.
Description
Technical Field
The invention relates to the technical field of water pollution monitoring, in particular to a water pollution monitoring biosensor, a monitoring system and a monitoring method.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
The conventional water quality monitoring usually needs to be carried out on the spot at a water source, and sample water is brought back to a laboratory or a detection center for off-line detection.
At present, some electronic products can realize on-line water quality monitoring, but the electronic products need power supply, are difficult to be arranged at multiple points in a large-scale water area, are easy to be attached by aquatic organisms to lose efficacy particularly in seawater, and are difficult to work for a long time in a marine corrosion environment. The sensor is installed on an organism to realize environmental monitoring, but most of the sensors are realized by adopting an electronic sensor, and the sensor also has the problems of corrosion resistance, difficulty in power supply, easiness in signal transmission interference and the like, can not be used for long-term online monitoring, and is not beneficial to monitoring the water pollution condition.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention aims to provide a water pollution monitoring biosensor, a monitoring system and a monitoring method.
To solve the above technical problem, one or more of the following embodiments of the present invention provide the following technical solutions:
in a first aspect, the present invention provides a water pollution monitoring biosensor comprising:
aquatic organisms, which are living bivalve organisms;
the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms;
the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure;
the opening and closing of the two shells of the aquatic organisms drive the bending parts to expand or contract through the two fixing bases, and then the grating is driven to regularly expand or contract to generate signal change.
In a second aspect, the present invention provides a water pollution monitoring system comprising:
the water pollution monitoring biosensor;
the demodulator is connected with the grating of the biosensor through an optical cable;
and the computer is connected with the demodulator.
In a third aspect, the present invention provides a water pollution monitoring method, comprising the steps of:
the breathing of the bivalve shellfish organism causes the opening and closing movement of the two shells, and the opening and closing movement is transmitted to the elasticity Liang Pianshang through the two fixed bases to cause the relaxation or the compression of the bending part of the elastic beam piece, so that the grating tightly attached to the bending part of the elastic beam piece is synchronously deformed to cause the change of the central wavelength of the grating;
the water quality condition of the water area where the bivalve shellfish organism is located is monitored by monitoring the change rule of the central wavelength of the grating.
Compared with the prior art, one or more technical schemes of the invention have the following beneficial effects:
when the water pollution monitoring biosensor is used, the first fixing base and the second fixing base are fixed on bivalve shellfish organisms such as scallops and the like, then the bivalve shellfish organisms are placed in a water body to be monitored, and a series of indexes such as water pollution, oxygen content, pesticide content and the like are reflected in real time through monitoring the change of physiological characteristics of the organisms to complete the monitoring of water pollution, so that the monitoring of the water pollution condition by means of a biological carrier is realized, and the water body monitoring is convenient.
The fiber Bragg grating is attached to the outer side of the bending part, so that uniform strain is easy to occur, and a regular signal is generated. In addition, the bending part has certain elasticity, can reset in time, and plastic deformation can not appear in certain stress range to guarantee the accuracy of monitoring.
The water pollution monitoring method utilizes bivalve shellfish organisms as a perceptron of water pollution degree, and obtains the activity characteristics of the organisms through the acquisition of the optical fiber sensor, thereby realizing the monitoring of water pollution indexes, and having more effective and direct online monitoring effects on water pollution monitoring, in particular on water pollution monitoring which can directly influence the organisms.
The optical fiber Bragg grating is used for sensing the activity characteristics of the bivalve shellfish organism, strain signals of the grating are transmitted outwards through the optical cable, and when monitoring is carried out in the mode, the monitoring distance can reach hundreds of kilometers, a relay station is not needed, power supply is not needed, the problems that the conventional biosensor is difficult to carry out offline sampling and long-term remote online monitoring of an electronic sensor are solved, and a necessary monitoring means is provided for laying of an ocean information monitoring network.
