CN111781197B - Rapid sepsis detection kit and process - Google Patents
Rapid sepsis detection kit and process Download PDFInfo
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- CN111781197B CN111781197B CN202010677968.3A CN202010677968A CN111781197B CN 111781197 B CN111781197 B CN 111781197B CN 202010677968 A CN202010677968 A CN 202010677968A CN 111781197 B CN111781197 B CN 111781197B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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Abstract
The invention discloses a rapid sepsis detection kit which can realize multi-group detection and avoid the influence of external environment on a detection result. The sepsis rapid detection kit comprises a box body, wherein vertical detection cavities which are uniformly distributed along the transverse direction are arranged on the box body; a discharge channel communicated with the transverse main channel is arranged on the vertical detection cavity; one end of the main channel is provided with a micro fan, and the other end of the main channel is provided with a micro circulating pump; a cleaning solution storage tube, a diluent storage tube, an injector and a dilution test tube are arranged in the box body; the box body is provided with a detection reagent injector and a detection probe. Adopt this sepsis rapid detection kit to include the box body, can realize that the multiunit detects, be convenient for improve and detect the accuracy, can realize the washing to detecting the chamber, be convenient for realize used repeatedly.
Description
Technical Field
The invention relates to the field of disease detection, in particular to a sepsis rapid detection kit.
Background
It is known that: sepsis refers to the systemic inflammatory response syndrome system in inflammatory response syndrome, SIRS, caused by infection with clinically proven presence of bacteria or highly suspicious foci of infection. Although sepsis is caused by infection, once it occurs, its development follows its own pathological course and law, so it is essentially the body's response to infectious agents.
There are several existing ways of detecting sepsis, such as the kit, method and apparatus disclosed in chinese patent application 201580013411.7 for analysing biological samples from animals to predict (before symptoms appear) and monitor the development of sepsis, which makes use of biomarker signatures, in particular biomarker signatures that can provide a mean predictive accuracy of at least 92% to distinguish sepsis from non-sepsis development. Although the method has high detection accuracy, the method is finished in a laboratory, so the method has the disadvantages of complex operation, long time consumption and low efficiency.
Another kind of detection mode is as the kit that china patent application 201910547140.3 disclosed a short-term test sepsis, including the plastics box body, the inside sliding connection of plastics box body has the pull board, the preceding table wall of pull board is provided with the handle, and has seted up mounting groove, first draw-in groove and second draw-in groove on the pull board, the mounting groove is located the one end of first draw-in groove and second draw-in groove, the second draw-in groove is located one side of first draw-in groove, the inside of mounting groove is provided with chip groove and outer temperature detector be provided with two gasbags of symmetry on the bottom plate of chip box, paste on the bottom plate in the bottom of two gasbags, form a plane that can place chip box and treater, outer temperature detector is convenient for detect indoor temperature to show through the display. The user of being convenient for watches indoor temperature, prevents that indoor temperature is too high or low excessively, influences the accurate nature that the kit detected.
Although the detection mode can overcome the influence of the external environment on the detection result, the detection mode can only carry out single monitoring and cannot form effective contrast detection, so that the accuracy of the detection result cannot be ensured.
Disclosure of Invention
The invention aims to solve the technical problem of providing a rapid sepsis detection kit which can realize multi-group detection and can avoid the influence of external environment on a detection result.
