CN111808719A

CN111808719A - Microbial bacterium culture device for improving culture environment

Info

Publication number: CN111808719A
Application number: CN202010721732.5A
Authority: CN
Inventors: 路庆鹏; 金雪霞
Original assignee: JIANGSU LVKE BIOTECHNOLOGY CO Ltd
Current assignee: JIANGSU LVKE BIOTECHNOLOGY CO Ltd
Priority date: 2020-07-24
Filing date: 2020-07-24
Publication date: 2020-10-23
Anticipated expiration: 2040-07-24
Also published as: CN111808719B

Abstract

The invention relates to the technical field of microbial cultivation, in particular to a microbial cultivation device for improving a cultivation environment, which comprises an incubator, wherein a control box is installed at the lower end of the incubator, supporting legs are welded on the lower surface of the control box, a cabinet door is rotatably installed on the front surface of the incubator, an exhaust net is installed at the upper end of the outer surface of one side of the incubator, a mother material box is installed on the upper surface of the incubator, a discharge pipe is welded on the lower surface of the mother material box in a penetrating manner, an atomization box is installed on the upper surface of the incubator, which faces to a position close to the mother material box, and an air outlet pipe is welded on the upper surface of. The invention is a centrifugal mixed material, a flow sensor is arranged at a master batch outlet, the device works in a closed mode, the interior of the device is heated and humidified in the culture process, the cultured material is convenient to take out, three groups of culture vessels are arranged, and the material culture in the three vessels can be used for carrying out a comparison experiment.

Description

Microbial bacterium culture device for improving culture environment

Technical Field

The invention relates to the technical field of microbial culture, in particular to a microbial culture device for improving a culture environment.

Background

The process of inoculating a microorganism in a laboratory onto an artificial medium suitable for its growth and reproduction or into a living organism is called inoculation, and includes liquid inoculation, puncture inoculation, live inoculation, mixed inoculation, streaking inoculation, spread inoculation, three-point inoculation, injection inoculation, and fermentation culture in a greenhouse environment after inoculation to increase the number of bacteria.

However, microbial cultivation device on the existing market, generally be manual mixing, carry out the interpolation of fungus mother, the quantity that the interpolation process added can not obtain the control, be unfavorable for the success rate that the microbial cultivation was cultivateed, the material after most vaccinations passes through the puddler stirring, the stirring is inhomogeneous, and the material adheres to on the puddler easily, be unfavorable for the cleanness of device, the condition of cultivateing of the inside material of viewing device that can not audio-visual mostly, be unfavorable for the control to the material, the material after the cultivation generally takes out inconveniently, be unfavorable for taking out, cultivate successful material, and generally only have a culture ware, it summarizes all extravagantly to lead to once vaccinate all materials unsuccessfully, and cultivate the use that can postpone continuation microbial fungus once more again, be unfavorable for the farming environment in time to obtain the improvement, and can not carry. Accordingly, one skilled in the art provides a microbial cultivation apparatus for cultivation environment improvement to solve the above problems in the background art.

Disclosure of Invention

The present invention is directed to a microbial cultivation apparatus for improving cultivation environment, which solves the above problems of the prior art.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a microbial cultivation device for aquaculture environment improvement, includes the incubator, the control box is installed to the lower extreme of incubator, the lower skin weld of control box has the supporting legs, the front surface of incubator rotates installs the cabinet door, the exhaust net is installed to one side surface upper end of incubator, the last surface mounting of incubator has the mother's workbin, the lower surface through-welding of mother's workbin has the discharging pipe, the last surface of incubator faces the position department of nearly mother's workbin and installs the atomizing case, the upper surface through-welding of atomizing case has the outlet duct, the ware seat is installed to the inside lower extreme of incubator, the internally mounted of ware seat has household utensils, temperature and humidity sensor is installed to inside one side of incubator, the inside lower surface mounting of incubator has the heating band.

As a further scheme of the invention: the lower surface welding of utensil seat has the bull stick, the inside lower surface mounting of incubator has the motor, the surface mounting of bull stick has the drive belt, the singlechip is installed to inside lower surface one side of control box, the display screen is installed to front surface one side of control box, the control button is installed in the external department that the front surface of control box closes on the display screen.

