CN111729030A

CN111729030A - Compound bone collagen and preparation method thereof

Info

Publication number: CN111729030A
Application number: CN202010799766.6A
Authority: CN
Inventors: 阮树月
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-08-11
Filing date: 2020-08-11
Publication date: 2020-10-02

Abstract

The invention discloses a compound bone collagen which is prepared from the following raw materials in parts by weight: 400 portions of fish skin, 50 to 60 portions of sesame, 1 to 3 portions of herba cistanche, 1 to 3 portions of day lily, 1 to 3 portions of ginseng, 2 to 8 portions of cartilage mixture, 0.3 to 0.7 portion of fructose calcium borate and 1.8 to 2.7 portions of chondroitin sulfate. The invention selects high-quality calcium source, adopts the mixture of the pure natural fish skin and the cartilage as the calcium source, is easy to digest and absorb, is particularly suitable for long-term administration by middle-aged and elderly people, is safe and has no toxic or side effect; the scientific compatibility of the ossein protein can inhibit the loss of calcium from bones and improve the absorption of the calcium; but also can increase the bone density, slow down the aging of bone marrow, increase the toughness, strength and elasticity of bones, inhibit the reduction of bone mass, improve the metabolism of bones, regulate bone cells and increase the toughness of bones.

Description

Compound bone collagen and preparation method thereof

Technical Field

The invention relates to the technical field of health products, in particular to compound bone collagen and a preparation method thereof.

Background

Osteoporosis is a disease in which the systemic bone mass is reduced, the microstructure of bone tissue is changed, the brittleness of bone is increased, the strength of bone is reduced, and fracture can occur under the condition of no wound or mild wound. With the age, as the body's ability to absorb calcium decreases, the rate of bone resorption exceeds the rate of bone formation, resulting in bone loss and, in turn, osteoporosis. Osteoporosis not only affects normal work and life of people, but also causes a plurality of diseases and seriously affects the life quality of people. Meanwhile, postmenopausal osteoporosis caused by estrogen deficiency is a common disease of postmenopausal women, mainly occurs in the problems of bone mass reduction and bone tissue structure change, increased bone fragility, easy fracture, pain caused by fracture, bone deformation, complication, even death and the like, seriously affects the health and the life quality of old people, and even shortens the service life.

At present, the method for preventing and treating osteoporosis mainly supplements a proper amount of calcium besides strengthening exercise and strengthening dietary nutrition for food supplement. The existing calcium supplement preparations comprise active calcium ions, calcium gluconate, calcium lactate, calcium tablets and the like, most of the calcium supplement preparations or health-care foods only pay attention to the content of calcium in the products, and do not pay attention to the absorption and utilization degree of the calcium by human bodies, so that the supply amount of the calcium supplement is large, the absorption and utilization amount is small, and the ideal curative effect is difficult to achieve; meanwhile, middle-aged and elderly people suffering from osteoporosis are often accompanied by pain, inflammation, stiffness, swelling, deformity and dysfunction of joints of hands, wrists, ankles, knees, hips, shoulders, spines and the like caused by joint cartilage degradation, various calcium supplement products sold in the market at present mainly increase bone density, increase bone hardness and have limited effect on symptoms such as osteoarthritis, bone pain and the like.

Disclosure of Invention

In view of the above technical deficiencies, the present invention provides a compound collagen and a preparation method thereof, so as to solve the problems in the background art.

In order to solve the technical problems, the invention adopts the following technical scheme:

the invention provides compound bone collagen which is prepared from the following raw materials in parts by weight:

400 portions of fish skin, 50 to 60 portions of sesame, 1 to 3 portions of herba cistanche, 1 to 3 portions of day lily, 1 to 3 portions of ginseng, 2 to 8 portions of cartilage mixture, 0.3 to 0.7 portion of fructose calcium borate and 1.8 to 2.7 portions of chondroitin sulfate.

