CN111690392A - Environment-friendly microbial composite paraffin removal and prevention agent for oil well - Google Patents
Environment-friendly microbial composite paraffin removal and prevention agent for oil well Download PDFInfo
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- CN111690392A CN111690392A CN202010677879.9A CN202010677879A CN111690392A CN 111690392 A CN111690392 A CN 111690392A CN 202010677879 A CN202010677879 A CN 202010677879A CN 111690392 A CN111690392 A CN 111690392A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
- C09K8/524—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning organic depositions, e.g. paraffins or asphaltenes
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
- C09K8/528—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning inorganic depositions, e.g. sulfates or carbonates
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/54—Compositions for in situ inhibition of corrosion in boreholes or wells
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
- C23F11/12—Oxygen-containing compounds
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
- C23F11/14—Nitrogen-containing compounds
- C23F11/145—Amides; N-substituted amides
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- C—CHEMISTRY; METALLURGY
- C23—COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
- C23F—NON-MECHANICAL REMOVAL OF METALLIC MATERIAL FROM SURFACE; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL; MULTI-STEP PROCESSES FOR SURFACE TREATMENT OF METALLIC MATERIAL INVOLVING AT LEAST ONE PROCESS PROVIDED FOR IN CLASS C23 AND AT LEAST ONE PROCESS COVERED BY SUBCLASS C21D OR C22F OR CLASS C25
- C23F11/00—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent
- C23F11/08—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids
- C23F11/10—Inhibiting corrosion of metallic material by applying inhibitors to the surface in danger of corrosion or adding them to the corrosive agent in other liquids using organic inhibitors
- C23F11/173—Macromolecular compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/20—Controlling water pollution; Waste water treatment
- Y02A20/204—Keeping clear the surface of open water from oil spills
Abstract
The invention provides an oil well environment-friendly microbial composite paraffin remover and inhibitor, which comprises 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent; the wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus fermentation liquid; the components of the strain nutrient solution comprise 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water; the first surfactant is a fluorocarbon surfactant FC-3B or oleamide betaine FDCH-1; the second surfactant is polyoxyethylene octyl phenol ether-10 or SP 80; the mutual solvent is n-octanol or ethylene glycol monobutyl ether. The composite wax removing and preventing agent effectively overcomes the technical defects of the existing chemical method and microbial method for removing and preventing wax, and has the advantages of economy, environmental protection, high wax dissolving speed in thick oil, strong anti-scouring capability, long acting period and the like.
Description
Technical Field
The invention belongs to the technical field of oil field gathering and transportation, relates to the technical field of oil field gathering and transportation wax removal and prevention chemicals, and particularly relates to an environment-friendly microbial composite wax removal and prevention agent for an oil well. The invention solves the defects of high toxicity, non-ideal effect and the like of the existing paraffin removal and prevention agent through biological and chemical methods.
Background
Crude oil contains various amounts of paraffins, which are generally in solution under reservoir conditions. When crude oil rises from the bottom of a well and flows through an oil pipe, liquid paraffin in the crude oil is separated out along with the reduction of the temperature and the pressure of the pipeline and is adsorbed on the pipe wall of the oil pipe to cause wax deposition of the oil well, the harmfulness is high, the oil well can be directly blocked seriously, and the yield of the oil field is suddenly reduced or stopped. At present, the wax content of most of domestic oil fields is over 20 percent, for example, oil wells in the safety operation area of Changqing oil fields have the wax content of 25.46 percent, and the normal development and transportation of crude oil are influenced by the problems of wax precipitation and corrosion of the oil wells.
The most common paraffin removal method is to remove and prevent paraffin by a chemical method and dissolve paraffin by organic solvents such as benzene, xylene and the like, but the paraffin removal and prevention agents are flammable and explosive and have great harm to the environment and human bodies. The existing microbial paraffin remover and inhibitor in the market has the defects of weak anti-scouring capability, short action period, slow wax dissolving rate and the like.
