CN111691220A - In-vitro life detection material and preparation method thereof - Google Patents
In-vitro life detection material and preparation method thereof Download PDFInfo
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- CN111691220A CN111691220A CN202010577524.2A CN202010577524A CN111691220A CN 111691220 A CN111691220 A CN 111691220A CN 202010577524 A CN202010577524 A CN 202010577524A CN 111691220 A CN111691220 A CN 111691220A
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- detection material
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- vitro life
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H11/00—Pulp or paper, comprising cellulose or lignocellulose fibres of natural origin only
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
-
- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
- D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Food Science & Technology (AREA)
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- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention relates to the technical field of detection materials, and discloses an in vitro life detection material, which consists of 100 percent of linter cotton pulp with the particle size not greater than 100nm, wherein the in vitro life detection material contains 1.5-2.0 mg/kg of aluminum element, 0.2-0.5 mg/kg of barium element, 100-130 mg/kg of calcium element and 15.0-20.0 mg/kg of sodium element, and the preparation method comprises the following steps: pulping 100% of cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is not less than twenty minutes; pulping the pulped pulp by a pulping machine for at least thirty minutes, wherein the beating degree of the finished pulp is not less than 36 ℃; rinsing the ground pulp by using 100% of underground water for not less than twenty minutes; the rinsed pulp is molded and dried on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
Description
Technical Field
The invention relates to the technical field of detection materials, in particular to an in vitro life detection material and a preparation method thereof.
Background
In vitro diagnosis refers to products and services for obtaining clinical diagnosis information by detecting samples (blood, body fluid, tissue, etc.) of a human body outside the human body, and subdivided fields mainly include blood and body fluid diagnosis, biochemical diagnosis, immunodiagnosis, microbiological diagnosis, molecular diagnosis, and point of care Products (POCT), and the like, wherein the point of care Products (POCT) are the most commonly used and widely used fields, and the point of care Products (POCT) refer to clinical tests and bedside tests performed beside a patient, and are not always performed by clinical testers. The POCT analysis method is a novel method for immediately analyzing a sample on a sampling site, a complex processing procedure of the sample in laboratory inspection is omitted, and an inspection result is quickly obtained, some problems commonly exist in the currently used POCT, such as the reaction is not sensitive enough, and the color change is not obvious or too slow after the sample is contacted with the POCT, so that the effect and the action of instant diagnosis cannot be achieved; the color change span after the sample contacts the POCT is too small, and the difficulty of judging and distinguishing the pathology is improved. For example, chinese patent application publication No.: CN106333695A, application published 2017, No. 01/18, entitled POCT detection device, firstly, the finger of a patient is placed on a second base of a hematocrit measurement adapter, fixing the finger through a second cover plate, irradiating the finger by a second light source, measuring oxidized hemoglobin and unoxidized hemoglobin in the capillary vessel of the fingertip of the patient so as to obtain the total amount of hemoglobin, further obtaining hematocrit through a formula, that is, the hematocrit of the patient is obtained by the hematocrit test adapter, then the blood of the patient is dropped on the test paper of the test strip, the test strip is placed on the first base, the test piece is fixed through the first cover plate, the number of the objects to be detected is obtained through irradiation of the first light source, the hematocrit obtained through the hematocrit determination adapter is brought into a detection formula, and a test result of the objects to be detected is obtained. The POCT detection device has the defects that the reaction is not sensitive enough, the color change is not obvious or is slow after the sample is contacted with the POCT, the effect and the function of instant diagnosis cannot be achieved, the color change span is too small after the sample is contacted with the POCT, and the judgment and the differentiation of pathology are difficult.
Disclosure of Invention
The invention provides an in vitro life detection material with sensitive reaction and obvious color change and a preparation method thereof, aiming at solving the defects that the POCT detection device in the prior art has not sensitive reaction, the color change is not obvious or too slow to achieve the effect and action of instant diagnosis after a sample is contacted with the POCT, the color change span is too small after the sample is contacted with the POCT, and the judgment and the differentiation of pathology are difficult.
In order to achieve the purpose, the invention adopts the following technical scheme:
an in vitro life detection material consists of 100% of linter cotton pulp, wherein the grain size of the linter cotton pulp is not more than 100nm, and the in vitro life detection material contains 1.5-2.0 mg/kg of aluminum element, 0.2-0.5 mg/kg of barium element, 100-130 mg/kg of calcium element and 15.0-20.0 mg/kg of sodium element.