The technical scheme adopted by the invention for solving the technical problems is as follows: the sepsis rapid detection kit comprises a box body, wherein vertical detection cavities which are uniformly distributed along the transverse direction are arranged on the box body; a rubber plug is arranged at the upper end of the vertical detection cavity;
a discharge channel is arranged at the lower end of the vertical detection cavity; the discharge channel is provided with a regulating valve;
a transverse main channel penetrating through two sides of the box body is arranged in the middle of the box body; one end of the main channel is provided with a micro fan, and the other end of the main channel is provided with a micro circulating pump; the discharge channel is communicated with the transverse main channel; a storage battery is arranged in the box body; a second valve is arranged between the micro fan and the main channel; a first valve is arranged between the micro circulating pump and the main channel;
the lower end of the box body is provided with a first circular storage cavity with an opening at one side of the box body; a first plug board is arranged at one end, provided with an opening, of the first storage circular cavity; the first storage circular cavity has at least two; the first inserting plate is inserted into the box body to seal the first storage circular cavity;
the lower end of the box body is provided with a second circular storage cavity and a third circular storage cavity, wherein the second circular storage cavity and the third circular storage cavity are provided with openings on the other side of the box body; one end of the second storage circular cavity is provided with a second plug board; the second plug board is inserted into the box body to seal the second circular storage cavity and the third circular storage cavity;
a vertical round storage cavity is arranged in the middle of the lower end of the box body; the lower end of the vertical round storage cavity is provided with a third inserting plate; the third inserting plate is inserted into the sealed vertical round storage cavity in the box body;
a cleaning liquid storage pipe is arranged in the first circular storage cavity; a diluent storage pipe is arranged in the vertical round storage cavity; a syringe is arranged in the second storage circular cavity, and a dilution test tube is arranged in the third storage circular cavity;
the back of the box body is provided with a processor and a detection probe which is inserted into the vertical detection cavity and is used for detecting temperature and pressure; the detection probe is electrically connected with the processor;
a transparent observation window for observing the vertical detection cavity is arranged on the front side of the box body; a color comparison card is arranged on one side of the transparent observation window;
the front side of the box body is provided with a detection reagent injector which is inserted into the upper part of the vertical detection cavity; a small heating resistor is arranged on one side of the vertical detection cavity in the box body; the front surface of the box body is provided with a touch display screen;
the micro fan, the micro circulating pump, the small heating resistor, the touch display screen and the detection probe are respectively and electrically connected with the processor;
the storage battery provides electric energy for the micro fan, the micro circulating pump, the small heating resistor, the touch display screen, the detection probe and the processor.
Specifically, the detection reagent injector adopts a micro-syringe; the detection reagent injector is provided with a tube body, a pushing head, an injection needle and a connector; the connector is in threaded fit with the box body.
Specifically, an induction probe is arranged on the detection probe; the induction probe is in threaded fit with the box body;
the inductive probe is provided with a temperature sensor and a pressure sensor; the temperature sensor and the pressure sensor are positioned in the vertical detection cavity; and the front end of the induction probe is provided with a detection chip.
Specifically, one side of the box body is provided with a charging borrowing port of the storage battery.
Specifically, the front of box body is provided with the transparent observation window in first storage circular chamber, vertical storage circular chamber, second storage circular chamber and third storage circular chamber.
The invention has the beneficial effects that: the rapid sepsis detection kit is provided with a plurality of detection reagent chambers, so that a plurality of groups of detections can be simultaneously realized, and detection results can be compared, thereby ensuring and improving the detection accuracy.
Secondly, because the micro-fan and the reagent injector in the form of a syringe are arranged, the air pressure in the detection cavity can be adjusted through the micro-fan and the reagent injector; meanwhile, the temperature in the detection cavity can be detected through the temperature sensor, and the heating resistor is arranged, so that the reaction temperature can be conveniently adjusted. Therefore, the external environment can influence the detection result.
And thirdly, the cleaning of the detection cavity can be realized by arranging the cleaning liquid and the circulating pump, so that the repeated use is convenient to realize.
Drawings
FIG. 1 is a perspective view of a rapid sepsis detection kit in an embodiment of the present invention;
FIG. 2 is a front view of the rapid sepsis detection kit in the example of the present invention;
FIG. 3 is a rear view of the rapid sepsis detection kit in an embodiment of the present invention;
FIG. 4 is a top view of a rapid sepsis detection kit in an embodiment of the present invention;
FIG. 5 is a sectional view A-A of FIG. 4;
FIG. 6 is a perspective view of a detector reagent injector in an embodiment of the invention;
FIG. 7 is a schematic diagram of a detector reagent injector in an embodiment of the invention;
FIG. 8 is a perspective view of a detection probe in an embodiment of the present invention.
Detailed Description
The invention is further illustrated with reference to the following figures and examples.
