CN111684057B - Bacterial cellulose high-yield bacterial strain and preparation method of cellulose tablet by using bacterial cellulose high-yield bacterial strain - Google Patents

Abstract

The invention provides a bacterial cellulose high-yield strain (Komagataeibacter rhaeticus) KOSS15, a composition for preparing cellulose sheets comprising the strain or the culture, and a method for preparing cellulose sheets comprising the step of culturing the strain. The cellulose sheet produced by the bacterial cellulose highly-producing strain KOSS15 of the present invention exhibits high tensile strength and burst strength as compared with those of cellulose sheets of Acetobacter xylinum, which have been known to have the highest productivity of cellulose, and can reduce the sugar content required for producing cellulose sheets of the same weight by 5 to 6 times.

Description

Bacterial cellulose high-yield bacterial strain and preparation method of cellulose tablet by using bacterial cellulose high-yield bacterial strain

Technical Field

The present patent application claims priority from korean patent application No. 10-2018-011099, filed in the korean patent office on month 17 of 2018, the disclosure of which is incorporated herein by reference.

The invention relates to a novel bacterial cellulose high-yield bacterial strain and a preparation method of cellulose tablets using the bacterial cellulose high-yield bacterial strain.

Background

A sheet type mask for use in the cosmetic field may generally have three types including: a cellulose mask (i) for forming a mask-shaped support by immersing a non-woven fabric prepared from plant cellulose fibers such as cotton and pulp or synthetic fibers such as nylon and polyester into a pale water body of a cosmetic emulsion; hydrogel mask (ii) prepared from natural polysaccharide such as natural agar (agar), carrageenan, locust bean gum; and a bacterial cellulose mask (iii) which is prepared by culturing microorganisms, and which is formed into a mask-shaped support by immersing bacterial cellulose (bacterial cellulose, biocellulose) in a body of water for cosmetics.

The sheet type mask for the cosmetic field is applied by hands, and only the sheet manufactured in the form of a face is required to be applied, and has the greatest advantage of moisturizing and cleaning effects, so that the mask is attracting attention in the cosmetic market.

Recently, cellulose (also called bacterial cellulose) produced by microbial culture has been increasingly utilized as a raw material for a burn healing agent for pharmaceuticals or a cosmetic mask. Cellulose is the most abundant biomass resource in nature, and particularly, cellulose produced from acetic acid bacteria is attracting attention not only as an additive for foods but also as a new industrial material with high added value. Moreover, cellulose produced by bacteria has excellent physicochemical properties unlike cellulose produced by plant bodies.

Bacterial cellulose-producing strains include Acetobacter sp, agrobacterium sp, rhizobium sp, pseudomonas sp and Sarcina sp, and particularly Acetobacter sp, acetobacter Pasteum A.pastoris A.hansenii and Acetobacter hansenii A.hansenii are well known among Acetobacter strains, and the microorganism having the highest cellulose production yield is Acetobacter xylinum A.hansenii (Korean society of industrial scale KIC News,2013 16 (4) 37-45).

As is well known, microorganisms producing such bacterial cellulose are relatively inefficient in production, and thus, there is a need to develop novel microorganisms having relatively high cellulose production efficiency.

Disclosure of Invention

Technical problem

The inventors of the present invention have developed a method for producing bacterial cellulose which is excellent in quality and can improve productivity, and studied the quality and productivity changes of bacterial cellulose by digging a novel strain and sugar source of a culture solution. As a result, the present invention has been completed by identifying a novel bacterial cellulose high-yielding strain (Komagataeibacter rhaeticus) KOSS15, and it has been found that the above strain uses honey as a sugar source, and thus has a high cellulose productivity and the produced bacterial cellulose has excellent quality.

Accordingly, an object of the present invention is to provide a bacterial cellulose highly productive bacterial KOSS15 strain.

It is still another object of the present invention to provide a composition for producing bacterial cellulose tablets.

It is another object of the present invention to provide a method for preparing bacterial cellulose.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, the scope of the invention as claimed, and the accompanying drawings.

Technical proposal

According to an embodiment of the present invention, there is provided bacterial cellulose high-yielding strain (Komagataeibacter rhaeticus) KOSS15 having accession No. KCCM12270P.

The inventors of the present invention have developed a method for producing bacterial cellulose which is excellent in quality and can improve productivity, and studied the quality and productivity changes of bacterial cellulose by the excavation of novel strains and the sugar source of the culture solution. As a result, the present invention has been completed by identifying a novel bacterial cellulose high-yielding strain (Komagataeibacter rhaeticus) KOSS15, and it has been found that the above strain uses honey as a sugar source, and thus has a high cellulose productivity and the produced bacterial cellulose has excellent quality.

According to an embodiment of the present invention, the bacterial cellulose-producing high-yield strain KOSS15 is derived from black tea fungus (kombucha).

The black tea fungus is one of organic acid-producing fungus (lactobacillus) produced by symbiotic bacteria and yeast with hypha such as silk as parent, and has various names such as Kang Pucha, black tea mushroom yeast, red mushroom, and long life mushroom. Black tea fungus is a symbiont of yeasts such as bacteria and Saccharomyces cerevisiae, zygosaccharomyces, candida, schizosaccharomyces pombe, and Saccharomyces (Saccharomyces), and the produced alcohols are fermented into acetic acid and other acids by yeasts such as Acetobacter (A. Xylinum) to increase acidity.

According to an example of the present invention, the bacterial cellulose high-yielding strain KOSS15 described above has cellulose productivity.

The term "cellulose" in the present specification means that beta-D-glucose forms a polymer through glycosidic bond, and has the chemical formula (C ₆ H ₁₀ O ₅ ) And n, the unit body is polysaccharide of cellobiose (cellobiose).

According to still another embodiment of the present invention, the bacterial cellulose high-yielding strain KOSS15 described above produces cellulose from honey as a sugar source.

The term "honey" in the present specification is a substance having viscosity collected from the glands of plants by bees, and means a substance containing various vitamins, proteins, mineral components in addition to high contents of glucose and fructose.

According to other embodiments of the present invention, the present invention relates to a composition for preparing cellulose sheets comprising bacterial cellulose high-yielding strain KOSS15 having accession No. KCCM12270P or a culture of the above strain.

The term "culture" of the present invention means a culture medium comprising a strain which is obtained by culturing the above strain in a medium capable of providing nutrients for a period of time in order to allow the bacterial cellulose-producing high-yield strain KOSS15 of the present invention to grow and survive in vitro, a metabolite thereof, the balance of nutrients, and the like. The bacterial cellulose-producing high-yield strain KOSS15 described above is used as a strain having cellulose-producing ability, and therefore, the bacterial cellulose-producing high-yield strain KOSS15 described above or a culture thereof can be used in a composition for producing cellulose sheets from a sugar source.

According to another embodiment of the present invention, the present invention relates to a method for producing a cellulose sheet comprising the step of culturing a bacterial cellulose high-yielding strain KOSS15 having accession No. KCCM12270P.

The bacterial cellulose high-yielding strain KOSS15 can be used for producing cellulose by using honey, fructose, glucose or a combination thereof.

According to an example of the present invention, the bacterial cellulose-producing KOSS15 strain is cultured in a medium comprising (a) honey or (b) a sugar source of glucose and fructose and proline.

The culture medium may comprise 0.1-2.0 volume percent, 0.2-2.0 volume percent, 0.3-2.0 volume percent, 0.4-2.0 volume percent, 0.5-2.0 volume percent, 0.6-2.0 volume percent, 0.7-2.0 volume percent, 0.8-2.0 volume percent, 0.9-2.0 volume percent, 0.1-1.9 volume percent, 0.1-1.8 volume percent, 0.1-1.7 volume percent, 0.1-1.6 volume percent, 0.1-1.5 volume percent, 0.1-1.4 volume percent, 0.1-1.3 volume percent, 0.1-1.2 volume percent, 0.1-1.1.0 volume percent, 0.2-1.8 volume percent, 0.2-1.6 volume percent, 0.2-1.4 volume percent or 0.2-1.2 volume percent of honey.

The medium may comprise 0.1 to 2.0 wt%, 0.2 to 2.0 wt%, 0.3 to 2.0 wt%, 0.4 to 2.0 wt%, 0.5 to 2.0 wt%, 0.6 to 2.0 wt%, 0.7 to 2.0 wt%, 0.8 to 2.0 wt%, 0.9 to 2.0 wt%, 0.1 to 1.9 wt%, 0.1 to 1.8 wt%, 0.1 to 1.7 wt%, 0.1 to 1.6 wt%, 0.1 to 1.5 wt%, 0.1 to 1.4 wt%, 0.1 to 1.3 wt%, 0.1 to 1.2 wt%, 0.1 to 1.1 wt%, 0.2 to 1.8 wt%, 0.2 to 1.6 wt%, 0.2 to 1.4 wt%, or 0.2 to 1.2 wt% fructose.

Although the above-mentioned medium may contain 0.01 to 1.0 wt%, 0.02 to 1.0 wt%, 0.03 to 1.0 wt%, 0.04 to 1.0 wt%, 0.05 to 1.0 wt%, 0.06 to 1.0 wt%, 0.07 to 1.0 wt%, 0.08 to 1.0 wt%, 0.01 to 0.8 wt%, 0.01 to 0.6 wt%, 0.01 to 0.4 wt%, 0.01 to 0.2 wt%, 0.02 to 0.6 wt%, 0.03 to 0.6 wt%, 0.04 to 0.6 wt%, 0.05 to 0.4 wt%, 0.06 to 0.3 wt%, 0.04 to 0.2 wt%, or 0.05 to 0.15 wt% of proline, it is not limited thereto.

The above fructose, glucose and proline are main saccharides and amino acids constituting Lac Regis Apis, and when bacterial cellulose high-yielding strain KOSS15 is cultured, bacterial cellulose can be prepared by adding main saccharides and amino acids constituting Lac Regis Apis instead of Mel.

The composition for producing cellulose sheet of the present invention may further comprise a carbon source, a nitrogen source and a phosphorus source (phosphorus) for culturing the bacterial cellulose highly productive KOSS15 strain.

The carbon source includes at least one carbon source selected from the group consisting of ethanol, glucose, sucrose, fructose, galactose, soluble starch, inositol, glycerol, xylose, dextrose, lactose, dextrin, ribitol, mannitol, mannose, maltose and raffinose, but is not limited thereto.

The nitrogen source comprises at least one organic nitrogen source selected from the group consisting of yeast extract, peptone, soybean protein, casamino acid, tryptone and malt extract; selected from ammonium chloride (NH) ₄ Cl), ammonium carbamate ((COONH) ₄ ) ₂ ) Monoammonium phosphate (NH) ₄ H ₂ PO ₄ ) Diammonium phosphate ((NH) ₄ ) ₂ HPO ₄ ) Ammonium Nitrate (NH) ₄ NO ₃ ) Sodium nitrate (NaNO) ₃ ) Ammonium sulfate ((NH) ₄ ) ₂ SO ₄ ) One or more inorganic nitrogen sources in the group consisting of, but not limited to, these.

The phosphorus source includes one or more phosphorus sources selected from the group consisting of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate, but is not limited thereto.

The composition for producing cellulose sheet may further contain magnesium sulfate (MgSO ₄ ) Calcium chloride (CaCl) ₂ ) Potassium chloride (KCl), barium chloride (BaCl) ₂ ) Sulfur and sulfurLithium acid (Li) ₂ SO ₄ ) Anhydrous manganese sulfate (MnSO), manganese sulfate tetrahydrate (MnSO) ₄ ) Zinc sulfate (ZnSO) ₄ ) Ferrous sulfate (FeSO) ₄ ) Sodium molybdate (Na) ₂ MoO ₄ ) Monopotassium phosphate (KH) ₂ PO ₄ ) Ferric chloride (FeCl) ₃ ) However, the present invention is not limited thereto.

According to other embodiments of the present invention, the present invention relates to a method for producing a bacterial cellulose sheet using bacterial cellulose high-yielding strain KOSS15 having accession No. KCCM12270P, comprising:

a preculture step (a) of culturing the strain in a preculture medium containing a sugar source and cellulase with stirring;

a main culture step (b) of adding the preculture solution of the above step to a main culture medium containing a sugar source and proline, and culturing under stirring; and

and (c) a plate-transformation culturing step of adding the main culture solution of the above step to a main culture medium containing a sugar source and proline, thereby performing stationary culture.

The preparation method of the bacterial cellulose sheet of the present invention is described in terms of the respective steps.

Step (a): pre-cultivation step

First, a bacterial cellulose high-yielding strain KOSS15 having accession No. KCCM12270P was cultured with stirring in a preculture medium containing a sugar source and cellulase.

According to an embodiment of the invention, the pre-culture medium comprises a sugar source.

According to an embodiment of the present invention, the sugar source is 0.1 to 5 volume percent (v/v) of (a) honey or 0.1 to 5 weight percent (w/v) of (b) glucose and fructose.

According to a specific example of the present invention, the culture medium of the present invention comprises honey at a concentration of 0.1 to 5 volume percent, 0.1 to 4 volume percent, 0.1 to 3 volume percent, 0.1 to 2.5 volume percent, 0.1 to 2 volume percent, 0.1 to 1.5 volume percent, or 0.1 to 1 volume percent.

According to other examples, the culture medium of the present invention comprises 0.1 to 3.0 volume percent, 0.2 to 3.0 volume percent, 0.3 to 3.0 volume percent, 0.4 to 3.0 volume percent, 0.5 to 3.0 volume percent, 0.6 to 3.0 volume percent, 0.7 to 3.0 volume percent, 0.8 to 3.0 volume percent, 0.9 to 3.0 volume percent, 1 to 3.0 volume percent, 0.1 to 2.0 volume percent, 0.2 to 2.0 volume percent, 0.3 to 2.0 volume percent, 0.4 to 2.0 volume percent, 0.5 to 2.0 volume percent, 0.6 to 2.0 volume percent, 0.7 to 2.0 volume percent, 0.8 to 2.0 volume percent, 0.9 to 2.0 volume percent, 0.1 to 1.9 volume percent, 0.1 to 1.0 volume percent, 0.1 to 2.0 volume percent, 0.1 to 1.4 volume percent, 0.1 to 1.0 volume percent, 0 volume percent, 0.1 to 1.1 volume percent or 2.1 to 2.0 volume percent.

According to other embodiments of the present invention, the culture medium composition of the present invention comprises glucose and fructose at a concentration of 0.1 to 5 weight percent, 0.1 to 4 weight percent, 0.1 to 3 weight percent, 0.1 to 2.5 weight percent, 0.1 to 2 weight percent, 0.1 to 1.5 weight percent, or 0.1 to 1 weight percent in total.

According to another example of the present invention, the medium of the present invention comprises 0.1 to 3.0 weight percent, 0.2 to 3.0 weight percent, 0.3 to 3.0 weight percent, 0.4 to 3.0 weight percent, 0.5 to 3.0 weight percent, 0.6 to 3.0 weight percent, 0.7 to 3.0 weight percent, 0.8 to 3.0 weight percent, 0.9 to 3.0 weight percent, 1 to 3.0 weight percent, 0.1 to 2.0 weight percent, 0.2 to 2.0 weight percent, 0.3 to 2.0 weight percent, 0.4 to 2.0 weight percent, 0.5 to 2.0 weight percent, 0.6 to 2.0 weight percent, 0.7 to 2.0 weight percent, 0.8 to 2.0 weight percent, 0.9 to 2.0 weight percent, 0.1 to 1.9 weight percent, 0.1 to 1.0 weight percent, 0.2.2.0 weight percent, 0.2 to 2.0 weight percent, 0.3 to 2.0 weight percent, 0.1 to 2.0 weight percent, 0.5 to 2.0 weight percent, 0.0 weight percent, 0.4 to 2.0 weight percent, 0.0 weight percent, 0.5 to 2.0 weight percent, 0 weight percent, 0.1 to 1 weight percent, 1.4 weight percent, 1 to 2.0 weight percent, 1 or 1 to 2.1 weight percent of fructose.

According to other examples of the invention, at 2:3 comprises the glucose and fructose in a weight ratio. The ratio of glucose and fructose contained in royal jelly can lead to the above 2: 3.

According to an embodiment of the present invention, the pre-culture medium comprises 0.1 to 10.0 wt% of yeast extract, 0.002 to 0.2 wt% of magnesium sulfate (MgSO) ₄ ) 0.002 to 0.2 weight percent calcium chloride (CaCl) ₂ ) 0.2 to 20.0 weight percent ethanol and 0.0005 to 0.05 weight percent cellulase or combinations thereof.

The term "preculture" in the present specification means a step of culturing a stock cellulose producing strain to a smaller scale (for example, 100L or less) for the purpose of activating the cellulose producing strain or increasing bacteria.

The pre-culture medium may comprise 0.1 to 10.0 wt%, 0.5 to 10.0 wt%, 0.7 to 10.0 wt%, 0.9 to 10.0 wt%, 0.1 to 8.0 wt%, 0.1 to 6.0 wt%, 0.1 to 4.0 wt%, 0.1 to 2.0 wt%, 0.5 to 10.0 wt%, 0.5 to 8.0 wt%, 0.5 to 6.0 wt%, 0.5 to 4.0 wt%, 0.5 to 2.0 wt%, 0.9 to 10.0 wt%, 0.9 to 8.0 wt%, 0.9 to 6.0 wt%, 0.9 to 4.0 wt%, 0.9 to 2.0 wt%, or 0.9 to 1.5 wt% of the yeast extract.

The preculture medium may comprise 0.002-0.2 wt%, 0.005-0.2 wt%, 0.008-0.2 wt%, 0.01-0.2 wt%, 0.013-0.2 wt%, 0.015-0.2 wt%, 0.01-0.15 wt%, 0.01-0.1 wt%, 0.01-0.05 wt%, 0.01-0.03 wt% or 0.015-0.03 wt% of magnesium sulfate (MgSO) ₄ )。

The preculture medium may comprise 0.002-0.2 wt%, 0.005-0.2 wt%, 0.008-0.2 wt%, 0.01-0.2 wt%, 0.013-0.2 wt%, 0.015-0.2 wt%, 0.01-0.15 wt%, 0.01-0.1 wt%, 0.01-0.05 wt%, 0.01-0.03 wt% or 0.015-0.03 wt% of calcium chloride (CaCl) ₂ )。

The pre-culture medium may comprise 0.2 to 20.0 volume percent, 0.5 to 20.0 volume percent, 1.0 to 20.0 volume percent, 1.5 to 20.0 volume percent, 1.0 to 17.0 volume percent, 1.0 to 14.0 volume percent, 1.0 to 11.0 volume percent, 1.0 to 8.0 volume percent, 1.0 to 5.0 volume percent, or 1.0 to 3.0 volume percent ethanol.

The pre-culture medium may comprise 0.0005 to 0.05 wt%, 0.001 to 0.05 wt%, 0.003 to 0.05 wt%, 0.001 to 0.03 wt%, 0.001 to 0.02 wt%, or 0.001 to 0.01 wt% cellulase.

According to an embodiment of the present invention, the above-mentioned preculture step is performed at 100 to 200rpm for 24 to 72 hours with stirring.

The above-mentioned preculture step may be performed for 30 hours to 72 hours, 36 hours to 72 hours, 42 hours to 72 hours, 36 hours to 66 hours, 36 hours to 60 hours, 36 hours to 54 hours, 42 hours to 66 hours, 42 hours to 60 hours, 42 hours to 54 hours, or 42 hours to 52 hours of agitation culture.

According to other examples of the present invention, the above-mentioned preculture step is performed at 160rpm for 48 hours with stirring.

Step (b): main culture step

Then, the preculture solution of the above step was added to the main medium containing the saccharide source and proline to stir the culture.

The term "main culture" in the present specification means a step of culturing bacterial cellulose-producing strains on a large scale (for example, 500L or more and 50000L or less) by a culture solution of "preculture" until the preceding step of the "sheet transformation culture" step of actually producing bacterial cellulose sheets.

The main culture medium comprises 0.01-1.0 wt% of proline, 0.002-0.2 wt% of magnesium sulfate (MgSO) ₄ ) 0.002 to 0.2 weight percent calcium chloride (CaCl) ₂ ) 0.01 to 1.0 weight percent sodium acetate and 0.02 to 2.0 weight percent acetic acid or combinations thereof.

The main culture medium may comprise 0.01-1.0 wt%, 0.02-1.0 wt%, 0.03-1.0 wt%, 0.04-1.0 wt%, 0.05-1.0 wt%, 0.06-1.0 wt%, 0.07-1.0 wt%, 0.08-1.0 wt%, 0.01-0.8 wt%, 0.01-0.6 wt%, 0.01-0.4 wt%, 0.01-0.2 wt%, 0.02-0.6 wt%, 0.03-0.6 wt%, 0.04-0.6 wt%, 0.05-0.4 wt%, 0.06-0.3 wt%, 0.04-0.2 wt% or 0.05-0.15 wt% of proline.

