KR20180096203A - MIRRIRLESS OPTICAL DETECTION APPARATUS of MICROORGANISM - Google Patents

Abstract

The present invention relates to an optical type microorganism detecting apparatus, comprising: a lens barrel including a nozzle portion through which a sample to be measured is introduced, a light incident port to which incident light is incident, and a light exit port for injecting the incident light irradiated on the sample to be measured; a light sending portion realized as a singular LED, irradiating the incident light on the light incident port, and blocking surrounding light introduced to the incident light; and a light collecting portion for separating the exit light injected from the light exit port to transfer to a first path and a second path, transferring the exit light having a same wavelength with that of the incident light to the first path, and blocking the surrounding light introduced to the exit light transferred to the second path to detect fluorescence, thereby capable of minimizing production costs.

Description

TECHNICAL FIELD [0001] The present invention relates to an optical microbial detection device having no reflector,

The present invention relates to an optical microbe detection apparatus without a reflector, and more particularly, to an optical microbe detection apparatus which is simple in structure without a reflector and is small in size and low in production cost.

As the industry develops, the generation of pollutants is also increasing. These pollutants contain various harmful substances such as fine dusts and microorganisms. In recent years, it has been found that among such pollutants, materials such as fine dusts and microorganisms that can be frequently encountered in the environment can have a fatal effect on the human body. At the national level, it is also possible to predict the dustiness, . In addition to forecasting national or regional scales to prevent and minimize substantial damage to micro dust and microorganisms, continuous monitoring and countermeasures in public spaces or facilities where people are inundated are essential. In accordance with these demands, research and development on devices capable of highly precise detection of fine dusts and microorganisms are being continuously carried out. Conventional microorganism detection techniques have been used to collect samples for measurement in the atmosphere, to culture the collected samples in a medium, and to detect microorganisms through the number of cultured microorganisms and the like. However, this method has a disadvantage in that it takes several hours to several days or more to cultivate the captured microorganisms. Recently, researches on an optical detector capable of monitoring the atmospheric state in real time have been actively conducted.

1 is a view showing a conventional optical detector for detecting fine dust and microorganisms. The optical detector shown in Fig. 1 irradiates the measurement sample in the sample chamber 31 with light while the measurement sample aerosol flows into the sample chamber 31 whose inner wall surface is the ellipsoidal mirror 32 , And collects scattered light and fluorescence generated by collision with the measurement sample to detect fine dust and microorganisms. However, since the conventional optical detector has an expensive sample chamber whose inner wall surface is made of the ellipsoidal mirror 32, the production cost is increased, and the volume occupied by the sample chamber in the optical detector can not be reduced. there was. Further, since the sample chamber must be unconditionally provided, there is a problem that it is difficult to simplify the structure of the optical detector.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an optical microbe detection apparatus that does not have a reflector capable of minimizing a production cost by removing a sample chamber having an inner wall surface as a reflector in an optical microbe detection process.

Another object of the present invention is to provide an optical microorganism detecting apparatus which does not have a reflector capable of being downsized by removing a sample chamber having an inner wall surface made of a reflecting mirror.

Another technical problem to be solved by the present invention is to provide an optical microorganism detecting apparatus which does not have a simple reflector by removing a sample chamber whose inner wall surface is a reflector.

The optical microbe detection apparatus without a reflector according to an embodiment of the present invention includes a nozzle unit into which a measurement sample is introduced, a light incidence port through which incident light is incident, and a light output port through which the incident light is irradiated onto the measurement sample Collecting barrel; A light emitting unit which is implemented as a single LED and emits the incident light to the light incidence unit and blocks ambient light introduced into the incident light; And a second path for transmitting the emitted light having the same wavelength as that of the incident light to the first path and transmitting the outgoing light having the same wavelength as the incident light to the first path and the second path, And a light receiving unit for detecting fluorescence by blocking ambient light.

The light-emitting unit may further include a first light-transmitting lens for converting the light irradiated by the LED into parallel light, and a second light-transmitting lens for collecting the parallel light generated by the first light-transmitting lens into the nozzle unit of the light- And the blocking member may include a plurality of light control units for blocking the ambient light incident on the incident light.

The light receiving portion may include a first condenser lens portion for condensing the outgoing light emitted from the light output port; A spectroscopic element that reflects or transmits outgoing light condensed by the first condenser lens; And a fluorescence detector for detecting fluorescence from the outgoing light transmitted through the spectroscopic element.

In addition, the first condenser lens portion can collect 70% or more of the outgoing light emitted from the light output port.

