CN111743152A - Health food for inhibiting tumor and improving immunity and preparation method thereof - Google Patents
Health food for inhibiting tumor and improving immunity and preparation method thereof Download PDFInfo
- Publication number
- CN111743152A CN111743152A CN202010769863.0A CN202010769863A CN111743152A CN 111743152 A CN111743152 A CN 111743152A CN 202010769863 A CN202010769863 A CN 202010769863A CN 111743152 A CN111743152 A CN 111743152A
- Authority
- CN
- China
- Prior art keywords
- parts
- powder
- health food
- tumor
- prepared
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 40
- 235000013402 health food Nutrition 0.000 title claims abstract description 30
- 230000036039 immunity Effects 0.000 title claims abstract description 30
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 65
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 25
- 239000011669 selenium Substances 0.000 claims abstract description 25
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims abstract description 20
- 241001264174 Cordyceps militaris Species 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 12
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 12
- 241000018646 Pinus brutia Species 0.000 claims abstract description 12
- UEDUENGHJMELGK-HYDKPPNVSA-N Stevioside Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UEDUENGHJMELGK-HYDKPPNVSA-N 0.000 claims abstract description 11
- 229940013618 stevioside Drugs 0.000 claims abstract description 11
- OHHNJQXIOPOJSC-UHFFFAOYSA-N stevioside Natural products CC1(CCCC2(C)C3(C)CCC4(CC3(CCC12C)CC4=C)OC5OC(CO)C(O)C(O)C5OC6OC(CO)C(O)C(O)C6O)C(=O)OC7OC(CO)C(O)C(O)C7O OHHNJQXIOPOJSC-UHFFFAOYSA-N 0.000 claims abstract description 11
- 235000019202 steviosides Nutrition 0.000 claims abstract description 11
- 235000012055 fruits and vegetables Nutrition 0.000 claims abstract description 8
- 235000013339 cereals Nutrition 0.000 claims abstract description 7
- 241000195493 Cryptophyta Species 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 9
- 230000002708 enhancing effect Effects 0.000 claims description 9
- 235000000832 Ayote Nutrition 0.000 claims description 8
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 8
- 240000001980 Cucurbita pepo Species 0.000 claims description 8
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 8
- 244000000626 Daucus carota Species 0.000 claims description 8
- 235000002767 Daucus carota Nutrition 0.000 claims description 8
- 235000015136 pumpkin Nutrition 0.000 claims description 8
- 235000013312 flour Nutrition 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 208000032791 BCR-ABL1 positive chronic myelogenous leukemia Diseases 0.000 claims description 6
- 206010006187 Breast cancer Diseases 0.000 claims description 6
- 208000026310 Breast neoplasm Diseases 0.000 claims description 6
- 208000010833 Chronic myeloid leukaemia Diseases 0.000 claims description 6
- 206010058467 Lung neoplasm malignant Diseases 0.000 claims description 6
- 208000033761 Myelogenous Chronic BCR-ABL Positive Leukemia Diseases 0.000 claims description 6
- 201000005202 lung cancer Diseases 0.000 claims description 6
- 208000020816 lung neoplasm Diseases 0.000 claims description 6
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 claims description 5
- 241000195620 Euglena Species 0.000 claims description 5
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 claims description 5
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 claims description 5
- 150000004676 glycans Chemical class 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 229920002498 Beta-glucan Polymers 0.000 claims description 4
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000010902 jet-milling Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000010298 pulverizing process Methods 0.000 claims description 3
- 235000005911 diet Nutrition 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 238000002560 therapeutic procedure Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 230000037213 diet Effects 0.000 claims 1
- 210000004881 tumor cell Anatomy 0.000 abstract description 9
- 230000004663 cell proliferation Effects 0.000 abstract description 4
- 230000001093 anti-cancer Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 238000002512 chemotherapy Methods 0.000 abstract 1
- 230000009977 dual effect Effects 0.000 abstract 1
- 238000001959 radiotherapy Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 57
- 230000000694 effects Effects 0.000 description 16
- 230000005764 inhibitory process Effects 0.000 description 14
- 239000000047 product Substances 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 10
- 201000011510 cancer Diseases 0.000 description 8
- 210000003734 kidney Anatomy 0.000 description 7