Disclosure of Invention
Aiming at the technical defects of adopting a chemical method and a microbiological method to clear and prevent wax in the prior art, in particular to the use of a non-environment-friendly paraffin solvent, the invention provides an oil well environment-friendly microbial composite wax clearing and preventing agent. The invention achieves the purpose of removing and preventing wax by mixing the synergistic action of the wax-philic microorganism and the surfactant, and utilizing the metabolism of the microorganism in the stratum and the wetting and permeating action of the surfactant to quickly dissolve the solid paraffin on the pipe wall and the well rod and degrade the long-chain alkane in the crude oil. The composite wax removing and preventing agent is economical and environment-friendly, has high wax dissolving speed in thick oil, strong anti-scouring capability and long action period, and is an efficient and environment-friendly composite wax removing and preventing agent.
In order to achieve the above object, the present invention adopts the following technical solutions.
The environment-friendly microbial composite paraffin remover and inhibitor for the oil well comprises, by mass, 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent.
The wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2: 1.
The strain nutrient solution comprises, by mass, 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water.
The first surfactant is one of fluorocarbon surfactant FC-3B and oleamide betaine FDCH-1.
The second surfactant is one of polyoxyethylene octyl phenol ether-10 and SP 80.
The mutual solvent is one of n-octanol and ethylene glycol monobutyl ether.
The paraffin dissolution rate of a single wax-philic strain is often limited because the mixed strain is divided into different fractions for processing crude oil components such as wax, colloid and asphaltene in the crude oil. In addition, the oil well operation environment is different from the crude oil stratum environment, and the tolerance and the suitability of a single wax-philic strain to different environments are also different. In order to improve the dissolving rate of the wax-philic strains to paraffin and improve the universality of microbial wax-removing and preventing products, the invention screens and compounds various wax-philic strains for use, and determines the wax-philic mixed bacterial liquid used by combining the wax-philic strains of Pseudomonas, Bacillus and Micrococcus after a plurality of attempts. Specifically, the Pseudomonas aeruginosa is selected from the cerophilic wax strains of the Pseudomonas aeruginosa, and is preserved in China general microbiological culture collection center in 2009 for 1 month and 19 days, wherein the preservation number is CGMCC 1.9054; bacillus subtilis (Bacillus subtilis) is selected from Bacillus cereus strain, and is preserved in China general microbiological culture collection center in 1972 in 3 months and 1 day, with the preservation number of CGMCC 1.821; micrococcus cerophilus is selected from Micrococcus terreus (Micrococcus terreus), and is preserved in China general microbiological culture Collection center in 2007 in 1 month and 12 days, with the preservation number of CGMCC 1.7054. Pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus strains used in the invention are all purchased from China general microbiological culture collection center. The culture fermentation broth is preferably an expanded culture fermentation broth in a logarithmic growth phase, the compound volume ratio of the pseudomonas aeruginosa culture fermentation broth to the pseudomonas subtilis culture broth to the micrococcus terrae culture fermentation broth is 1:2:1, and the culture broth has a better characteristic on the degradation effect of paraffin.
The strain nutrient solution is a specially-prepared mixed liquid which is suitable for coexistence of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus and keeps excellent microbial performance. The first surfactant and the second surfactant selected by the invention are products developed and produced by Shaanxi Fengden petrochemical company Limited, and have good compatibility with wax-philic mixed bacteria liquid and strain nutrient solution. The mutual solvent is one of n-octanol and ethylene glycol monobutyl ether, and is a commercially available product.
Through determination, the amount of the pseudomonas aeruginosa, the bacillus subtilis and the micrococcus terreus in the composite paraffin remover and inhibitor is 5-9 × 108one/mL.
