CN111019748A - Biological stabilizer for inhibiting metal processing liquid from putrefaction and preparation method and application thereof - Google Patents
Biological stabilizer for inhibiting metal processing liquid from putrefaction and preparation method and application thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M173/00—Lubricating compositions containing more than 10% water
- C10M173/02—Lubricating compositions containing more than 10% water not containing mineral or fatty oils
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M177/00—Special methods of preparation of lubricating compositions; Chemical modification by after-treatment of components or of the whole of a lubricating composition, not covered by other classes
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2207/00—Organic non-macromolecular hydrocarbon compounds containing hydrogen, carbon and oxygen as ingredients in lubricant compositions
- C10M2207/02—Hydroxy compounds
- C10M2207/021—Hydroxy compounds having hydroxy groups bound to acyclic or cycloaliphatic carbon atoms
- C10M2207/022—Hydroxy compounds having hydroxy groups bound to acyclic or cycloaliphatic carbon atoms containing at least two hydroxy groups
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2207/00—Organic non-macromolecular hydrocarbon compounds containing hydrogen, carbon and oxygen as ingredients in lubricant compositions
- C10M2207/08—Aldehydes; Ketones
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2215/00—Organic non-macromolecular compounds containing nitrogen as ingredients in lubricant compositions
- C10M2215/02—Amines, e.g. polyalkylene polyamines; Quaternary amines
- C10M2215/04—Amines, e.g. polyalkylene polyamines; Quaternary amines having amino groups bound to acyclic or cycloaliphatic carbon atoms
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2215/00—Organic non-macromolecular compounds containing nitrogen as ingredients in lubricant compositions
- C10M2215/02—Amines, e.g. polyalkylene polyamines; Quaternary amines
- C10M2215/04—Amines, e.g. polyalkylene polyamines; Quaternary amines having amino groups bound to acyclic or cycloaliphatic carbon atoms
- C10M2215/042—Amines, e.g. polyalkylene polyamines; Quaternary amines having amino groups bound to acyclic or cycloaliphatic carbon atoms containing hydroxy groups; Alkoxylated derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2215/00—Organic non-macromolecular compounds containing nitrogen as ingredients in lubricant compositions
- C10M2215/22—Heterocyclic nitrogen compounds
- C10M2215/221—Six-membered rings containing nitrogen and carbon only
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2219/00—Organic non-macromolecular compounds containing sulfur, selenium or tellurium as ingredients in lubricant compositions
- C10M2219/10—Heterocyclic compounds containing sulfur, selenium or tellurium compounds in the ring
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10M—LUBRICATING COMPOSITIONS; USE OF CHEMICAL SUBSTANCES EITHER ALONE OR AS LUBRICATING INGREDIENTS IN A LUBRICATING COMPOSITION
- C10M2227/00—Organic non-macromolecular compounds containing atoms of elements not provided for in groups C10M2203/00, C10M2207/00, C10M2211/00, C10M2215/00, C10M2219/00 or C10M2223/00 as ingredients in lubricant compositions
- C10M2227/06—Organic compounds derived from inorganic acids or metal salts
- C10M2227/061—Esters derived from boron
Abstract
The invention provides a biological stabilizer for inhibiting metal processing liquid from decaying, and a preparation method and application thereof, wherein the biological stabilizer for inhibiting metal processing liquid from decaying comprises the following components in parts by weight: 20-30 parts of organic carbon, 10-30 parts of organic nitrogen, 5-10 parts of growth factors, 20-30 parts of balancing agents, 1-5 parts of protobacteria powder thalli and a proper amount of water. The biological stabilizer can be introduced into a processing liquid formula or added at the edge of a field machine tool groove, has good compatibility with various processing liquids, can reduce or eliminate the use of bactericides, does not influence the use performance of the processing liquid, is environment-friendly, does not have adverse effect on operators, and can remarkably prolong the service cycle of the processing liquid.
Description
Technical Field
The invention belongs to the technical field of metal working fluids, and particularly relates to a biological stabilizer for inhibiting metal working fluid from decaying, and a preparation method and application thereof.
