CN109892661A - A kind of pearl shell peptide and its production method - Google Patents
A kind of pearl shell peptide and its production method Download PDFInfo
- Publication number
- CN109892661A CN109892661A CN201910236795.9A CN201910236795A CN109892661A CN 109892661 A CN109892661 A CN 109892661A CN 201910236795 A CN201910236795 A CN 201910236795A CN 109892661 A CN109892661 A CN 109892661A
- Authority
- CN
- China
- Prior art keywords
- pearl shell
- shell peptide
- pearl
- production method
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to pearl shell peptide field, a kind of pearl shell peptide and its production method are disclosed.The production method of the pearl shell peptide includes by pearl shell through raw material pre-treatment, high efficiency compounded enzyme preparate digests under the conditions of not adjusting pH value and not controlling temperature, and inorganic nanofiltration ceramic membrane filter removal not readily dissolves the ingredient with the easy moisture absorption, the high temperature high pressure process of peptide solution, concentration, dry powder processed.Do not need to adjust enzymatic hydrolysis pH value using method provided by the invention, do not need that controlled enzymatic hydrolysis temperature, enzymolysis time are short, enzyme additive amount is few, enzymolysis product molecular weight is low and concentrate, pearl shell peptide powder water solubility obtained is splendid and storage in be not easy moisture absorption agglomeration.
Description
Technical field
The invention belongs to pearl shell peptide field, in particular to a kind of production method of pearl shell peptide.
Background technique
Pearl shell is the main shellfish of China's pearl culture.Pearl shell adopts the shellfish meat left after pearl rich in protein, at present
Other than a small amount of fresh eating, be mainly used for being processed into the low value-added product such as feed, can, flavouring, make pearl oyster meat this
Valuable natural resources cannot be utilized adequately.
CN101982114A discloses a kind of production method of anti-fatigue oral liquids by using Pinctada martensii dunker meats, is to use high-speed organization
Pearl oyster meat is pounded pulpous state by bruisher, and No. 6 light petrol stirring degreasings are added except fishy smell, centrifugation remove No. 6 light petrols, collect precious
Pearl shellfish meat slurry adds water and stirs uniformly heating, first adds trypsin digestion by pearl oyster meat slurry quality, inactivates pancreas after the completion of enzymatic hydrolysis
Protease；Add papain enzymolysis by pearl oyster meat slurry quality again, inactivates papain after the completion of enzymatic hydrolysis；Then pearl is pressed
Shellfish meat slurry quality adds alkali protease to digest, and enzymatic hydrolysis inactivates alkali protease after completing, and is cooled to room temperature；It is collected by centrifugation water-soluble
Liquid vacuum concentration, is diluted to properly with sterile water, adds ethylmaltol, citric acid, honey element, is dissolved, and is uniformly mixed and production is made
Product.This method is simple for process, and the production cycle is shorter, and pearl oyster meat protein passes through three enzyme composite hydrolysis, the amino of generation
Acid and small peptide quantity obviously increase, and are conducive to the raising of product quality.
CN102220402A discloses a kind of method that antibacterial peptide is extracted from pearl oyster meat, includes the following steps: to learn from else's experience
Pearl oyster meat is pre-processed, is added after ice water carries out cooled homogenate and obtains homogenate；It is added after flavor protease mixes and adjusts in homogenate
The working frequency for saving ultrasonic wave is 35kHz, ultrasonic intensity is 280~360W, temperature is 50~65 DEG C and carries out ultrasonic treatment 90
~110min；Homogenate after ultrasonic treatment continues to digest 1~2h in 50~65 DEG C of waters bath with thermostatic control, then carries out enzyme deactivation and cold
But enzymolysis liquid is handled to obtain；Enzymolysis liquid is subjected to centrifugal treating, taking supernatant to adjust the operating pressure of UF membrane is 2~3bar, temperature
Degree is 3~10 DEG C, carries out hyperfiltration treatment using membrane separation technique, collects the separating liquid that molecular cut off is 5000~10000Da,
Pearl oyster meat antibacterial peptide product is obtained after freeze-drying.This method is extracted precious with ultrasonic technology auxiliary flavor protease hydrolysis
Antibacterial peptide in pearl shellfish meat, it is easy to operate, percent hydrolysis and bacteriostatic activity can be improved, shorten hydrolysis time, production cost is low, produces
Product safety can will open the pearl oyster meat resource higher value application discarded after pearl, improve the economic benefit of manufacturing enterprise.
However, problems and disadvantages existing for above-mentioned two pieces application for a patent for invention are as follows: the enzymolysis efficiency of the enzyme of use is low, enzymatic hydrolysis
Time is long, and enzyme additive amount is more, needs to adjust optimum pH and optimum temperature is digested, complicated for operation, adjusts peak enzymolysis-ability pH
Value can bring a large amount of inorganic ions into, and control peak enzymolysis-ability temperature energy consumption is larger；Use the film core of film filtering for organic material, intolerant to
Soda acid, non-refractory high pressure, flux is small, service life is short, and cleaning is inconvenient；The water solubility of prepared pearl shell peptide powder
It is poor, moisture absorption agglomeration is easy in storage.
