CN111671015A - Feed additive for improving digestion and absorption functions of fishes - Google Patents
Feed additive for improving digestion and absorption functions of fishes Download PDFInfo
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- CN111671015A CN111671015A CN202010577854.1A CN202010577854A CN111671015A CN 111671015 A CN111671015 A CN 111671015A CN 202010577854 A CN202010577854 A CN 202010577854A CN 111671015 A CN111671015 A CN 111671015A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Insects & Arthropods (AREA)
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Abstract
The invention provides a feed additive for improving the digestion and absorption functions of fishes, which is prepared from the following raw materials in parts by weight: 0.1-0.5 part of akebia stem phenethyl alcohol B, 10-15 parts of corn stigma extract, 15-30 parts of dry brewer's grains, 1-5 parts of soybean lecithin, 1-10 parts of magnesium oxide and 1-5 parts of potassium diformate. The feed additive for improving the digestion and absorption functions of the fishes can effectively improve the digestion and absorption functions of the fishes by adding the soybean lecithin, the akebia stem phenethyl alcohol B, the corn stigma extract, the dry brewer's grains, the magnesium oxide and the potassium diformate.
Description
Technical Field
The invention belongs to the field of fish culture, and particularly relates to a feed additive method for improving the digestion and absorption functions of fish.
Background
With the continuous improvement of living standard of people, the consumption of aquatic products such as fish and the like is also continuously increased. The fish meal and the bean pulp are high-quality protein sources of aquaculture animals. In recent years, with the rapid development of aquaculture industry, the feed demand increases year by year, so that the supply and demand of high-quality protein sources are tense, the price is high, and the sustainable development of aquaculture industry is severely restricted. The searching of protein sources with high quality, low price and rich sources becomes an important way for solving the problem.
Disclosure of Invention
In view of the above, the present invention provides a feed additive for improving the digestive absorption function of fish, which overcomes the defects in the prior art.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
a feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight:
preferably, the additive is prepared from the following raw materials in parts by weight:
preferably, the additive is prepared from the following raw materials in parts by weight:
a preparation method of a corn stigma extract comprises the following steps:
(1) drying corn stigma in the air, crushing, heating the obtained powder particles for 1-2h, and cooling to room temperature to obtain a crude product;
(2) adding distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment, and adding an enzyme preparation into the mixture after ultrasonic treatment for enzymolysis;
(3) concentrating the liquid obtained after enzymolysis, freeze-drying to obtain concentrated powder, pulverizing the obtained solid into powder, and mixing with the concentrated powder to obtain the corn stigma extract.
Further, the temperature of the heating step in the step (1) is 35-50 ℃.
Further, the mass ratio of the crude product in the step (2) to the distilled water is 1: 20-40; the ultrasonic treatment in the step (2) is carried out for 1-3 hours at a power of 50-60W.
Further, the temperature of the enzymolysis step in the step (2) is 40-50 ℃, and the enzymolysis time is 1-2 days; the enzyme preparation in the step (2) is a combination of cellulase, hemicellulase and xylanase.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean lecithin, adding akebia quinata phenethyl alcohol B, corn stigma extract, fermented brewer grains, magnesium oxide and potassium diformate, and uniformly mixing to obtain a premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
Further, the heating temperature in the step (1) is 50-65 ℃, and the heating time is 3-5 hours.
A feed for improving the digestion and absorption functions of fish comprises the additive.
Compared with the prior art, the invention has the following advantages:
the feed additive for improving the digestion and absorption functions of the fishes can effectively improve the digestion and absorption functions of the fishes by adding the soybean phospholipid, the akebia stem phenethyl alcohol B, the corn stigma extract, the dry brewer's grains, the magnesium oxide and the potassium diformate.