- 210000004072 lung Anatomy 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 238000009472 formulation Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000190633 Cordyceps Species 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 2
- 108010087230 Sincalide Proteins 0.000 description 2
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 238000010609 cell counting kit-8 assay Methods 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 208000013116 chronic cough Diseases 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012091 fetal bovine serum Substances 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011127 radiochemotherapy Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000005919 time-dependent effect Effects 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 238000012404 In vitro experiment Methods 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010041497 Spermatorrhoea Diseases 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 208000023819 chronic asthma Diseases 0.000 description 1
- 230000002559 cytogenic effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000235 effect on cancer Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/04—Sulfur, selenium or tellurium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/02—Algae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/066—Clavicipitaceae
- A61K36/068—Cordyceps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A61P35/02—Antineoplastic agents specific for leukemia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A health food for inhibiting tumor and improving immunity and a preparation method thereof are provided, the health food is prepared from the following raw materials in parts by weight: 13-25 parts of cordyceps militaris powder, 6-15 parts of pine pollen, 5-15 parts of selenium-rich malt powder, 3-12 parts of naked algae powder, 20-70 parts of fruit and vegetable coarse cereal powder and 0-5 parts of stevioside. The health food has dual anticancer effects, can inhibit tumor cell proliferation, and improve immunity, and is a nutritional partner for tumor patients during operation, radiotherapy, chemotherapy and rehabilitation.
Description
Technical Field
The invention provides a health food for inhibiting tumor and improving immunity, belonging to the technical field of health foods.
Background
The number of new cancer cases is about 380 million each year, the number of deaths is about 229 million, the average annual incidence rate of the cancer is increased by about 3.9 percent, and the incidence rate and the mortality rate are on the increasing trend year by year. Cancer becomes one of four chronic diseases in China, and the health of people in China is seriously influenced.
Aiming at the treatment of cancer, China advocates multidisciplinary and standardized comprehensive treatment. In the comprehensive treatment of tumors, the traditional Chinese medicine not only assists the treatment of western medicine operations and chemoradiotherapy, but also can dialectically treat different cancer species at different stages.
At present, some cordyceps products and selenium-rich products are used for the auxiliary treatment of cancer prevention and cancer resistance, but the curative effect of the cordyceps products and the selenium-rich products is still to be further improved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides the health food with the effects of inhibiting tumor and improving immunity, has double anti-cancer effects, can inhibit the proliferation of tumor cells and improve the immunity of the organism, and is a nutrition partner of tumor patients during operations, chemoradiotherapy and rehabilitation.
In order to achieve the purpose, the invention comprises the following technical scheme:
a health food for inhibiting tumor and improving immunity is prepared from the following raw materials in parts by weight: 13-25 parts of cordyceps militaris powder, 6-15 parts of pine pollen, 5-15 parts of selenium-rich malt powder, 3-12 parts of naked algae powder, 20-70 parts of fruit and vegetable coarse cereal powder and 0-5 parts of stevioside.
The health food for inhibiting tumor and improving immunity is preferably prepared from the following raw materials in parts by weight: 17-20 parts of cordyceps militaris powder, 10-12 parts of pine pollen, 9-11 parts of selenium-rich malt powder, 6-8 parts of euglena powder, 50-60 parts of fruit and vegetable coarse cereal powder and 0-2 parts of stevioside.
The health food for inhibiting tumor and improving immunity as described above is preferably prepared from the cordyceps militaris powder by the following method: the artificially cultured fresh cordyceps militaris sporocarp is prepared by drying, crushing and sieving processes, wherein the content of cordycepin is 1 wt%.