Background
Metal working fluid spoilage is caused primarily by the proliferation of a large number of harmful microorganisms in the system. The base oil and certain additives in the processing fluids are nutrients upon which microorganisms depend for survival. When bacteria multiply in a large quantity, the pH value of the processing liquid is reduced, and an unpleasant smell is generated; when the fungi are propagated in large quantities, the processing liquid can generate lumps which are easy to block the circulating pipeline and the filter screen of the machine tool. The decomposition of the effective components in the working fluid by these microorganisms causes the reduction of the lubricity, rust prevention, stability and other properties of the working fluid, causes abrasion and corrosion to machines, workpieces and tools, and affects the physical health of operators.
Currently, the most common method of inhibiting microbial growth in metalworking fluids is the addition of chemical biocides. The main types of the bactericide include formaldehyde condensates, isothiazolinones, phenols, morpholine compounds, pyridine derivatives, amine borates, and the like. Effect of chemical fungicides on microorganisms: firstly, the metabolic activity of microorganisms is inhibited, so that the bacterial action is inhibited; secondly, the microbial metabolism mechanism or the thallus structure is destroyed, and the sterilization effect is achieved.
With the increasing concern of people on health safety, environmental sanitation and health safety regulations are becoming stricter. Although effective, the addition of chemical disinfectants can seriously harm the environment and the health of users.
Disclosure of Invention
In view of the above, the present invention aims to provide a biostabilizer for inhibiting metal processing liquid from putrefaction, so as to overcome the defects of the prior art, introduce a processing liquid formula or add the processing liquid at the edge of a field machine tool groove, have good compatibility with various processing liquids, reduce or eliminate the use of bactericides, have no influence on the use performance of the processing liquid, be environment-friendly, have no adverse effect on operators, and significantly prolong the service cycle of the processing liquid.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
the biological stabilizer for inhibiting the corrosion of the metal processing liquid comprises the following components in parts by weight: 20-30 parts of organic carbon, 10-30 parts of organic nitrogen, 5-10 parts of growth factors, 20-30 parts of balancing agents, 1-5 parts of protobacteria powder thalli and a proper amount of water.
Wherein, the addition amount of water is based on the principle that the microbial stabilizer maintains a stable and transparent state and achieves good flowing performance, and the addition amount of water is generally 5-15 parts by weight, preferably 5-10 parts by weight.
Preferably, the water is sterilized distilled water.
Preferably, the organic carbon is one or more of glycerol, fatty alcohol polyether, tetrapolyricinoleate and acidic phosphate.
Preferably, the organic carbon is glycerol. The inventors have found that glycerol provides the carbon source for the protists which is needed for rapid propagation, while it is difficult for the harmful microorganisms in the process fluid to metabolize glycerol as a carbon source.
Preferably, the organic nitrogen is two or more of diethanolamine, triethanolamine, diglycolamine and borate.
Preferably, the organic nitrogen is diethanolamine and boric acid ester according to the mass ratio (1 +/-0.5): (2. + -. 0.5). The inventor finds that diethanolamine and boric acid ester are used as nitrogen sources, which are beneficial to the rapid propagation of the protozoite and can inhibit the growth of harmful microorganisms.
Preferably, the growth factor is one or more of a base, purine, pyrimidine, biotin and nicotinic acid.
Preferably, the growth factor is biotin and/or nicotinic acid. The inventors have discovered that biotin and nicotinic acid-based growth factors promote the growth of probiotics in biostabilizers while at the same time somewhat inhibiting harmful microorganisms in the process fluids.
Preferably, the balancing agent is one or more than two of sodium glutamate, sodium alginate, sucrose, corn flour, cake flour, bran, turf, lactose, galactose and arabinose.
Preferably, the balancing agent is sodium glutamate or/and galactose. The inventor finds that sodium glutamate or galactose as a balancing agent can maintain the biomass of the probiotics in the metal processing liquid in a proper interval to a certain extent, and can not excessively propagate to influence the performance of the processing liquid, and simultaneously inhibit harmful microorganisms.