The corn stigma extract is obtained by heating, ultrasonic water extraction, enzymolysis and other methods, the heating enables substances such as saccharides, phytosterol, saponin and the like in the corn stigma to effectively overflow, the subsequent enzymolysis efficiency is improved, cellulase and xylanase are added in the enzymolysis process, a large amount of xylan and various glucose can be obtained, and the xylan is combined with other components, so that the digestion and absorption functions of fish can be improved.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The cellulase of the embodiment of the invention is purchased from Beijing Solaibao science and technology Limited; hemicellulase was purchased from Shanghai enzyme-linked Biotechnology, Inc.; xylanase was purchased from Shandong Zea Biotech Ltd.
The present invention will be described in detail with reference to examples.
Example 1
A feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight: 0.2 part of akebia stem phenethyl alcohol B, 12 parts of corn stigma extract, 20 parts of dry brewer's grains, 2 parts of soybean lecithin, 1 part of magnesium oxide and 1 part of potassium diformate.
A preparation method of a corn stigma extract comprises the following steps:
(1) pulverizing stigma Maydis into 0.1-0.3cm granules, heating the obtained granules at 40 deg.C for 2 hr, and cooling to room temperature to obtain crude product;
(2) adding 20 times of distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment at 60W for 2 hours, adding 20FPU/g of cellulase, 5U/mg of hemicellulase and 1000IU/g of xylanase into the mixture after ultrasonic treatment, performing enzymolysis at 40 ℃ for 1 day, and performing solid-liquid separation after enzymolysis;
(3) concentrating the liquid obtained in the step (2), freeze-drying to obtain concentrated powder, crushing the obtained solid into powder, and uniformly mixing with the concentrated powder to obtain the corn stigma extract.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean phospholipid at 60 deg.C for 3 hr, adding caulis Akebiae phenethyl alcohol B, stigma Maydis extract, fermented brewer's grains, magnesium oxide, and potassium diformate, and mixing to obtain premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
A feed for improving the digestion and absorption functions of fish comprises the additive.
Example 2
A feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight: 0.5 part of akebia stem phenethyl alcohol B, 12 parts of corn stigma extract, 20 parts of dry brewer's grains, 2 parts of soybean lecithin, 1 part of magnesium oxide and 1 part of potassium diformate.
A preparation method of a corn stigma extract comprises the following steps:
(1) pulverizing stigma Maydis into 0.1-0.3cm granules, heating the obtained granules at 40 deg.C for 2 hr, and cooling to room temperature to obtain crude product;
(2) adding 20 times of distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment at 60W for 2 hours, adding 20FPU/g of cellulase, 5U/mg of hemicellulase and 1000IU/g of xylanase into the mixture after ultrasonic treatment, performing enzymolysis at 40 ℃ for 1 day, and performing solid-liquid separation after enzymolysis;
(3) concentrating the liquid obtained in the step (2), freeze-drying to obtain concentrated powder, crushing the obtained solid into powder, and uniformly mixing with the concentrated powder to obtain the corn stigma extract.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean phospholipid at 60 deg.C for 3 hr, adding caulis Akebiae phenethyl alcohol B, stigma Maydis extract, fermented brewer's grains, magnesium oxide, and potassium diformate, and mixing to obtain premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
A feed for improving the digestion and absorption functions of fish comprises the additive.
Example 3
A feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight: 0.2 part of akebia stem phenethyl alcohol B, 15 parts of corn stigma extract, 20 parts of dry brewer's grains, 2 parts of soybean lecithin, 1 part of magnesium oxide and 1 part of potassium diformate.
A preparation method of a corn stigma extract comprises the following steps:
(1) pulverizing stigma Maydis into 0.1-0.3cm granules, heating the obtained granules at 40 deg.C for 2 hr, and cooling to room temperature to obtain crude product;
(2) adding 20 times of distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment at 60W for 2 hours, adding 20FPU/g of cellulase, 5U/mg of hemicellulase and 1000IU/g of xylanase into the mixture after ultrasonic treatment, performing enzymolysis at 40 ℃ for 1 day, and performing solid-liquid separation after enzymolysis;
(3) concentrating the liquid obtained in the step (2), freeze-drying to obtain concentrated powder, crushing the obtained solid into powder, and uniformly mixing with the concentrated powder to obtain the corn stigma extract.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean phospholipid at 60 deg.C for 3 hr, adding caulis Akebiae phenethyl alcohol B, stigma Maydis extract, fermented brewer's grains, magnesium oxide, and potassium diformate, and mixing to obtain premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
A feed for improving the digestion and absorption functions of fish comprises the additive.