The health food for inhibiting tumor and improving immunity is preferably prepared from the following raw materials by the following steps: manually collected pollen pini is sieved by a 120-mesh sieve, and the pollen pini is subjected to jet milling to break the wall, wherein the content of crude polysaccharide is 1.5 wt%. Preferably, the frequency of the wall breaking machine is 55 Hz-60 Hz, and the pressure of the air source is 0.7-1.0 MPa.
The health food for inhibiting tumor and improving immunity is preferably prepared from selenium-rich malt sprouts by cleaning, drying and pulverizing, wherein the selenium content is 30-50 mg/kg.
Preferably, the gymnocyanine powder is prepared by culturing, concentrating, drying and sieving gymnocyanine seeds, and the content of beta-glucan is 50 wt%.
Preferably, the fruit, vegetable and coarse cereal powder is prepared from one or more of orange powder, pumpkin powder, carrot powder and malt powder, and the fruit and vegetable powder is an aqueous extract of corresponding fruits and vegetables.
In another aspect, the present invention provides a method for preparing a health food for inhibiting tumor and enhancing immunity, the method comprising the steps of: weighing the raw materials, uniformly mixing, and filling to obtain the product.
In another aspect, the invention provides the application of the health food for inhibiting tumor and improving immunity as a dietary therapy product for inhibiting tumor and improving immunity, wherein the oral dose is 5-10 g/day.
Preferably, the tumor is human lung cancer cell A549, human breast cancer cell MCF-7 or human chronic myelogenous leukemia cell K562.
The invention selects high-quality raw materials which are natural and higher than natural, provides the health food with the tumor cell inhibition effect and the preparation method thereof, and has reliable and safe effect and good taste.
The traditional Chinese medicine considers that the cordyceps sinensis enters the lung and kidney meridians, can tonify lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, soreness and pain of waist and knees, weakness after illness, chronic cough and weakness, cough and phlegmatic blood, spontaneous perspiration and night sweat and the like, and is the only traditional Chinese medicine capable of balancing and adjusting yin and yang. Record of Chinese medicine sea: cordyceps militaris, also known as Cordyceps militaris, is sweet and neutral in nature and flavor and enters lung and kidney meridians. The product has effects of invigorating kidney yang, nourishing lung yin, protecting lung, invigorating kidney, replenishing essence, and invigorating qi, and has good therapeutic effect on lung and kidney deficiency, chronic cough, asthma, and cough with excessive phlegm and blood. The Chinese herbal medicine compilation records: the fruiting body and polypide of Cordyceps militaris can also be used as a substitute for Cordyceps.
Pollen Pini is a kind of product which can be used as both medicine and food and is collected in ancient Chinese medical books. Li Shizhen is recorded in Ben Cao gang mu: pine pollen, sweet, warm and nontoxic; moisten heart and lung, tonify qi, dispel wind to stop bleeding. Pollen pini contains more than 200 kinds of nutrients such as saccharides, lipids, proteins, amino acids, nucleic acids, vitamins, minerals, etc., including 8 kinds of amino acids, 14 kinds of vitamins and 24 kinds of trace elements essential for human body, and is known as "micro-nutrient bank" and has multiple functions of regulating immunity, resisting fatigue, improving gastrointestinal function, regulating blood fat and protecting liver.
Selenium is a trace element necessary for human body, cannot be synthesized in human body, is an element easy to excrete, and gradually decreases with age. Researches find that selenium is closely related to the occurrence and development of tumors. Selenium can help to remove free radicals in vivo, repair membrane molecule damage, and prevent genetic variation or canceration of organism cells; selenium has an activating effect on a DNA repair system and can resist the damage of a carcinogen to DNA; selenium can induce tumor cell apoptosis.
The Euglena contains more than 20% of protein, essential vitamins, minerals, amino acids, unsaturated fatty acids, etc., is natural and comprehensive nutrient substance, is rich in beta (1, 3) glucan, and can stimulate non-specific immunity of human body.