The compound bone collagen is prepared from the following raw materials in parts by weight:

400 parts of fish skin, 50 parts of sesame, 1 part of herba cistanche, 1 part of day lily, 1 part of ginseng, 2 parts of cartilage mixture, 0.3 part of fructose calcium borate and 1.8 parts of chondroitin sulfate.

500 parts of fish skin, 60 parts of sesame, 3 parts of herba cistanche, 3 parts of day lily, 3 parts of ginseng, 8 parts of cartilage mixture, 0.7 part of fructose calcium borate and 2.7 parts of chondroitin sulfate.

450 parts of fish skin, 55 parts of sesame, 2 parts of herba cistanche, 2 parts of day lily, 2 parts of ginseng, 5 parts of cartilage mixture, 0.5 part of fructose calcium borate and 2.3 parts of chondroitin sulfate.

460 parts of fish skin, 57 parts of sesame, 1.7 parts of herba cistanche, 2.4 parts of day lily, 1.2 parts of ginseng, 6 parts of cartilage mixture, 0.6 part of calcium fructoborate and 2.5 parts of chondroitin sulfate.

A preparation method of compound bone collagen comprises the following steps:

(1) pretreatment of fish skin:

a. putting the fish skin into clear water, rinsing, changing the clear water, adding hydrochloric acid to adjust the pH value to 2-5, and removing foreign proteins on the surface of the fish skin; soaking the fish skin with the foreign proteins removed in clear water, and washing until the pH value is neutral;

b. cutting fish skin into small pieces, stirring with sodium bicarbonate solution with a material-liquid ratio of 1: 20(w/v) and a mass concentration of 10% for 24h, changing the solution every 8h, washing at 30 ℃, stirring with n-butyl alcohol solution with a material-liquid ratio of 1: 20(w/v) and a volume concentration of 10% for 48h, changing the solution every 12h, washing at 30 ℃, stirring with AXAUT-50 non-ionic degreasing agent with a material-liquid ratio of 1: 20(w/v) and a volume concentration of 5% for 36h, changing the solution every 12h, and washing at 30 ℃; then placing the fish skin into hydrogen peroxide solution with the material-liquid ratio of 1: 20(w/v), the pH value of 11 and the volume concentration of 1 percent, stirring for 12 hours, changing the solution every 8 hours, and washing with distilled water; adding into Tris-HCl buffer solution (NaCl content 10%) with a material-liquid ratio of 1: 20(w/v), pH of 7.5 and 0.05mol/L, stirring for 12h, changing solution every 8h, and washing with distilled water to obtain processed gelatin extraction raw material;

(2) extraction of fish skin gelatin: placing the gelatin extraction raw material pretreated in the step (1) into an acid solution with a material-liquid ratio of 1: 20-50 (w/v) and a concentration of 0.1-0.5 mol/L, and stirring and extracting for 48-72 h in an ice-water bath to obtain an undenatured fish skin collagen solution;

(3) parching semen Sesami, and grinding into semen Sesami powder;

(4) adding 30-50 times of drinking water into the mixture of herba Boschniakiae Rossicae, flos Hemerocallis, Ginseng radix, and cartilage, decocting for 5-6 hr, cooling, filtering, and concentrating the filtrate to 20-30 wt% to obtain Chinese medicinal liquid;

(5) filtering the gelatin solution obtained in the step (2) to remove impurities, concentrating by using a hollow fiber membrane with the molecular weight cutoff of 50-100ku until the mass fraction is 20-30%, adding the Chinese medicinal liquid prepared in the step (4), fructose calcium borate, manganese and chondroitin sulfate, and keeping the temperature at 35-45 ℃ for 30-60 min;

(6) and (3) drying: adding sesame powder into the gelatin solution obtained in the step (5), uniformly stirring, cooling and solidifying at 4-10 ℃, cutting into slices or strips, drying at low temperature, and crushing by a crusher to prepare the fish skin gelatin powder.

