CN111700606A

CN111700606A - Microelectrode preparation method, tattooing machine, microelectrode and signal acquisition device

Info

Publication number: CN111700606A
Application number: CN202010524427.7A
Authority: CN
Inventors: 蓝河; 李胜夏
Original assignee: Shanghai Mi Fang Electronics Ltd
Current assignee: Shanghai Mi Fang Electronics Ltd
Priority date: 2020-06-10
Filing date: 2020-06-10
Publication date: 2020-09-25

Abstract

The invention discloses a microelectrode preparation method, a tattooing machine, a microelectrode and a signal acquisition device, wherein the microelectrode is used for acquiring an electric signal of an object to be detected, and the method comprises the following steps: receiving a preparation instruction of a microelectrode; responding to the preparation instruction, and controlling a micro-needle carrying conductive liquid to penetrate into a signal acquisition position of the object to be detected; and controlling the micro-needle to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the tail end of the micro-needle with a set depth from the surface layer of the object to be detected, wherein the micro-needle moves for a set distance between the first position and the second position at set intervals, and injecting the conductive liquid until the micro-needle moves to the surface layer of the object to be detected. The conductive component in the conductive liquid is integrated with the skin, and the part injected with the conductive liquid can form a microelectrode which is fixed on the epidermis layer of the skin and is used for collecting the electric signal of the epidermis layer, so that the collected electric signal is more stable and accurate.

Description

Microelectrode preparation method, tattooing machine, microelectrode and signal acquisition device

Technical Field

The invention relates to the technical field of biological detection, in particular to a microelectrode preparation method, a tattooing machine, a microelectrode and a signal acquisition device.

Background

In recent years, with the continuous development and maturity of Micro-Electro-Mechanical Systems (MEMS) technology, the application range of Micro-sensors is continuously expanded, and the Micro-sensors are widely applied to the fields of medicine, environmental detection and the like, and the research on the needle type Micro-electrode based on the Micro-processing technology is rapidly developed.

The micro-needle electrode effectively solves the problem of high impedance of skin and realizes acquisition of bioelectric signals, but the micro-needle electrode is mainly prepared by semiconductor processes such as photoetching, etching and the like at present, and after the preparation is finished, the micro-needle electrode is penetrated into the skin to collect related electric signals in the using process of the micro-needle electrode. The method has the defects of complicated process steps, high cost, complex flow, low flexibility of structural design and application and the like.

Disclosure of Invention

In order to solve the problems existing in the preparation and use processes of the microneedle electrode, the embodiment of the invention creatively provides a microelectrode preparation method, a tattooing machine, a microelectrode and a signal acquisition device.

According to a first aspect of the present invention, there is provided a method for preparing a microelectrode, the microelectrode being used for acquiring an electrical signal of an object to be detected, the method comprising: receiving a preparation instruction of a microelectrode; responding to the preparation instruction, and controlling a micro-needle carrying conductive liquid to penetrate into a signal acquisition position of the object to be detected; and controlling the micro-needle to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the tail end of the micro-needle, which is a set depth from the surface layer of the object to be detected, wherein the micro-needle moves a set distance between the first position and the second position at set intervals, and the conductive liquid is injected until the micro-needle moves to the surface layer of the object to be detected.

According to an embodiment of the invention, the method further comprises: after the conductive liquid is injected to the signal acquisition position every time, heating and annealing the object to be detected; and/or heating and annealing the object to be detected when the micro-needle moves to the surface layer of the object to be detected.

According to one embodiment of the present invention, a method for controlling a signal collecting position of a conductive liquid carried microneedle to penetrate into an object to be detected includes: determining second relative positions of a plurality of microneedles with the same number as the signal acquisition positions according to the first relative positions of the signal acquisition positions; and simultaneously puncturing a plurality of micro-needles into corresponding signal acquisition positions according to the second relative position.

According to an embodiment of the present invention, the set depth is a thickness of an epidermal layer of the object to be detected.

According to an embodiment of the present invention, the conductive liquid includes at least one of: metal nanoparticles, conductive polymers, conductive hydrogels.