The in vitro life detection material prepared by the following preparation method by taking the raw materials in the component ratio has the main performance indexes that the filtration rate is less than or equal to 100S, the longitudinal water absorption height is more than or equal to 105mm, the transverse water absorption height is more than or equal to 95mm, the dry burst is more than or equal to 330Kpa, the wet burst is more than or equal to 350Kpa, and the water absorption capacity is more than or equal to 345 g/square meter. In the component proportion, 100 percent of the linter cotton pulp is used as a basic component for detecting the in vitro life, the particle size is not more than 100nm, so that the detection material has higher water absorption, bursting strength and higher filtering speed, and the limitation of microelements such as aluminum, barium, calcium, sodium and the like which play a reaction mechanism is an important determinant factor for ensuring that the in vitro life detection material has high sensitivity and high detection effect.
Preferably, the in vitro life detection material also contains <8.0mg/kg of iron element.
Preferably, the in vitro life detection material further contains <8.0mg/kg of magnesium element.
Preferably, the in vitro life detection material also contains <8.0mg/kg of copper element.
Preferably, the in vitro life detection material also contains chlorine element of less than 100 mg/kg.
Preferably, the in vitro life detection material has a quantitative value of 185 +/-5 g/square meter. And determining proper quantitative values to balance the balance relationship among the water absorption, the burst strength and the filtration rate, wherein if the quantitative values are too high, the filtration rate is too low, and if the quantitative values are too low, the water absorption and the burst strength are too low, so that the use is influenced.
A method for preparing in vitro life detection material comprises the following steps:
a) pulping 100% of cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is not less than twenty minutes;
b) pulping the pulped pulp by a pulping machine for at least thirty minutes, wherein the beating degree of the finished pulp is not less than 36 ℃;
c) rinsing the ground pulp by using 100% of underground water for not less than twenty minutes;
d) and (3) making, molding and drying the rinsed pulp on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
The in vitro life detection material and the preparation method thereof have the following advantages: high filtration rate, high water absorption, high bursting strength, high sensitivity, high accuracy of test result, and convenient and safe use.
Detailed Description
The technical solution of the present invention is further specifically described below by way of examples and with reference to table 1.
Comparative example
The test paper comprises a sample pad, an inflammation marker antibody magnetic rare earth fluorescent microsphere label pad, a coating film and a water absorption pad, wherein three inflammation marker quantitative detection lines and a quality control region C line are arranged on the coating film, and after the detected substance in a sample is concentrated by a magnetic field, the detected substance is detected by a low-background and high-sensitivity fluorescence immunochromatography method. The main performance indexes comprise that the filtration rate is less than or equal to 130S, the longitudinal water absorption height is more than or equal to 80mm, the transverse water absorption height is more than or equal to 76mm, the dry burst is more than or equal to 273Kpa, the wet burst is more than or equal to 298Kpa, and the water absorption capacity is more than or equal to 311 g/square meter.
Example 1
Preparing in-vitro life detection materials by taking 100% of linter cotton pulp with the particle size not greater than 100nm according to the following steps:
a) pulping the cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is twenty minutes;
b) pulping the pulped pulp through a pulping machine for thirty minutes, wherein the beating degree of the finished pulp is 36 ℃;
c) rinsing the ground pulp by using 100% of underground water for twenty minutes;
d) and (3) making, molding and drying the rinsed pulp on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
Example 2
Preparing in-vitro life detection materials by taking 100% of linter cotton pulp with the particle size not greater than 100nm according to the following steps:
a) pulping the cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is thirty-five minutes;
b) pulping the pulped pulp by a pulping machine for forty-five minutes, wherein the beating degree of the finished pulp is 39 ℃;
c) rinsing the ground pulp by using 100% of underground water for thirty-five minutes;
d) and (3) making, molding and drying the rinsed pulp on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
Example 3
Preparing in-vitro life detection materials by taking 100% of linter cotton pulp with the particle size not greater than 100nm according to the following steps:
a) pulping the cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is thirty-five minutes;
b) pulping the pulped pulp by a pulping machine for forty-five minutes, wherein the beating degree of the finished pulp is 39 ℃;
c) rinsing the ground pulp by using 100% of underground water for thirty-five minutes;
d) and (3) making, molding and drying the rinsed pulp on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
The in vitro life test materials prepared in the comparative example and examples 1 to 3 were subjected to performance tests, and the test results are shown in table 1.