The invention has the beneficial effects that:

firstly, compared with other existing products, the fish skin calcium source has a higher quality calcium source, and the mixture of the pure natural fish skin and the cartilage is used as the calcium source, so that the fish skin calcium source is easy to digest and absorb, is particularly suitable for long-term administration by middle-aged and elderly people, and is safe and free of toxic and side effects; the scientific compatibility of the ossein protein can inhibit the loss of calcium from bones and improve the absorption of the calcium; but also can increase the bone density, slow down the aging of bone marrow, increase the toughness, strength and elasticity of bones, inhibit the reduction of bone mass, improve the metabolism of bones, regulate bone cells and increase the toughness of bones.

Secondly, the invention applies the theories of modern medicine and nutriology, combines the theories of the classical traditional Chinese medicine, combines the traditional Chinese medicine and the western medicine, exerts the unique advantages of the traditional Chinese medicine in the aspects of preventing and treating the chronic diseases of the middle-aged and the elderly and recovering and preserving health, and comprehensively supplements calcium and nourishes bones according to the characteristics of human pathophysiology.

Thirdly, boron, manganese and chondroitin sulfate are added in the formula, and the boron can reduce calcium loss, promote VD and magnesium functions, help bone health and promote the absorption of nutrition from healthy bones of joints.

Clinical trial preliminary studies show that osteoporosis patients can treat osteoporosis, long-term lumbago, leg pain and scapulohumeral periarthritis after taking the product, and clinical symptoms such as bone pain, cramp and the like are remarkably relieved.

Drawings

FIG. 1 is a bar graph of calcium absorption rate of test samples of the present invention.

Detailed Description

Example 1

Example 2

Example 3

Example 4

The method for preparing the compound collagen of examples 1 to 4 includes the steps of:

(1) pretreatment of fish skin:

(3) parching semen Sesami, and grinding into semen Sesami powder;

Test examples

< evaluation test of calcium absorption Effect >

The amount of calcium ingested by the rat and the amount of calcium excreted in the feces were measured under steady state, and the difference between the two was the apparent calcium absorbed. Apparent absorption rate (%) of calcium (intake of calcium-fecal calcium)/intake of calcium × 100%. Since the absorption rate of calcium is greatly affected by age, sex, feed ingredients and calcium intake level of rats, the absorption rate of calcium in a test sample should be evaluated by comparing the absorption rates of calcium carbonate and the test sample under the condition that other influencing factors are as the same as possible.

Selecting weaning rats at four weeks of birth, and raising the rats in cages for 4 weeks after the adaptation period of 1 week. Each group had at least 8 animals of the same sex. Deionized water was drunk to avoid calcium pick up from drinking water. Body length and body weight were measured once a week. The blended low-calcium feed is used as a basic feed. The test set was a control group and each example group. Three example groups were set up simultaneously with a blank control group and a calcium carbonate control group at the same calcium level as the corresponding dose of the test substance.

Groups of rats were treated as follows: blank control group: free eating and drinking; positive control group: the dosage of the calcium carbonate is 3.6 mg/kg.d, and the administration is carried out by gastric lavage; high dose group: the compound collagen prepared in example 4 of the present invention was prepared at a dose of 2.7 mg/kg. d and administered by gavage; the medium dose group: the compound collagen prepared in example 4 of the present invention was prepared at a dose of 1.8 mg/kg. d and administered by gavage; low dose group: the compound collagen prepared in example 4 of the present invention was prepared at a dose of 0.9 mg/kg. d, and administered by gavage.

Calcium metabolism experiments were performed 3 days after 3 weeks. And (4) recording the food intake of 3 days, collecting feces for 72 hours, and measuring the calcium content in the feed and the feces. The calcium in the feed and the feces is measured by atomic absorption spectrophotometry. Calcium intake (mg/d) is the calcium content in the feed (mg/g) x feed consumption (g/d); fecal calcium (mg/d) refers to calcium content in feces (mg/g) x fecal output (g/d). The results are shown in FIG. 1.

