CN107939341A - A kind of microbial wax control agent and preparation method thereof - Google Patents
A kind of microbial wax control agent and preparation method thereof Download PDFInfo
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- CN107939341A CN107939341A CN201711220125.5A CN201711220125A CN107939341A CN 107939341 A CN107939341 A CN 107939341A CN 201711220125 A CN201711220125 A CN 201711220125A CN 107939341 A CN107939341 A CN 107939341A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 36
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 29
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- VILCJCGEZXAXTO-UHFFFAOYSA-N 2,2,2-tetramine Chemical compound NCCNCCNCCN VILCJCGEZXAXTO-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229940051841 polyoxyethylene ether Drugs 0.000 claims abstract description 16
- 229920000056 polyoxyethylene ether Polymers 0.000 claims abstract description 16
- 229960001124 trientine Drugs 0.000 claims abstract description 16
- 229920002472 Starch Polymers 0.000 claims abstract description 12
- 235000019698 starch Nutrition 0.000 claims abstract description 12
- 239000008107 starch Substances 0.000 claims abstract description 12
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims description 18
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 abstract description 11
- 238000001556 precipitation Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 10
- 230000008021 deposition Effects 0.000 abstract description 9
- 239000003129 oil well Substances 0.000 abstract description 6
- 150000003839 salts Chemical class 0.000 abstract description 5
- 239000001993 wax Substances 0.000 description 69
- 239000012188 paraffin wax Substances 0.000 description 18
- 239000003921 oil Substances 0.000 description 14
- 235000019198 oils Nutrition 0.000 description 14
- 239000000523 sample Substances 0.000 description 12
- 238000000151 deposition Methods 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 239000010779 crude oil Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 5
- 239000010865 sewage Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 239000003209 petroleum derivative Substances 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000012496 blank sample Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000002734 clay mineral Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019476 oil-water mixture Nutrition 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B37/00—Methods or apparatus for cleaning boreholes or wells
- E21B37/06—Methods or apparatus for cleaning boreholes or wells using chemical means for preventing or limiting, e.g. eliminating, the deposition of paraffins or like substances
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Mining & Mineral Resources (AREA)
- Geology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
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- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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- Microbiology (AREA)
- Fluid Mechanics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- General Life Sciences & Earth Sciences (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The present invention relates to a kind of microbial wax control agent, each component and content are as follows：KCl 30~130kg；NaNO320~80 kg；K2HPO420~40 kg；20 ~ 50 kg of starch；Bacillus licheniformis (Bacillus licheniformis) 20 ~ 50kg of bacterium solution；50 ~ 80 kg of triethylene tetramine polyoxyethylene ether；710 ~ 840 kg of water.The invention further relates to the preparation method of the microbial wax control agent.The microbial wax control agent of the present invention can effectively control the wax deposition of high temperature and high salt oil well, have higher wax removal rate, inhibiting rate for wax precipitation and preferable viscosity break ratio, overall to have the characteristics that highly reliable, using effect is good, high financial profit.
Description
Technical field
The invention belongs to petroleum industry Oilfield Chemicals field, is related to a kind of microbial wax control agent and preparation method thereof.
Background technology
C in crude oil16H34—C63H128N-alkane is known as paraffin.When crude oil is in high temperature and pressure stratum, wax crystalline substance is with liquid
Body form exists, but in recovery process, as temperature and pressure decline and light components constantly escape, the molten wax ability of crude oil
Reduce, paraffin starts to crystallize, separates out, assembles, and constantly growing up and depositing is attached to oil reservoir, oil pipe, casing, sucker rod (pump) and ground
The metal surface of the oil well installations such as face petroleum pipeline, this can seriously affect oil field and normally produce.In order to carry out normal production operation,
Effective Paraffin Removal measure must be taken.Traditional Paraffin Removal technology mainly includes mechanical paraffin removal (such as paraffin scraper), hot wash
(including deep fat is washed, hot water wash etc.), chemical paraffin removing and inhibitor and electrothermal way etc. are added, utilize the above method to carry out Paraffin Removal energy
Certain effect is received, but all there are the shortcomings of operation is frequent, energy consumption is big, costly, pollution stratum and safety and environmental protection performance is poor.