As a still further scheme of the invention: the discharging electromagnetic valve is installed to the lower extreme of discharging pipe, the external department that the lower extreme of discharging pipe closes on discharging electromagnetic valve installs the flow valve, the solenoid valve of giving vent to anger is installed to the upper end of outlet duct, the steam trap is installed to the position department that the upper end of outlet duct closes on the atomizer box, the internally mounted of atomizer box has the atomizing piece.

As a still further scheme of the invention: the other end of the air outlet pipe penetrates through the upper surface of the incubator and is inserted into the inner position of the incubator, three groups of ware seats and three groups of ware are arranged on the ware seat, the ware seat and the ware are both rotatably installed at the inner position of the incubator, the lower end of the discharge pipe is provided with three discharge branch pipes, and the discharge branch pipes are in one-to-one correspondence with the positions of the three ware seats.

As a still further scheme of the invention: the incubator is a stainless steel component, a through window is arranged on the cabinet door, and liquid level through windows are arranged on the front surfaces of the atomization box and the mother material box.

As a still further scheme of the invention: the output end electrode of the single chip microcomputer is connected with the display screen, the control key, the motor, the temperature and humidity sensor and the input end of the heating belt, and the output end electrode of the single chip microcomputer is connected with the discharge pipe, the gas outlet pipe and the input end of the electrode component on the atomization box.

As a still further scheme of the invention: the culture incubator is characterized in that a vessel seat is fixed above the motor, two other vessel seats are correspondingly arranged on two sides of the upper portion of the motor, the vessel seats on the two sides of the upper portion of the motor are rotatably installed at the inner position of the culture incubator through a rotating rod, and the rotating rod is rotatably connected with an output shaft of the motor through a transmission belt.

As a still further scheme of the invention: the three branch pipe departments of lower extreme of discharging pipe all install ejection of compact solenoid valve and flow valve, and the three position of branch pipe of lower extreme of discharging pipe respectively with the top of household utensils corresponding, the atomizer box passes through steam trap and outlet duct through connection.

Compared with the prior art, the invention has the beneficial effects that: the microbial bacteria culture device is a centrifugal mixed material, the base material and the master batch are uniformly mixed, the problems that the stirring is not uniform and the material is adhered to the stirring rod due to the stirring of the stirring rod are avoided, the subsequent cleaning work of the device is facilitated, a flow sensor is arranged at the position of the outlet of the master batch, the opening and closing of the electromagnetic valve are realized, the adding amount in the adding process can be accurately controlled, the success rate of microbial bacteria culture is improved, a through window is arranged in the device, the culture condition of the material in the device can be visually observed, the device works in a closed mode, the interior of the device is easily heated and humidified in the culture process, the culture environment of the material is optimized, the material is cultured by a test tube type culture vessel, the cultured material is conveniently taken out, three groups of culture vessels are arranged, the total loss of the material due to misoperation is avoided, and when the material, the breeding environment can be improved in time, and the material culture in the three vessels can be compared and tested, so that the operation is optimized, and the error rate is reduced.

Drawings

FIG. 1 is a schematic structural diagram of a microbial cultivation device for improving cultivation environment;

FIG. 2 is a perspective view of an incubator used in a microbial cultivation apparatus for improvement of cultivation environment;

FIG. 3 is a perspective partial view of an incubator of a microbial cultivation apparatus for cultivation environment improvement;

FIG. 4 is a perspective partial view of a microbial culture device incubator for culture environment modification;

FIG. 5 is a perspective view of an atomization box of a microbial cultivation apparatus for improvement of cultivation environment.

