CN111700149A - Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae - Google Patents
Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae Download PDFInfo
- Publication number
- CN111700149A CN111700149A CN202010616751.1A CN202010616751A CN111700149A CN 111700149 A CN111700149 A CN 111700149A CN 202010616751 A CN202010616751 A CN 202010616751A CN 111700149 A CN111700149 A CN 111700149A
- Authority
- CN
- China
- Prior art keywords
- radix puerariae
- purple
- purple radix
- selenium
- active ingredients
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 239000011669 selenium Substances 0.000 title claims abstract description 37
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 19
- 239000004480 active ingredient Substances 0.000 title claims abstract description 18
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 18
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 69
- 239000000843 powder Substances 0.000 claims abstract description 28
- 238000000502 dialysis Methods 0.000 claims abstract description 25
- 239000002244 precipitate Substances 0.000 claims abstract description 14
- 239000006228 supernatant Substances 0.000 claims abstract description 13
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 12
- 239000007853 buffer solution Substances 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 238000000643 oven drying Methods 0.000 claims abstract description 6
- 238000010298 pulverizing process Methods 0.000 claims abstract description 6
- 238000005119 centrifugation Methods 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 5
- 241000731396 Pueraria montana var. thomsonii Species 0.000 claims description 2
- 239000003054 catalyst Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 23
- 102000008114 Selenoproteins Human genes 0.000 abstract description 13
- 108010074686 Selenoproteins Proteins 0.000 abstract description 13
- 235000006708 antioxidants Nutrition 0.000 description 11
- 238000005303 weighing Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- ZQSIJRDFPHDXIC-UHFFFAOYSA-N Daidzein Natural products C1=CC(O)=CC=C1C1=COC2=CC(O)=CC=C2C1=O ZQSIJRDFPHDXIC-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 241000723353 Chrysanthemum Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- GMTUGPYJRUMVTC-UHFFFAOYSA-N Daidzin Natural products OC(COc1ccc2C(=O)C(=COc2c1)c3ccc(O)cc3)C(O)C(O)C(O)C=O GMTUGPYJRUMVTC-UHFFFAOYSA-N 0.000 description 1
- KYQZWONCHDNPDP-UHFFFAOYSA-N Daidzoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-UHFFFAOYSA-N 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000219780 Pueraria Species 0.000 description 1
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- QXMNTPFFZFYQAI-IMDKZJJXSA-N beta-sitosterol 3-O-beta-D-glucopyranoside Natural products CC[C@H](CC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@H](CC[C@]4(C)[C@H]3CC[C@]12C)O[C@@H]5C[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)C(C)C QXMNTPFFZFYQAI-IMDKZJJXSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 235000007240 daidzein Nutrition 0.000 description 1
- KYQZWONCHDNPDP-QNDFHXLGSA-N daidzein 7-O-beta-D-glucoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C2C(=O)C(C=3C=CC(O)=CC=3)=COC2=C1 KYQZWONCHDNPDP-QNDFHXLGSA-N 0.000 description 1
- NPJICTMALKLTFW-OFUAXYCQSA-N daucosterol Chemical compound O([C@@H]1CC2=CC[C@H]3[C@@H]4CC[C@@H]([C@]4(CC[C@@H]3[C@@]2(C)CC1)C)[C@H](C)CC[C@@H](CC)C(C)C)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O NPJICTMALKLTFW-OFUAXYCQSA-N 0.000 description 1
- QDFKFNAHVGPRBL-UHFFFAOYSA-N daucosterol Natural products CCC(CCC(C)C1CCC2C1CCC3C2(C)CC=C4CC(CCC34C)OC5OC(CO)C(O)C(O)C5O)C(C)C QDFKFNAHVGPRBL-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 150000002337 glycosamines Chemical class 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000015961 tonic Nutrition 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 229960000716 tonics Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of plant extraction, in particular to a method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae. A method for extracting selenium-containing antioxidant active ingredients from purple radix Puerariae comprises the following steps: (1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder; (2) adding NaOH into the purple radix puerariae powder, magnetically stirring at room temperature, centrifuging, and taking supernatant; (3) adding HCl to the supernatant to adjust pH, and then adding (NH)4)2SO4Standing in a refrigerator for 8-10h, centrifuging, and collecting precipitate; (4) adding NaOH solution into the precipitate to dissolve, and transferring into dialysis bag; (5) placing the dialysis bag in a buffer solution, and placing the dialysis bag togetherDialyzing in refrigerator for 18-20 hr, and vacuum freeze drying. The extraction method can improve the extraction rate of the selenoprotein in the purple radix puerariae, guides the eating amount of the purple radix puerariae, and further improves the utilization value of the purple radix puerariae.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to a method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae.