The health food of the invention compounds each active component according to specific proportion, wherein: cordycepin, Cordyceps polysaccharide and adenosine in Cordyceps militaris have anti-tumor effect; selenium is a strong antioxidant and can assist in clearing free radicals in vivo and prevent cytogenetic variation; the compound of the euglena and the pine pollen can supplement natural and comprehensive nutrient substances and can stimulate the autoimmune system of a human body.
The invention has the beneficial effects that: the invention selects high-quality raw materials to form the health food for inhibiting tumor and improving immunity, the components are mutually matched and synergically, and the inhibition effect on cancer cells is obviously higher than that of a single component. Experimental results show that the health food has the effect of inhibiting human lung cancer cells A549, human breast cancer cells MCF-7 and human chronic myelogenous leukemia cells K562, and has reliable and safe effect and good taste.
Drawings
FIG. 1 is a photograph of the morphology of A549 cells treated for 48h with sample 1 and control group.
FIG. 2 is a photograph of the morphology of MCF-7 cells treated for 48 hours in sample 1 and control groups.
FIG. 3 is a photograph of the morphology of K562 cells treated with sample 1 and control group for 48 h.
Detailed Description
Sources of some of the raw materials in the following examples:
the cordyceps militaris powder is purchased from the development center of Shanghai national treasure enterprises, the content of the cordycepin is 1 percent, and the product is prepared by adopting the following method: the artificially cultured fresh cordyceps militaris sporocarp is prepared by drying, crushing and sieving processes.
The pine pollen is prepared by the following method: sieving manually collected pollen pini with a 120-mesh sieve, performing jet milling to break the walls, wherein the frequency of a wall breaking machine is 55 Hz-60 Hz, the pressure of an air source is 0.7-1.0 MPa, and the content of crude polysaccharide is 1.5%.
The selenium-rich malt flour is purchased from Tianjin Tianma Kangbi selenium technology limited company, the selenium content is 30mg/kg, and the product is prepared by the following method: the selenium-rich wheat sprout is prepared by cleaning, drying and pulverizing.
The gymnocyanin powder is purchased from the American Kemin company (KEMIN), the content of beta-glucan is 50%, and the product is prepared by the following method: the euglena seeds are prepared by culturing, concentrating, drying and sieving.
Example 1 health food for suppressing tumor and enhancing immunity
1. Formulation of
15 parts of cordyceps militaris powder, 13 parts of pine pollen, 13 parts of selenium-rich malt powder, 8 parts of naked algae powder, 12 parts of pumpkin powder, 12 parts of carrot powder, 12 parts of orange powder, 12 parts of malt powder and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a sample 1.
Example 2 health food for inhibiting tumor and enhancing immunity
1. Formulation of
20 parts of cordyceps militaris powder, 13 parts of pine pollen, 13 parts of selenium-rich malt powder, 8 parts of naked algae powder, 12 parts of pumpkin powder, 12 parts of carrot powder, 12 parts of orange powder, 12 parts of malt powder and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a sample 2.
Comparative example 1
1. Formulation of
49 parts of cordyceps militaris powder, 12 parts of pumpkin powder, 12 parts of carrot powder, 12 parts of orange powder, 12 parts of malt powder and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 1.
Comparative example 2
1. Formulation of
49 parts of pine pollen, 12 parts of pumpkin powder, 12 parts of carrot powder, 12 parts of orange powder, 12 parts of malt powder and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 2.
Comparative example 3
1. Formulation of
49 parts of selenium-rich malt flour, 12 parts of pumpkin flour, 12 parts of carrot flour, 12 parts of orange flour, 12 parts of malt flour and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 3.
Comparative example 4
1. Formulation of
49 parts of naked algae powder, 12 parts of pumpkin powder, 12 parts of carrot powder, 12 parts of orange powder, 12 parts of malt powder and 1 part of stevioside.
2. Preparation method
The raw materials are uniformly mixed and filled to obtain a comparative sample 4.