In the figure: 1. an incubator; 2. a control box; 3. supporting legs; 4. a cabinet door; 5. an atomization box; 6. a mother material box; 7. a vessel seat; 8. a vessel; 9. a temperature and humidity sensor; 10. an exhaust net; 11. a discharge pipe; 12. an air outlet pipe; 13. heating the tape; 14. a rotating rod; 15. a motor; 16. a transmission belt; 17. a display screen; 18. a control key; 19. a single chip microcomputer; 20. a discharge electromagnetic valve; 21. a flow valve; 22. a steam trap; 23. an air outlet electromagnetic valve; 24. atomizing sheet.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 to 5, in the embodiment of the invention, a microbial cultivation device for improving a cultivation environment comprises an incubator 1, a control box 2 is installed at the lower end of the incubator 1, supporting legs 3 are welded on the lower surface of the control box 2, a cabinet door 4 is rotatably installed on the front surface of the incubator 1, an exhaust net 10 is installed at the upper end of the outer surface of one side of the incubator 1, a mother material box 6 is installed on the upper surface of the incubator 1, a discharge pipe 11 is welded on the lower surface of the mother material box 6 in a penetrating manner, an atomization box 5 is installed at the position, close to the mother material box 6, of the upper surface of the incubator 1, an air outlet pipe 12 is welded on the upper surface of the atomization box 5 in a penetrating manner, a vessel seat 7 is installed at the lower end inside the incubator 1, a vessel 8 is installed inside the vessel seat 7, a temperature and humidity sensor 9 is, the other end of the air outlet pipe 12 penetrates through the upper surface of the incubator 1 and is inserted into the inner position of the incubator 1, three groups of vessel seats 7 and vessels 8 are arranged on the vessel seats 7, the three groups of vessel seats 7 and vessels 8 are rotatably arranged at the inner position of the incubator 1, the lower end of the discharge pipe 11 is provided with three discharge branch pipes, the positions of the discharge branch pipes correspond to the positions of the three vessel seats 7 one by one, the incubator 1 is a stainless steel member, a through window is arranged on the cabinet door 4, the front surfaces of the atomizing box 5 and the master batch box 6 are provided with liquid level through windows, the device is connected with a power supply, the cabinet door 4 is opened by rotation, the vessels 8 inside the vessel seats 7 are taken out, the base materials are placed inside the vessels 8, the vessels 8 with the base materials are clamped inside the vessel seats 7, the master batch 4 is closed, the master batch is placed inside the cabinet door 6, clear water is added inside the atomizing box 5, and then the vessels inside the master batch box 6 are led into the, vessel seat 7 drives household utensils 8 and rotates, mix 8 inside materials of household utensils, temperature and humidity sensor 9 real-time running detects the humiture of 1 inside of incubator, heating zone 13 operation, to 1 inside heating of incubator, 5 atomizing water liquid of atomizing case, the inside of leading-in incubator 1 through outlet duct 12, when temperature and humidity sensor 9 detected the humiture and reached the limit value, heating zone 13 stop heating, atomizing case 5 stops the humidification, the inside gas of incubator 1 discharges through exhaust net 10, guarantee the circulation of air, the condition of 8 inside materials of household utensils is watched to the transparent window of accessible cabinet door 4, after the microbial cultivation, open cabinet door 4, take out 7 inside household utensils 8 of vessel seat, it can to take out the inside fungus liquid of household utensils 8, and finally, clean household utensils 8, the disconnection power supply can.

In fig. 3: the lower surface of the vessel seat 7 is welded with a rotating rod 14, the inner lower surface of the incubator 1 is provided with a motor 15, the outer surface of the rotating rod 14 is provided with a transmission belt 16, one side of the inner lower surface of the control box 2 is provided with a single chip microcomputer 19, one side of the front surface of the control box 2 is provided with a display screen 17, the front surface of the control box 2 is provided with a control key 18 near the external part of the display screen 17, the output end electrode of the single chip microcomputer 19 is connected with the display screen 17, the control key 18, the motor 15, a temperature and humidity sensor 9 and the input end of the heating belt 13, the output end electrode of the single chip microcomputer 19 is connected with a discharge pipe 11, an air outlet pipe 12 and the input end of an electrode component on the atomization box 5, the vessel seat 7 is fixed above the motor 15, the other two vessel seats 7 are correspondingly arranged on the two sides above the motor 15, the output shaft of bull stick 14 and motor 15 passes through drive belt 16 and rotates and is connected, press down control button 18 and pass through 19 device operations of singlechip, the inside masterbatch of masterbatch case 6 passes through the inside of 11 leading-in household utensils of discharging pipe, motor 15 moves, it rotates to drive bull stick 14 through drive belt 16, bull stick 14 drives the rotation of ware seat 7 with motor 15, ware seat 7 drives the rotation of household utensils 8, mix the inside material of household utensils 8, the humiture of humiture sensor 9 real-time running detection incubator 1 inside, the heating zone 13 moves, to incubator 1 internal heating, 5 atomizing water liquid of atomizing case, through the leading-in inside of incubator 1 of outlet duct 12.

