CN111650147A - Method for testing irritation of surfactant - Google Patents
Method for testing irritation of surfactant Download PDFInfo
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- CN111650147A CN111650147A CN202010447191.1A CN202010447191A CN111650147A CN 111650147 A CN111650147 A CN 111650147A CN 202010447191 A CN202010447191 A CN 202010447191A CN 111650147 A CN111650147 A CN 111650147A
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- 239000004094 surface-active agent Substances 0.000 title claims abstract description 64
- 238000012360 testing method Methods 0.000 title claims abstract description 54
- 230000007794 irritation Effects 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000002834 transmittance Methods 0.000 claims abstract description 34
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 31
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 31
- 239000011259 mixed solution Substances 0.000 claims abstract description 14
- 238000010998 test method Methods 0.000 claims abstract description 7
- 239000003599 detergent Substances 0.000 claims description 54
- 239000002453 shampoo Substances 0.000 claims description 22
- 239000002994 raw material Substances 0.000 abstract description 15
- 239000000243 solution Substances 0.000 abstract description 14
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 238000004925 denaturation Methods 0.000 abstract description 4
- 230000036425 denaturation Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 230000035699 permeability Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000008367 deionised water Substances 0.000 description 11
- 229910021641 deionized water Inorganic materials 0.000 description 11
- 230000000694 effects Effects 0.000 description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 3
- 206010015946 Eye irritation Diseases 0.000 description 3
- 239000002537 cosmetic Substances 0.000 description 3
- 235000014103 egg white Nutrition 0.000 description 3
- 210000000969 egg white Anatomy 0.000 description 3
- 231100000013 eye irritation Toxicity 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 206010022998 Irritability Diseases 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229940071167 c14 olefin sulfonate Drugs 0.000 description 1
- 210000003837 chick embryo Anatomy 0.000 description 1
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 description 1
- 229940073507 cocamidopropyl betaine Drugs 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 1
- 229940048848 lauryl glucoside Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229940083542 sodium Drugs 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 229940057950 sodium laureth sulfate Drugs 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- 229940045885 sodium lauroyl sarcosinate Drugs 0.000 description 1
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Cosmetics (AREA)
- Detergent Compositions (AREA)
Abstract
The invention relates to the technical field of daily chemical articles, and provides a method for testing the irritation of a surfactant. Mixing a sample to be detected with egg white protein, standing the obtained mixed solution to obtain a liquid to be detected, and testing the transmittance of the liquid to be detected by using an ultraviolet spectrophotometer; the higher the transmittance of the liquid to be measured, the lower the irritation of the sample to be measured. After the surfactant is contacted with egg white protein, the protein is denatured to different degrees, the higher the denaturation degree is, the more turbid the solution becomes, and then the permeability of the liquid to be tested is utilized to represent the denaturation degree of the protein, so that the irritation of the surfactant is reflected. The test method provided by the invention has simple steps and easy operation, and can quickly and conveniently evaluate the irritation of the surfactant raw material or formula. In addition, the invention can use egg white protein to test the irritation of the surfactant, and has wide raw material source and low cost.
Description
Technical Field
The invention relates to the technical field of daily chemical articles, in particular to a method for testing the irritation of a surfactant.
Background
In the preparation of daily chemical products such as shampoo, liquid detergent, cosmetics and the like, the most commonly used raw material is a surfactant. Irritation assessment is commonly encountered during material screening and formulation assessment, and currently common irritation assessment methods are chick embryo chorioallantoic membrane assay, which is commonly used to test the skin irritation, acute eye irritation, and cosmetic eye irritation/corrosivity of materials, and red blood cell hemolysis, which is commonly used to test the eye irritation of import and export cosmetics. However, the above methods have problems of high test cost, long cycle, and the like, and the irritability of the surfactant raw material or formulation cannot be rapidly evaluated.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for testing the irritation of a surfactant. The method provided by the invention has the advantages of simple steps, easiness in operation and capability of quickly and conveniently evaluating the irritation of the surfactant raw material or formula.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for testing the irritativeness of a surfactant, comprising the steps of:
mixing a sample to be detected with egg white protein, and standing the obtained mixed solution to obtain a liquid to be detected;
testing the transmittance of the liquid to be tested by using an ultraviolet spectrophotometer; the higher the transmittance, the lower the irritation of the sample to be measured; the sample to be detected is a surfactant or a product containing the surfactant.
Preferably, the egg white protein is egg white protein.
Preferably, the mass fraction of the effective surfactant in the mixed solution is 0.1-2.0%.
Preferably, the surfactant-containing product comprises a laundry detergent, a liquid detergent, a body wash or a shampoo.