Summary of the invention
The purpose of the present invention is to provide one kind not to need to adjust enzymatic hydrolysis pH value, does not need controlled enzymatic hydrolysis temperature, enzymatic hydrolysis production
Object molecular weight is low and concentrates, is made that pearl shell peptide powder water solubility is splendid and be not easy in storage the pearl of moisture absorption agglomeration
The production method of shellfish peptide and the pearl shell peptide produced by this method.
Specifically, the present invention provides a kind of production methods of pearl shell peptide, wherein method includes the following steps:
(1) raw material pre-treatment: by pearl shell decladding, taking pearl oyster meat, and the water of 1~4 times of raw material weight is added after cleaning up,
Tissue mashing heats 10~30min at homogenate at 80~100 DEG C later, then is cooled to 50~55 DEG C to enzymolysis liquid；
(2) it digests: toward described to which the high efficiency compound enzyme that dosage is raw material weight 0.05~0.15% is added in enzymolysis liquid
Preparation digests 0.5~2h in the case where not adjusting pH value, the natural conditions for not controlling temperature after mixing, obtains thick pearl shell peptide
Solution；The high efficiency compounded enzyme preparate by 20~25wt% compound protease, 20~25wt% alkali protease, 20~
25wt% neutral proteinase, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and 2~3wt% enzyme activition
Agent composition, the zymoexciter contain reducing agent and bivalent metal ion；In the zymoexciter reducing agent and divalent metal from
The molar ratio of son is 1:(0.5~2)；
(3) inorganic nanofiltration ceramic membrane filter: use molecular cut off for the inorganic of 800Da the thick pearl shell peptide solution
Nanofiltration ceramic membrane filter, gained permeate are high-purity pearl shell peptide solution；
(4) high temperature high pressure process: the high-purity pearl shell peptide solution is not less than in temperature not less than 110 DEG C, pressure
High temperature high pressure process is carried out under conditions of 0.1MPa, obtains treatment fluid；
(5) it is concentrated: the treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate；
(6) dry powder processed: the concentrate is spray-dried, pearl shell peptide powder is obtained.
Further, the molar ratio of reducing agent and bivalent metal ion is 1:(0.9~1.1 in the zymoexciter).
Further, the reducing agent is dithiothreitol (DTT) and/or beta -mercaptoethanol.
Further, the bivalent metal ion is Mg2+、Zn2+And Ca2+Mixture.
Further, Mg2+、Zn2+And Ca2+Molar ratio be (0.9~1.1): (0.9~1.1): 1.
Further, the filter core of the inorganic nanofiltration ceramic membrane is using in siliceous, aluminum, magnesia, sa, simatic
Any one material is made.
Further, the condition of the high temperature high pressure process include temperature be 110~130 DEG C, pressure be 0.1~
0.3MPa, time are 5~20min.
In addition, the present invention also provides the pearl shell peptides produced by the above method.
Key means of the invention first is that treating enzymatic hydrolysis using heat treatment and specific high efficiency compounded enzyme preparate
Liquid is digested, wherein the heat treatment enables to pearl oyster meat albuminous degeneration, it is made to be easier to be digested, and improves enzymatic hydrolysis
Efficiency, the high efficiency compounded enzyme preparate by 20~25wt% compound protease, 20~25wt% alkali protease, 20~
25wt% neutral proteinase, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and 2~3wt% enzyme activition
Agent (mixture of reducing agent and bivalent metal ion) composition, this specific compounded enzyme preparate is containing there are many can be directed to pearl
Endo protease, exoproteinase and the breaking cell wall enzyme that shellfish meat albumen is digested efficiently specificity can digest pearl oyster meat
Albumen, and reducing agent contained in zymoexciter and bivalent metal ion can activate the enzyme activity of these types of protease, it will
The enzyme preparation that above several protease compound formation under the action of reducing agent and bivalent metal ion according to the above ratio has
Extremely strong pearl oyster meat proteolysis efficiency.The pearl oyster meat albumen after heated is carried out using the above highly efficiency compositional enzyme preparation
Enzymatic hydrolysis can be realized thoroughly in the case where not adjusting pH value, not controlling the natural conditions of temperature under the premise of adding a small amount of protease
The purpose of pearl oyster meat albumen is digested, enzymolysis product molecular weight is small and concentrates, and enzymolysis product free aminoacid content is low, protein
Molecular weight is that 1000Da ratio below has reached 100%, and the pearl oyster meat protein peptides ratio between 200~1000Da reaches
95% or more.Furthermore, on the one hand, in enzymolysis process, do not need to adjust peak enzymolysis-ability pH value using soda acid, not bring into inorganic
Ion, it is subsequent not need that nanofiltration membrane is taken to remove inorganic ions again, simplify processing step；On the other hand, in enzymolysis process,
It does not need to adjust peak enzymolysis-ability temperature yet, compared with the prior art, not only easy to operate but also saving energy consumption.