Preferably, the protozoa is one or more than two of trichoderma, streptomyces, chaetomium, pseudomonas and bacillus.
Preferably, the balancing agent is at least one of trichoderma and pseudomonas. The inventor carries out flora analysis on a large amount of metal processing liquid which has relatively long service period and good performance maintenance, finds that the metal processing liquid contains some common harmless flora and the harmless flora has absolute advantages on biomass, and separates, cultures and collects the harmless flora and is called protobacteria. The inventor finds that the microorganism can produce enzyme substances and secondary metabolites, and degrade cell walls of harmful microorganisms in processing liquid; through rapid propagation in the processing liquid, the advantages are achieved, the living space of harmful microorganisms is squeezed, so that the lubricating and antirust effective components in the processing liquid are not degraded by the harmful microorganisms or the degradation rate is slowed down, and the service life is prolonged.
Another object of the present invention is to provide a method for producing a biostabilizer for inhibiting putrefaction of a metal working fluid, so as to produce the biostabilizer.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a method of preparing a biostabilizer for inhibiting corrosion of a metalworking fluid, comprising the steps of:
(1) stirring and dissolving organic carbon and organic nitrogen in a formula amount at 50-70 ℃ until a clear and transparent oily substance A is obtained;
(2) stirring the water and the balancing agent in the formula amount at 30-35 ℃ until the water and the balancing agent are completely dissolved to obtain clear transparent liquid B;
(3) when A is cooled to 30-35 ℃, B is added into A and stirred until the B is completely dissolved to form clear transparent liquid C;
(4) adding the growth factor and the protobacteria powder thalli with the formula amount into the C, and continuously stirring until the mixture is uniform to obtain the biological stabilizer.
Preferably, in the step (4), the method for preparing the powder of the protozoan bacteria comprises the following steps:
s1: extracting activated protobacteria, inoculating the extracted activated protobacteria into a proper amount of a protobacteria liquid culture medium, and performing amplification culture twice to obtain a bacterial liquid;
s2: collecting bacteria liquid, centrifuging, removing supernatant, washing the precipitated bacteria with 0.9% sterile normal saline, centrifuging, and removing supernatant to obtain precipitate, i.e. protobacteria bacteria;
s3: and fully drying the thallus of the protobacteria to obtain the powder thallus of the protobacteria.
Preferably, in step S1, the two times of scale-up culture are performed at 20-40 ℃ for 1-5 days.
Preferably, in step S1, the number of colonies of the activated Protista is 107-109The dosage volume ratio of the activated protobacteria to the liquid culture medium is 1: 100.
Preferably, in step S3, the drying temperature is 20-40 ℃.
The invention also relates to the use of a biostabilizer as described above in a metal working fluid and/or machining.
Compared with the prior art, the biological stabilizer for inhibiting the metal processing liquid from decaying has the following advantages:
(1) the microbial balance technology comprises the following steps: when the metal working fluid is used in the metal working fluid, the metal working fluid can be inhibited from putrefaction by adopting a biological control technology of 'bacteria inhibition', a chemical bactericide is not contained, and the living space of harmful microorganisms is occupied by the rapid proliferation of protobacteria, so that the growth of the harmful microorganisms is inhibited, and the service life of the metal working fluid is prolonged.
The biological control technique for suppressing the corrosion of a metal working fluid described above is to add a microbial stabilizer to the working fluid to suppress the corrosion of the metal working fluid. The microbial stabilizer mainly comprises organic carbon, organic nitrogen, growth factors, a balancing agent and probiotics, and does not contain a bactericide. The protobacteria belong to natural harmless flora. Through carrying out flora analysis on a large amount of metal working fluid which has relatively long service cycle and good performance maintenance, the metal working fluid is found to contain some common harmless flora and has absolute advantage on biomass, namely protobacteria; organic carbon and organic nitrogen provide carbon source and nitrogen source for the protobacteria; the growth factor can promote the growth of protobacteria, quickly increase the value in the metal processing liquid, occupy absolute dominant quantity, and compress the living space of harmful microorganisms, thereby inhibiting the growth and propagation of the harmful microorganisms and further inhibiting the putrefaction of the metal processing liquid. The biological control technology can effectively enhance the service cycle of the metal working fluid, reduce or even eliminate the use of chemical bactericides and effectively protect the environment and the human health of users.
