CN111803410A

CN111803410A - Pure natural woody plant extract and preparation method and application thereof

Info

Publication number: CN111803410A
Application number: CN202010493011.3A
Authority: CN
Inventors: 李建武; 阮丽; 王丽鸳; 陈丽芬; 林琳; 李欣
Original assignee: Lishui Liandu Agricultural And Rural Bureau; Zhejiang A&F University ZAFU; Tea Research Institute Chinese Academy of Agricultural Sciences
Current assignee: Lishui Liandu Agricultural And Rural Bureau; Zhejiang A&F University ZAFU; Tea Research Institute Chinese Academy of Agricultural Sciences
Priority date: 2020-06-03
Filing date: 2020-06-03
Publication date: 2020-10-23
Anticipated expiration: 2040-06-03
Also published as: CN111803410B

Abstract

The invention relates to the technical field related to extraction of pure natural woody plants, and particularly provides a pure natural woody plant extract and application thereof. The first aspect of the invention provides a preparation method of a pure natural woody plant extract, which comprises the following steps: (1) adding green tea into pure water, and steaming; (2) after the steam was distilled off, the distillate was retained by passing through a condenser tube. The invention provides a method for extracting pure natural single woody plant which can be used as a soothing agent, and the obtained plant extract has light color, no turbidity, stable storage and faint scent; and has no toxicity and irritation to skin; and can inhibit mast cell degranulation, and has allergy relieving effect.

Description

Pure natural woody plant extract and preparation method and application thereof

Technical Field

The invention relates to the technical field related to extraction of pure natural woody plants, and particularly provides a pure natural woody plant extract and application thereof.

Background

Skin allergy is a common skin disease, and if the skin disease is not treated in time, various complications are easily induced along with the aggravation of the condition of the skin disease, and the daily life is affected. For example, urticaria is a localized edema reaction due to dilation of small blood vessels in the skin and mucous membrane and increased permeability, and the rash usually disappears within 2-24 hours; however, new rashes will recur and the course of disease extends from several days to several months. Skin allergy causes many reasons, such as season changes, environmental pollution, intake of allergic substances, and the like. In recent years, with the continuous emergence of various cosmetics, the barrier protection effect of the skin is gradually weakened, and the phenomenon of skin allergy is serious.

How to reduce the allergy rate of cosmetics is a problem which needs to be solved urgently at present, some enterprises add contraband substances such as glucocorticoid to solve the problem, and long-term use of products containing glucocorticoid can cause severe consequences such as skin dependence and even ulceration. Therefore, the research on the pure natural plant-derived soothing agents is an important research direction at present and has practical significance for the cosmetic industry.

At present, most of the allergy-relieving agents from pure natural plant sources are compound, most of the raw materials are herbal medicines, the dosage is large, the extracting solution is dark in color when being used by adopting a conventional extraction method such as a decoction method, and the active ingredients are reduced, separated out and even deteriorated after being placed for a long time due to complex components and short shelf life, so that the skin-soothing agents are difficult to apply to cosmetics. And many raw materials are medicinal materials, have certain medicinal action more or less, may cause the organism reaction, have certain human safety hidden danger.

Aiming at the defects in the prior art, the invention aims to provide a method for extracting a pure natural single woody plant and utilize the method as a soothing agent.

Disclosure of Invention

In order to solve the above technical problems, the present invention provides a method for preparing a pure natural woody plant extract, comprising the steps of:

(1) adding green tea into pure water, and steaming;

(2) after the steam was distilled off, the distillate was retained by passing through a condenser tube.

As a preferable technical scheme of the invention, the method also comprises the step of freeze-drying the distillate to obtain a powdery concentrated product.

In a preferred embodiment of the present invention, the green tea is selected from any one of pan-fired green tea, roasted green tea, sun-dried green tea and steamed green tea.

As a preferred technical scheme of the invention, in the step (1), the cooking process is carried out in a temperature-controlled cooking pot; preferably, the cooking temperature is not higher than 110 ℃.

As a preferable embodiment of the present invention, in the step (1), the concentration ratio of green tea to pure water is 125 g: 1000-2000 mL.

As a preferable technical scheme of the invention, the temperature of the distillate in the step (2) is 20-40 ℃.

The second aspect of the present invention provides a pure natural woody plant extract prepared according to the preparation method of the pure natural woody plant extract.

In a third aspect of the present invention, a skin care composition comprising the pure natural woody plant extract is provided.

In a fourth aspect, the invention provides a soothing agent prepared from the pure natural woody plant extract.

