CN111650164A - Microorganism concentration detection system - Google Patents
Microorganism concentration detection system Download PDFInfo
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- CN111650164A CN111650164A CN201910692907.1A CN201910692907A CN111650164A CN 111650164 A CN111650164 A CN 111650164A CN 201910692907 A CN201910692907 A CN 201910692907A CN 111650164 A CN111650164 A CN 111650164A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6467—Axial flow and illumination
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
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Abstract
The invention relates to the field of microorganism detection, in particular to a microorganism concentration detection system. Including treater, high efficiency filter, microorganism mass concentration acquisition mechanism, flowmeter and pump, high efficiency filter passes through three solenoid valve and is connected with the air inlet of microorganism mass concentration acquisition mechanism, the gas that flows from the gas outlet of microorganism mass concentration acquisition mechanism, partly directly flows out through the pipeline, be equipped with flowmeter I and pump I on this pipeline, another part directly flows in the air inlet of microorganism mass concentration acquisition mechanism, be equipped with flowmeter II and pump II on the pipeline of being connected with the gas outlet of microorganism mass concentration acquisition mechanism, flowmeter and pump all are connected with the treater electricity. The device has the advantages of simple structure, light weight, convenience in moving and arrangement, stability, reliability, high sensitivity and high accuracy of the finally obtained detection result.
Description
Technical Field
The invention relates to the field of microorganism detection, in particular to a microorganism concentration detection system.
Background
The microorganisms in the gas have great threat to human health and environmental safety, and researches show that at least more than 100 pathogenic microorganisms in the world can be transmitted through the air, such as tubercle bacillus, influenza virus and the like. The pathogenic microorganisms infect people through respiratory tract, and some types of microorganisms can still infect people and even kill people under the condition of extremely low quantity; the excessive air microorganism content in hospital wards, operating rooms, square cabin type workplaces, ships, submarines and other places has important influence on the health of workers, and diseases are easy to cause and spread. In recent years, outbreaks of public health incidents such as SARS epidemic and H7N9 outbreak have been caused by airborne transmission of pathogenic microorganisms.
At present, the detection process of microorganisms in gas mainly adopts a traditional method of sampling and separation culture, namely, an air sample is firstly collected by a sampler, then a filter membrane is eluted, the microorganisms are cultured, and finally laboratory detection and identification are carried out. Therefore, the technical method for realizing the non-contact detection and the online alarm of the atmospheric microorganisms has great significance to public safety, health and epidemic prevention, environmental monitoring and the like.
The parameter for measuring the concentration of the gas microorganisms by the ultraviolet-visible spectrophotometry is absorbance, the value is related to the ratio of incident light intensity to transmitted light intensity, the incident intensity is increased, the transmitted light intensity is increased, the amplification factor of a detector is increased, and the detection of the incident light and the transmitted light is influenced, so that the improvement of the sensitivity is limited. In addition, the ultraviolet-visible absorption spectrum analysis method is characterized in that light and external valence electrons of substance molecules act, belongs to absorption analysis (many substances can absorb light), and is easy to influence a test result by various factors, so that the test result is inaccurate.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a microorganism concentration detection system which is simple in structure, light in weight, convenient to move and arrange, stable and reliable, high in sensitivity and high in accuracy of a finally obtained detection result.
