CN111771449A - Application method of micro-nano bubbles in rice seedling culture - Google Patents
Application method of micro-nano bubbles in rice seedling culture Download PDFInfo
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Abstract
The application discloses an application method of micro-nano bubbles in rice seedling raising, belongs to the technical field of crop planting improvement, and comprises two steps of accelerating germination of rice seeds by adopting the micro-nano bubbles and promoting growth of rice seedlings by adopting the micro-nano bubbles, namely firstly soaking the rice seeds by adopting micro-nano bubble water for 24 hours, then discharging the micro-nano bubble water, and standing for 24 hours under a closed condition to obtain germinated rice seeds; putting the obtained germinated rice seeds into a rice seedling raising tray, then placing the rice seedling raising tray into a container filled with a rice culture solution, controlling the temperature to be 28 ℃ for 10-21 days after the rice culture solution is immersed in the germinated rice seeds and is 0.1-0.5cm higher than the surface of the germinated rice seeds, and performing micro-nano bubble treatment on the rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generation equipment according to a certain frequency and cycle times in the cultivation process to finally obtain the rice seedlings with strong roots.
Description
Technical Field
The application belongs to the technical field of crop planting improvement, and particularly relates to an application method of micro-nano bubbles in rice seedling raising.
Technical Field
Rice is one of the most important food crops in the world, and more than 30 hundred million people in the world depend on the rice as main food. In view of environmental climate change, population expansion and other problems, it is estimated that rice needs to be increased by 40% by 2030 years to meet the demand. The growth-promoting character of rice is always the key technical innovation field of crop production. As a crop with a growth cycle having strong dependence on water, the water environment has great influence on the growth and yield of rice. Taking the seedling stage as an example, the rice seeds need to be soaked and germinated, and the growth and differentiation of the roots and stems of the seedlings at the initial stage also need to be completed in the water environment. Factors such as oxygen content and nutrient element components of the water body are crucial to the respiration, development and nutrient absorption of the root system, and influence the redox activity, energy accumulation, tillering development and the like of plants on the upper part of the water surface.
In natural environment, a water body absorbs and fuses gaseous bubbles with different sizes through physical events such as waterfalls and riverbank impact, the bubbles exist mostly as large-diameter bubble particles, the contact specific surface area with the water body is small, the self rapid rise and the retention time are short, and therefore the oxygen dissolving capacity is poor. Foreign studies have shown that the smaller the bubble particle size, the stronger the oxygen dissolving capacity. The micro-nano bubble technology is to generate bubbles with the size of micron and nano-scale in water. Due to the special particle size level, the micro-nano bubbles are not influenced by the solubility of air in water and are not limited by external conditions such as temperature, pressure and the like, so that the oxygen content in the water body is obviously increased and even reaches several times of the oxygen solubility in a common aqueous solution. The concept of related micro-nano bubbles was first proposed in 1994 by Parker et al when studying long-range hydrophobic interactions. The field has been developed for more than 20 years, and gradually attracts wide attention and is deeply applied to various fields, such as the field of water environment treatment and improvement and the like. However, the application of the micro-nano bubbles in the seedling raising stage of the grain crops still belongs to the blank at home and abroad, and has excellent development potential.
Disclosure of Invention
In order to use the micro-nano bubbles for rice seedling raising, the application method of the micro-nano bubbles in rice seedling raising is provided, and the application method adopts the synergistic effect of the micro-nano bubbles on rice seed germination accelerating and the micro-nano bubbles on rice seedling growth promoting, so that finally obtained rice seedlings have the characteristic of strong roots and strong seedlings.
Technical principle of the present application
The seeds can be promoted to germinate after being soaked by the micro-nano bubble water, and in addition, the increase of the synthesis amount of the plant growth hormone gibberellin in the rice seedlings can be promoted after the rice culture solution is treated by the micro-nano bubbles, so that the growth of roots and leaves of the germinated rice is further promoted in a synergistic manner, and the rooting and seedling strengthening of the rice seeds are achieved.
