CN111642521A - Disease and pest resistant composition containing hypersensitive protein and application thereof - Google Patents
Disease and pest resistant composition containing hypersensitive protein and application thereof Download PDFInfo
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- CN111642521A CN111642521A CN202010516575.4A CN202010516575A CN111642521A CN 111642521 A CN111642521 A CN 111642521A CN 202010516575 A CN202010516575 A CN 202010516575A CN 111642521 A CN111642521 A CN 111642521A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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Abstract
The invention relates to the technical field of pesticides, and more specifically provides a disease and pest resistant composition containing a hypersensitive protein, which comprises an active component A and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of amino-oligosaccharin, lentinan, nitenpyram, pymetrozine, imidacloprid, acetamiprid, dicyclanil, ethiprole, butene-fipronil, mandipropamid, fluopicolide, boscalid, fluopyram, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, pyraclostrobin, allicin and ethylicin, the weight ratio of the active component A to the active component B is 100: 1-1: 100, respectively; the amino-oligosaccharin includes high molecular weight amino-oligosaccharin and low molecular weight amino-oligosaccharin.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly provides a disease and insect resistant composition containing a hypersensitive protein and application thereof.
Background
In the actual process of agricultural production, fungi are the most important plant pathogen group, and many important diseases such as downy mildew, late blight, phytophthora parasitica and the like are caused by the fungi. Plant viruses are second only to fungal pathogens. The virus is a living body of non-cell shape and parasitism consisting of a nucleic acid molecule (DNA or RNA) and protein, and has strong infection activity and destructive power. Plant viruses, as a parasitic organism of plants, pose a great threat to the growth and development of plants and to human survival and development. Currently, there are over 1000 types of plant viruses that have been studied and named, many of which are important crop pathogens. Statistically, annual losses of plant viruses are in the region of $ 600 billion worldwide.
The Harpin protein is obtained by fermenting engineering bacteria containing natural Harpin protein genes, is easy to be identified by a plant surface receptor, can activate the conduction of endogenous signals in a plant body, and is a broad-spectrum plant function activator. The amino-oligosaccharin is a biological pesticide developed earlier, and in the interaction between plants and pathogenic bacteria, cell wall degrading enzymes secreted by the pathogenic bacteria release oligosaccharide signal molecules by hydrolyzing plant cell walls, and transmit infected information to the plants at an extremely low concentration, so that the expression of plant-encoded phytoalexin and other defense compound synthetase genes is induced, and the defense reaction of plant cells is started. Lentinan is an effective active component extracted from high-quality lentinus edodes fruiting bodies, the active component in the lentinan is beta- (1-3) -D-glucan with branches, a main chain consists of beta- (1-3) -connected glucosyl, and the glucosyl connected with the beta- (1-6) is randomly distributed along the main chain and is in a comb-shaped structure. Lentinan has the effects of immunity and disease resistance, can passivate virus infection activity, inhibit virus proliferation, enhance plant resistance and obviously improve the quality and yield of crops.
At present, pesticide application is the most effective means for preventing and treating crop fungi or virus diseases, but the existing pesticide products can only prevent and treat single plant diseases, different pesticide varieties are often adopted for preventing and treating different diseases respectively, the prevention and treatment effect is poor, the prevention and treatment period is long, the cost is high, and serious environmental pollution and disease resistance can be caused. The invention patent with application publication number CN109744243A discloses an amino-oligosaccharin compound preparation for preventing and treating tobacco virus diseases, a preparation method and application thereof, wherein the active ingredients are amino-oligosaccharin and one or more of berberine or ningnanmycin or lentinan or hypersensitive protein or glucan sugar or mixed fatty acid. Therefore, there is a need to develop a pesticide product which can comprehensively control various plant diseases, especially plant virus diseases, has an excellent control effect, is stable in storage and can be stored for a long time.
Disclosure of Invention
In order to solve the technical problems, the invention provides a disease and pest resistant composition containing hypersensitive protein, which comprises an active component A and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of amino-oligosaccharin, lentinan, nitenpyram, pymetrozine, imidacloprid, acetamiprid, dicyclanil, ethiprole, butene-fipronil, mandipropamid, fluopicolide, boscalid, fluopyram, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, pyraclostrobin, allicin and ethylicin, the weight ratio of the active component A to the active component B is 100: 1-1: 100, respectively;
the amino-oligosaccharin includes high molecular weight amino-oligosaccharin and low molecular weight amino-oligosaccharin.