According to a second aspect of embodiments of the present invention, there is also provided a tattooing machine for manufacturing micro-electrodes according to the above-described micro-electrode manufacturing method.

According to a third aspect of the present embodiment, there is also provided a micro-electrode produced according to the above-mentioned micro-electrode production method.

According to a fourth aspect of the embodiments of the present invention, there is also provided a signal collecting device for collecting an electrical signal of an object to be detected, the device including a plurality of microelectrodes prepared by the above method for preparing microelectrodes.

According to an embodiment of the invention, the apparatus further comprises: and the patch electrode is used for being attached to the surface layer of the object to be detected and is electrically connected with one end of the microelectrode, which is positioned on the surface layer of the object to be detected.

According to an embodiment of the invention, the patch electrode is prepared by using a 3D printing circuit or microelectronic printing technology.

According to the microelectrode preparation method, the tattooing machine, the microelectrode and the signal acquisition device provided by the embodiment of the invention, the conductive liquid is injected into the epidermal layer of the object to be detected through the microneedle, for example: the conductive liquid can be dried by body temperature or by auxiliary heating of a heat source, so that the conductive component in the conductive liquid and the skin are integrated, a microelectrode can be formed at the part where the conductive liquid is injected, and the microelectrode is fixed on the skin epidermis and can be used for collecting various electrical signals in the epidermis. And the microelectrode is fixed on the epidermis layer of the skin, so that the acquired electric signal is more stable and accurate.

It is to be understood that the teachings of the present invention need not achieve all of the above-described benefits, but rather that specific embodiments may achieve specific technical results, and that other embodiments of the present invention may achieve benefits not mentioned above.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present invention will become readily apparent from the following detailed description read in conjunction with the accompanying drawings. Several embodiments of the invention are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

in the drawings, the same or corresponding reference numerals indicate the same or corresponding parts.

FIG. 1 is a schematic view showing an application scene of a micro-electrode according to an embodiment of the present invention;

FIG. 2 is a schematic view showing an implementation flow of a method for producing a micro-electrode according to an embodiment of the present invention.

Detailed Description

The principles and spirit of the present invention will be described with reference to a number of exemplary embodiments. It is understood that these embodiments are given only to enable those skilled in the art to better understand and to implement the present invention, and do not limit the scope of the present invention in any way. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

The technical solution of the present invention is further elaborated below with reference to the drawings and the specific embodiments.

FIG. 1 is a schematic view showing an application scene of a micro-electrode according to an embodiment of the present invention.

Referring to fig. 1, the microelectrode 10 is used for collecting an electrical signal of an object to be detected, which may be a human or an animal, or other objects, such as: a microelectrode 10 is implanted into a solid block prepared from a new material to acquire electrical signals such as voltage, current, resistance or communication signals. The embodiment of the present invention will be described by taking an object to be detected as a human or an animal as an example. As shown in fig. 1, according to the method for manufacturing a microelectrode provided by the embodiment of the present invention, a microneedle capable of carrying a conductive liquid is used to pierce a designated position of an object to be detected, and the conductive liquid is injected into the designated position, and after the injected conductive liquid is subjected to heating annealing or other process operations, a columnar body can be formed, and in practical applications, a conical body and the like can be formed according to different components of the conductive liquid and requirements on signal acquisition accuracy of the object to be detected. The microneedle is inserted into the designated position, which may be directly inserted into the critical position of the epidermis layer 102 and the dermis layer 103 of the subject, and then the microneedle is controlled to continuously inject the conductive liquid in the process of moving towards the surface layer 101, and the conductive liquid may dry under the action of the body temperature of the subject, for example: the conductive liquid dries under the action of the body temperature to form the microelectrode 10. The step of inserting the microneedles into the designated positions can also be that the microneedles are inserted into a position which is a set distance away from the surface layer 101 between the critical positions of the epidermis layer 102 and the dermis layer 103 of the object to be detected.

In one embodiment of the present invention, the conductive liquid may be implanted into the human epidermis layer 102 using microneedles of a tattooing machine according to the principle that the tattooing machine implants the pigment ink into the human epidermis layer 102. Or a microelectrode manufacturing device designed according to actual needs. In the embodiments of the present invention, the apparatus for producing the micro-electrode 10 is referred to as a micro-electrode manufacturing apparatus, unless otherwise specified.