The fifth aspect of the invention provides a use method of the allelochemicals, wherein the pure natural woody plant extract and pure water are mixed according to the volume ratio of 1: (35-45) in proportion.

Compared with the prior art, the invention prepares the pure natural plant extract by adopting the roasted green tea as the raw material, and has the following advantages:

(1) through a specific cooking process and a specific condensation process, the obtained extract has light color, stable performance and no turbidity;

(2) the prepared pure natural plant extract has small effect on human body medicines;

(3) the prepared pure natural plant extract can be used as a allergy-relieving agent, effectively inhibits the phenomenon of mast cell degranulation, and has the allergy-relieving effect.

Drawings

FIG. 1: mast cell degranulation morphological results;

FIG. 2: and (3) comparing the degranulation morphological results of the mast cells of the samples under different conditions.

Detailed Description

The terms "comprises," "comprising," "including," "has," "having," "contains," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.

The invention provides an extraction method of pure natural single woody plant which can be used as a soothing agent, and the obtained plant extract has light color, no turbidity, stable storage and faint scent; and has no toxicity and irritation to skin; and can inhibit mast cell degranulation, and has allergy relieving effect.

The first aspect of the present invention provides a method for preparing a pure natural woody plant extract, comprising the steps of:

(1) adding green tea into pure water, and steaming;

Preferably, the method for preparing the pure natural woody plant extract further comprises freeze-drying the distillate to obtain a powdered concentrated product; further preferably, the temperature of freeze drying is-45-55 ℃, and the pressure is less than 10 Pa; more preferably, the temperature of the freeze-drying is-50 ℃ and the pressure is less than 10 Pa.

The green tea of the present invention may be any one selected from pan-fired green tea, roasted green tea, sun-dried green tea and steamed green tea according to the drying method thereof.

The pan-fried green tea is fried to be dry by a pan and can be classified into long pan-fried green tea, round pan-fried green tea and flat pan-fried green tea according to the appearance. The Changshou tea is also called eyebrow tea. Round roasted green is like granule, also called Zhu tea. The pan-fired tea is also called flat tea. The quality characteristics of the long fried green are tight strip, green and moist color, durable fragrance, strong taste, yellow and bright soup color and leaf bottom. The round pan-fired has the quality characteristics of round and tight appearance, high fragrance, strong flavor, soaking resistance and the like. The finished product of pan-fried green is flat, smooth, fragrant and delicious, such as Xihu Longjing tea.

Baked green tea, also called baked green tea, is baked in a baking cage and can be classified into strip tea, tip tea, sheet tea, needle tea, etc. according to its shape. Baking green in strips, wherein production is carried out in main tea-producing areas in China; the tip-shaped and sheet-shaped tea is mainly produced in provinces such as Anhui and Zhejiang, and mainly comprises Mabian Yunwu tea, Huangshan Maofeng tea, Taiping Houkui, Tingxi Lanxiang, Liuan Guapian, Jingting Luxue, Tianshan green tea, Gushu purple bamboo shoot, Jianshan green peony, Emei Maofeng tea, Jinshui Cuifeng tea, Xizhou Bifeng, Nannuo Baihao and the like. Such as Huangshan Maofeng: produced in yellow mountain in she county, Anhui. The tea has the advantages of delicate and slightly curled appearance, plump and even buds with sharp hairs, a shape similar to 'sparrow tongue', golden and oily color, commonly known as ivory color, fresh and high-length fragrance, apricot yellow and bright soup color, mellow, fresh and sweet taste, and thick, solid and bright-colored buds and leaves at the bottom of the leaves.

The sun-dried green tea is dried by sunlight and mainly distributed in Hunan, Hubei, Guangdong, Guangxi, Sichuan, Yunnan, Guizhou provinces and other provinces and is produced in a small amount; the tea soup of the sun-dried green tea is thick and has colloid, and is also very resistant to soaking; the taste does not change greatly after ten bubbles are drunk, and the sweeter the taste is.

The steaming green tea uses the steam quantity to destroy the enzyme activity in fresh leaves, and forms the quality characteristics of dark green color of dry tea, light green tea soup and green tea bottom, but the aroma is stuffy and has green smell, the astringency is also heavier, and the tea is not as fresh and cool as the pan-fried green tea.