As a preferred technical scheme of the invention, the Harpin protein comprises a Harpin protein with higher homology with HarpinEccs protein.
As a preferred technical scheme of the invention, the Harpin protein also comprises Harpineccs fusion protein with Harpin functional activity and other Harpin fusion proteins which are obtained by a gene recombination mode.
As a preferred technical scheme of the invention, the high molecular weight amino-oligosaccharin is amino-oligosaccharin with molecular weight of 2000-3000 Da; the low molecular weight amino-oligosaccharin is amino-oligosaccharin with the molecular weight of 800-1000 Da.
As a preferable technical scheme of the invention, the weight ratio of the high molecular weight amino-oligosaccharin to the low molecular weight amino-oligosaccharin is (1.1-1.5): 1.
in a preferred embodiment of the present invention, the disease or pest resistant composition is prepared from an active ingredient and an adjuvant in a form acceptable to agricultural chemicals.
As a preferable technical scheme of the invention, the auxiliary agent is selected from one or a combination of a plurality of dispersing agents, wetting agents, antifreezing agents, thickening agents, emulsifying agents, defoaming agents, disintegrating agents, preservatives and fillers.
As a preferable technical scheme of the invention, the dispersing agent is selected from one or a combination of more of calcium lignosulfonate, sodium lignosulfonate, polyvinylpyrrolidone, sodium methylene bis-naphthalene sulfonate, naphthalene formaldehyde condensation polymer sulfonate, polycarboxylic acid, polyoxyethylene polyoxypropylene ether block copolymer and fatty alcohol polyoxyethylene ether phosphate; the average molecular weight of the polyoxyethylene polyoxypropylene block copolymer is 2800-3000.
As a preferable technical scheme, the preparation form is any one of a suspending agent, an oil suspending agent, a microcapsule suspending agent, a suspoemulsion, an emulsion in water, a microemulsion, a wettable powder, a water dispersible granule, a water aqua, a soluble powder, a soluble tablet, a soluble granule, a soluble solution, a micro granule, a powder, a wettable granule and a seed coating agent.
The second aspect of the invention provides an application of the disease and pest resistant composition containing the hypersensitive protein in controlling diseases and pests of melons, fruits, vegetables, tobacco, rapes, rice and corn.
Has the advantages that:
(1) the invention provides a disease and pest resistant composition containing hypersensitive protein, Harpin protein does not directly kill or inhibit the growth of pathogenic organisms in the action process, can be contacted with cell surface receptors on plant leaves or root systems as an exciton to send out a pathogen attack alarm, then activates the activity of intracellular related enzymes and protein phosphorylation through configuration change to form a second messenger, amplifies signals, finally stimulates plants to generate defense reaction through regulating special genes, and can greatly enhance the defense capability of crops to various adverse environmental hazards such as viruses, bacteria, fungi, drought, high temperature, frost damage and the like; the damage of crops to diseases and various adverse environments is excited from the inside, so that strong resistance and restoration capability can be generated, regeneration and recovery can be promoted quickly, and the damage of the crops caused by disasters can be greatly reduced or eliminated; the traditional pesticide achieves the control effect by entering plants and pathogens to kill or prevent the growth of the pathogens, the drug resistance and the drug resistance of the pathogens are easily and quickly induced, while the Harpin protein is a natural protein which can activate the endogenous signal conduction in the plants, does not change the biological structure of the pathogens, does not cause the drug resistance of the plant pathogens after long-term or repeated induction, does not kill pests and beneficial organisms, is extremely easy to decompose in the natural environment, is safe and harmless, is environment-friendly, and accords with the international development prospect;
(2) the Harpin protein and the active component B of the composition have different action mechanisms, can directly inhibit plant diseases, can activate the immunocompetence of plants, improve the resistance of the plants to the diseases, reduce the application amount of chemical pesticides, can be applied to preventing and treating downy mildew, phytophthora blight, anthracnose, early blight, rice blast, wheat powdery mildew, virus diseases, bacterial angular leaf spot, basal rot, soft rot, ulcer diseases, bacterial wilt diseases, sclerotinia and the like of crops such as melons, vegetables, tobaccos, rapes, rice, corn and the like, has broad-spectrum plant disease prevention and treatment effects, and particularly has obvious plant virus disease prevention and treatment effects;
(3) the composition has excellent storage stability and durability by compounding the specific active component and the adjuvant, can exert respective drug effects of the Harpin protein and the active component B to the maximum extent, greatly improves the activity of the medicament, greatly reduces the using dose and the using cost of the medicament, and has wide development prospect.
