CN111681707B - Method for evaluating temperature and humidity state of growth environment of individual nursery pigs based on relative abundance of nasal eukaryotic microorganisms - Google Patents
Method for evaluating temperature and humidity state of growth environment of individual nursery pigs based on relative abundance of nasal eukaryotic microorganisms Download PDFInfo
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Abstract
The invention discloses a method for evaluating the temperature and humidity state of the growth environment of a nursery pig individual based on the relative abundance of eukaryotic microorganisms in nasal cavities, which is characterized in that the relative abundance of eukaryotic microorganisms in nasal cavities, belonging to the phylum, class, order, family, genus and species level of eukaryotic microorganisms in nursery pigs relative to total eukaryotic organisms in nasal cavities is quantitatively detected, and the temperature and humidity environment state of the growth of the nursery pig individual is evaluated by using the combination of the relative abundance.
Description
Technical Field
The invention belongs to the technical field of evaluation of pig breeding environments, and particularly relates to a method for evaluating the temperature and humidity state of a growing environment where a nursery pig individual is located based on the relative abundance of nasal eukaryotic microorganisms.
Background
The breeding environment is one of factors determining the breeding productivity of live pigs, the contribution rate to the breeding of the live pigs is up to 25%, and the improvement of the breeding environment is a necessary means for improving the breeding productivity of the live pigs. The temperature and humidity state is the most important part of the cultivation environment, and can be represented by a temperature and humidity index. The warm-wet environment can influence the pig breeding productivity from multiple angles, the immunity of the pigs is reduced under the warm-wet environment of cold stress and heat stress, the fat deposition of the pigs is reduced at high temperature, and the pigs in the non-comfortable environment warm-wet state area need to consume more energy to maintain constant body temperature, so that the feed conversion rate is reduced. The equipment of current regulation plant live pig breeds warm and humid environment has cascade fan cooling system and heat preservation lamp electric fan heater system, can adjust power and the heat-generating heat preservation lamp has been produced, and accurate breed concept also is put forward and accurate environmental control is part in the accurate breed.
The accurate evaluation of the pig raising environment is a prerequisite for precise environment control, and the temperature and humidity environment part of the environment evaluation in the current pig raising process is generally completed by a thermometer and a hygrometer. The worker uses a thermometer and a hygrometer to measure the temperature and the humidity of the pig house, then uses a temperature-humidity index calculation formula to calculate the temperature-humidity index and evaluate the temperature-humidity environment of the pig house by using the temperature-humidity index calculation formula, wherein the temperature-humidity index can be calculated by any two values of dry bulb temperature (Td, DEG C), wet bulb temperature (Tw, DEG C), dew point (Tdp, DEG C) and relative humidity (RH, DEG C), and one of the following calculation formulas is selected: thi=td+0.36tdp+41.2 or thi=0.81 td+ (0.99 Td-14.3) rh+46.3 or thi=0.72 (td+tw) +40.6. The method can judge the temperature and humidity environment state of the breeding house at a certain time point, but the temperature and humidity environment state of the live pigs in a period of time cannot be accurately evaluated. The temperature and humidity states of the breeding house generally change dynamically along with time, and the temperature and humidity states of different areas of the same animal house are different, so that the temperature and humidity indexes measured and calculated by the thermometer and the hygrometer at fixed points at fixed time are difficult to evaluate the temperature and humidity states of live pigs moving in certain areas within a period of time. The environmental temperature and humidity state affects the growth of eukaryotic microorganisms, which are different in different types and levels of eukaryotic microorganisms, the environmental temperature and humidity state affects the growth of eukaryotic microorganisms at a level, which are different in different types of eukaryotic microorganisms, the optimum temperature condition for the growth of Saccharomyces (Saccharomyces) is 20-30 ℃, fusarium (Fusarium) can grow at 1-39 ℃ but the optimum growth temperature is 25-30 ℃, the suitable growth temperature of Rhizopus (Rhizopus) is 30-37 ℃, and the heat resistance of Candida (Candida) is not strong. Studies have shown that in winter the northern area has room temperature with warm air that is too high, but low relative humidity causes a large number of pathogens to grow in the respiratory tract.