In the actual preparation process of the green tea extract, the extract obtained by green-firing tea is lighter in color, better in storage stability and better in treatment effect when being used, while the extract obtained by green-firing green tea is darker in color when being used, the color of the extract is darker probably because more nitrogen compounds in the green-firing green tea generate caramel or baking fragrance under the action of heat, and the color of the extract is darker, while the extract obtained by green-firing green tea can be layered and precipitated during the storage process, and colloidal molecules can agglomerate to form precipitates during the storage process; the allergy relieving effect of the steamed green tea is a little lower, which is probably because the loss of the ingredients of the steamed green tea obtained by steam under the high-temperature condition of the steam in the early stage causes the effective ingredients obtained in the distillation stage to be relatively lower than that of other types of green tea, thereby affecting the allergy relieving effect.

In addition, the applicant finds in the experimental process that the production place of the baked green tea has no influence on the color, the allergy-relieving effect and the stability of the product basically, but the aroma extracted from the baked green tea is better.

The invention does not require any particular manufacturer of roasted green tea, and is well known to those skilled in the art.

Preferably, the concentration ratio of green tea to pure water in step (1) is 125 g: 1000-2000 mL; further preferably, the concentration ratio of green tea to pure water is 125 g: 1200-1800 mL; further preferably, the concentration ratio of green tea to pure water is 125 g: 1400-1600 mL; more preferably, the concentration ratio of green tea to pure water is 125 g: 1500 mL. The applicant found in the experimental process that when the concentration ratio of green tea to pure water is controlled to 125 g: 1400-1600 mL, the obtained extract has better allergy relieving effect, and at this concentration, the polarity of active ingredients in green tea, such as various aromatic ketones, phenols, esters, alcohols, etc., in the system is balanced, which is beneficial to the precipitation of the active ingredients, while when the water content is low, the precipitation rate of substances with higher polarity is reduced, and when the water content is high, the precipitation rate of substances with low polarity is reduced, thereby affecting the balance of the active ingredients in the extract and failing to exert the optimal allergy relieving effect.

Preferably, the cooking process is carried out in a temperature controlled cooking pan; further preferably, the cooking temperature is not higher than 110 ℃; further preferably, the roasted green tea is wrapped by medical gauze in the cooking process, because applicants find that the cooking temperature needs to be strictly controlled not to be higher than 110 ℃ in the experimental process, and the roasted green tea needs to be used by the medical gauze, otherwise, the phenomenon of pot overflow can occur, the obtained finished product has turbid and unstable color, can be layered or precipitated in the storage process, and greatly reduces the allergy relieving effect of the obtained product, which may be that when the temperature is higher than 110 ℃, the cooking system generates bubbles under the action of water, precipitates, water vapor and steam of active ingredients, the bubbles are accumulated, and overflow under the action of pressure to cause the pot overflow phenomenon, and simultaneously, under the action of external pressure, part of liquid enters a condensation pipe and is mixed into the distillate to cause the turbidity of the distillate or the reduction of the stability, and the allergy relieving effect is reduced; when the temperature is controlled and the medical gauze is used for wrapping, the number of bubbles of the system is reduced, and the accumulation of the bubbles is avoided, so that the phenomenon of overflowing is avoided.

Preferably, the green-baked green tea is pretreated before the step (1), namely, the green-baked green tea is soaked in pure water for 1.5-2.5 hours, so that the allergy relieving effect of the product can be further improved, and the green-baked green tea is steamed and boiled after being soaked, so that a water channel penetrates through the veins of the green tea, the precipitation of effective components is facilitated, the concentration of the effective components is improved, and the allergy relieving effect of the product is further improved.

Preferably, in the step (2), 4 loops of condensation pipes are adopted, and further preferably, the length of the 4 loops of condensation pipes is 3-4 m; more preferably 3.76 m.

In the experimental process, the applicant finds that the temperature of the condensation pipe and the distillate is strictly controlled to be 20-30 ℃ in the step (2), otherwise, the allergy relieving effect of the product can be greatly reduced, when the temperature is controlled to be 20-30 ℃, under the condition of the condensation area and the flow of the condensation pipe with a certain specification, the effective allergy relieving component can be well condensed to form the effective component of the distillate, and when the temperature is higher, such as 30-40 ℃, the temperature is higher than the condensation temperature of the effective component, so that the content of the effective component in the distillate is lower, and the allergy relieving effect of the product is greatly reduced.

In a third aspect of the present invention, a skin care composition comprising the pure natural woody plant extract is provided. For example, as skin care compositions, skin care cosmetic compositions can be formulated in a variety of forms, including, for example, water/oil or oil/water emulsion type cosmetics, creams, lotions, toilet water, essential oils; oily cosmetics, skin moisturizing creams, lipsticks, foundation creams, skin cleansing compositions, hair tonics, hair styling compositions, hair grooming compositions, hair growth stimulants, and the like.