In addition, the invention also provides application of the oil well environment-friendly microbial composite paraffin removal and prevention agent in oil field gathering and transportation operation. The composite wax cleaning and preventing agent has stronger adsorption force and anti-scouring capability, microorganisms adsorbed on the wall of an oil pipe cannot be obviously reduced due to the scouring effect, so that the action period of the wax cleaning and preventing agent is prolonged, and the wax dissolving rate reaches 0.044 g/min-0.049 g/min.
The field application proves that the composite paraffin removal and prevention agent of the invention has SO4 on the stratum2-The ions have certain consumption effect, can reduce substrates required by sulfate reducing bacteria, reduce the activity of the sulfate reducing bacteria, not only can play a role in wax prevention and wax removal, but also can inhibit the growth and reproduction of the sulfate reducing bacteria and play a role in inhibiting corrosion of metal pipelines.
Compared with the prior art, the oil well environment-friendly microbial composite paraffin removal and prevention agent disclosed by the invention at least has the following beneficial effects or advantages.
1) The oil well environment-friendly microbial composite paraffin remover and inhibitor comprises, by mass, 15-20 parts of a paraffin-philic mixed bacterial liquid, 15-60 parts of a strain nutrient solution, 5-9 parts of a first surfactant, 5-9 parts of a second surfactant and 2-4 parts of a mutual solvent. Through multiple test trials of the inventor of the invention, the paraffin-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2:1, and has an excellent degradation effect on paraffin in crude oil.
2) The oil well environment-friendly microbial composite paraffin removal and prevention agent disclosed by the invention is environment-friendly, and has a high paraffin dissolution rate, and the bacterial concentration reaches 5-9 × 108The wax cleaning agent has the advantages that the wax cleaning agent has a high adsorption capacity and a high anti-scouring capacity, microorganisms adsorbed on the wall of an oil pipe cannot be obviously reduced due to the scouring effect, the action period of the wax cleaning agent is prolonged, and the wax dissolving rate reaches 0.044-0.049 g/min.
3) The oil well environment-friendly microbial composite paraffin removal and prevention agent can consume SO4 in stratum2-The ions not only can play a role in wax prevention and wax removal, but also play a role in corrosion inhibition on the metal pipeline.
Detailed Description
In order to facilitate understanding of the objects, technical solutions and effects of the present invention, the present invention will be further described in detail with reference to examples.
Example 1
This example determines the growth of selected Pseudomonas aeruginosa, Bacillus subtilis, and Micrococcus terreus in the crude oil.
Taking 3 150mL conical flasks with the serial numbers of 1#, 2#, and 3#, sterilizing, respectively adding 3g of crude oil sample in Changqing oil field Jingan operation area and 60mL of strain nutrient solution, sequentially adding 1mL of pseudomonas aeruginosa fermentation liquor, 2mL of bacillus subtilis fermentation liquor and 1mL of micrococcus terreus fermentation liquor, placing at 30 ℃, culturing at constant temperature for 5 days under aerobic condition, and counting the growth conditions of each strain in the strain suspension by a hemocytometer method. The results are shown in Table 1.
TABLE 1 growth of Pseudomonas aeruginosa, Bacillus subtilis and Micrococcus terreus in high wax crude oil
| Numbering | Name of microorganism | Inoculation 0h number of bacteria (one/mL) | Inoculation 5 days bacteria count (one/mL) |
| 1# | Pseudomonas aeruginosa | 1.3×106 | 2.1×108 |
| 2# | Bacillus subtilis | 2.4×106 | 4.1×108 |
| 3# | Micrococcus terreus | 1.5×106 | 2.3×108 |
As can be seen from Table 1, the number of strains was 106Increase to 10/mL8The strain/mL indicates that pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus grow well in crude oil of an oil well in a Jingan working area in Changqing oil fields.
Example 2
The embodiment provides a preferable oil well environment-friendly microbial composite paraffin remover and inhibitor.
Respectively taking culture fermentation liquids of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus, and mixing the culture fermentation liquids according to the volume ratio of 1:2:1 to obtain the wax-philic mixed bacterial liquid.
