CN111718922A - Special enzyme for rubber and method for removing protein from natural rubber - Google Patents
Special enzyme for rubber and method for removing protein from natural rubber Download PDFInfo
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- CN111718922A CN111718922A CN202010786647.7A CN202010786647A CN111718922A CN 111718922 A CN111718922 A CN 111718922A CN 202010786647 A CN202010786647 A CN 202010786647A CN 111718922 A CN111718922 A CN 111718922A
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- rubber
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- enzyme preparation
- compound enzyme
- enzyme
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08C—TREATMENT OR CHEMICAL MODIFICATION OF RUBBERS
- C08C2/00—Treatment of rubber solutions
- C08C2/02—Purification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22002—Papain (3.4.22.2)
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- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
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- Biotechnology (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a rubber special enzyme and a method for removing protein from natural rubber, wherein the rubber special enzyme comprises a rubber special compound enzyme preparation 1 and a rubber special compound enzyme preparation 2, and the rubber special compound enzyme preparation 1 consists of 50% of alkaline protease and 50% of papain; the special compound enzyme preparation 2 for rubber consists of 50 percent of acid protease and 50 percent of neutral protease. The activity of the complex enzyme preparation 1 special for rubber is 200000 u/g. The activity of the complex enzyme preparation 2 special for rubber is 50000 u/g. The rubber special enzyme can degrade and remove protein components in natural rubber clear liquid, and can well solve the problem that small peptides caused by the protease hydrolysis process influence the proliferation of sewage bacteria. The protein removal method of the natural rubber overcomes the defects in the prior art, and the rubber special enzyme used in the method can reduce the sewage treatment difficulty and improve the extraction quality of the natural rubber.
Description
Technical Field
The invention belongs to the technical field of rubber special enzyme, and particularly relates to a rubber special enzyme and a natural rubber protein removal method.
Background
Rubber is generally divided into synthetic rubber and natural rubber, the synthetic rubber has poor toughness but good conductivity, and the natural rubber has poor conductivity, and part of the reason is that the natural rubber contains vegetable protein but the synthetic rubber does not contain vegetable protein, and the protein in the natural rubber needs to be removed in order to improve the conductivity of the natural rubber. Use alkaline protease desorption protein in the natural rubber at present in production, use the alkaline protease after to exert an influence to sewage fungus, be unfavorable for reforming transform the sewage treatment problem, the problem of its existence has: 1. the bacterial content of the alkaline protease is too high, so that competitive inhibition is caused; 2. the alkaline protease is a bactericidal enzyme and affects the growth of the sewage bacteria; 3. the alkaline protease generates bacteriostatic small peptides when cutting protein molecules, and generates bacteriostatic suppression on sewage bacteria. Based on the above defects in the prior art, there is a need to develop a rubber-specific enzyme and a method for removing proteins from natural rubber.
Disclosure of Invention
The invention provides a rubber special enzyme and a natural rubber protein removal method in order to overcome the defects of the prior art.
The invention is realized by the following technical scheme: the invention discloses a rubber special enzyme, which comprises a rubber special compound enzyme preparation 1 and a rubber special compound enzyme preparation 2, wherein the rubber special compound enzyme preparation 1 consists of alkaline protease and papain; the special compound enzyme preparation 2 for rubber consists of acid protease and neutral protease.
Preferably, the complex enzyme preparation 1 specially used for rubber consists of 50 percent of alkaline protease and 50 percent of papain in percentage by mass. The special compound enzyme preparation 2 for rubber consists of 50 percent of acid protease and 50 percent of neutral protease by mass percent. The activity of the complex enzyme preparation 1 special for rubber is 200000 u/g. The activity of the complex enzyme preparation 2 special for rubber is 50000 u/g.