(2) Safety and environmental protection: the bactericide is not contained, and the harm to the environment and operators is reduced by inhibiting the growth of harmful microorganisms through the probiotics.
(3) The application is wide: the introduction of a processing liquid formula or on-site machine tool groove edge addition is particularly suitable for various water-based metal processing liquid systems such as emulsion type, full-synthetic type and the like, and the service life of the metal processing liquid is prolonged without influencing the properties of the metal processing liquid.
The preparation method of the biological stabilizer for inhibiting the metal processing liquid from decaying has the same advantages of the biological stabilizer for inhibiting the metal processing liquid from decaying compared with the prior art, and the details are not repeated herein.
Drawings
FIG. 1 shows pH fluctuations in emulsified metal working fluids for examples and comparative examples;
FIG. 2 shows the pH fluctuations of the examples and comparative examples in fully synthetic metalworking fluids.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The present invention will be described in detail with reference to the following examples and accompanying drawings.
First, example 1, 2 and comparative example 1, 2 raw material source
Galactose, biotin, nicotinic acid, glycerol and sodium glutamate are all the alatin reagents.
Diethanolamine and boric acid ester are selected from petroleum chemical plant of Haian of Jiangsu province.
The liquid medium is liquid LB medium, purchased from Beijing spectral analysis science and technology Limited.
Trichoderma harzianum (T. trichoderma harzianum) with the number of BNCC336568 is purchased from northern Nami organisms.
Pseudomonas is selected from Pseudomonas aeruginosa with BNCC336458, and purchased from North Navy organisms.
Second, preparation method
1. Preparation of Protozoa powder thallus
The preparation of the protobacteria powder thallus comprises the following steps:
s1: extracting activated Protozoa, inoculating into appropriate amount of Protozoa liquid culture medium (volume ratio of activated Protozoa to liquid culture medium is 1:100, and each ml of activated Protozoa contains 107-109Bacterial colonies of the protobacteria) are subjected to amplification culture for 1 to 5 days at the temperature of between 20 and 40 ℃, and a bacterial liquid is obtained after two times of amplification culture;
s2: collecting bacteria liquid, centrifuging, removing supernatant, washing the precipitated bacteria with 0.9% sterile normal saline, centrifuging, and removing supernatant to obtain precipitate, i.e. protobacteria bacteria;
s3: and fully drying the thallus of the protobacteria at about 20-40 ℃ to obtain the powder thallus of the protobacteria.
TABLE 1 selection of conditions for preparation of raw powder thalli in examples 1 and 2 and comparative examples 1 and 2 biostabilizers
2. Preparation of biostabilizers
The preparation of the biostabilizer comprises the following steps:
(1) sequentially adding organic carbon and organic nitrogen in a formula amount into a reaction kettle, heating to 50-70 ℃, and stirring to dissolve until a clear and transparent oily substance A is obtained;
(2) stirring the water and the balancing agent in the formula amount in a reaction kettle at the temperature of 30-35 ℃ until the water and the balancing agent are completely dissolved to obtain clear transparent liquid B;
(3) when A is cooled to 30-35 ℃, B is added into A and stirred until the B is completely dissolved to form clear transparent liquid C;
(4) adding the growth factor and the strain of the raw bacterium powder into the C according to the formula amount, and continuously stirring until the mixture is uniform to obtain the biological stabilizer.
Table 2 examples 1, 2 and comparative examples 1, 2 selection of process conditions for the preparation of biostabilizers
It should be noted that, when the biostabilizer is prepared by referring to the preparation method of the biostabilizer of the present invention, the biostabilizers of comparative examples 1 and 2 can be prepared by adding or subtracting raw materials according to the actual formulation.