In addition, a plurality of biosensors can be connected in series, the series detection of the biosensors can be realized by adopting wavelength division multiplexing, the wiring and branching difficulty is greatly reduced, and convenience is provided for the actual ocean layout.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a schematic structural diagram of a water pollution monitoring biosensor provided in an embodiment of the present invention;
FIG. 2 is a schematic view of a partial structure of a water pollution monitoring biosensor provided in an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of an elastic beam piece according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a first fixing base according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a second fixing base according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a water pollution monitoring system according to an embodiment of the present invention;
FIG. 7 is a diagram illustrating examples of the health and disease waveforms of bivalve organisms according to an embodiment of the present invention;
fig. 8 is a waveform diagram of respiration, filter feeding and dormancy of bivalve shellfish organisms under a normal water quality environment according to an embodiment of the invention.
The device comprises a base, a first fixing base, a first clamping groove, a first hole and a second clamping groove, wherein 1, the first fixing base 101, the first clamping groove 102 and the first hole are arranged;
2. a second fixed base 201, a second card slot 202, a second hole 203, and a fiber slot;
3. an elastic beam piece 301, a first fixing part 302, a second fixing part 303 and a bending part;
4. the device comprises a fiber Bragg grating 5, an isolation cabin 6, an optical cable 7, an optical cable buffer protective sleeve 8, a computer 9, a demodulator 10 and a bivalve shellfish.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
In a first aspect, the present invention provides a water pollution monitoring biosensor comprising:
aquatic organisms, which are living bivalve organisms;
the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms;
the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure;
the opening and closing of the two shells of the aquatic organisms drive the bending parts to expand or contract through the two fixing bases, and then the grating is driven to regularly expand or contract to generate signal change.
In some embodiments, when the bending portion is not elastically deformed, the first fixing base is parallel to the second fixing base. By adopting the structure, the opening and closing between the two fixed bases can be more sensitively transmitted to the bending part, so that the deformation of the bending part is more sensitive, and the monitoring sensitivity is favorably improved.
In some embodiments, the outer sidewall of the bend is within an isolation chamber, the isolation chamber being filled with an isolation fluid.
The outer side wall of the bending part is the side wall of the convex side of the bending part, the grating is pasted on the side wall, the grating is placed in the isolation liquid, the grating can be isolated from the external water body, the normal work of the grating is guaranteed, and the service life of the grating is guaranteed.
Furthermore, the waterproof partition wall is made of rubber or plastic.
Further, the isolation liquid is silicone oil. The silicone oil has high heat resistance, water resistance, electric insulation and small surface tension, and can be used as a release agent. Other liquid components can also need to have the characteristics of no corrosion to metal and rubber, no icing, no vaporization, small expansion coefficient and the like in the temperature range of the conventional seawater or fresh water in four seasons.
Furthermore, the isolation bin is wrapped outside the elastic beam piece and the grating.
Furthermore, one end of the isolation bin is fixed on the first fixed base, and the other end of the isolation bin is fixed on the second fixed base.
In some embodiments, the first fixing base is provided with a first clamping groove, the second fixing base is provided with a second clamping groove, and the first fixing portion and the second fixing portion of the elastic beam piece are inserted into the first clamping groove and the second clamping groove respectively.
Furthermore, the elastic beam piece is made of stainless steel or fiber composite materials. Has strong elasticity and seawater erosion resistance.
In some embodiments, the first and second fixed bases are bonded to the shell of the bivalve shellfish organism by a waterproof glue.
Furthermore, a first hole is formed in the bonding position of the first fixing base and the shell, a second hole is formed in the bonding position of the second fixing base and the shell, and the first hole and the second hole are through holes.
The arrangement of the first hole and the second hole facilitates waterproof glue coating between the fixed base and the shell, so that the bonding strength between the fixed base and the shell is improved. In addition, adopt this kind of structure, can be earlier with fixed baseplate and casing location back, the inside injection adhesive in rethread first hole and second hole helps realizing fixed baseplate's location better and fixes.