Referring to fig. 2, a method for preparing a micro-electrode according to an embodiment of the present invention includes at least: an operation 201 of receiving a preparation instruction of a microelectrode; an operation 202, responding to the preparation instruction, and controlling a micro-needle carrying conductive liquid to penetrate into a signal acquisition position of the object to be detected; and operation 203, controlling the micro-needle to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the tail end of the micro-needle with a set depth from the surface layer of the object to be detected, moving the micro-needle between the first position and the second position at a set interval for a set distance, and injecting a conductive liquid until the micro-needle moves to the surface layer of the object to be detected.

In operation 201, a preparation instruction of a micro-electrode is received.

In one embodiment of the present invention, the preparation instruction of the micro-electrode may be a start signal of a micro-electrode manufacturing apparatus, for example: power switch, start button, etc. The mode selection button may be a physical button or a virtual button provided on a screen display device of the microelectrode manufacturing device.

For example, a tattooing machine is used for preparing a microelectrode, the tattooing machine which can automatically control the depth of the skin of a human body and is provided with a plurality of microneedles can set the depth of the skin of the human body and adjust the relative distance between the microneedles according to actual requirements, and after the setting is finished, a microelectrode preparation instruction is sent out, for example: click a start button, etc.

In operation 202, in response to the preparation instruction, a microneedle carrying a conductive liquid is controlled to penetrate into a signal acquisition position of the object to be detected.

In one embodiment of the present invention, the conductive liquid includes at least one of: metal nanoparticles, conductive polymers, conductive hydrogels.

For example, the conductive liquid may be a metal nanoparticle ink, a conductive polymer ink. The ink may be transparent ink or colored ink, and may be selected according to actual requirements. For example: for the electric signal collection of the head or the heart and other parts of the human body, the signal collection position is positioned at the clothes covering part, the area of the microelectrode exposed on the skin surface is extremely small, the attractiveness is not influenced, and colored ink can be adopted, for example: black, etc. Therefore, when the electric signal acquisition is needed, the position of the microelectrode can be conveniently determined, and when the corresponding microelectrode is not needed any more, the microelectrode can be conveniently found and taken out by adopting a proper method.

In one embodiment of the present invention, a microneedle carrying a conductive liquid may be inserted into a signal collecting position of an object to be detected. For example, a microneedle carrying a metallic nanoparticle ink is inserted into the epidermis layer of the human head.

In an embodiment of the present invention, a plurality of conductive liquid-carrying microneedles may be inserted into the signal collecting position of the object to be detected, and specifically, the following method may be adopted to control the conductive liquid-carrying microneedles to penetrate into the signal collecting position of the object to be detected: determining second relative positions of a plurality of microneedles with the same number as the signal acquisition positions according to the first relative positions of the signal acquisition positions; and simultaneously puncturing a plurality of micro-needles into the corresponding signal acquisition positions according to the second relative position.

For example, a micro-electrode may be provided on the head of a person who needs to detect electrical signals such as brain waves for a long period of time. The head has a plurality of signal acquisition positions, the number of the plurality of signal acquisition positions and a first relative position between the plurality of signal acquisition positions being determined. The number of the first relative positions and the number of the signal acquisition positions can be input into a control device of the microelectrode preparation equipment, so that the microneedles with the same number as the signal acquisition positions can be automatically adjusted according to the number of the signal acquisition positions, and the relative positions of the microneedles can be automatically adjusted according to the first relative positions.

In an embodiment of the present invention, the preparation instruction carrying the signal collecting position of the object to be detected may first receive the signal collecting position of the object to be detected, and then control the microneedle carrying the conductive liquid to move to the signal collecting position according to the signal collecting position carried in the preparation instruction.

In operation 203, the microneedle is controlled to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the end of the microneedle, which is a set depth from the surface layer of the object to be detected, and the microneedle moves a set distance between the first position and the second position at a set interval and injects the conductive liquid until the microneedle moves to the surface layer of the object to be detected.