Weighing 90kg of deionized water, 20kg of sweet potato powder, 1kg of liquid paraffin, 1kg of vinasse, 1kg of agar, 1kg of NaCl and 1kg of NH4Cl、2kgKH2PO4、1kgCaCl2、1kgNa2SO4Mixing, and making into strain nutrient solution.
And compounding 15kg of the wax-philic mixed bacterial liquid, 15kg of strain nutrient solution, 5kg of oleamide betaine FDCH-1, 5kg of polyoxyethylene octyl phenol ether-10 and 2kg of ethylene glycol monobutyl ether to obtain the oil well environment-friendly microbial composite paraffin removal and prevention agent.
And performing an effect evaluation test on the oil well environment-friendly microbial composite paraffin remover and inhibitor, namely taking 5g of solid paraffin in an X oil well of the Changqing oil field, soaking the solid paraffin in 15mL of the composite paraffin remover and inhibitor, keeping the temperature of a water bath at 45 +/-1 ℃, observing and recording the time for the solid paraffin to be completely dissolved, and calculating the paraffin dissolving rate of the composite paraffin remover and inhibitor to be 0.044 g/min.
100g of solid paraffin in an oil well in a Jingan working area of the Changqing oil field is taken, stirred and slowly added into 50mL of the composite paraffin remover and inhibitor, and the mixture is subjected to constant temperature water bath at 45 +/-1 ℃ until the mixture is insoluble within 2 hours after paraffin is added. The maximum dissolved paraffin mass of 50mL of the composite paraffin remover/inhibitor is recorded, and the saturated dissolved paraffin amount is calculated to be 0.52 g/mL.
Example 3
On the basis of the embodiment 2, the embodiment provides a preferable oil well environment-friendly microbial composite paraffin removal and prevention agent.
The strain nutrient solution comprises the following components in percentage by weight: 100kg of deionized water, 35kg of sweet potato powder, 5kg of liquid paraffin, 4kg of vinasse, 3kg of agar, 2kg of NaCl and 2kg of NH4Cl、7kgKH2PO4、2kgCaCl2、2kgNa2SO4。
And (3) compounding 20kg of the wax-philic mixed bacterial liquid, 60kg of strain nutrient solution, 9kg of fluorocarbon surfactant FC-3B, 9kg of SP80 and 4kg of ethylene glycol monobutyl ether to obtain the environment-friendly microbial composite wax remover and inhibitor for the oil well.
The oil well environment-friendly microbial composite paraffin removal and prevention agent is subjected to an effect evaluation test, 5g of solid paraffin in an X oil well of a Changqing oil field is taken and soaked in 15mL of the composite paraffin removal and prevention agent, the water bath is kept at the constant temperature of 45 +/-1 ℃, the time for the solid paraffin to be completely dissolved is observed and recorded, and the paraffin dissolving rate of the composite paraffin removal and prevention agent is calculated to be 0.049 g/min.
100g of solid paraffin in an oil well in a Jingan working area of the Changqing oil field is taken, stirred and slowly added into 50mL of the composite paraffin remover and inhibitor, and the mixture is subjected to constant temperature water bath at 55 +/-1 ℃ until the mixture is insoluble within 2 hours after paraffin is added. The maximum dissolved paraffin mass of 50mL of the composite paraffin remover/inhibitor is recorded, and the saturated dissolved paraffin amount is calculated to be 0.59 g/mL.
Example 4
On the basis of the embodiment 2, the embodiment provides a preferable oil well environment-friendly microbial composite paraffin removal and prevention agent.