Experimental example 1
The samples 1 to 2 and the comparative samples 1 to 4 prepared in the examples and comparative examples were used to examine the inhibitory effects of these samples on human lung cancer cells A549, human breast cancer cells MCF-7 and human chronic myelogenous leukemia cells K562.
1. Principal materials and instruments
Human lung cancer cell A549, human breast cancer cell MCF-7 and human chronic myelogenous leukemia cell K562 were purchased from Bai Biotech Co., Ltd, Kyoto.
DMEM serum-free cell culture medium was purchased from Gibco; fetal Bovine Serum (FBS) and 100 Xpenicillin streptomycin mixed liquor are purchased from Hyclone company; trypsin was purchased from Roche; DMSO was purchased from Solarbio corporation; CCK-8 is available from Donjindo; PBS (phosphate buffered saline) was purchased from BBI life science; carbon dioxide incubators were purchased from Panasonic corporation; inverted optical microscope and fluorescence microscope were purchased from Nikon, Japan; desktop refrigerated centrifuges were purchased from HITACHI corporation; stat Fax-2100 enzyme linked immunosorbent assay (ELISA) instrument available from BIO-RAD; t25 cell culture flasks were purchased from Fisher; model HH-4 digital display thermostat water bath from Thermo corporation; YDS-20 type liquid nitrogen biological containers are purchased from Chengdu Jinfeng liquid nitrogen container GmbH; clean bench (SW-CJ-2D type) was purchased from NUAIRE corporation.
2. Experimental methods
2.1 tumor cell inhibition assay
A549, MCF-7 and K562 cells in logarithmic growth phase were selected as 1 × 105Each/mL was inoculated in a 96-well plate at 100. mu.L of DMEM medium per well. After the cells are cultured for 12h, adding 100 mu L (200 mu g/mL) of samples, and arranging 6 samples in parallel; the negative control group is that the cells are added with culture solution with the same volume; the blank group had only culture medium; standing at 37 deg.C for 5% CO2And incubating for 12h, 24h and 48h in an incubator with saturated humidity. After the culture is finished, carefully sucking out the original culture solution, adding 10 mu L of CCK-8 solution into each well, incubating for 1-4h, and then measuring the light absorption value under the wavelength of 450nm by using an enzyme-labeling instrument. The inhibition rate of the sample on the cells is calculated according to the following formula:
inhibition (%) (experimental OD value-negative OD value)/(negative OD value-blank OD value) × 100%
2.2 morphological Observation of tumor cells
A549, MCF-7 and K562 cells in logarithmic growth phase were selected as 1 × 105one/mL was inoculated in 12-well plates, 1mL DMEM medium per well. After 12h of cell culture, sample 1 was added, 1mL per well (200. mu.g/mL); the control group is cells added with culture solution with the same volume; standing at 37 deg.C for 5% CO2And incubating for 48h in an incubator with saturated humidity. After the culture was completed, the original culture solution was carefully aspirated, the cells were gently washed with PBS 2 times, and then stained with a certain volume of trypan blue, followed by observation with a fluorescent microscope. For K562 suspension cells, a certain volume of culture solution is directly sucked, centrifuged at 1000rpm for 5 minutes, washed once with PBS, centrifuged, added with a certain volume of culture medium, gently blown and uniformly mixed, added with trypan blue with the same volume for dyeing, and observed by a fluorescence microscope.
2.3 data analysis
Data were statistically analyzed using prism 8.0.
3. Results of the experiment
3.1 Effect of samples on the growth of human Lung cancer cells A549
A549 cells show different tolerance after the samples are acted for 12, 24 and 48 hours. The results are shown in table 1:
wherein, the sample 1 treats the A549 cells for 12h, and the sample promotes the cell proliferation of the A549 cells to reach 19.4%. After the A549 cells are treated for 24 hours, the sample 1 inhibits the proliferation of the A549 cells, and the inhibition rate reaches 22.3%. After 48 hours of treatment, the inhibition rate of the sample 1 on the proliferation of the A549 cells reaches 48.6 percent.