As shown in fig. 1 to 8, the sepsis rapid detection kit comprises a kit body 1, wherein vertical detection chambers 2 which are uniformly distributed along the transverse direction are arranged on the kit body 1; the upper end of the vertical detection cavity 2 is provided with a rubber plug 7;
the lower end of the vertical detection cavity 2 is provided with a discharge channel 3; the discharge channel 3 is provided with a regulating valve 4;
a transverse main channel 8 penetrating through two sides of the box body 1 is arranged in the middle of the box body 1; one end of the main channel 8 is provided with a micro fan 13, and the other end is provided with a micro circulating pump 11; the discharge channel 3 communicates with the main channel 8;
a storage battery 15 is arranged in the box body 1; a second valve 10 is arranged between the micro fan 13 and the main channel 8; a first valve 9 is arranged between the micro circulating pump 11 and the main channel 8;
the lower end of the box body 1 is provided with a first circular storage cavity 17 with an opening at one side of the box body 1; a first insert plate 18 is arranged at one end of the first round cavity 17 with an opening; the first storage circular cavity 17 has at least two; the first inserting plate 18 is inserted into the box body 1 to seal the first circular storage cavity 17;
the lower end of the box body 1 is provided with a second circular storage cavity 24 and a third circular storage cavity 23 which are provided with openings at the other side of the box body 1; a second insert plate 26 is provided at one end of the second circular storage cavity 24; the second inserting plate 26 is inserted into the box body 1 to seal the second circular storage cavity 24 and the third circular storage cavity 23;
a vertical round storage cavity 20 is arranged in the middle of the lower end of the box body 1; the lower end of the vertical round storage cavity 20 is provided with a third inserting plate 21; the third inserting plate 21 is inserted into the box body 1 to seal the vertical round storage cavity 20;
a cleaning liquid storage pipe 19 is arranged in the first circular storage cavity 17; a diluent storage tube 22 is arranged in the vertical round storage cavity 20; a syringe 27 is arranged in the second circular storage cavity 24, and a dilution test tube 25 is arranged in the third circular storage cavity 23;
the back of the box body 1 is provided with a processor 29 and a detection probe 5 which is inserted into the vertical detection cavity 2 and is used for detecting temperature and pressure; the detection probe 5 is electrically connected with the processor 29;
the front surface of the box body 1 is provided with a transparent observation window 31 for observing the vertical detection cavity 2; a color comparison card 30 is arranged on one side of the transparent observation window 31;
the front surface of the box body 1 is provided with a detection reagent injector 6 inserted into the upper part of the vertical detection cavity 2; a small heating resistor 33 is arranged on one side of the vertical detection cavity 2 in the box body 1; the front surface of the box body 1 is provided with a touch display screen 32;
the micro fan 13, the micro circulating pump 11, the small heating resistor 33, the touch display screen 32 and the detection probe 5 are respectively electrically connected with the processor 29;
the storage battery 15 provides electric energy for the micro fan 13, the micro circulating pump 11, the small heating resistor 33, the touch display screen 32, the detection probe 5 and the processor 29.
Specifically, in the application process, the regulating valve 4 and the second valve 10 are firstly opened, and the rubber plug 7 is taken down; so that the vertical detection cavity 2 is communicated with the micro fan 13; then starting the micro fan 13 to enable the micro fan to exhaust air, enabling the vertical detection cavity 2 and the pipeline to be cooled, and judging whether the temperature at the moment is lower than the detection reaction temperature or not through the temperature displayed by the touch display screen 32; the micro-fan 13 is braked below the detection reaction temperature and then the regulating valve 4 and the first valve 10 are closed.
Then the syringe 27, the dilution test tube 25 and the diluent storage tube 22 are removed, and the blood sample is diluted; then injecting the diluted blood sample into the vertical detection cavity 2; and the rubber plug 7 is plugged; then, the detection reagent is completely injected into the vertical detection cavity 2 through the detection reagent injector 6; whether atmospheric pressure satisfies the detection atmospheric pressure is judged through the atmospheric pressure that touch display screen 32 shows this moment, because vertical detection chamber 2 is in encapsulated situation when injecting into detect reagent, injects detect reagent atmospheric pressure and can rise this moment, takes out the partial gas in vertical detection chamber 2 through detect reagent injector 6 to make atmospheric pressure reduce, reach the requirement that accords with the detection reaction.