To sum up, beneficial effects of the present invention are as follows:
(1) using the combination technique means of heat treatment and high efficiency compounded enzyme preparate enzymatic hydrolysis, it is precious to significantly improve enzymatic hydrolysis
The enzymolysis efficiency of pearl shellfish meat albumen, so that addition is a small amount of in the case where not adjusting optimum pH, not controlling the natural conditions of optimum temperature
Protease digests the short period, can thoroughly digest pearl oyster meat albumen, enzymolysis product molecular weight is small and concentrates, enzymolysis product
Free aminoacid content is low, and protein molecular weight is that 1000Da ratio below reaches 100%, between the ratio of 200~1000Da
Example has reached 95% or more.In addition, not needing to adjust peak enzymolysis-ability pH value using soda acid, not bringing nothing into enzymolysis process
Machine ion, it is subsequent not need that nanofiltration membrane is taken to remove inorganic ions again, simplify processing step；In enzymolysis process, do not need yet
Peak enzymolysis-ability temperature is adjusted, compared with the prior art, not only easy to operate but also saving energy consumption.
(2) technological means that inorganic nanofiltration ceramic membrane filter is used after enzymatic hydrolysis removes the substance that not readily dissolves and easily
The carbohydrate content of the moisture absorption improves the water solubility of finally obtained pearl shell peptide finished product, and is not easy to inhale during finished stock storage
Moisture knot block.Inorganic nanofiltration ceramic membrane has organic solvent-resistant, high temperature resistant, acid and alkali-resistance, mechanical strength compared with organic nanofiltration membrane
Advantage high, flux is big and the service life is long.The present invention need to only use a nanofiltration membrane treatment, bigger using ultrafiltration membrane retention than both needing
Molecular substance needs to compare using the prior art of nanofiltration membrane desalination and concentration again, significantly simplifies processing step, and film process
Effect it is different.
(3) gained peptide solution is used into high temperature high pressure process after ceramic membrane filter, can further increased so most
The water solubility of the pearl shell peptide finished product obtained eventually, finished product is finer and smoother, is highly soluble in water.
Specific embodiment
The present invention described in detail below.
In the present invention, term " pearl shell peptide " refers to the protein peptides produced using pearl oyster meat as raw material.
In the present invention, the effect of the raw material pre-treatment is sufficiently to mention the protein in pearl oyster meat and make pearl shell
Meat protein denaturation improves enzymolysis efficiency to be more advantageous to subsequent enzymatic hydrolysis.
In the present invention, the enzymatic hydrolysis carries out under the conditions of not adjusting pH value, not controlling temperature.It is precious after the enzymatic hydrolysis
Free aminoacid content is low, protein molecular weight is for can be degraded into after pearl shellfish meat albumen and heated denaturation to enzymolysis liquid
The enzymatic hydrolysis that the ratio of 1000Da reached for 100% (having reached 95% or more between the pearl shell peptide ratio of 200~1000Da) produces
Object.Wherein, protease contained in the high efficiency compounded enzyme preparate can pass through commercially available acquisition.For example, the compound egg
White enzyme can be the compound protease purchased from Novozymes Company.The alkali protease can be for purchased from Nanning Pang Bo biology
Engineering Co., Ltd is originated from the resulting alkali protease of the lichen bacillus ferments.The neutral proteinase can be for purchased from wide
Western Co., Ltd, Pangbo Bioengineering Co Ltd, Nanning is originated from the resulting alkali protease of fermentation of bacillus subtilis.The flavor albumen
Enzyme can be to be originated from the resulting alkali protease of aspergillus oryzae fermentation purchased from Nanning Pang Bo bioengineering Co., Ltd.It is described
Animal proteolytic enzyme can be the animal protein water from complex enzyme purchased from Nanning Pang Bo bioengineering Co., Ltd
Solve enzyme.
The zymoexciter contains reducing agent and bivalent metal ion.Wherein, the reducing agent is preferably dithiothreitol (DTT)
And/or beta -mercaptoethanol.The bivalent metal ion is preferably selected from Mg2+、Zn2+And Ca2+At least one of, more preferably Mg2 +、Zn2+And Ca2+Mixture, most preferably Mg2+、Zn2+And Ca2+According to molar ratio (0.9~1.1): (0.9~1.1): 1 compounding
Obtained mixture.The bivalent metal ion can exist in the form of the salt containing the above metal ion.It is particularly preferred that
The zymoexciter is by reducing agent and bivalent metal ion and can be with the anion group of the bivalent metal ion forming salt
At.In addition, the molar ratio of reducing agent and bivalent metal ion is particularly preferably 1:(0.9~1.1 in the zymoexciter).