Background
Purple radix Puerariae is a plant of Pueraria of Leguminosae. Radix Puerariae contains starch as main ingredient, and about 12% of flavonoids including more than 10 kinds of daidzin, daidzein and puerarin, and contains daucosterol, amino acids and coumarins; and protein, amino acid, sugar and minerals such as calcium, iron, copper, selenium and the like which are necessary for human bodies, are good and precious tonics for both the old and the young, and have the reputation of 'thousand years ginseng'. Particularly, the trace element selenium contained in the purple radix puerariae is one of four antioxidants and is also a trace element necessary for human bodies, the trace element selenium can enhance the scavenging capacity of free radicals, improve the oxidation resistance in vivo, improve the immunity, reduce blood fat, resist tumors, prevent senile diseases and the like, and the selenium intake of 50-250mg per day is recommended by the Chinese academy of nutrition. Because purple radix puerariae contains abundant trace element selenium, people can meet the daily intake of selenium by eating purple radix puerariae. However, the selenium content in the purple radix puerariae is different due to the influence of objective conditions such as planting areas, planting environments and the like, and the eating amount does not have a uniform reference standard, so that the selenium content in the purple radix puerariae needs to be extracted and measured so as to provide guidance for the eating amount of the purple radix puerariae.
Application number CN201910700446.8 discloses a method for extracting selenium-containing antioxidant active ingredients from selenium-rich chrysanthemum, but the method is designed for the selenium-rich chrysanthemum. When the inventor adopts the method to extract the selenium-containing antioxidant active ingredients in the purple pachyrhizua angulatus, the extraction rate is low.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae, which is designed for the purple radix puerariae, can improve the extraction rate of selenium protein in the purple radix puerariae, guides the eating amount of the purple radix puerariae, and further improves the utilization value of the purple radix puerariae.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for extracting selenium-containing antioxidant active ingredients from purple radix Puerariae comprises the following steps:
(1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder;
(2) adding NaOH into the purple radix puerariae powder, magnetically stirring at room temperature, centrifuging, and taking supernatant;
(3) adding HCl to the supernatant to adjust pH, and then adding (NH)4)2SO4Standing in a refrigerator for 8-10h, centrifuging, and collecting precipitate;
(4) adding NaOH solution into the precipitate to dissolve, and transferring into dialysis bag;
(5) and (3) putting the dialysis bag into the buffer solution, putting the dialysis bag into a refrigerator for dialysis for 18-20h, and finally performing vacuum freeze drying.
Preferably, the purple radix Puerariae powder in step (1) can pass through 60-80 mesh sieve.
Preferably, the concentration of NaOH in the step (2) is 0.15mol/L, and the feed-liquid ratio of the purple radix puerariae powder to the NaOH is 1: 6-12; the rotation speed of the centrifugation is 3000-3500r/min, and the centrifugation time is 20-30 min.
Preferably, the pH is adjusted to 4.0-5.0 in step (3); (NH)4)2SO4The saturation of the catalyst reaches 50-65%; the temperature of the refrigerator is 3 ℃; the rotation speed of the centrifugation is 3500-4000r/min, and the centrifugation time is 15-20 min.
Preferably, the concentration of NaOH in the step (4) is 0.18 mol/L.
Preferably, the buffer in the step (5) is Tris-HCl buffer, and the pH value of the buffer is 7.5; the temperature of the refrigerator is 5 ℃; the temperature of vacuum freeze drying is-35 to-40 ℃.
Compared with the prior art, the invention has the following beneficial effects:
processing purple radix puerariae into purple radix puerariae powder, adding NaOH, magnetically stirring, centrifuging and taking supernate; then adjusting the pH of the supernatant and adding (NH)4)2SO4Leaching and collecting the precipitate; dissolving the precipitate with NaOH, filling into dialysis bag, dialyzing with buffer solution, and vacuum freeze drying. The whole extraction process is simple to operate, and is designed for the purple radix puerariae, so that the extraction rate of selenium-containing antioxidant active ingredients in the purple radix puerariae can be improved. Therefore, the eating amount of the purple radix puerariae can be guided, and the utilization value of the purple radix puerariae is improved.