Preferably, the standing time is 1-3 h, and the standing temperature is 20-25 ℃.
Preferably, the ultraviolet wavelength used in the ultraviolet spectrophotometer test is 550nm, and a transmittance mode is selected for the test.
The invention provides a method for testing the irritation of a surfactant, which comprises the following steps: mixing a sample to be detected with egg white protein, and standing the obtained mixed solution to obtain a liquid to be detected; testing the transmittance of the liquid to be tested by using an ultraviolet spectrophotometer; the higher the transmittance, the lower the irritation of the sample to be measured; the sample to be detected is a surfactant or a product containing the surfactant. According to the method, a sample to be detected and egg white protein are mixed, the protein is denatured to different degrees after a surfactant is contacted with the protein, the liquid to be detected becomes turbid as the denaturation degree is higher, the transmittance of the liquid to be detected is tested by an ultraviolet spectrophotometer, and the denaturation degree of the protein is represented by the transmittance, so that the stimulation of the surfactant is reflected. The test method provided by the invention has simple steps and easy operation, and can quickly and conveniently evaluate the irritation of the surfactant raw material or formula. In addition, the invention can use egg white protein to test the irritation of the surfactant, and has wide raw material source and low cost.
Drawings
FIG. 1 shows the results of the irritation test for various surfactants of example 1;
FIG. 2 shows the results of the irritation test of different laundry detergents of example 2;
FIG. 3 shows the results of the irritation test for different detergents in example 3;
FIG. 4 is the results of the irritation test for the different body washes of example 4;
fig. 5 shows the results of the irritation test of different shampoos of example 5.
Detailed Description
The invention provides a method for testing the irritation of a surfactant, which comprises the following steps:
mixing a sample to be detected with egg white protein, and standing the obtained mixed solution to obtain a liquid to be detected;
testing the transmittance of the liquid to be tested by using an ultraviolet spectrophotometer; the higher the transmittance, the less irritating the sample to be tested.
According to the invention, a sample to be detected and egg white protein are mixed, and the obtained mixed solution is kept stand to obtain a liquid to be detected. In the invention, the sample to be detected is a surfactant or a product containing the surfactant; the surfactant-containing product preferably comprises a laundry detergent, a liquid detergent, a body wash or a shampoo; when the sample to be tested is the surfactant, the testing method can confirm the irritation of different surfactant raw materials, and then a proper raw material composition formula can be selected according to the irritation value; when the test sample is a surfactant-containing product, the irritation of different products can be compared using the method of the present invention.
In the invention, the egg white protein is preferably egg white protein, and the invention takes the egg white protein as a test raw material, thereby further reducing the test cost.
In the present invention, the mass fraction of the effective surfactant in the mixed solution is preferably 0.1 to 2.0%, more preferably 0.5 to 1.5%, and still more preferably 1%.
In a specific embodiment of the invention, the egg white solution is formulated according to the following formula:
(the weight of the sample to be detected and the effective apparent concentration of the sample to be detected)/(the weight of the sample to be detected + the weight of egg white) are 0.1-2.0%;
wherein the effective apparent concentration of the sample to be detected needs to be a known amount or a detectable value; the effective apparent concentration of the invention is mass concentration; in the present invention, the sample to be tested is usually liquid, and if solid, it can be pre-dissolved in a small amount of water.
The sample to be detected and the egg white protein are preferably mixed at room temperature and fully and uniformly mixed.
After the sample to be detected and the protein are mixed, the mixed solution is kept stand to enable the surfactant and the protein to fully react. In the invention, the standing time is preferably 1-3 h, and the standing temperature is preferably 20-25 ℃.
After the liquid to be detected is obtained, the transmittance of the liquid to be detected is tested by using an ultraviolet spectrophotometer. The present invention preferably calibrates the uv spectrophotometer with deionized water, i.e., 100% uv transmittance of water. The irritation of the sample to be detected can be obtained by comparing the transmittance of the liquid to be detected.
The invention has no special requirements on the specific operation method for testing by using the ultraviolet spectrophotometer, and the test can be carried out by using the method well known by the technicians in the field; the wavelength of ultraviolet light used in the test of the ultraviolet spectrophotometer is preferably 550nm, and a transmittance mode is selected for testing. In the invention, the higher the ultraviolet transmittance of the liquid to be detected is, the less irritation the sample to be detected is.
The embodiments of the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Irritation test of surfactant raw materials: the surfactant raw materials tested in this example were sodium dodecylbenzene sulfonate, sodium laureth sulfate (AES), lauryl glucoside, sodium lauroyl sarcosinate, sodium C12-C14 olefin sulfonate, and cocamidopropyl betaine, and the effective surfactant concentrations of the raw materials are shown in table 1, and the ultraviolet spectrophotometer was calibrated with deionized water, with the transmittance of deionized water being 100%.