The fluorescence detection unit may include a fluorescence filter unit that passes only the outgoing light of a specific wavelength;

A second condenser lens unit for condensing emitted light passed through the fluorescent filter unit; And a light adjusting unit for removing the light noise included in the outgoing light condensed by the second condensing lens unit.

Further, the light control unit may include a plurality of light control units, and each of the plurality of light control units may have an opening formed at a central portion thereof.

Further, the light control units may be arranged so that a smaller aperture is formed closer to the condensing focus of the second condenser lens part.

According to another embodiment of the present invention, there is provided an optical microbe detection apparatus without a reflector, including a nozzle unit into which a measurement sample is introduced, a light incidence port through which incident light is incident, and an optical exit port through which the incident light is irradiated onto the measurement sample Collecting barrel; A light emitting unit which is implemented as a single LED and emits the incident light to the light incidence unit and blocks ambient light introduced into the incident light; And a second path for transmitting the emitted light having the same wavelength as that of the incident light to the first path and for emitting the light having a wavelength different from that of the incident light to the second path, And transmits the emitted light to the third path and the fourth path, and cuts off ambient light introduced into the outgoing light of a wavelength different from that of the incident light transmitted to the third path, And a light receiving unit for detecting fluorescence.

The light receiving portion may include a first condenser lens portion for condensing the outgoing light emitted from the light output port; A first spectroscopic element that reflects outgoing light having the same wavelength as the incident light out of the outgoing light focused by the first condensing lens and passes the outgoing light of a different wavelength from the outgoing light; A second spectroscopic element for reflecting the outgoing light of a wavelength different from the incident light out of the outgoing light passed through by the first spectroscopic element and passing the outgoing light of the same wavelength as the incident light out of the outgoing light; And a fluorescence detector for detecting fluorescence in the outgoing light reflected by the second spectroscopic element.

In addition, the first condenser lens portion can collect 70% or more of the outgoing light emitted from the light output port.

The fluorescence detection unit may include a fluorescence filter unit that passes only the outgoing light of a specific wavelength; A second condenser lens unit for condensing emitted light passed through the fluorescent filter unit; And a light adjusting unit for removing the light noise included in the outgoing light condensed by the second condensing lens unit.

Further, the light control unit may include a plurality of light control units, and each of the plurality of light control units may have an opening at a central portion thereof.

Further, the light control units may be arranged so that a smaller aperture is formed closer to the condensing focus of the second condenser lens part.

As described above, the optical microbe detection apparatus without a reflector according to an embodiment of the present invention has the effect of minimizing the production cost of the optical microbe detection apparatus by removing the sample chamber having the inner wall surface of the reflector.

In addition, the optical microbe detection apparatus without a reflector according to an embodiment of the present invention can reduce the size of the optical microbe detection apparatus by removing a sample chamber having an inner wall surface formed of a reflector.

In addition, the optical microbe detection apparatus without a reflector according to an embodiment of the present invention has an effect of simplifying the structure of the optical microbe detection apparatus by removing a sample chamber having an inner wall surface formed of a reflector.

In addition, the optical microbe detection apparatus without a reflector according to an embodiment of the present invention can significantly improve the microbial detection performance by differently detecting the fluorescence from the emitted light according to the light noise included in the incident light .

In addition, the optical microbe detection device without a reflector according to an embodiment of the present invention can simplify the structure of the light emitting part using only LEDs, reduce the production cost of the optical microbe detection device, and miniaturize the optical microbe detection device have.

In addition, the optical microbe detection apparatus without a reflector according to an embodiment of the present invention includes a plurality of spectroscopic elements, thereby minimizing the influence of diffuse reflection.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS In order to more fully understand the drawings recited in the detailed description of the present invention, a detailed description of each drawing is provided.
1 is a view showing a conventional optical detector for detecting fine dust and microorganisms.
2 is a view showing an optical microbe detection apparatus without a reflector according to the present invention.
3 is a diagram showing the degree (a) of the fluorescence passing through the band-pass filter and the figure (b) in which light other than fluorescence is blocked by the band-pass filter.
4 is a diagram illustrating a light receiving unit according to an embodiment of the present invention in more detail.
FIG. 5 is a view showing another embodiment of the light-transmitting portion of the optical microbe detection device without a reflector according to the present invention shown in FIG. 2. FIG.
6 is a view showing an optical microbe detection apparatus without a reflector according to another embodiment of the present invention.
7 is a view showing a light receiving unit according to another embodiment of the present invention in more detail.