In the present invention, the effect of the ceramic membrane filter be remove the carbohydrate of the ingredient not readily dissolved and the easy moisture absorption at
Point, the water solubility of finally obtained pearl shell peptide finished product is improved, and moisture absorption agglomeration is not easy during finished stock storage.It is inorganic to receive
Ceramic membrane is filtered compared with organic nanofiltration membrane, has organic solvent-resistant, high temperature resistant, acid and alkali-resistance, high mechanical strength, flux big and the service life
Long advantage.The filter core of the inorganic nanofiltration ceramic membrane is preferably using appointing in siliceous, aluminum, magnesia, sa and simatic
A kind of material of anticipating is made.The molecular cut off of the inorganic nanofiltration ceramic membrane is 800Da, when molecular cut off is more than
When 800Da, the carbohydrate content for removing the ingredient not readily dissolved and the easy moisture absorption is not thorough, precious when molecular cut off is less than 800Da
Pearl shellfish peptide loss late is high.
In the present invention, the effect of the high temperature high pressure process is to further increase finally obtained pearl shell peptide finished product
Water solubility easily dissolves so that finished product is finer and smoother, and high-purity pearl shell peptide solution is after high temperature high pressure process,
It can play the role of thoroughly sterilizing and eliminating hypersensitive.The high temperature high pressure process temperature not less than 110 DEG C, pressure it is not low
It is carried out under conditions of 0.1MPa, it is preferable that the condition of the high temperature high pressure process includes that temperature is 110~130 DEG C, and pressure is
0.1~0.3MPa, time are 5~20min.In the present invention, the pressure refers both to gauge pressure.
In the present invention, the effect that the heating under reduced pressure is concentrated is will be in the high-purity pearl shell peptide after high temperature high pressure process
Moist part or all removal.
In the present invention, the effect of the spray drying is that concentrate is processed into powder.Wherein, the spray drying
Temperature can be 110~140 DEG C.
In addition, can also be packed, after formation pearl shell peptide powder to be spray dried to sell outside.
Below with reference to embodiment, the content of the present invention will be explained in more detail.It should be appreciated that the embodiment of the present invention is example
Property, it is intended to it is used to explain the present invention, and is not considered as limiting the invention.Particular technique or item are not specified in embodiment
Part person described technology or conditions or carries out according to the literature in the art according to product description.Agents useful for same or instrument
Production firm person is not specified in device, and being can be with conventional products that are commercially available.
In following embodiment and comparative example, the compound protease, alkali protease, neutral proteinase, flavor protease,
The source of animal proteolytic enzyme and manufacturing enterprise are as shown in table 1 below:
Table 1
| The title of enzyme | Source | Manufacturing enterprise |
| Compound protease | Complex enzyme | Novozymes Company |
| Alkali protease | The lichen bacillus ferments | Nanning Pang Bo bioengineering Co., Ltd |
| Neutral proteinase | Fermentation of bacillus subtilis | Nanning Pang Bo bioengineering Co., Ltd |
| Flavor protease | Aspergillus oryzae fermentation | Nanning Pang Bo bioengineering Co., Ltd |
| Animal proteolytic enzyme | Complex enzyme | Nanning Pang Bo bioengineering Co., Ltd |
Embodiment 1
(1) raw material pre-treatment: by pearl shell decladding, taking pearl oyster meat, and the water of 1 times of raw material weight, group are added after cleaning up
It knits and smashs into homogenate to pieces, heat 10min at 100 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested in the case where not adjusting pH value, the natural conditions for not controlling temperature after uniformly, obtains thick pearl shell peptide solution.The high efficiency
Compounded enzyme preparate is by 20wt% compound protease, 25wt% alkali protease, 20wt% neutral proteinase, 25wt% flavor albumen
Enzyme, 7wt% animal proteolytic enzyme and 3wt% zymoexciter composition, wherein the zymoexciter is by reducing agent dithiothreitol
With bivalent metal ion Mg2+、Zn2+、Ca2+The molar ratio of compounding composition, the reducing agent and bivalent metal ion is 1:0.5,
Mg2+、Zn2+And Ca2+Molar ratio be 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: use molecular cut off for the inorganic of 800Da the thick pearl shell peptide solution
Nanofiltration ceramic membrane filter, gained permeate are high-purity pearl shell peptide solution.
(4) high temperature high pressure process: by the high-purity pearl shell peptide solution temperature is 130 DEG C, pressure is 0.1MPa's
Under the conditions of high temperature high pressure process 20min, obtain treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains pearl shell peptide finished product after packaging.