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for evaluating the temperature and humidity state of the growth environment of a nursery pig individual based on the relative abundance of eukaryotic microorganisms in the nasal cavity.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows:
the method for evaluating the temperature and humidity state of the growing environment of the individual nursery pigs based on the relative abundance of the eukaryotic microorganisms in the nasal cavities is to quantitatively detect the relative abundance of the eukaryotic microorganisms in the nasal cavities of the nursery pigs relative to the total eukaryotic microorganisms in the nasal cavities at the same time and evaluate the temperature and humidity state of the growing environment of the nursery pigs by utilizing the relative abundance of the eukaryotic microorganisms in the nasal cavities or the combination of the eukaryotic microorganisms, wherein the eukaryotic microorganisms comprise the following steps: arthropoda phylum; liriopsis, saccharomyces, oligomembranous, chrysophyceae, chromophthora, phlebopus, hymenomycetes, pelaromycetes, tremella, pezizomyces, arachridae, coccomyces, monogonophyta, chlorophyceae, malasseziomycetes, cryptophyceae, trimerella, and Hydra; the bacteria may be selected from the group consisting of Poales, saccharomyces (Saccharomyces), dipterocarpales (Diploglaterida), hypocrea (Hypotrans), trichosporoales, neocimastigles (Trichosporoales), thermomyces (Lecanales), eurotiales (Eurotiales), brassales, rosales, chlamygdomonales, malassociales (Malasszides), solanales, melales (Filobalides), stobacilobrachia (Style), fagarales, cycloalgae (Sphaeromyces), arcellales (Arcellineida), tremellales (Tremellales), silk algae (Uroteiales), pylimiomonales, hymenoales (Hymenoales), hymenomyces, hymenochaetales (Rhizopus), phascales, hymenochaetales (Rhizopus, hymenochaetales), phascomycetes (Rhizopus, sporoteiales, hymenochaetales); saccharomyces, trichosporonaceae, cong Chike, malassziaceae, phafficetaceae, haliodaceae, mrakiaceae, lichtheimaceae, rhizophthlydaxeae; zea, monomonas (Kazachstania), broccoli (Ochromonas), cladosporium (Sarocladium), bromeliohrix, debaryomyces (Debaryomyces), malassezia (Malassezia), malus, rhizopus (Rhizopus), naganishia, candida (Candida), sterkiella, herminiella, spenchance, spenchancercaria (Distilla), tausonia, hycomycete (Hymenochaetes), hydnocarpus (Hydnocarpus), tetrahymena, oryza, trisporium (Trisporum), trisporum (Trisporum), hydramyces (Perdes), hypocrea (Trisporum), hypocrea (Rhizopus); zea_mays strain, kazachsania_tillus strain, tetrahymena_corlisis strain, rhabdidialides_humicola strain, blastocystis_sp.
The method comprises the following specific steps:
(1) Establishing and selecting a regression model (selection standard: R) of the relative abundance of nasal microorganisms and the environmental temperature and humidity index of the nursery pig reflecting the temperature and humidity environment state of the nursery pig 2 >0.8, requirement p relating to the p value<0.05 As follows:
(1) class level: thi= 77.04-12.85x 1 -82.96x 2 +38.29x 3 +93.41x 4 -8.59x 5 -562.97x 6 -853.91x 7 +772.03x 8 +1543.54x 9 -24754.73x 10 +1117.40x 11 -4959.84x 12 +6713.67x 13 +212386.71x 14 -3805.85x 15 +30401.80x 16 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.8613；
Wherein THI is the temperature and humidity index, x of the environment where the nursery pig is located 1 To x 16 The relative abundance of the eukaryotic microorganisms Liliopsis, saccharomyces, olibanum, jinzao, haemonchus, chaetomium, tremella, pantoea, arachnida, coglobophyceae, monognathus, chlorophyceae, malassezideziomycetes, cryptophyceae, phaeophyta, and Hydra relative to the total eukaryotic microorganisms in the nasal cavity, respectively, the relative abundance being in units of: the%;
(2) mesh level: thi=76.43-17.68 x 1 -125.78x 2 -245.52x 3 -669.06x 4 -1091.73x 5 +1195.59x 6 -4130.28x 7 +3832.18x 8 +5401.91x 9 -22665.39x 10 -1262.74x 11 +6273.57x 12 -1213.96x 13 +94177.67x 14 -8138.89x 15 +778026.7x 16 +4940.84x 17 +95728.04x 18 -20800.44x 19 -104152.25x 20 +72337.23x 21 -260723.7x 22 +1.47x 23 -44706.57x 24 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.8613；