TABLE 1 surfactant raw materials surface active concentration
Mixing a surfactant and egg white protein according to a formula that (the weight of a sample to be tested): the effective apparent concentration of the sample to be tested)/(the weight of the sample to be tested + the weight of the egg white protein) is 1%, controlling the mass percentage of the surfactant in the obtained mixed solution to be 1%, uniformly mixing, standing for 1h at 25 ℃ to obtain a solution to be tested, testing the transmittance of the solution to be tested by using an ultraviolet spectrophotometer, wherein the wavelength of ultraviolet light is 550nm, and selecting a transmittance mode for testing.
The results obtained are shown in FIG. 1. As can be seen from fig. 1, the irritancy of the above surfactants is, in order from large to small: the method can definitely test the irritation of different surfactants, and can be used for guiding the selection of the surfactant raw materials of daily chemical products in actual production.
Example 2
Laundry detergent irritation test: the laundry detergents tested in this embodiment are seven commercially available laundry detergents of different brands, which are sequentially marked as laundry detergent 1, laundry detergent 2, laundry detergent 3, laundry detergent 4, laundry detergent 5, and laundry detergent 6. The surfactant type and effective surfactant concentration in each laundry detergent are shown in table 2;
TABLE 2 surfactant type and effective surfactant concentration in each of the laundry detergents
The ultraviolet spectrophotometer was calibrated with deionized water, with the deionized water transmittance being 100%.
And (3) mixing the laundry detergent and egg white protein according to a formula of (the weight of the sample to be detected x the effective apparent concentration of the sample to be detected)/(the weight of the sample to be detected + the weight of the egg white protein) being 1% by combining the effective apparent concentration of the laundry detergent, and controlling the mass percentage of the effective components in the obtained egg white solution to be 1%. Standing at 25 deg.C for 1h to obtain solution to be tested, and testing the transmittance of the solution to be tested with ultraviolet spectrophotometer with ultraviolet wavelength of 550nm in transmittance mode.
The results are shown in FIG. 2. According to the figure 2, the irritation of the laundry detergent is that the laundry detergent 3, the laundry detergent 1, the laundry detergent 5, the laundry detergent 6, the laundry detergent 2, the laundry detergent 7 and the laundry detergent 4 are sequentially changed from large to small; the method can definitely test the irritation of the laundry detergents of different brands.
Example 3
And (3) testing the irritation of the detergent: the liquid detergent adopted in this embodiment is seven commercially available liquid detergents of different brands, which are respectively marked as liquid detergent 1, liquid detergent 2, liquid detergent 3, liquid detergent 4, liquid detergent 5, liquid detergent 6 and liquid detergent 7. The surfactant type and effective surfactant concentration in each liquid detergent are shown in table 3;
TABLE 3 surfactant type and effective surfactant concentration in each detergent
The ultraviolet spectrophotometer was calibrated with deionized water, with the deionized water transmittance being 100%.
And mixing the liquid detergent and egg white protein according to a formula that (the weight of the sample to be detected x the effective apparent concentration of the sample to be detected)/(the weight of the sample to be detected + the weight of egg white protein) is 1% in combination with the effective apparent concentration of the liquid detergent, and controlling the mass percentage of the effective components in the obtained mixed solution to be 1%. Standing at 25 deg.C for 1h to obtain solution to be tested, testing the transmittance of the solution to be tested with ultraviolet spectrophotometer, wherein the wavelength of ultraviolet light is 550nm, and testing in transmittance mode.
The results are shown in FIG. 3. According to fig. 3, the irritability of the detergent is as follows from large to small: the detergent 1 is more than the detergent 4, more than the detergent 7, more than the detergent 2, more than the detergent 3, more than the detergent 6 and more than the detergent 5. The method can definitely test the irritation of the liquid detergent of different brands.
Example 4
And (3) testing the irritation of the shower gel: the shower gels tested in this example were two commercially available shower gels of different brands, which were designated as shower gel 1 and shower gel 2, respectively. The surfactant type and effective surfactant concentration in each shower gel are shown in table 4;
TABLE 4 surfactant type and effective surfactant concentration in each shower gel
The ultraviolet spectrophotometer was calibrated with deionized water, with the deionized water transmittance being 100%.