It is to be understood that the specific structural or functional descriptions of embodiments of the present invention disclosed herein are only for the purpose of illustrating embodiments of the inventive concept, But may be embodied in many different forms and is not limited to the embodiments set forth herein.

Embodiments in accordance with the concepts of the present invention are capable of various modifications and may take various forms, so that the embodiments are illustrated in the drawings and described in detail herein. It should be understood, however, that it is not intended to limit the embodiments according to the concepts of the present invention to the particular forms disclosed, but includes all modifications, equivalents, or alternatives falling within the spirit and scope of the invention.

The terms first, second, etc. may be used to describe various elements, but the elements should not be limited by the terms. The terms are intended to distinguish one element from another, for example, without departing from the scope of the invention in accordance with the concepts of the present invention, the first element may be termed the second element, The second component may also be referred to as a first component.

It is to be understood that when an element is referred to as being "connected" or "connected" to another element, it may be directly connected or connected to the other element, . On the other hand, when an element is referred to as being "directly connected" or "directly connected" to another element, it should be understood that there are no other elements in between. Other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise.

In this specification, the terms "comprises" or "having", etc. are intended to specify the presence of stated features, integers, steps, operations, elements, parts or combinations thereof, , Steps, operations, components, parts, or combinations thereof, as a matter of principle.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the meaning of the context in the relevant art and, unless explicitly defined herein, are to be interpreted as ideal or overly formal Do not.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the preferred embodiments of the present invention with reference to the accompanying drawings.

2 is a view showing an optical microbe detection apparatus without a reflector according to the present invention.

2, an optical microorganism detection apparatus (hereinafter, referred to as 'detection apparatus') 10 having no reflector according to an embodiment of the present invention includes a condensing section barrel 100 into which a measurement sample flows, A light emitting unit 200 for emitting light to the light emitting unit 100 and a light receiving unit 300 for detecting fluorescence light emitted from the light emitting unit 140 of the light collecting unit main body 100.

A measurement sample, for example, a sample aerosol, is introduced through the sample inlet 120 of the condenser barrel 100.

An inlet nozzle of a nozzle unit (not shown) is connected to the sample inlet 120 of the body 110 of the condenser main body 100. A sample aerosol to be measured through the inlet nozzle is passed through the condenser main body 100, Respectively.

Although not shown in the drawing, the body 110 of the lens barrel 100 on the opposite side of the sample inlet 120 is provided with a sample outlet to discharge the introduced sample aerosol to the outside of the lens barrel 100.

The light collecting barrel 100 is provided with a light incidence hole 130 so as to correspond to the light transmitting portion 200 described later.

In addition, the light collecting portion lens barrel 100 is provided with a light output port 140 so as to correspond to a light receiving portion 300 described later.

The optical microorganism detection apparatus without a reflector according to an embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

The light emitting unit 200 that emits light to the light collecting unit main body 100 irradiates UV light using an LED (light emitting diode) 210 to a measurement sample in the light collecting unit main body 100 through the light incident unit 130 And the irradiated UV light collides with the aerosol particles flowing into the condenser lens barrel 100 to generate scattering light and fluorescence light.

At this time, the LED 210, which is a light emitting element of the light emitting unit 200, may be arranged to emit light in a direction of incidence to the light collecting unit barrel 100, that is, in the direction of the light incident unit 130, It is possible to emit light in the UV region of 40 to 40 nm. It is possible to emit light in the UV region of preferably 340 nm to 380 nm to minimize fluorescence of a inanimate object.

The light-emitting unit 200 may be implemented with only a single LED 210, and the reason for this is that Mie scattering.

Mie scattering refers to the scattering direction of the incoming light determined by the relationship between the wavelength of the incident light and the size of the particles in the air.

When the particle size is larger than the wavelength of the incident light by a factor of several tens of times and is smaller than several hundreds of times, most of the incident light scattering by colliding with the particles is directed forward by Mie scattering.

Therefore, when Mie scattering can be applied, when the particle size is larger than the wavelength of the incident light by a factor of several tens times, and is smaller than several hundreds of times, most of the incident light that is scattered by the particles collides with the incident light It is possible to detect fluorescence that is incident light that is scattered when a light receiving unit 300, which will be described later, is provided in the direction in which the incident light is incident.

That is, when Mie scattering is applied even when the light-emitting unit 200 is implemented by a single LED 210, the optical microbe detection apparatus may include a light-collecting chamber having an inner wall surface formed by a mirror, It is possible for the light receiving unit 300 of the optical microorganism detection apparatus to detect the fluorescence without condensing the fluorescence at the preset point.