In some embodiments, a groove is disposed on the first fixing base or the second fixing base.
The groove body is used for placing an optical cable so as to connect the optical cable with the grating.
In a second aspect, the present invention provides a water pollution monitoring system comprising:
the water pollution monitoring biosensor;
the demodulator is connected with the grating of the biosensor through an optical cable;
and the computer is connected with the demodulator.
In some embodiments, a cable buffer protective jacket is disposed at the junction of the grating and the cable. The optical fiber Bragg grating protection device is used for protecting the connection of the optical fiber Bragg grating and the optical cable.
Further, the optical cable buffer protective sleeve is arranged in the optical fiber groove.
The quantity of the water pollution monitoring biosensors can be expanded according to actual needs, and the biosensors are connected in series.
In a third aspect, the present invention provides a water pollution monitoring method, comprising the steps of:
the breathing of the bivalve shellfish organism causes the opening and closing movement of the two shells, and the opening and closing movement is transmitted to the elasticity Liang Pianshang through the two fixed bases to cause the relaxation or the compression of the bending part of the elastic beam piece, so that the grating tightly attached to the bending part of the elastic beam piece is synchronously deformed to cause the change of the central wavelength of the grating;
the water quality condition of the water area where the bivalve shellfish organism is located is monitored by monitoring the change rule of the central wavelength of the grating.
The embodiment of the invention provides a novel water pollution monitoring biosensor which is used for being fixed on bivalve shellfish organisms such as scallops. Fig. 1 is a schematic structural diagram of a water pollution monitoring sensor according to an embodiment of the present invention. As shown in fig. 1, a water pollution monitoring sensor provided in an embodiment of the present invention includes a first fixed base 1, a second fixed base 2, an elastic beam piece 3, and a fiber bragg grating 4.
The one end fixed connection first fixed baseplate 1 of elastic beam piece 3, the other end fixed connection second fixed baseplate 2 of elastic beam piece 3, and elastic beam piece 3 realizes linking together first fixed baseplate 1 and second fixed baseplate 2 promptly. The first fixing base 1 and the second fixing base 2 are used for fixedly connecting a shell of a bivalve shellfish organism such as a scallop. The elastic beam piece 3 has good elasticity and can play a good role in transmission. In the embodiment of the invention, the rear ends of the first fixed base 1 and the second fixed base 2 are connected with the elastic beam pieces 3, and the front ends of the first fixed base 1 and the second fixed base 2 are used for fixedly connecting bivalve shellfish organisms such as scallops.
When fixing water pollution monitoring sensor on the organism through first fixed baseplate 1 and second fixed baseplate 2, the breathing of organism causes opening and shutting of two upper and lower shells to drive the opening and shutting motion of first fixed baseplate 1 and second fixed baseplate 2, the opening and shutting motion of first fixed baseplate 1 and second fixed baseplate 2 is conducted to elastic beam piece 3, causes the bending deformation of elastic beam piece 3. The fiber Bragg grating 4 is fixedly arranged on the elastic beam piece 3, when the elastic beam piece 3 is bent and deformed, the bending deformation can cause the fiber Bragg grating 4 tightly attached to the elastic beam piece 3 to synchronously generate strain, so that the central wavelength of the fiber Bragg grating 4 is changed.
The central reflection wavelength of the fiber bragg grating is:
(1) In the formula: />Is the effective refractive index of the guided mode>Is the natural period of the grating.
When the wavelength satisfies the bragg condition (1), the incident light will be reflected back by the fiber grating.
As can be seen from the formula (1), the center reflection wavelength of the fiber gratingFollow and/or answer>And &>Is changed. The fiber Bragg grating is sensitive to stress, which is determined by the elasto-optical effect and the fiber Bragg grating period->To influence->。
When the fiber grating is only stressed, the refractive index and period of the fiber grating change, causing the central reflection wavelengthMoving, therefore, there are:
Refractive index change when the grating is stressed:
in the formula:
is an axial stress, is greater than or equal to>Is the poisson's ratio of the core material, device for combining or screening>、/>Is a light-flicking factor, based on the comparison result>Is the effective elasto-optic coefficient.