Technical scheme of the application
The application method of the micro-nano bubbles in rice seedling raising comprises the two steps of accelerating germination of rice seeds by adopting the micro-nano bubbles and promoting growth of rice seedlings by adopting the micro-nano bubbles:
the rice seeds are germinated by adopting micro-nano bubbles, namely the rice seeds are soaked by adopting micro-nano bubble water, and the micro-nano bubble water is discharged after soaking is finished to perform static germination to obtain germinated rice seeds;
the method is characterized in that micro-nano bubbles are adopted to promote growth of rice seedlings, namely in the process of cultivating germinated rice seeds, micro-nano bubble processing is carried out on rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generating equipment according to certain frequency and cycle frequency, the processing frequency is 1-8 times every day, and the rice culture solution self-circulates for 6-10 times in the micro-bubble processing equipment in each processing process.
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) the method comprises the following steps of adopting micro-nano bubbles to accelerate germination of rice seeds:
firstly, using micro-nano bubble generating equipment to circularly treat tap water for 6-10 times to obtain micro-nano bubble water;
secondly, putting rice seeds into the container 1, adding the micro-nano bubble water obtained in the first step, sealing the container 1, and controlling the temperature to be 37 ℃ to soak for 24 hours;
the amount of the micro-nano bubble water used for soaking is based on that the micro-nano bubble water is immersed in the rice seeds and is 1.0cm higher than the surfaces of the rice seeds;
thirdly, after the soaking, discharging water in the container 1, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ and standing for 24 hours to obtain germinated rice seeds;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, then placing the rice seedling raising tray into a container 2 filled with a rice culture solution (the rice culture solution preferably adopts a commercially available MS rice culture solution), and controlling the rice culture solution to submerge the germinated rice seeds and be 0.1-0.5cm higher than the surface of the germinated rice seeds;
in each embodiment of the application, the rice culture solution is exemplified by using 1/8 times of the use concentration of the MS rice culture solution, but the application of other culture solutions suitable for rice culture in the application method of the micro-nano bubbles in rice seedling culture is not limited;
controlling the temperature of the container 2 to be 28 ℃ to perform culture for 10-21 days, preferably for 14 days, performing micro-nano bubble treatment on the rice culture solution in the container 2 in micro-nano bubble generation equipment according to a certain frequency and cycle frequency in the culture process, wherein the treatment frequency is 1-8 times per day, and performing self-circulation treatment on the rice culture solution in the micro-nano bubble generation equipment for 6-10 times in each treatment process to finally obtain rice seedlings.
Preferably, when the micro-nano bubble generation equipment is adopted to treat the rice culture solution in the container 2 for 1 time per day, namely the micro-nano bubble generation equipment is adopted to treat the rice culture solution 11:00 per day;
when the micro-nano bubble generating equipment is adopted to treat the rice culture solution in the container 2 for 4 times every day, namely the micro-nano bubble generating equipment is adopted to treat the rice culture solution at 6:00, 10:00, 14:00 and 18:00 every day;
when the micro-nano bubble generating equipment is adopted to treat the rice culture solution in the container 2 for 8 times every day, namely the micro-nano bubble generating equipment is adopted to treat the rice culture solution at 6:00, 8:00, 10:00, 12:00, 14:00, 16:00, 18:00 and 20:00 every day.
The micro-nano bubble generating devices used in the steps (1) and (2) are preferably produced by Shanghai Hengshi limited company, and only the micro-nano bubble generating devices produced by Shanghai Hengshi limited company and having a power of 100W and a working condition flow of 1.5L/min are exemplified in the specific embodiment of the application, but the application of the devices of other models is not limited, the selection of the specific model can be determined according to the quantity of rice seeds to be grown in batch treatment, the quantity of the rice seeds to be grown in batch treatment is large, the micro-nano bubble generating devices with relatively large power and relatively large working condition flow can be selected, the quantity of the rice seeds to be grown in batch treatment is small, and the micro-nano bubble generating devices with relatively small power and relatively small working condition flow can be selected.
According to the technical scheme, the rice seeds are germinated by the micro-nano bubbles, and the used micro-nano bubble water has the characteristics of increasing the dissolved oxygen content in the water body and having the characteristic of bubble particles, so that the rice seeds are soaked by the micro-nano bubble water, the rice seeds are convenient to germinate, and the rice seeds are strong in bud and high in germination rate;
meanwhile, the used nano bubble water is obtained after tap water is subjected to self-circulation treatment in the micro-nano bubble generating equipment for 6-10 times, and the dissolved oxygen content and the total number of micro-nano bubble particles in the obtained micro-nano bubble water can be obviously increased along with the increase of the circulation times of the tap water in the micro-nano bubble generating equipment, so that the rice seeds are soaked by the micro-nano bubble water obtained after the self-circulation treatment for 6-10 times, the germination of the rice seeds is promoted, and finally, the germination rate of the rice seeds germinated after being soaked by the micro-nano bubble water is improved by 10.93-11.74% compared with the germination rate of the rice seeds germinated after being soaked by common tap water in a comparative embodiment.