The main culture medium may comprise 0.002-0.2 wt%, 0.005-0.2 wt%, 0.008-0.2 wt%, 0.01-0.2 wt%, 0.013-0.2 wt%, 0.015-0.2 wt%, 0.01-0.15 wt%, 0.01-0.1 wt%, 0.01-0.05 wt%, 0.01-0.03 wt% or 0.015-0.03 wt% of magnesium sulfate (MgSO) ₄ )。

The main culture medium may comprise 0.002-0.2 wt%, 0.005-0.2 wt%, 0.008-0.2 wt%, 0.01-0.2 wt%, 0.013-0.2 wt%, 0.015-0.2 wt%, 0.01-0.15 wt%, 0.01-0.1 wt%, 0.01-0.05 wt%, 0.01-0.03 wt% or 0.015-0.03 wt% of calcium chloride (CaCl) ₂ )。

The main culture medium may comprise 0.01-1.0 wt%, 0.02-1.0 wt%, 0.03-1.0 wt%, 0.04-1.0 wt%, 0.05-1.0 wt%, 0.06-1.0 wt%, 0.07-1.0 wt%, 0.08-1.0 wt%, 0.01-0.8 wt%, 0.01-0.6 wt%, 0.01-0.4 wt%, 0.01-0.2 wt%, 0.02-0.6 wt%, 0.03-0.6 wt%, 0.04-0.6 wt%, 0.05-0.4 wt%, 0.06-0.3 wt%, 0.04-0.2 wt% or 0.05-0.15 wt% sodium acetate.

The main culture medium may contain 0.02-2.0 wt%, 0.05-2.0 wt%, 0.1-2.0 wt%, 0.15-2.0 wt%, 0.1-1.5 wt%, 0.1-1.0 wt% or 0.1-0.5 wt% of acetic acid.

Step (c): sheet transformation culture step

Finally, the main culture medium containing the saccharide source and proline is added to the main culture medium in the above steps to perform stationary culture, thereby performing plate-transfer culture.

The term "sheet transformation culture" in the present specification means a step of allowing a bacterial cellulose producing strain to produce bacterial cellulose sheets by stationary culturing a main culture liquid containing the bacterial cellulose producing strain.

The above medium has the same composition as the main medium of step (b).

The method for producing a bacterial cellulose sheet according to the present invention is similar to the bacterial cellulose high-producing strain KOSS15 described above, a culture of the strain, and a composition for producing a cellulose sheet, and in order to avoid complicating the present description due to the same matters, descriptions thereof are omitted below.

ADVANTAGEOUS EFFECTS OF INVENTION

The invention provides a bacterial cellulose high-yield strain (Komagataeibacter rhaeticus) KOSS15, a composition for preparing cellulose sheets comprising the strain or the culture, and a method for preparing cellulose sheets comprising the step of culturing the strain. The cellulose sheet produced by the bacterial cellulose highly-producing strain KOSS15 of the present invention exhibits high tensile strength and burst strength as compared with those of cellulose sheets of Acetobacter xylinum, which have been known to have the highest productivity of cellulose, and can reduce the sugar content required for producing cellulose sheets of the same weight by 5 to 6 times.

Drawings

FIG. 1 is a diagram showing a phylogenetic tree of bacterial cellulose high-yielding strain KOSS 15.

Fig. 2 is a diagram showing a cellulose production process using bacterial cellulose high-yielding strain KOSS 15.

Fig. 3 is a schematic diagram showing a preparation process of cellulose sheets of bacterial cellulose high-yielding strain KOSS15 by subculturing.

Fig. 4 is a diagram showing a cellulose sheet produced by culturing bacterial cellulose high-yielding strain KOSS15 strain.

FIG. 5 is a diagram showing a cellulose sheet of bacterial cellulose highly productive bacteria KOSS15 and Acetobacter xylinum IFO13693 produced by cellulase treatment.

Fig. 6 is a graph showing cellulose sheets of bacterial cellulose high-yielding strain KOSS15 prepared according to the concentration of honey in a medium.

Fig. 7 is a view showing a cellulose sheet of bacterial cellulose high-yielding strain KOSS15 prepared according to the kind of honey in a medium.

FIG. 8 shows the results of dielectric analysis of cellulose production genomes of bacterial cellulose high yielding strain KOSS15 and Acetobacter xylinum IFO13693.

Detailed Description

Hereinafter, the present invention will be described in more detail by way of examples. These examples are merely to more specifically illustrate the present invention, and it will be apparent to those skilled in the art that the scope of the present invention according to the gist of the present invention is not limited by these examples.

Examples

In the entire specification, when "%" used for expressing the concentration of a specific substance is not mentioned alone, solid/solid is (weight/weight)%, solid/liquid is (weight/volume)%, and liquid/liquid is (volume/volume)%.

1. Strain screening

3 bags of brown rice green tea were soaked in 1L of water, 30% (w/v) white sugar was added thereto, and 500ml of each was dispensed into a flask, and then sterilized and cooled at 121℃for 30 minutes. The obtained 5 kinds of black tea fungus solutions (Vietnam black tea mushroom, zhiyishan green tea mushroom, baocheng green tea mushroom, yunnan black tea mushroom in China, and Tibet black tea mushroom in China) were inoculated at 10% (v/v), and were subjected to stationary culture in an incubator at 28 ℃. After 2 weeks of culture, it was confirmed that cellulose was formed, 2% glucose, 1% yeast extract, 0.02% magnesium sulfate, 0.02% calcium chloride and 1.8% agar were added after the collection of bacteria in one experimental zone. After the pH of the medium was adjusted to 4.0 by acetic acid, an agar medium was prepared. One liquid (Zhiyishan green tea black tea mushroom) selected from 5 liquids of the agar medium was smeared at 0.1ml and cultured at 28℃for 7 days. After separating colonies having the same shape as the bacteria into 6 groups, 60ml of a medium solution containing 2% glucose, 1% yeast extract, 0.02% magnesium sulfate and 0.02% calcium chloride was sterilized. The sterilized medium was then dispensed at 10ml each and 6 colonies were inoculated here each.

After 3 days of culture at 28℃an acetic acid preculture medium was prepared in 40ml, and 4ml of the culture broth was placed in the acetic acid preculture medium for 3 days, and after 4 days of stationary culture at 28℃a morphology of cellulose was observed. Finally, the label presents a colony with good cellulose morphology, thereby preparing a stock bacterium. The most well formed colony in 6 experimental areas was designated as KOSS15, and patent deposit number KCCM12270P was granted from the korean microorganism preservation center.

2. Strain culture

(1) Culture of Acetobacter (Acetobacter oxydans) IFO13693 strain

1) Pre-culture (1 passage)

As a control group for comparison of the cellulose bioconversion efficiency of bacterial cellulose-producing strain KOSS15, acetobacter xylinum (Acetobactor xylinum) IFO13693 was used. The above acetobacter xylinum is a representative cellulose producing strain.

Acetobacter xylinum IFO13693 was treated with a solution comprising 2% glucose, 1.0% yeast extract, 0.02% magnesium sulfate (MgSO ₄ ) 0.02% calcium chloride (CaCl) ₂ ) And 2.0X10% ethanol in the medium ⁷ CFU/ml was inoculated and precultured in flasks (flash) at 160rpm for 48 hours at 30 ℃.

2) Main culture (2 passage)

Subsequently, the slurry (residue) produced from the preculture was filtered through a 80mesh sieve and inoculated with 10% of the main culture broth. By adding 3% glucose, 0.5% sodium glutamate (MSG, monosodium glutamate), 0.02% magnesium sulfate (MgSO ₄ ) 0.02% calcium chloride (CaCl) ₂ ) The main culture medium was prepared with 0.1% sodium acetate and 0.2% acetic acid. The main culture was a static culture at 0rpm and 0.3vvm (application volume/medium volume/minute) for 48 hours.

3) Sheet transformation culture (Main culture liquid mixing and tray dispensing) and washing

Finally, the main culture of Acetobacter xylinum IFO13693 was inoculated and mixed in an amount of 5 to 10% based on the medium, and the mixture was dispensed on a tray, and the mixture was left to stand at 0rpm and 0.3vvm for 3 days to induce conversion of cellulose sheets (FIGS. 2 and 3). After the completion of the culture, the prepared cellulose sheet was subjected to dehydration post-treatment with 0.1% sodium hydroxide (NaOH), whereby washing was performed by removing the excess strain. Subsequently, 5% sodium percarbonate was treated and washed by removing proteins and impurities to obtain a sheet (fig. 4).

(2) Culture of bacterial cellulose-producing high-yield strain KOSS15 (KCCM 12270P)

1) Pre-culture (1 passage)

The bacterial cellulose high-yielding strain KOSS15 is prepared from sugar source (0.6% fructose and 0.4% glucose or 1% Mel), 1.0% yeast extract, 0.02% magnesium sulfate (MgSO) ₄ ) 0.02% calcium chloride (CaCl) ₂ ) 1.0X10% in medium of 2% ethanol and 0.005% cellulase ⁸ CFU/ml was inoculated and precultured in flasks (flash) at a temperature of 30℃and at 80-160 rpm for 48 hours. In the case of preculture, the cellulase is added to prevent the formation of a pellicle (pellicle), and if cellulase is added to the preculture medium of Acetobacter xylinum IFO13693 of the above (1), a defective sheet is generated, and therefore, it is not suitable (FIG. 5).

2) Main culture (2 passage)

Subsequently, 10% of the preculture was inoculated with respect to the main medium. By adding sugar source (0.6% fructose and 0.4% glucose or 1% Mel), 0.1% proline, 0.02% magnesium sulfate (MgSO) ₄ ) 0.02% calcium chloride (CaCl) ₂ ) The main culture medium was prepared with 0.1% sodium acetate and 0.2% acetic acid. The main culture was performed at 80rpm and 0.3vvm for 48 hours under stirring.

3) Sheet transformation culture (Main culture liquid mixing and tray dispensing) and washing

Finally, the main culture of Acetobacter xylinum IFO13693 was inoculated and mixed in an amount of 5 to 10% based on the medium, and the mixture was dispensed on a tray, and the mixture was left to stand at 0rpm and 0.3vvm for 2 days to induce conversion of cellulose sheets (FIGS. 2 and 3). After the end of the incubation, 0.1% sodium hydroxide (NaOH) was treated overnight (overlapping), whereby post-dehydration washing was performed by removing excess strain. Subsequently, 5% sodium percarbonate was treated and washed by removing proteins and impurities to obtain a sheet (fig. 4). Unlike the sheet produced by acetobacter xylinum IFO13693, the cellulose sheet produced by the above bacterial cellulose highly productive bacteria KOSS15 has a characteristic that the sheet becomes gradually hard and has excellent strength when immersed in an aqueous solution of sodium hydroxide (NaOH) before the sheet is dehydrated.

3. Preparation of cellulose tablets

3.1. Drying weight of cellulose tablet according to the type and content of sugar source in the culture medium

The washed cellulose sheet was cut into 18.15X10.15 cm pieces after being laid flat, and the weight was measured. After the measurement, drying was performed in a drying oven at a temperature of 95℃for 1 hour, and the weight thereof was measured, and the weights before and after the drying were calculated.

The content of the sugar source in the medium in the following table 1 means the content of the sugar source in the medium when the cellulose sheet is transformed and cultured.

TABLE 1

From this, it was confirmed that when tablets of the same weight were prepared, bacterial cellulose high-yielding strain KOSS15 was able to reduce the content of the sugar source in the medium by about 6 times as compared with Acetobacter xylinum IFO13693. And, when using 3:2, the same effect can be exhibited when the sugar source containing fructose and glucose is substituted for honey.

3.2. Composition of cellulose tablet according to sugar source and content in culture medium

The content of the constituent sugar of the cellulose sheet was confirmed.

TABLE 2

3.3. Physical properties of cellulose sheet according to sugar sources in the medium

The tensile strength of the sheet was measured by a tester applying the constant-speed elongation method (20 mm/min) (KS M ISO 1924-2).

The wet burst strength of the sheet was measured by increasing the fluid pressure (KS M ISO 2758).

TABLE 3 Table 3

3.4. Physical property comparison of constituent sugar and cellulose tablets according to concentration of Mel in culture medium

Cellulose sheets were produced in the same manner as in example 2 above, except that the types and contents of sugar sources of the medium used in the sheet transformation culturing step were as shown in Table 4, and 0.3% honey, 0.5% honey and 1.0% honey were as shown in Table 4 below. The type and content of sugar sources in the medium were measured based on the honey concentrations before and after the culture, and the physical properties of the cellulose tablets prepared according to the honey concentrations contained in the medium were compared (Table 4).

TABLE 4 Table 4

As shown in table 4, from the concentration of honey contained in the culture medium, it was confirmed that the proportion of the constituent sugar and the dry weight of the tablets increased.

3.5. Comparison of dry weights of sugar and cellulose tablets according to the composition of the honey species in the medium

To confirm the productivity of cellulose sheets according to the kind of honey, cellulose sheets were produced by the same method as in example 2 above except that 0.5% of cultivated honey, 0.5% of locust honey, and 0.55% of wild flower honey (miscellaneous honey) were used as sugar sources of the medium used in the sheet transformation cultivation step, as shown in table 5 below. The type and content of sugar source in the medium were measured based on the types of honey before and after the culture, and the dry weights of cellulose sheets prepared for each type of honey contained in the medium were compared (Table 4).

The results are shown in Table 5.

TABLE 5

As shown in table 5, although it can be confirmed that the drying weight (production efficiency) for cellulose production is excellent according to the bee species, it can be confirmed that the locust honey and the wild honey (miscellaneous honey) are more excellent and the wild honey (miscellaneous honey) is most excellent (fig. 6 and 7).

4. Cellulose production Gene analysis of bacterial cellulose high-yielding Strain KOSS15 (K.rhaeticus KOSS 15)

4.1. Research of novel cellulose-producing genes

IFO13693 and KOSS15 were sequenced by one of the next generation of base sequence analyses (NGS, next generation sequencing), i.e., pacbio sequence analysis. In the case of the IFO13693 strain, it was confirmed to have chromosome 3539432bp. In the case of KOSS15 strain, it was confirmed that there were 3275555bp and 3 plasmids on the chromosome. Based on the new generation of base sequence analysis (NGS, next generation sequencing), the production genes of the biological cellulose were confirmed in the two production strains, respectively (fig. 8).

TABLE 6

While specific portions of the invention have been described in detail above, it will be appreciated by those skilled in the art that such specific techniques are merely preferred examples, and that the scope of the invention is not so limited.

Deposit number

Preservation agency name: korean microorganism preservation center (foreign)

Preservation number: KCCM12270P

Preservation date: 20180530

Korean microorganism preservation center

First city Siemens gate area Hongji inner 2 street 45 elm forest ∈10->

<110> Aikebailangde Co., ltd

<120> bacterial cellulose high-yielding strain and method for producing cellulose sheet using the same

<130> 1017-047.20P8

<150> KR 10-2018-0111099

<151> 2018-09-17

<160> 38

<170> KoPatentIn 3.0

<210> 1

<211> 2001

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1824 (BcsA I)

<400> 1

gtgctttcgg cgctggtgtc cctgcgttat ctgacgtggc ggctgaccga aacactggat 60

ttcgatacat ggctccaggg tacattgggg gtcacgctgc ttctggcgga actgtacgcg 120

ctgtacatgc tgttcctcag ctatttccag accatttccc ccctgcaccg cgcgccgctg 180

cccctgtctc ccaatccgga agactggccc acggtcgaca tcttcatccc gacctatgac 240

gaaagcctgg gcatcgtgcg tctgacggtg ctgggcgcgc ttggtatcga ctggccaccg 300

gacaaggtga acgtctatat ccttgatgac ggcaagcgtg aggaattcgc ccgctttgcc 360

gaagaatgcg gtgcccgcta cattgcccgt cccgataacg cgcatgccaa ggccggtaac 420

ctgaactacg ccattcagca tacaagtggc gaatacatcc tgattctgga ctgcgatcac 480

atcccgaccc gtgcgttcct gcagatctcg atgggatgga tggtcgagga caagaagatc 540

gccctgatgc agacgccgca tcacttctat tcccccgatc ctttccagcg taacctggcc 600

gtcggttacc gcacgccgcc tgaaggcaac ctgttctatg gtgtcattca ggatggcaac 660

gacttctggg atgcgacctt cttctgtggt tcctgtgcca tcctgcgccg caaggccatc 720

gaagagatca atggtttcgc aaccgagacc gtgacggaag atgcccatac cgccctgcgc 780

atgcagcgca gggggtggtc gaccgcctat ctgcgcattc cgctggccag cgggctggcg 840

acggagcgcc tggtcacgca tatcgggcag cgtatgcgct gggcccgtgg catgatccag 900

atcttccgcg tggataatcc catgctgggg ccgggcctga agctggggca gcggctttgc 960

tatctttcgg ccatgacgtc gttcttcttc gccattccgc gtgtcatctt ccttgcctcc 1020

ccgctggcct tccttttctt cagccagaat atcatcgcgg cctcccccct ggcggtgctg 1080

gcctacgcca ttccccacat gttccattcc gttgccacgg cggcaaaggt gaacaaggga 1140

tggcgctatt cattctggag tgaagtgtac gaaaccgtca tggcgctgtt cctggtgcgg 1200

gtgaccatcg tcacgatgat gttcccctcg aagggcaagt tcaacgtgac ggaaaaaggt 1260

ggcgttctgg agaacgagga attcgacctt ggtgccacat atccgaacat catctttgcg 1320

gtcatcatgg cgattggcct gatgcgcggg ctgtttgccc tggccttcca gcatctggac 1380

ataatttcag agcgtgccta cgcactcaac tgtgtctggt ccgtgatcag tctcatcatc 1440

ctgcttgcgg ccattgccgg cggccgtgag accaagcaga tccgccacag ccatcgtgtc 1500

gatgcgcgaa ttccggtaac ggtttatgat tacgaaggga attccagcca tggcatcacg 1560

caggacgtgt ccatgggtgg tgtggccatt catatgccgt ggcgcaatgt gacaccggac 1620

cagccggtgc agaccgttgt ccacgccgtg ctggatggtg aggtggtcaa tctccccgct 1680

accatgatcc gctgtgcgaa tggcaaggcg gtctttacct ggaacatcac ctccctcccg 1740

attgaagcct ctgtcgtccg gttcgtgttc ggtcgcgccg atgcctggct gcagtggaat 1800

gattacgagc atgatcggcc gttgcgaagc ctgtggagcc tgatcctcag catcaaggcg 1860

ctgttccgca agaagggtcg gatgatgatc catagtcgcc cgcaaaataa acccattgca 1920

ctgcctgttg agcgcaggga gccaacaagc agtcagggtg gtcagaaaca ggaaggaaag 1980

atcagtcgtg cggcctcgtg a 2001

<210> 2

<211> 666

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1824 (BcsA I)

<400> 2

Met Leu Ser Ala Leu Val Ser Leu Arg Tyr Leu Thr Trp Arg Leu Thr

1 5 10 15

Glu Thr Leu Asp Phe Asp Thr Trp Leu Gln Gly Thr Leu Gly Val Thr

20 25 30

Leu Leu Leu Ala Glu Leu Tyr Ala Leu Tyr Met Leu Phe Leu Ser Tyr

35 40 45

Phe Gln Thr Ile Ser Pro Leu His Arg Ala Pro Leu Pro Leu Ser Pro

50 55 60

Asn Pro Glu Asp Trp Pro Thr Val Asp Ile Phe Ile Pro Thr Tyr Asp

65 70 75 80

Glu Ser Leu Gly Ile Val Arg Leu Thr Val Leu Gly Ala Leu Gly Ile

85 90 95

Asp Trp Pro Pro Asp Lys Val Asn Val Tyr Ile Leu Asp Asp Gly Lys

100 105 110

Arg Glu Glu Phe Ala Arg Phe Ala Glu Glu Cys Gly Ala Arg Tyr Ile

115 120 125

Ala Arg Pro Asp Asn Ala His Ala Lys Ala Gly Asn Leu Asn Tyr Ala

130 135 140

Ile Gln His Thr Ser Gly Glu Tyr Ile Leu Ile Leu Asp Cys Asp His

145 150 155 160

Ile Pro Thr Arg Ala Phe Leu Gln Ile Ser Met Gly Trp Met Val Glu

165 170 175

Asp Lys Lys Ile Ala Leu Met Gln Thr Pro His His Phe Tyr Ser Pro

180 185 190

Asp Pro Phe Gln Arg Asn Leu Ala Val Gly Tyr Arg Thr Pro Pro Glu

195 200 205

Gly Asn Leu Phe Tyr Gly Val Ile Gln Asp Gly Asn Asp Phe Trp Asp

210 215 220

Ala Thr Phe Phe Cys Gly Ser Cys Ala Ile Leu Arg Arg Lys Ala Ile

225 230 235 240

Glu Glu Ile Asn Gly Phe Ala Thr Glu Thr Val Thr Glu Asp Ala His

245 250 255

Thr Ala Leu Arg Met Gln Arg Arg Gly Trp Ser Thr Ala Tyr Leu Arg

260 265 270

Ile Pro Leu Ala Ser Gly Leu Ala Thr Glu Arg Leu Val Thr His Ile

275 280 285

Gly Gln Arg Met Arg Trp Ala Arg Gly Met Ile Gln Ile Phe Arg Val

290 295 300

Asp Asn Pro Met Leu Gly Pro Gly Leu Lys Leu Gly Gln Arg Leu Cys

305 310 315 320

Tyr Leu Ser Ala Met Thr Ser Phe Phe Phe Ala Ile Pro Arg Val Ile

325 330 335

Phe Leu Ala Ser Pro Leu Ala Phe Leu Phe Phe Ser Gln Asn Ile Ile

340 345 350

Ala Ala Ser Pro Leu Ala Val Leu Ala Tyr Ala Ile Pro His Met Phe

355 360 365

His Ser Val Ala Thr Ala Ala Lys Val Asn Lys Gly Trp Arg Tyr Ser

370 375 380

Phe Trp Ser Glu Val Tyr Glu Thr Val Met Ala Leu Phe Leu Val Arg

385 390 395 400

Val Thr Ile Val Thr Met Met Phe Pro Ser Lys Gly Lys Phe Asn Val

405 410 415

Thr Glu Lys Gly Gly Val Leu Glu Asn Glu Glu Phe Asp Leu Gly Ala

420 425 430

Thr Tyr Pro Asn Ile Ile Phe Ala Val Ile Met Ala Ile Gly Leu Met

435 440 445

Arg Gly Leu Phe Ala Leu Ala Phe Gln His Leu Asp Ile Ile Ser Glu

450 455 460

Arg Ala Tyr Ala Leu Asn Cys Val Trp Ser Val Ile Ser Leu Ile Ile

465 470 475 480

Leu Leu Ala Ala Ile Ala Gly Gly Arg Glu Thr Lys Gln Ile Arg His

485 490 495

Ser His Arg Val Asp Ala Arg Ile Pro Val Thr Val Tyr Asp Tyr Glu

500 505 510

Gly Asn Ser Ser His Gly Ile Thr Gln Asp Val Ser Met Gly Gly Val

515 520 525

Ala Ile His Met Pro Trp Arg Asn Val Thr Pro Asp Gln Pro Val Gln

530 535 540

Thr Val Val His Ala Val Leu Asp Gly Glu Val Val Asn Leu Pro Ala

545 550 555 560

Thr Met Ile Arg Cys Ala Asn Gly Lys Ala Val Phe Thr Trp Asn Ile

565 570 575

Thr Ser Leu Pro Ile Glu Ala Ser Val Val Arg Phe Val Phe Gly Arg

580 585 590

Ala Asp Ala Trp Leu Gln Trp Asn Asp Tyr Glu His Asp Arg Pro Leu

595 600 605

Arg Ser Leu Trp Ser Leu Ile Leu Ser Ile Lys Ala Leu Phe Arg Lys

610 615 620

Lys Gly Arg Met Met Ile His Ser Arg Pro Gln Asn Lys Pro Ile Ala

625 630 635 640

Leu Pro Val Glu Arg Arg Glu Pro Thr Ser Ser Gln Gly Gly Gln Lys

645 650 655

Gln Glu Gly Lys Ile Ser Arg Ala Ala Ser

660 665

<210> 3

<211> 1131

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1824 (BcsB I)