Detailed Description
The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.
The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.
In order to solve the technical problems, the invention provides a disease and pest resistant composition containing hypersensitive protein, which comprises an active component A and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of amino-oligosaccharin, lentinan, nitenpyram, pymetrozine, imidacloprid, acetamiprid, dicyclanil, ethiprole, butene-fipronil, mandipropamid, fluopicolide, boscalid, fluopyram, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, pyraclostrobin, allicin and ethylicin, the weight ratio of the active component A to the active component B is 100: 1-1: 100, respectively;
the amino-oligosaccharin includes high molecular weight amino-oligosaccharin and low molecular weight amino-oligosaccharin.
In a preferred embodiment, the weight ratio of active component a to active component B is 50: 1-1: 50; more preferably, the weight ratio of the active component A to the active component B is 3: 7.
active component A
The active component A comprises Harpin protein.
The Harpin protein is a multifunctional protein encoded by a gram-negative plant pathogenic bacterium hrp (hypersensitive infection and pathophysiology gene), is rich in glycine (Gly), lacks cysteine (Cys), is stable under heat and is sensitive to protease.
In a preferred embodiment, the Harpin protein comprises a Harpin class protein with high homology to HarpinEccs proteins, such as HarpinEcc, HarpinEa, HarpinEch, Harpinpst, Harpinpsg, Harpinpss, and the like.
In a preferred embodiment, the Harpin protein further comprises HarpinEccs fusion protein and other Harpin fusion proteins with Harpin functional activity obtained by gene recombination, such as HarpinEccs + HarpinEcc, HarpinEccs + HarpinEa, HarpinEccs + HarpinEch, HarpinEcc + HarpinEca, HarpinEcc + HarpinEch, HarpinEa + HarpinEch, and the like.
The Harpin protein of the present invention is commercially available, including but not limited to, from the science and technology development ltd, agrogan, Hunan.
Active component B
The active component B of the invention comprises a pesticide or an antibacterial agent.
In a preferred embodiment, the insecticide is selected from pymetrozine, cyhalothrin, fluopicolide, fluopyram, boscalid, abamectin, acephate, acetamiprid, mandipropamid, gossypol, aldicarb, allethrin, pirimiphos-methyl, bensulophosphate, baphate, bacillus thuringiensis, carbofuran, transfluthrin, benfurethrin, benfuracarb, sulfofencarb, dimefluthrin, bifenthrin, bioallethrin, resfluthrin, buprofezin, butocarbofuran, carbosulfan, cartap, chlorantraniliprole, phosphorus oxychloride, chlorfenapyr, chlorfluazuron, chlorthion, chlorpyrifos-methyl, chromafenozide, clothianidin, fenide, cyanamide, cyhalodiamide, cycloprothrin, cyhalothrin, lambda-cyhalothrin, cy, Cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, cyphenothrin, cyromazine, deltamethrin, diafenthiuron, diazinon, dichlorvos, diflubenzuron, dimethoate, chlorfenvinphos, dinotefuran, phenetole, disulfoton, emamectin benzoate, prallethrin, endosulfan, alpha-endosulfan, thiophosphine (EPN), S-fenvalerate, ethiofencarb, ethiprole, ethofenprox, etrimfos, fenitrothion, sec-buticarb, fenoxycarb, fenthion, fenvalerate, fipronil, flonicamid, tefluazuron, flufenoxanide, cyfluazuron, flufenpyrad, flufenpyr, flufenoxuron, butene-fipronil, flumethrin, flupirfenidone, tau-fluvalinate, tementhrin, disulfoton, furacarb, chlorfenapyr, fluazu, Any one of hexaflumuron, hydramethylnon, imidacloprid, triazophos, malathion, meperfluthrin, metaflumizone, metaldehyde, chlorfenvinphos, methamidophos, methidathion, methomyl, methoprene, metofluthrin, nitenpyram, novaluron, omethoate, sufosmin, methyl