Wherein THI is the temperature and humidity index, x of the environment where the nursery pig is located 1 To x 24 The plant is selected from the group consisting of a eukaryotic microorganism Poales, a yeast, a double stomach nematode, a meat-base, a Trichosporoales, a Neocilimnostoc, a tea stain, a Euglena, a Brassales, a Rosales, a Chromonas, a Margara, a Solanales, a Leucopia, a Stollobrachia, a Fagales, a Cycloalgae, a watchcase, a Yinychus, a Silk, a Pyramiodales, a rust, and a fungusThe relative abundance of the gram, basket spongiform, cystofilobasiales relative to total nasal eukaryotic microorganisms in units of: the%;
(3) family level: thi= 74.69403-118.91x 1 -114.10x 2 -293.41x 3 +577.06x 4 -718.67x 5 -130563.55x 6 -8165.30x 7 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.8301；
Wherein THI is the temperature and humidity index, x of the environment where the nursery pig is located 1 To x 7 For preserving the relative abundance of porcine nasal eukaryotic microorganisms of the family Saccharomyces, trichosporonaceae, combretaceae, malassziaceae, phafficetaceae, alternariaceae, mrakiaceae relative to total nasal eukaryotic microorganisms, the relative abundance is in units of: the%;
(4) the following levels: thi= 78.76-30.35x 1 -36.64x 2 +16.03x 3 -1109.55x 4 -2820.99x 5 -206.42x 6 +354.05x 7 -44705.11x 8 -22761.51x 9 +21032.98x 10 -35505.62x 11 -33058.75x 12 -27239.03x 13 -13604.5x 14 +165745.7x 15 -1.78x 16 -93578.76x 17 -41798.36x 18 -190048.3x 19 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.9861；
Wherein THI is the environmental temperature and humidity index, x of the nursery pig 1 To x 19 The relative abundance of the eukaryotic microorganisms Zea, monomonas (Kazachstania), bromelania (Ochromonas), cladosporium (Sarocladium), bromeliohrix, debaryomyces (Debaryomyces), malassezia (Malassezia), malus, rhizopus (Rhizopus), naganishia, candida, sterliella, hemiptrea, sprengertinella, rhizomucor (Rhizomucor), chlamydocarpus (Distigma), tausonia, hydnochaeta (Hydnochaete) with respect to the total eukaryotic microorganism, respectively, in terms of: the%;
(5) a level of: thi=77.30-26.08 x 1 -101.62x 2 +12.81x 3 -393.02x 4 +5037.59x 5 -2847.75x 6 -18478.26x 7 +42621.47x 8 -31045.96x 9 -49866.91x 10 -49842.29x 11 +7253.92x 12 -80561.46x 13 -17228.1x 14 -59962.73x 15 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.9741；
Wherein THI is the environmental temperature and humidity index, x of the nursery pig 1 To x 15 The relative abundance of the species Trebouxia jameii, nagania vishenicaci, diutan rugosa, hemitifera davidii, spencermartiella europaea, bahusakala longisis, distigma protein, tauusia puullans, hydnochaete duportii relative to the total eukaryotic microorganism in units of: the%;
(6) phylum, class, order, family, genus, species total level: thi=80.43+509.55x 1 -112454.19x 2 -150.30x 3 -27328.24x 4 +69.95x 5 +11819.60x 6 +292830.3x 7 +7566.22x 8 -657.14x 9 -246075.5x 10 +32726.74x 11 -5440.98x 12 -15061.11x 13 +16105.21x 14 -45.89x 15 +110.18x 16 -42.21x 17 +26.51x 18 -5.98x 19 -31.19x 20 -1325.87x 21 +782.44x 22 +108.99x 23 -40055.25x 24 -132290.1x 25 +1083.27x 26 +11120.24x 27 -44335.50x 28 +2783.59x 29 -29868.41x 30 -3012.42x 31 -137311.8x 32 +1149.58x 33 -31.01x 34 -3115.54x 35 -6.96x 36 -49597.7x 37 -3632.9x 38 -377.93x 39 -232.58x 40 -12.80x 41 +39827.06x 42 -1074.11x 43 +1585.58x 44 +30.82x 45 -1343.39x 46 -91516.32x 47 -198.74x 48 +59262.55x 49 -9029.64x 50 -197163.71x 51 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 ＝0.9925；
Wherein THI is the environmental temperature and humidity index, x of the nursery pig 1 To x 51 Is prepared from eukaryotic microorganism Arthropoda, ocular, chromonchonaceae, coccococcus, broccoflagellata, brassales, fagales, cycloalgae, rhizophalyctidales, eugleales, anthoate, silk algae, cystofilobasidiae, lichthemiaceae, rhizophalyctidaceae, unclassified Cycloalgae, zea, tetrahymena, oryza, mastosporium, scopulariella, hordeum, arabalis, cococci, malus, shengifera, naganishia, candida, aegilops, sterkella, etc the relative abundance of the species Hemiptelea, spencgertinsiella, rhizomucor, chlamydonella, pedoschalella, rhizochysolomyces, bacteroides, tausonia, rhabdiditides-humicolas, trichosporon, zea-mays.1, blastocystis sp. _subtype_3, trebouxia jameii, naganishia_vishinii, aegilops_tauchi, spenchanteriella_europaea, chlamydonella_irgularis, pedosphagostemium_enchymens, lasiosphaerius_apus 2010, distigma_protein, ammophilus sp, the relative abundance units are: the%;
(2) Quantitatively measuring the relative abundance of eukaryotic microorganisms in the nasal cavity of the nursery pig, introducing the measured result into a corresponding regression model, and calculating to obtain the environmental temperature and humidity index of the nursery pig in a short period.
