CN111670915A - Microbial pesticide capable of being stored for long time in saline-alkali soil - Google Patents
Microbial pesticide capable of being stored for long time in saline-alkali soil Download PDFInfo
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- CN111670915A CN111670915A CN202010701731.4A CN202010701731A CN111670915A CN 111670915 A CN111670915 A CN 111670915A CN 202010701731 A CN202010701731 A CN 202010701731A CN 111670915 A CN111670915 A CN 111670915A
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- saline
- alkali soil
- microbial pesticide
- long time
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/34—Aspergillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a microbial pesticide for a long-term storage saline-alkali soil, which comprises the following components in percentage by weight: 3-8% of titanium dioxide, 85-90% of thallus dry matter with the effect of inhibiting biological germs and the balance of cattail wool powder. The microbial pesticide for saline-alkali soil has obvious control effect on various virus diseases of tobacco mosaic virus, cucumber mosaic virus, rice stripe virus and the like of various host plants, and particularly has very good control effect on pseudomonas syringae (pseudomonas syringae) causing wheat bacterial leaf blight. The microbial pesticide for the saline-alkali soil, which can be preserved for a long time, mainly comprises the microbial agent, inorganic salt and natural plant extract, does not contain organic pesticide components harmful to human bodies, is a safe and pollution-free pesticide, and can effectively prevent plant virus diseases even if being preserved for more than 3 years at normal temperature.
Description
Technical Field
The invention relates to the technical field of microbial pesticides, in particular to a microbial pesticide for saline-alkali soil, which can be stored for a long time.
Background
Microbial fertilizers are also called biological fertilizers, inoculants or bacterial fertilizers (bacterial manures) and the like, and refer to a fertilizer product which takes the life activities of microorganisms as the core and enables crops to obtain specific fertilizer effects. The microbial fertilizer and the micro-fertilizer have essential differences: the former are living life and the latter are mineral elements. The microbial resources are rich, the variety and the functions are various, and the fertilizer can be developed into fertilizers with different functions and different purposes. And the microbial strains can be artificially bred, continuously purified and rejuvenated to improve the activity of the strains, and particularly, with the further development of biotechnology, the acquisition of the required strains by a genetic engineering method becomes possible.
Microbial fertilizers are of many types, and microbial fertilizer products are generally divided into two main categories: one is a microbial fertilizer in the narrow sense, which means that the supply of plant nutrient elements is increased through the life activities of microorganisms, and comprises the steps of increasing the total supply of the plant nutrient elements in soil and production environment, so that the nutrient condition of the plants is improved, and the yield is increased, wherein the representative variety of the microbial fertilizer is rhizobium fertilizer; the other is a broad-sense microbial fertilizer, which means that the supply of plant nutrient elements can be improved through the life activities of microorganisms in the microbial fertilizer, plant growth hormone can be generated, the absorption and utilization of the nutrient elements by plants are promoted or the pathogenic effect of certain pathogenic microorganisms is antagonized, the crop diseases and insect pests are reduced, and the crop yield is increased.
However, as the main functional component in the microbial pesticide is the microbial agent which is relatively harsh to store, the shelf life of the general microbial pesticide is short, otherwise, the pesticide effect is influenced, and the microbial pesticide for the saline-alkali soil can be stored for a long time.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a microbial pesticide for saline-alkali soil, which can be stored for a long time.
The technical scheme of the invention is as follows:
a microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components: titanium dioxide, thalli dry matter with the effect of inhibiting biological germs, salt and alkali resistant thalli dry matter and cattail wool powder.
Preferably, the microbial pesticide for the saline-alkali soil capable of being stored for a long time consists of the following components in percentage by weight: 3-8% of titanium dioxide, 85-90% of thallus dry matter with the effect of inhibiting biological germs, 0.5-2% of saline-alkali resistant thallus dry matter and the balance of cattail wool powder.
Preferably, the cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
Preferably, the bacterial cells with the biological pathogen inhibition effect can be bacterial strains with the biological pathogen inhibition effect or mutant strains of the bacterial strains with the biological pathogen inhibition effect.
Preferably, the dried bacterial cell with the effect of inhibiting biological germs is a dried bacterial cell of Brevibacillus laterosporus.