In one embodiment of the present invention, the depth is set to the thickness of the epidermis layer of the object to be detected.

For example, the object to be detected is a human body, and it is necessary to acquire an electrical signal from a certain part of the human body. The microelectrode can be prepared to the epidermis layer of human skin, one end of the microelectrode can be arranged on the epidermis layer of human skin, and the other end of the microelectrode can be arranged at the critical position of the epidermis layer and the dermis layer of human body. Thus, the prepared microelectrode can be used for better collecting the electric signals near the dermis layer of the human skin.

In an embodiment of the present invention, the controlling of the microneedle to move between the first position of the surface layer of the object to be detected corresponding to the signal collecting position and the second position of the distal end of the microneedle having a set depth from the surface layer of the object to be detected may include the following two moving manners:

1. firstly, the micro-needle is controlled to directly penetrate into a second position, corresponding to the signal acquisition position, of a set depth from the tail end of the micro-needle to the surface layer of the object to be detected, and then the micro-needle continuously moves to a first position, corresponding to the signal acquisition position, of the surface layer of the object to be detected.

2. Firstly, a microneedle is controlled to pierce a first position of a surface layer of an object to be detected corresponding to a signal acquisition position, then the microneedle continuously moves to a second position, with a set depth from the tail end of the microneedle corresponding to the signal acquisition position to the surface layer of the object to be detected, and after the microneedle finishes the injection of a conductive liquid, the microneedle moves to the first position from the second position.

In an actual application process, other suitable manners may also be adopted to control the microneedle to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the distal end of the microneedle, which is a set depth from the surface layer of the object to be detected.

Hereinafter, a description will be given of "the microneedles are moved at a predetermined distance between the first and second positions at predetermined intervals and the conductive liquid is injected" by taking an example in which the microneedles are directly inserted into the second position and are continuously moved to the first position.

In an embodiment of the present invention, the microneedle moves a set distance between the first position and the second position at a set time interval, and injects the conductive liquid, which may be at a position where the distal end of the microneedle is a set depth from the surface layer of the object to be detected, and the microneedle keeps the relative position with the object to be detected unchanged, and injects the conductive liquid with a set weight or a set volume. And after moving the surface layer of the object to be detected for a set distance at set time intervals, executing the same operation, keeping the relative position of the micro-needle and the object to be detected unchanged, and injecting a set weight or a set volume of conductive liquid. The setting time and the setting distance of the micro-needle movement process can be set according to actual needs, and the electric conductor formed after the conductive liquid is dried can ensure that the electric signal to be acquired is transmitted to the surface layer of the object to be acquired from the set depth from the surface layer of the object to be acquired at the signal acquisition position. Thus, the conductive liquid can form a column from a set depth to the surface layer at the signal acquisition position.

In an embodiment of the present invention, when the microneedle moves to the surface layer of the object to be detected for a set distance at a set time interval, the set time approaches zero, and the set distance also approaches zero, which can be regarded as that the microneedle moves to the surface layer of the object to be detected from a set depth from the surface layer of the object to be detected at a constant speed, and injects the conductive liquid to the signal acquisition position at a constant speed during the movement. Thus, the conductive liquid can form a column from a set depth to the surface layer at the signal acquisition position.

In one embodiment of the present invention, in the process that the microneedles move to the surface layer of the object to be detected for a set distance at a set time interval, the set time is gradually increased, the set distance is also gradually decreased, and the conductive liquid is uninterruptedly injected to the signal acquisition position in the moving process of the microneedles, so that a conical body with a diameter gradually increased from a set depth from the surface layer of the object to be acquired to the surface layer of the object to be acquired can be formed at the signal acquisition position. In contrast, in an embodiment of the present invention, when the microneedles move to the surface layer of the object to be detected at a set time interval by a set distance, the set time is gradually decreased, the set distance is also gradually increased, and the conductive liquid is continuously injected into the signal collection position during the movement of the microneedles, so that a tapered body whose diameter gradually decreases from the set depth from the surface layer of the object to be collected to the surface layer of the object to be collected can be formed at the signal collection position.