Wherein the relative abundance index of the nasal eukaryotic microorganism is obtained based on 18S rDNA sequencing of an IonS5TMXL sequencing platform
The invention is further described below:
the diet and the variety in the breeding process of the nursery pigs are ensured to be consistent, the breath heat measuring cabin is used for ensuring that the pigs are in a stable environment temperature and humidity state and the design is repeated, and the fluctuation of the relative abundance of eukaryotic microorganisms in the nasal cavity is only influenced by the environment temperature and humidity state. The invention respectively carries out correlation analysis on the relative abundance index of each nasal cavity eukaryotic microorganism of the nursery pig under different temperature and humidity environment state test conditions and the temperature and humidity index of the corresponding environment, selects a nasal cavity eukaryotic microorganism single index with stronger correlation with the temperature and humidity environment state to build a model (selection standard: correlation analysis |r|)>0.6，p<0.05 The nasal cavity eukaryotic microorganism relative abundance index is comprehensively combined with the corresponding temperature and humidity index, and a model is established and selected by utilizing Matlab software (selection standard: r is R 2 >0.8, requirement p relating to the p value<0.05 The nasal cavity eukaryotic microorganism relative abundance index combination capable of accurately reflecting the temperature and humidity environment state is searched, the temperature and humidity environment state of the nursery pig in a period of time is evaluated by using the nasal cavity eukaryotic microorganism relative abundance index and is represented by a temperature and humidity index predicted value, and the temperature and humidity state evaluation of the nursery pig growth is more objective and reasonable.
The animal in the invention is a nursery pig, and can be popularized to other growth stages of live pigs and even to other animals and people, the method for detecting the relative abundance of the eukaryotic microorganisms in the nasal cavity is not unique, and other technical means capable of accurately detecting the relative abundance of the eukaryotic microorganisms in the nasal cavity can be replaced.
The single index and index combination of the relative abundance of other nursery pig nasal eukaryotic microorganisms not mentioned in the invention can be used for constructing a model through unitary or multiple regression analysis and environmental temperature and humidity index values; in the production stage of other pigs (fattening pigs, bred pigs and the like), a model can be built through single indexes and index combinations of eukaryotic microorganisms in the nasal cavities of the nursery pigs and environmental temperature and humidity index values through unitary or multiple regression analysis, and the model is used for evaluating the environmental temperature and humidity states; and are also within the scope of the present invention.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the temperature and humidity environment (expressed by temperature and humidity index values) of the nursery pig in a short time in the past can be accurately evaluated by measuring the relative abundance of the nasal eukaryotic microorganism corresponding to the nursery pig and using the model. The calculated temperature and humidity index of the environment where the nursery pig is located has guiding significance for a manager to adjust factors such as temperature, humidity and the like of the nursery pig house, and the temperature and humidity environment of the nursery pig can be controlled to be always in an optimal state in the production process by combining with the growth environment standard of the nursery pig.
Drawings
Fig. 1 shows the trend of the temperature and humidity index change during the test: the abscissa is time, the ordinate is temperature and humidity index value, the test is started from the first day 12:00 to the next day 6:00, the observation time interval is uniformly 30min (the temperature and humidity environment of the breathing cabin is in a stable state in the observation time period), the temperature and humidity index value in the graph is calculated by dry bulb temperature and relative humidity measured by a temperature sensor and a humidity sensor of the breathing cabin according to the formula THI=0.81 Td+ (0.99 Td-14.3) RH+46.3, and the temperature and humidity index value in a small frame in the graph is the average value in the whole test process (the average value of the temperature and humidity index values observed in the test time period is obtained).
Detailed Description
The experimental methods, materials and reagents used in the examples described below are conventional methods, materials and reagents, unless otherwise specified, and are commercially available.
1. Test animals
40 large-long-white binary hybrid nursery pigs with no significant weight difference are selected and randomly divided into 5 groups, 8 nursery pigs (n=8) in each group are fed into a respiratory cabin in a single column, and enough drinking water is supplied to provide the same reference NRC standard configuration feed for free feeding.
2. Test procedure and sample collection
The temperature and humidity environment of the nursery pigs in the breath heat measuring cabin is controlled, and the temperature and humidity environment of each group is the same, and is controlled by an air conditioner in the test. After the later calculation, the environmental temperature and humidity indexes of the growth of 5 groups of test pigs in the test period are 57.5, 62.1, 74.4, 81.8 and 83.7 respectively (the calculation process is detailed in the description of the drawings). Each test pig was raised in a stable warm and humid environment for about 20 hours, after which the nasal vestibule of the nursery pig was scraped using medical sterilized cotton to collect a nasal eukaryotic microorganism sample of the nursery pig.