Further preferably, the brevibacillus laterosporus is brevibacillus laterosporus SNB10, and the strain is deposited in China general microbiological culture Collection center on 5-17 th month in 2017 with the deposition numbers: 14171.
the invention has the advantages that: the microbial pesticide for the saline-alkali soil, which can be preserved for a long time, disclosed by the invention comprises the following components in percentage by weight: 3-8% of titanium dioxide, 85-90% of thallus dry matter with the effect of inhibiting biological germs and the balance of cattail wool powder. The microbial pesticide for saline-alkali soil has obvious control effect on various virus diseases of tobacco mosaic virus, cucumber mosaic virus, rice stripe virus and the like of various host plants, and particularly has very good control effect on pseudomonas syringae (pseudomonas syringae) causing wheat bacterial leaf blight. The microbial pesticide for the saline-alkali soil, which can be preserved for a long time, mainly comprises the microbial agent, inorganic salt and natural plant extract, does not contain organic pesticide components harmful to human bodies, is a safe and pollution-free pesticide, and can effectively prevent plant virus diseases even if being preserved for more than 3 years at normal temperature.
Detailed Description
Example 1
A microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components in percentage by weight: 5% of titanium dioxide, 88% of thallus desiccate with the effect of inhibiting biological germs, 1.2% of saline-alkali resistant thallus desiccate and the balance of cattail wool powder.
The cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
Example 2
A microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components in percentage by weight: 8% of titanium dioxide, 85% of thallus dry matter with the effect of inhibiting biological germs, 2% of saline-alkali resistant thallus dry matter and the balance of cattail wool powder.
The cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
Example 3
A microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components in percentage by weight: 3% of titanium dioxide, 90% of thallus dry product with the effect of inhibiting biological germs, 0.5% of saline-alkali resistant thallus dry product and the balance of cattail wool powder.
The cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
Example 4
A microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components in percentage by weight: titanium dioxide 8%, thallus desiccate 90% with biological germ inhibiting effect, saline-alkali tolerant thallus desiccate 0.5%, and herba Typhae velvet powder in balance.
The cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
Example 5
A microbial pesticide for saline-alkali soil capable of being stored for a long time comprises the following components in percentage by weight: 3% of titanium dioxide, 85% of thallus dry matter with the effect of inhibiting biological germs, 2% of saline-alkali resistant thallus dry matter and the balance of cattail wool powder.
The cattail wool powder is a powdery substance obtained by taking down wool on female inflorescences of cattail, soaking and grinding the wool, and performing spray drying and sieving the wool with a 300-mesh sieve.
The dry microbial biomass with the effect of inhibiting biological germs in the microbial pesticides in the saline-alkali soil in the embodiments 1 to 5 is the dry microbial biomass of Brevibacillus laterosporus SNB10, the saline-alkali resistant dry microbial biomass is alkali halophilic aspergillus strain Aspergillus glaucus JL with the preservation number of CGMCC6025, and the number of live spores in the dry microbial biomass of Brevibacillus laterosporus SNB10 and the dry microbial biomass of Aspergillus glaucus JL are both 1 to 2 × 109cfu/mL。
Comparative example 1
The cattail wool powder in the example 1 is removed, and the rest proportion is unchanged.
Comparative example 2
The titanium dioxide in example 1 was removed and the remaining proportion was unchanged.
1. Test material
Medicament: 1g of the microbial pesticide for saline-alkali soil in the embodiments 1-5 and the comparative examples 1-2 (the above pesticides are all microbial pesticides which are stored for 36 months at 10-25 ℃), 1L of water is added to obtain the pesticide, the contrast medicament is 1g of antiviral pesticide ningnanmycin, and clear water spraying is used as a blank contrast.
Pathogen: pseudomonas syringae (Pseudomonas syringae)
Host plant: wheat (Triticum aestivum L.)
2. Test method
The area of the test area was 26.6 square meters, 300 holes per cell, four replicates. Spraying the first time at the seedling stage (sporadic incidence) of wheat for 2 times at intervals of 5-7 days, wherein the spraying amount of the pesticide is 40L/mu.
3. Investigation method
All plants in each cell are investigated, and the investigation morbidity grading standard is divided into 7 grades:
level 0: the whole plant is disease-free;
level 1: the plant is not obviously dwarfed and is shorter than a healthy plant by less than 20 percent;
and 3, level: dwarfing the plant, wherein the height of the plant is 20-35% shorter than that of a healthy plant;
and 5, stage: the plant is seriously dwarfed, and the height of the plant is 35 to 50 percent shorter than that of a healthy plant;
and 7, stage: the plant is severely dwarfed, and the height of the plant is shorter than that of a healthy plant by more than 50 percent or the plant dies.