Furthermore, according to the technical scheme, the growth of the rice seedlings is promoted by adopting the micro-nano bubbles, and in the growth and cultivation stage of the rice seedlings, the rice culture solution is treated by adopting micro-nano bubble generation equipment every day, and particularly, the content of the plant growth hormone Gibberellin (GA) in the rice can be obviously increased after the treatment is carried out according to certain frequency and cycle times every day, so that the root length and the plant height of the obtained rice seedlings are obviously increased after the cultivation for 10-21 days, and the phenotypic state of rooting and strong seedlings is achieved.
Further, by adopting the technical scheme, namely, the growth of the rice seedlings is promoted by adopting the nano bubbles, micro-nano bubble processing is carried out on the rice culture solution by adopting micro-nano bubble generating equipment every day according to certain frequency and cycle frequency in the growth and cultivation stage of rice seedlings, and in each treatment process, the rice culture solution is subjected to self-circulation treatment for 6-10 times in the micro-nano bubble generation equipment, firstly, the treatment is carried out according to a certain frequency, can timely supplement water body dissolved oxygen and micro-nano bubble particles consumed in the growth process of the rice seedlings, thereby leading the germinated rice seeds to take root and strengthen the seedlings, further ensuring certain self-circulation treatment times in each treatment, thereby obviously increasing the dissolved oxygen content and the total number of micro-nano bubble particles in the obtained rice culture solution, and further strengthening the rooting and seedling strengthening of the germinated rice seeds.
Advantageous technical effects of the present application
According to the application method of the micro-nano bubbles in the rice seedling raising, the micro-nano bubbles are adopted to accelerate the germination of rice seeds and promote the growth of rice seedlings, so that the germination rate and the germination rate of the rice seeds are obviously improved, and the contents of technical indexes of Gibberellin (GA) change, such as GA1, GA3, GA4 and GA7, which are important plant growth hormones for rooting and strengthening of the rice are obviously increased (namely, the gibberellin is synthesized and the activity of the gibberellin is obviously increased in the rice), so that the application method of the micro-nano bubbles in the rice seedling raising is shown, under the synergistic effect that micro-nano bubble treatment with certain frequency and cycle times is carried out on rice culture solution every day by micro-nano bubble water for soaking the rice seeds and micro-nano bubble generation equipment, the root length and the plant height of the finally obtained rice seedlings are obviously increased, and achieves the phenotype state of promoting root growth and strengthening seedling.
Drawings
FIG. 1 is a phenotype diagram of the growth of rice seedlings obtained in comparative example, example 1 and example 2, wherein CK is comparative example, T1 is example 1, and T2 is example 2;
FIG. 2 is a histogram of the plant height and root length of young rice plants obtained in comparative example, example 1 and example 2, wherein a is a histogram of the plant height of young rice plants obtained in comparative example, example 1 and example 2, and b is a histogram of the root length of young rice plants obtained in comparative example, example 1 and example 2, wherein CK is comparative example, T1 is example 1, and T2 is example 2, and indicates in the graph, in comparison with comparative example,pa significant difference of < 0.01;
FIG. 3 is a histogram of the contents of the technical indices GA1, GA3, GA4, GA7 in the leaves and roots of young rice plants obtained in comparative example, example 1 and example 2, wherein a, b, c, d represent histograms of the contents of the technical indices GA1, GA3, GA4, GA7 in the leaves and roots of young rice plants obtained in comparative example, example 1 and example 2, respectively, wherein CK represents the comparative example, T1 represents example 1, T2 represents example 2, and a plot indicates, as compared to the comparative example,pa significant difference of < 0.01.
Detailed description of the invention
The technical solution of the present application is further illustrated by the following specific examples in combination with the accompanying drawings, but the present application is not limited thereto.
The rice culture solution used in each example and comparative example of the present application is a commercially available MS rice culture solution, and in the specific examples, 1/8-fold use concentration is adopted;
the micro-nano bubble generating equipment used in the embodiments of the application has the power of 100W and the working condition flow of 1.5L/min, and is produced by Shanghai Heng-Tech Co.