The results show that the inhibition effect of the sample 1 and the sample 2 on the A549 cells shows different effects along with different time, and the cordyceps sinensis polysaccharide has certain effect of promoting cell proliferation after being treated for 12 hours, and possibly has certain protection effect on the cells; with the increase of the treatment time, the cordycepin begins to exert the inhibition effect on the cell growth.
TABLE 1
3.2 Effect of samples on the growth of human Breast cancer cells MCF-7
MCF-7 cells show different tolerance and inhibition effects after sample action for 12, 24 and 48 hours. The results are shown in table 2:
among them, samples 1 and 2 showed promotion-then-inhibition of MCF-7 cell proliferation, probably for the same reason as a549 cells. The highest inhibition rate of sample 1 at 48h reaches 13.4%.
TABLE 2
3.3 Effect of samples on the growth of Chronic myelogenous leukemia cells K562
The K562 cell strain shows different tolerance and shows time-dependent effect after samples are treated for 12, 24 and 48 hours. The results are shown in Table 3:
among them, sample 1 had the best inhibitory effect, and after 12h of treatment of K562 cells, the inhibitory rate of sample 1 was 26.8%. After K562 cells are treated for 24 hours, the inhibition rate reaches 66.0%. After K562 cells were treated for 48h, the cell inhibition rate of sample 1 treatment continued to increase to 80.5%.
TABLE 3
3.4 morphological Observation of samples after treatment of different cells
FIG. 1 is a photograph showing the morphology of A549 cells treated for 48 hours in sample 1 and control group, wherein A549 cells treated for 48 hours in sample are observed by a fluorescence microscope after staining. As can be seen, some debris appeared in the group of sample 1, i.e., the cell debris after cell disruption, most of the cells treated with sample 1 were disrupted, and the cells in the control group were intact.
FIG. 2 is a photograph showing the morphology of MCF-7 cells treated for 48 hours in sample 1 and control group, and the results of fluorescence microscopy show that most of the cells in the control group are intact, and the cell membrane of the sample 1 treated group is ruptured, and the content flows out.
FIG. 3 is a photograph showing the morphology of K562 cells after 48 hours of treatment in sample 1 and control group, and most of the cells in the treatment group are ruptured and the morphology of cell membranes cannot be distinguished after K562 cells are treated in sample 1 for 48 hours.
4. Conclusion
From the results of in vitro experiments, the optimal inhibition rates of the sample on A549, MCF-7 and K562 tumor cells are 48.6%, 13.4% and 80.5%, respectively. In a certain concentration range, the growth inhibition of tumor cells by samples 1 and 2 showed time-dependent effect. The specificity of the sample on the growth inhibition of tumor cells may be caused by different cell morphological structures on one hand and different cell signaling pathway regulation of three kinds of cancer cells on the other hand.
Claims (10)
1. A health food for inhibiting tumor and improving immunity is characterized by being prepared from the following raw materials in parts by weight: 13-25 parts of cordyceps militaris powder, 6-15 parts of pine pollen, 5-15 parts of selenium-rich malt powder, 3-12 parts of naked algae powder, 20-70 parts of fruit and vegetable coarse cereal powder and 0-5 parts of stevioside.
2. The health food for inhibiting tumor and improving immunity according to claim 1, which is prepared from the following raw materials in parts by weight: 15-20 parts of cordyceps militaris powder, 10-13 parts of pine pollen, 9-13 parts of selenium-rich malt powder, 6-8 parts of euglena powder, 48-60 parts of fruit and vegetable coarse cereal powder and 0-2 parts of stevioside.
3. The health food for suppressing tumor and enhancing immunity according to claim 1 or 2, wherein the cordyceps militaris powder is prepared by the following method: the artificially cultured fresh cordyceps militaris sporocarp is prepared by drying, crushing and sieving processes, wherein the content of cordycepin is 1 wt%.