Example 4
A feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight: 0.2 part of akebia stem phenethyl alcohol B, 12 parts of corn stigma extract, 30 parts of dry brewer's grains, 2 parts of soybean lecithin, 1 part of magnesium oxide and 1 part of potassium diformate.
A preparation method of a corn stigma extract comprises the following steps:
(1) pulverizing stigma Maydis into 0.1-0.3cm granules, heating the obtained granules at 40 deg.C for 2 hr, and cooling to room temperature to obtain crude product;
(2) adding 20 times of distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment at 60W for 2 hours, adding 20FPU/g of cellulase, 5U/mg of hemicellulase and 1000IU/g of xylanase into the mixture after ultrasonic treatment, performing enzymolysis at 40 ℃ for 1 day, and performing solid-liquid separation after enzymolysis;
(3) concentrating the liquid obtained in the step (2), freeze-drying to obtain concentrated powder, crushing the obtained solid into powder, and uniformly mixing with the concentrated powder to obtain the corn stigma extract.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean phospholipid at 60 deg.C for 3 hr, adding caulis Akebiae phenethyl alcohol B, stigma Maydis extract, fermented brewer's grains, magnesium oxide, and potassium diformate, and mixing to obtain premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
A feed for improving the digestion and absorption functions of fish comprises the additive.
Example 5
A feed additive for improving the digestion and absorption functions of fishes is prepared from the following raw materials in parts by weight: 0.2 part of akebia stem phenethyl alcohol B, 12 parts of corn stigma extract, 20 parts of dry brewer's grains, 2 parts of soybean lecithin, 4 parts of magnesium oxide and 4 parts of potassium diformate.
A preparation method of a corn stigma extract comprises the following steps:
(1) pulverizing stigma Maydis into 0.1-0.3cm granules, heating the obtained granules at 40 deg.C for 2 hr, and cooling to room temperature to obtain crude product;
(2) adding 20 times of distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment at 60W for 2 hours, adding 20FPU/g of cellulase, 5U/mg of hemicellulase and 1000IU/g of xylanase into the mixture after ultrasonic treatment, performing enzymolysis at 40 ℃ for 1 day, and performing solid-liquid separation after enzymolysis;
(3) concentrating the liquid obtained in the step (2), freeze-drying to obtain concentrated powder, crushing the obtained solid into powder, and uniformly mixing with the concentrated powder to obtain the corn stigma extract.
The preparation method of the feed additive for improving the digestion and absorption functions of the fishes comprises the following steps: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean phospholipid at 60 deg.C for 3 hr, adding caulis Akebiae phenethyl alcohol B, stigma Maydis extract, fermented brewer's grains, magnesium oxide, and potassium diformate, and mixing to obtain premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
A feed for improving the digestion and absorption functions of fish comprises the additive.
The experiments of examples 1 to 5 were carried out, 300 normal-developed chubs were selected and subjected to a 90-day cultivation experiment, and the average was divided into 6 groups (the last group was used as a parallel group), and the additives obtained in examples 1 to 5 were added to the first 5 groups together with the feed, and the feed was given to fish culture with water to measure apparent digestibility, and the results are shown in table 1.