Then, by adding a small resistor 33, the vertical detection chamber 2 is heated, so that the temperature rise meets the detection requirement. At this time, the blood sample reacts with the detection reagent, the color after the reaction is observed through the transparent observation window 31, and then the blood sample is compared with the colorimetric card 30, sepsis is detected according to the change of the color, and thus the detection is realized. And after the detection is finished, comparing and analyzing the multiple groups of detection results to obtain a final monitoring result.
After detection is finished, opening the regulating valve 4 and the first valve 9, and taking down the rubber plug 7; the circulation pump 11 is activated to draw the blood sample after the test.
Then, the cleaning liquid storage tube 19 is taken out; the rubber plug 7 is plugged in the vertical detection cavity 2, and the rubber plug 7 on one vertical detection cavity 2 is taken down; then the cleaning liquid is sent into the vertical detection cavity 2; and the circulating pump 11 is communicated with the upper part of the vertical detection cavity 2 through a conduit, and the vertical detection cavity 2 is circularly cleaned through cleaning liquid. After the cleaning is finished, taking down the guide pipe and discharging the cleaning solution; then plug up this vertical detection chamber 2 with rubber end cap 7, take off the rubber end cap 7 of another vertical detection chamber 2, repeat above-mentioned step, analogize with this, finally accomplish the initial cleaning to vertical detection chamber 2, add new washing liquid at last, carry out the washing of second time, accomplish the washing to detecting the chamber.
After cleaning, taking down the rubber plug 7, opening the second valve 10, closing the first valve 9, starting the micro fan 13, and air-drying the detection cavity through the micro fan 13; and then can be used for the next test.
In conclusion, the rapid sepsis detection kit provided by the invention is provided with a plurality of detection reagent chambers, so that a plurality of groups of detections can be simultaneously realized, and the detection results can be compared, thereby ensuring and improving the accuracy of the detection.
Secondly, because the micro-fan and the reagent injector in the form of a syringe are arranged, the air pressure in the detection cavity can be adjusted through the micro-fan and the reagent injector; meanwhile, the temperature in the detection cavity can be detected through the temperature sensor, and the heating resistor is arranged, so that the reaction temperature can be conveniently adjusted. Therefore, the external environment can influence the detection result.
And thirdly, the cleaning of the detection cavity can be realized by arranging the cleaning liquid and the circulating pump, so that the repeated use is convenient to realize.
In order to facilitate installation, guarantee the tightness of the vertical detection cavity 2 and facilitate adjustment of air pressure in the vertical detection cavity 2, further, the detection reagent injector 6 adopts a micro-syringe; the detection reagent injector 6 has a tube 61, a pusher 62, an injection needle 63, and a connector 64; the connector 64 is in threaded fit with the box body 1.
In order to facilitate installation and ensure the tightness of the vertical detection cavity 2, an induction probe 51 is arranged on the detection probe 5; the induction probe 51 is in threaded fit with the box body 1;
the inductive probe 51 is provided with a temperature sensor 52 and a pressure sensor 53; the temperature sensor 52 and the pressure sensor 53 are positioned in the vertical detection cavity 2; the front end of the induction probe 51 is provided with a detection chip 54.
The detection chip 54 may be integrated with a PH detection sensor and a corresponding ion detection sensor, thereby facilitating more comprehensive analysis of the detection result.
For charging, it is preferable that a charging inlet 16 of the storage battery 15 is provided at one side of the case 1.
In order to facilitate observation, the front surface of the box body is provided with a transparent observation window of a first circular storage cavity, a vertical circular storage cavity, a second circular storage cavity and a third circular storage cavity for storing the state of articles in each storage cavity.