4. The health food for inhibiting tumor and improving immunity according to claim 1 or 2, wherein the pine pollen is prepared by the following method: manually collected pollen pini is sieved by a 120-mesh sieve, and the pollen pini is subjected to jet milling to break the wall, wherein the content of crude polysaccharide is 1.5 wt%.
5. The health food for suppressing tumor and enhancing immunity according to claim 1 or 2, wherein the selenium-rich malt flour is prepared from selenium-rich malt sprouts by cleaning, drying and pulverizing, and the selenium content is 30-50 mg/kg.
6. The health food for suppressing tumor and enhancing immunity according to claim 1 or 2, wherein the gymnocyanine powder is prepared by culturing, concentrating, drying and sieving seeds of gymnocyanine, and has a β -glucan content of 50 wt%.
7. The health food for inhibiting tumor and improving immunity according to claim 1 or 2, wherein the coarse cereal fruit and vegetable powder is one or more selected from orange powder, pumpkin powder, carrot powder and malt powder.
8. The method for preparing a health food for suppressing tumor and enhancing immunity according to any one of claims 1 to 7, comprising the steps of: weighing the raw materials, uniformly mixing, and filling to obtain the product.
9. The use of the health food for suppressing tumor and enhancing immunity according to any one of claims 1 to 7 as a diet therapy product for suppressing tumor and enhancing immunity, wherein the oral dose is 5 to 10 g/day.
10. The use of claim 9, wherein the tumor is human lung cancer cell a549, human breast cancer cell MCF-7, or human chronic myelogenous leukemia cell K562.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010769863.0A CN111743152A (en) | 2020-08-03 | 2020-08-03 | Health food for inhibiting tumor and improving immunity and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010769863.0A CN111743152A (en) | 2020-08-03 | 2020-08-03 | Health food for inhibiting tumor and improving immunity and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111743152A true CN111743152A (en) | 2020-10-09 |
Family
ID=72712857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010769863.0A Pending CN111743152A (en) | 2020-08-03 | 2020-08-03 | Health food for inhibiting tumor and improving immunity and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111743152A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101380102A (en) * | 2008-09-25 | 2009-03-11 | 杭州金日生物科技有限公司 | Selenium rich pine pollen health food and preparation method thereof |
| CN102246956A (en) * | 2011-05-25 | 2011-11-23 | 郭景龙 | Healthcare food containing pollen pini and preparation method thereof |
| CN104522665A (en) * | 2014-12-30 | 2015-04-22 | 中恩（天津）医药科技有限公司 | Enteral full-nutrition preparation suitable for tumor patients at palliative treatment period and preparation method thereof |
| KR20160112836A (en) * | 2015-03-20 | 2016-09-28 | 한국 한의학 연구원 | Composition for enhancing innate immunity and antivirus comprising Pini Pollen extract as effective component |
| CN106616947A (en) * | 2017-01-03 | 2017-05-10 | 南京糖防生物科技有限公司 | Cordyceps militaris composition |
| CN106668560A (en) * | 2017-03-15 | 2017-05-17 | 新时代健康产业（集团）有限公司 | Pollen pini cordyceps composition with functions of cancer-prevention and cancer-resistance and preparation method thereof |
| CN110419727A (en) * | 2019-09-03 | 2019-11-08 | 生生源(大连)科技有限公司 | It effectively can prevent and improve the natural mineral matter replenishers of tumour |
-
2020
- 2020-08-03 CN CN202010769863.0A patent/CN111743152A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101380102A (en) * | 2008-09-25 | 2009-03-11 | 杭州金日生物科技有限公司 | Selenium rich pine pollen health food and preparation method thereof |
| CN102246956A (en) * | 2011-05-25 | 2011-11-23 | 郭景龙 | Healthcare food containing pollen pini and preparation method thereof |