Microorganism Wax Removal And Paraffin Inhibition overcomes the shortcomings that tool wax removal, hot wash, Chemical Paraffin Removal ＆ Control Techniques, excellent with many protrusions
Point：Construction method is simple, and operating cost is low, and does not influence the property of oil product, and any pollution will not be caused to stratum.Microorganism is clear
The petroleum hydrocarbon degradation bacterium Mixed Microbes that wax-proofing agent is made of a variety of anaerobism and facultative anaerobic bacteria.Petroleum hydrocarbon degradation bacterium Mixed Microbes separate
From high-content wax oil well produced liquid, it carries out metabolism using the wax component in crude oil as sole carbon source.Microorganism individual is micro-
Small, cell membrane has special construction, and some surfaces have flagellum, has very strong adhesiveness, and growth and breeding is fast.Microorganism is attached
And soak body surface growth and breeding in metal or clay mineral etc., form one layer of thin and compact hydrophilic and oleophobic microorganism and protect
Cuticula（Such as internal surface of sleeve pipe, sucker rod surface）, have the function that to shield nucleus, prevent wax crystallization, and then play the role of wax control.
But the wax deposition problem of the oil well for high temperature, high salt, conventional microbial method cannot be solved effectively, its Paraffin Removal effect
Fruit is poor, far from the requirement for meeting oil field actual production, it is therefore desirable to further improves.
The content of the invention
The first object of the present invention is to provide a kind of microbial wax control agent, overcomes current microbial method Paraffin Removal technology pair
In the wax deposition problem effect of high temperature, the oil well of high salt is poor the problem of.
The second object of the present invention is to provide the preparation method of mentioned microorganism Wax removing agent.
The present invention is achieved through the following technical solutions：
First, a kind of microbial wax control agent, each component and content are as follows：
KCl：30~130kg：
NaNO3：20~80 kg；
K2HPO4：20~40 kg；
Starch：20 ~50 kg；
Bacillus licheniformis (Bacillus licheniformis) bacterium solution：20~50kg；
Triethylene tetramine polyoxyethylene ether：50~80 kg；
Water：710~840 kg.
Specifically, the molecular weight of the triethylene tetramine polyoxyethylene ether is 3000-8000.
Further, the microbial wax control agent, each component and content are as follows：
KCl：80kg：
NaNO3：60 kg；
K2HPO4：30 kg；
Starch：35 kg；
Bacillus licheniformis (Bacillus licheniformis) bacterium solution：35kg；
Triethylene tetramine polyoxyethylene ether：60 kg；
Water：780 kg.
Specifically, the molecular weight of the triethylene tetramine polyoxyethylene ether is 5000.
Specifically, the culture presevation numbering of the bacillus licheniformis (Bacillus licheniformis) is CICC
No.10094。
2nd, a kind of preparation method of microbial wax control agent according to claim 1, this method include following step
Suddenly：
（1）By KCl, NaNO3、K2HPO4, starch and water be proportionally added into fermentation tank, stir evenly；
（2）It is heated to 125 DEG C to sterilize 30 minutes, is cooled to 30 ~ 55 DEG C；
（3）Bacillus licheniformis (Bacillus licheniformis) bacterium solution is proportionally added into again to fermentation tank, is added in proportion
Enter triethylene tetramine polyoxyethylene ether, ferment at constant temperature 3 ~ 7 days；
（4）It is cooled to room temperature while stirring, up to microbial wax control agent.
Further, this method comprises the following steps：
（1）By KCl, NaNO3、K2HPO4, starch and water be proportionally added into fermentation tank, stir evenly；
（2）It is heated to 125 DEG C to sterilize 30 minutes, is cooled to 30 DEG C；
（3）Bacillus licheniformis (Bacillus licheniformis) bacterium solution is proportionally added into again to fermentation tank, is added in proportion
Enter triethylene tetramine polyoxyethylene ether, ferment at constant temperature 3 days；
（4）It is cooled to room temperature while stirring, up to microbial wax control agent.
Specifically, the culture presevation numbering of the bacillus licheniformis (Bacillus licheniformis) is CICC
No.10094。
The bacillus licheniformis (Bacillus licheniformis) that the present invention uses is Gram-positive bacillus, lichens
The growing environment of micro-organisms bacillus is oil-in-water oil water mixture, and countless tiny microorganism drops are wrapped in countless micro-
Metabolism is carried out around small oil droplet, bacterium solution and its metabolite absorption are on the metal surfaces such as well letter, oil pipe bar, so as to reach
To wax deposition is prevented, extend the purpose for phase of being excused from a college course.Microorganism has paraffin and decomposes and swallow again using paraffin hydrocarbon as carbon source Nutrient medium
Act on and play the role of wax removal.
Using the good effect of above-mentioned technical proposal：The microbial wax control agent of the present invention can effectively control high temperature and high salt oily
The wax deposition of well, has higher wax removal rate, inhibiting rate for wax precipitation and a preferable viscosity break ratio, and overall have that highly reliable, using effect is good, warp
The characteristics of high efficiency of helping.