The method for determining the contents of GA1, GA3, GA4 and GA7 in the leaves or roots of the rice seedlings obtained in the examples of the application is a derivatization method, and the method is specifically shown in the following documents: chen, M.L., Fu, X.M., Liu, J.Q., Ye, T.T., Hou, S.Y., Huang, Y.Q., Yuan, B.F., Wu, Y.and Feng, Y.Q. (2012). Highlysensible and qualitative profiling of acidic physiological methods using differential amplification approach with respect to elevation acquisition coupled with nano-LC-ESI-Q-TOF-MS analysis, JChromatogr B.905, 67-74; an instrument used for determination is a Saimerfin high performance liquid chromatograph Ultimate 3000 combined with a triple quadrupole mass spectrometer TSQ Quantiva.
Comparative example
The method for raising rice seedlings by using tap water specifically comprises the following steps:
(1) carrying out germination accelerating treatment on rice seeds by using common tap water, and comprising the following specific processes:
putting rice seeds into a container 1, adding common tap water, sealing the container 1 for moisturizing, controlling the environmental temperature to be 37 ℃, and preserving heat for 24 hours;
the amount of the tap water used for soaking is based on that the tap water is immersed in the rice seeds and is 1.0cm higher than the surface of the rice seeds;
discharging water in the container 1 after soaking, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ for germination for 24 hours to obtain germinated rice seeds;
through determination, the germination rate of the rice seeds is 85.76%;
(2) the method comprises the following steps of:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.2cm higher than the surface of the germinated rice seeds;
② controlling the temperature of the container 2 to be 28 ℃ to culture for 14 days, and finally obtaining the rice seedling.
Example 1
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) the method adopts micro-nano bubbles to accelerate germination of rice seeds, and comprises the following specific processes:
firstly, self-circulation treatment is carried out on tap water for 8 times by using micro-nano bubble generating equipment to obtain micro-nano bubble water;
secondly, putting rice seeds into the container 1, adding the micro-nano bubble water obtained in the first step, sealing the container 1, moisturizing, controlling the environmental temperature to be 37 ℃, and preserving heat for 24 hours;
the amount of the micro-nano bubble water used for soaking is based on that the micro-nano bubble water is immersed in the rice seeds and is 1.0cm higher than the surfaces of the rice seeds;
thirdly, after the soaking, discharging water in the container 1, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ for germinating for 24 hours to obtain germinated rice seeds;
through determination, the germination rate of the rice seeds is 95.14 percent;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.2cm higher than the surface of the germinated rice seeds;
secondly, controlling the temperature of the container 2 to be 28 ℃ for 14 days, and performing micro-nano bubble treatment on the rice culture solution in the container 2 for 1 time every day by adopting micro-nano bubble generation equipment in the culture process to finally obtain rice seedlings;
the micro-nano bubbles are processed for 1 time every day, namely, the micro-nano bubble generating equipment is used for processing every 11:00, and the rice culture solution in the container 2 is subjected to self-circulation processing for 8 times in the micro-nano bubble generating equipment in the processing process.
Example 2
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) accelerating germination of the rice seeds by adopting the micro-nano bubbles, wherein the specific process is the same as the step (1) of the embodiment 1, so that the germinated rice seeds are obtained;
through determination, the germination rate of the rice seeds is 95.83 percent;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.2cm higher than the surface of the germinated rice seeds;
controlling the temperature of the container 2 to be 28 ℃ for 14 days, and treating the rice culture solution in the container 2 for 8 times every day by adopting micro-nano bubble generation equipment in the culture process to finally obtain rice seedlings;
the micro-nano bubbles are processed for 8 times every day, namely, the micro-nano bubbles are processed by adopting micro-nano bubble generating equipment at 6:00, 8:00, 10:00, 12:00, 14:00, 16:00, 18:00 and 20:00 every day, and the rice culture solution in the container 2 is subjected to self-circulation processing for 8 times in the micro-nano bubble generating equipment in each processing process.