The fiber grating is assumed to be absolutely uniform, that is, the relative rate of change of the period of the grating and the relative rate of change of the physical length of the grating segments are uniform.
So equation (3) can be written as:
equation (6) is a general calculation equation for bare fiber grating stress measurement.
Axial stressIs caused by the bending of the beam piece caused by the opening and closing of the bivalve shellfish organism suffered by the grating, namely the stress change of the fiber grating caused by the opening and closing of the bivalve shellfish organism, the breathing of the bivalve shellfish and the opening and closing degree of the filter-eating shell can be established through the formula (6)QAnd the central wavelength of the fiber grating>The corresponding relation of (1):
According to the formula, the frequency characteristic rule of opening and closing breath and filter food of the bivalve shellfish can be measured only by measuring the variable quantity of the central wavelength of the grating.
In the embodiment of the invention, the bivalve shellfish organism is preferably a scallop, which has strong viability but is not limited to the scallop and can be selected according to the actual detection water body. When the scallop is selected, as shown in the attached drawing 1, the bivalve shellfish 10 is clamped at one end of the first fixed base 1 and the second fixed base 2 far away from the end fixedly connected with the elastic beam piece 3, and the first fixed base 1 and the second fixed base 2 are respectively and fixedly connected with the upper shell and the lower shell of the bivalve shellfish 10.
The corresponding relation between the breathing and filtering rule of the organism and the water quality components can establish a professional database according to different components and organism characteristic change rules, the response relations of different shells to different components are different, the response relations of the same shell to different components are different, however, the corresponding response relations of the determined shell and the determined components are determined, for example, obvious corresponding relation exists between petroleum pollution and the breathing activity of the scallop opening and closing. Therefore, the water pollution is monitored by monitoring the physiological characteristic change of the organism and reflecting a series of indexes such as water pollution, oxygen content, pesticide content and the like in real time, the water pollution condition is monitored by means of a biological carrier, and the water body is conveniently monitored.
The water pollution monitoring sensor provided by the embodiment of the invention is mainly used in water body environments, such as ocean water bodies and the like. In order to prevent the water from corroding the elastic beam pieces 3 and the fiber bragg grating 4 in the water or being attached by organisms in the water to influence the use of the water pollution monitoring sensor, the water pollution monitoring sensor provided by the embodiment of the invention further comprises an isolation bin 5. The isolation bin 5 is fixedly connected with the first fixing base 1 and the second fixing base 2, the elastic beam piece 3 and the fiber Bragg grating 4 are located in the isolation bin 5, and the isolation bin 5 is used for preventing the elastic beam piece 3 and the fiber Bragg grating 4 from being corroded in a water body or being attached to the water body by organisms. The isolation bin 5 is made of an elastic soft material. Optionally, the elastic soft material of the isolation bin 5 can be made of waterproof rubber material or waterproof film material. Furthermore, the isolation bin 5 is filled with silicone oil, so that the elastic beam piece 3 and the fiber Bragg grating 4 can be better protected, and the water pollution monitoring sensor can be conveniently used underwater.
The elastic beam piece 3 can be made of high Cr, ni, low C seawater corrosion resistant stainless steel, feAlMo alloy stainless steel material, elastic seawater corrosion resistant fiber composite material and the like, so that the elastic beam piece 3 has stronger elasticity and seawater corrosion resistance.