The invention also discloses a method for removing the protein of the natural rubber by using the special enzyme for the rubber, which comprises the following steps: (1) separating the natural rubber fresh glue solution into a glue solution after passing through a centrifugal machine, and keeping the temperature of the glue solution at 38-42 ℃; (2) respectively dissolving the special compound enzyme preparation 1 for rubber and the special compound enzyme preparation 2 for rubber by using water; (3) firstly, adding the special complex enzyme preparation 1 for rubber into the rubber clear liquid according to the mass percent of 0.1% in a stirring tank, and stirring for 60-90 min; (4) adding the special rubber compound enzyme preparation 1 for 60-90 min, and then adding the special rubber compound enzyme preparation 2, wherein the stirring is continuously carried out in the adding process without staying; (5) and after the compound enzyme preparation 2 special for rubber is added, stirring for 10min, pumping the rubber clear liquid into an acid regulating reaction tank, and immediately regulating the acid to a standard value.
Preferably, the temperature of the skim latex in step (1) is maintained at 40 ℃. Stirring for 80min in the step (3). And (4) adding the special rubber compound enzyme preparation 1 for 70min, and then adding the special rubber compound enzyme preparation 2 according to the mass percent of 0.05%.
The invention has the beneficial effects that: the invention discloses a rubber special enzyme and a natural rubber protein removal method. The main functions of the special enzyme for rubber of the invention are as follows: the special enzyme for rubber can well solve the problem that small peptides caused by the hydrolysis process of protease influence the proliferation of the sewage bacteria. The protein removal method of the natural rubber overcomes the defects in the prior art, and the rubber special enzyme used in the protein removal method can reduce the sewage treatment difficulty and improve the extraction quality of the natural rubber.
Detailed Description
The present invention will be described in detail with reference to specific embodiments.
The invention discloses a rubber special enzyme, which comprises a rubber special compound enzyme preparation 1 and a rubber special compound enzyme preparation 2, wherein the rubber special compound enzyme preparation 1 consists of alkaline protease and papain; the special compound enzyme preparation 2 for rubber consists of acid protease and neutral protease. The special compound enzyme preparation 1 for rubber consists of 50 percent of alkaline protease and 50 percent of papain in percentage by mass. The special compound enzyme preparation 2 for rubber consists of 50 percent of acid protease and 50 percent of neutral protease by mass percent. The activity of the complex enzyme preparation 1 special for rubber is 200000 u/g. The activity of the complex enzyme preparation 2 special for rubber is 50000 u/g.
The invention also discloses a method for removing the protein of the natural rubber by using the special enzyme for the rubber, which comprises the following steps: (1) separating the natural rubber fresh glue solution from the natural rubber fresh glue solution through a centrifugal machine, and keeping the temperature of the glue solution at 40 ℃; (2) respectively dissolving the special compound enzyme preparation 1 for rubber and the special compound enzyme preparation 2 for rubber by using water; (3) firstly, adding the special complex enzyme preparation 1 for rubber into the rubber clear liquid according to the mass percent of 0.1% in a stirring tank, and stirring for 60-90 min; (4) adding the special rubber compound enzyme preparation 1 for 60-90 min, then adding the special rubber compound enzyme preparation 2 according to the mass percent of 0.05%, and continuously stirring without staying in the adding process; (5) and after the compound enzyme preparation 2 special for rubber is added, stirring for 10min, pumping the rubber clear liquid into an acid regulating reaction tank, and immediately regulating the acid to a standard value.
The packaging specification of the rubber special enzyme is 20 kg/bag or 25 kg/bag. It should be stored in a sealed and dark place, and placed in a cool, dry and ventilated place. The enzyme product in the invention is easy to absorb moisture, and can be used as soon as possible after being unsealed, and if the enzyme product can not be used in the same day, the bag opening is tied and placed in a dry place. The storage warehouse needs to be kept clean, cool, dry and ventilated, and is protected from being heated and damp. Can not be mixed with toxic and harmful substances and other pollutants for storage, and can be optimally used within 12 months of shelf life.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. A rubber special enzyme is characterized in that: the rubber special enzyme comprises a rubber special compound enzyme preparation 1 and a rubber special compound enzyme preparation 2, wherein the rubber special compound enzyme preparation 1 consists of alkaline protease and papain; the special compound enzyme preparation 2 for rubber consists of acid protease and neutral protease.