The strain nutrient solution comprises the following components in percentage by weight: 96kg of deionized water, 32kg of sweet potato powder, 3kg of liquid paraffin, 3kg of vinasse, 2kg of agar, 1.5kg of NaCl and 1.5kg of NH4Cl、5kgKH2PO4、2kgCaCl2、1.5kgNa2SO4。
And compounding 18kg of the paraffin-philic mixed bacterial liquid, 52kg of strain nutrient solution, 7kg of fluorocarbon surfactant FC-3B, 6kg of SP80 and 3kg of n-octanol to obtain the oil well environment-friendly microbial composite paraffin remover and inhibitor.
The oil well environment-friendly microbial composite paraffin removal and prevention agent is subjected to an effect evaluation test, 5g of solid paraffin in an X oil well of a Changqing oil field is taken and soaked in 15mL of the composite paraffin removal and prevention agent, the water bath is kept at the constant temperature of 45 +/-1 ℃, the time for the solid paraffin to be completely dissolved is observed and recorded, and the paraffin dissolving rate of the composite paraffin removal and prevention agent is calculated to be 0.047 g/min.
100g of solid paraffin in an oil well in a Jingan working area of the Changqing oil field is taken, stirred and slowly added into 50mL of the composite paraffin remover and inhibitor, and the mixture is subjected to constant temperature water bath at 55 +/-1 ℃ until the mixture is insoluble within 2 hours after paraffin is added. The maximum dissolved paraffin mass of 50mL of the composite paraffin remover/inhibitor is recorded, and the saturated dissolved paraffin amount is calculated to be 0.56 g/mL.
Example 5
In this example, the wax dissolving rates of the oil well environment-friendly microbial composite paraffin remover and inhibitor of the present invention and a single microbial strain are compared. 5g of solid paraffin in an oil well in a Jingan operation area of the Changqing oil field is taken and respectively soaked in 15mL of the prepared environment-friendly microbial composite paraffin remover and inhibitor for the oil well, the water bath is kept at the constant temperature of 45 +/-1 ℃, and the time for completely dissolving the solid paraffin is observed and recorded. The results of the comparison are shown in Table 2.
TABLE 2 comparison of wax dissolution rates
As shown in Table 2, the wax dissolving rates of Pseudomonas aeruginosa, Bacillus subtilis and Micrococcus terreus are all lower than the wax dissolving rate of the oil well environment-friendly microbial composite wax removing and preventing agent. The oil well environment-friendly microbial composite paraffin remover and inhibitor provided by the invention has the advantages that the common action of various microbes and the wetting and permeation effects of the surfactant increase the paraffin dissolution rate, so that the degradation effect of mixed strains shows better characteristics.
Example 6
The change rule of microbial adsorption of the environment-friendly microbial composite paraffin removal and prevention agent for the oil well prepared in the example 2 is researched. The indoor test method comprises the following steps: at a concentration of 108Adding a plurality of test pieces into the/mL bacterial liquid, placing the bacteria liquid in a shaking table at 50 ℃ to shake at 130r/min to simulate the crude oil flowing condition of an oil well pipeline, taking the test pieces to dye and photograph after the microorganisms are adsorbed and balanced, and calculating the adsorption density to be 4.12 × 104Per cm2The bacterial liquid was diluted 10-fold and 30-fold, and the above experiment was repeated to calculate the adsorption density of the mixed bacterial liquid to be 1.54 × 104Per cm2And 4.78 × 103Per cm2。
According to the experiment, the environment-friendly microbial composite paraffin removal and prevention agent for the oil well has the advantages that the adsorption density of the mixed bacteria liquid is still high along with the production of oil layer fluid, the mixed bacteria liquid has strong anti-scouring capability, the adsorption density cannot be obviously reduced due to the scouring of the oil well fluid, and the action period of the composite paraffin removal and prevention agent is prolonged.
Example 7
The oil well environment-friendly microbial composite paraffin removal and prevention agent (example 3) of the invention is compared with the paraffin removal and prevention effects of commercially available oil-based paraffin removal and prevention agent SM-QL-100 (Shaoxing Shangymo research institute of organic chemistry) and biological paraffin removal and prevention agent KL-1083 (Shaanxi Kaili environmental engineering Co., Ltd.). The test methods refer to the prior art. The test results are shown in Table 3.