4. Test results
The disease index and control effect of each treatment are as follows:
5. and (4) conclusion:
after the microbial pesticide for saline-alkali soil is placed for 36 months, the prevention and treatment effect on pseudomonas syringae is still very good.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A microbial pesticide for saline-alkali soil capable of being stored for a long time is characterized by comprising the following components: titanium dioxide, thalli dry matter with the effect of inhibiting biological germs, salt and alkali resistant thalli dry matter and cattail wool powder.
2. The microbial pesticide for saline-alkali soil capable of being stored for a long time according to claim 1, which is characterized by comprising the following components in percentage by weight: 3-8% of titanium dioxide, 85-90% of thallus dry matter with the effect of inhibiting biological germs, 0.5-2% of saline-alkali resistant thallus dry matter and the balance of cattail wool powder.
3. The microbial pesticide for saline-alkali soil capable of being stored for a long time as claimed in claim 1 or 2, wherein the cattail wool powder is a powdery substance obtained by taking down wool on female inflorescence of cattail, then soaking and grinding the removed wool, and performing spray drying and sieving the soaked wool with a 300-mesh sieve.
4. The microbial pesticide for saline-alkali soil capable of being preserved for a long time according to claim 1 or 2, wherein the bacterial cells having biological pathogen inhibitory effect are bacterial strains having biological pathogen inhibitory effect or mutant strains of bacterial strains having biological pathogen inhibitory effect.
5. The microbial pesticide for alkaline land capable of being stored for a long period of time according to claim 1 or 2, wherein the bacterial desiccate having the effect of inhibiting biological germs is a bacterial desiccate of Brevibacillus laterosporus.
6. The microbial pesticide for saline-alkali soil capable of being preserved for a long time according to claim 5, wherein the brevibacillus laterosporus is the brevibacillus laterosporus SNB10, and the strain is preserved in China general microbiological culture Collection center (CGMCC) on 5-17 th month in 2017 with the preservation number: 14171.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010701731.4A CN111670915A (en) | 2020-07-18 | 2020-07-18 | Microbial pesticide capable of being stored for long time in saline-alkali soil |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010701731.4A CN111670915A (en) | 2020-07-18 | 2020-07-18 | Microbial pesticide capable of being stored for long time in saline-alkali soil |
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| CN111670915A true CN111670915A (en) | 2020-09-18 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468524A (en) * | 2002-09-30 | 2004-01-21 | 江苏省农业科学院 | Recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice |
| CN103436450A (en) * | 2013-05-06 | 2013-12-11 | 吉林大学 | Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| RU2701502C1 (en) * | 2018-09-28 | 2019-09-26 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) | Crystal-forming bacterial strain brevibacillus laterosporus with a wide spectrum of antagonist activity and use thereof |
-
2020
- 2020-07-18 CN CN202010701731.4A patent/CN111670915A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1468524A (en) * | 2002-09-30 | 2004-01-21 | 江苏省农业科学院 | Recompounded microbe germicide for preventing and controlling banded sclerotial blight and green smut of rice |
| CN103436450A (en) * | 2013-05-06 | 2013-12-11 | 吉林大学 | Alkaline-tolerant and halophilic aspergillus strain and application thereof in environmental management |
| CN107151641A (en) * | 2017-07-04 | 2017-09-12 | 沈阳农业大学 | One plant suppression Rhizoctonia solani Kuhn Brevibacillus laterosporus and its application |
| RU2701502C1 (en) * | 2018-09-28 | 2019-09-26 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) | Crystal-forming bacterial strain brevibacillus laterosporus with a wide spectrum of antagonist activity and use thereof |
Non-Patent Citations (3)
| Title |
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| 李毅等: "《生物农药》", 30 April 2017, 华中师范大学出版社 * |
| 王杏等: "《纳米二氧化钛的生产与应用》", 31 July 2014, 贵州科技出版社 * |
| 高福军等: "《小学生十万个为什么》", 31 January 2007, 连环画出版社 * |
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Application publication date: 20200918 |