As can be seen in fig. 1, the absorption of calcium in the test sample was significantly higher for different doses of example 4 than for the same level of calcium carbonate. Therefore, the collagen polypeptide compound medicine can promote the absorption of calcium.

< clinical test >

1.1 study object

100 patients with osteoporosis were selected as the study subjects, and all patients were examined for medical history, blood sugar, biochemistry and the like, and were excluded from taking hormones, vitamin D, lumbar disc herniation, ankylosing spondylitis, tumors, pregnant women and the like within 1 year. According to random grouping, the control group comprises 50 cases, 23 cases of men, 27 cases of women, the age of 58-77 years, and the average age (66.2 +/-4.2) years; the course of disease is 2-34 years, and the average course of disease (14.8 +/-2.5). Observation group 50, 26 men, 24 women, age 61-79 years, mean age (64.8 ± 3.5) years; the course of disease is 2-33 years, and the average course of disease (15.2 +/-3.4) years. Wherein the female menopause accounts for 90 percent of female cases, compared with general data such as age, sex, course of disease and the like of two groups of patients, the difference has no statistical significance and has contrast.

1.2 methods of treatment

Control group: calcium carbonate D3 tablets were administered to control patients; specification: 500 mg/tablet, 1 tablet/time, 2 times/day, free diet;

observation group: patients in the observation group were administered the compound bone collagen of example 4 of the present invention; specification: 500 mg/time, 2 times/day, free diet.

1.3 Observation index

After 6 months, the treatment effect and the adverse reaction condition in the period of the treatment are compared between two groups of patients. Before and after treatment, the lumbar vertebra and the femoral neck of the patient are measured by adopting an X-ray bone densitometer; detecting the content of bone markers including calcitonin (BGP), bone specific alkaline phosphatase (BALP) and carboxyl terminal state of type 1 Collagen (CTX) in patients before and after treatment.

1.4 evaluation index

After 6 weeks of continuous treatment, the clinical efficacy and incidence of complications were compared between the two groups of patients. The method has the following advantages: the osteoporosis of the patient can be improved, and the bone density is increased; and (4) invalidation: the osteoporosis of the patient is serious, and the bone density is unchanged.

The changes of bone density and bone marker content before and after treatment are shown in table 1, and the effective rate of clinical curative effect is shown in table 2.

TABLE 1 Change in bone Density and bone marker content before and after treatment in two groups of patients

TABLE 2 comparison of clinical efficacy of two groups of subjects

Group of	Adverse reactions (example)	Working examples	Examples of invalidity	High efficiency
					Control group	6	39	5	78％
Observation group	1	47	2	94％

As can be seen from tables 1-2, the total effective rate of the patients in the observation group is 94%, the total effective rate of the patients in the control group is 78%, the total effective rate of the observation group is significantly higher than that of the control group, and the bone density of the lumbar vertebrae and the femoral neck of the observation group after treatment is significantly higher than that of the control group. The collagen of the invention has better effect on treating osteoporosis than calcium carbonate D3, promotes the absorption of calcium, and can effectively supplement calcium, thereby rapidly improving bone density.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. The compound bone collagen is characterized by being prepared from the following raw materials in parts by weight:

2. The compound bone collagen according to claim 1, which is prepared from the following raw materials in parts by weight:

3. The compound bone collagen according to claim 1, which is prepared from the following raw materials in parts by weight:

4. The compound bone collagen according to claim 1, which is prepared from the following raw materials in parts by weight:

5. The compound bone collagen according to claim 1, which is prepared from the following raw materials in parts by weight:

6. The preparation method of the compound bone collagen is characterized by comprising the following steps:

(1) pretreatment of fish skin:

(3) parching semen Sesami, and grinding into semen Sesami powder;