The optical microorganism detection apparatus without a reflector according to an embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

In addition, since the light emitting unit 200 is implemented by only the single LED 210, the production cost can be minimized as compared with the existing microbial detection device, and the possibility of miniaturization is much higher than the existing microbial detection device. The structure of the optical microorganism detection apparatus is much simpler than that of the microorganism detection apparatus of the present invention.

2, the light receiving unit 300 for detecting fluorescence light emitted from the light output port 140 of the light collecting unit main body 100 includes a first condensing lens unit 310, a spectroscopic element 320, And a fluorescence detection unit 330.

The first condensing lens unit 310 functions to convert light (for example, fluorescence) emitted from the light output port 140 of the condenser portion mirror tube 100 into a parallel beam.

The first condensing lens unit 310 may be implemented as a collimator lens. In this case, a collimator lens having a relatively large NA (Numerical Aperture) value is used.

For example, when the NA (Numerical Aperture) value of the collimator lens is 0.6, the incident angle is a positive minus 36.8 degrees. When the angle of incidence is positive 36.8 degrees, it is possible to collect 70% or more of the fluorescent light scattered in the light collecting part tube 100. [

The spectroscopic element 320 reflects the scattered light whose wavelength is not changed among the light passing through the first condenser lens part 310 to the first path and allows the fluorescence whose wavelength is changed to pass through the second path.

FIG. 2 is a view showing an optical microbe detection apparatus without a reflector according to the present invention. FIG. 3 is a graph showing the degree (a) of passage of fluorescence through a bandpass filter and that of light other than fluorescence FIG. 4 is a view illustrating a light receiving unit according to an embodiment of the present invention in more detail. Referring to FIG.

2, the fluorescence detector 330 includes a fluorescence inlet 331, a fluorescence filter 340, a second condenser lens 350, a light controller 360, and a fluorescence sensor 370 .

 The fluorescent inlet 331 is an aperture formed to pass scattered light that has passed through the spectroscopic element 320 in the second path.

The fluorescence filter unit 340 filters the scattered light passing through the fluorescence inlet 331.

The band pass filter of the fluorescence filter unit 340 has a value greater than an optical density 6 in order to block light other than the fluorescence wavelength.

Here, the optical density of the band-pass filter refers to the ability to block light of a specific wavelength.

3 (a), when the optical density of the band-pass filter is greater than 6, 95% or more ((1)) of the fluorescence (wavelength of 480 nm) And passes through the filter unit 340.

3 (b), when the optical density of the band-pass filter is greater than 6, the lights other than fluorescence emitted to the fluorescent filter unit 340 are mostly blocked by the fluorescent filter unit 340 blocking ((2)).

Referring to FIG. 4, the second condenser lens unit 350 converges the parallel scattered light passing through the fluorescent filter unit 340 to a fluorescence sensor unit 370 described later.

 Referring to FIG. 4, the light control unit 360 may include a plurality of light control units. In FIG. 4, only three light control units are shown for convenience of explanation, but three or more Can be applied.

An aperture for passing incident light transmitted from the second condensing lens unit 350 to the fluorescence sensor unit 370 is formed in the central portion of each of the plurality of light control units, The closer to the focus 361, the smaller is set.

That is, the plurality of light control units have relatively large openings as they are farther to the left and right about the focal point 361.

Therefore, the light control unit 360 can prevent the infiltrating signal that has passed through the fluorescent filter unit 340 from entering the fluorescence sensor unit 370, thereby improving the fluorescence detection ability, that is, the ability to detect microorganisms It is effective.

The fluorescence sensor unit 370 detects the presence and amount of microorganisms from the transmitted light.

That is, the fluorescence sensor unit 370 receives the fluorescence emitted to the outside of the condenser barrel 100, generates a detection signal for the received fluorescence, and transmits the detection signal to a signal processing unit (not shown).

The fluorescence sensor 371 of the fluorescence sensor unit 370 can be implemented as a photomultiplier tube (PMT), and the fluorescence detected by the fluorescence sensor 371 can be realized by a micro- And information on the presence and amount of microorganisms.

The signal detected by the fluorescence sensor unit 370 is transmitted to the signal processing unit to calculate the presence or amount of microorganisms according to a predetermined algorithm.

According to the embodiment, the detection device 10 may further include an optical stopper part 400 on the upper side of the light receiving part 300.

The optical stopper unit 400 condenses the scattered light of which the wavelength is not changed by the spectroscopic element 320 reflected by the first path, by the condenser lens 410, and stops the scattered light.

That is, the optical stopper unit 400 stops the light reflected from the first path by the spectroscopic element 320, thereby minimizing the inflow of ambient light other than fluorescence into the light-receiving unit 300.