<400> 3

gtgcgcttcc gggcgcccga cgggccgatc gtggacctgg cgcgttcgca tctggacgtt 60

ggtatcaaca atacctacct gcagtcctat tccctgcatg aaaaggacag tgtggtcgac 120

cagctggtcc agcgttttgg cggccggggc cagaccagtg gcgtgcagca gcatacgctg 180

accattccgc cgtggatggt gttcggtcag gatcagctgc agttctattt tgatgcggcc 240

cccctgaccc agcccggctg ccgtcccggc cccagcctga tccacatgtc ggttgatccg 300

gattccacga tcgacctgtc caacgcctat cacatcacgc gcatgcccaa tctggcctac 360

atggccagcg cggggtatcc gttcaccacc tatgccgacc tgtcgcactc ggccgtggtg 420

ctgccggacc atcccaatgg tacggttgtc agcgcctatc ttgacctgat gggcttcatg 480

ggggcgacga cgtggtatcc cgtctcgggt ctggacatcg tttccgcgga tcatgtgaat 540

gatgtggcgg accggaacct gatcgtcctg tccacgctgg ccaatagcgg ggaggtttcc 600

tccctgctgt cgaactcgtc gtaccagatt gccgacgggc gcctgcacat ggggatgcgc 660

tccaccctga gtggggtgtg gaacatcttc caggacccga tggccgccat caacaatacc 720

catccgaccg aggtcgagac gaccctgagc ggtggcgtgg gcgcgatggt ggaagcggaa 780

tccccgctgg catccggacg cacggttctt gccctgctct cggctgacgg gcaggggctg 840

gacaatctgg tccagatcct cgggcagcgt aagaaccagg ccaagattca gggcgacctg 900

gtgcttgccc atggggatga cctgacatcg taccgcagtt cgccccttta taccgttggc 960

acgctgccga tgtggctcat gccggactgg tatatgcata accatcccgt tcgcgtgatc 1020

gtggtggggc tgttcggatg cctgctggtt gtggccgtgc tggttcgtgc cctgttgcgg 1080

catgcactgt tccgccggcg gcagctgcag gaagaaaggc agaaatcgtg a 1131

<210> 4

<211> 376

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1824 (BcsB I)

<400> 4

Met Arg Phe Arg Ala Pro Asp Gly Pro Ile Val Asp Leu Ala Arg Ser

1 5 10 15

His Leu Asp Val Gly Ile Asn Asn Thr Tyr Leu Gln Ser Tyr Ser Leu

20 25 30

His Glu Lys Asp Ser Val Val Asp Gln Leu Val Gln Arg Phe Gly Gly

35 40 45

Arg Gly Gln Thr Ser Gly Val Gln Gln His Thr Leu Thr Ile Pro Pro

50 55 60

Trp Met Val Phe Gly Gln Asp Gln Leu Gln Phe Tyr Phe Asp Ala Ala

65 70 75 80

Pro Leu Thr Gln Pro Gly Cys Arg Pro Gly Pro Ser Leu Ile His Met

85 90 95

Ser Val Asp Pro Asp Ser Thr Ile Asp Leu Ser Asn Ala Tyr His Ile

100 105 110

Thr Arg Met Pro Asn Leu Ala Tyr Met Ala Ser Ala Gly Tyr Pro Phe

115 120 125

Thr Thr Tyr Ala Asp Leu Ser His Ser Ala Val Val Leu Pro Asp His

130 135 140

Pro Asn Gly Thr Val Val Ser Ala Tyr Leu Asp Leu Met Gly Phe Met

145 150 155 160

Gly Ala Thr Thr Trp Tyr Pro Val Ser Gly Leu Asp Ile Val Ser Ala

165 170 175

Asp His Val Asn Asp Val Ala Asp Arg Asn Leu Ile Val Leu Ser Thr

180 185 190

Leu Ala Asn Ser Gly Glu Val Ser Ser Leu Leu Ser Asn Ser Ser Tyr

195 200 205

Gln Ile Ala Asp Gly Arg Leu His Met Gly Met Arg Ser Thr Leu Ser

210 215 220

Gly Val Trp Asn Ile Phe Gln Asp Pro Met Ala Ala Ile Asn Asn Thr

225 230 235 240

His Pro Thr Glu Val Glu Thr Thr Leu Ser Gly Gly Val Gly Ala Met

245 250 255

Val Glu Ala Glu Ser Pro Leu Ala Ser Gly Arg Thr Val Leu Ala Leu

260 265 270

Leu Ser Ala Asp Gly Gln Gly Leu Asp Asn Leu Val Gln Ile Leu Gly

275 280 285

Gln Arg Lys Asn Gln Ala Lys Ile Gln Gly Asp Leu Val Leu Ala His

290 295 300

Gly Asp Asp Leu Thr Ser Tyr Arg Ser Ser Pro Leu Tyr Thr Val Gly

305 310 315 320

Thr Leu Pro Met Trp Leu Met Pro Asp Trp Tyr Met His Asn His Pro

325 330 335

Val Arg Val Ile Val Val Gly Leu Phe Gly Cys Leu Leu Val Val Ala

340 345 350

Val Leu Val Arg Ala Leu Leu Arg His Ala Leu Phe Arg Arg Arg Gln

355 360 365

Leu Gln Glu Glu Arg Gln Lys Ser

370 375

<210> 5

<211> 1272

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1825 (BcsB I)

<400> 5

gtgtccctga tcgcgctgct ggtctttgca acgggagcgc aggctgcgcc ggttgcatcc 60

aaagcgccag ccccgcagcc tgcgggcgat aacctgccgc ccctgcccgc cgcggcaccg 120

gccgccgcgg cagccccggc cgggcagcag cctgctggcg ccgccagtgc ggcacctgcc 180

gtcgatccgg ccgctgccag cgccgccgat gccatggtgg acaatgcgga gaatgcgacc 240

ggcgtcggtt cggatgtggc gaccgtgcat acctattccc tgcgcgaact tggcgcggag 300

aacgcgctga ccatgcgtgg cgcggccccc ctgcaggggc tgcagttcgg tattccgggc 360

gaccagctcg tcacctcggc gcggcttgtc gtgtcgggtg cgatgtcacc caatctccag 420

cccgataaca gcgcggtcac gattacgctg aacgagcagt atatcggcac gctccggcct 480

gacccgtcac atccggcctt tggtccgctt tcctttgaca tcaaccccat cttctttgtc 540

agcggcaacc ggctgaactt caatttctcg gcagggtcga aaggatgcac cgacccgagc 600

aacggattgc agtgggccag cgtgtccgag cattcggaac tgcagatcac caccataccg 660

cttcctcccc gtcgtcagct gtcgcggctg ccgcagccgt tctttgacaa gaacgtaagg 720

cagaagacgg tcattccgtt cgtccttgca cagacatttg atgctgaagt gctcaaggct 780

tccggcatcc tggcgtcctg gttcggccag cagaccgatt tccgcggcgt gaacttcccc 840

gtattttcca ccattccgca gacaggcaat gccgttgtgg tgggtgttgc cgatgaactg 900

ccttccgcgc tggggcgtcc ggccatcagc gggccgaccc tgatggaagt ggccaacccg 960

tccgatccca atggcacgat cctgctggta acgggccggg accgcgatga agtcattacc 1020

gcaagcaagg gcataggctt cagctccagc acgctgccgg ttgccgcgcg catggatgtg 1080

gcgccgattg acgtggcccc cgcgccccca acgacgcgcc gtccttcatc ccgaccagcc 1140

ggcctgtccg gctgggtgaa ctggtgccgg tcagtgccct gcagggcgaa ggctataccc 1200

ccggcgtgct ttccgtggcg ttccgcacgg cgcctgacct gtatacctgg cgcgaccggc 1260

cgtacaagct ga 1272

<210> 6

<211> 423

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1825 (BcsB I)

<400> 6

Met Ser Leu Ile Ala Leu Leu Val Phe Ala Thr Gly Ala Gln Ala Ala

1 5 10 15

Pro Val Ala Ser Lys Ala Pro Ala Pro Gln Pro Ala Gly Asp Asn Leu

20 25 30

Pro Pro Leu Pro Ala Ala Ala Pro Ala Ala Ala Ala Ala Pro Ala Gly

35 40 45

Gln Gln Pro Ala Gly Ala Ala Ser Ala Ala Pro Ala Val Asp Pro Ala

50 55 60

Ala Ala Ser Ala Ala Asp Ala Met Val Asp Asn Ala Glu Asn Ala Thr

65 70 75 80

Gly Val Gly Ser Asp Val Ala Thr Val His Thr Tyr Ser Leu Arg Glu

85 90 95

Leu Gly Ala Glu Asn Ala Leu Thr Met Arg Gly Ala Ala Pro Leu Gln

100 105 110

Gly Leu Gln Phe Gly Ile Pro Gly Asp Gln Leu Val Thr Ser Ala Arg

115 120 125

Leu Val Val Ser Gly Ala Met Ser Pro Asn Leu Gln Pro Asp Asn Ser

130 135 140

Ala Val Thr Ile Thr Leu Asn Glu Gln Tyr Ile Gly Thr Leu Arg Pro

145 150 155 160

Asp Pro Ser His Pro Ala Phe Gly Pro Leu Ser Phe Asp Ile Asn Pro

165 170 175

Ile Phe Phe Val Ser Gly Asn Arg Leu Asn Phe Asn Phe Ser Ala Gly

180 185 190

Ser Lys Gly Cys Thr Asp Pro Ser Asn Gly Leu Gln Trp Ala Ser Val

195 200 205

Ser Glu His Ser Glu Leu Gln Ile Thr Thr Ile Pro Leu Pro Pro Arg

210 215 220

Arg Gln Leu Ser Arg Leu Pro Gln Pro Phe Phe Asp Lys Asn Val Arg

225 230 235 240

Gln Lys Thr Val Ile Pro Phe Val Leu Ala Gln Thr Phe Asp Ala Glu

245 250 255

Val Leu Lys Ala Ser Gly Ile Leu Ala Ser Trp Phe Gly Gln Gln Thr

260 265 270

Asp Phe Arg Gly Val Asn Phe Pro Val Phe Ser Thr Ile Pro Gln Thr

275 280 285

Gly Asn Ala Val Val Val Gly Val Ala Asp Glu Leu Pro Ser Ala Leu

290 295 300

Gly Arg Pro Ala Ile Ser Gly Pro Thr Leu Met Glu Val Ala Asn Pro

305 310 315 320

Ser Asp Pro Asn Gly Thr Ile Leu Leu Val Thr Gly Arg Asp Arg Asp

325 330 335

Glu Val Ile Thr Ala Ser Lys Gly Ile Gly Phe Ser Ser Ser Thr Leu

340 345 350

Pro Val Ala Ala Arg Met Asp Val Ala Pro Ile Asp Val Ala Pro Ala

355 360 365

Pro Pro Thr Thr Arg Arg Pro Ser Ser Arg Pro Ala Gly Leu Ser Gly

370 375 380

Trp Val Asn Trp Cys Arg Ser Val Pro Cys Arg Ala Lys Ala Ile Pro

385 390 395 400

Pro Ala Cys Phe Pro Trp Arg Ser Ala Arg Arg Leu Thr Cys Ile Pro

405 410 415

Gly Ala Thr Gly Arg Thr Ser

420

<210> 7

<211> 3981

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1823 (BcsC I)

<400> 7

gtgagcatga acagacgcta cgtcttttcc ctttctgccg gcctgcttgc cagcagttgc 60

atgaccgtgc tggtggcggt gccactggcg cgcgcgcagc aggcctccac ggccatgacc 120

ggtacccagg cttcgggcgg gtcggcggcg ccacggcaga tcctgctgca gcaggcccgg 180

ttctggcttc agcagcagca gtatgacaat gcccgtcagg ccctgcagaa tgcccagcgc 240

gtggcgccgg atgcgccgga tgtcctggag gtacagggcg aataccagac ggcgatcggc 300

aaccgggaag cggcagccga tacgctgcgc cacctccagc aggttgcgcc cggcagtacg 360

gccgccaaca gcctgagtga cctgttgcac gagcgttcca tctcgacatc cgacctgtcg 420

caggtgcgtt cccttgccgc atccgggcat aacgcgcagg cggtgcaggg gtaccagaag 480

ctgttcaatg gcggtaagcc gccgcattcg cttgcggtgg aatattacca gaccatggca 540

ggcgttccgg ccgaatggga tcaggcccgg gccgggcttg ccggtatcgt ggcatccaat 600

ccacaggatt atcatgccca gctcgcattt gcgcaggcgc tgacctataa tacggcgacc 660

cgtatggaag gtctggcgcg gctcaaggac ctgcagggtt tccgcagcca ggctccggtc 720

gaggctgcgg ccgccagcca gtcctaccgg cagacgctga gctggctgcc ggtaacgccc 780

accacgcagc cgctcatgca gcagtggctg gatagccatc ccaatgatac cgaactgcgt 840

gagcatatgg tccacccgcc cggcggcccg ccggacaagg cgggtcttgc gcgtcaggcg 900

ggttatcagc agctgaatgc cggccgtatt gccgcagccg agcagtcctt ccagtccgcg 960

ttacagatca attcccatga tgccgattca cttggcggca tgggactggt cagcatgcgg 1020

cagggtgacg cagccgaagc ccgccgctat ttccaggaag cgatggcggc cgatcccaag 1080

acggcggatc gctggcgccc ggccctggcc ggcatggaaa tcagcggtga ctatgccgcg 1140

gtccgccagc ttattgccgc ccaccagtat gatgcggcca agcagcgcct gtccgcgctg 1200

gcacgccagt ccggccagtt taccggcgcc acgctcatgc tggccgacct gcagcgcacg 1260

accggccaga tgggtgcggc ggagcaggaa taccagtccg ttctggcacg cgacccgaac 1320

agccagcttg ccctgatggg actggcgcgg gtggagatgg cgcagggcaa gacggcggaa 1380

gcccgccagc tgctgtcgcg tgtcggatcg cagtatgcga cccaggtcgg ggaaatcgag 1440

gtgacgggcc ttatggccgc cgcctcgcag acatcggatt ccgcgcgcaa ggtctcgatc 1500

ctgcgcgaag ccatggccca ggcaccgcgt gacccatggg tgcggatcaa tctggccaat 1560

gccctgcagc agcaggggga catggcggaa gccaatcggg tcatgcagcc catcctgtcc 1620

aatcccgtga cggcgcagga ccgacaggcc ggtatcctgt ttacctatgg cagtggcaat 1680

gatgcgatga cacgccgcct gctggctggc ctgtcgcccg aggactattc ccccgccatc 1740

catgccattg cgacggaaat ggagatcaag caggatctgg ccagccgcct gtccatggtg 1800

gcgaacccgg ttccgctgat ccgtgaagcc ctttcgccgc ccgacccgac gggcgcgcgt 1860

ggcgtggccg tggctgatct gttccgtcag cgtggcgaca tgattcatgc ccgcatggcc 1920

ctgcgcattg cctcgacccg cacgctcgat ctttcggcgg accagcgtct ggcctacgcc 1980

accgaataca tgaagatcag caacccggtt gcggccgccc gcctgctggc cccgctgggt 2040

gacggcagtg gcacgggggc gggcaatgcc ctgcttcccg agcaggtaca gacgctgcag 2100

cagctgcgca tggggattgc cgtggcccag tccgacctgc tgaaccagcg cggcgatcag 2160

gcgcaggcat acgatcacct tgctccggcc ctgcaggccg atccggaagc gacatcgccc 2220

aaactggcgc tggcgcggct ttacaatggt cagggcaagt ccggcaaggc gctggaaatc 2280

gatctggccg tgctgcggca caacccgcag gatctggatg cgcgccaggc agcggtgcag 2340

gctgccgtca atagcggccg caagagcctg gccacccgcc ttgccatgga tggtgtgcag 2400

gaaagcccga tggatgcgcg tgcctggctg gccatggccg tggccgatca ggccgatggc 2460

catggccacc ggaccatcag tgacctgcgc cgcgcctatg acctgcgtct gcagcaggtg 2520

gaaggcacgc gggcggtggc aagcgggacg ggtgagcagg aatcgcttga acccccgtcc 2580

agcaacccgt ttcgccacca tggctatgga cgccagacgg aactgggcgc accggttacg 2640

ggtggctcct acagcatgga ggcaacgtct cccgaagcat cggaccagat gctgtcctcc 2700

atcgccgggc agatcaccac gctgcgggaa aacctggccc cctccatcga tggcggtctg 2760

gggttccggt cgcgttcggg tgagcacggc atgggccgcc tgaccgaagc gaacattccc 2820

atcgtggggc gcctgccgct gcaggcgggt gagtccagcc tgaccttctc gatcacgcca 2880

accatgatct ggtcgggaca gctcaatacc ggttcggtct atgatgtgcc gcgctttggc 2940

accgacatgg caacacaggc gtataaccag tacgtcagtt acataagcca gaacaattcc 3000

agcagcaccc tgcatagcga acttgtcaag ggtggcgagg ccgaggccgg ttttgcgcca 3060

gacgtgcagt tcggcaacag ctgggtgcgg gcggacctgg gggcatcgcc catcggcttc 3120

cccatcacca acgtactggg cggtgtggaa ttctcgccgc gtgtcggacc ggttaccttc 3180

cgcgtcagcg cggaacgccg ctccatcacc aacagcgtgc tgtcctatgg tggcatgcgc 3240

gaccccaact acaacacgac actgggccgc tatgcccgcc agctttatgg caaggagctg 3300

agttcccagt ggagtgagga atggggcggg gtcgtgacca accacttcca tggtcaggtt 3360

gaggcaacgc tgggcaacac catcgtatat ggtggcggtg gctatgccat ccagaccggc 3420

aagcatgtgc agcgcaatga cgagcgcgag gcgggcatcg gtgtcaacac gctggtctgg 3480

cacaatgcca acatgctggt ccgcatcggt gtcagcctga cctatttcgg ctatgccaac 3540

aaccaggact tctataccta cgggcagggt ggctacttct cgccgcaatc ctattacgcg 3600

gcgaccgtac ccatccggta tgcggggcag cacaagcggc tggactggga cgtgacgggc 3660

agcgttggct accaggtgtt ccatgaacac tcgtccccat tcttcccgac gtcttccctg 3720

ctgcagtctg gcgcgcagta cattgctgac tcgtatgtgc agaacgcaac cagttccgac 3780

tatctctcac aggagacggt caacagcgcc tattatcccg gagatagtat tgctagtctt 3840

acgggtggct tcaatgctag ggtagggtat cgatttacac acaatcttcg tcttgatctg 3900

tcggggcgct ggcagaaggc cggtaactgg actgaaagcg gcgccatgat ttccgcacac 3960

tatcttatta tggaccagta a 3981

<210> 8

<211> 1326

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1823 (BcsC I)