As shown in Table 1, the additives of examples 1 to 5 are effective in improving the apparent digestibility of chubs compared with the control group, wherein the additive of example 3 has the most obvious effect; it can be seen that by increasing the addition of the corn stigma extract and the dry brewer's grain, the digestion and absorption functions of the silver carp are both improved, the fermented brewer's grain increases beneficial bacteria, and plays a role in promoting digestion, and saccharides, phytosterols, saponins and other substances in the corn stigma extract can bring certain effects for improving digestion, and in example 4, the addition of akebia ethanol B is increased, and the addition of the corn stigma extract, magnesium and potassium are combined, so that the metabolism level of each organ is improved, and the digestion and absorption functions of the silver carp are effectively improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A feed additive for improving the digestion and absorption functions of fishes is characterized in that: the additive is prepared from the following raw materials in parts by weight:
2. the feed additive for improving digestive absorption function of fish according to claim 1, wherein: the additive is prepared from the following raw materials in parts by weight:
3. the feed additive for improving digestive absorption function of fish according to claim 2, wherein: the additive is prepared from the following raw materials in parts by weight:
4. a preparation method of a corn stigma extract is characterized by comprising the following steps: the method comprises the following steps:
(1) drying corn stigma in the air, crushing, heating the obtained powder particles for 1-2h, and cooling to room temperature to obtain a crude product;
(2) adding distilled water into the crude product obtained in the step (1), uniformly mixing, performing ultrasonic treatment, and adding an enzyme preparation into the mixture after ultrasonic treatment for enzymolysis;
(3) concentrating the liquid obtained after enzymolysis, freeze-drying to obtain concentrated powder, pulverizing the obtained solid into powder, and mixing with the concentrated powder to obtain the stigma Maydis extract.
5. The method for preparing corn silk extract according to claim 4, wherein: the temperature of the heating step in the step (1) is 35-50 ℃.
6. The method for preparing corn silk extract according to claim 4, wherein: the mass ratio of the crude product in the step (2) to the distilled water is 1: 20-40; the ultrasonic treatment in the step (2) is carried out for 1-3 hours at a power of 50-60W.
7. The method for preparing corn silk extract according to claim 4, wherein: the temperature of the enzymolysis step in the step (2) is 40-50 ℃, and the enzymolysis time is 1-2 days; the enzyme preparation in the step (2) is a combination of cellulase, hemicellulase and xylanase.
8. A method for producing a feed additive for improving digestive absorption function of fishes according to any one of claims 1 to 3, comprising: the method comprises the following steps:
(1) adding microorganisms into the dry brewer's grains for fermentation to obtain fermented brewer's grains;
(2) heating soybean lecithin, adding akebia quinata phenethyl alcohol B, a corn stigma extract, fermented brewer grains, magnesium oxide and potassium diformate, and uniformly mixing to obtain a premix;
(3) the feed additive for improving the digestion and absorption functions of the fishes is obtained by preparing the premix into a capsule form.
9. The method for producing a feed additive for improving digestive absorption function of fish according to claim 8, wherein: the heating temperature in the step (1) is 50-65 ℃, and the heating time is 3-5 hours.
10. A feed for improving the digestion and absorption functions of fishes is characterized in that: comprising the additive of any one of claims 1-3.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010577854.1A CN111671015A (en) | 2020-06-23 | 2020-06-23 | Feed additive for improving digestion and absorption functions of fishes |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010577854.1A CN111671015A (en) | 2020-06-23 | 2020-06-23 | Feed additive for improving digestion and absorption functions of fishes |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1589644A (en) * | 2003-09-06 | 2005-03-09 | 邱芳龙 | Application of extract of active component of plant in animal feedstuff |
| CN103598444A (en) * | 2013-10-14 | 2014-02-26 | 高雷 | Feed for tilapia mossambica |
| CN104222672A (en) * | 2014-09-28 | 2014-12-24 | 浙江海洋学院 | Turbot fry feed |
-
2020
- 2020-06-23 CN CN202010577854.1A patent/CN111671015A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1589644A (en) * | 2003-09-06 | 2005-03-09 | 邱芳龙 | Application of extract of active component of plant in animal feedstuff |
| CN103598444A (en) * | 2013-10-14 | 2014-02-26 | 高雷 | Feed for tilapia mossambica |
| CN104222672A (en) * | 2014-09-28 | 2014-12-24 | 浙江海洋学院 | Turbot fry feed |
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Application publication date: 20200918 |