Embodiment 2
(1) raw material pre-treatment: by pearl shell decladding, taking pearl oyster meat, and the water of 3 times of raw materials weight, group are added after cleaning up
It knits and smashs into homogenate to pieces, heat 20min at 90 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested in the case where not adjusting pH value, the natural conditions for not controlling temperature after uniformly, obtains thick pearl shell peptide solution.The high efficiency
Compounded enzyme preparate is by 22.5wt% compound protease, 22.5wt% alkali protease, 22.5wt% neutral proteinase, 22.5wt%
Flavor protease, 7.5wt% animal proteolytic enzyme and 2.5wt% zymoexciter composition, wherein the zymoexciter is by restoring
Agent dithiothreitol (DTT), beta -mercaptoethanol and bivalent metal ion Mg2+、Zn2+、Ca2+Composition, the reducing agent dithiothreitol
It is 1:1 with the molar ratio of beta -mercaptoethanol, the molar ratio of the reducing agent and bivalent metal ion is 1:1, Mg2+、Zn2+And Ca2+
Molar ratio be 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: use molecular cut off for the inorganic of 800Da the thick pearl shell peptide solution
Nanofiltration ceramic membrane filter, gained permeate are high-purity pearl shell peptide solution.
(4) high temperature high pressure process: by the high-purity pearl shell peptide solution temperature is 120 DEG C, pressure is 0.2MPa's
Under the conditions of high temperature high pressure process 10min, obtain treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains pearl shell peptide finished product after packaging.
Embodiment 3
(1) raw material pre-treatment: by pearl shell decladding, taking pearl oyster meat, and the water of 4 times of raw materials weight, group are added after cleaning up
It knits and smashs into homogenate to pieces, heat 30min at 80 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid.
(2) it digests: toward the high efficiency compounded enzyme preparate to which 0.1% (w/w) raw material weight is added in enzymolysis liquid, mixing
1h is digested in the case where not adjusting pH value, the natural conditions for not controlling temperature after uniformly, obtains thick pearl shell peptide solution.The high efficiency
Compounded enzyme preparate is by 25wt% compound protease, 20wt% alkali protease, 25wt% neutral proteinase, 20wt% flavor albumen
Enzyme, 8wt% animal proteolytic enzyme and 2wt% zymoexciter composition, wherein the zymoexciter is by reducing agent beta -mercaptoethanol
With bivalent metal ion Mg2+、Zn2+、Ca2+The molar ratio of composition, the reducing agent and bivalent metal ion is 1:2, Mg2+、Zn2+
And Ca2+Molar ratio be 1:1:1.
(3) inorganic nanofiltration ceramic membrane filter: use molecular cut off for the inorganic of 800Da the thick pearl shell peptide solution
Nanofiltration ceramic membrane filter, gained permeate are high-purity pearl shell peptide solution.
(4) high temperature high pressure process: by the high-purity pearl shell peptide solution temperature is 110 DEG C, pressure is 0.3MPa's
Under the conditions of high temperature high pressure process 5min, obtain treatment fluid.
(5) it is concentrated: treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate.
(6) dry powder processed and packaging: concentrate is subjected to spray drying powder processed, obtains pearl shell peptide finished product after packaging.
Comparative example 1
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Compound protease in agent is substituted using the animal proteolytic enzyme of identical weight percentage composition, remaining is same as Example 1, obtains
To reference pearl shell peptide finished product.
Comparative example 2
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Alkali protease in agent is substituted using the animal proteolytic enzyme of identical weight percentage composition, remaining is same as Example 1, obtains
To reference pearl shell peptide finished product.
Comparative example 3
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Neutral proteinase in agent is substituted using the animal proteolytic enzyme of identical weight percentage composition, remaining is same as Example 1, obtains
To reference pearl shell peptide finished product.
Comparative example 4
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Flavor protease in agent is substituted using the animal proteolytic enzyme of identical weight percentage composition, remaining is same as Example 1, obtains
To reference pearl shell peptide finished product.
Comparative example 5
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Animal proteolytic enzyme in agent uses the basic protein enzymes extraction of identical weight percentage composition, remaining is same as Example 1, obtains
To reference pearl shell peptide finished product.
Comparative example 6
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, high efficiency compounded enzyme preparate
Contained in zymoexciter completely by bivalent metal ion Mg2+、Zn2+、Ca2+It is 1:1:1 compounding composition according to molar ratio, remaining
It is same as Example 1, obtain reference pearl shell peptide finished product.
Comparative example 7
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, high efficiency compounded enzyme preparate
Contained in zymoexciter be made of completely dithiothreitol (DTT), remaining is same as Example 1, obtains reference pearl shell peptide finished product.