Detailed Description
The technical solutions of the present invention are described in detail below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples the dialysis bag has a flattened width of 25mm and a molecular weight cut-off of 8 kD.
Example 1
A method for extracting selenium-containing antioxidant active ingredients from purple radix Puerariae comprises the following steps:
(1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder; the purple radix Puerariae powder can completely pass through 80 mesh sieve; weighing the mass of the purple radix puerariae powder;
(2) adding 0.15mol/L NaOH into purple radix Puerariae powder, magnetically stirring at room temperature, centrifuging at 3500r/min for 30min, and collecting supernatant;
(3) adding HCl to the supernatant to adjust pH to 5.0, and adding(NH4)2SO4Keeping the saturation degree to 65%, standing in a refrigerator at 3 deg.C for 10h, centrifuging at 4000r/min for 20min, and collecting precipitate;
(4) adding NaOH solution with concentration of 0.18mol/L into the precipitate for dissolving, and transferring into a dialysis bag;
(5) and (3) placing the dialysis bag into a Tris-HCl buffer solution with the pH value of 7.5, placing the dialysis bag into a refrigerator with the temperature of 5 ℃ for dialysis for 20 hours, finally carrying out vacuum freeze drying, and weighing the dried mass, namely the mass of the extracted selenoprotein.
In this embodiment, the feed-liquid ratio of the purple radix puerariae powder to NaOH in step (2) is 1: 12; the temperature of the vacuum freeze-drying in the step (5) is-40 ℃.
Example 2
A method for extracting selenium-containing antioxidant active ingredients from purple radix Puerariae comprises the following steps:
(1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder; the purple radix Puerariae powder can completely pass through 70 mesh sieve; weighing the mass of the purple radix puerariae powder;
(2) adding 0.15mol/L NaOH into purple radix Puerariae powder, magnetically stirring at room temperature, centrifuging at 3250r/min for 25min, and collecting supernatant;
(3) adding HCl to the supernatant to adjust the pH to 4.5, and then adding (NH)4)2SO4Keeping the saturation at 57.5%, standing in a refrigerator at 3 deg.C for 9h, centrifuging at 3750r/min for 17.5min, and collecting precipitate;
(4) adding NaOH solution with concentration of 0.18mol/L into the precipitate for dissolving, and transferring into a dialysis bag;
(5) and (3) placing the dialysis bag into a Tris-HCl buffer solution with the pH value of 7.5, placing the dialysis bag into a refrigerator with the temperature of 5 ℃ for dialysis for 19 hours, finally carrying out vacuum freeze drying, and weighing the dried mass, namely the mass of the extracted selenoprotein.
In this embodiment, the feed-liquid ratio of the purple radix puerariae powder to NaOH in step (2) is 1: 9; the temperature of the vacuum freeze-drying in the step (5) was-37.5 ℃.
Example 3
A method for extracting selenium-containing antioxidant active ingredients from purple radix Puerariae comprises the following steps:
(1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder; the purple radix Puerariae powder can completely pass through 60 mesh sieve; weighing the mass of the purple radix puerariae powder;
(2) adding 0.15mol/L NaOH into purple radix Puerariae powder, magnetically stirring at room temperature, centrifuging at 3000r/min for 20min, and collecting supernatant;
(3) adding HCl to the supernatant to adjust the pH to 4.0, and then adding (NH)4)2SO4Keeping the saturation degree to 50%, standing in a refrigerator at 3 deg.C for 8h, centrifuging at 3500r/min for 15min, and collecting precipitate;
(4) adding NaOH solution with concentration of 0.18mol/L into the precipitate for dissolving, and transferring into a dialysis bag;
(5) and (3) placing the dialysis bag into Tris-HCl buffer solution with the pH value of 7.5, placing the dialysis bag into a refrigerator with the temperature of 5 ℃ for dialysis for 18 hours, and finally carrying out vacuum freeze drying to obtain the selenium protein extract, wherein the dried weight is weighed, namely the weight of the extracted selenium protein.