Third, biostabilizers of examples 1 and 2 and comparative examples 1 and 2
The powder cells of the protozoa and the biostabilizers of examples 1 and 2 and comparative examples 1 and 2 were prepared according to the above-mentioned methods, and the compositions of the biostabilizers described in examples 1 and 2 and comparative examples 1 and 2 are shown in Table 3.
Table 3 composition of the biostabilizers described in examples 1, 2 and comparative examples 1, 2
Fourth, detection method
(1) Each application example and comparative example (i.e., the emulsion type metal working fluid of example 1-1, comparative example 1-1, etc., to which one of the biostabilizers of examples 1, 2 and comparative examples 1, 2 was added, and the fully synthetic type metal working fluid of example 2-1, comparative example 2-1, etc.) of the biostabilizers was diluted to 5 wt% with deionized water, respectively;
(2) adding 5 wt% of corresponding corrosion liquid (cutting corrosion liquid) into each of the examples and the comparative examples, adding 1 wt% of cast iron scraps, placing the mixture in a water bath kettle at 35 ℃, introducing sufficient air (the oxygen pump is used for introducing air, and the oxygen pump is an oxygen pump of Songbao/SOBO and model SB-988, which are produced by Songbao electric appliances Limited of Zhongshan city), specifically, 7d is used as 1 period, 5d of air is introduced in each period, 2d of air is stopped, and sterile water is supplemented in the process to supplement loss caused by evaporation;
(3) the pH value is measured periodically until the test is finished in 90 days;
(4) and finally, testing the antirust performance of the metal working fluid by referring to GB/T6144-2010.
Fifth, result analysis
1. Application of biological control technology in emulsified metal working fluid
Example 1-1: the biocide component was removed from the ground emulsified metal working fluid TSIcut-Emu1000Ti and 3% (wt) of the biostabilizer described in example 1 was added.
Examples 1 to 2: the biocide component was removed from the ground emulsified metal working fluid TSIcut-Emu1000Ti and 3% (wt) of the biostabilizer described in example 2 was added.
Comparative example 1-1: the biocide component was removed from the ground emulsified metalworking fluid TSIcut-Emu1000Ti and 3% by weight of the biostabilizer described in comparative example 1 was added.
Comparative examples 1 to 2: the biocide component was removed from the ground emulsified metalworking fluid TSIcut-Emu1000Ti and 3% (wt) of the biostabilizer described in comparative example 2 was added.
Comparative examples 1 to 3: the biocide component was removed from the grinding emulsion type metalworking fluid TSIcut-Emu1000 Ti.
Comparative examples 1 to 4: self-grinding emulsified metal working fluid TSIcut-Emu1000Ti (containing bactericide).
The examples and comparative examples were tested using the test detection method described above. The results are shown in FIG. 1 and Table 4.
The growth of harmful bacteria in the metal working fluid can decompose the base number storage agent in the metal working fluid, and the metabolite of the harmful bacteria is generally acidic, so that the pH value of the metal working fluid is continuously reduced. As can be seen from FIG. 1, examples 1-1 and 1-2 have better pH stability than comparative examples 1-1, 1-2 and 1-3, and the test was carried out until day 90, and the pH was still higher. In addition, the pH stability of examples 1-1 and 1-2 is superior to that of comparative examples 1-4 containing a bactericide.
TABLE 4 antirust property of single sheet of emulsified metal working fluid for examples and comparative examples
Harmful microorganisms metabolize to generate acidic substances such as organic acid and the like, so that metal corrosion is easily caused, and meanwhile, the breeding of harmful bacteria can decompose and consume the antirust agent, so that the antirust performance of the machining liquid is reduced. As shown in Table 4, in terms of the rust inhibitive performance of the single sheets, comparative examples 1-1, 1-2 and 1-3 had a grade D of rust inhibitive performance by 90 days of the test, and examples 1-1 and 1-2 could maintain the grade A of the single sheets at 90 days and were superior to comparative examples 1-4 containing the bactericide. The experimental results show that: in the emulsified metal working fluid, the biological stabilizer can effectively inhibit the metabolism of harmful microorganisms and the decomposition of the antirust agent.