Comparative example 8
Pearl shell peptide is produced according to the method for embodiment 1, unlike, in enzymolysis process, by high efficiency compound enzyme system
Zymoexciter contained in agent is substituted using the animal proteolytic enzyme of identical weight percentage composition, remaining is same as Example 1,
Obtain reference pearl shell peptide finished product.
Test case
(1) high efficiency compounded enzyme preparate hydrolysis result detects:
In Examples 1 to 3 and comparative example 1~8, pearl oyster meat protein under the action of high efficiency compounded enzyme preparate
Do not adjust pH value, do not control temperature under conditions of digested, in order to assess hydrolysis result, after enzymatic hydrolysis, using " middle Chinese
People republic standard GB/T/T 22729-2008 " method in appendix A carries out enzymolysis product detection, as a result such as the following table 2 institute
Show:
Table 2
As shown in Table 2, the enzymolysis product molecular weight of Examples 1 to 3 is small and concentrates, enzymolysis product free amino acid
Content is low (ratio less than 200Da is less than 5%), and ratio of the protein molecular weight less than 1000Da reaches 100%, between 200
The ratio of~1000Da has reached 95% or more, and enzymolysis product molecular weight is substantially better than comparative example 1~8.It is possible thereby to illustrate, adopt
With the combination technique means of heat treatment provided by the invention and high efficiency compounded enzyme preparate enzymatic hydrolysis, it is precious that enzymatic hydrolysis can be significantly improved
The enzymolysis efficiency of pearl shellfish meat protein, so that addition is few in the case where not adjusting optimum pH, not controlling the natural conditions of optimum temperature
Protease is measured, the short period is digested, can thoroughly digest pearl oyster meat protein.
(2) pearl shell peptide finished product physical and chemical index detects:
Physical and chemical index detection is carried out to the pearl shell peptide finished powder that Examples 1 to 3 obtains, wherein total nitrogen detection method
It is carried out referring to GB 5009.5, ash content detection method is carried out referring to GB 5009.4, as a result as shown in table 3 below:
Table 3
| Project | Total nitrogen (in terms of butt) (g/100g) | Ash content (g/100g) |
| Embodiment 1 | 16.1 | 0.8 |
| Embodiment 2 | 15.3 | 0.6 |
| Embodiment 3 | 15.4 | 0.5 |
As shown in Table 3, the total nitrogen for the pearl shell peptide finished product that Examples 1 to 3 obtains, ash content index are lower, say
Bright with high purity using pearl shell peptide obtained by method provided by the invention, quality is good, and especially content of ashes only has 0.5~0.8g/
100g.Illustrate to digest using this specific high efficiency compounded enzyme preparate of the present invention in the case where not adjusting pH value condition, does not need to use
Finished product content of ashes can be greatly reduced so that production process does not introduce inorganic ions in acid-base accommodation pH value.
(3) light transmittance of the water solubility of pearl shell peptide finished product and pearl shell peptide solution detects:
The pearl shell peptide finished powder 8g that Examples 1 to 3 obtains is poured into different cups respectively, with 60 DEG C of 300mL or so
Warm water reconstitutes, and i.e. all dissolutions, obtain pearl shell peptide lysate in the 1s time, visually observes lysate clear, nothing is appointed
What is precipitated.The light transmittance of several pearl shell peptide lysates, as a result as follows more than being detected at 620nm using ultraviolet specrophotometer
Shown in table 4:
Table 4
| Project | Light transmittance (%) |
| Embodiment 1 | 95 |
| Embodiment 2 | 94 |
| Embodiment 3 | 93 |
As shown in Table 4, using the technological means of inorganic nanofiltration ceramic membrane filter, the substance not readily dissolved is removed,
Again using the technological means of the high temperature high pressure process of peptide solution, the solubility property of finished product is further increased, so that the pearl obtained
Shellfish peptide finished product is highly soluble in that water, water solubility are splendid, lysate clear after being dissolved in water, high without any precipitating, light transmittance,
When consumer brews this product, without stirring for it is the technical effect that the prior art is not achieved that convenient, edible experience is good.