Claims (4)
1. The rapid sepsis detection kit is characterized in that: the detection device comprises a box body (1), wherein vertical detection cavities (2) which are uniformly distributed along the transverse direction are arranged on the box body (1); a rubber plug (7) is arranged at the upper end of the vertical detection cavity (2);
the lower end of the vertical detection cavity (2) is provided with a discharge channel (3); the discharge channel (3) is provided with an adjusting valve (4);
a transverse main channel (8) penetrating through two sides of the box body (1) is arranged in the middle of the box body (1); one end of the main channel (8) is provided with a micro fan (13), and the other end is provided with a micro circulating pump (11); the discharge channel (3) is communicated with a transverse main channel (8); a storage battery (15) is arranged in the box body (1); a second valve (10) is arranged between the micro fan (13) and the main channel (8); a first valve (9) is arranged between the micro circulating pump (11) and the main channel (8);
the lower end of the box body (1) is provided with a first circular storage cavity (17) with an opening at one side of the box body (1); one end of the first storage circular cavity (17) with an opening is provided with a first insert plate (18); the first storage circular cavity (17) has at least two; the first inserting plate (18) is inserted into the box body (1) to seal the first storage circular cavity (17);
the lower end of the box body (1) is provided with a second circular storage cavity (24) and a third circular storage cavity (23) which are provided with openings at the other side of the box body (1); one end of the second storage circular cavity (24) is provided with a second inserting plate (26); the second inserting plate (26) is inserted into the box body (1) to seal the second circular storage cavity (24) and the third circular storage cavity (23);
a vertical round storage cavity (20) is arranged in the middle of the lower end of the box body (1); the lower end of the vertical round storage cavity (20) is provided with a third inserting plate (21); the third inserting plate (21) is inserted into the box body (1) to seal the vertical round storage cavity (20);
a cleaning solution storage pipe (19) is arranged in the first circular storage cavity (17); a diluent storage pipe (22) is arranged in the vertical round storage cavity (20); a syringe (27) is arranged in the second circular storage cavity (24) and a dilution test tube (25) is arranged in the third circular storage cavity (23);
the back of the box body (1) is provided with a processor (29) and a detection probe (5) which is inserted into the vertical detection cavity (2) and is used for detecting temperature and pressure; the detection probe (5) is electrically connected with the processor (29);
a transparent observation window (31) for observing the vertical detection cavity (2) is arranged on the front surface of the box body (1); a color comparison card (30) is arranged on one side of the transparent observation window (31);
a detection reagent injector (6) inserted into the upper part of the vertical detection cavity (2) is arranged on the front surface of the box body (1); a small heating resistor (33) is arranged on one side of the vertical detection cavity (2) in the box body (1); the front surface of the box body (1) is provided with a touch display screen (32);
the micro fan (13), the micro circulating pump (11), the small heating resistor (33), the touch display screen (32) and the detection probe (5) are respectively and electrically connected with the processor (29);
the storage battery (15) provides electric energy for the micro fan (13), the micro circulating pump (11), the small heating resistor (33), the touch display screen (32), the detection probe (5) and the processor (29);
the detection reagent injector (6) adopts a micro-injector; the detection reagent injector (6) is provided with a tube body (61), a push head (62), an injection needle (63) and a connecting head (64); the connector (64) is in threaded fit with the box body (1).
2. The rapid sepsis detection kit according to claim 1, wherein: an induction probe (51) is arranged on the detection probe (5); the induction probe (51) is in threaded fit with the box body (1);
the induction probe (51) is provided with a temperature sensor (52) and a pressure sensor (53); the temperature sensor (52) and the pressure sensor (53) are positioned in the vertical detection cavity (2); the front end of the induction probe (51) is provided with a detection chip (54).
3. The rapid sepsis detection kit according to claim 2, wherein: one side of the box body (1) is provided with a charging interface of a storage battery (15).
4. A rapid sepsis detection kit according to claim 3, characterized in that: the front of box body (1) is provided with the transparent observation window in first storage circular chamber (17), vertical storage circular chamber (20), second storage circular chamber (24) and third storage circular chamber (23).
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| CN113311149A (en) * | 2021-05-28 | 2021-08-27 | 武汉优恩生物科技有限公司 | A device for immunochromatography |
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