The germination rates of the rice seeds obtained in the step (1) of the rice seedling raising methods of the comparative example, the example 1 and the example 2 are 85.76%, 95.14% and 95.83%, respectively, so that it can be seen that the germination rate of the rice seeds soaked in the micro-nano bubble water is increased by 10.93-11.74% compared with that of the rice seeds soaked in the common tap water and then germinated in the comparative example, which indicates that the method is more favorable for promoting the germination of the seeds after the rice seeds are soaked in the micro-nano bubble water.
The growth phenotype graphs of the rice seedlings obtained in the comparison example, the example 1 and the example 2 are shown in fig. 1, and as can be seen from fig. 1, the rice seeds are subjected to pregermination by the micro-nano bubbles and the rice seedlings are subjected to growth promotion by the micro-nano bubbles, and the finally obtained growth state of the rice seedlings is obviously superior to that of the comparison example.
Further, quantitative data analysis is carried out on the plant height and the root length of the rice seedlings obtained in the comparison example, the example 1 and the example 2, a histogram of the plant height and the root length is shown in fig. 2, wherein CK represents the rice seedling obtained in the comparison example, T1 represents the rice seedling obtained in the example 1, and T2 represents the rice seedling obtained in the example 2, as can be seen from fig. 2, the method adopts the micro-nano bubbles to accelerate germination of the rice seed and the micro-nano bubbles to accelerate growth of the rice seedling, and finally the plant height of the obtained rice seedling is obviously increased, and the root length is also obviously increased.
Further, the technical indicators GA1, GA3, GA4 and GA7 in the leaves and roots of the rice seedlings obtained in the above comparative examples, examples 1 and 2, which characterize the content of the plant growth hormone Gibberellin (GA), are respectively measured, and the results are shown in fig. 3, and it can be seen from fig. 3 that, compared with the comparative examples, in examples 1 and 2 of the present application, because the micro-nano bubbles are used for accelerating germination of the rice seeds and the micro-nano bubbles are used for promoting growth of the rice seedlings, the final technical indicators GA1, GA3, GA4 and GA7 in the leaves and roots of the obtained rice seedlings, which characterize the content of the gibberellin, are all significantly increased, thereby indicating that the content of the plant growth hormone gibberellin in the rice seedlings is increased, the growth is activated, and the effect of rooting and seedling growth is achieved. The reason for the analysis may be that the rice seeds are soaked by micro-nano bubble water in the germination accelerating stage of the rice seeds so as to promote the rapid germination of the rice seeds, the micro-nano bubble generation equipment is used for processing the rice culture solution at a certain frequency and cycle times in the growth and cultivation stage of the rice seedlings, so that the contents of the technical indexes GA1, GA3, GA4 and GA7 which characterize the gibberellin content in the leaves and roots of the rice seedlings can be improved, particularly the increase of the processing times is more remarkable, and the contents of the technical indexes GA1, GA3, GA4 and GA7 which characterize the gibberellin content are increased. Meanwhile, the rapid germination of rice seeds and the increase of the content of the technical indexes GA1, GA3, GA4 and GA7 for representing the gibberellin content can also be the result of the synergistic effect after the micro-nano bubbles are treated at different stages, namely the synergistic effect result after the micro-nano bubbles are treated at different stages, so that the rapid growth of rice seedlings is promoted, and the technical effect of rooting and strengthening is further achieved.
Example 3
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) the method adopts micro-nano bubbles to accelerate germination of rice seeds, and comprises the following specific processes:
firstly, self-circulation treatment is carried out on tap water for 6 times by using micro-nano bubble generating equipment to obtain micro-nano bubble water;
secondly, putting rice seeds into the container 1, adding the micro-nano bubble water obtained in the first step, sealing the container 1, moisturizing, controlling the environmental temperature to be 37 ℃, and preserving heat for 24 hours;
the amount of the micro-nano bubble water used for soaking is based on that the micro-nano bubble water is immersed in the rice seeds and is 1.0cm higher than the surfaces of the rice seeds;
thirdly, after the soaking, discharging water in the container 1, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ for germinating for 24 hours to obtain germinated rice seeds;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.1cm higher than the surface of the germinated rice seeds;
secondly, controlling the temperature of the container 2 to be 28 ℃ for culturing for 10 days, and treating the rice culture solution in the container 2 for 8 times every day by adopting micro-nano bubble generation equipment in the culturing process to finally obtain rice seedlings;
the micro-nano bubbles are processed for 8 times every day, namely, the micro-nano bubbles are processed by adopting micro-nano bubble generating equipment at 6:00, 8:00, 10:00, 12:00, 14:00, 16:00, 18:00 and 20:00 every day, and the rice culture solution in the container 2 is subjected to self-circulation processing for 6 times in the micro-nano bubble generating equipment in each processing process.