The combination of the pH value and the antirust property shows that the biostabilizer can be used as an additive to be added into the emulsified metal working fluid and can well maintain the biostability of the working fluid, wherein the growth factor in the biostabilizer component is particularly important.
2. Application of biological control technology in fully synthetic metal working fluid
Example 2-1: the biocide component was removed from the ground fully synthetic metalworking fluid TSIcut-Syn1021S and 3 wt% of the biostabilizer described in example 1 was added.
Example 2-2: the biocide component was removed from the ground fully synthetic metalworking fluid TSIcut-Syn1021S and 3 wt% of the biostabilizer described in example 2 was added.
Comparative example 2-1: the biocide component was removed from the ground fully synthetic metalworking fluid TSIcut-Syn1021S and 3 wt% of the biostabilizer described in comparative example 1 was added.
Comparative examples 2 to 2: the biocide component was removed from the ground fully synthetic metalworking fluid TSIcut-Syn1021S and 3 wt% of the biostabilizer described in comparative example 2 was added.
Comparative examples 2 to 3: the sterilant component was removed from the grinding full synthetic metalworking fluid TSIcut-Syn 1021S.
Comparative examples 2 to 4: self-developed fully synthetic metalworking fluid TSIcut-Syn1021S (containing a biocide).
The examples and comparative examples were tested using the test detection method described above. The results are shown in FIG. 2 and Table 5.
As can be seen from FIG. 2, examples 2-1 and 2-2 using the biocontrol technology of the present invention have better pH stability than comparative examples 2-1, 2-2 and 2-3. Example 1 by 90 days, a higher pH was maintained. In addition, the pH stability of examples 2-1 and 2-2 was superior to that of comparative examples 2-4 containing a bactericide.
TABLE 5 one-piece rust inhibitive Properties of examples and comparative examples in fully synthetic metal working fluids
As shown in Table 5, in terms of the rust inhibitive performance of the single sheets, comparative examples 2-1, 2-2 and 2-3 were rated D in the rust inhibitive performance by 90 days of the test, and examples 2-1 and 2-2 maintained good rust inhibitive performance, were rated A, and were superior to comparative examples 2-4 containing the bactericide. The experimental results show that: in the fully synthetic metal working fluid, the biological stabilizer can effectively inhibit the metabolism of harmful microorganisms and the decomposition of the antirust agent.
The combination of the pH value and the antirust property shows that the biostabilizer can be used as an additive to be added into the fully synthetic metal processing fluid and can well maintain the biostability of the processing fluid, wherein the growth factor in the biostabilizer component is critical.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A biostabilizer for inhibiting corrosion in a metalworking fluid, comprising: the paint comprises the following components in parts by weight: 20-30 parts of organic carbon, 10-30 parts of organic nitrogen, 5-10 parts of growth factors, 20-30 parts of balancing agents, 1-5 parts of protobacteria powder thalli and a proper amount of water.
2. The biostabilizer for inhibiting spoilage of metal processing fluids according to claim 1, wherein: the organic carbon is one or more than two of glycerol, fatty alcohol polyether, tetrapolyricinoleate and acidic phosphate;
preferably, the organic carbon is glycerol;
preferably, the content of water is 5 to 15 parts by weight;
more preferably, the water is sterilized distilled water.
3. The biostabilizer for inhibiting spoilage of metal processing fluids according to claim 1, wherein: the organic nitrogen is two or more of diethanolamine, triethanolamine, diglycolamine and borate;
preferably, the organic nitrogen is diethanolamine and boric acid ester according to the mass ratio (1 +/-0.5): (2. + -. 0.5).