3. Nasal cavity microorganism sample detection and data analysis
The 18S rDNA sequencing of the IonS5TMXL sequencing platform was used to detect the relative abundance of eukaryotic levels of nasal microbial samples. Utilizing IBM SPSS Statistics software to make correlation analysis on relative abundance of single nasal cavity eukaryotic microorganism and test temperature-humidity index, and selecting nasal cavity eukaryotic microorganism single index with strong correlation with temperature-humidity environment state to make model (selection standard: correlation analysis |r|)>0.6，p<0.05 Then, the nasal cavity eukaryotic microorganism relative abundance index and the corresponding temperature and humidity index are integrated, and a model is established and selected by Matlab software (selection standard: r is R 2 >0.8, requirement p relating to the p value<0.05 Searching for a blood metabolite index combination capable of accurately reflecting the temperature and humidity environment state.
4. Test results (see Table 1 and FIG. 1)
TABLE 1 temperature and humidity index regression equation of environment where nursery pigs are located is calculated by using eukaryotic microorganism indexes of nasal cavities of nursery pigs
The single index of the relative abundance of the eukaryotic microorganisms in the nasal cavity cannot establish a regression model which accords with the selection standard with the environmental temperature and humidity index, but the environmental temperature and humidity index of the nursery pig in a short period is estimated by utilizing the combination of the indexes of the eukaryotic microorganisms in the nasal cavity. The optimal model for evaluating the temperature and humidity state of the environment where the nursery pig grows by utilizing the nasal eukaryotic microorganism combination is as follows: thi=80.43+509.55x 1 -112454.19x 2 -150.30x 3 -27328.24x 4 +69.95x 5 +11819.60x 6 +292830.3x 7 +7566.22x 8 -657.14x 9 -246075.5x 10 +32726.74x 11 -5440.98x 12 -15061.11x 13 +16105.21x 14 -45.89x 15 +110.18x 16 -42.21x 17 +26.51x 18 -5.98x 19 -31.19x 20 -1325.87x 21 +782.44x 22 +108.99x 23 -40055.25x 24 -132290.1x 25 +1083.27x 26 +11120.24x 27 -44335.50x 28 +2783.59x 29 -29868.41x 30 -3012.42x 31 -137311.8x 32 +1149.58x 33 -31.01x 34 -3115.54x 35 -6.96x 36 -49597.7x 37 -3632.9x 38 -377.93x 39 -232.58x 40 -12.80x 41 +39827.06x 42 -1074.11x 43 +1585.58x 44 +30.82x 45 -1343.39x 46 -91516.32x 47 -198.74x 48 +59262.55x 49 -9029.64x 50 -197163.71x 51 (comprehensive of levels of phylum, class, order, family, genus, species, LASSO model, determination of coefficient R 2 =0.9925), wherein THI is the environmental temperature and humidity index of the nursery pig, x 1 To x 51 The eukaryotic microorganisms of the nasal cavity of the nursery pigs, arthropoda, euglena (Euglena), chromadorea (Chromadorea), coccococcus (Trebouxiophyceae), broccoflagellates (Ochromonadacles), brassica, fagales, cycloalgae (Sphaerales), rhizophalyctidales, euglena (Oygenales), anthateca, silk (Ulotricha), cystofilobariales, lichtheimaceae, rhizophalytydaceae, unclassified Cycloalgae (unidentified) of the genus Zea, tetrahymenales, oryza, trichosporon (Trichosporon) the genus Scopularium (Sarocladium), hordeum, arabidopsis, cococci (Trebouxia), malus, shensonia (Colpoda), naganishia, candida, aegizops, sterliella, hemiptelea, spencermartiella, rhizomucor (Rhizomucor), chlamydonella, pedospalella, rhizopus (Rhizophyctis), bacteroides (Distingma), tausonia, rhabdididosides_humicola, trichosporon (Trichosporon_coreformis), zosteroni_mays, zeastocystis_sp.The relative abundance (in%) of Trebouxia jameii species, naganishia vishenicacii species, aegilops tauschii species, spencertiella europaea species, chlamydonella irregularis species, pedosumella encystens species, lasioseius sp.