Example 4
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) the method adopts micro-nano bubbles to accelerate germination of rice seeds, and comprises the following specific processes:
firstly, self-circulation treatment is carried out on tap water for 10 times by using micro-nano bubble generating equipment to obtain micro-nano bubble water;
secondly, putting rice seeds into the container 1, adding the micro-nano bubble water obtained in the first step, sealing the container 1, moisturizing, controlling the environmental temperature to be 37 ℃, and preserving heat for 24 hours;
the amount of the micro-nano bubble water used for soaking is based on that the micro-nano bubble water is immersed in the rice seeds and is 1.0cm higher than the surfaces of the rice seeds;
thirdly, after the soaking, discharging water in the container 1, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ for germinating for 24 hours to obtain germinated rice seeds;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.5cm higher than the surface of the germinated rice seeds;
secondly, controlling the temperature of the container 2 to be 28 ℃ for culturing for 21 days, and treating the rice culture solution in the container 2 for 8 times every day by adopting micro-nano bubble generation equipment in the culturing process to finally obtain rice seedlings;
the micro-nano bubbles are processed for 8 times every day, namely, the micro-nano bubbles are processed by adopting micro-nano bubble generating equipment at 6:00, 8:00, 10:00, 12:00, 14:00, 16:00, 18:00 and 20:00 every day, and the rice culture solution in the container 2 is subjected to self-circulation processing for 10 times in the micro-nano bubble generating equipment in each processing process.
Example 5
The application method of the micro-nano bubbles in rice seedling raising specifically comprises the following steps:
(1) the method adopts micro-nano bubbles to accelerate germination of rice seeds, and comprises the following specific processes:
firstly, self-circulation treatment is carried out on tap water for 10 times by using micro-nano bubble generating equipment to obtain micro-nano bubble water;
secondly, putting rice seeds into the container 1, adding the micro-nano bubble water obtained in the first step, sealing the container 1, moisturizing, controlling the environmental temperature to be 37 ℃, and preserving heat for 24 hours;
the amount of the micro-nano bubble water used for soaking is based on that the micro-nano bubble water is immersed in the rice seeds and is 1.0cm higher than the surfaces of the rice seeds;
thirdly, after the soaking, discharging water in the container 1, then sealing the container 1 again, and continuously controlling the temperature to be 37 ℃ for germinating for 24 hours to obtain germinated rice seeds;
(2) adopting micro-nano bubbles to promote the growth of rice seedlings, and comprising the following steps:
putting the germinated rice seeds obtained in the step (1) into a rice seedling raising tray, and then placing the rice seedling raising tray into a container 2 filled with a rice culture solution;
the amount of the rice culture solution in the container 2 is determined according to that the rice culture solution is immersed in the germinated rice seeds and is 0.5cm higher than the surface of the germinated rice seeds;
controlling the temperature of the container 2 to be 28 ℃ for culturing for 21 days, and treating the rice culture solution in the container 2 for 4 times every day by adopting micro-nano bubble generation equipment in the culturing process to finally obtain rice seedlings;
the micro-nano bubbles are processed for 4 times every day, namely, the micro-nano bubble generation equipment is used for processing at 6:00, 10:00, 14:00 and 18:00 every day, and the rice culture solution in the container 2 is subjected to self-circulation processing for 10 times in the micro-nano bubble generation equipment in each processing process.
In summary, according to the application method of the micro-nano bubbles in the rice seedling raising, due to the synergistic effect of the application of the micro-nano bubbles, the rice seeds can rapidly germinate and the rice seedlings can rapidly grow, so that the rooting and the seedling strengthening of the rice seeds are promoted, and the rice seedlings with strong roots and strong seedlings are finally obtained.
The above-mentioned embodiments are merely illustrative and not restrictive, and those skilled in the art can make various modifications as required after reading the description, but fall within the scope of the appended claims.