Embodiment
The source of biomaterial in the present invention：
1st, bacillus licheniformis (Bacillus licheniformis) CICC No.10094：Purchased from Chinese industrial microbial bacteria
Kind preservation administrative center.
Technical scheme is described further with reference to specific embodiment and comparative example, but should not be managed
Solve as limitation of the present invention：
Embodiment 1
By 130kgKCl, 20kgNaNO3、20kgK2HPO4, 50kg starch and 710kg water is proportionally added into stirring, temperature control
And in the fermentation tank of vacuum system, stir evenly, it is heated to 125 DEG C and sterilizes 30 minutes, is cooled to 55 DEG C, then be proportionally added into bud
The gram-positive bacterium bacterium bacterium solution 50kg of spore adds 50kg triethylene tetramine polyoxyethylene ether to fermentation tank（Molecular weight
5000）, ferment at constant temperature is after 7 days.It is cooled to room temperature while stirring, that is, obtains required microbial wax control agent.
Embodiment 2
By 30kgKCl, 80kgNaNO3、40kgK2HPO4, 20kg starch and 840kg water is proportionally added into stirring, temperature control
And in the fermentation tank of vacuum system, stir evenly, it is heated to 125 DEG C and sterilizes 30 minutes, is cooled to 45 DEG C, then be proportionally added into bud
The gram-positive bacterium bacterium bacterium solution 20kg of spore adds 80kg triethylene tetramine polyoxyethylene ether to fermentation tank（Molecular weight
5000）, ferment at constant temperature is after 5 days.It is cooled to room temperature while stirring, that is, obtains required microbial wax control agent.
Embodiment 3
By 80kgKCl, 60kgNaNO3、30kgK2HPO4, 35kg starch and 780kg water is proportionally added into stirring, temperature control
And in the fermentation tank of vacuum system, stir evenly, it is heated to 125 DEG C and sterilizes 30 minutes, is cooled to 30 DEG C, then be proportionally added into bud
The gram-positive bacterium bacterium bacterium solution 35kg of spore adds 60kg triethylene tetramine polyoxyethylene ether to fermentation tank（Molecular weight
5000）, ferment at constant temperature is after 3 days.It is cooled to room temperature while stirring, that is, obtains required microbial wax control agent.
Experimental example 1
This experimental example is used for the effect for illustrating microbial wax control agent.
Indoor Paraffin Removal rate evaluation method：
(1) it is buckled to the wax removal rate of bottle method measure microbial wax control agent
1. taking the wide-mouth bottle 5 of 100mL clean dries and numbering being 1,2,3,4,5 respectively, wherein 1 is used as blank, wide-mouth is weighed
Bottle quality (is recorded as m0), 2,3,4,5 are used as test sample, weigh wide-mouth bottle quality and (are recorded as m1)。
2. the water-free oil oil sample after heating is poured into 5 bottles respectively, shaking wide-mouth bottle makes oil sample uniformly cover
On the inside of sample bottle, unnecessary oil sample is poured out, cooled and solidified, weighs blank wide-mouth bottle quality and (be recorded as m respectively01), test sample
Wide-mouth bottle quality (is recorded as m11)。
3. adding the water sample of the crude oil Produced Liquid abjection of 100g in blank group wide-mouth bottle, it is separately added into experiment wide-mouth bottle
100mL scenes sewage adds concentration as 5% microbial wax control bacterium solution, the 5%KJ-01 for implementing 1,5% implementation, 2,5% implementation 3（City
Sell）Microbial wax control agent, 5 wide-mouth bottles are put into 45 ± 1 DEG C of water-bath after constant temperature 48h and are taken out.
4. the liquid in 5 wide-mouth bottles is poured out, it is dry, blank group wide-mouth bottle quality is weighed respectively (is recorded as m02), examination
Test sample wide-mouth bottle quality and (be recorded as m12)。
5. the calculating of wax removal rate：
Wax removal rate calculation formula：E=(△ M1-△M2)/△M1×100
In formula：E-wax removal rate, is expressed as a percentage.
△M1The average value of-blank group wax removal amount, unit are gram (g), i.e. blank bottle (m01-m0)-(m01-m02) value.
△M2The average value of the wax removal amount of-test group, unit are gram (g), i.e. assay flask (m11-m1)-(m11-m12) value.
(2) inhibiting rate for wax precipitation of hanging slice method measure microbial wax control agent
1. take the wide-mouth bottle 5 of 100mL clean dries and numbering is 1,2,3,4,5,1 be used as blank respectively, weighing wide-mouth bottle matter
Amount (is recorded as m0), another group 2,3,4,5 is used as test group, weighs wide-mouth bottle quality and (is recorded as m1).It is wide to above-mentioned 5 respectively
Mouth bottle adds the water-free oil oil sample of 40 ± 0.1g.