In this embodiment, the feed-liquid ratio of the purple radix puerariae powder to NaOH in step (2) is 1: 6; the temperature of the vacuum freeze-drying in the step (5) is-35 ℃.
Comparative example 1
The extraction method is disclosed in application No. CN201910700446.8, and the disclosed optimal extraction process parameters are adopted.
Determination of extraction Rate
The extraction rates of selenoprotein and selenium in examples 1-3 were determined by hydride yard fluorescence spectrometry according to the method of GB/T5009.93-2010, and the results are shown in Table 1 below.
Selenoprotein extraction rate (%) (mass of extracted selenoprotein ÷ mass of purple pueraria thomsonii powder) × 100;
selenium extraction rate (%) × 100 (selenium content in extracted selenoprotein ÷ selenium content in purple radix puerariae powder);
TABLE 1 Selenoprotein extraction and selenium extraction of the invention
| Group of | Extraction ratio of selenoprotein (%) | Selenium extraction (%) |
| Example 1 | 3.93 | 37.39 |
| Example 2 | 4.36 | 42.17 |
| Example 3 | 4.07 | 39.98 |
| Comparative example 1 | 2.24 | 21.25 |
As can be seen from Table 1, when the extraction method of the invention is used for extracting selenoprotein from purple radix Puerariae, the extraction rate of selenoprotein reaches 4.36% at most, and the extraction rate of selenoprotein also reaches 42.17%, which is obviously higher than that of the comparative example.
Therefore, the extraction method can improve the extraction rate of the selenoprotein in the purple radix puerariae, guides the eating amount of the purple radix puerariae, and further improves the utilization value of the purple radix puerariae.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (6)
1. A method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae is characterized by comprising the following steps:
(1) removing impurities from radix Puerariae of purple radix Puerariae, slicing, oven drying, and pulverizing to obtain purple radix Puerariae powder;
(2) adding NaOH into the purple radix puerariae powder, magnetically stirring at room temperature, centrifuging, and taking supernatant;
(3) adding HCl to the supernatant to adjust pH, and then adding (NH)4)2SO4Standing in a refrigerator for 8-10h, centrifuging, and collecting precipitate;
(4) adding NaOH solution into the precipitate to dissolve, and transferring into dialysis bag;
(5) and (3) putting the dialysis bag into the buffer solution, putting the dialysis bag into a refrigerator for dialysis for 18-20h, and finally performing vacuum freeze drying.
2. The method of claim 1, wherein the purple radix Puerariae powder in step (1) is passed through a 60-80 mesh sieve.
3. The method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae as claimed in claim 1, wherein the concentration of NaOH in step (2) is 0.15mol/L, and the feed-liquid ratio of purple radix puerariae powder to NaOH is 1: 6-12; the rotation speed of the centrifugation is 3000-3500r/min, and the centrifugation time is 20-30 min.
4. The purple-colored liquid of claim 1The method for extracting the selenium-containing antioxidant active ingredient from the pachyrhizua angulatus is characterized in that the pH value in the step (3) is adjusted to 4.0-5.0; (NH)4)2SO4The saturation of the catalyst reaches 50-65%; the temperature of the refrigerator is 3 ℃; the rotation speed of the centrifugation is 3500-4000r/min, and the centrifugation time is 15-20 min.
5. The method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae as claimed in claim 1, wherein the concentration of NaOH in step (4) is 0.18 mol/L.