In fig. 4, 5: the lower end of a discharge pipe 11 is provided with a discharge electromagnetic valve 20, the lower end of the discharge pipe 11 is provided with a flow valve 21 close to the external part of the discharge electromagnetic valve 20, the upper end of a discharge pipe 12 is provided with a gas outlet electromagnetic valve 23, the upper end of the discharge pipe 12 is provided with a steam trap 22 close to the position of an atomization box 5, the atomization box 5 is internally provided with an atomization sheet 24, three branch pipes at the lower end of the discharge pipe 11 are provided with the discharge electromagnetic valve 20 and the flow valve 21 respectively, the three branch pipes at the lower end of the discharge pipe 11 correspond to the upper part of a vessel 8 respectively, the atomization box 5 is communicated with the discharge pipe 12 through the steam trap 22, the discharge electromagnetic valve 20 is opened, master batch in a master batch box 6 is guided into the vessel 8 through the discharge pipe 11, the flow valve 21 runs in real time, the liquid flow is detected, when, atomizing water, opening the water mist in the atomizing box 5by the air outlet electromagnetic valve 23, draining water by the steam trap 22, and leading the water mist into the incubator 1 through the air outlet pipe 12.

It should be noted that: the temperature and humidity sensor 9 (model is BYC10-RHT), the singlechip 19 (model is STM32F103C8T6), the motor 15 (model is GM25-25BY), the flow valve 21 (model is SMWF-8), the discharge electromagnetic valve 20 and the air outlet electromagnetic valve 23 are both (model is US-20), the steam trap 22 (model is 1/2NPT), and the atomizing sheet 24 (model is HW-16-112E-EO).

The working principle of the invention is as follows: firstly, taking out the device, supporting the device by supporting legs 3 and placing the device at a using position, connecting the device with a power supply, rotating to open a cabinet door 4, taking out a vessel 8 in a vessel seat 7, placing a base material in the vessel 8, clamping the vessel 8 with the base material in the vessel seat 7, closing the cabinet door 4, placing a master batch in a master batch box 6, adding clear water into the atomization box 5, then pressing a control key 18 to control a discharge electromagnetic valve 20 to open through a singlechip 19, guiding the master batch in the master batch box 6 into the vessel 8 through a discharge pipe 11, operating a flow valve 21 in real time, detecting the liquid flow, when a limit value is reached, closing the discharge electromagnetic valve 20, stopping the supply of the master batch, operating a motor 15, driving a rotating rod 14 to rotate through a driving belt 16, driving the rotating rod 14 and the motor 15 to drive the vessel seat 7 to rotate, driving the vessel 8 to rotate by the vessel seat 7, the temperature and humidity sensor 9 of the materials in the mixing vessel 8 operates in real time to detect the temperature and humidity in the incubator 1, the heating belt 13 operates, heating the inside of the incubator 1, operating the atomizing sheet 24 inside the atomizing box 5 to atomize water, opening the air outlet electromagnetic valve 23 to spray water inside the atomizing box 5, after the water is drained by the steam trap 22, the water is guided into the incubator 1 through the air outlet pipe 12, when the temperature and humidity sensor 9 detects that the temperature and humidity reach a limit value, the heating belt 13 stops heating, the atomization box 5 stops humidifying, the gas in the incubator 1 is discharged through the exhaust net 10 to ensure the circulation of air, the condition of 8 inside materials of household utensils is watched to the window that passes through of accessible cabinet door 4, and after the microbial cultivation, open cabinet door 4, take out 7 inside household utensils 8 of household utensils seat, take out 8 inside fungus liquid of household utensils can, at last, clean household utensils 8, the disconnection power supply can.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The utility model provides a microbial cultivation device for breed environment improvement, includes incubator (1), its characterized in that, control box (2) are installed to the lower extreme of incubator (1), the lower skin weld of control box (2) has supporting legs (3), the front surface of incubator (1) rotates installs cabinet door (4), exhaust net (10) are installed to one side surface upper end of incubator (1), the last surface mounting of incubator (1) has mother's workbin (6), the lower surface through welding of mother's workbin (6) has discharging pipe (11), atomizing box (5) are installed to the position department that the upper surface of incubator (1) faces nearly mother's workbin (6), the upper surface through welding of atomizing box (5) has outlet duct (12), vessel seat (7) are installed to the inside lower extreme of incubator (1), the internally mounted of vessel seat (7) has household utensils (8), temperature and humidity sensor (9) are installed to inside one side of incubator (1), the inside lower surface mounting of incubator (1) has heating tape (13).