<400> 8

Met Ser Met Asn Arg Arg Tyr Val Phe Ser Leu Ser Ala Gly Leu Leu

1 5 10 15

Ala Ser Ser Cys Met Thr Val Leu Val Ala Val Pro Leu Ala Arg Ala

20 25 30

Gln Gln Ala Ser Thr Ala Met Thr Gly Thr Gln Ala Ser Gly Gly Ser

35 40 45

Ala Ala Pro Arg Gln Ile Leu Leu Gln Gln Ala Arg Phe Trp Leu Gln

50 55 60

Gln Gln Gln Tyr Asp Asn Ala Arg Gln Ala Leu Gln Asn Ala Gln Arg

65 70 75 80

Val Ala Pro Asp Ala Pro Asp Val Leu Glu Val Gln Gly Glu Tyr Gln

85 90 95

Thr Ala Ile Gly Asn Arg Glu Ala Ala Ala Asp Thr Leu Arg His Leu

100 105 110

Gln Gln Val Ala Pro Gly Ser Thr Ala Ala Asn Ser Leu Ser Asp Leu

115 120 125

Leu His Glu Arg Ser Ile Ser Thr Ser Asp Leu Ser Gln Val Arg Ser

130 135 140

Leu Ala Ala Ser Gly His Asn Ala Gln Ala Val Gln Gly Tyr Gln Lys

145 150 155 160

Leu Phe Asn Gly Gly Lys Pro Pro His Ser Leu Ala Val Glu Tyr Tyr

165 170 175

Gln Thr Met Ala Gly Val Pro Ala Glu Trp Asp Gln Ala Arg Ala Gly

180 185 190

Leu Ala Gly Ile Val Ala Ser Asn Pro Gln Asp Tyr His Ala Gln Leu

195 200 205

Ala Phe Ala Gln Ala Leu Thr Tyr Asn Thr Ala Thr Arg Met Glu Gly

210 215 220

Leu Ala Arg Leu Lys Asp Leu Gln Gly Phe Arg Ser Gln Ala Pro Val

225 230 235 240

Glu Ala Ala Ala Ala Ser Gln Ser Tyr Arg Gln Thr Leu Ser Trp Leu

245 250 255

Pro Val Thr Pro Thr Thr Gln Pro Leu Met Gln Gln Trp Leu Asp Ser

260 265 270

His Pro Asn Asp Thr Glu Leu Arg Glu His Met Val His Pro Pro Gly

275 280 285

Gly Pro Pro Asp Lys Ala Gly Leu Ala Arg Gln Ala Gly Tyr Gln Gln

290 295 300

Leu Asn Ala Gly Arg Ile Ala Ala Ala Glu Gln Ser Phe Gln Ser Ala

305 310 315 320

Leu Gln Ile Asn Ser His Asp Ala Asp Ser Leu Gly Gly Met Gly Leu

325 330 335

Val Ser Met Arg Gln Gly Asp Ala Ala Glu Ala Arg Arg Tyr Phe Gln

340 345 350

Glu Ala Met Ala Ala Asp Pro Lys Thr Ala Asp Arg Trp Arg Pro Ala

355 360 365

Leu Ala Gly Met Glu Ile Ser Gly Asp Tyr Ala Ala Val Arg Gln Leu

370 375 380

Ile Ala Ala His Gln Tyr Asp Ala Ala Lys Gln Arg Leu Ser Ala Leu

385 390 395 400

Ala Arg Gln Ser Gly Gln Phe Thr Gly Ala Thr Leu Met Leu Ala Asp

405 410 415

Leu Gln Arg Thr Thr Gly Gln Met Gly Ala Ala Glu Gln Glu Tyr Gln

420 425 430

Ser Val Leu Ala Arg Asp Pro Asn Ser Gln Leu Ala Leu Met Gly Leu

435 440 445

Ala Arg Val Glu Met Ala Gln Gly Lys Thr Ala Glu Ala Arg Gln Leu

450 455 460

Leu Ser Arg Val Gly Ser Gln Tyr Ala Thr Gln Val Gly Glu Ile Glu

465 470 475 480

Val Thr Gly Leu Met Ala Ala Ala Ser Gln Thr Ser Asp Ser Ala Arg

485 490 495

Lys Val Ser Ile Leu Arg Glu Ala Met Ala Gln Ala Pro Arg Asp Pro

500 505 510

Trp Val Arg Ile Asn Leu Ala Asn Ala Leu Gln Gln Gln Gly Asp Met

515 520 525

Ala Glu Ala Asn Arg Val Met Gln Pro Ile Leu Ser Asn Pro Val Thr

530 535 540

Ala Gln Asp Arg Gln Ala Gly Ile Leu Phe Thr Tyr Gly Ser Gly Asn

545 550 555 560

Asp Ala Met Thr Arg Arg Leu Leu Ala Gly Leu Ser Pro Glu Asp Tyr

565 570 575

Ser Pro Ala Ile His Ala Ile Ala Thr Glu Met Glu Ile Lys Gln Asp

580 585 590

Leu Ala Ser Arg Leu Ser Met Val Ala Asn Pro Val Pro Leu Ile Arg

595 600 605

Glu Ala Leu Ser Pro Pro Asp Pro Thr Gly Ala Arg Gly Val Ala Val

610 615 620

Ala Asp Leu Phe Arg Gln Arg Gly Asp Met Ile His Ala Arg Met Ala

625 630 635 640

Leu Arg Ile Ala Ser Thr Arg Thr Leu Asp Leu Ser Ala Asp Gln Arg

645 650 655

Leu Ala Tyr Ala Thr Glu Tyr Met Lys Ile Ser Asn Pro Val Ala Ala

660 665 670

Ala Arg Leu Leu Ala Pro Leu Gly Asp Gly Ser Gly Thr Gly Ala Gly

675 680 685

Asn Ala Leu Leu Pro Glu Gln Val Gln Thr Leu Gln Gln Leu Arg Met

690 695 700

Gly Ile Ala Val Ala Gln Ser Asp Leu Leu Asn Gln Arg Gly Asp Gln

705 710 715 720

Ala Gln Ala Tyr Asp His Leu Ala Pro Ala Leu Gln Ala Asp Pro Glu

725 730 735

Ala Thr Ser Pro Lys Leu Ala Leu Ala Arg Leu Tyr Asn Gly Gln Gly

740 745 750

Lys Ser Gly Lys Ala Leu Glu Ile Asp Leu Ala Val Leu Arg His Asn

755 760 765

Pro Gln Asp Leu Asp Ala Arg Gln Ala Ala Val Gln Ala Ala Val Asn

770 775 780

Ser Gly Arg Lys Ser Leu Ala Thr Arg Leu Ala Met Asp Gly Val Gln

785 790 795 800

Glu Ser Pro Met Asp Ala Arg Ala Trp Leu Ala Met Ala Val Ala Asp

805 810 815

Gln Ala Asp Gly His Gly His Arg Thr Ile Ser Asp Leu Arg Arg Ala

820 825 830

Tyr Asp Leu Arg Leu Gln Gln Val Glu Gly Thr Arg Ala Val Ala Ser

835 840 845

Gly Thr Gly Glu Gln Glu Ser Leu Glu Pro Pro Ser Ser Asn Pro Phe

850 855 860

Arg His His Gly Tyr Gly Arg Gln Thr Glu Leu Gly Ala Pro Val Thr

865 870 875 880

Gly Gly Ser Tyr Ser Met Glu Ala Thr Ser Pro Glu Ala Ser Asp Gln

885 890 895

Met Leu Ser Ser Ile Ala Gly Gln Ile Thr Thr Leu Arg Glu Asn Leu

900 905 910

Ala Pro Ser Ile Asp Gly Gly Leu Gly Phe Arg Ser Arg Ser Gly Glu

915 920 925

His Gly Met Gly Arg Leu Thr Glu Ala Asn Ile Pro Ile Val Gly Arg

930 935 940

Leu Pro Leu Gln Ala Gly Glu Ser Ser Leu Thr Phe Ser Ile Thr Pro

945 950 955 960

Thr Met Ile Trp Ser Gly Gln Leu Asn Thr Gly Ser Val Tyr Asp Val

965 970 975

Pro Arg Phe Gly Thr Asp Met Ala Thr Gln Ala Tyr Asn Gln Tyr Val

980 985 990

Ser Tyr Ile Ser Gln Asn Asn Ser Ser Ser Thr Leu His Ser Glu Leu

995 1000 1005

Val Lys Gly Gly Glu Ala Glu Ala Gly Phe Ala Pro Asp Val Gln Phe

1010 1015 1020

Gly Asn Ser Trp Val Arg Ala Asp Leu Gly Ala Ser Pro Ile Gly Phe

1025 1030 1035 1040

Pro Ile Thr Asn Val Leu Gly Gly Val Glu Phe Ser Pro Arg Val Gly

1045 1050 1055

Pro Val Thr Phe Arg Val Ser Ala Glu Arg Arg Ser Ile Thr Asn Ser

1060 1065 1070

Val Leu Ser Tyr Gly Gly Met Arg Asp Pro Asn Tyr Asn Thr Thr Leu

1075 1080 1085

Gly Arg Tyr Ala Arg Gln Leu Tyr Gly Lys Glu Leu Ser Ser Gln Trp

1090 1095 1100

Ser Glu Glu Trp Gly Gly Val Val Thr Asn His Phe His Gly Gln Val

1105 1110 1115 1120

Glu Ala Thr Leu Gly Asn Thr Ile Val Tyr Gly Gly Gly Gly Tyr Ala

1125 1130 1135

Ile Gln Thr Gly Lys His Val Gln Arg Asn Asp Glu Arg Glu Ala Gly

1140 1145 1150

Ile Gly Val Asn Thr Leu Val Trp His Asn Ala Asn Met Leu Val Arg

1155 1160 1165

Ile Gly Val Ser Leu Thr Tyr Phe Gly Tyr Ala Asn Asn Gln Asp Phe

1170 1175 1180

Tyr Thr Tyr Gly Gln Gly Gly Tyr Phe Ser Pro Gln Ser Tyr Tyr Ala

1185 1190 1195 1200

Ala Thr Val Pro Ile Arg Tyr Ala Gly Gln His Lys Arg Leu Asp Trp

1205 1210 1215

Asp Val Thr Gly Ser Val Gly Tyr Gln Val Phe His Glu His Ser Ser

1220 1225 1230

Pro Phe Phe Pro Thr Ser Ser Leu Leu Gln Ser Gly Ala Gln Tyr Ile

1235 1240 1245

Ala Asp Ser Tyr Val Gln Asn Ala Thr Ser Ser Asp Tyr Leu Ser Gln

1250 1255 1260

Glu Thr Val Asn Ser Ala Tyr Tyr Pro Gly Asp Ser Ile Ala Ser Leu

1265 1270 1275 1280

Thr Gly Gly Phe Asn Ala Arg Val Gly Tyr Arg Phe Thr His Asn Leu

1285 1290 1295

Arg Leu Asp Leu Ser Gly Arg Trp Gln Lys Ala Gly Asn Trp Thr Glu

1300 1305 1310

Ser Gly Ala Met Ile Ser Ala His Tyr Leu Ile Met Asp Gln

1315 1320 1325

<210> 9

<211> 471

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1822 (BcsD I)

<400> 9

atgacaactt tcaacgcaaa accggacttt tccctgttcc tgcaggccct ctcctgggag 60

attgatgatc aggccgggat cgaggtgagg aatgacctgt tgcgcgaggt cggtcacggc 120

atggccggtc ggctgcagcc tccgctgtgc aacaccattc atcagctgca gatcgagctg 180

aactcgctgc tggccatgat caactggggc tatgtgcagc ttgaactgct gcccgaggac 240

catgccatgc gcatcgtcca tgaggacctg ccccaggtgg gcagcgcggg cgagccggcc 300

ggcacatggc tggcccccgt gctcgaaggg ctgtatggcc gctggatcac gtcgcagccg 360

ggtgcctttg gcgattatgt cgtcacccgc gatgtggatg cggaggatct caactccgtt 420

cccagccaga cgatcatcct gtacatgcgc acccgcagca gcagcaactg a 471

<210> 10

<211> 156

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1822 (BcsD I)

<400> 10

Met Thr Thr Phe Asn Ala Lys Pro Asp Phe Ser Leu Phe Leu Gln Ala

1 5 10 15

Leu Ser Trp Glu Ile Asp Asp Gln Ala Gly Ile Glu Val Arg Asn Asp

20 25 30

Leu Leu Arg Glu Val Gly His Gly Met Ala Gly Arg Leu Gln Pro Pro

35 40 45

Leu Cys Asn Thr Ile His Gln Leu Gln Ile Glu Leu Asn Ser Leu Leu

50 55 60

Ala Met Ile Asn Trp Gly Tyr Val Gln Leu Glu Leu Leu Pro Glu Asp

65 70 75 80

His Ala Met Arg Ile Val His Glu Asp Leu Pro Gln Val Gly Ser Ala

85 90 95

Gly Glu Pro Ala Gly Thr Trp Leu Ala Pro Val Leu Glu Gly Leu Tyr

100 105 110

Gly Arg Trp Ile Thr Ser Gln Pro Gly Ala Phe Gly Asp Tyr Val Val

115 120 125

Thr Arg Asp Val Asp Ala Glu Asp Leu Asn Ser Val Pro Ser Gln Thr

130 135 140

Ile Ile Leu Tyr Met Arg Thr Arg Ser Ser Ser Asn

145 150 155

<210> 11

<211> 2793

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3004 (BcsAB II)

<400> 11

atggcgcagt ttgtggataa aattaccaga ttcatggagc aggcggctac cggcagggtg 60

ccttcatggg tgccgatcgt gatcggtgtc gtcatgatga gctttgtggg ttcggttgca 120

ttgctgccgg acatgcaggg gctgatttca atcgggacgg tcctgctgct gcttgtgctg 180

aaccggttca aggggcgcgg catcaccata ttcctcatga tgctctcgct gctggtgtcc 240

atgcgttacg tggtctggcg gctgacatcg acgatcgagt tccatgggtg gatacagtcc 300

gccctgtcca tcctgctgct cctggccgag gtttatgccc tgtccaccct gtgcctgagc 360

tatttccaga tggcctggcc gctggggcgc aaggagcatc ccctgcccga ggatacgtcc 420

tcgtggccac atgtggacat atatgttcca tcatataacg aggaactgtc cctggtccgc 480

tcgaccgtgc tgggcgcgct gaagcttgac tggccagagg acaagcttca cgtctacatc 540

cttgacgacg ggcgccgggt cgcctttcgt gactttgcgc tggaagcggg ggcgggctac 600

atcatccgct cgcaaaacaa ccatgcgaaa gcaggcaacc tcaatcatgc gctgaagatt 660

accgatggcc agttcgccgt gatcttcgac tgcgatcatg tgcccacgcg cggcttcctg 720

aaaagaacca tcggctggat gatcgccgat cccaagcttg ccctgctgca gaccccgcac 780

catttctatg cgcccgaccc gttccagcgc aatcttgtgg cgggtgccca tgtccccccc 840

gaagggaaca tgttctatgg tctggtgcag gatggcaatg atttctggga cgcaacattc 900

ttctgcggtt cctgcgcggt catccgccgc tccgctgttc tgggcattgg cggctttgca 960

accgagacgg tgacggaaga cgcgcatacc gcgctcaaga tgcagcgcag gggctggcgg 1020

accgcctacc tgcgtgaacc gctggccggc gggcttgcga ccgaacggct gatcctgcat 1080

atcggccagc gcgtgcgctg ggcgcgtggc atgctgcaga tcatgcggcg ggacaatccc 1140

ctgctggggc gcgggctgcg gtgggagcag cggctgtgtt acctgtccgc catgtcgcac 1200

ttcctgttcg ccattccgcg cgtgaccttc cttgtctcgc cgctggcgta cctgtttctg 1260

gggcagaata tcatcgcggc ctcgccactg gccatcagtg tctatgcgct gccgcacatc 1320

ttccattcca tcctgaccct gtcgcgtatt gaaagccgct ggcggtattc attctggagc 1380

gagatctacg aaacctcgct cgcccttttc ctggtcagga tcaccatcgt cacgctgctg 1440

cagccccaca aggggacctt taacgtgacg gacaagggcg gcctgctgga aaaaagctat 1500

tttgacgtgg gggccgtcta tcccaacgtc atccttgcgg tcatcctgtt cgccgcattc 1560

ctgcggggca tattcggcat tgtatggcag ttccatgacc gcctggccct gcagtccttc 1620

gcgctcaata cgctgtgggt ggtcataagc ctgatcatcg tgctggcttc catcgcggtc 1680

gggcgtgaaa cacgccagac ccgtgccgcc ccgcgcattg ccgtggccct gccggtcagg 1740

ataaccgaca tggaaggccg gagctttgcc ggacataccc gcgatatctc gctgggtgga 1800

ctgggggttg acctgcactg gcccgcagat gtcgcacggc ccgacagggt catgatggaa 1860

tatgtaaacg agcgggacgg gatccatgcc accgtgcccg caacggtcct ggccctggat 1920

gaacgttcca tgcggctgca atgggaacgc cgcgatctgg aagatgaaag ccagatcgtg 1980

gacatggtct ttggccggaa tgatgcctgg gcgaactggg ccgatttcga gcccgatcgc 2040

cccctgcgca gcattgccat ggtgctcaga agtattggtg gcctttttaa atggcaggcg 2100

cgtgagatcc cacgccatgt ggcggatgac gaagaggcca aggcgcctgt cgtggaagca 2160

aaactggaga aacaaagcct cgtgctgaaa cctgttcgac gcagtgcccg gaatggtgct 2220

gccgcatccg ctgtccttct cgtggctctt gccgccctca gcccggtcgc gatggcgcag 2280

gtgcagcccg ccgcgtccgc ttccgtgccg gaccagacgg gcgtcagcgc cgaaaccccg 2340

ttcggggaca gcaataccgg gaccgtgccg gacatgattc cggttatcga tcaggcggcg 2400

gcggaccgga tcagtgattc ggaagtgacc cgcaccctgt ccttccgcaa tctgggggcc 2460

acgtcaggcc cgctgacgct gcgtggctat tcccccctgc aggggctgga tgtgatcgtg 2520

cccgccaaca gggtggtcac gcatgcgcgg ctgtccattt ccggcgccct ttcgccctcc 2580

ctgctgccgg aagccagcgc ggttacggtc acgctgaacg agcagtatat cggcacgatc 2640

aaggtcgatc ccgaacaccc gcagttcggg ccgctgacat tcgatatcga cccgctgtat 2700

ttcacgggcg acaacaagct caatttccgt tttgcgggcg aataccggcg tgactgtaaa 2760

gaggtggtcc ccatttttcg gacagggcga taa 2793

<210> 12

<211> 930

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3004 (BcsAB II)