Claims (2)
1. A method for evaluating the warm and humid state of a growing environment in which a nursery pig individual is located based on the relative abundance of eukaryotic microorganisms in the nasal cavity, characterized in that the method quantitatively detects the relative abundance of eukaryotic microorganisms in the nasal cavity of the nursery pig in phylum, class, order, family, genus and species levels relative to the total eukaryotic organisms in the nasal cavity, and evaluates the warm and humid state of the growing environment in which the nursery pig individual grows by using the combination of the relative abundance thereof, the eukaryotic microorganisms comprising:Arthropodafungus door;Liliopsidathe bacteria and the yeast areSaccharomycetes) The oligomembrana isOligohymenophorea) The class of the JinzhenChrysophyceae) Ramulus et folium Haemonchi OleraceiChromadorea) The fecal crustacean classSordariomycetes) Tremella classTremellomycetes) The Pantoea isPezizomycetes）、ArachnidaThe bacteria and the coccoid algae areTrebouxiophyceae) The single nest classMonogononta) Green algae classChlorophyceae）、MalasseziomycetesThe fungus class and the Cryptophyceae classCryptophyceae) The class of class DSpirotrichea) Hydra classHydrozoa）；PoalesThe order of the fungus and the order of the microzymeSaccharomycetales) The double stomach nematode isDiplogasterida) The order of the sarcodactylisHypocreales）、TrichosporonalesBacterial mesh of,NeocallimastigalesBacterial eyes and tea stains eyesLecanorales) The order of EurotiumEurotiales）、BrassicalesBacterial mesh of,RosalesBacterial mesh of,ChlamydomonadalesThe order of the bacteria and the order of the malasseziaMalasseziales）、SolanalesThe order of the fungus and the order of the wire black powder fungusFilobasidiales）、StolidobranchiaBacterial mesh of,FagalesThe order of bacteria and the order of CycloalgaeSphaeropleales) The eye of the watchcaseArcellinida) The tremella is of the orderTremellales) The order of Siberian algaeUlotrichales）、PyramimonadalesBacterial order, hymenochaetales orderHymenochaetales) Sponge mesh of basketClathrinida）、CystofilobasidialesThe fungus and euglena are%Euglenida) The order of the Phaeophyllum palmatumOchromonadales）、RhizophlyctidalesBacterial mesh of,AnthoathecataThe strain of the genus Cycloalgae is not classifiedunidentified_Sphaeropleales) The order of EurotiumOnygenales) The method comprises the steps of carrying out a first treatment on the surface of the The Saccharomyces family isSaccharomycetaceae）、TrichosporonaceaeThe family of bacteria and Cong ChikeNectriaceae）、MalasseziaceaeThe fungus department,PhaffomycetaceaeThe fungus family and the Phanerochaete familyHymenochaetaceae）、MrakiaceaeThe fungus department,LichtheimiaceaeThe fungus department,RhizophlyctidaceaeThe family of bacteria;Zeagenus Saccharomyces, monomonas @Kazachstania) Genus ZosteraOchromonas) The broom branch mould belongs to the genusSarocladium）、BromeliothrixGenus Saccharomyces, debaryomycesDebaryomyces) Genus MalachitumMalassezia）、MalusGenus of fungus and rhizopusRhizopus）、NaganishiaGenus candidaCandida）、SterkiellaGenus of bacteria,HemipteleaGenus of bacteria,SpencermartinsiellaGenus Mortierella and rhizomucorRhizomucor）、ChlamydonellaGenus of fungus, genus of Bacteroides and genus of BacteroidesDistigma）、TausoniaGenus Phanerochaete and genus PhanerochaeteHydnochaete) Genus TetrahymenaTetrahymena）、OryzaGenus of fungus, genus of MaosporiumTrichosporon）、HordeumGenus of bacteria,ArabisGenus of fungus and reniform pestColpoda）、AegilopsGenus of bacteria,PedospumellaGenus of bacteria and genus of RhizopusRhizophlyctis) The co-chlorella isTrebouxia）；Zea_maysBacterial strain,Kazachstania_tellurisBacterial strain,Tetrahymena_corlissiBacterial strain,Rhabditidoides_ humicolusBacterial strain,Blastocystis_sp._subtype_3Strain and candida albicansCandida_albicans）、Trebouxia_jamesiiBacterial strain,Naganishia_vishniaciiBacterial strain,Diutina_rugosaBacterial strain,Hemiptelea_davidiiBacterial strain,Spencermartinsiella_europaeaBacterial strain,Bahusakala_longisporaBacterial strain,Distigma_proteusBacterial strain,Tausonia_pullulansBacterial strain,Hydnochaete_duportiiStrain and candidaTrichosporon_coremiiforme）、Zea_mays.1Bacterial strain,Aegilops_tauschiiBacterial strain,Chlamydonella_irregularisBacterial strain,Pedospumella_encystansBacterial strain,Lasioseius_sp._ APGD.2010Bacterial strain,Ammopiptanthus_mongolicusA strain; the method comprises the following specific steps:
(1) The regression model of the relative abundance of the nasal microorganisms and the environmental temperature and humidity index of the nursery pig reflecting the temperature and humidity environment state of the nursery pig is established and selected as follows:
class level: thi= 77.04-12.85x 1 -82.96x 2 +38.29x 3 +93.41x 4 -8.59x 5 -562.97x 6 -853.91x 7 +772.03x 8 +1543.54x 9 -24754.73x 10 +1117.40x 11 -4959.84x 12 +6713.67x 13 +212386.71x 14 -3805.85x 15 +30401.80x 16 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.8613；
Wherein THI is the temperature and humidity index of the environment where the nursery pig is located,x 1 to the point ofx 16 Eukaryotic microorganisms for nasal cavities of nursery pigs respectivelyLiliopsidaThe fungus class, the yeast class, the oligomembrana class, the golden algae class, the color spear class, the fecal chitosanase class, the tremella class, the tray fungus class,ArachnidaThe bacteria, the coccoid algae, the unicellular algae, the green algae,MalasseziomycetesThe relative abundance of the bacterial, cryptophycin, trichina, hydroids relative to total nasal eukaryotic microorganisms in units of: the%;
mesh level: thi=76.43-17.68x 1 -125.78x 2 -245.52x 3 -669.06x 4 -1091.73x 5 +1195.59x 6 -4130.28x 7 +3832.18x 8 +5401.91x 9 -22665.39x 10 -1262.74x 11 +6273.57x 12 -1213.96x 13 +94177.67x 14 -8138.89x 15 +778026.7x 16 +4940.84x 17 +95728.04x 18 -20800.44x 19 -104152.25x 20 +72337.23x 21 -260723.7x 22 +1.47x 23 -44706.57x 24 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.8613；
Wherein THI is the temperature and humidity index of the environment where the nursery pig is located,x 1 to the point ofx 24 Eukaryotic microorganisms for nasal cavities of nursery pigs respectivelyPoalesThe order of the genus Saccharomyces, the order of the genus Digonematoda, the order of the genus Sarcophaga,TrichosporonalesBacterial mesh of,NeocallimastigalesBacteria, tea stain, cyst, bacteria,BrassicalesBacterial mesh of,RosalesBacterial mesh of,ChlamydomonadalesThe order of bacteria, the order of malassezia, the order of bacteria,SolanalesThe order of the fungus, the order of the line black powder fungus,StolidobranchiaBacterial mesh of,FagalesBacteria, cycloalgae, watchcase, silver ear, silk algae,PyramimonadalesThe fungus mesh, the rust leather pore fungus mesh, the basket sponge mesh,CystofilobasidialesThe relative abundance of the bacterial mesh relative to the total nasal cavity eukaryotic microorganism, the relative abundance being in units of: the%;
family level: thi= 74.69403-118.91x 1 -114.10x 2 -293.41x 3 +577.06x 4 -718.67x 5 -130563.55x 6 -8165.30x 7 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.8301；
Wherein THI is the temperature and humidity index of the environment where the nursery pig is located,x 1 to the point ofx 7 Is a eukaryotic microorganism of the nasal cavity of a nursery pig, a microzyme family,TrichosporonaceaeThe family of bacteria, the family of Confucius,MalasseziaceaeThe fungus department,PhaffomycetaceaeThe fungus family, the Phanerochaete family,MrakiaceaeTotal eukaryotic micro-organisms of the family Juncology relative to the nasal cavityA relative abundance of an organism, the relative abundance being in units of: the%;
the following levels: thi= 78.76-30.35x 1 -36.64x 2 +16.03x 3 -1109.55x 4 -2820.99x 5 -206.42x 6 +354.05x 7 -44705.11x 8 -22761.51x 9 +21032.98x 10 -35505.62x 11 -33058.75x 12 -27239.03x 13 -13604.5x 14 +165745.7x 15- 1.78x 16 -93578.76x 17 -41798.36x 18 -190048.3x 19 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.9861；
Wherein THI is the environmental temperature and humidity index of the nursery pig,x 1 to the point ofx 19 Eukaryotic microorganisms for nasal cavities of nursery pigs respectivelyZeaGenus Saccharomyces, monomonas @Kazachstania) Genus ZosteraOchromonas) The broom branch mould belongs to the genusSarocladium）、BromeliothrixGenus Saccharomyces, debaryomycesDebaryomyces) Genus MalachitumMalassezia）、MalusGenus of fungus and rhizopusRhizopus）、NaganishiaGenus candidaCandida）、SterkiellaGenus of bacteria,HemipteleaGenus of bacteria,SpencermartinsiellaGenus Mortierella and rhizomucorRhizomucor）、ChlamydonellaGenus of fungus, genus of Bacteroides and genus of BacteroidesDistigma）、TausoniaGenus Phanerochaete and genus PhanerochaeteHydnochaete) Relative abundance of total eukaryotic microorganisms relative to nasal cavity, the relative abundance being in units of: the%;