4. The biostabilizer for inhibiting spoilage of metal processing fluids according to claim 1, wherein: the growth factor is one or more than two of basic group, purine, pyrimidine, biotin and nicotinic acid;
preferably, the growth factor is biotin and/or nicotinic acid.
5. The biostabilizer for inhibiting spoilage of metal processing fluids according to claim 1, wherein: the balancing agent is one or more than two of sodium glutamate, sodium alginate, sucrose, corn flour, cake flour, bran, turf, lactose, galactose and arabinose;
preferably, the balancing agent is sodium glutamate or/and galactose.
6. The biostabilizer for inhibiting spoilage of metal processing fluids according to claim 1, wherein: the protozoa is one or more than two of trichoderma, streptomyces, chaetomium, pseudomonas and bacillus;
preferably, the balancing agent is at least one of trichoderma and pseudomonas.
7. A method of preparing a biostabilizer for inhibiting spoilage of metal processing fluids according to any one of claims 1 to 6, wherein: the method comprises the following steps:
(1) stirring and dissolving organic carbon and organic nitrogen in a formula amount at 50-70 ℃ until a clear and transparent oily substance A is obtained;
(2) stirring the water and the balancing agent in the formula amount at 30-35 ℃ until the water and the balancing agent are completely dissolved to obtain clear transparent liquid B;
(3) when A is cooled to 30-35 ℃, B is added into A and stirred until the B is completely dissolved to form clear transparent liquid C;
(4) adding the growth factor and the protobacteria powder thalli with the formula amount into the C, and continuously stirring until the mixture is uniform to obtain the biological stabilizer.
8. The method of claim 7, wherein the biostabilizer is selected from the group consisting of: in the step (4), the preparation method of the protobacteria powder thallus comprises the following steps:
s1: extracting activated protobacteria, inoculating the extracted activated protobacteria into a proper amount of a protobacteria liquid culture medium, and performing amplification culture twice to obtain a bacterial liquid;
s2: collecting bacteria liquid, centrifuging, removing supernatant, washing the precipitated bacteria with 0.9% sterile normal saline, centrifuging, and removing supernatant to obtain precipitate, i.e. protobacteria bacteria;
s3: and fully drying the thallus of the protobacteria to obtain the powder thallus of the protobacteria.
9. The method of claim 8, wherein the biostabilizer is selected from the group consisting of: in step S1, the condition of two times of amplification culture is that the culture is carried out for 1 to 5 days at the temperature of 20 to 40 ℃;
and/or, in step S1, the number of colonies of the original bacteria in the activated protobacteria is 107-109The dosage volume ratio of the activated protobacteria to the liquid culture medium is 1: 100.
And/or in step S3, the drying temperature is 20-40 ℃.
10. Use of a biostabilizer according to any one of claims 1 to 6 for inhibiting spoilage of a metal working fluid in a metal working fluid and/or machining.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911164013.1A CN111019748A (en) | 2019-11-25 | 2019-11-25 | Biological stabilizer for inhibiting metal processing liquid from putrefaction and preparation method and application thereof |
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Cited By (2)
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| CN111662763A (en) * | 2020-07-17 | 2020-09-15 | 清华大学天津高端装备研究院 | Biological bacteriostatic agent of emulsified water-based metal working fluid and preparation method thereof |
| CN112852521A (en) * | 2021-01-11 | 2021-05-28 | 陈雪莲 | Method for preventing metal working fluid from smelling by adopting biological balance technology |
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Cited By (3)
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|---|---|---|---|---|
| CN111662763A (en) * | 2020-07-17 | 2020-09-15 | 清华大学天津高端装备研究院 | Biological bacteriostatic agent of emulsified water-based metal working fluid and preparation method thereof |
| CN111662763B (en) * | 2020-07-17 | 2021-08-13 | 清华大学天津高端装备研究院 | Biological bacteriostatic agent of emulsified water-based metal working fluid and preparation method thereof |
| CN112852521A (en) * | 2021-01-11 | 2021-05-28 | 陈雪莲 | Method for preventing metal working fluid from smelling by adopting biological balance technology |
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