CN111690684B - 一种组合物及其应用 - Google Patents
一种组合物及其应用 Download PDFInfo
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- CN111690684B CN111690684B CN202010443531.3A CN202010443531A CN111690684B CN 111690684 B CN111690684 B CN 111690684B CN 202010443531 A CN202010443531 A CN 202010443531A CN 111690684 B CN111690684 B CN 111690684B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
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- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
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- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8775—Murine embryos
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/12—Animals modified by administration of exogenous cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
Description
培养基 | KSOM(200mL) | M2(200mL) |
EDTA(disodium 0.01mM) | 0.00076g | / |
NaCl | 1.119g | 1.1068g |
KCl | 0.037g | 0.070g |
CaCl<sub>2</sub>·2H<sub>2</sub>O | 1.71mM | 1.71mM |
KH<sub>2</sub>PO<sub>4</sub> | 0.0095g | 0.032g |
MgSO<sub>4</sub> | 0.00482g | 0.0283g |
NaHCO<sub>3</sub> | 0.420g | 0.070g |
Na lactate | 0.280mL | 0.62mL |
Na pyruvate | 0.0044g | 0.0073g |
Glucose | 0.0072g | 0.1g |
Pen/Strep/Glu | 1mL | 2mL |
HEPES | / | 0.994g |
EDTA(100mM) | / | 200μL |
Gentamycin | / | 200μL |
Phenol red(0.5%) | 20μL | 100μL |
BSA | 0.2g | 0.8g |
水 | 加水至200mL | 加水至200mL |
Claims (43)
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CN2020103662381 | 2020-04-30 | ||
CN202010366238 | 2020-04-30 |
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Publication Number | Publication Date |
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CN111690684A CN111690684A (zh) | 2020-09-22 |
CN111690684B true CN111690684B (zh) | 2022-07-29 |
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CN202010443531.3A Active CN111690684B (zh) | 2020-04-30 | 2020-05-22 | 一种组合物及其应用 |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2021174742A1 (zh) * | 2020-03-06 | 2021-09-10 | 生物岛实验室 | 一种动物的制备方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1814750A (zh) * | 2005-02-04 | 2006-08-09 | 上海交通大学附属儿童医院 | 一种哺乳动物体细胞核移植方法 |
CN101182489A (zh) * | 2007-11-19 | 2008-05-21 | 戴蕴平 | 一种动物体细胞克隆方法 |
CN102229964A (zh) * | 2011-05-23 | 2011-11-02 | 内蒙古农业大学 | 一种高效制备绵羊克隆胚胎的方法 |
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