Combining the effective apparent activity concentration of the shower gel, mixing the shower gel and egg white protein according to a formula of (the weight of a sample to be detected x the effective apparent activity concentration of the sample to be detected)/(the weight of the sample to be detected + the weight of the egg white protein) being 1%, and controlling the mass percentage of the effective components in the obtained mixed solution to be 1%. Standing at 25 deg.C for 1h to obtain solution to be tested, testing the transmittance of the solution to be tested with ultraviolet spectrophotometer, wherein the wavelength of ultraviolet light is 550nm, and testing in transmittance mode.
The results are shown in FIG. 4. According to fig. 4, the irritation of the shower gel is as follows: shower gel 1 is more than shower gel 2; the method can definitely test the irritation of the shower gel of different brands.
Example 5
Shampoo irritation test: the shampoos tested in this example are four commercially available shampoos of different brands, which are designated as shampoo 1, shampoo 2, shampoo 3, and shampoo 4. The surfactant type and effective surfactant concentration in each shampoo are shown in table 5;
TABLE 5 surfactant type and effective surfactant concentration in various shampoos
The ultraviolet spectrophotometer was calibrated with deionized water, with the deionized water transmittance being 100%.
Combining the effective apparent activity concentration of the shampoo, mixing the shampoo and egg white protein according to a formula that (the weight of a sample to be detected x the effective apparent activity concentration of the sample to be detected)/(the weight of the sample to be detected + the weight of the egg white protein) is 1%, and controlling the mass percentage of the effective components in the obtained mixed solution to be 1%. Standing at 25 deg.C for 1h to obtain solution to be tested, testing the transmittance of the solution to be tested with ultraviolet spectrophotometer, wherein the wavelength of ultraviolet light is 550nm, and testing in transmittance mode.
The results are shown in FIG. 5. According to fig. 5, the irritation of the shampoo is as follows from big to small: shampoo 1, shampoo 2, shampoo 4 and shampoo 3; the method can definitely test the irritation of shampoos of different brands.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. A method for testing the irritativeness of a surfactant, comprising the steps of:
mixing a sample to be detected with egg white protein, and standing the obtained mixed solution to obtain a liquid to be detected;
testing the transmittance of the liquid to be tested by using an ultraviolet spectrophotometer; the higher the transmittance, the lower the irritation of the sample to be measured; the sample to be detected is a surfactant or a product containing the surfactant.
2. The test method of claim 1, wherein the egg white protein is egg white protein.
3. The test method according to claim 1, wherein the mass fraction of the effective surfactant in the mixed solution is 0.1 to 2.0%.
4. The test method as claimed in claim 3, wherein the surfactant-containing product comprises a laundry detergent, a liquid detergent, a body wash or a shampoo.
5. The test method according to claim 1, wherein the standing time is 1-3 hours, and the standing temperature is 20-25 ℃.
6. The test method of claim 1, wherein the ultraviolet spectrophotometer is used for testing at a wavelength of 550nm in a transmittance mode.
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| CN202010447191.1A CN111650147A (en) | 2020-05-25 | 2020-05-25 | Method for testing irritation of surfactant |
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| CN202010447191.1A CN111650147A (en) | 2020-05-25 | 2020-05-25 | Method for testing irritation of surfactant |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993004369A1 (en) * | 1991-08-19 | 1993-03-04 | Shiseido Company, Ltd. | Method of and kit for testing in vitro stimulation |
| JP2006292686A (en) * | 2005-04-14 | 2006-10-26 | Ajinomoto Co Inc | Evaluation method of surface-active agent |
| CN102168081A (en) * | 2010-12-14 | 2011-08-31 | 四川出入境检验检疫局检验检疫技术中心 | GFP (Green Fluorescent Protein) leakage test method |
| CN102359946A (en) * | 2011-07-26 | 2012-02-22 | 程树军 | Method for evaluating eye irritation by using miniature pig erythrocytes |
-
2020
- 2020-05-25 CN CN202010447191.1A patent/CN111650147A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993004369A1 (en) * | 1991-08-19 | 1993-03-04 | Shiseido Company, Ltd. | Method of and kit for testing in vitro stimulation |
| JP2006292686A (en) * | 2005-04-14 | 2006-10-26 | Ajinomoto Co Inc | Evaluation method of surface-active agent |
| CN102168081A (en) * | 2010-12-14 | 2011-08-31 | 四川出入境检验检疫局检验检疫技术中心 | GFP (Green Fluorescent Protein) leakage test method |
| CN102359946A (en) * | 2011-07-26 | 2012-02-22 | 程树军 | Method for evaluating eye irritation by using miniature pig erythrocytes |
Non-Patent Citations (1)
| Title |
|---|
| 梁治齐等: "《烹饪化学》", 华中科技大学出版社 * |
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Application publication date: 20200911 |