The technical scheme of the invention is as follows: a microbial concentration detection system comprises a processor, a high-efficiency filter, a microbial mass concentration acquisition mechanism, a flowmeter and a pump, wherein the high-efficiency filter is connected with an air inlet of the microbial mass concentration acquisition mechanism through a three-way electromagnetic valve, one part of gas flowing out of an air outlet of the microbial mass concentration acquisition mechanism directly flows out through a pipeline, the pipeline is provided with the flowmeter I and the pump I, the other part of gas directly flows into the air inlet of the microbial mass concentration acquisition mechanism, the pipeline connected with the air outlet of the microbial mass concentration acquisition mechanism is provided with the flowmeter II and the pump II, and the microbial mass concentration acquisition mechanism, the flowmeter and the pump are all electrically connected with the processor;
the microorganism mass concentration acquisition mechanism comprises an external shell, and an ultraviolet LED light source, a convex lens and a convergent lens which are sequentially arranged in the shell from left to right, wherein the convergent lens is connected with an external spectrometer through an optical fiber, the ultraviolet LED light source is arranged on one side of the shell, the convergent lens is correspondingly arranged on the other side of the shell, light-shielding sheets are arranged in the middle parts of two side surfaces of the convex lens, and an air inlet and an air outlet are arranged on the shell between the convex lens and the convergent lens;
microorganism number concentration acquisition mechanism includes the casing, and set up the ultraviolet laser in the casing, the optic fibre trap, concave lens, light filter and photoelectric detector, ultraviolet laser sets up an inside wall at the casing, be equipped with the optic fibre trap on another inside wall that corresponds with this lateral wall, the middle part of casing is equipped with the Laval spray tube, the top of casing is equipped with the air inlet, the bottom of casing is equipped with the gas outlet, gas passes through in air inlet and the Laval spray tube gets into the casing, be fixed with concave lens on the adjacent inside wall of lateral wall that sets up ultraviolet laser, be equipped with photoelectric detector on the lateral wall corresponding with concave lens, be equipped with the light filter between concave lens and the photoelectric detector, concave lens, light filter and photoelectric detector homogeneous phase are 45 settings for ultraviolet laser beam and Laval spray tube.
In the invention, the channel in the shell between the convex lens and the converging lens comprises two sections, wherein the section close to the convex lens is rectangular, the channel is provided with an air inlet, the section close to the converging lens is horn-shaped, and the channel is provided with an air outlet.
The invention has the beneficial effects that: the system is simple in structure, convenient to use, convenient to move and arrange, stable, reliable, high in sensitivity and high in accuracy of finally obtained data results.
Drawings
FIG. 1 is a schematic diagram of the present system;
FIG. 2 is a schematic view of the structure of a mechanism for collecting the mass concentration of microorganisms;
FIG. 3 is a schematic structural view of a microorganism number concentration acquisition mechanism;
fig. 4 is a sectional view a-a in fig. 3.
In the figure: 1, a high-efficiency filter; 2, a three-way electromagnetic valve; 3 a microorganism mass concentration acquisition mechanism; 4, a flow meter I; 5, pumping a pump I; 6, a pump II; 7; a flowmeter II; 8 a microorganism quantity and concentration acquisition mechanism; 9 an ultraviolet LED light source; 10 light shielding sheets; 11 a convex lens; 12 a converging lens; 13 an optical fiber; 14, a spectrometer; 15 ultraviolet laser; 16 fiber traps; 17 a concave lens; 18 optical filter; 19 photo detector.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below.
In the following description, specific details are set forth in order to provide a thorough understanding of the present invention. The invention can be implemented in a number of ways different from those described herein and similar generalizations can be made by those skilled in the art without departing from the spirit of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
The invention comprises a processor, a high-efficiency filter 1, a microorganism mass concentration acquisition mechanism 4, a microorganism quantity concentration acquisition mechanism 8, a flowmeter and a pump. The high-efficiency filter 1 is connected with an air inlet of a microorganism mass concentration acquisition mechanism 4 through a three-way electromagnetic valve 2. A part of gas flowing out of the gas outlet of the microorganism mass concentration acquisition mechanism 4 directly flows out through a pipeline, and a flowmeter I4 and a pump I5 are arranged on the pipeline; the other part of the microorganism directly flows into an air inlet of the microorganism quantity and concentration acquisition mechanism 8, and a flow meter II 7 and a pump II 6 are arranged on a pipeline connected with an air outlet of the microorganism quantity and concentration acquisition mechanism 8. The pump I5 and the pump II 6 act to enable gas to enter the microorganism mass concentration acquisition mechanism 4 at a certain flow rate, and accurately control the flow of the gas through the flowmeter I4 and the flowmeter II 7 and control the stability of the gas flow. In this embodiment, the gas enters the gas inlet of the microorganism mass concentration acquisition mechanism 4 at a flow rate of 28.3L/min, the gas enters the gas inlet of the microorganism number concentration acquisition mechanism 8 at a flow rate of 1L/min, and the gas flowing through the microorganism number concentration acquisition mechanism 8 and the microorganism mass concentration acquisition mechanism 4 is sampled at a constant speed by controlling the size of the cross section of the gas path. The microorganism mass concentration acquisition mechanism 4, the microorganism number concentration acquisition mechanism 8, the flowmeter and the pump are all electrically connected with the processor.