<400> 12

Met Ala Gln Phe Val Asp Lys Ile Thr Arg Phe Met Glu Gln Ala Ala

1 5 10 15

Thr Gly Arg Val Pro Ser Trp Val Pro Ile Val Ile Gly Val Val Met

20 25 30

Met Ser Phe Val Gly Ser Val Ala Leu Leu Pro Asp Met Gln Gly Leu

35 40 45

Ile Ser Ile Gly Thr Val Leu Leu Leu Leu Val Leu Asn Arg Phe Lys

50 55 60

Gly Arg Gly Ile Thr Ile Phe Leu Met Met Leu Ser Leu Leu Val Ser

65 70 75 80

Met Arg Tyr Val Val Trp Arg Leu Thr Ser Thr Ile Glu Phe His Gly

85 90 95

Trp Ile Gln Ser Ala Leu Ser Ile Leu Leu Leu Leu Ala Glu Val Tyr

100 105 110

Ala Leu Ser Thr Leu Cys Leu Ser Tyr Phe Gln Met Ala Trp Pro Leu

115 120 125

Gly Arg Lys Glu His Pro Leu Pro Glu Asp Thr Ser Ser Trp Pro His

130 135 140

Val Asp Ile Tyr Val Pro Ser Tyr Asn Glu Glu Leu Ser Leu Val Arg

145 150 155 160

Ser Thr Val Leu Gly Ala Leu Lys Leu Asp Trp Pro Glu Asp Lys Leu

165 170 175

His Val Tyr Ile Leu Asp Asp Gly Arg Arg Val Ala Phe Arg Asp Phe

180 185 190

Ala Leu Glu Ala Gly Ala Gly Tyr Ile Ile Arg Ser Gln Asn Asn His

195 200 205

Ala Lys Ala Gly Asn Leu Asn His Ala Leu Lys Ile Thr Asp Gly Gln

210 215 220

Phe Ala Val Ile Phe Asp Cys Asp His Val Pro Thr Arg Gly Phe Leu

225 230 235 240

Lys Arg Thr Ile Gly Trp Met Ile Ala Asp Pro Lys Leu Ala Leu Leu

245 250 255

Gln Thr Pro His His Phe Tyr Ala Pro Asp Pro Phe Gln Arg Asn Leu

260 265 270

Val Ala Gly Ala His Val Pro Pro Glu Gly Asn Met Phe Tyr Gly Leu

275 280 285

Val Gln Asp Gly Asn Asp Phe Trp Asp Ala Thr Phe Phe Cys Gly Ser

290 295 300

Cys Ala Val Ile Arg Arg Ser Ala Val Leu Gly Ile Gly Gly Phe Ala

305 310 315 320

Thr Glu Thr Val Thr Glu Asp Ala His Thr Ala Leu Lys Met Gln Arg

325 330 335

Arg Gly Trp Arg Thr Ala Tyr Leu Arg Glu Pro Leu Ala Gly Gly Leu

340 345 350

Ala Thr Glu Arg Leu Ile Leu His Ile Gly Gln Arg Val Arg Trp Ala

355 360 365

Arg Gly Met Leu Gln Ile Met Arg Arg Asp Asn Pro Leu Leu Gly Arg

370 375 380

Gly Leu Arg Trp Glu Gln Arg Leu Cys Tyr Leu Ser Ala Met Ser His

385 390 395 400

Phe Leu Phe Ala Ile Pro Arg Val Thr Phe Leu Val Ser Pro Leu Ala

405 410 415

Tyr Leu Phe Leu Gly Gln Asn Ile Ile Ala Ala Ser Pro Leu Ala Ile

420 425 430

Ser Val Tyr Ala Leu Pro His Ile Phe His Ser Ile Leu Thr Leu Ser

435 440 445

Arg Ile Glu Ser Arg Trp Arg Tyr Ser Phe Trp Ser Glu Ile Tyr Glu

450 455 460

Thr Ser Leu Ala Leu Phe Leu Val Arg Ile Thr Ile Val Thr Leu Leu

465 470 475 480

Gln Pro His Lys Gly Thr Phe Asn Val Thr Asp Lys Gly Gly Leu Leu

485 490 495

Glu Lys Ser Tyr Phe Asp Val Gly Ala Val Tyr Pro Asn Val Ile Leu

500 505 510

Ala Val Ile Leu Phe Ala Ala Phe Leu Arg Gly Ile Phe Gly Ile Val

515 520 525

Trp Gln Phe His Asp Arg Leu Ala Leu Gln Ser Phe Ala Leu Asn Thr

530 535 540

Leu Trp Val Val Ile Ser Leu Ile Ile Val Leu Ala Ser Ile Ala Val

545 550 555 560

Gly Arg Glu Thr Arg Gln Thr Arg Ala Ala Pro Arg Ile Ala Val Ala

565 570 575

Leu Pro Val Arg Ile Thr Asp Met Glu Gly Arg Ser Phe Ala Gly His

580 585 590

Thr Arg Asp Ile Ser Leu Gly Gly Leu Gly Val Asp Leu His Trp Pro

595 600 605

Ala Asp Val Ala Arg Pro Asp Arg Val Met Met Glu Tyr Val Asn Glu

610 615 620

Arg Asp Gly Ile His Ala Thr Val Pro Ala Thr Val Leu Ala Leu Asp

625 630 635 640

Glu Arg Ser Met Arg Leu Gln Trp Glu Arg Arg Asp Leu Glu Asp Glu

645 650 655

Ser Gln Ile Val Asp Met Val Phe Gly Arg Asn Asp Ala Trp Ala Asn

660 665 670

Trp Ala Asp Phe Glu Pro Asp Arg Pro Leu Arg Ser Ile Ala Met Val

675 680 685

Leu Arg Ser Ile Gly Gly Leu Phe Lys Trp Gln Ala Arg Glu Ile Pro

690 695 700

Arg His Val Ala Asp Asp Glu Glu Ala Lys Ala Pro Val Val Glu Ala

705 710 715 720

Lys Leu Glu Lys Gln Ser Leu Val Leu Lys Pro Val Arg Arg Ser Ala

725 730 735

Arg Asn Gly Ala Ala Ala Ser Ala Val Leu Leu Val Ala Leu Ala Ala

740 745 750

Leu Ser Pro Val Ala Met Ala Gln Val Gln Pro Ala Ala Ser Ala Ser

755 760 765

Val Pro Asp Gln Thr Gly Val Ser Ala Glu Thr Pro Phe Gly Asp Ser

770 775 780

Asn Thr Gly Thr Val Pro Asp Met Ile Pro Val Ile Asp Gln Ala Ala

785 790 795 800

Ala Asp Arg Ile Ser Asp Ser Glu Val Thr Arg Thr Leu Ser Phe Arg

805 810 815

Asn Leu Gly Ala Thr Ser Gly Pro Leu Thr Leu Arg Gly Tyr Ser Pro

820 825 830

Leu Gln Gly Leu Asp Val Ile Val Pro Ala Asn Arg Val Val Thr His

835 840 845

Ala Arg Leu Ser Ile Ser Gly Ala Leu Ser Pro Ser Leu Leu Pro Glu

850 855 860

Ala Ser Ala Val Thr Val Thr Leu Asn Glu Gln Tyr Ile Gly Thr Ile

865 870 875 880

Lys Val Asp Pro Glu His Pro Gln Phe Gly Pro Leu Thr Phe Asp Ile

885 890 895

Asp Pro Leu Tyr Phe Thr Gly Asp Asn Lys Leu Asn Phe Arg Phe Ala

900 905 910

Gly Glu Tyr Arg Arg Asp Cys Lys Glu Val Val Pro Ile Phe Arg Thr

915 920 925

Gly Arg

930

<210> 13

<211> 849

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1007 (BcsAB II)

<400> 13

atgaccgacc accgcgcgct gtccatcggc agccttacgg ttggcaacca gtcccccttc 60

acgctgattg caggcccgtg ccagatcgaa tcagcaagcc acgcgatgga ggtggcggac 120

gcgctgcatg gcatcgccac gcgactgggg atcgggctga tctacaaaag ctcgttcgac 180

aaggccaacc gcacctccat caacggcgcg cgcggcgtgg gcatggcgca ggggctggac 240

atactgggtc aggtgcgcgc ccgcttcggc atgccggtgc tgaccgacgt gcacctgccc 300

gaccagtgcg ccgcggtggc cgagacggtg gatgtgctcc agatccccgc cttcctgtgc 360

cgccagaccg accttctgct ggcggcgggc cagacggggg cggccatcaa cataaaaaag 420

gggcagttcc ttgccccgtg ggacatggcg aacgtggccg ccaaggtggc ttccaccggc 480

aatgagcgca tcatgctgtg cgagcgtggc acgtcatttg gctacaacac gctggtcaat 540

gacatgcgcg gcctgccgat catggcgggc acggggtatc cggtcatctt tgacgccacc 600

cattccgtcc agcagccggg ggggctgggc acatcctcgg gcgggcagcg ggtattcgcc 660

cccatcctgg cgcgcgcggc gctggccgta ggggttgcgg gggtgttcat tgaaacccat 720

cccgaccccg atacagcccc cagcgatggt acgaccatgc tgccgctggc ctgtatggaa 780

gacctgctga ccaccctgct gcgctatgac cggctgacca agcagaaccc gccagccgag 840

atcgtctga 849

<210> 14

<211> 282

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_1007 (BcsAB II)

<400> 14

Met Thr Asp His Arg Ala Leu Ser Ile Gly Ser Leu Thr Val Gly Asn

1 5 10 15

Gln Ser Pro Phe Thr Leu Ile Ala Gly Pro Cys Gln Ile Glu Ser Ala

20 25 30

Ser His Ala Met Glu Val Ala Asp Ala Leu His Gly Ile Ala Thr Arg

35 40 45

Leu Gly Ile Gly Leu Ile Tyr Lys Ser Ser Phe Asp Lys Ala Asn Arg

50 55 60

Thr Ser Ile Asn Gly Ala Arg Gly Val Gly Met Ala Gln Gly Leu Asp

65 70 75 80

Ile Leu Gly Gln Val Arg Ala Arg Phe Gly Met Pro Val Leu Thr Asp

85 90 95

Val His Leu Pro Asp Gln Cys Ala Ala Val Ala Glu Thr Val Asp Val

100 105 110

Leu Gln Ile Pro Ala Phe Leu Cys Arg Gln Thr Asp Leu Leu Leu Ala

115 120 125

Ala Gly Gln Thr Gly Ala Ala Ile Asn Ile Lys Lys Gly Gln Phe Leu

130 135 140

Ala Pro Trp Asp Met Ala Asn Val Ala Ala Lys Val Ala Ser Thr Gly

145 150 155 160

Asn Glu Arg Ile Met Leu Cys Glu Arg Gly Thr Ser Phe Gly Tyr Asn

165 170 175

Thr Leu Val Asn Asp Met Arg Gly Leu Pro Ile Met Ala Gly Thr Gly

180 185 190

Tyr Pro Val Ile Phe Asp Ala Thr His Ser Val Gln Gln Pro Gly Gly

195 200 205

Leu Gly Thr Ser Ser Gly Gly Gln Arg Val Phe Ala Pro Ile Leu Ala

210 215 220

Arg Ala Ala Leu Ala Val Gly Val Ala Gly Val Phe Ile Glu Thr His

225 230 235 240

Pro Asp Pro Asp Thr Ala Pro Ser Asp Gly Thr Thr Met Leu Pro Leu

245 250 255

Ala Cys Met Glu Asp Leu Leu Thr Thr Leu Leu Arg Tyr Asp Arg Leu

260 265 270

Thr Lys Gln Asn Pro Pro Ala Glu Ile Val

275 280

<210> 15

<211> 669

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3007 (BcsX)

<400> 15

atgaatgctc ttcttgctgg cctgacactt ctgattatcg gtgatagtca cgtaaccttc 60

aaagacaccc tgctttcggt cctgcctgat gaatttacca gacagggcgc aagggtcgtg 120

acctatggtg tgtgttcctc cactgcggcg gactgggttg tgcctaatcc caataacgga 180

tgcggggcgg ccgaacgcat cggcaccgcc ccgatcggcg cgcccgacat gaagccggcc 240

tcgccgccgc cggtggccga actgattgcg aaatggcacc ccgacgcggt catggtcatc 300

ctgggggata ccatggccgc ctatgggcag ggcgcggtct cgaaggactg ggtggacgag 360

caggtcaagt ccctgaccta tacgattggc aggaccgcgt gcatctgggt cgggccgacc 420

tgggggcagt tcagcccgcg ttatggcaag accgaccagc gcgcgatgga aatggcaagc 480

ttcctgaagg gggaggtggc gccatgcacc tatatcgatg gcacggcgct gctcaggcag 540

ggaagcgtca gtaccaccga tggcattcac gcgacaccgg aaagctaccg ggcctggggc 600

aatgccatcg tgcaggcggc gctgccggaa ctggagaagc tgaagtctcc cgccgcggcg 660

ggacagtag 669

<210> 16

<211> 222

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3007 (BcsX)

<400> 16

Met Asn Ala Leu Leu Ala Gly Leu Thr Leu Leu Ile Ile Gly Asp Ser

1 5 10 15

His Val Thr Phe Lys Asp Thr Leu Leu Ser Val Leu Pro Asp Glu Phe

20 25 30

Thr Arg Gln Gly Ala Arg Val Val Thr Tyr Gly Val Cys Ser Ser Thr

35 40 45

Ala Ala Asp Trp Val Val Pro Asn Pro Asn Asn Gly Cys Gly Ala Ala

50 55 60

Glu Arg Ile Gly Thr Ala Pro Ile Gly Ala Pro Asp Met Lys Pro Ala

65 70 75 80

Ser Pro Pro Pro Val Ala Glu Leu Ile Ala Lys Trp His Pro Asp Ala

85 90 95

Val Met Val Ile Leu Gly Asp Thr Met Ala Ala Tyr Gly Gln Gly Ala

100 105 110

Val Ser Lys Asp Trp Val Asp Glu Gln Val Lys Ser Leu Thr Tyr Thr

115 120 125

Ile Gly Arg Thr Ala Cys Ile Trp Val Gly Pro Thr Trp Gly Gln Phe

130 135 140

Ser Pro Arg Tyr Gly Lys Thr Asp Gln Arg Ala Met Glu Met Ala Ser

145 150 155 160

Phe Leu Lys Gly Glu Val Ala Pro Cys Thr Tyr Ile Asp Gly Thr Ala

165 170 175

Leu Leu Arg Gln Gly Ser Val Ser Thr Thr Asp Gly Ile His Ala Thr

180 185 190

Pro Glu Ser Tyr Arg Ala Trp Gly Asn Ala Ile Val Gln Ala Ala Leu

195 200 205

Pro Glu Leu Glu Lys Leu Lys Ser Pro Ala Ala Ala Gly Gln

210 215 220

<210> 17

<211> 1113

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3008 (BcsY)

<400> 17

atgttcggaa aggacttctt tccccaaaac cgtcggcgcg atatcgacgg gctgcgtggt 60

cttgccgttg cccttgttgt cctgttccat gcgggctggc tgaagggggg ctttgtcggg 120

gttgacgtct ttgtcgtgat ctcgggctat ttcatggatc gttcggcgct catgcagcat 180

ccgttccagc caatgcgctt tgtctgccgc aggctttacc gcctgcttcc cgcgctgctg 240

tgcatgatcg cgctggtctc ggccggcatg ctgtggtggg tgctgcagag tgaccgggcc 300

gatatcgccg ataacggcgc ctatgccctg gtctacctgt ccaatatctg ggcatcgggg 360

catgtcggct atttccaggg gcagagcatt gcctacccct tcctgcatac atggtcgctt 420

tcgctggaaa tgcagttcta tgccatcatc ttcttgatgg ccctgctgct gccgctgaca 480

cggcaccgca ggctggtact gtcgggcatc tgcgtggcgt cgtttgcctt ttgcatcagc 540

tgcgacatcc ggggggatac gcaggcctat tacaacatct ttgcccggct gtggcagttc 600

tcgcttggca cgatgatctg gatgttcccg cgcccccgac tgtcggcggt ggcggcgaat 660

accatccatg ccggggccgt tggggtgatt gtgggcgcgg ccctgttcta tacgctccgc 720

tttgcctgtc cgtccagcat ggcggttttt ccctgtgtgg ccgttgcggc catcatcatg 780

ctgccccaga cgcaggcggg caggctgtgc ctggtgccgc tttccccgct gggggtcatt 840

tcctactcgg tctatctttg gcactggccg ggtattgtgg tggcaaacta ccttctgttc 900

tttcaggtgc atggtctggt catggcggcg gttctgggca tcgtgctcgt ggtcagcctg 960

ttcagttacc tgctggtgga acgtaccggc ctgaattacg aggacagggt cgtccccgcc 1020

gtccgcaacc gtggggcggc cctgctggtg gggtcatgca tggtgctggc catggtcctg 1080

ttctacgtct cccgtgtgtc ccgtgttcat tag 1113

<210> 18

<211> 370

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3008 (BcsY)

<400> 18

Met Phe Gly Lys Asp Phe Phe Pro Gln Asn Arg Arg Arg Asp Ile Asp

1 5 10 15

Gly Leu Arg Gly Leu Ala Val Ala Leu Val Val Leu Phe His Ala Gly

20 25 30

Trp Leu Lys Gly Gly Phe Val Gly Val Asp Val Phe Val Val Ile Ser

35 40 45

Gly Tyr Phe Met Asp Arg Ser Ala Leu Met Gln His Pro Phe Gln Pro

50 55 60

Met Arg Phe Val Cys Arg Arg Leu Tyr Arg Leu Leu Pro Ala Leu Leu

65 70 75 80

Cys Met Ile Ala Leu Val Ser Ala Gly Met Leu Trp Trp Val Leu Gln

85 90 95

Ser Asp Arg Ala Asp Ile Ala Asp Asn Gly Ala Tyr Ala Leu Val Tyr

100 105 110

Leu Ser Asn Ile Trp Ala Ser Gly His Val Gly Tyr Phe Gln Gly Gln

115 120 125

Ser Ile Ala Tyr Pro Phe Leu His Thr Trp Ser Leu Ser Leu Glu Met

130 135 140

Gln Phe Tyr Ala Ile Ile Phe Leu Met Ala Leu Leu Leu Pro Leu Thr

145 150 155 160

Arg His Arg Arg Leu Val Leu Ser Gly Ile Cys Val Ala Ser Phe Ala

165 170 175

Phe Cys Ile Ser Cys Asp Ile Arg Gly Asp Thr Gln Ala Tyr Tyr Asn

180 185 190

Ile Phe Ala Arg Leu Trp Gln Phe Ser Leu Gly Thr Met Ile Trp Met

195 200 205

Phe Pro Arg Pro Arg Leu Ser Ala Val Ala Ala Asn Thr Ile His Ala

210 215 220

Gly Ala Val Gly Val Ile Val Gly Ala Ala Leu Phe Tyr Thr Leu Arg

225 230 235 240

Phe Ala Cys Pro Ser Ser Met Ala Val Phe Pro Cys Val Ala Val Ala

245 250 255

Ala Ile Ile Met Leu Pro Gln Thr Gln Ala Gly Arg Leu Cys Leu Val

260 265 270

Pro Leu Ser Pro Leu Gly Val Ile Ser Tyr Ser Val Tyr Leu Trp His

275 280 285

Trp Pro Gly Ile Val Val Ala Asn Tyr Leu Leu Phe Phe Gln Val His

290 295 300

Gly Leu Val Met Ala Ala Val Leu Gly Ile Val Leu Val Val Ser Leu

305 310 315 320

Phe Ser Tyr Leu Leu Val Glu Arg Thr Gly Leu Asn Tyr Glu Asp Arg

325 330 335

Val Val Pro Ala Val Arg Asn Arg Gly Ala Ala Leu Leu Val Gly Ser

340 345 350

Cys Met Val Leu Ala Met Val Leu Phe Tyr Val Ser Arg Val Ser Arg

355 360 365

Val His

370

<210> 19

<211> 3879

<212> DNA

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3009 (BcsC II)