The optical stopper part 400 may include a conical member 420 and the conical member 420 may be arranged to face the path of the light from which the vertex is emitted and the case 430 may be wrapped around the conical member 420 . Therefore, it is possible to prevent direct reflection of the light impinging on the conical member 420.

In addition, a member for absorbing light such as a sponge may be disposed on the surface of the conical member 420 and the surface of the case 430 corresponding to the surface of the conical member 420, or may be implemented with a concavo-convex structure.

FIG. 5 is a view showing another embodiment of the light-transmitting portion of the optical microbe detection device without a reflector according to the present invention shown in FIG. 2. FIG.

5 is similar to the optical microbe detection apparatus not having the reflector shown in Fig. 2, only the differences are described in order to avoid duplication of description.

5, the light emitting unit 200 includes an LED 210, a blocking member 220, a first light emitting lens 230, and a second light emitting lens 240.

The LED 210 as a light emitting element of the light emitting unit 200 may be arranged to emit light in the direction of incidence to the light collecting unit column 100, that is, in the direction of the light incident aperture 130, It is possible to emit light in the UV region of 405 nm. It is possible to emit light in the UV region of preferably 340 nm to 380 nm to minimize fluorescence of a inanimate object.

The LED 210 may be a single LED. The use of a single LED as a light source is advantageous not only in reducing the system cost and achieving miniaturization but also in solving the alignment problem caused by using two light sources, rather than using a general laser or a laser diode (LD) This is because there are advantages.

5, the blocking member 220 may be disposed between the LED 210 and the first light-transmitting lens 230 and may include a plurality of light control units a1, a2, a3, a4. have.

5, only four light control units a1, a2, a3, and a4 are illustrated for convenience of explanation, but the present invention is not limited thereto, and four or more light control units may be applied according to the design.

At the center of each of the plurality of light control units a1, a2, a3 and a4, apperture for passing incident light from the LED 210 is formed.

At this time, the plurality of light control units a1, a2, a3 and a4 are arranged in the order of the first unit, the second unit, the third unit and the fourth unit having a relatively large opening as the first light- In order.

Therefore, the blocking member 220 functions to minimize light noise by blocking ambient light incident on the incident light transmitted from the LED 210 to the first light-transmitting lens 230.

The first light-transmitting lens 230 serves to convert the incident light passed through the blocking member 220 into parallel light.

The second light-transmitting lens 240 collects the parallel light generated by the first light-transmitting lens 230 to the nozzle unit 120 of the condenser lens barrel 100.

The optical microorganism detection apparatus without a reflector according to an embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

The light emitted by the LED 210 light source is precisely focused on the nozzle unit 120 of the condenser lens barrel 100 by using the first and second light-transmitting lenses 230 and 240, Can be dramatically improved.

6 is a view showing an optical microbe detection apparatus without a reflector according to another embodiment of the present invention.

Referring to FIG. 6, an optical microorganism detecting apparatus (hereinafter, referred to as 'detecting apparatus') 10 having no reflector according to another embodiment of the present invention includes a condensing section barrel 600 through which a measurement sample flows, A light emitting unit 500 for emitting light to the barrel 600 and a light receiving unit 700 for detecting fluorescence light emitted from the light emitting hole 640 of the light collecting unit barrel 600.

A measurement sample, for example, a sample aerosol, is introduced through the sample inlet 620 of the condenser barrel 600.

An inlet nozzle of a nozzle unit (not shown) is connected to the sample inlet 620 of the body 610 of the condenser unit barrel 600 and a sample aerosol to be measured is introduced through the inlet nozzle into the condenser barrel 600, Respectively.

Although not shown in the drawing, a sample outlet is provided in the body 610 of the condenser barrel 600 opposite to the sample inlet 620 to discharge the introduced sample aerosol to the outside of the condenser portion barrel 600.

The light collecting barrel 600 is provided with a light incidence hole 630 so as to correspond to a light transmitting portion 500 described later.

Further, a light output port 640 is provided in the light collecting portion lens barrel 600 so as to correspond to a light receiving portion 700 described later.

The optical microorganism detection apparatus without a reflector according to another embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

The light emitting unit 500 that emits light to the light collecting unit tube 600 irradiates UV light using an LED (Light Emitting Diode) to the measurement sample in the light collecting unit tube 600 through the light incidence hole 630, The irradiated UV light collides with the aerosol particles flowing into the condenser lens barrel 600 to generate scattering light and fluorescence light.