2. blank group wide-mouth bottle adds the live water sample of 40mL, it is real for 5% to be separately added into 40mL concentration to test group wide-mouth bottle
Apply microbial wax control bacterium solution, 5%KJ-01 that 1,5% implementation 2,5% implements 3（It is commercially available）Microbial wax control agent.
3. above-mentioned 5 wide-mouth bottles are put into constant temperature 30 minutes in 45 ± 1 DEG C of water-bath.
4. by above-mentioned 5 wide-mouth bottles hand fully shaking 200 times, and ensuring firmly consistent, it is allowed to uniformly mixed, then
Put back in 40 ± 1 DEG C of water-bath and taken out after constant temperature 48h.
5. wide-mouth bottle is buckled in a receiver in insulating box (40 ± 1 DEG C), constant temperature makes wide-mouth bottle no longer in 60 minutes
Oil dripping, then takes out wax deposition wide-mouth bottle, weighs the wide-mouth bottle quality that oily wax glues wall, and blank sample and test sample are recorded as respectively
m01And m12。
6. the calculating of inhibiting rate for wax precipitation：
Inhibiting rate for wax precipitation calculation formula：E=(△ M1-△M2)/△M1×100
In formula：E-inhibiting rate for wax precipitation, is expressed as a percentage.
△M1The value of-blank wax deposition amount, unit are gram (g), i.e. blank bottle m0-m01Value.
△M2The value of the wax deposition amount of-test group, unit are gram (g), i.e. test sample m1-m12Value.
(3) measure of viscosity break ratio
1. taking 200 g of crude oil, it is respectively charged into 250 mL wide-mouth bottles, 1,2,3,4,5,1 is used as blank, is placed in 45 DEG C of constant temperature, and 2,
3,4,5, it is separately added into 10g and implements 1,10g implementations 2, the microbial wax control bacterium solution of 10g implementations 3,10g KJ-01（It is commercially available）, stir
15min is mixed, is taken out in 45 ± 1 DEG C of water-bath after constant temperature 48h.
2. measure viscosity of crude with cloth viscosimeter.
3. the calculating of viscosity break ratio：
μ=η0-η1/η0
η0The viscosity of the non-dosing of blank
η1Viscosity after dosing
Embodiment 1 ~ 3 and the experimental result of KJ01 are as shown in Table 1 and Table 2：
The Paraffin Removal rate of 1 embodiment 1 ~ 3 of table and KJ01
Its wax removal rate of the microbial wax control bacterium solution of embodiment 1 ~ 3 can be higher than 40%, and inhibiting rate for wax precipitation reaches more than 90%, its
In, it is optimal using 3 effect of embodiment.
The viscosity break ratio of 2 embodiment 1 ~ 3 of table and KJ01
The microbial wax control bacterium solution of embodiment 1 ~ 3 reduces the viscosity of crude oil to a certain extent, its viscosity break ratio 40% or so, with
3 effect of embodiment is optimal.
Experimental example 2
This experimental example illustrates embodiment 3 compared with commercially available KJ-01 microbial wax controls agent Paraffin Removal, viscosity reducing effect.
Embodiment 3 is placed under 70 DEG C of environment after 24h with the commercially available KJ-01 microbial wax controls agent of grand celebration, is fallen according to above-mentioned (1)
Detain the wax removal rate method measure inhibiting rate for wax precipitation of bottle method measure microbial wax control agent, sewage 2.0%NaCl, 0.3%KCl, 0.03%
Na2CO3、0.1%NaHCO3The simulation water of configuration replaces Produced Liquid sewage；Drop is measured according to the assay method of above-mentioned (2) viscosity break ratio
Viscous rate.
The Paraffin Removal rate of 3 embodiment of table and KJ-01
The microbial wax control bacterium solution of embodiment 3 is after high-temperature process, its wax removal rate reaches in high salinity sewage
43.67%, and KJ-01 wax removal rates are only 13.41%；2 inhibiting rate for wax precipitation of embodiment reaches 90.36%, and KJ-01 inhibiting rate for wax precipitation is only
36.21%。
The viscosity break ratio of 4 embodiment of table and KJ-01
The microbial wax control bacterium solution of embodiment 3 is after high-temperature process, and viscosity break ratio reaches 42.56%, and KJ-01 viscosity break ratios are only
For 19.71%.