a level of: thi=77.30-26.08x 1 -101.62x 2 +12.81x 3 -393.02x 4 +5037.59x 5 -2847.75x 6 -18478.26x 7 +42621.47x 8 -31045.96x 9 -49866.91x 10 -49842.29x 11 +7253.92x 12 -80561.46x 13 -17228.1x 14 -59962.73x 15 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.9741；
Wherein THI is the environmental temperature and humidity index, x of the nursery pig 1 To x 15 Eukaryotic microorganisms for nasal cavities of nursery pigs respectivelyZea_maysBacterial strain,Kazachstania_tellurisBacterial strain,Tetrahymena_corlissiBacterial strain,Rhabditidoides_ humicolusBacterial strain,Blastocystis_sp._subtype_3Bacterial strain, candida albicans,Trebouxia_jamesiiBacterial strain,Naganishia_vishniaciiBacterial strain,Diutina_rugosaBacterial strain,Hemiptelea_davidiiBacterial strain,Spencermartinsiella_europaeaBacterial strain,Bahusakala_longisporaBacterial strain,Distigma_proteusBacterial strain,Tausonia_pullulansBacterial strain,Hydnochaete_duportiiThe relative abundance of bacterial species relative to total nasal eukaryotic microorganisms, the relative abundance in units of: the%;
phylum, class, order, family, genus, species total level: thi=80.43+509.55x 1 -112454.19x 2 -150.30x 3 -27328.24x 4 +69.95x 5 +11819.60x 6 +292830.3x 7 +7566.22x 8 -657.14x 9 -246075.5x 10 +32726.74x 11 -5440.98x 12 -15061.11x 13 +16105.21x 14 -45.89x 15 +110.18x 16 -42.21x 17 +26.51x 18 -5.98x 19 -31.19x 20 -1325.87x 21 +782.44x 22 +108.99x 23 -40055.25x 24 -132290.1x 25 +1083.27x 26 +11120.24x 27 -44335.50x 28 +2783.59x 29 -29868.41x 30 -3012.42x 31 -137311.8x 32 +1149.58x 33 -31.01x 34 -3115.54x 35 -6.96x 36 -49597.7x 37 -3632.9x 38 -377.93x 39 -232.58x 40 -12.80x 41 +39827.06x 42 -1074.11x 43 +1585.58x 44 +30.82x 45 -1343.39x 46 -91516.32x 47 -198.74x 48 +59262.55x 49 -9029.64x 50 -197163.71x 51 The method comprises the steps of carrying out a first treatment on the surface of the The model is LASSO model, and the coefficient R is determined 2 =0.9925；
Wherein THI is the environmental temperature and humidity index of the nursery pig,x 1 to the point ofx 51 Is eukaryotic microorganism for preserving the nasal cavity of pigsArthropodaThe plant is selected from the group consisting of Eumycota, chromoclass Coccolobata, palstochaeta, and Palstochaeta,BrassicalesBacterial mesh of,FagalesThe order of bacteria, the order of Cycloalgae, and the order of,RhizophlyctidalesThe fungus mesh, the Eumycota,AnthoathecataThe order of bacteria, silk algae, etc,CystofilobasidialesBacterial mesh of,LichtheimiaceaeThe fungus department,RhizophlyctidaceaeThe fungus family, the order of the unclassified Cycloalgae,ZeaGenus bacteria, tetrahymena, genus bacteria,OryzaGenus Mortierella, genus Scorpion,HordeumGenus of bacteria,ArabisGenus of bacteria, genus of co-chlorella,MalusGenus Bacillus, genus reniform pest,NaganishiaGenus Candida, genus Candida,AegilopsGenus of bacteria,SterkiellaGenus of bacteria,HemipteleaGenus of bacteria,SpencermartinsiellaGenus Mortierella, rhizomucor,ChlamydonellaGenus of bacteria,PedospumellaGenus Bacillus, rhizopus, bacteroides, and Sphaeroides,TausoniaGenus of bacteria,Rhabditidoides_humicolusBacterial strain, candida,Zea_mays.1Bacterial strain,Blastocystis_sp._subtype_3Bacterial strain,Trebouxia_jamesiiBacterial strain,Naganishia_vishniaciiBacterial strain,Aegilops_tauschiiBacterial strain,Spencermartinsiella_europaeaBacterial strain,Chlamydonella_ irregularisBacterial strain,Pedospumella_encystansBacterial strain,Lasioseius_sp._APGD.2010Bacterial strain,Distigma_proteusBacterial strain,Ammopiptanthus_mongolicusThe relative abundance of bacterial species relative to total nasal eukaryotic microorganisms, the relative abundance in units of: the%;
(2) Quantitatively measuring the relative abundance of eukaryotic microorganisms in the nasal cavity of the nursery pig, introducing the measured result into a corresponding regression model, and calculating to obtain the environmental temperature and humidity index of the nursery pig in a short period.
2. The method of claim 1, wherein the nasal eukaryotic microorganism relative abundance index is obtained based on 18S rDNA sequencing of an ion S5TMXL sequencing platform.
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