TABLE 3 comparative test results of wax removal and prevention effects
As can be seen from Table 3, the oil well environment-friendly microbial composite paraffin inhibitor prepared in example 3 has a higher paraffin dissolution speed, a larger saturated paraffin dissolution amount and a higher crude oil viscosity reduction rate than the commercially available biological paraffin inhibitor KL-1083; compared with the commercially available oil-based paraffin removal and prevention agent SM-QL-100, the petroleum wax removal and prevention agent is safe and easy to transport, does not contain organic solvent, does not damage stratum, is environment-friendly and human-friendly, does not influence the quality of crude oil, and can ensure the normal production of an oil well.
The wax-philic mixed microorganism of the oil well environment-friendly microbial composite paraffin removal and prevention agent prepared by the invention has stronger anti-scouring capability and prolonged paraffin removal action period, and the surfactant is added, so that the affinity of the microorganism and the crude oil is increased, the long-chain alkane in the crude oil is more easily decomposed, and the purposes of paraffin removal and paraffin prevention are achieved. The invention has simple process, no toxicity and environmental protection, and is a high-efficiency and environmental-friendly composite paraffin removal and prevention agent suitable for each operation area.
The present invention has been further described with reference to the examples, but the present invention is not limited to the above-described embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (3)
1. An oil well environment-friendly microbial composite paraffin remover and inhibitor is characterized by comprising 15-20 parts by mass of a paraffin-philic mixed bacterial liquid, 15-60 parts by mass of a strain nutrient solution, 5-9 parts by mass of a first surfactant, 5-9 parts by mass of a second surfactant and 2-4 parts by mass of an intersolunt;
the wax-philic mixed bacterial liquid is a mixed liquid of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus culture fermentation liquid in a volume ratio of 1:2: 1;
the strain nutrient solution comprises, by mass, 20-35 parts of sweet potato powder, 1-5 parts of liquid paraffin, 1-4 parts of vinasse, 1-3 parts of agar, 1-2 parts of sodium chloride, 1-2 parts of ammonium chloride, 2-7 parts of monopotassium phosphate, 1-2 parts of calcium chloride, 1-2 parts of sodium sulfate and 90-100 parts of water;
the first surfactant is one of a fluorocarbon surfactant FC-3B and oleamide betaine FDCH-1;
the second surfactant is one of polyoxyethylene octyl phenol ether-10 and SP 80;
the mutual solvent is one of n-octanol and ethylene glycol monobutyl ether.
2. The oil well environment-friendly type microbial composite paraffin remover and inhibitor as claimed in claim 1, wherein the amount of pseudomonas aeruginosa, bacillus subtilis and micrococcus terreus in the composite paraffin remover and inhibitor is 5-9 × 108one/mL.
3. The use of the environmentally friendly microbial composite paraffin remover/inhibitor for oil wells according to claim 1 in oil field gathering and transportation operations.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113502251A (en) * | 2021-08-11 | 2021-10-15 | 湖北三雄科技发展有限公司 | Preparation method of anti-corrosion wax-control compound microbial agent for oil well exploitation |
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| CN112410008A (en) * | 2020-12-07 | 2021-02-26 | 西安长庆化工集团有限公司 | Biological enzyme treating agent for oil field and preparation method and application thereof |
| CN112410008B (en) * | 2020-12-07 | 2022-12-20 | 西安长庆化工集团有限公司 | Biological enzyme treating agent for oil field and preparation method and application thereof |
| CN113502251A (en) * | 2021-08-11 | 2021-10-15 | 湖北三雄科技发展有限公司 | Preparation method of anti-corrosion wax-control compound microbial agent for oil well exploitation |
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| CN111690392B (en) | 2023-04-14 |
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