As shown in fig. 2, the mechanism 4 for collecting the microbial mass concentration includes an external housing, and an ultraviolet LED light source 9, a convex lens 11, and a collecting lens 12 sequentially arranged from left to right in the housing, wherein the collecting lens 12 is connected with an external spectrometer 14 through an optical fiber 13, the ultraviolet LED light source 9 is arranged on one side of the housing, and the collecting lens 12 is correspondingly arranged on the other side of the housing. The middle parts of the two side surfaces of the convex lens 11 are respectively provided with a light shielding sheet 10, the channel in the shell between the convex lens 11 and the convergent lens 12 comprises two sections, one section of the channel close to the convex lens 11 is rectangular, the channel is provided with an air inlet, one section of the channel close to the convergent lens 12 is horn-shaped, and the channel is provided with an air outlet.
In the process of gas flowing, continuous light emitted by the ultraviolet LED light source 9 is shielded by the light shielding sheet 10 arranged on the convex lens 11 to form a regular dark area. After the gas enters a regular dark area, the ultraviolet LED light source 9 is controlled to generate signals of different wave bands by controlling signals of different wave bands, nucleotide, riboflavin, chlorophyll and the like in microorganisms in the gas are induced to generate fluorescent signals of different intensities, the fluorescent signals are transmitted to the optical fiber 13 through the convergent lens 12, and the spectrometer 14 converts the fluorescent signals into electric signals after obtaining the fluorescent signals through the optical fiber 13. The processor collects, processes, analyzes and feeds back the electric signal data, thereby obtaining the mass concentration data of the microorganism.
As shown in fig. 3, the microorganism number concentration acquisition mechanism 8 includes a housing, and an ultraviolet laser 15, an optical fiber trap 16, a concave lens 17, an optical filter 18 and a photodetector 19 which are arranged in the housing, wherein the ultraviolet laser 15 is arranged on one inner side wall of the housing, the optical fiber trap 16 is arranged on the other inner side wall corresponding to the side wall, and a large number of optical fibers are arranged in the optical fiber trap 16. The middle part of casing is equipped with the Laval nozzle, and the top of casing is equipped with the air inlet, and the bottom of casing is equipped with the gas outlet, and gas passes through air inlet and Laval nozzle and gets into in the casing to the gas outlet along the casing bottom flows. A concave lens 17 is fixed on the inner side wall adjacent to the side wall provided with the ultraviolet laser 15, a photoelectric detector 19 is arranged on the side wall corresponding to the concave lens, an optical filter 18 is arranged between the concave lens 17 and the photoelectric detector 19, and the concave lens 17, the optical filter 18 and the photoelectric detector 19 are all arranged at an angle of 45 degrees relative to the ultraviolet laser beam and the Laval nozzle. The light of different wave bands emitted by the ultraviolet laser 15 induces the nucleotide, riboflavin, chlorophyll and the like in the microorganism to generate fluorescence signals with different intensities, the fluorescence signals are transmitted to the optical filter 18 through the optical fiber trap 16 and reach the photoelectric detector 19 through the optical filter 18, the photoelectric detector 19 converts the detected fluorescence signals into electric signals, and the processor collects the electric signals, processes, analyzes and feeds back the electric signal data to obtain the quantity and concentration data of the microorganism.