The isolation bin 5 comprises an isolation bin inner side sheet and an isolation bin outer side sheet, one end of the isolation bin inner side sheet and one end of the isolation bin outer side sheet are fixedly connected with the first fixing base 1, the other end of the isolation bin inner side sheet and the other end of the isolation bin outer side sheet are fixedly connected with the second fixing base 2, the isolation bin outer side sheet is located at the end portions of the first fixing base 1 and the second fixing base 2, the isolation bin inner side sheet is located on the inner side, opposite to the first fixing base 1 and the second fixing base 2, of the isolation bin inner side sheet and the isolation bin outer side sheet are connected to form a cavity, and the elastic beam sheet 3 and the fiber Bragg grating 4 are wrapped inside the isolation bin inner side sheet and the isolation bin outer side sheet.
Fig. 2 is a schematic partial structural diagram of a water pollution monitoring sensor according to an embodiment of the present invention. Fig. 2 shows the basic structure of the elastic beam piece 3, the first fixed base 1 and the second fixed base 2 in the embodiment of the present invention. FIG. 3 is a schematic structural diagram of an elastic beam piece provided in an embodiment of the present invention; fig. 4 is a schematic structural diagram of a first fixing base provided in the embodiment of the present invention; fig. 5 is a schematic structural diagram of a second fixing base provided in the embodiment of the present invention. The elastic beam piece 3, the first fixed base 1 and the second fixed base 2 provided by the embodiment of the present invention will be described in detail with reference to fig. 2 to 5.
As shown in fig. 3, the elastic beam piece 3 according to the embodiment of the present invention includes a first fixing portion 301, a second fixing portion 302, and a bending portion 303, and the bending portion 303 connects the first fixing portion 301 and the second fixing portion 302. The elastic beam piece 3 is fixedly connected with the first fixing base 1 through the first fixing part 301, the elastic beam piece 3 is fixedly connected with the second fixing base 2 through the second fixing part 302, and the fiber bragg grating 4 is fixedly arranged on the bending part 303. The curved portion 303 has a concave surface facing the first card slot 101 and the second card slot 201 and a convex surface facing the directions of 102 and 202, and the curved portion 303 is fixedly connected with 101 of the first fixing base 1 and 201 of the second fixing base 2 through 301 and 302.
Specifically, the first fixing portion 301 and the second fixing portion 302 are straight structures, which facilitate connection with the first fixing base 1 and the second fixing base 2, and a distance between the first fixing portion 301 and the second fixing portion 302 can be selected according to an actual thickness of a bivalve shellfish organism such as a scallop. In the embodiment of the present invention, the fiber bragg grating 4 is fixed on the bending portion 303, which facilitates the triggering of the fiber bragg grating 4. When the shells of the bivalve shellfish organisms such as scallops open and close to drive the first fixing base 1 and the second fixing base 2 to open and close, the first fixing base 1 and the second fixing base 2 which are opened and closed are transmitted to the bending part 303 of the elastic beam piece 3 through the first fixing part 301 and the second fixing part 302 of the elastic beam piece 3, so that the fiber bragg grating 4 is triggered, namely when the bending part 303 is subjected to bending deformation, the bending deformation can cause the fiber bragg grating 4 tightly attached to the elastic beam piece 3 to synchronously generate strain along with the fiber bragg grating, and the central wavelength of the fiber bragg grating 4 is changed. The combination of the first fixing part 301, the second fixing part 302 and the bending part 303 facilitates more accurate triggering of the fiber bragg grating 4, and further, the frequency characteristic rule of organism opening and closing respiration and filter feeding can be more accurately measured.
Preferably, the fiber bragg grating 4 is disposed outside the bending portion 303 of the elastic beam piece 3, which is beneficial to triggering the fiber bragg grating 4 and is convenient for installing the fiber bragg grating 4.
As shown in fig. 4, in the embodiment of the present invention, a first card slot 101 is provided on the first fixing base 1. The elastic beam piece 3 is connected with the first fixed base 1 through a first clamping groove 101 in a clamping mode. The first fixing portion 301 of the elastic beam piece 3 is engaged with the first engaging groove 101.