<400> 19

atgcgccccg ccgcctgccg gccgtcatcg gcatggctgg ccgggggtgg atggaaaatc 60

atctgtggca tggttgccgg tgtcatcatg tccggggaga tcgcgcaggc ggaacccgcc 120

tggacggcag gaccggacac cgcggcgtcc cccgccggtg gcagcaccca gattaccgtg 180

acccccgatg caggccagaa cgcggccaac aacgcgcatc tggcgcatgc cgcggccgtg 240

ctggaactgc tgctgaacca gggatattac tggctggggc agcacaacct gcccaaggca 300

cgcgagacga tccagcgtgc gttgtccatc gagccggata ataacgaggc ccttttcctg 360

ctgggtcgcc tgcagatggc ggaaggccag acgaagcttg ccgccgccac actgggcagg 420

ctgatccaga acgggaatgc accgggtctg gtggctgatc tcagggccca gatccatgcc 480

ggtccgatag accccagggg gctggcggaa gcccgcgccc ttgcggccga gggcaagatg 540

atgcccgcga tgttcaaata ccgcgccctg ttcaagaacg gtgacccgcc gcccgacctg 600

gccatggaat attaccgcgt tctgggggcc accacccttg gctatcagga agccgccacc 660

aggcttgccg cctgggtggc gcgcaacccg cgtgacctgg acgcaaaact ttcgctggac 720

cggatcctga cctatcatgt cacctcgcgt gacgaggggc tggatggcct gcgccagctt 780

gcccgttcca atgcttccgc cgcgatacgt gacggggcca ttgccgcctg gcgtgacgcc 840

ctgctgtggg agccggtcac gggacccacc atcgcactgt ataacgagtg gcttgacctg 900

catccgggtg atgcggaaat cattgaccgc cggcacaagg cgcaggatgc gcagaatatc 960

attgatggcg cgaattaccg ccagcagggt ttcgtgctgc tgtcgcgcag gaatatcgaa 1020

ggggccgccg acctgttcca tcgcgccctg gccatcaacg cccatgacgc ggactcgctt 1080

ggtggcatgg cgcttgtggc gcaggcccgc cagcagcccg ccctggcacg gcgctatttc 1140

cagcaggcca tacaggcgga cccggattcc gcagcccact ggcgcgcggc cctgaaggcg 1200

atggaggcag gcggtggtgg cgggatggac ccgctggtgg cacggatcat acaggccatc 1260

aattccggac attatgatgc ggcacagtcc gacctggcgc tgctgggccg ccggggcaac 1320

acgatcacgc tgcaggcgct gcagggcatg ctggcgcgca ggcagggcca tatggaggaa 1380

gccgagcgcc tgtatcgtga gatcctgcgc cgcgcgccgg gcaatgccga tgcgctgttc 1440

aatctgggcg ggatcctgat tgaaaccgga cgcgaggccg aagcgcagga tatcatcgcg 1500

cgtcttgcgc acatccgtcc cgaccttgcg cggcatctgg aagtggccgg gctttcagcc 1560

cggtcggacc ggacacgcaa taatgacgaa aagctttcat tgctgaaccg ggccatggcc 1620

atggccccca cggacccgtg gatacggctc aagctcgcgc gcgcgctgga tgaagcgggc 1680

aaccatgcgc aggcccaggc catcatggat ggcgtgacca gtggccgcgc cgtcaccccc 1740

gatgacctgc aggccgcgat cctctatgca atgggccgcc atgacatggc cagggcggaa 1800

cagcttctgg cgcggctgcc ccccggtatc gaaagccccg gcatggcccg cgtggccgag 1860

cagatcgaac tggtccggcg catacaggaa ctcaaccgcg tgccgcgtgc gcccaacgcg 1920

ctccttgtgg cgctggccga ccggcctgac cccacgggtg aacgtggcat gcgtatcgcc 1980

aacgcgctgc tcgaccgcca tgccccacag gatgcccagc aggtactggc aacggaagaa 2040

cgtctgaccc agccgcccca gccatcccag ctccttgcct atgcgggtgt gtacctgcgg 2100

ctgcattccg ctgttgatgc aacgcgatgc ctcaatgcct ttgatgcgat ggcccgggcc 2160

cggcccaccg acatcacggc ggaccagcag gagatacgca accagatcgc cattggcctt 2220

gccatcatga cggcggacgg gttcaaccgt tatggccaga cggcgcgggc gtatcaggta 2280

ctggcgccgg tgctgcaggc acatccggat tccgccgagg cgcatctggc catggggcgc 2340

gtctaccaga cccgcaacat ggcccgtcgc gcactggaag aggaccagat cgccctgcgc 2400

ctgaaaccgc acaacatata cgcccttgcc gccgccgcgc gtgacgcggg gggactgcac 2460

cagatggcag aggcaaaaga ctattccacc cggctggcac atgaagaccc cgacggcccg 2520

atgagctggg aagtccgttc cgatatcgaa cggatagagg gcaacacacg cgcgcagctg 2580

gtggatgtgg agcatgcgcg ccacgcgcag tgcacgcttg atggtgaagg gacatgcgac 2640

gggcagcatg aaagcttcct gccggactat cgctggcctg agatcgacag caactacatc 2700

aacctgcatg gcgcgaccct gcctgcaacc tatcactaca ttcccgaaga tgacgggccg 2760

gaggcaatgg accgccagat cgtctatctg cgggattccg tatcgcccca gatcgatgcc 2820

aattcctacg tgcgcagccg tacgggcacc gcggggctgg ggcagctgac ggaattcgcc 2880

gtgccgatta cgggcacgct gccgtttgaa tcctgggagc acaggctgtc cttctcgatc 2940

gtgcccacgc tcctgttcac cggcaatccg cttgccaatc cctattcggc gcatgaattc 3000

ggtacctatg ccatcaatgg ggcgctgccc ggctcatccc accattatta cacgcagggt 3060

gtggggctga gcctgaatta cgtcaatcac tggttctcgg cggatgtggg gtcgtcgcca 3120

ctgggctttc ccattaccaa tgtggtgggc ggggttgaat tcgcgccacg cctgacccgc 3180

aacctcggcc tgcgcataag tggtggccgc cgcatggtga cggatagcga actgtcctat 3240

gccgggatgc gcgatccggg caccggcagg ctatggggcg gtgtgacacg catgttcggc 3300

catggcgcgc ttgaatggtc ggagccgaca tggaacctgt acgcgggcgg tggttttgcc 3360

tatctgggtg gcacccatgt cgttgacaat accgaagtgg aggccggcgc gggtggcagt 3420

gccacggtat ggcagtcgca tgacaggcag tggctgcggg tcgggcttga cctgatgtat 3480

ttcggctaca agcgcgatac ctattccttt acatgggggc aggggggata cttctcgccg 3540

cagcagtatt acggcgccat gataccggtc gaatggtcgg gacatgaccg gcggtggaca 3600

tggttcctgc gtggtgaagc gggtttccag cactatcaca gcaatggcgc gccttattac 3660

ccgacggatt ccgccctgca ggcgctttct gtggcgcacc agcccgatta ttatggtgat 3720

gagggggaaa gcggccttgc ggggaacata cgtggtcgtg ttgtctatca gttcacccac 3780

cgcctgcgtc tgggcatgga aggtggttac agccgggcag gaaactggtc ggaaaccagc 3840

ggcatgtgga tggcacacta tacttttgat ggccagtaa 3879

<210> 20

<211> 1292

<212> PRT

<213> artificial sequence

<220>

<223> Komagataeibacter rhaeticus KOSS15_3009 (BcsC II)

<400> 20

Met Arg Pro Ala Ala Cys Arg Pro Ser Ser Ala Trp Leu Ala Gly Gly

1 5 10 15

Gly Trp Lys Ile Ile Cys Gly Met Val Ala Gly Val Ile Met Ser Gly

20 25 30

Glu Ile Ala Gln Ala Glu Pro Ala Trp Thr Ala Gly Pro Asp Thr Ala

35 40 45

Ala Ser Pro Ala Gly Gly Ser Thr Gln Ile Thr Val Thr Pro Asp Ala

50 55 60

Gly Gln Asn Ala Ala Asn Asn Ala His Leu Ala His Ala Ala Ala Val

65 70 75 80

Leu Glu Leu Leu Leu Asn Gln Gly Tyr Tyr Trp Leu Gly Gln His Asn

85 90 95

Leu Pro Lys Ala Arg Glu Thr Ile Gln Arg Ala Leu Ser Ile Glu Pro

100 105 110

Asp Asn Asn Glu Ala Leu Phe Leu Leu Gly Arg Leu Gln Met Ala Glu

115 120 125

Gly Gln Thr Lys Leu Ala Ala Ala Thr Leu Gly Arg Leu Ile Gln Asn

130 135 140

Gly Asn Ala Pro Gly Leu Val Ala Asp Leu Arg Ala Gln Ile His Ala

145 150 155 160

Gly Pro Ile Asp Pro Arg Gly Leu Ala Glu Ala Arg Ala Leu Ala Ala

165 170 175

Glu Gly Lys Met Met Pro Ala Met Phe Lys Tyr Arg Ala Leu Phe Lys

180 185 190

Asn Gly Asp Pro Pro Pro Asp Leu Ala Met Glu Tyr Tyr Arg Val Leu

195 200 205

Gly Ala Thr Thr Leu Gly Tyr Gln Glu Ala Ala Thr Arg Leu Ala Ala

210 215 220

Trp Val Ala Arg Asn Pro Arg Asp Leu Asp Ala Lys Leu Ser Leu Asp

225 230 235 240

Arg Ile Leu Thr Tyr His Val Thr Ser Arg Asp Glu Gly Leu Asp Gly

245 250 255

Leu Arg Gln Leu Ala Arg Ser Asn Ala Ser Ala Ala Ile Arg Asp Gly

260 265 270

Ala Ile Ala Ala Trp Arg Asp Ala Leu Leu Trp Glu Pro Val Thr Gly

275 280 285

Pro Thr Ile Ala Leu Tyr Asn Glu Trp Leu Asp Leu His Pro Gly Asp

290 295 300

Ala Glu Ile Ile Asp Arg Arg His Lys Ala Gln Asp Ala Gln Asn Ile

305 310 315 320

Ile Asp Gly Ala Asn Tyr Arg Gln Gln Gly Phe Val Leu Leu Ser Arg

325 330 335

Arg Asn Ile Glu Gly Ala Ala Asp Leu Phe His Arg Ala Leu Ala Ile

340 345 350

Asn Ala His Asp Ala Asp Ser Leu Gly Gly Met Ala Leu Val Ala Gln

355 360 365

Ala Arg Gln Gln Pro Ala Leu Ala Arg Arg Tyr Phe Gln Gln Ala Ile

370 375 380

Gln Ala Asp Pro Asp Ser Ala Ala His Trp Arg Ala Ala Leu Lys Ala

385 390 395 400

Met Glu Ala Gly Gly Gly Gly Gly Met Asp Pro Leu Val Ala Arg Ile

405 410 415

Ile Gln Ala Ile Asn Ser Gly His Tyr Asp Ala Ala Gln Ser Asp Leu

420 425 430

Ala Leu Leu Gly Arg Arg Gly Asn Thr Ile Thr Leu Gln Ala Leu Gln

435 440 445

Gly Met Leu Ala Arg Arg Gln Gly His Met Glu Glu Ala Glu Arg Leu

450 455 460

Tyr Arg Glu Ile Leu Arg Arg Ala Pro Gly Asn Ala Asp Ala Leu Phe

465 470 475 480

Asn Leu Gly Gly Ile Leu Ile Glu Thr Gly Arg Glu Ala Glu Ala Gln

485 490 495

Asp Ile Ile Ala Arg Leu Ala His Ile Arg Pro Asp Leu Ala Arg His

500 505 510

Leu Glu Val Ala Gly Leu Ser Ala Arg Ser Asp Arg Thr Arg Asn Asn

515 520 525

Asp Glu Lys Leu Ser Leu Leu Asn Arg Ala Met Ala Met Ala Pro Thr

530 535 540

Asp Pro Trp Ile Arg Leu Lys Leu Ala Arg Ala Leu Asp Glu Ala Gly

545 550 555 560

Asn His Ala Gln Ala Gln Ala Ile Met Asp Gly Val Thr Ser Gly Arg

565 570 575

Ala Val Thr Pro Asp Asp Leu Gln Ala Ala Ile Leu Tyr Ala Met Gly

580 585 590

Arg His Asp Met Ala Arg Ala Glu Gln Leu Leu Ala Arg Leu Pro Pro

595 600 605

Gly Ile Glu Ser Pro Gly Met Ala Arg Val Ala Glu Gln Ile Glu Leu

610 615 620

Val Arg Arg Ile Gln Glu Leu Asn Arg Val Pro Arg Ala Pro Asn Ala

625 630 635 640

Leu Leu Val Ala Leu Ala Asp Arg Pro Asp Pro Thr Gly Glu Arg Gly

645 650 655

Met Arg Ile Ala Asn Ala Leu Leu Asp Arg His Ala Pro Gln Asp Ala

660 665 670

Gln Gln Val Leu Ala Thr Glu Glu Arg Leu Thr Gln Pro Pro Gln Pro

675 680 685

Ser Gln Leu Leu Ala Tyr Ala Gly Val Tyr Leu Arg Leu His Ser Ala

690 695 700

Val Asp Ala Thr Arg Cys Leu Asn Ala Phe Asp Ala Met Ala Arg Ala

705 710 715 720

Arg Pro Thr Asp Ile Thr Ala Asp Gln Gln Glu Ile Arg Asn Gln Ile

725 730 735

Ala Ile Gly Leu Ala Ile Met Thr Ala Asp Gly Phe Asn Arg Tyr Gly

740 745 750

Gln Thr Ala Arg Ala Tyr Gln Val Leu Ala Pro Val Leu Gln Ala His

755 760 765

Pro Asp Ser Ala Glu Ala His Leu Ala Met Gly Arg Val Tyr Gln Thr

770 775 780

Arg Asn Met Ala Arg Arg Ala Leu Glu Glu Asp Gln Ile Ala Leu Arg

785 790 795 800

Leu Lys Pro His Asn Ile Tyr Ala Leu Ala Ala Ala Ala Arg Asp Ala

805 810 815

Gly Gly Leu His Gln Met Ala Glu Ala Lys Asp Tyr Ser Thr Arg Leu

820 825 830

Ala His Glu Asp Pro Asp Gly Pro Met Ser Trp Glu Val Arg Ser Asp

835 840 845

Ile Glu Arg Ile Glu Gly Asn Thr Arg Ala Gln Leu Val Asp Val Glu

850 855 860

His Ala Arg His Ala Gln Cys Thr Leu Asp Gly Glu Gly Thr Cys Asp

865 870 875 880

Gly Gln His Glu Ser Phe Leu Pro Asp Tyr Arg Trp Pro Glu Ile Asp

885 890 895

Ser Asn Tyr Ile Asn Leu His Gly Ala Thr Leu Pro Ala Thr Tyr His

900 905 910

Tyr Ile Pro Glu Asp Asp Gly Pro Glu Ala Met Asp Arg Gln Ile Val

915 920 925

Tyr Leu Arg Asp Ser Val Ser Pro Gln Ile Asp Ala Asn Ser Tyr Val

930 935 940

Arg Ser Arg Thr Gly Thr Ala Gly Leu Gly Gln Leu Thr Glu Phe Ala

945 950 955 960

Val Pro Ile Thr Gly Thr Leu Pro Phe Glu Ser Trp Glu His Arg Leu

965 970 975

Ser Phe Ser Ile Val Pro Thr Leu Leu Phe Thr Gly Asn Pro Leu Ala

980 985 990

Asn Pro Tyr Ser Ala His Glu Phe Gly Thr Tyr Ala Ile Asn Gly Ala

995 1000 1005

Leu Pro Gly Ser Ser His His Tyr Tyr Thr Gln Gly Val Gly Leu Ser

1010 1015 1020

Leu Asn Tyr Val Asn His Trp Phe Ser Ala Asp Val Gly Ser Ser Pro

1025 1030 1035 1040

Leu Gly Phe Pro Ile Thr Asn Val Val Gly Gly Val Glu Phe Ala Pro

1045 1050 1055

Arg Leu Thr Arg Asn Leu Gly Leu Arg Ile Ser Gly Gly Arg Arg Met

1060 1065 1070

Val Thr Asp Ser Glu Leu Ser Tyr Ala Gly Met Arg Asp Pro Gly Thr

1075 1080 1085

Gly Arg Leu Trp Gly Gly Val Thr Arg Met Phe Gly His Gly Ala Leu

1090 1095 1100

Glu Trp Ser Glu Pro Thr Trp Asn Leu Tyr Ala Gly Gly Gly Phe Ala

1105 1110 1115 1120

Tyr Leu Gly Gly Thr His Val Val Asp Asn Thr Glu Val Glu Ala Gly

1125 1130 1135

Ala Gly Gly Ser Ala Thr Val Trp Gln Ser His Asp Arg Gln Trp Leu

1140 1145 1150

Arg Val Gly Leu Asp Leu Met Tyr Phe Gly Tyr Lys Arg Asp Thr Tyr

1155 1160 1165

Ser Phe Thr Trp Gly Gln Gly Gly Tyr Phe Ser Pro Gln Gln Tyr Tyr

1170 1175 1180

Gly Ala Met Ile Pro Val Glu Trp Ser Gly His Asp Arg Arg Trp Thr

1185 1190 1195 1200

Trp Phe Leu Arg Gly Glu Ala Gly Phe Gln His Tyr His Ser Asn Gly

1205 1210 1215

Ala Pro Tyr Tyr Pro Thr Asp Ser Ala Leu Gln Ala Leu Ser Val Ala

1220 1225 1230

His Gln Pro Asp Tyr Tyr Gly Asp Glu Gly Glu Ser Gly Leu Ala Gly

1235 1240 1245

Asn Ile Arg Gly Arg Val Val Tyr Gln Phe Thr His Arg Leu Arg Leu

1250 1255 1260

Gly Met Glu Gly Gly Tyr Ser Arg Ala Gly Asn Trp Ser Glu Thr Ser

1265 1270 1275 1280

Gly Met Trp Met Ala His Tyr Thr Phe Asp Gly Gln

1285 1290

<210> 21

<211> 3558

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2950 (BcsA I)

<400> 21

atggacgcca tcggccttgc caatcgcagc caggcgcagg catcggaccc gcaggcatcg 60

gtgtttgtct cggcctcggc gggtagtggc aagaccaagc tgctgatcga ccggctgctg 120

cgcctcatgc tgccgcgtga cgtgcccgac cgtgacggcg tgcccacccg tgtcgcgggc 180

agcgaccccg gtcgcatcct gtgcctgacc tttaccaagg cggcggcagc cgaaatgtcg 240

atccgcctgc aggaccggct gggccagtgg gtcatgctgc ctgatgcggc gctggatggg 300

gaacttgccc gcctgtcggt gccggtggat gagcgcacgc gcgaggtggc gcgtgaactg 360

tttgcccgcg tgcttgatct gcccggcggc atgcgcattg gcaccatcca tgccttctgc 420

cagtcgctgc tgcggcgctt ccccattgaa gccgcgatca gcccgcattt caccctgatc 480

gaggaaaccg atgcccgcct ggcccagcgc gcggcggtgg aggacgtggt gggcgcgggc 540

ggccccgccg tggggctgct ggcgggtcag atcggcatgg gtgactttac gtccctgatc 600

gcgcggttgc aggcgcatgc ccgccgcctg ctgccggtgg cgcggcagtg ggtgcgcgac 660

cgggccagtg tcgaggctgc cctgatgcgc ctgctgggca taagcgggcg cagcgtggag 720

gaggtgctgc gccaggcctg cgccaccatc cccgatgagg acgccctgcg cgacggcctg 780

cgttacgcgg cgcaggaagg atcgcccacc gtcaggacgc aggccgaaat catgctggcc 840

tggctggggc aggatgcggc ggcccgcgcg gcacaatggc ccgtgtggcg caaggcgctg 900

ctgaaaaacg atggcgcgcc ccgtcagcgc ggcgggctga acgccaaact tgccgccgcg 960

cgcccggtgc tggtcgaaca gctgctggcg gaggcggcgc gcatcatcgc cattgagcag 1020

cagatgcgtg cgatcacggt gtttgaactg acctgcgcgc tgctctcggt cgcgcagccg 1080

gtgctggagc gttacgccac gcgcaagagc gcgcagggca tggttgatta tgatgacctg 1140

atcgaccgca cgctcgaact gctgcgtgac cccggtgcgg cgtgggtact ctacaagctc 1200

gacggcggca tcgaccacct tctgcttgat gaggtgcagg atacatcatt cgaccagtgg 1260

gccatcgcgg gggggctgac ggcggagttc ttcgcaggcg aaggcacgca tgatgacgag 1320

ggcacgccgc gcaccatctt cgcggtgggg gactacaagc agtcgatcta ttcgtttcag 1380

ggggccgacc ccgaggcctt ccgcgcgtgg cggcagcgct ttcgccacag tgcggcggca 1440

gccggggccc tgtggcgcga accggccctg accgtttcct tccgctccac cgccccggtg 1500

ctcaaactgg tggacagcgt gttcgccaac gccgccgccg cccggggcgt ggccgagccc 1560

gatggcacca ttccccccca tgtcaccgcc cgccccggcc agggcggccg cgtcgagatc 1620

tggccccccg tgccggtgga ggaggatggc gaggcagtca gcccctggca ggcccccagc 1680

cagaacgcgg gccagtccac tgcccagcag cgcctggccg atacgctggc cgcctggatt 1740

gcagccgaac tgcgcaggcc cccggcaccg ggcgaggccc cgcttgcgcc gggtgacgtg 1800

ctgattctcg tgccgcgtcg ctcggccttc gcccgcgccc tgatccgcgc gctgaaaacg 1860

aacgacgtgc cggtggccac cctcgtgcgc accgtgctga ccgaccagct tgccgtgcag 1920

gatctcatgg cgctgtgcgc gtgtctgctc ctgccgcagg atgacctgac gctggcctgc 1980

gtgctgacct cgcccattgg cgggctggat gatgagtcgc tcatgcatct tgcaacgggc 2040

cgcgatggca aaccgctctg ggccgtgctg cgcgcgcgcc ataccgagcg cccggactgg 2100

gcggcggcat ggcatatcct caacacgctg ttccggcagg tggactacgc aaccccctac 2160

cagcttctgg ccgaggccct tgggccgctg ggggcgcgca cgcggctgct ggcccgcctt 2220

gggccggagg cggtggagcc ggtggatgaa ctgctctccg ccgcgctgcg ctttgaggag 2280

gcgcatgcca tctcgctgca gggcttcctg cactggctca atgcctcgga tgagaccgtg 2340

cgccatgagc ctgacgcctc ggccaacatg gtgcgcgtca tgaccgcgca cggggccaag 2400

gggctgcagg cgcggctggt ggtgctgccc gataccatcg ccagcccccg ctctgacacc 2460

aacatcctgt ggaaaaagga ccagcagacc ggtctcgaca tccccatctg ggtgccccgc 2520

cgcgagctga ccaccgacct gaccgcagcc ttgcaggaca agatcaaaca ggaggcggcg 2580

gaggaatata accgtctgct ttacgtggcg ttgacccgtg cgagcgaccg gctggtcata 2640

tgcggctgga agcccagccg gggcgtgccc gatggctgct ggtatgacct gtgccaccgt 2700

ggtttcgagc aggcaggcgc cgaggcccgg ccttttgatc tgggctggga gggtgaaagc 2760

ctggtgctgg aggaaaagcg cagcgtgtcc gcgcccatgc cccggtcgca ggccccggcg 2820

ctggaggtgg aacccgtcgc cttgccggag tggatgggcc acgcgccgct gtggcggccc 2880

gtgctgcccg tggcggaagg cccgctggcc cgcccgcttg cgcccagccg gcccgatgat 2940

gtggcccttg gcccgcagcc tgccgtgcgc tcgccgctgg tctcggccag cacggtgcgc 3000

gaccgggcgg atgccacggc ccggcgcgcc cgcgccctgc aacgcgggca actggtgcat 3060

gccctgttgc agtacctgcc cgactgcgcg cccgatgcgc gtgcggatct ggcccatgcc 3120

tggctgtcgc gcccggcggc ggggcttgcg cccgaactgc gtgatgaact ggtggcggag 3180

gtgctcgcgg tcatggagcg gccagagctt gcggccctgt ttgcgcctgg cagccgcgtg 3240

gagcagcccc tggccggaat cgtgggcgag caggtgatcg tggggcaggt ggaccgcatg 3300

gcggtcaatg ccgatacggt catggtgtgc gacttcaaga ccaaccgcca tccaccagcc 3360

gatattgggc agacccccgt gctctacctg cgccagatgg cggcctaccg cgccctgttg 3420

cgcggggtct atcccggcag gcaggttgtg tgcgtgctgg tctggaccga gggcgtgcgc 3480

gttgacatcc tgcccgcggc actgctggac cattatgcgc cggaccgggt tgccttaaag 3540

gggtcggtac gggcttga 3558

<210> 22

<211> 1185

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2950 (BcsA I)