6. The method for extracting selenium-containing antioxidant active ingredients from Pueraria thomsonii according to claim 1, wherein the buffer in step (5) is Tris-HCl buffer, and the pH value of the buffer is 7.5; the temperature of the refrigerator is 5 ℃; the temperature of vacuum freeze drying is-35 to-40 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010616751.1A CN111700149A (en) | 2020-06-29 | 2020-06-29 | Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010616751.1A CN111700149A (en) | 2020-06-29 | 2020-06-29 | Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111700149A true CN111700149A (en) | 2020-09-25 |
Family
ID=72544019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010616751.1A Pending CN111700149A (en) | 2020-06-29 | 2020-06-29 | Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111700149A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760344A (en) * | 2021-01-28 | 2021-05-07 | 北部湾大学 | Enzymolysis method for extracting selenomethionine from oysters |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101628935A (en) * | 2009-07-21 | 2010-01-20 | 余芳 | Method for extracting selenium protein from selenium-enriched green tea |
| CN102775466A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Preparation method of selenium-containing protein in selenium-enriched yeast |
| CN102928500A (en) * | 2012-11-15 | 2013-02-13 | 中华人民共和国浙江出入境检验检疫局 | Method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS (Inductively Coupled Plasma Mass Spectrometry) way |
| CN104365988A (en) * | 2014-11-03 | 2015-02-25 | 苏州硒谷科技有限公司 | Method for extracting selenium-containing protein by using high-selenium edible fungus |
| CN108546280A (en) * | 2018-03-21 | 2018-09-18 | 中国地质大学（武汉） | A kind of extracting method of the vegetable protein containing selenium |
| CN109007688A (en) * | 2018-06-15 | 2018-12-18 | 恩施硒谷科技股份有限公司 | A kind of preparation method of selenium-rich pueraria lobata activity powder |
-
2020
- 2020-06-29 CN CN202010616751.1A patent/CN111700149A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101628935A (en) * | 2009-07-21 | 2010-01-20 | 余芳 | Method for extracting selenium protein from selenium-enriched green tea |
| CN102775466A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Preparation method of selenium-containing protein in selenium-enriched yeast |
| CN102928500A (en) * | 2012-11-15 | 2013-02-13 | 中华人民共和国浙江出入境检验检疫局 | Method for detecting organic selenium, protein selenium or polysaccharide selenium in marine products through microwave digestion-ICP-MS (Inductively Coupled Plasma Mass Spectrometry) way |
| CN104365988A (en) * | 2014-11-03 | 2015-02-25 | 苏州硒谷科技有限公司 | Method for extracting selenium-containing protein by using high-selenium edible fungus |
| CN108546280A (en) * | 2018-03-21 | 2018-09-18 | 中国地质大学（武汉） | A kind of extracting method of the vegetable protein containing selenium |
| CN109007688A (en) * | 2018-06-15 | 2018-12-18 | 恩施硒谷科技股份有限公司 | A kind of preparation method of selenium-rich pueraria lobata activity powder |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760344A (en) * | 2021-01-28 | 2021-05-07 | 北部湾大学 | Enzymolysis method for extracting selenomethionine from oysters |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102250731A (en) | Raw materials of health preserving white spirit, dendrobium health preserving white spirit and manufacturing method thereof | |
| CN102987105A (en) | Duck feed | |
| CN107095229A (en) | A kind of cordyceps sinensis silkie soup and preparation method thereof | |
| CN112535239B (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
| CN111700149A (en) | Method for extracting selenium-containing antioxidant active ingredients from purple radix puerariae | |
| CN107549564A (en) | A kind of preparation method of russule beverage | |
| CN113197258A (en) | Selenium-rich vine tea and preparation method thereof | |
| CN108782666B (en) | Vine tea cake and preparation method thereof | |
| CN1957731A (en) | Fast dissolving fruit powder, and preparation method | |
| CN106720801B (en) | Burdock tea rich in inulin | |
| CN113201078B (en) | Preparation method and application of rice bran polysaccharide zinc chelate | |
| CN114052108A (en) | Formula and preparation method of lucid ganoderma and jasmine tea beverage | |
| CN103520470A (en) | Preparation method of Chinese medicine mixture | |
| CN113383840A (en) | Selenium-rich vine tea | |
| CN111544460A (en) | Method for extracting selenium-containing antioxidant active ingredients from selenium-rich moringa oleifera tea | |
| CN112931862A (en) | Rock candy cubilose and preparation method thereof | |
| CN110973435A (en) | Licorice root and platycodon root particle solid beverage | |
| CN111154600A (en) | Preparation method of semi-sweet cool yellow wine | |
| CN107568296A (en) | A kind of angelica keiskei koidz vegetable biscuit and its preparation method and application | |
| CN110754592A (en) | Rhizoma amorphophalli coarse cereal beverage and preparation method and application thereof | |
| CN112244261B (en) | Composite edible salt and preparation method thereof | |
| KR102468019B1 (en) | Plant mixed concentrate mainly containing Korean Dendropanax and foods using the concentrate | |
| CN109170401B (en) | Preparation method of sow thistle beverage | |
| CN111713594A (en) | Method for extracting selenium-containing antioxidant component from purple Chinese yam by alkaline method | |
| CN114711420A (en) | Composition of health-care product capsule and its production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200925 |