At this time, the LED 510, which is a light emitting element of the light emitting unit 500, may be arranged to emit light in the direction of incidence to the light collecting unit column 600, that is, in the direction of the light entrance aperture 630, It is possible to emit light in the UV region of 40 to 40 nm. It is possible to emit light in the UV region of preferably 340 nm to 380 nm to minimize fluorescence of a inanimate object.

The light-emitting unit 500 may be implemented with only a single LED 510, which is possible because of Mie scattering.

Mie scattering refers to the scattering direction of the incoming light determined by the relationship between the wavelength of the incident light and the size of the particles in the air.

When the particle size is larger than the wavelength of the incident light by a factor of several tens of times and is smaller than several hundreds of times, most of the incident light scattering by colliding with the particles is directed forward by Mie scattering.

Therefore, when Mie scattering can be applied, when the particle size is larger than the wavelength of the incident light by a factor of several tens times, and is smaller than several hundreds of times, most of the incident light that is scattered by the particles collides with the incident light It is possible to detect fluorescence that is incident light that is scattered when a light receiving unit 700 to be described later is provided in the direction in which the incident light is directed.

That is, when Mie scattering is applied even when the light-emitting unit 500 is implemented by a single LED 510, the optical microbe detection apparatus may include a light-collecting chamber having an inner wall surface formed by a mirror, It is possible for the light receiving unit 700 of the optical microorganism detection apparatus to detect fluorescence without condensing the fluorescence at the preset point.

The optical microorganism detection apparatus without a reflector according to another embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

In addition, since the light emitting unit 500 is implemented by only the single LED 510, the production cost can be minimized as compared with the existing microbial detection apparatus, and the possibility of miniaturization is much higher than the existing microbial detection apparatus. The structure of the optical microorganism detection apparatus is much simpler than that of the microorganism detection apparatus of the present invention.

6, a light receiving unit 700 for detecting fluorescence light emitted from a light output port 640 of the light collecting unit tube 600 includes a first condensing lens unit 710, a first spectral element 720 ), A second spectroscopic element 730 and a fluorescence detector 740.

The first condensing lens unit 710 functions to convert light (for example, fluorescence) emitted from the light output port 640 of the condenser portion mirror tube 600 into a parallel beam.

The first condensing lens unit 710 may be implemented as a collimator lens, and a collimator lens having a relatively large NA (Numerical Aperture) value may be used.

For example, when the NA (Numerical Aperture) value of the collimator lens is 0.6, the incident angle is a positive minus 36.8 degrees. When the angle of incidence is positive 36.8 degrees, it is possible to collect 70% or more of the fluorescent light scattered in the light collecting part tube 100. [

  The first spectroscopic element 720 reflects the scattered light whose wavelength is not changed among the light passing through the first condensing lens part 710 to the first path and the fluorescence whose wavelength is changed to pass through the second path.

The second spectroscopic element 730 reflects, by the third path, the fluorescence that is the outgoing light having a wavelength different from that of the incident light that the first spectroscopic element 720 has passed through the second path, and outputs the same wavelength The light passes through the fourth path.

Since the second spectroscopic element 730 reflects only light having a wavelength corresponding to the fluorescence, the light having a wavelength other than the fluorescence included in the outgoing light passed through the second path by the first spectroscopic element 720 is transmitted through the second spectroscopic element 730, and is stopped.

Since the second spectroscopic element 730 reflects only the light having the wavelength corresponding to the fluorescence to the fluorescence detecting unit 740 to be described later, the influence of the diffuse reflection caused by the characteristics of the incoherent optical source LED Can be minimized.

The optical microorganism detection apparatus without a reflector according to another embodiment of the present invention can minimize the production cost as compared with the conventional microorganism detection apparatus by removing the sample chamber having the inner wall surface made of the reflector, The possibility of miniaturization is much higher than that of conventional microbial detection device, and the structure of the optical microbial detection device is much simpler than the conventional microbial detection device.

In addition, since the microbial detection device has a structure capable of reflecting only fluorescence with a plurality of spectroscopic elements, the possibility of removing light noise due to diffuse reflection is much higher than that of the conventional microbial detection device.

FIG. 3 is a view showing the degree (a) of fluorescence passing through the band-pass filter and the state (b) in which light other than fluorescence is blocked by the band-pass filter, and FIG. FIG. 7 is a view showing a light receiving unit according to another embodiment of the present invention in more detail. FIG.

6, the fluorescence detection unit 740 includes a fluorescence inlet 741, a fluorescence filter unit 750, a second condenser lens unit 760, a light control unit 770, and a fluorescence sensor unit 780 .