<400> 22

Met Asp Ala Ile Gly Leu Ala Asn Arg Ser Gln Ala Gln Ala Ser Asp

1 5 10 15

Pro Gln Ala Ser Val Phe Val Ser Ala Ser Ala Gly Ser Gly Lys Thr

20 25 30

Lys Leu Leu Ile Asp Arg Leu Leu Arg Leu Met Leu Pro Arg Asp Val

35 40 45

Pro Asp Arg Asp Gly Val Pro Thr Arg Val Ala Gly Ser Asp Pro Gly

50 55 60

Arg Ile Leu Cys Leu Thr Phe Thr Lys Ala Ala Ala Ala Glu Met Ser

65 70 75 80

Ile Arg Leu Gln Asp Arg Leu Gly Gln Trp Val Met Leu Pro Asp Ala

85 90 95

Ala Leu Asp Gly Glu Leu Ala Arg Leu Ser Val Pro Val Asp Glu Arg

100 105 110

Thr Arg Glu Val Ala Arg Glu Leu Phe Ala Arg Val Leu Asp Leu Pro

115 120 125

Gly Gly Met Arg Ile Gly Thr Ile His Ala Phe Cys Gln Ser Leu Leu

130 135 140

Arg Arg Phe Pro Ile Glu Ala Ala Ile Ser Pro His Phe Thr Leu Ile

145 150 155 160

Glu Glu Thr Asp Ala Arg Leu Ala Gln Arg Ala Ala Val Glu Asp Val

165 170 175

Val Gly Ala Gly Gly Pro Ala Val Gly Leu Leu Ala Gly Gln Ile Gly

180 185 190

Met Gly Asp Phe Thr Ser Leu Ile Ala Arg Leu Gln Ala His Ala Arg

195 200 205

Arg Leu Leu Pro Val Ala Arg Gln Trp Val Arg Asp Arg Ala Ser Val

210 215 220

Glu Ala Ala Leu Met Arg Leu Leu Gly Ile Ser Gly Arg Ser Val Glu

225 230 235 240

Glu Val Leu Arg Gln Ala Cys Ala Thr Ile Pro Asp Glu Asp Ala Leu

245 250 255

Arg Asp Gly Leu Arg Tyr Ala Ala Gln Glu Gly Ser Pro Thr Val Arg

260 265 270

Thr Gln Ala Glu Ile Met Leu Ala Trp Leu Gly Gln Asp Ala Ala Ala

275 280 285

Arg Ala Ala Gln Trp Pro Val Trp Arg Lys Ala Leu Leu Lys Asn Asp

290 295 300

Gly Ala Pro Arg Gln Arg Gly Gly Leu Asn Ala Lys Leu Ala Ala Ala

305 310 315 320

Arg Pro Val Leu Val Glu Gln Leu Leu Ala Glu Ala Ala Arg Ile Ile

325 330 335

Ala Ile Glu Gln Gln Met Arg Ala Ile Thr Val Phe Glu Leu Thr Cys

340 345 350

Ala Leu Leu Ser Val Ala Gln Pro Val Leu Glu Arg Tyr Ala Thr Arg

355 360 365

Lys Ser Ala Gln Gly Met Val Asp Tyr Asp Asp Leu Ile Asp Arg Thr

370 375 380

Leu Glu Leu Leu Arg Asp Pro Gly Ala Ala Trp Val Leu Tyr Lys Leu

385 390 395 400

Asp Gly Gly Ile Asp His Leu Leu Leu Asp Glu Val Gln Asp Thr Ser

405 410 415

Phe Asp Gln Trp Ala Ile Ala Gly Gly Leu Thr Ala Glu Phe Phe Ala

420 425 430

Gly Glu Gly Thr His Asp Asp Glu Gly Thr Pro Arg Thr Ile Phe Ala

435 440 445

Val Gly Asp Tyr Lys Gln Ser Ile Tyr Ser Phe Gln Gly Ala Asp Pro

450 455 460

Glu Ala Phe Arg Ala Trp Arg Gln Arg Phe Arg His Ser Ala Ala Ala

465 470 475 480

Ala Gly Ala Leu Trp Arg Glu Pro Ala Leu Thr Val Ser Phe Arg Ser

485 490 495

Thr Ala Pro Val Leu Lys Leu Val Asp Ser Val Phe Ala Asn Ala Ala

500 505 510

Ala Ala Arg Gly Val Ala Glu Pro Asp Gly Thr Ile Pro Pro His Val

515 520 525

Thr Ala Arg Pro Gly Gln Gly Gly Arg Val Glu Ile Trp Pro Pro Val

530 535 540

Pro Val Glu Glu Asp Gly Glu Ala Val Ser Pro Trp Gln Ala Pro Ser

545 550 555 560

Gln Asn Ala Gly Gln Ser Thr Ala Gln Gln Arg Leu Ala Asp Thr Leu

565 570 575

Ala Ala Trp Ile Ala Ala Glu Leu Arg Arg Pro Pro Ala Pro Gly Glu

580 585 590

Ala Pro Leu Ala Pro Gly Asp Val Leu Ile Leu Val Pro Arg Arg Ser

595 600 605

Ala Phe Ala Arg Ala Leu Ile Arg Ala Leu Lys Thr Asn Asp Val Pro

610 615 620

Val Ala Thr Leu Val Arg Thr Val Leu Thr Asp Gln Leu Ala Val Gln

625 630 635 640

Asp Leu Met Ala Leu Cys Ala Cys Leu Leu Leu Pro Gln Asp Asp Leu

645 650 655

Thr Leu Ala Cys Val Leu Thr Ser Pro Ile Gly Gly Leu Asp Asp Glu

660 665 670

Ser Leu Met His Leu Ala Thr Gly Arg Asp Gly Lys Pro Leu Trp Ala

675 680 685

Val Leu Arg Ala Arg His Thr Glu Arg Pro Asp Trp Ala Ala Ala Trp

690 695 700

His Ile Leu Asn Thr Leu Phe Arg Gln Val Asp Tyr Ala Thr Pro Tyr

705 710 715 720

Gln Leu Leu Ala Glu Ala Leu Gly Pro Leu Gly Ala Arg Thr Arg Leu

725 730 735

Leu Ala Arg Leu Gly Pro Glu Ala Val Glu Pro Val Asp Glu Leu Leu

740 745 750

Ser Ala Ala Leu Arg Phe Glu Glu Ala His Ala Ile Ser Leu Gln Gly

755 760 765

Phe Leu His Trp Leu Asn Ala Ser Asp Glu Thr Val Arg His Glu Pro

770 775 780

Asp Ala Ser Ala Asn Met Val Arg Val Met Thr Ala His Gly Ala Lys

785 790 795 800

Gly Leu Gln Ala Arg Leu Val Val Leu Pro Asp Thr Ile Ala Ser Pro

805 810 815

Arg Ser Asp Thr Asn Ile Leu Trp Lys Lys Asp Gln Gln Thr Gly Leu

820 825 830

Asp Ile Pro Ile Trp Val Pro Arg Arg Glu Leu Thr Thr Asp Leu Thr

835 840 845

Ala Ala Leu Gln Asp Lys Ile Lys Gln Glu Ala Ala Glu Glu Tyr Asn

850 855 860

Arg Leu Leu Tyr Val Ala Leu Thr Arg Ala Ser Asp Arg Leu Val Ile

865 870 875 880

Cys Gly Trp Lys Pro Ser Arg Gly Val Pro Asp Gly Cys Trp Tyr Asp

885 890 895

Leu Cys His Arg Gly Phe Glu Gln Ala Gly Ala Glu Ala Arg Pro Phe

900 905 910

Asp Leu Gly Trp Glu Gly Glu Ser Leu Val Leu Glu Glu Lys Arg Ser

915 920 925

Val Ser Ala Pro Met Pro Arg Ser Gln Ala Pro Ala Leu Glu Val Glu

930 935 940

Pro Val Ala Leu Pro Glu Trp Met Gly His Ala Pro Leu Trp Arg Pro

945 950 955 960

Val Leu Pro Val Ala Glu Gly Pro Leu Ala Arg Pro Leu Ala Pro Ser

965 970 975

Arg Pro Asp Asp Val Ala Leu Gly Pro Gln Pro Ala Val Arg Ser Pro

980 985 990

Leu Val Ser Ala Ser Thr Val Arg Asp Arg Ala Asp Ala Thr Ala Arg

995 1000 1005

Arg Ala Arg Ala Leu Gln Arg Gly Gln Leu Val His Ala Leu Leu Gln

1010 1015 1020

Tyr Leu Pro Asp Cys Ala Pro Asp Ala Arg Ala Asp Leu Ala His Ala

1025 1030 1035 1040

Trp Leu Ser Arg Pro Ala Ala Gly Leu Ala Pro Glu Leu Arg Asp Glu

1045 1050 1055

Leu Val Ala Glu Val Leu Ala Val Met Glu Arg Pro Glu Leu Ala Ala

1060 1065 1070

Leu Phe Ala Pro Gly Ser Arg Val Glu Gln Pro Leu Ala Gly Ile Val

1075 1080 1085

Gly Glu Gln Val Ile Val Gly Gln Val Asp Arg Met Ala Val Asn Ala

1090 1095 1100

Asp Thr Val Met Val Cys Asp Phe Lys Thr Asn Arg His Pro Pro Ala

1105 1110 1115 1120

Asp Ile Gly Gln Thr Pro Val Leu Tyr Leu Arg Gln Met Ala Ala Tyr

1125 1130 1135

Arg Ala Leu Leu Arg Gly Val Tyr Pro Gly Arg Gln Val Val Cys Val

1140 1145 1150

Leu Val Trp Thr Glu Gly Val Arg Val Asp Ile Leu Pro Ala Ala Leu

1155 1160 1165

Leu Asp His Tyr Ala Pro Asp Arg Val Ala Leu Lys Gly Ser Val Arg

1170 1175 1180

Ala

118

<210> 23

<211> 258

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2951 (BcsB I)

<400> 23

gtggcggaac tggtctactg gcggctgacc ggcggccatg tgccagccct ggtgctcgac 60

gtggcgcgcg gggaggagct gaccgatctg atcgcgtcat gccgcgcggg gctgcgtgat 120

ctggtggccg gttacgacaa tcccgaccag ccctatctgt cgcaccccta tcccggcgag 180

gaaccccgct ttgccgatta cgcccatctg gcccgcgttg cggaatggag tgcggcgcgt 240

gaggagaatg gcggatga 258

<210> 24

<211> 85

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2951 (BcsB I)

<400> 24

Met Ala Glu Leu Val Tyr Trp Arg Leu Thr Gly Gly His Val Pro Ala

1 5 10 15

Leu Val Leu Asp Val Ala Arg Gly Glu Glu Leu Thr Asp Leu Ile Ala

20 25 30

Ser Cys Arg Ala Gly Leu Arg Asp Leu Val Ala Gly Tyr Asp Asn Pro

35 40 45

Asp Gln Pro Tyr Leu Ser His Pro Tyr Pro Gly Glu Glu Pro Arg Phe

50 55 60

Ala Asp Tyr Ala His Leu Ala Arg Val Ala Glu Trp Ser Ala Ala Arg

65 70 75 80

Glu Glu Asn Gly Gly

85

<210> 25

<211> 2823

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2952 (BcsC I)

<400> 25

gtgagccagc ccgaagcccc tgccctgcgc ggtcgggcgg ccgtcattcc gccgcatgtg 60

ccatttgtgg accagattgc cgcgcgctgg ctggcgcagg cggggcatga cgcgcaggcc 120

tgcggcaccg gcctgatcct gctgcccagc cgccgcgccg cccgcgccct gaccgaggcc 180

ttcgtgcggc aggtcgatgg tcgccccatc ctgctgccgc gcatcgcccc cattgccggg 240

cttgatgagg cggcccttgc gctctcgggt cgcaatgcgc ttgacctgcc gcccgcggtc 300

gaccccatcc gccgccttgc cacgcttacg ctgctggtca tgcaggcagg ccgcgccttt 360

ggtgatgtgc agggcgtaga ccaggcctgg ccgctggccc gcgcgctggc tgacctgatg 420

gatgaggcgg aatgggccga atgcgacctg tgcgagcgcc tgccccatgc cgccgagggc 480

gattttgccc agcactggca cctgaccgtg cagttcctgt ccatcatcac ccaggtctgg 540

cccgcatggc tggccgagca gggggtgatg aaccccgtcg cccgccagac tgccctgctg 600

catgcgcagg ccgcgcgctg gctggaggtg ccaccaccgg caggctaccc catctgggcg 660

gcaggttttt ccgatgccgt gccctccacc atcgccatgc tgcgcgccgt gctgtcgctg 720

cccgatgggc tgctggtgct gccgggcgtg gatatggact gcgccgataa cgtctggtgc 780

aacttgccgc ccgaccaccc gcaggcgggc accgcgcaca tgctggccga actcggcctt 840

gagcgggcct gcatggaaaa gtgggaggat gtgccaccgg gatgcgttgc cagcagcccc 900

atcccgcgcg ccagcctgct ggcccgcgcc ctgctgcccg cccatgcgct ggcgggctgg 960

cgcgaccccg aggctccggt catggccgat ggcctgtcgg tactgcgcag caccgaccag 1020

caggaggaag cggcggccat tgcgatggtg ctgcgccagg tgctcgagca gcccggcagg 1080

cgcgccgccc tcgtcacccc cgaccgtgcg ctggccgggc gcgttgcgac cgaactggtg 1140

cgctggggcg tcattgccga tgacagcgcg ggcgaaagcc tgctcaccat accgcagacc 1200

gctttcctgc ggctgatcat acaggcggtg gatggcgggc tttcgcccgt ggcgctgctt 1260

tcggtcatca agcacccgct ggcggcgtgt ggccttgcgc cgggcaactg ccgcgccagc 1320

gcccgccagc tcgagcggct ggtgctgcgc ggccccgccc cgccaccggg cattgccggg 1380

ctgaggggcg cgctggcgcg ggcggcgcaa gacccgcacg gcgcgctggc cgatgcgccc 1440

gacgcgccgg aggaaatcac cggcttcatc aaccggctcg aaacagcttt cggtccgctg 1500

ctcgccctgc cgcgcgcgag tctggtgccg gtttccaccc tgcttgccgc cctgatcgag 1560

accgcgcagg cgcttgccgc gaccgacacc accgatggcg ccgagcgcct gtgggccggt 1620

gaggaaggca acgcgctggg tcagcacatg agtggcatgc tggcatggtg cgacgtgctg 1680

cccgaggccc ggctgggcgc gctggacggg ctgctggcct cctcgctggc gggcatgacg 1740

gtgcaggggc ggcgcgcggt gcgtgggcgc gagggcacgg cggtgcatcc gcgcgtgttc 1800

atctggggcc tgcttgaagc gcgattgcag acggccgaca ccatcgtgct tggcggcctg 1860

gtcgagaccg tctggccccc cgccaccgat ccagggccat ggatgagccg ccccatgcgc 1920

acccgcgtgg gtctgccctc gccggaatgg gccataggcc aggccgcgca tgacttcgtc 1980

tcgtgcgcct gtgctgcgcc aagggtcgtg ctgtccatgg cggcacggcg gcagggcgcg 2040

ccgaccgtgc ccgcgcggtg gctggtcagg ctcaatgcct atctggcggg gcacggctac 2100

ggcctgccgc cgcaccccgc cctgcgctgg cttgacagcc tcgaccgccc gcccggcggg 2160

ctggtgcagc ctgccagccc gccgcgtccg tgcccggcag tcgccctgcg ccctcgcagc 2220

ctgtcagtga ccgagatcga gacatggatg cgcgacccct acgccatcta tgcccgccgc 2280

atcctgcgcc tcaaccgcct gcccgacctt gaggaactgg cggatgctgc ggattacggg 2340

cagatcgtgc acgcggccct tgatgagtgg ttcagcgccc accccaccga ctggccggca 2400

gagggcgcga ccgaaatgca ggccgtcttt accgatgtgc tgcgcagggc tgaactgcgc 2460

ccggcccttg ccgcctggtg ggcgccaagg ctggaacgca tcgccacatg gtgcgcccgg 2520

accgaacagg cgcggcgggc cacgggtggc gcgcgtgagg tgctgaccga actgagcgga 2580

cgcatgcaac tcgaaggcct gcccggcggc cccttcaccc tgcgcggccg ggccgaccgg 2640

attgatcgca acggtgatgg cacgctgagc ctgttcgatt acaagaccgg caccctgccc 2700

acgcggcgca gcgtgctgga gggctggcag tcgcaactgg tgcttgaggc cgcgatgatt 2760

gaaaatggcg gctttccgcc cctatcgcgg gcacggtggc ggaactggtc tactggcggc 2820

tga 2823

<210> 26

<211> 940

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2952 (BcsC I)

<400> 26

Met Ser Gln Pro Glu Ala Pro Ala Leu Arg Gly Arg Ala Ala Val Ile

1 5 10 15

Pro Pro His Val Pro Phe Val Asp Gln Ile Ala Ala Arg Trp Leu Ala

20 25 30

Gln Ala Gly His Asp Ala Gln Ala Cys Gly Thr Gly Leu Ile Leu Leu

35 40 45

Pro Ser Arg Arg Ala Ala Arg Ala Leu Thr Glu Ala Phe Val Arg Gln

50 55 60

Val Asp Gly Arg Pro Ile Leu Leu Pro Arg Ile Ala Pro Ile Ala Gly

65 70 75 80

Leu Asp Glu Ala Ala Leu Ala Leu Ser Gly Arg Asn Ala Leu Asp Leu

85 90 95

Pro Pro Ala Val Asp Pro Ile Arg Arg Leu Ala Thr Leu Thr Leu Leu

100 105 110

Val Met Gln Ala Gly Arg Ala Phe Gly Asp Val Gln Gly Val Asp Gln

115 120 125

Ala Trp Pro Leu Ala Arg Ala Leu Ala Asp Leu Met Asp Glu Ala Glu

130 135 140

Trp Ala Glu Cys Asp Leu Cys Glu Arg Leu Pro His Ala Ala Glu Gly

145 150 155 160

Asp Phe Ala Gln His Trp His Leu Thr Val Gln Phe Leu Ser Ile Ile

165 170 175

Thr Gln Val Trp Pro Ala Trp Leu Ala Glu Gln Gly Val Met Asn Pro

180 185 190

Val Ala Arg Gln Thr Ala Leu Leu His Ala Gln Ala Ala Arg Trp Leu

195 200 205

Glu Val Pro Pro Pro Ala Gly Tyr Pro Ile Trp Ala Ala Gly Phe Ser

210 215 220

Asp Ala Val Pro Ser Thr Ile Ala Met Leu Arg Ala Val Leu Ser Leu

225 230 235 240

Pro Asp Gly Leu Leu Val Leu Pro Gly Val Asp Met Asp Cys Ala Asp

245 250 255

Asn Val Trp Cys Asn Leu Pro Pro Asp His Pro Gln Ala Gly Thr Ala

260 265 270

His Met Leu Ala Glu Leu Gly Leu Glu Arg Ala Cys Met Glu Lys Trp

275 280 285

Glu Asp Val Pro Pro Gly Cys Val Ala Ser Ser Pro Ile Pro Arg Ala

290 295 300

Ser Leu Leu Ala Arg Ala Leu Leu Pro Ala His Ala Leu Ala Gly Trp

305 310 315 320

Arg Asp Pro Glu Ala Pro Val Met Ala Asp Gly Leu Ser Val Leu Arg

325 330 335

Ser Thr Asp Gln Gln Glu Glu Ala Ala Ala Ile Ala Met Val Leu Arg

340 345 350

Gln Val Leu Glu Gln Pro Gly Arg Arg Ala Ala Leu Val Thr Pro Asp

355 360 365

Arg Ala Leu Ala Gly Arg Val Ala Thr Glu Leu Val Arg Trp Gly Val

370 375 380

Ile Ala Asp Asp Ser Ala Gly Glu Ser Leu Leu Thr Ile Pro Gln Thr

385 390 395 400

Ala Phe Leu Arg Leu Ile Ile Gln Ala Val Asp Gly Gly Leu Ser Pro

405 410 415

Val Ala Leu Leu Ser Val Ile Lys His Pro Leu Ala Ala Cys Gly Leu

420 425 430

Ala Pro Gly Asn Cys Arg Ala Ser Ala Arg Gln Leu Glu Arg Leu Val

435 440 445

Leu Arg Gly Pro Ala Pro Pro Pro Gly Ile Ala Gly Leu Arg Gly Ala

450 455 460

Leu Ala Arg Ala Ala Gln Asp Pro His Gly Ala Leu Ala Asp Ala Pro

465 470 475 480

Asp Ala Pro Glu Glu Ile Thr Gly Phe Ile Asn Arg Leu Glu Thr Ala

485 490 495

Phe Gly Pro Leu Leu Ala Leu Pro Arg Ala Ser Leu Val Pro Val Ser

500 505 510

Thr Leu Leu Ala Ala Leu Ile Glu Thr Ala Gln Ala Leu Ala Ala Thr

515 520 525

Asp Thr Thr Asp Gly Ala Glu Arg Leu Trp Ala Gly Glu Glu Gly Asn

530 535 540

Ala Leu Gly Gln His Met Ser Gly Met Leu Ala Trp Cys Asp Val Leu

545 550 555 560

Pro Glu Ala Arg Leu Gly Ala Leu Asp Gly Leu Leu Ala Ser Ser Leu

565 570 575

Ala Gly Met Thr Val Gln Gly Arg Arg Ala Val Arg Gly Arg Glu Gly

580 585 590

Thr Ala Val His Pro Arg Val Phe Ile Trp Gly Leu Leu Glu Ala Arg

595 600 605

Leu Gln Thr Ala Asp Thr Ile Val Leu Gly Gly Leu Val Glu Thr Val

610 615 620

Trp Pro Pro Ala Thr Asp Pro Gly Pro Trp Met Ser Arg Pro Met Arg

625 630 635 640

Thr Arg Val Gly Leu Pro Ser Pro Glu Trp Ala Ile Gly Gln Ala Ala

645 650 655

His Asp Phe Val Ser Cys Ala Cys Ala Ala Pro Arg Val Val Leu Ser

660 665 670

Met Ala Ala Arg Arg Gln Gly Ala Pro Thr Val Pro Ala Arg Trp Leu

675 680 685

Val Arg Leu Asn Ala Tyr Leu Ala Gly His Gly Tyr Gly Leu Pro Pro

690 695 700

His Pro Ala Leu Arg Trp Leu Asp Ser Leu Asp Arg Pro Pro Gly Gly

705 710 715 720

Leu Val Gln Pro Ala Ser Pro Pro Arg Pro Cys Pro Ala Val Ala Leu

725 730 735

Arg Pro Arg Ser Leu Ser Val Thr Glu Ile Glu Thr Trp Met Arg Asp

740 745 750

Pro Tyr Ala Ile Tyr Ala Arg Arg Ile Leu Arg Leu Asn Arg Leu Pro

755 760 765

Asp Leu Glu Glu Leu Ala Asp Ala Ala Asp Tyr Gly Gln Ile Val His

770 775 780

Ala Ala Leu Asp Glu Trp Phe Ser Ala His Pro Thr Asp Trp Pro Ala

785 790 795 800

Glu Gly Ala Thr Glu Met Gln Ala Val Phe Thr Asp Val Leu Arg Arg

805 810 815

Ala Glu Leu Arg Pro Ala Leu Ala Ala Trp Trp Ala Pro Arg Leu Glu

820 825 830

Arg Ile Ala Thr Trp Cys Ala Arg Thr Glu Gln Ala Arg Arg Ala Thr

835 840 845

Gly Gly Ala Arg Glu Val Leu Thr Glu Leu Ser Gly Arg Met Gln Leu

850 855 860

Glu Gly Leu Pro Gly Gly Pro Phe Thr Leu Arg Gly Arg Ala Asp Arg

865 870 875 880

Ile Asp Arg Asn Gly Asp Gly Thr Leu Ser Leu Phe Asp Tyr Lys Thr

885 890 895

Gly Thr Leu Pro Thr Arg Arg Ser Val Leu Glu Gly Trp Gln Ser Gln

900 905 910

Leu Val Leu Glu Ala Ala Met Ile Glu Asn Gly Gly Phe Pro Pro Leu

915 920 925

Ser Arg Ala Arg Trp Arg Asn Trp Ser Thr Gly Gly

930 935 940

<210> 27

<211> 762

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2953 (BcsD I)