The microorganism concentration detection system provided by the invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention. The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (2)
1. A system for detecting the concentration of microorganisms, comprising: comprises a processor, a high-efficiency filter (1), a microorganism mass concentration acquisition mechanism (4), a microorganism number concentration acquisition mechanism (8), a flowmeter and a pump, the high-efficiency filter (1) is connected with the air inlet of the microorganism mass concentration acquisition mechanism (4) through a three-way electromagnetic valve (2), one part of the gas flowing out of the air outlet of the microorganism mass concentration acquisition mechanism (4) directly flows out through a pipeline, the pipeline is provided with a flowmeter I (4) and a pump I (5), the other part of the pipeline directly flows into an air inlet of a microorganism quantity and concentration acquisition mechanism (8), a flow meter II (7) and a pump II (6) are arranged on a pipeline connected with an air outlet of the microorganism quantity concentration acquisition mechanism (8), and the microorganism mass concentration acquisition mechanism (4), the microorganism quantity concentration acquisition mechanism (8), the flow meter and the pump are all electrically connected with the processor;
the microorganism mass concentration acquisition mechanism (4) comprises an external shell, and an ultraviolet LED light source (9), a convex lens (11) and a convergent lens (12) which are sequentially arranged in the shell from left to right, wherein the convergent lens (12) is connected with an external spectrometer (14) through an optical fiber (13), the ultraviolet LED light source (9) is arranged on one side of the shell, the convergent lens (12) is correspondingly arranged on the other side of the shell, light shielding sheets (10) are arranged in the middle of two side faces of the convex lens (11), and an air inlet and an air outlet are formed in the shell between the convex lens (11) and the convergent lens (12);
the microorganism number concentration acquisition mechanism (8) comprises a shell, and an ultraviolet laser (15), an optical fiber trap (16), a concave lens (17), an optical filter (18) and a photoelectric detector (19) which are arranged in the shell, wherein the ultraviolet laser (15) is arranged on one inner side wall of the shell, an optical fiber trap (16) is arranged on the other inner side wall corresponding to the side wall, a Laval nozzle is arranged in the middle of the shell, an air inlet is arranged at the top of the shell, an air outlet is arranged at the bottom of the shell, air enters the shell through the air inlet and the Laval nozzle, a concave lens (17) is fixed on the inner side wall adjacent to the side wall provided with the ultraviolet laser (15), the photoelectric detector (19) is arranged on the side wall corresponding to the concave lens, the optical filter (18) is arranged between the concave lens (17) and the photoelectric detector (19), and the concave lens (17), the optical filter (18) and the photoelectric detector (19) are arranged at an angle of 45 degrees relative to the ultraviolet laser beam and the Laval nozzle.
2. The microorganism concentration detection system according to claim 1, characterized in that: the channel in the shell between the convex lens (11) and the convergent lens (12) comprises two sections, one section of the channel close to the convex lens (11) is rectangular, an air inlet is formed in the channel, one section of the channel close to the convergent lens (12) is horn-shaped, and an air outlet is formed in the channel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910692907.1A CN111650164A (en) | 2019-07-30 | 2019-07-30 | Microorganism concentration detection system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910692907.1A CN111650164A (en) | 2019-07-30 | 2019-07-30 | Microorganism concentration detection system |
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| CN111650164A true CN111650164A (en) | 2020-09-11 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2603567A (en) * | 2021-02-03 | 2022-08-10 | D Silver Joshua | Viral load tester and applications thereof |
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2019
- 2019-07-30 CN CN201910692907.1A patent/CN111650164A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2603567A (en) * | 2021-02-03 | 2022-08-10 | D Silver Joshua | Viral load tester and applications thereof |
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Address after: 266000 No. 1 Xueyue Road, Chengyang District, Qingdao City, Shandong Province Applicant after: Qingdao Zhongrui Intelligent Instrument Co.,Ltd. Address before: 266000 No. 1 Xueyue Road, Chengyang District, Qingdao City, Shandong Province Applicant before: QINGDAO ZHONGRUI INTELLIGENT INSTRUMENT Co.,Ltd. |
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Effective date of registration: 20211222 Address after: 266000 No. 10 Jianning Road, Chengyang District, Qingdao City, Shandong Province Applicant after: Qingdao Zhongrui Intelligent Instrument Co.,Ltd. Applicant after: Academy of military medicine, PLA Academy of Military Sciences Address before: 266000 No. 1 Xueyue Road, Chengyang District, Qingdao City, Shandong Province Applicant before: Qingdao Zhongrui Intelligent Instrument Co.,Ltd. |