 Fluorescent inlet 741 is an aperture formed to pass scattered light that has passed through second spectral element 730 to a third path.

The fluorescence filter unit 750 filters the scattered light passing through the fluorescence inlet 741.

The fluorescent filter unit 750 is a band-pass filter, and the band-pass filter of the fluorescent filter unit 750 has a value greater than an optical density 6 in order to block light other than the fluorescent light.

Here, the optical density of the band-pass filter refers to the ability to block light of a specific wavelength.

3 (a), when the optical density of the band-pass filter is larger than 6, 95% or more ((1)) of the fluorescence (wavelength of 480 nm) And passes through the filter unit 750.

3B, when the optical density of the band-pass filter is greater than 6, the lights other than fluorescence emitted to the fluorescence filter unit 750 are mostly blocked by the fluorescence filter unit 750 blocking ((2)).

7, the second condenser lens unit 760 converges the parallel fluorescence light having passed through the fluorescence filter unit 750 to a fluorescence sensor unit 780 to be described later.

 Referring to FIG. 7, the light control unit 770 may include a plurality of light control units. In FIG. 7, only three light control units are shown for convenience of explanation, but three or more Can be applied.

An aperture for passing incident light transmitted from the second condensing lens unit 760 to the fluorescence sensor unit 780 is formed in the central portion of each of the plurality of light control units, The closer to the focus 771, the smaller is set.

That is, the plurality of light control units have relatively large apertures as they are farther to the left and right about the focal point 771.

Therefore, the light control unit 770 can prevent the infiltrating signal that has passed through the fluorescent filter unit 750 from entering the fluorescence sensor unit 780, thereby improving the fluorescence detection capability, that is, the ability to detect microorganisms It is effective.

The fluorescence sensor unit 780 detects the presence and amount of microorganisms from the transmitted light.

That is, the fluorescence sensor unit 780 receives the fluorescence emitted from the condenser lens barrel 600, generates a detection signal for the received fluorescence, and transmits the detection signal to a signal processing unit (not shown).

The fluorescence sensor 781 of the fluorescence sensor unit 780 can be realized as a photo multiplier tube (PMT), and the fluorescence detected by the fluorescence sensor 781 can be realized by a photo- And information on the presence and amount of microorganisms.

The signal detected by the fluorescence sensor unit 780 is transmitted to the signal processing unit to calculate the presence or amount of microorganisms according to a predetermined algorithm.

According to the embodiment, the detection device 10 is configured such that the first spectroscopic element 720 corresponds to the first path for reflecting the scattered light whose wavelength is not changed among the light passing through the first condenser lens part 710, (800).

The optical stopper unit 800 stops the scattered light whose wavelength is not changed by the first spectroscopic element 720 through the first path through the optical stopper optical incidence aperture 810.

That is, the optical stopper unit 800 serves to stop the light reflected by the first spectroscopic element 720 to the first path, thereby minimizing the introduction of ambient light other than fluorescence into the second spectroscopic element 730 can do.

The optical stopper unit 800 may include a conical member 820 and the conical member 820 may be arranged to face the path of the light from which the vertex is emitted and to surround the case 830 . Therefore, it is possible to prevent direct reflection of light impinging on the conical member 820. [

In addition, a light absorbing member such as a sponge may be disposed on the surface of the conical member 820 and the surface of the case 830 corresponding to the surface of the conical member 820, or may be embodied by a concavo-convex structure.

 As used herein, the term " part " refers to a logical building block, and is not necessarily a physically distinct component. It is obvious to those skilled in the art to which the present invention belongs.

While the present invention has been described in connection with what is presently considered to be the most practical and preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments. That is, within the scope of the present invention, all of the components may be selectively coupled to at least one.

In addition, although all of the components may be implemented as one independent hardware, some or all of the components may be selectively combined to perform a part or all of the functions in one or a plurality of hardware. As shown in FIG. The codes and code segments constituting the computer program may be easily deduced by those skilled in the art.

Such a computer program can be stored in a computer-readable storage medium, readable and executed by a computer, thereby realizing an embodiment of the present invention. As a storage medium of the computer program, a magnetic recording medium, an optical recording medium, or the like can be included.

It is also to be understood that the terms such as " comprises, "" comprising," or "having ", as used herein, mean that a component can be implanted unless specifically stated to the contrary. But should be construed as including other elements.

All terms, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used terms, such as predefined terms, should be interpreted to be consistent with the contextual meanings of the related art, and are not to be construed as ideal or overly formal, unless expressly defined to the contrary.