The microbial wax control agent of the present invention can effectively control the wax deposition of high temperature and high salt oil well, have higher wax removal rate,
Inhibiting rate for wax precipitation and preferable viscosity break ratio, it is overall to have the characteristics that highly reliable, using effect is good, high financial profit.
Claims (8)
- A kind of 1. microbial wax control agent, it is characterised in that：Each component and content are as follows：KCl：30~130kg；NaNO3：20~80 kg；K2HPO4：20~40 kg；Starch：20 ~50 kg；Bacillus licheniformis (Bacillus licheniformis) bacterium solution：20~50kg；Triethylene tetramine polyoxyethylene ether：50~80 kg；Water：710~840 kg.
- 2. microbial wax control agent according to claim 1, it is characterised in that：The triethylene tetramine polyoxyethylene ether Molecular weight be 3000-8000.
- 3. microbial wax control agent according to claim 1, it is characterised in that：Each component and content are as follows：KCl：80kg；NaNO3：60 kg；K2HPO4：30 kg；Starch：35 kg；Bacillus licheniformis (Bacillus licheniformis) bacterium solution：35kg；Triethylene tetramine polyoxyethylene ether：60 kg；Water：780 kg.
- 4. microbial wax control agent according to claim 3, it is characterised in that：The triethylene tetramine polyoxyethylene ether Molecular weight be 5000.
- 5. the microbial wax control agent according to claim 1 or 3, it is characterised in that：The bacillus licheniformis The culture presevation numbering of (Bacillus licheniformis) is CICC No.10094.
- A kind of 6. preparation method of microbial wax control agent according to claim 1, it is characterised in that：This method include with Lower step：（1）By KCl, NaNO3、K2HPO4, starch and water be proportionally added into fermentation tank, stir evenly；（2）It is heated to 125 DEG C to sterilize 30 minutes, is cooled to 30 ~ 55 DEG C；（3）Bacillus licheniformis (Bacillus licheniformis) bacterium solution is proportionally added into again to fermentation tank, is added in proportion Enter triethylene tetramine polyoxyethylene ether, ferment at constant temperature 3 ~ 7 days；（4）It is cooled to room temperature while stirring, up to microbial wax control agent.
- 7. the preparation method of microbial wax control agent according to claim 6, it is characterised in that：This method includes following step Suddenly：（1）By KCl, NaNO3、K2HPO4, starch and water be proportionally added into fermentation tank, stir evenly；（2）It is heated to 125 DEG C to sterilize 30 minutes, is cooled to 30 DEG C；（3）Bacillus licheniformis (Bacillus licheniformis) bacterium solution is proportionally added into again to fermentation tank, is added in proportion Enter triethylene tetramine polyoxyethylene ether, ferment at constant temperature 3 days；（4）It is cooled to room temperature while stirring, up to microbial wax control agent.
- 8. the preparation method of the microbial wax control agent according to claim 6 or 7, it is characterised in that：The lichens bud The culture presevation numbering of born of the same parents bacillus (Bacillus licheniformis) is CICC No.10094.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111574980A (en) * | 2020-06-19 | 2020-08-25 | 陕西凯利清洗有限公司 | Microbial paraffin removal and prevention agent and use method and preparation method thereof |
| CN111690392A (en) * | 2020-07-15 | 2020-09-22 | 陕西丰登石化有限公司 | Environment-friendly microbial composite paraffin removal and prevention agent for oil well |
| CN114320276A (en) * | 2020-09-30 | 2022-04-12 | 中国石油天然气股份有限公司 | Method and device for determining wax removal and prevention effect through microorganisms |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO1992015771A1 (en) * | 1991-03-04 | 1992-09-17 | Archaeus Technology Group Ltd | Enhanced oil recovery |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111574980A (en) * | 2020-06-19 | 2020-08-25 | 陕西凯利清洗有限公司 | Microbial paraffin removal and prevention agent and use method and preparation method thereof |
| CN111690392A (en) * | 2020-07-15 | 2020-09-22 | 陕西丰登石化有限公司 | Environment-friendly microbial composite paraffin removal and prevention agent for oil well |
| CN111690392B (en) * | 2020-07-15 | 2023-04-14 | 陕西丰登石化有限公司 | Environment-friendly microbial composite paraffin removal and prevention agent for oil well |
| CN114320276A (en) * | 2020-09-30 | 2022-04-12 | 中国石油天然气股份有限公司 | Method and device for determining wax removal and prevention effect through microorganisms |
| CN114320276B (en) * | 2020-09-30 | 2023-09-26 | 中国石油天然气股份有限公司 | Method and device for determining wax removal and prevention effects by microorganisms |
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