The fifth aspect of the invention provides a use method of the allelochemicals, wherein the pure natural woody plant extract and pure water are mixed according to the volume ratio of 1: (35-45) mixing and using; preferably, the pure natural woody plant extract and pure water are mixed according to a volume ratio of 1: mixing at a ratio of 40.

Example 1

Example 1 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2 hours; adding 125g of the baked green tea into 1500mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea; the baked green tea is Fujian baked tea;

(2) after steam is evaporated, the steam passes through 4 loops of condensation tubes with the length of 3.76m, distillate is reserved, and the temperature of the condensation tubes and the distillate is controlled to be 20-30 ℃;

(3) the output speed of the distillate is 1L/h, and the distillate is freeze-dried to obtain a powdery concentrated product; the temperature of freeze drying is-50 deg.C, and the pressure is less than 10 Pa.

Example 2

Embodiment 2 of the present invention provides a pure natural woody plant extract, which is prepared by the following steps:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2.5 hours; adding 125g of baked green tea into 1600mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea; the baked green tea is Fujian baked tea;

Example 3

Example 3 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 1.5 hours; adding 125g of the green-dried green tea into 1400mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the process of cooking the green-dried green tea; the baked green tea is Fujian baked tea;

Example 4

Example 4 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2 hours; adding 125g of the baked green tea into 1500mL of pure water, and then cooking in a temperature-controlled steamer, wherein the temperature is controlled at 120 ℃, and medical gauze is used for wrapping in the steaming and cooking process of the baked green tea; the baked green tea is Fujian baked tea;

Example 5

Example 5 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2 hours; adding 125g of the green-dried green tea into 1500mL of pure water, and then cooking in a temperature-controlled steamer, wherein the temperature is controlled within 110 ℃; the baked green tea is Fujian baked tea;

Example 6

Example 6 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(2) after steam is evaporated, the steam passes through 4 loops of condensation tubes with the length of 3.76m, distillate is reserved, and the temperature of the condensation tubes and the distillate is controlled to be 30-40 ℃;

Example 7

Example 7 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the pan-fired green tea, namely soaking the pan-fired green tea in pure water for 2 hours; adding 125g of the baked green tea into 1500mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea;

Example 8

Example 8 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating sun-dried green tea, namely soaking in pure water for 2 hours; adding 125g of the baked green tea into 1500mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea;

Example 9

Example 9 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the steamed green tea, namely soaking the steamed green tea in pure water for 2 hours; adding 125g of the baked green tea into 1500mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea;

Example 10

Example 10 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2 hours; adding 125g of baked green tea into 2000mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the baking and cooking process of the green tea; the baked green tea is Fujian baked tea;

Example 11

Example 11 of the present invention provides a pure natural woody plant extract, which is prepared by the following method:

(1) pretreating the green-baked green tea, namely soaking the green-baked green tea in pure water for 2 hours; adding 125g of the baked green tea into 900mL of pure water, then cooking in a temperature-controlled steamer, controlling the temperature within 110 ℃, and wrapping by medical gauze in the process of cooking the baked green tea; the baked green tea is Fujian baked tea;

Performance evaluation

The concentrated product in the form of powder obtained in examples 1 to 11 was dissolved in pure water at a ratio of 1:40 and used, and the following performance tests were carried out:

1. according to the technical specification of cosmetic safety (2015 edition) issued by the State food and drug administration, the safety technical center of the Industrial products of the Tianjin entry and exit inspection and quarantine bureau carries out the sanitary safety test on the products obtained in the above embodiments 1-3, and the toxicology test result shows that: the test result of the acute oral toxicity test is actually non-toxic, the test result of the acute eye irritation test is nonirritant under the condition of no-flushing test, the test result of the skin irritation test for many times is nonirritant, the contents of lead, arsenic, mercury and cadmium all meet the requirements of technical standards for cosmetic safety (2015 edition), and the residual quantities of pesticides and related chemicals in the product are not detected according to the national food safety standard (GB 23200.8-2016).

2. According to the technical specification for cosmetic safety (2015 edition) issued by the State food and drug administration, we performed a human skin closed patch test on the product obtained in example 1, and the subjects: the total of 33 people, 27 women and 6 men, the age of 21-43 years, meet the volunteer selection criteria of the subject. The results of the human skin patch test (tables 1 and 2) show that only 2 of 33 people showed suspicious responses at 0.5h, and that such responses disappeared at both 24h and 48 h.