As shown in fig. 4, a first hole 102 is provided at an end of the first fixed base 1 remote from the end to which the elastic beam piece 3 is fixedly connected. In the embodiment of the invention, the first fixing base 1 is usually fixed on the shells of the bivalve shellfish organism such as scallop by waterproof glue, so the arrangement of the first hole 102 is convenient for spreading the waterproof glue, the connection between the shells and the water pollution monitoring sensor is reinforced, the water pollution monitoring sensor is effectively prevented from falling off from the shells, and the stability of the connection between the water pollution monitoring sensor and the shells is improved. In the embodiment of the present invention, the first hole 102 is a through hole. Preferably, the number of the first holes 102 is two, but not limited to two, and can be selected according to specific use requirements.
Further, as shown in fig. 5, in the embodiment of the present invention, a second card slot 201 is disposed on the second fixing base 2. The elastic beam piece 3 is connected with the second fixed base 2 through a second clamping groove 201 in a clamping mode. Optionally, the second fixing portion 302 of the elastic beam piece 3 is connected to the second slot 201 in a snap-fit manner.
As shown in fig. 5, a second hole 202 is provided at an end of the second fixed base 2 remote from the end to which the elastic beam piece 3 is fixedly connected. In the embodiment of the invention, the water pollution monitoring sensor usually adopts waterproof glue to fix the second fixing base 2 on the shells of the bivalve shellfish organisms such as scallops, so the second hole 202 is arranged to facilitate the waterproof glue spreading, reinforce the connection between the shells and the water pollution monitoring sensor, effectively prevent the water pollution monitoring sensor from falling off the shells, and improve the stability of the connection between the water pollution monitoring sensor and the shells. In the present embodiment, the second hole 202 is a through hole. Preferably, the number of the second holes 202 is two, but not limited to two, and can be selected according to specific use requirements.
Furthermore, in order to conveniently realize the fixed connection between the first fixing base 1 and the second fixing base 2 and the bivalve shellfish organisms such as scallops, the front ends of the first fixing base 1 and the second fixing base 2 are provided with through holes 102 and 202 for effectively fixing the shells and the bases and preventing the shells and the bases from sliding and loosening.
In the embodiment of the invention, the water pollution monitoring sensor further comprises an optical cable 6, and the optical cable 6 is connected with the fiber bragg grating 4. The optical cable 6 is used for outputting the central wavelength of the fiber Bragg grating 4, so that the connection of the water pollution monitoring sensor is more convenient.
Further, an optical cable buffering protective sleeve 7 is arranged at the joint of the fiber bragg grating 4 and the optical cable 6, and the optical cable buffering protective sleeve 7 is sleeved on the joint of the fiber bragg grating 4 and the optical cable 6. The cable buffer protective sheath 7 is used for protecting the connection of the fiber bragg grating 4 and the optical cable 6.
Furthermore, in order to facilitate the installation of the optical cable 6, an optical fiber groove is arranged on the first fixing base 1 or the second fixing base 2, and the optical cable buffer protective sleeve 7 is embedded in the optical fiber groove. As shown in fig. 5, the second fixing base 2 is provided with an optical fiber groove 203, the optical fiber groove 203 passes through the second clamping groove 201, the optical cable buffering protective sleeve 7 is embedded in the optical fiber groove 203, and after the second clamping groove 201 clamps the elastic beam piece 3, the optical cable 6 is pressed in the optical fiber groove 203 through the optical cable buffering protective sleeve 7.
Based on the water pollution monitoring sensor provided by the embodiment of the invention, the invention also provides a water pollution monitoring system. Fig. 6 is a schematic structural diagram of a water pollution monitoring system provided in an embodiment of the present invention. As shown in fig. 6, the water pollution monitoring system provided in the embodiment of the present invention includes a computer 8, a demodulator 9, and a plurality of water pollution monitoring sensors. The water pollution monitoring sensor is the water pollution monitoring sensor in the embodiment. The signal input end of the demodulator 9 is connected with the output end of the fiber bragg grating 4 of the water pollution monitoring sensor through an optical cable, and the signal output end of the demodulator 9 is connected with the computer 8. The computer 8 obtains the change curve of the grating center wavelength of the fiber Bragg grating 4 through the demodulator 9, so that the breathing and filter eating characteristic rule of the organism can be obtained, and the corresponding water pollution characteristic parameter can be further calculated.