In an embodiment of the present invention, during the process of injecting the conductive liquid into the signal collecting position, and after the micro-needle is moved to the surface layer of the detection object to complete the injection of all the conductive liquid, the conductive liquid is naturally dried to form the micro-electrode. For example: in the process of preparing the microelectrode, the environment temperature of the object to be detected is higher, and the conductive liquid is gradually dried by depending on the environment temperature and the temperature of the object to be detected, so that the microelectrode is formed.

It should be noted that, the shape of the finally formed microelectrode, which is influenced by the image of the factors such as the moving speed of the microneedle in the moving process, the uniformity of the conductive liquid in the injecting process, and the like, may be a cylinder, a cone, a polyhedral cylinder, or other regular shapes or irregular shapes, as long as a continuous conductor can be formed from the surface layer of the object to be detected to the position away from the surface layer of the object to be detected by the set depth, and the specific shape is not limited in the embodiments of the present invention.

In one embodiment of the present invention, after the conductive liquid is injected to the signal collection position each time, the object to be detected is also subjected to heating annealing; and/or heating and annealing the object to be detected only when the micro-needle moves to the surface layer of the object to be detected.

For example, after the micro-needle sends the conductive liquid (e.g., the metal nano conductive ink) to a designated position of the skin (which may be various parts of a human body or other living bodies, such as the scalp), the conductive liquid is dried by the body temperature or annealed by an external auxiliary heat source, and the micro-electrode can be conducted and conducted from the dermis to the skin surface by injecting the ink (also referred to as "printing") at different depths during the process of moving the micro-needle from a set depth to the surface of the object to be collected (e.g., moving from the dermis of the human scalp to the skin surface). The external auxiliary heat source may be a hot air or a static heater, and the temperature of the hot air or the static heater needs to be set according to the temperature that the object to be detected can bear.

Thus, in the microelectrode preparation method, the tattooing machine, the microelectrode and the signal acquisition device according to the embodiments of the present invention, the conductive liquid is injected into the epidermal layer of the object to be detected through the microneedle, for example: the conductive liquid can be dried by body temperature or by auxiliary heating of a heat source, so that the conductive component in the conductive liquid and the skin are integrated, a microelectrode can be formed at the part where the conductive liquid is injected, and the microelectrode is fixed on the skin epidermis and can be used for collecting various electrical signals in the epidermis. And the microelectrode is fixed on the epidermis layer of the skin, so that the acquired electric signal is more stable and accurate. The preparation process of the microelectrode is greatly simplified, and the preparation efficiency of the microelectrode is effectively improved. In addition, the embodiment of the invention can implant the microelectrode into the signal acquisition position of the object to be detected while rapidly preparing the microelectrode, and simultaneously execute the preparation and implantation steps of the microelectrode, thereby effectively simplifying the preparation and use processes of the microelectrode.

In the same way, based on the above microelectrode preparation method, the embodiment of the invention also provides a tattooing machine, and the tattooing machine is used for preparing the microelectrode according to the microelectrode preparation method.

Further, based on the above method for preparing a microelectrode, the embodiment of the invention also provides a microelectrode, and the microelectrode is prepared according to the method for preparing the microelectrode.

Furthermore, based on the above method for preparing a microelectrode, the embodiment of the present invention further provides a signal collecting device for collecting an electrical signal of an object to be detected, wherein the device comprises a plurality of microelectrodes prepared by the above method for preparing microelectrodes.

According to an embodiment of the present invention, the signal acquisition apparatus further includes: and the patch electrode is used for being attached to the surface layer of the object to be detected and is electrically connected with one end of the microelectrode, which is positioned on the surface layer of the object to be detected.