2. The enzyme dedicated to rubber according to claim 1, wherein: the compound enzyme preparation 1 special for rubber is composed of 50% of alkaline protease and 50% of papain by mass percent.
3. The enzyme dedicated to rubber according to claim 1, wherein: the special compound enzyme preparation 2 for rubber is composed of 50% of acid protease and 50% of neutral protease in percentage by mass.
4. The enzyme dedicated to rubber according to claim 1, wherein: the activity of the special compound enzyme preparation 1 for rubber is 200000 u/g.
5. The enzyme dedicated to rubber according to claim 1, wherein: the activity of the special compound enzyme preparation 2 for rubber is 50000 u/g.
6. The method for protein removal from natural rubber using rubber-specific enzyme according to claim 1, 2 or 3, wherein: the method for removing the protein from the natural rubber specifically comprises the following steps:
(1) separating the natural rubber fresh glue solution into a glue solution after passing through a centrifugal machine, and keeping the temperature of the glue solution at 38-42 ℃;
(2) respectively dissolving the special compound enzyme preparation 1 for rubber and the special compound enzyme preparation 2 for rubber by using water;
(3) firstly, adding the special complex enzyme preparation 1 for rubber into the rubber clear liquid according to the mass percent of 0.1% in a stirring tank, and stirring for 60-90 min;
(4) adding the special rubber compound enzyme preparation 1 for 60-90 min, and then adding the special rubber compound enzyme preparation 2, wherein the stirring is continuously carried out in the adding process without staying;
(5) and after the compound enzyme preparation 2 special for rubber is added, stirring for 10min, pumping the rubber clear liquid into an acid regulating reaction tank, and immediately regulating the acid to a standard value.
7. The method of claim 6, wherein the removal of proteins from natural rubber is performed by using a rubber-specific enzyme: the temperature of the skim latex in the step (1) is kept at 40 ℃.
8. The method of claim 6, wherein the removal of proteins from natural rubber is performed by using a rubber-specific enzyme: stirring for 80min in the step (3).
9. The method of claim 6, wherein the removal of proteins from natural rubber is performed by using a rubber-specific enzyme: and (4) adding the special rubber compound enzyme preparation 1 for 70min, and then adding the special rubber compound enzyme preparation 2 according to the mass percent of 0.05%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010786647.7A CN111718922A (en) | 2020-08-07 | 2020-08-07 | Special enzyme for rubber and method for removing protein from natural rubber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010786647.7A CN111718922A (en) | 2020-08-07 | 2020-08-07 | Special enzyme for rubber and method for removing protein from natural rubber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111718922A true CN111718922A (en) | 2020-09-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010786647.7A Pending CN111718922A (en) | 2020-08-07 | 2020-08-07 | Special enzyme for rubber and method for removing protein from natural rubber |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017017178A2 (en) * | 2015-07-30 | 2017-02-02 | Compagnie Generale Des Etablissements Michelin | Process for manufacturing a rubber composition based on natural rubber |
| WO2017046204A1 (en) * | 2015-09-17 | 2017-03-23 | Compagnie Generale Des Etablissements Michelin | Method for producing a natural rubber |
| CN110922509A (en) * | 2019-12-04 | 2020-03-27 | 河南崤函生物科技有限公司 | Deproteinized rubber and preparation method thereof |
-
2020
- 2020-08-07 CN CN202010786647.7A patent/CN111718922A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017017178A2 (en) * | 2015-07-30 | 2017-02-02 | Compagnie Generale Des Etablissements Michelin | Process for manufacturing a rubber composition based on natural rubber |
| WO2017046204A1 (en) * | 2015-09-17 | 2017-03-23 | Compagnie Generale Des Etablissements Michelin | Method for producing a natural rubber |
| CN110922509A (en) * | 2019-12-04 | 2020-03-27 | 河南崤函生物科技有限公司 | Deproteinized rubber and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| SIRAWAN PRASERTKITTIKUL等: "Deproteinization of Natural Rubber Using Protease Immobilized onEpichlorohydrin Cross-linked Chitosan Beads" * |
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