TABLE 1
| Item number | Comparative example | Example 1 | Example 2 | Example 3 |
| Filtration rate S | 108 | 86 | 91 | 98 |
| Longitudinal water absorption height mm | 93 | 105 | 111 | 118 |
| Transverse water absorption height mm | 84 | 95 | 98 | 105 |
| Dry burst Kpa | 258 | 273 | 281 | 294 |
| Wet bursting strength Kpa | 284 | 298 | 309 | 315 |
| Water absorption capacity g/square meter | 273 | 311 | 319 | 325 |
| Quantitative g/square meter | 163 | 182 | 189 | 192 |
| Aluminum mg/kg | 0.4 | 1.6 | 1.8 | 2.0 |
| Barium mg/kg | 0 | 0.25 | 0.34 | 0.48 |
| Calcium mg/kg | 71.5 | 105.6 | 118.4 | 128.6 |
| Sodium mg/kg | 6.8 | 15.4 | 17.8 | 19.4 |
| Iron mg/kg | 0 | 8.3 | 9.8 | 9.9 |
| Magnesium mg/kg | 0 | 8.1 | 9.4 | 10.2 |
| Copper mg/kg | 0 | 8.6 | 10.4 | 11.8 |
| Chlorine mg/kg | 74.8 | 105.2 | 113.6 | 124.8 |
According to the contents in table 1, the in vitro life detection material obtained by the invention has the characteristics of high filtration efficiency, high water absorption and high bursting strength, and has a plurality of microelements such as aluminum, barium, calcium, sodium and the like which determine reaction mechanisms, so that the in vitro life detection material obtained by the invention has high detection sensitivity and accuracy.
The raw materials and equipment used in the present invention are all the ones that are commonly used in the art unless otherwise specified, and the methods used in the present invention are all the ones that are conventional in the art unless otherwise specified.
The in vitro life detection material and the preparation method thereof have the beneficial effects of high filtration rate, high water absorption, high bursting strength, high sensitivity, high test result accuracy and convenience and safety in use.
Claims (8)
1. An in vitro life detection material is characterized by consisting of 100% of linter cotton pulp, wherein the particle size of the linter cotton pulp is not more than 100nm, and the in vitro life detection material contains 1.5-2.0 mg/kg of aluminum element, 0.2-0.5 mg/kg of barium element, 100-130 mg/kg of calcium element and 15.0-20.0 mg/kg of sodium element.
2. The in vitro life test material of claim 1, wherein the in vitro life test material further comprises <8.0mg/kg of iron element.
3. The in vitro life detection material according to claim 1 or 2, wherein the in vitro life detection material further comprises <8.0mg/kg of magnesium.
4. The in vitro life detection material according to claim 1 or 2, wherein the in vitro life detection material further comprises <8.0mg/kg of copper.
5. The in vitro life detection material according to claim 1 or 2, wherein the in vitro life detection material further comprises <8.0mg/kg of copper.
6. The in vitro life detection material according to claim 1 or 2, wherein the in vitro life detection material further comprises <100mg/kg of chlorine.
7. The in vitro life detection material according to claim 1 or 2, wherein the quantitative determination of the in vitro life detection material is 185 ± 5g per square meter.
8. A method for preparing the in vitro life detection material according to claims 1 to 7, comprising the steps of:
a) pulping 100% of cotton linter pulp by a pulper, wherein 100% of underground water is used for pulping, and the pulping time is not less than twenty minutes;
b) pulping the pulped pulp by a pulping machine for at least thirty minutes, wherein the beating degree of the finished pulp is not less than 36 ℃;
c) rinsing the ground pulp by using 100% of underground water for not less than twenty minutes;
d) and (3) making, molding and drying the rinsed pulp on an inclined wire paper machine or a flow measuring type round wire paper machine to obtain the target in-vitro life detection material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010577524.2A CN111691220A (en) | 2020-06-23 | 2020-06-23 | In-vitro life detection material and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010577524.2A CN111691220A (en) | 2020-06-23 | 2020-06-23 | In-vitro life detection material and preparation method thereof |
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