TABLE 1 skin closed Patch test skin response grading Standard

TABLE 2 test results of human skin patches

The negative control (blank) described in table 2 refers to a blank in which no product was used.

3. The product obtained in example 1 was evaluated for anti-inflammatory and anti-allergic efficacy-1: the results are shown in Table 3 and FIG. 1; after C48/80 stimulation (NC group), cells showed a clear degranulation morphology compared to the unstimulated (BC group); after the stabilizer cromolyn sodium (PC group) is added for treatment, the number of degranulated cells in a visual field is remarkably reduced, which indicates that a C48/80 induction stimulation mast cell degranulation model is successfully established and can be used for screening the degranulated stabilizer, compared with an NC group, the degranulation phenomenon of the product after the product acts on the mast cells is remarkably inhibited (P-value is less than 0.01), namely the product can inhibit the degranulation phenomenon of the mast cells and has the allergy relieving effect.

TABLE 3 examination result of degranulation rate of mast cell degranulation

Remarking:

negative Control (NC) C48/80 (10. mu.g/mL);

the Positive Control (PC) was C48/80 (10. mu.g/mL) +1mg/mL disodium cromoglycate;

the sample (S) was the sample obtained in example 1.

4. The products obtained in example 1, example 4 and example 6 were evaluated for anti-inflammatory and anti-allergy-relieving efficacy-2: the results are shown in table 4 and fig. 2 (both magnification is 400 times), the product obtained in example 1 can significantly inhibit the degranulation phenomenon after acting on mast cells (P-value < 0.01), i.e. the degranulation phenomenon of mast cells can be inhibited, and the desensitization effect of example 1 is better, while examples 4 and 6 also have a certain inhibition effect on the degranulation phenomenon of mast cells, but the effect is weaker than that of example 1, and P of example 4 is greater than 0.01.

TABLE 4 comparison of the results of examination of the degranulation rate of mast cells from samples prepared under different conditions

Group of	Mean value	SD value	P value (VS BC)
				Blank Control (BC)	0.00％	0.00％	/
Sample (S1)	74.45％	2.68％	0.007
				Sample (S2)	87.72％	3.80％	0.004
Sample (S3)	87.33％	3.37％	0.900

Remarking:

the sample (S1) was the sample obtained in example 1;

the sample (S2) was the sample obtained in example 6;

the sample (S3) was the sample obtained in example 4.

5. Physical properties of the samples obtained in the examples were measured: observing the color of the obtained product, and dividing the experimental result into: light, darker and darker color; the stability of the obtained product was observed: placing the obtained product at 25 deg.C and 25% humidity for two months, and observing whether precipitation or delamination occurs; whether the appearance of the obtained product is turbid or not is observed, and the experimental result is shown in table 5.

TABLE 5

The pure natural woody plant soothing agent for cosmetics, which is effective, stable, light in color and small in medicinal effect on human bodies, is prepared by adopting a specific process and raw materials.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Where numerical ranges are used in the claims, also included are sub-ranges within them, and variations in these ranges are also to be construed as being covered by the appended claims where possible.

Claims

1. A preparation method of a pure natural woody plant extract is characterized by comprising the following steps:

(1) adding green tea into pure water, and steaming;

2. The method of claim 1, further comprising freeze drying the distillate to obtain a concentrated powder.

3. The method of producing a pure natural woody plant extract according to claim 1 or 2, wherein the green tea is selected from any one of pan-fired green tea, roasted green tea, sun-dried green tea and steamed green tea.

4. The method for preparing the pure natural woody plant extract according to claim 3, wherein in the step (1), the cooking process is performed in a temperature-controlled cooking pot; preferably, the cooking temperature is not higher than 110 ℃.

5. The method for preparing the pure natural woody plant extract according to claim 3 or 4, wherein the concentration ratio of green tea to pure water in the step (1) is 125 g: 1000-2000 mL.

6. The method for preparing the pure natural woody plant extract according to claim 3 or 4, wherein the temperature of the distillate in the step (2) is 20 to 40 ℃.

7. A pure natural woody plant extract prepared according to the method for preparing a pure natural woody plant extract of any one of claims 1 to 6.

8. A skin care composition comprising the pure natural woody plant extract according to claim 8.

9. A soothing agent prepared from the pure natural woody plant extract of claim 8.

10. A method of using a soothing agent according to claim 9 wherein the pure natural woody plant extract is mixed with pure water in a volume ratio of 1: (35-45) in proportion.