2. The microbial cultivation device for improving the cultivation environment according to claim 1, wherein a rotating rod (14) is welded on the lower surface of the vessel seat (7), a motor (15) is installed on the lower surface inside the incubator (1), a transmission belt (16) is installed on the outer surface of the rotating rod (14), a single chip microcomputer (19) is installed on one side of the lower surface inside the control box (2), a display screen (17) is installed on one side of the front surface of the control box (2), and a control key (18) is installed on the outer portion, close to the display screen (17), of the front surface of the control box (2).

3. The microbial cultivation device for improvement of cultivation environment according to claim 1, wherein the discharging electromagnetic valve (20) is installed at the lower end of the discharging pipe (11), the flow valve (21) is installed at the external position of the discharging electromagnetic valve (20) close to the lower end of the discharging pipe (11), the air outlet electromagnetic valve (23) is installed at the upper end of the air outlet pipe (12), the steam trap (22) is installed at the position of the upper end of the air outlet pipe (12) close to the atomization box (5), and the atomization sheet (24) is installed inside the atomization box (5).

4. The microbial bacteria culture device for improving the culture environment according to claim 1, wherein the other end of the air outlet pipe (12) penetrates through the upper surface of the incubator (1) and is inserted into the inner position of the incubator (1), three groups of vessel seats (7) and vessels (8) are arranged, the three groups of vessel seats (7) and the vessels (8) are rotatably arranged at the inner position of the incubator (1), the lower end of the discharge pipe (11) is provided with three discharge branch pipes, and the discharge branch pipes are in one-to-one correspondence with the positions of the three vessel seats (7).

5. The microbial cultivation device for improvement of cultivation environment according to claim 1, wherein the cultivation box (1) is a stainless steel member, the cabinet door (4) is provided with a through window, and the front surfaces of the atomization box (5) and the master batch box (6) are provided with liquid level through windows.

6. The microbial cultivation device for improvement of breeding environment according to claim 2, wherein the output end electrode of the single chip microcomputer (19) is connected with the input ends of the display screen (17), the control key (18), the motor (15), the temperature and humidity sensor (9) and the heating belt (13), and the output end electrode of the single chip microcomputer (19) is connected with the output pipe (11), the air outlet pipe (12) and the input end of the electrode member on the atomization box (5).

7. The microbial cultivation device for improving the cultivation environment according to claim 2, wherein one vessel seat (7) is fixed above the motor (15), the other two vessel seats (7) are correspondingly arranged on two sides above the motor (15), the vessel seats (7) on two sides above the motor (15) are rotatably installed at the inner position of the incubator (1) through a rotating rod (14), and the rotating rod (14) is rotatably connected with the output shaft of the motor (15) through a transmission belt (16).

8. The microbial cultivation device for improvement of cultivation environment according to claim 3, wherein the discharging electromagnetic valve (20) and the flow valve (21) are installed at the three branch pipes at the lower end of the discharging pipe (11), the three branch pipes at the lower end of the discharging pipe (11) correspond to the upper part of the vessel (8) respectively, and the atomizing box (5) is connected with the air outlet pipe (12) through a steam trap (22).