In the embodiment of the invention, a plurality of water pollution monitoring sensors can be installed in the water pollution monitoring system, and the number of the sensors can be expanded according to actual needs. Preferably, the fiber grating reference center wavelengths adopted by each water pollution monitoring sensor are different, so that wavelength division multiplexing can be realized, that is, a group of water pollution monitoring sensors including a plurality of sensing probes can share one optical fiber/optical cable channel without crosstalk, and a plurality of water pollution monitoring sensors can be connected in parallel through an optical fiber splitter or an optical fiber coupler or can be designed to be in a series connection mode. And each group of water pollution monitoring sensors are connected through optical cables to carry out signal transmission. The optical fiber cable is a tensile multi-core optical cable such as Teflon and the like, and can be used for signal transmission and can also be used as a cable for retracting and releasing the sensor.
In the water pollution monitoring system provided by the embodiment of the invention, the bivalve shellfish organisms which are healthy in life and suitable for the existence of the monitored water area, such as scallops, are selected, the water pollution monitoring sensor is assembled and packaged according to the structure shown in fig. 6, the bivalve shellfish organisms are stably clamped between the first fixing base 1 and the second fixing base 2 of the water pollution monitoring sensor, and the fast curing cement or water-resistant glue is coated through the first holes and the second holes reserved on the upper base and the lower base, so that the bivalve shellfish organisms can be firmly fixed on the first fixing base 1 and the second fixing base 2 for a long time.
The demodulator 9 is connected with the water pollution monitoring sensor array through the connecting optical fiber cable 6, the demodulator 9 is connected with the computer 8 through a network cable interface, and data reading software is arranged on the computer and can monitor the central wavelength of the optical fiber Bragg grating packaged in each water pollution monitoring sensor in real time.
For example, fig. 7 is a waveform diagram of the health and disease states of bivalve organisms before and after water poisoning, and fig. 8 is a waveform diagram of respiration, filter feeding and dormancy of a certain bivalve organism in a normal water environment. Then the water quality can be monitored in real time according to the data in fig. 7 and 8. For example, some mussel shells are open for 70-80% of the time to ingest food and oxygen, but in the event of water contamination, the time for their shells to close is altered.
Meanwhile, different organism species have different basic biological characteristics, so that a plurality of water pollution monitoring sensors are often arranged in an array manner in practical application, and the adopted organism species are ensured to be consistent. In addition, a standard water tank is arranged in the water area, the water quality in the water tank is standard healthy water quality, for example, the seawater is a pollution-free healthy seawater source, the fresh water is taken from a common drinking water source, and the like, and common water without obvious pollution is obtained. Data collected by water pollution monitoring sensors distributed in the water tank are set as standard value signals, signals of other sensors are compared with the standard value signals for observation, if the characteristics are the same or similar, the water quality is considered to be safe, and if the characteristics are large fluctuation differences, the water quality is judged to be abnormal. The water pollution monitoring sensors distributed at the same water area are mutually referenced, and when one water pollution monitoring sensor is abnormal, for example, when signal characteristics disappear due to death of organisms, and other water pollution monitoring sensors are not abnormal, abnormal water pollution monitoring sensor signals can be eliminated and treated as failure signals.