(4) the shelf life test of pearl shell peptide finished product:
The pearl shell peptide finished powder 8g that Examples 1 to 3 obtains is poured into packaging bag respectively, room temperature opening is placed in room
On interior Display Rack, visually observes finished product and the time agglomerated occur, as a result as shown in table 5 below:
Table 5
| Project | There is the time (day) agglomerated |
| Embodiment 1 | 49 |
| Embodiment 2 | 52 |
| Embodiment 3 | 51 |
The pearl shell peptide of prior art production is easy moisture absorption agglomeration, influences the sale of product in storage.By table 5
Result it is found that the present invention use inorganic nanofiltration ceramic membrane filter technological means, remove the carbohydrate content of the easy moisture absorption so that at
It is not easy moisture absorption agglomeration in product storage, is easy to store, is the technical effect that the prior art is not achieved.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (8)
1. a kind of production method of pearl shell peptide, which is characterized in that method includes the following steps:
(1) raw material pre-treatment: by pearl shell decladding, taking pearl oyster meat, and the water of 1~4 times of raw material weight, tissue are added after cleaning up
It smashs into homogenate to pieces, heats 10~30min at 80~100 DEG C later, then be cooled to 50~55 DEG C to enzymolysis liquid；
(2) it digests: toward described to which the high efficiency compounded enzyme preparate that dosage is raw material weight 0.05~0.15% is added in enzymolysis liquid,
0.5~2h is digested in the case where not adjusting pH value, the natural conditions for not controlling temperature after mixing, obtains thick pearl shell peptide solution；
The high efficiency compounded enzyme preparate is by 20~25wt% compound protease, 20~25wt% alkali protease, 20~25wt%
Property protease, 20~25wt% flavor protease, 7~8wt% animal proteolytic enzyme and 2~3wt% zymoexciter composition, institute
It states zymoexciter and contains reducing agent and bivalent metal ion；The molar ratio of reducing agent and bivalent metal ion in the zymoexciter
For 1:(0.5~2)；
(3) inorganic nanofiltration ceramic membrane filter: use molecular cut off for the inorganic nanofiltration of 800Da the thick pearl shell peptide solution
Ceramic membrane filter, gained permeate are high-purity pearl shell peptide solution；
(4) high temperature high pressure process: the high-purity pearl shell peptide solution is not less than in temperature not less than 110 DEG C, pressure
High temperature high pressure process is carried out under conditions of 0.1MPa, obtains treatment fluid；
(5) it is concentrated: the treatment fluid being subjected to heating under reduced pressure concentration, obtains concentrate；
(6) dry powder processed: the concentrate is spray-dried, pearl shell peptide powder is obtained.
2. the production method of pearl shell peptide according to claim 1, which is characterized in that in the zymoexciter reducing agent and
The molar ratio of bivalent metal ion is 1:(0.9~1.1).
3. the production method of pearl shell peptide according to claim 1, which is characterized in that the reducing agent is dithiothreitol (DTT)
And/or beta -mercaptoethanol.
4. the production method of pearl shell peptide according to claim 1, which is characterized in that the bivalent metal ion is Mg2+、
Zn2+And Ca2+Mixture.
5. the production method of pearl shell peptide according to claim 4, which is characterized in that Mg2+、Zn2+And Ca2+Molar ratio
For (0.9~1.1): (0.9~1.1): 1.
6. the production method of pearl shell peptide described according to claim 1~any one of 5, which is characterized in that described inorganic
The filter core of nanofiltration ceramic membrane is made using any one material in siliceous, aluminum, magnesia, sa, simatic.
7. the production method of pearl shell peptide described according to claim 1~any one of 5, which is characterized in that the high temperature
The condition of HIGH PRESSURE TREATMENT includes that temperature is 110~130 DEG C, and pressure is 0.1~0.3MPa, and the time is 5~20min.
8. the pearl shell peptide that the method as described in any one of claim 1~7 produces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910236795.9A CN109892661A (en) | 2019-03-27 | 2019-03-27 | A kind of pearl shell peptide and its production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910236795.9A CN109892661A (en) | 2019-03-27 | 2019-03-27 | A kind of pearl shell peptide and its production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109892661A true CN109892661A (en) | 2019-06-18 |
Family
ID=66953191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910236795.9A Withdrawn CN109892661A (en) | 2019-03-27 | 2019-03-27 | A kind of pearl shell peptide and its production method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109892661A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115039828A (en) * | 2022-06-30 | 2022-09-13 | 集美大学 | Pearl mussel peptide with effect of dispelling effects of alcohol and preparation method thereof |