The foregoing description is merely illustrative of the technical idea of the present invention, and various changes and modifications may be made by those skilled in the art without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are intended to illustrate rather than limit the scope of the present invention, and the scope of the technical idea of the present invention is not limited by these embodiments. The scope of protection of the present invention should be construed according to the following claims, and all technical ideas within the scope of equivalents should be construed as falling within the scope of the present invention.

100 ... concentrator tube 200 ... emitter
300 ... light receiving section 400 ... optical stopper section
500 ... Transmitting part 600 ... Collecting part of lens barrel
700 ... light receiving section 800 ... optical stopper section

Claims (13)

A light collecting portion lens barrel including a nozzle portion into which a measurement sample is introduced, a light incidence portion through which incident light is incident, and a light output port through which the incident light is irradiated onto the measurement sample;
A light emitting unit including a single LED for emitting the incident light to the light incidence unit and a blocking member for blocking ambient light introduced into the incident light; And
A first path and a second path for transmitting the outgoing light emitted from the light output port to the first path and transmitting the outgoing light having the same wavelength as the incident light to the first path, And a light receiving unit for detecting fluorescence by blocking ambient light. The method according to claim 1,
The light-
Further comprising a first light-transmitting lens for converting the light irradiated by the LED into a parallel light, and a second light-transmitting lens for collecting parallel light created by the first light-transmitting lens into a nozzle portion of the light-
Wherein the blocking member comprises a plurality of light control units that block the ambient light that is incident on the incident light. The method according to claim 1,
The light-
A first condenser lens portion for condensing emitted light emitted from the light output port;
A spectroscopic element that reflects or transmits outgoing light condensed by the first condenser lens; And
And a fluorescence detecting unit for detecting fluorescence from the outgoing light transmitted through the spectroscopic element. The method of claim 3,
Wherein the first condenser lens unit does not include a reflector for condensing 70% or more of the outgoing light emitted from the light output port. The method of claim 3,
The fluorescence detector
A fluorescence filter portion for passing only emitted light of a specific wavelength;
A second condenser lens unit for condensing emitted light passed through the fluorescent filter unit; And
And a light control unit for removing the light noise included in the outgoing light condensed by the second condenser lens unit. 6. The method of claim 5,
Wherein the light control unit includes a plurality of light control units and each of the plurality of light control units does not have a reflector in which an opening is formed at a central portion thereof. The method according to claim 6,
Wherein the light control units do not have a reflector in which a smaller aperture is formed and arranged so as to be closer to the condensing focus of the second condenser lens part. A light collecting portion lens barrel including a nozzle portion into which a measurement sample is introduced, a light incidence portion through which incident light is incident, and a light output port through which the incident light is irradiated onto the measurement sample;
A light emitting unit which is implemented as a single LED and emits the incident light to the light incidence unit and blocks ambient light introduced into the incident light; And
Wherein the first path and the second path are formed by a first path and a second path, the first path and the second path are connected to each other through a first path, And transmits the emitted light to the third path by separating the emitted light into the third path and the fourth path and transmits the emitted light to the third path by blocking the ambient light entering the outgoing light of a different wavelength from the incident light transmitted through the third path, And a photodetector for detecting the photocatalytic activity of the photocatalyst. 9. The method of claim 8,
The light-
A first condenser lens portion for condensing emitted light emitted from the light output port;
A first spectroscopic element that reflects outgoing light having the same wavelength as the incident light out of the outgoing light focused by the first condensing lens and passes the outgoing light of a different wavelength from the outgoing light;
A second spectroscopic element for reflecting the outgoing light of a wavelength different from the incident light out of the outgoing light passed through by the first spectroscopic element and passing the outgoing light of the same wavelength as the incident light out of the outgoing light; And
And a fluorescence detecting unit for detecting fluorescence from the outgoing light reflected by the second spectroscopic element. 10. The method of claim 9,
Wherein the first condenser lens unit does not include a reflector for condensing 70% or more of the outgoing light emitted from the light output port. 10. The method of claim 9,
The fluorescence detector
A fluorescence filter portion for passing only emitted light of a specific wavelength;
A second condenser lens unit for condensing emitted light passed through the fluorescent filter unit; And
And a light control unit for removing the light noise included in the outgoing light condensed by the second condenser lens unit. 12. The method of claim 11,
Wherein the light control unit includes a plurality of light control units and each of the plurality of light control units does not have a reflector in which an opening is formed at a central portion thereof. 13. The method of claim 12,
Wherein the light control units do not have a reflector in which a smaller aperture is formed and arranged so as to be closer to the condensing focus of the second condenser lens part.

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