| CN104522665A (en) * | 2014-12-30 | 2015-04-22 | 中恩（天津）医药科技有限公司 | Enteral full-nutrition preparation suitable for tumor patients at palliative treatment period and preparation method thereof |
| KR20160112836A (en) * | 2015-03-20 | 2016-09-28 | 한국 한의학 연구원 | Composition for enhancing innate immunity and antivirus comprising Pini Pollen extract as effective component |
| CN106616947A (en) * | 2017-01-03 | 2017-05-10 | 南京糖防生物科技有限公司 | Cordyceps militaris composition |
| CN106668560A (en) * | 2017-03-15 | 2017-05-17 | 新时代健康产业（集团）有限公司 | Pollen pini cordyceps composition with functions of cancer-prevention and cancer-resistance and preparation method thereof |
| CN110419727A (en) * | 2019-09-03 | 2019-11-08 | 生生源(大连)科技有限公司 | It effectively can prevent and improve the natural mineral matter replenishers of tumour |
Non-Patent Citations (2)
| Title |
|---|
| 张文: "《海洋药物导论》", 30 September 2012, 上海科学技术出版社 * |
| 黄淑芳等: "《走出亚健康》", 31 October 2002, 内蒙古人民出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111670997B (en) | Preparation method of immunity-enhancing compound protein peptide enzymatic hydrolysate, immunity-enhancing compound protein peptide beverage and preparation method thereof | |
| CN101023802A (en) | Series products of all-nutrient green health-care bean vermicellic and instant bean vermicellic and preparing method | |
| CN103653144B (en) | Healthcare drink capable of improving immunity and preparation method thereof | |
| CN108294231A (en) | It is suitble to the nutritional staple food of patients with diabetes mellitus | |
| CN104146300A (en) | Compound Noni juice and preparation method thereof | |
| CN106509525A (en) | Glossy ganoderma composite fruit juice health-care beverage and preparation method thereof | |
| CN108208504B (en) | Cowberry fruit health-care beverage | |
| CN103300446A (en) | Functional blueberry beverage composition | |
| KR20200087900A (en) | health food composition using pomegranate fermented by lactic acid bacteria and manufacturing method thereof | |
| KR101759494B1 (en) | Preparation method for sodium-reduced soy sauce with anti-hypertensive activity | |
| KR20160059137A (en) | MANUFACTURE OF FERMENTED Alliumhookeri FROM LACTIC ACID BACTERIA AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST | |
| CN107259003A (en) | A kind of lycium ruthenicum Moringa ginseng health protection tea and its application | |
| CN111743152A (en) | Health food for inhibiting tumor and improving immunity and preparation method thereof | |
| CN103349079B (en) | A kind of production method of functional milk beverage with cepe | |
| CN106497722B (en) | Walnut flower pomegranate fruit wine and preparation method thereof | |
| CN103103090A (en) | Silkworm chrysalis cordyceps wine and preparation method thereof | |
| CN103103091A (en) | Silkworm chrysalis cordyceps bamboo wine and preparation method thereof | |
| CN107897719B (en) | Natto composite preparation and preparation method thereof | |
| CN101530210B (en) | Health preserving wine | |
| CN105087284A (en) | Compound raspberry health wine and preparation method thereof | |
| CN109223819A (en) | Hundred tail gracilis polysaccharides are for the purposes in immunological regulation and anti-tumor drug | |
| CN113491331B (en) | Algal polysaccharide composition and compound fruit juice mixed fermentation powder as well as preparation method and application thereof | |
| KR100512897B1 (en) | Healthy beverages using condensed brewing vinegar and manufacturing method thereof | |
| KR102636322B1 (en) | Method for manufacturing dried yellow corvina with gochujang and dried yellow corvina with gochujang produced thereby | |
| CN108452047B (en) | Composition for delaying senility, inhibiting growth of tumor cells and improving cardiovascular and cerebrovascular microcirculation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201009 |
|
| RJ01 | Rejection of invention patent application after publication |