| CN115444924A (en) * | 2022-09-29 | 2022-12-09 | 广东海洋大学 | Pearl oyster protein peptide composition and preparation and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101100689A (en) * | 2007-08-07 | 2008-01-09 | 海南京润珍珠生物技术有限公司 | Method for preparing pearl active peptide |
| CN101341978A (en) * | 2008-08-13 | 2009-01-14 | 赵兴坤 | Holothurian peptide functional food and preparation method thereof |
| CN101701241A (en) * | 2009-11-05 | 2010-05-05 | 山东好当家海洋发展股份有限公司 | Preparation method of scallop active peptide |
| CN101892280A (en) * | 2010-06-29 | 2010-11-24 | 中国水产科学研究院南海水产研究所 | Preparation method of pinctada fucata martensii meat anti-oxidizing oligopeptides |
| CN101982114A (en) * | 2010-09-26 | 2011-03-02 | 广东海洋大学 | Method for producing anti-fatigue oral liquids by using Pinctada martensii dunker meats |
| CN102220402A (en) * | 2011-05-27 | 2011-10-19 | 中国水产科学研究院南海水产研究所 | Method for extracting antibacterial peptide from pearl oyster meat |
| CN106282284A (en) * | 2016-08-11 | 2017-01-04 | 大连豪翔生物酶工程有限公司 | Stichopus japonicus oligopeptide production enzyme and enzymolysis process |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
-
2019
- 2019-03-27 CN CN201910236795.9A patent/CN109892661A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101100689A (en) * | 2007-08-07 | 2008-01-09 | 海南京润珍珠生物技术有限公司 | Method for preparing pearl active peptide |
| CN101341978A (en) * | 2008-08-13 | 2009-01-14 | 赵兴坤 | Holothurian peptide functional food and preparation method thereof |
| CN101701241A (en) * | 2009-11-05 | 2010-05-05 | 山东好当家海洋发展股份有限公司 | Preparation method of scallop active peptide |
| CN101892280A (en) * | 2010-06-29 | 2010-11-24 | 中国水产科学研究院南海水产研究所 | Preparation method of pinctada fucata martensii meat anti-oxidizing oligopeptides |
| CN101982114A (en) * | 2010-09-26 | 2011-03-02 | 广东海洋大学 | Method for producing anti-fatigue oral liquids by using Pinctada martensii dunker meats |
| CN102220402A (en) * | 2011-05-27 | 2011-10-19 | 中国水产科学研究院南海水产研究所 | Method for extracting antibacterial peptide from pearl oyster meat |
| CN106282284A (en) * | 2016-08-11 | 2017-01-04 | 大连豪翔生物酶工程有限公司 | Stichopus japonicus oligopeptide production enzyme and enzymolysis process |
| CN109371089A (en) * | 2018-12-25 | 2019-02-22 | 河北肽都生物科技有限公司 | A kind of extracting method of small molecule liver peptide |
Non-Patent Citations (3)
| Title |
|---|
| 夏克胜等: "米曲霉粗酶和商业蛋白酶对马氏珍珠贝肉酶解特性的比较", 《食品与发酵工业》 * |
| 张晓瑜等: "陶瓷膜微滤对马氏珠母贝肉酶解液理化特性的影响", 《食品工业科技》 * |
| 蒲月华等: "珍珠贝多肽体外抗氧化活性的研究 ", 《食品科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115039828A (en) * | 2022-06-30 | 2022-09-13 | 集美大学 | Pearl mussel peptide with effect of dispelling effects of alcohol and preparation method thereof |
| CN115444924A (en) * | 2022-09-29 | 2022-12-09 | 广东海洋大学 | Pearl oyster protein peptide composition and preparation and application thereof |
| CN115444924B (en) * | 2022-09-29 | 2023-03-10 | 广东海洋大学 | Pearl oyster protein peptide composition and preparation and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109735591A (en) | A kind of ox bone collagen protein peptides and its production method | |
| WO2021142880A1 (en) | Method for producing clam active peptide | |
| CN109907327A (en) | A kind of abalone peptide and its production method | |
| CN109722461A (en) | A kind of deep-sea fish collagen peptide and its production method | |
| CN104830936A (en) | Hypoallergenic low fishy smell fish protein oligopeptide, and industrial preparation method and application thereof | |
| CN109897878A (en) | A kind of oyster peptide and its production method and application | |
| CN110024901A (en) | A kind of collagen peptide and its production method and process units and application | |
| CN101341978A (en) | Holothurian peptide functional food and preparation method thereof | |
| CN109897101B (en) | Collagen tripeptide and production method and application thereof | |
| CN105925649B (en) | Preparation method of low-molecular-weight degreased squid protein functional active peptide | |
| CN102550797A (en) | Production method of sea cucumber peptide extract | |
| CN108103131A (en) | A kind of preparation process of ocean fish collagen oligopeptide powder and its application | |
| WO2018059245A1 (en) | Pure collagen milk powder and preparation method thereof | |
| CN102766670A (en) | Preparation method of oyster polypeptide powder | |
| CN112790392A (en) | Preparation method of sea cucumber peptide | |
| CN106047975A (en) | Mussel protein peptide extraction method | |
| CN109892661A (en) | A kind of pearl shell peptide and its production method | |
| CN109182432A (en) | The preparation method of fish collagen oligopeptide | |
| CN103571900A (en) | Preparation and efficacy of low molecular weight soybean peptide | |
| CN105011187A (en) | Processing method for novel hot pot tripe | |
| CN109797184A (en) | A kind of crocodile peptide and its production method | |
| JPH05123119A (en) | Production of transparent royal jelly solution | |
| CN109757601B (en) | Sea cucumber peptide and production method thereof | |
| CN101619095B (en) | Preparation method of soybean polysaccharide protein in soybean dregs | |
| CN102511890A (en) | Jellyfish beverage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20190618 |