In the embodiment of the invention, a plurality of water pollution monitoring sensors are connected in series in the water pollution monitoring system. The series connection mode can adopt one optical fiber to string a plurality of water pollution monitoring sensors, when a plurality of sensors are required to be arranged, only one optical cable is connected in series and then wavelength division multiplexing is adopted, so that wiring and branching difficulty is greatly reduced, convenience is provided for arrangement of water pollution monitoring, and particularly convenience is provided for actual ocean arrangement.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (13)
1. A water pollution monitoring biosensor, comprising: the method comprises the following steps:
aquatic organisms, which are living bivalve organisms;
the grating fixing structure comprises a first fixing base, a second fixing base and an elastic beam piece, wherein the elastic beam piece is of an arch structure, and a first fixing part and a second fixing part of the elastic beam piece are respectively arranged on the first fixing base and the second fixing base, so that a bending part in the middle of the elastic beam piece is positioned between the two fixing bases; the two fixed bases are respectively fixed on the surfaces of the two shells of the aquatic organisms;
the grating is a fiber Bragg grating and is adhered to the outer side of the bending part of the grating fixing structure;
the outer side wall of the bending part is positioned in the isolation bin, the isolation bin is formed by surrounding the bending part and a waterproof isolation wall, and isolation liquid is filled in the isolation bin;
the waterproof isolation wall is made of rubber or plastic;
the isolation liquid is corrosion-resistant, low in expansion coefficient and not easy to freeze or vaporize;
the isolation bin is wrapped outside the elastic beam piece and the grating;
one end of the isolation bin is fixed on the first fixed base, and the other end of the isolation bin is fixed on the second fixed base.
2. The water pollution monitoring biosensor as recited in claim 1, wherein: when the bending part is not elastically deformed, the first fixed base is parallel to the second fixed base.
3. The water pollution monitoring biosensor as recited in claim 1, wherein: the first fixing base is provided with a first clamping groove, the second fixing base is provided with a second clamping groove, and the first fixing part and the second fixing part of the elastic beam piece are inserted into the first clamping groove and the second clamping groove respectively.
4. The water pollution monitoring biosensor as recited in claim 3, wherein:
the elastic beam piece is made of stainless steel or fiber composite materials.
5. The water pollution monitoring biosensor as recited in claim 1, wherein: the first fixing base and the second fixing base are bonded on the shell of the bivalve shellfish organism through waterproof glue.
6. The water pollution monitoring biosensor as recited in claim 5, wherein: the first fixing base is provided with a first hole at the bonding position with the shell, the second fixing base is provided with a second hole at the bonding position with the shell, and the first hole and the second hole are through holes.
7. The water pollution monitoring biosensor as recited in claim 1, wherein: a groove body is arranged on the first fixing base or the second fixing base.
8. A water pollution monitoring system, characterized by: the method comprises the following steps:
a water pollution monitoring biosensor as claimed in claim 1;
the demodulator is connected with the grating of the biosensor through an optical cable;
the computer is connected with the demodulator;
a plurality of biosensors are connected in series, and the series monitoring of the biosensors is realized by adopting wavelength division multiplexing.
9. The water pollution monitoring system according to claim 8, wherein: and an optical cable buffer protective sleeve is arranged at the joint of the grating and the optical cable.
10. The water pollution monitoring system according to claim 9, wherein: the optical cable buffer protective sleeve is arranged in the optical fiber groove.
11. The water pollution monitoring system according to claim 9, wherein: the reference center wavelength of the grating used by each sensor is different.
12. The water pollution monitoring system according to claim 8, wherein: the water pollution monitoring device further comprises a standard water tank, and standard water and a water pollution monitoring biosensor are placed in the standard water tank.
13. A method for monitoring water pollution using the water pollution monitoring biosensor of claim 1, wherein: the method comprises the following steps:
the opening and closing movement of the two shells caused by the respiration of the bivalve shellfish organism is transmitted to the elasticity Liang Pianshang through the two fixed bases to cause the relaxation or the compression of the bending part of the elastic beam piece, so that the grating tightly attached to the bending part of the elastic beam piece is synchronously deformed to cause the change of the central wavelength of the grating;
the water quality condition of the water area where the bivalve shellfish organism is located is monitored by monitoring the change rule of the central wavelength of the grating.
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