Here, it should be noted that: the above descriptions of the embodiments of the tattooing machine, the micro-electrodes, and the signal collecting device are similar to the description of the embodiment of the method shown in fig. 1-2, and have similar advantageous effects to the embodiment of the method shown in fig. 1-2, and therefore are not repeated. For technical details which are not disclosed in the embodiments of the tattooing machine, the microelectrode and the signal acquisition device according to the present invention, please refer to the description of the method embodiments shown in fig. 1 to 2 for understanding, and therefore, for brevity, will not be described again.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

In the several embodiments provided in the present application, it should be understood that the disclosed apparatus and method may be implemented in other ways. The above-described device embodiments are merely illustrative, for example, the division of a unit is only one logical function division, and there may be other division ways in actual implementation, such as: multiple units or components may be combined, or may be integrated into another system, or some features may be omitted, or not implemented. In addition, the coupling, direct coupling or communication connection between the components shown or discussed may be through some interfaces, and the indirect coupling or communication connection between the devices or units may be electrical, mechanical or other forms.

The units described as separate parts may or may not be physically separate, and parts displayed as units may or may not be physical units; can be located in one place or distributed on a plurality of network units; some or all of the units can be selected according to actual needs to achieve the purpose of the solution of the embodiment.

In addition, all the functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may be separately regarded as one unit, or two or more units may be integrated into one unit; the integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional unit.

Those of ordinary skill in the art will understand that: all or part of the steps for realizing the method embodiments can be completed by hardware related to program instructions, the program can be stored in a computer readable storage medium, and the program executes the steps comprising the method embodiments when executed; and the aforementioned storage medium includes: various media that can store program codes, such as a removable Memory device, a Read Only Memory (ROM), a magnetic disk, or an optical disk.

Alternatively, the integrated unit of the present invention may be stored in a computer-readable storage medium if it is implemented in the form of a software functional module and sold or used as a separate product. Based on such understanding, the technical solutions of the embodiments of the present invention may be essentially implemented or a part contributing to the prior art may be embodied in the form of a software product, which is stored in a storage medium and includes several instructions for enabling a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the methods of the embodiments of the present invention. And the aforementioned storage medium includes: a removable storage device, a ROM, a magnetic or optical disk, or other various media that can store program code.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A microelectrode preparation method is used for acquiring an electric signal of an object to be detected, and is characterized by comprising the following steps:

receiving a preparation instruction of a microelectrode;

responding to the preparation instruction, and controlling a micro-needle carrying conductive liquid to penetrate into a signal acquisition position of the object to be detected;

and controlling the micro-needle to move between a first position of the surface layer of the object to be detected corresponding to the signal acquisition position and a second position of the tail end of the micro-needle, which is a set depth from the surface layer of the object to be detected, wherein the micro-needle moves a set distance between the first position and the second position at set intervals, and the conductive liquid is injected until the micro-needle moves to the surface layer of the object to be detected.

2. The method of claim 1, further comprising:

after the conductive liquid is injected to the signal acquisition position every time, heating and annealing the object to be detected; and/or

And when the micro-needle moves to the surface layer of the detection object, heating and annealing the object to be detected.

3. The method of claim 1, wherein controlling the signal acquisition position of the conductive liquid carrying microneedle penetrating into the object to be detected comprises:

determining second relative positions of a plurality of microneedles with the same number as the signal acquisition positions according to the first relative positions of the signal acquisition positions;

and simultaneously puncturing a plurality of micro-needles into corresponding signal acquisition positions according to the second relative position.

4. The method according to claim 1, wherein the set depth is a thickness of an epidermal layer of the object to be detected.

5. The method of claim 1, wherein the conductive liquid comprises at least one of: metal nanoparticles, conductive polymers, conductive hydrogels.

6. A tattooing machine, wherein said tattooing machine is used for manufacturing a microelectrode according to the method for manufacturing a microelectrode according to any one of claims 1 to 5.

7. A microelectrode, characterized in that it is produced according to the method for producing a microelectrode according to any of claims 1 to 5.

8. A signal acquisition device for acquiring an electric signal of an object to be detected, wherein the device comprises a plurality of microelectrodes prepared by the method for preparing a microelectrode according to any one of claims 1 to 5.

9. The signal acquisition device of claim 8, wherein the device further comprises:

and the patch electrode is used for being attached to the surface layer of the object to be detected and is electrically connected with one end of the microelectrode, which is positioned on the surface layer of the object to be detected.

10. The device of claim 9, wherein the patch electrodes are fabricated using 3D printing circuitry or microelectronic printing technology.