<400> 27

atgagcgtga acatgccccg taccgccatg gtttttgccg cagggctggg gcggcgcatg 60

cggccgctga gcgaagcggt gcccaagccg ttgctgcgcg tggcgggtca gccgattctc 120

gatcatgtgc ttgaccggct cgaggcggcg ggtgttgcgc aggtggtggt caatgcccac 180

tggcagcccg atgccatcca tgcggcgctg gccgcgcggg cggctgccca tcgtggcccc 240

cgcacggttg aacaggtgga ggaaaccctg cttgaaaccg gcggcagtgc cgcaagtgcc 300

ctgcgcgcgg gccggatcgg gccggacccg ttctttctgc tcaatggcga tgccatgtgg 360

ctcaacggcc cggtgcctgc cctgcggcgg ctggcggcgg cgttcgaccc tgccagcatg 420

gacgccatgc tgctgcttgg cggcatgacg cgtgcggtgg gcgaggtggg ccatggcgat 480

tttgccgtcg atgcccacgg caggccgcgc cgcccgcgcg cgggcgagat tacgccctat 540

atctttaccg gcgtgcagat ctgctcgccc cggctgttcg atgcagcgcc cgaaggcggc 600

ttcagcatga accggctgtg ggataaggca atggaggcag gccgcctggg tgtgatcgtg 660

catgattcgc tgtggttcca cctctcacgc ccggccgata tcgcggccgc cgagcgcgtg 720

ctgcattcaa ccctgaaccc cgattcggat tccggcctgt ga 762

<210> 28

<211> 253

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_2953 (BcsD I)

<400> 28

Met Ser Val Asn Met Pro Arg Thr Ala Met Val Phe Ala Ala Gly Leu

1 5 10 15

Gly Arg Arg Met Arg Pro Leu Ser Glu Ala Val Pro Lys Pro Leu Leu

20 25 30

Arg Val Ala Gly Gln Pro Ile Leu Asp His Val Leu Asp Arg Leu Glu

35 40 45

Ala Ala Gly Val Ala Gln Val Val Val Asn Ala His Trp Gln Pro Asp

50 55 60

Ala Ile His Ala Ala Leu Ala Ala Arg Ala Ala Ala His Arg Gly Pro

65 70 75 80

Arg Thr Val Glu Gln Val Glu Glu Thr Leu Leu Glu Thr Gly Gly Ser

85 90 95

Ala Ala Ser Ala Leu Arg Ala Gly Arg Ile Gly Pro Asp Pro Phe Phe

100 105 110

Leu Leu Asn Gly Asp Ala Met Trp Leu Asn Gly Pro Val Pro Ala Leu

115 120 125

Arg Arg Leu Ala Ala Ala Phe Asp Pro Ala Ser Met Asp Ala Met Leu

130 135 140

Leu Leu Gly Gly Met Thr Arg Ala Val Gly Glu Val Gly His Gly Asp

145 150 155 160

Phe Ala Val Asp Ala His Gly Arg Pro Arg Arg Pro Arg Ala Gly Glu

165 170 175

Ile Thr Pro Tyr Ile Phe Thr Gly Val Gln Ile Cys Ser Pro Arg Leu

180 185 190

Phe Asp Ala Ala Pro Glu Gly Gly Phe Ser Met Asn Arg Leu Trp Asp

195 200 205

Lys Ala Met Glu Ala Gly Arg Leu Gly Val Ile Val His Asp Ser Leu

210 215 220

Trp Phe His Leu Ser Arg Pro Ala Asp Ile Ala Ala Ala Glu Arg Val

225 230 235 240

Leu His Ser Thr Leu Asn Pro Asp Ser Asp Ser Gly Leu

245 250

<210> 29

<211> 387

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_717 (BcsAB II)

<400> 29

atgatgcgtg atgacggggc gctggccatc ttgttgtatg atggcgcgta tgcccgcgcg 60

cattatgcct ttgtggtggc tgcgggcgcg ctggccattg gccgcccggt catcatgttc 120

gcggcggggc ggggcgtgca cgcgctggcg cgcgactggc gtggcctgca tgatgcggag 180

gatgatgcag gcgtgcgtgc gcgtggcgtt gcgggcatcg acacgctgcg tgaagccacg 240

attgaactgg aggcgacgct catggtgtgc gaggccgcgc tgaaactgtg cgatcttgcg 300

cccgggcagt tgcttgatgg cgtacaggtg gccggtattc ccacttttct cgaagccgcc 360

cgggggcggc agatcataac cctttaa 387

<210> 30

<211> 128

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_717 (BcsAB II)

<400> 30

Met Met Arg Asp Asp Gly Ala Leu Ala Ile Leu Leu Tyr Asp Gly Ala

1 5 10 15

Tyr Ala Arg Ala His Tyr Ala Phe Val Val Ala Ala Gly Ala Leu Ala

20 25 30

Ile Gly Arg Pro Val Ile Met Phe Ala Ala Gly Arg Gly Val His Ala

35 40 45

Leu Ala Arg Asp Trp Arg Gly Leu His Asp Ala Glu Asp Asp Ala Gly

50 55 60

Val Arg Ala Arg Gly Val Ala Gly Ile Asp Thr Leu Arg Glu Ala Thr

65 70 75 80

Ile Glu Leu Glu Ala Thr Leu Met Val Cys Glu Ala Ala Leu Lys Leu

85 90 95

Cys Asp Leu Ala Pro Gly Gln Leu Leu Asp Gly Val Gln Val Ala Gly

100 105 110

Ile Pro Thr Phe Leu Glu Ala Ala Arg Gly Arg Gln Ile Ile Thr Leu

115 120 125

<210> 31

<211> 1104

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_1148 (BcsAB II)

<400> 31

atgcgcgtcc ctgaggaatc gatcggtacg ccttctgttt cctcctccgc gggcctgtca 60

gcttatgcgc gtgaggtgcg ggggcgctac catgggctgc tgcggcggcg catggggctg 120

cttggcctgc tgctcgcgct gatcgtggcc tcggtggtgc ttgatttctc gctcgggcca 180

tcggggcttt cgccttcagc cctcatccgc accctgctgc acccgcatga cgtgcccgcc 240

gggcagggcg tgatcgtgtg gcagatcagg ctaccctacg cgcttatggc cgtatgtgtc 300

ggggcgtcgc tggggctggc tggggcggaa atgcagaccg tgctcgccaa cccgctagca 360

agtcccttca cgctcggggt ctcggcggct gcggcgtttg gcgcgtcact cgccatcatt 420

cttggctggc ggctgcccgg cgtgcccgat gcgtgggtgg tgtcggggga tgcctttgtc 480

tttgccatag gctccgtgct gctgctcgac atggtctcgc gcaggcgcaa tgcgggcacc 540

ggcacggtgg tgctgtttgg cattgcgctg gtgttcgcct tccatgcgct ggtgtcgctg 600

ctgcagtttg tggccaatga agatgcgttg caggacctgg tgttctggac catgggcagc 660

ctgacacgcg caacatggcc caagctgggc ctgctggccg ccgcctgcgt gatcgtggcc 720

ccgctttcct ttgctgcgtc atggaagctg accgtgctgc gcatgggcga ggaacgtgcc 780

gcgagcctgg gcgtggacgt gccgcgcgtg cggcgtggcg cgttgctgcg ggtgagcatc 840

ctgtccgctc tggccgtgtc cttcgtgggt acgatcggtt tcgtggggtt ggtagcgccg 900

catatcgccc gccgcatgct gggcgaggac catcgtttct acctgccggg cagcatgctg 960

gtgggcgcgc tggtgatgtc gctttcatcc atcgcggcgc gcaacctgct gccaggcgtg 1020

gtcattccca ccgggatcgt gaccgcgctg gtgggcattc cgttctttct ggccattgtg 1080

ctgcgcaggc ggagcatgac ctga 1104

<210> 32

<211> 367

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_1148 (BcsAB II)

<400> 32

Met Arg Val Pro Glu Glu Ser Ile Gly Thr Pro Ser Val Ser Ser Ser

1 5 10 15

Ala Gly Leu Ser Ala Tyr Ala Arg Glu Val Arg Gly Arg Tyr His Gly

20 25 30

Leu Leu Arg Arg Arg Met Gly Leu Leu Gly Leu Leu Leu Ala Leu Ile

35 40 45

Val Ala Ser Val Val Leu Asp Phe Ser Leu Gly Pro Ser Gly Leu Ser

50 55 60

Pro Ser Ala Leu Ile Arg Thr Leu Leu His Pro His Asp Val Pro Ala

65 70 75 80

Gly Gln Gly Val Ile Val Trp Gln Ile Arg Leu Pro Tyr Ala Leu Met

85 90 95

Ala Val Cys Val Gly Ala Ser Leu Gly Leu Ala Gly Ala Glu Met Gln

100 105 110

Thr Val Leu Ala Asn Pro Leu Ala Ser Pro Phe Thr Leu Gly Val Ser

115 120 125

Ala Ala Ala Ala Phe Gly Ala Ser Leu Ala Ile Ile Leu Gly Trp Arg

130 135 140

Leu Pro Gly Val Pro Asp Ala Trp Val Val Ser Gly Asp Ala Phe Val

145 150 155 160

Phe Ala Ile Gly Ser Val Leu Leu Leu Asp Met Val Ser Arg Arg Arg

165 170 175

Asn Ala Gly Thr Gly Thr Val Val Leu Phe Gly Ile Ala Leu Val Phe

180 185 190

Ala Phe His Ala Leu Val Ser Leu Leu Gln Phe Val Ala Asn Glu Asp

195 200 205

Ala Leu Gln Asp Leu Val Phe Trp Thr Met Gly Ser Leu Thr Arg Ala

210 215 220

Thr Trp Pro Lys Leu Gly Leu Leu Ala Ala Ala Cys Val Ile Val Ala

225 230 235 240

Pro Leu Ser Phe Ala Ala Ser Trp Lys Leu Thr Val Leu Arg Met Gly

245 250 255

Glu Glu Arg Ala Ala Ser Leu Gly Val Asp Val Pro Arg Val Arg Arg

260 265 270

Gly Ala Leu Leu Arg Val Ser Ile Leu Ser Ala Leu Ala Val Ser Phe

275 280 285

Val Gly Thr Ile Gly Phe Val Gly Leu Val Ala Pro His Ile Ala Arg

290 295 300

Arg Met Leu Gly Glu Asp His Arg Phe Tyr Leu Pro Gly Ser Met Leu

305 310 315 320

Val Gly Ala Leu Val Met Ser Leu Ser Ser Ile Ala Ala Arg Asn Leu

325 330 335

Leu Pro Gly Val Val Ile Pro Thr Gly Ile Val Thr Ala Leu Val Gly

340 345 350

Ile Pro Phe Phe Leu Ala Ile Val Leu Arg Arg Arg Ser Met Thr

355 360 365

<210> 33

<211> 438

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_718 (BcsX)

<400> 33

atggcgcagt gcgtgcggca tgaattcctt gcgcgggcgg ccagcaggcc ctcgcgtgtc 60

acccggcggt gggggtatct ggcggcctgc gctgcggtgc tggccatggg ggcagggcca 120

gtacgggccg cgacggtggc ggcccaccat catgcccatg cggccgcgac caaggcagcg 180

accagtcttg gccacgccca tgtgcatggg gccgccagga ccgcaaaaac ggcggaaaaa 240

aaggctgaaa agaccacgca tgaggcaggc cgtcaccacc atgcggcagc ggttgccgcc 300

ggtgctggcg caggcgccgt ggcgggtgtg ggggttgctg cggctggcac ggcccatccg 360

gcccccgcca cgccaccacc tgccccccgg ccgatgccgc ggcactcgcg cccgttgata 420

acaccaaggg cagcgtga 438

<210> 34

<211> 145

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_718 (BcsX)

<400> 34

Met Ala Gln Cys Val Arg His Glu Phe Leu Ala Arg Ala Ala Ser Arg

1 5 10 15

Pro Ser Arg Val Thr Arg Arg Trp Gly Tyr Leu Ala Ala Cys Ala Ala

20 25 30

Val Leu Ala Met Gly Ala Gly Pro Val Arg Ala Ala Thr Val Ala Ala

35 40 45

His His His Ala His Ala Ala Ala Thr Lys Ala Ala Thr Ser Leu Gly

50 55 60

His Ala His Val His Gly Ala Ala Arg Thr Ala Lys Thr Ala Glu Lys

65 70 75 80

Lys Ala Glu Lys Thr Thr His Glu Ala Gly Arg His His His Ala Ala

85 90 95

Ala Val Ala Ala Gly Ala Gly Ala Gly Ala Val Ala Gly Val Gly Val

100 105 110

Ala Ala Ala Gly Thr Ala His Pro Ala Pro Ala Thr Pro Pro Pro Ala

115 120 125

Pro Arg Pro Met Pro Arg His Ser Arg Pro Leu Ile Thr Pro Arg Ala

130 135 140

Ala

145

<210> 35

<211> 291

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_719 (BcsY)

<400> 35

gtgaccggcc tgcccctgcc gcgctttgcc gccttccgcg ccgatgaggt caacctgcgc 60

accgggccgg ggcagcgtta ccccattgac tgggtgtacc accgccgggg cctgccggtg 120

aaaatcgagc gagaattcga tgtgtggcgc ctcgttgagg attcggacgg gcagaagggc 180

tgggtccatc aggcaaccct ggtgggaacc cgcacgtttg tcataccggg actgcccccc 240

agggtgatgc ccagcaggcg ggccagccca ccgccaagga gactgacgtg a 291

<210> 36

<211> 96

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_719 (BcsY)

<400> 36

Met Thr Gly Leu Pro Leu Pro Arg Phe Ala Ala Phe Arg Ala Asp Glu

1 5 10 15

Val Asn Leu Arg Thr Gly Pro Gly Gln Arg Tyr Pro Ile Asp Trp Val

20 25 30

Tyr His Arg Arg Gly Leu Pro Val Lys Ile Glu Arg Glu Phe Asp Val

35 40 45

Trp Arg Leu Val Glu Asp Ser Asp Gly Gln Lys Gly Trp Val His Gln

50 55 60

Ala Thr Leu Val Gly Thr Arg Thr Phe Val Ile Pro Gly Leu Pro Pro

65 70 75 80

Arg Val Met Pro Ser Arg Arg Ala Ser Pro Pro Pro Arg Arg Leu Thr

85 90 95

<210> 37

<211> 267

<212> DNA

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_720 (BcsC II)

<400> 37

gtgattggcc gggccgatac ccgcatcgta ggccacgtga cggatgtggc cgatgcggcg 60

gatatcaagg gcgcggtcat gctgcatgcg gatgccagca ccacatcggc cgtggtggcc 120

gtgctgcggc ccggcgtggt gggcacgctg cgccagtgcc aggcgggcac gccgtggtgc 180

aaggtttcgg tcaagcagta cagtggctgg atcgagcgca gcgccatgtg ggggcttttg 240

ccgcaggagg tcatagcgcc ctcctga 267

<210> 38

<211> 88

<212> PRT

<213> artificial sequence

<220>

<223> Acetobacter xylinum IFO13693_720 (BcsC II)

<400> 38

Met Ile Gly Arg Ala Asp Thr Arg Ile Val Gly His Val Thr Asp Val

1 5 10 15

Ala Asp Ala Ala Asp Ile Lys Gly Ala Val Met Leu His Ala Asp Ala

20 25 30

Ser Thr Thr Ser Ala Val Val Ala Val Leu Arg Pro Gly Val Val Gly

35 40 45

Thr Leu Arg Gln Cys Gln Ala Gly Thr Pro Trp Cys Lys Val Ser Val

50 55 60

Lys Gln Tyr Ser Gly Trp Ile Glu Arg Ser Ala Met Trp Gly Leu Leu

65 70 75 80

Pro Gln Glu Val Ile Ala Pro Ser

85

Claims (13)

1. A bacterial cellulose producing strain KOSS15, characterized in that it has a accession number of KCCM12270P and is classified and namedKomagataeibacter rhaeticusAnd the above bacterial cellulose producing strain KOSS15 has cellulose producing ability.

2. A composition for the preparation of cellulose tablets, characterized in that it comprises a bacterial cellulose-producing strain KOSS15 with deposit number KCCM12270P or a culture of said strain.

3. A method for producing cellulose sheets, comprising the step of culturing a bacterial cellulose-producing strain KOSS15 having a accession number of KCCM12270P.

4. The method for producing cellulose sheet according to claim 3, wherein the bacterial cellulose producing strain KOSS15 is cultured in a medium comprising (a) honey or (b) a sugar source of glucose and fructose and proline.

5. The method according to claim 4, wherein the sugar source is 0.1 to 5% by volume of (a) honey or 0.1 to 5% by weight of (b) glucose and fructose.

6. The method for producing a cellulose sheet according to claim 4 or 5, wherein the ratio of 2:3 comprises the glucose and fructose in a weight ratio.

7. A method for producing a bacterial cellulose sheet of bacterial KOSS15 strain using bacterial cellulose, comprising:

a preculture step (a) of culturing the strain in a preculture medium containing a sugar source and cellulase with stirring;

a main culture step (b) of adding the preculture solution of the above step to a main culture medium containing a sugar source and proline, and culturing under stirring;

a sheet transformation culturing step (c) of adding the main culture solution of the above step to a medium containing a sugar source and proline to perform stationary culture;

the strain has a accession number of KCCM12270P.

8. The method for producing bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 according to claim 7, wherein the preculture medium comprises 0.1 to 10.0% by weight of yeast extract, 0.002 to 0.2% by weight of magnesium sulfate, 0.002 to 0.2% by weight of calcium chloride, 0.2 to 20.0% by weight of ethanol, 0.0005 to 0.05% by weight of cellulase or a combination thereof.

9. The method for producing bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 according to claim 7, wherein the main medium and the medium in the step (c) comprise 0.01 to 1.0% by weight of proline, 0.002 to 0.2% by weight of magnesium sulfate, 0.002 to 0.2% by weight of calcium chloride, 0.01 to 1.0% by weight of sodium acetate, 0.02 to 2.0% by weight of acetic acid or a combination thereof.

10. The method for producing a bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 according to any one of claims 7 to 9, wherein the sugar source is (a) honey or (b) glucose and fructose.

11. The method for producing bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 as claimed in claim 7, wherein said sugar source is 0.1 to 5% by volume of (a) honey or 0.1 to 5% by weight of (b) glucose and fructose.

12. The method for producing a bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 according to claim 10, wherein the ratio of 2:3 comprises the glucose and fructose in a weight ratio.

13. The method for producing bacterial cellulose sheet using bacterial cellulose producing strain KOSS15 according to claim 7, wherein in said preculture step (a), the agitation culture is performed at 100 to 200rpm for 24 to 72 hours.

Images