Claims (10)
1. The application method of the micro-nano bubbles in rice seedling raising is characterized by comprising the two steps of accelerating germination of rice seeds by adopting the micro-nano bubbles and promoting growth of rice seedlings by adopting the micro-nano bubbles:
the rice seeds are germinated by adopting micro-nano bubbles, namely the rice seeds are soaked by adopting micro-nano bubble water, and the micro-nano bubble water is discharged after soaking is finished, and standing and sprouting are carried out to obtain germinated rice seeds;
the method is characterized in that micro-nano bubbles are adopted to promote growth of rice seedlings, namely, in the process of cultivating the germinated rice seeds, micro-nano bubble processing is carried out on rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generating equipment according to certain frequency and cycle times.
2. The method for applying micro-nano bubbles in rice seedling culture according to claim 1, wherein the micro-nano bubble water for germination acceleration is obtained by performing self-circulation treatment on tap water in micro-nano bubble generation equipment for 6-10 times by adopting micro-nano bubble generation equipment.
3. The method for applying micro-nano bubbles in rice seedling raising according to claim 1, wherein the amount of micro-nano bubble water used for soaking the germinated rice seeds is based on that the micro-nano bubble water can immerse the rice seeds and is 0.1-0.5cm higher than the surface of the rice seeds.
4. The method of claim 1, wherein the micro-nano bubbles are treated with the rice culture solution used for cultivating the germinated rice seeds by the micro-nano bubble generating device according to a certain frequency and cycle number, that is, the treatment frequency of the rice culture solution used for cultivating the germinated rice seeds by the micro-nano bubble generating device is 1-8 times per day, and the rice culture solution is self-circulated in the micro-bubble treating device for 6-10 times in each treatment process.
5. The method for applying micro-nano bubbles in rice seedling culture according to claim 4, wherein the micro-nano bubble treatment is performed on the rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generation equipment according to a certain frequency and cycle times, namely the frequency of the treatment on the rice culture solution used for cultivating the germinated rice seeds by adopting the micro-nano bubble generation equipment every day is 1-4 times.
6. The method for applying micro-nano bubbles in rice seedling culture according to claim 4, wherein the micro-nano bubble treatment is performed on the rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generation equipment according to a certain frequency and cycle times, namely the frequency of the treatment on the rice culture solution used for cultivating the germinated rice seeds by adopting the micro-nano bubble generation equipment every day is 4-8 times.
7. The method for applying micro-nano bubbles in rice seedling culture according to claim 4, wherein the micro-nano bubble treatment is performed on the rice culture solution used for cultivating the germinated rice seeds by adopting the micro-nano bubble generation equipment according to a certain frequency and cycle times, namely the frequency of the treatment on the rice culture solution used for cultivating the germinated rice seeds by adopting the micro-nano bubble generation equipment every day is 8 times.
8. The method for applying micro-nano bubbles in rice seedling raising according to claim 7, wherein the frequency of treating the rice culture solution used for the cultivation by adopting the micro-nano bubble generating equipment every day is 8 times, namely the micro-nano bubble generating equipment is respectively used for treating at 6:00, 8:00, 10:00, 12:00, 14:00, 16:00, 18:00 and 20:00 every day.
9. The application method of the micro-nano bubbles in rice seedling raising according to any one of claims 1 to 8, wherein the rice culture solution is MS rice culture solution.
10. The application method of the micro-nano bubbles in rice seedling culture according to any one of claims 1 to 8, which is characterized by comprising the following steps:
the rice seed germination accelerating method comprises the following steps of (1) accelerating germination of rice seeds by adopting micro-nano bubbles: soaking rice for 24 hours at 37 ℃ by adopting micro-nano bubble water, then discharging the micro-nano bubble water, and standing for 24 hours under a closed condition and continuously controlling the temperature to be 37 ℃ to obtain germinated rice seeds;
the growth of the rice seedlings is promoted by adopting micro-nano bubbles: putting the germinated rice seeds into a rice seedling raising tray, then placing the rice seedling raising tray into a container filled with a rice culture solution, controlling the temperature to be 28 ℃ for 10-21 days after the rice culture solution is immersed in the germinated rice seeds and is 0.1-0.5cm higher than the surface of the germinated rice seeds, wherein the treatment frequency of the rice culture solution used for cultivating the germinated rice seeds by adopting micro-nano bubble generation equipment every day in the cultivation process is 1-8 times, and the rice culture solution performs self-circulation in micro-bubble treatment equipment for 6-10 times in each treatment process, so that the rice seedlings are finally obtained.
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