parathion, permethrin, phenothrin, phenthoate, phorate, phoxim, pirimiphos, spinetoram, tefluthrin, terbufos, fenvinphos, tetramethrin, dextrotetramethrin, transfluthrin, fluvalinate, thiacloprid, thiamethoxam, thiocyclam, thiodicarb, monocarb, fosetyl, phorate, trichlorfon, triflumuron and hexaflumuron; the antibacterial agent is selected from amino-oligosaccharide, lentinan, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, amoxicillin, ampicillin, thiochlorophene, bronopol, cefazolin, cefquinome, ceftiofur, chlortetracycline, clavulanic acid, danofloxacin, difloxacin, dinitolmide, enrofloxacin, florfenicol, lincomycin, lomefloxacin, marbofloxacin, milofloxacin, milomycin, trichloromethylpyridine, norfloxacin, octreone, ofloxacin, orbifloxacin, oxolinic acid, oxytetracycline, penicillin, streptomycin, thiamphenicol, thiamethoxam fumarate, tilmicosin phosphate, tylosin acetate, tylosin phosphate, tylosin, valnemulin, clotrimazole, fluvalin, cloxacin, fluvalidamycin, clotrimazole, fluvalin, Any one of pyraclostrobin, allicin and ethylicin.
In a more preferred embodiment, the active component B is selected from any one of amino-oligosaccharin, lentinan, nitenpyram, pymetrozine, imidacloprid, acetamiprid, dicyclanil, ethiprole, butene-fipronil, mandipropamid, fluopicolide, boscalid, fluopyram, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, pyraclostrobin, allicin, and ethylicin.
In one embodiment, the active component B comprises lentinan.
In another embodiment, the active component B comprises an amino-oligosaccharide.
In a preferred embodiment, the amino-oligosaccharins include high molecular weight amino-oligosaccharins and low molecular weight amino-oligosaccharins.
In a more preferred embodiment, the high molecular weight amino-oligosaccharin is an amino-oligosaccharin with a molecular weight of 2000-3000 Da; the low molecular weight amino-oligosaccharin is amino-oligosaccharin with the molecular weight of 800-1000 Da.
In a more preferred embodiment, the high molecular weight amino-oligosaccharin refers to an amino-oligosaccharin with a molecular weight of 3000 Da; the low molecular weight amino-oligosaccharin is amino-oligosaccharin with molecular weight of 1000 Da.
In a more preferred embodiment, the weight ratio of the high molecular weight amino-oligosaccharide to the low molecular weight amino-oligosaccharide is (1.1-1.5): 1; more preferably, the weight ratio of the high molecular weight amino-oligosaccharin to the low molecular weight amino-oligosaccharin is 1.3: 1.
the high molecular weight amino-oligosaccharins can be obtained by commercial purchase, including but not limited to purchase from Sabei Yuansai science and technology Co., Ltd; the low molecular weight amino-oligosaccharin can be obtained by commercial purchase, including but not limited to purchase from Shenzhen, sourced Biotech, Inc.; the lentinan is commercially available, including but not limited to from beijing tri pu grass green plant preparations ltd.
The applicant found that Harpin protein and active ingredient B were added to the system and the weight ratio of active ingredient a to active ingredient B was controlled to 100: 1-1: 100, in particular when the weight ratio of active component a to active component B is 50: 1-1: 50, the Harpin protein can be used as an exciton and can be contacted with a cell surface receptor on a plant leaf or root system to send out a pathogen attack alarm, the activity of intracellular related enzymes and protein phosphorylation are activated through configuration change to form a second messenger, a signal is amplified, finally a plant is stimulated to generate a defense response through regulation of a special gene, the damage of the plant to the disease and various types of adverse environments can be stimulated from inside to generate strong resistance and repair capacity, the regeneration and recovery can be promoted quickly, and the damage to the plant caused by disasters can be greatly reduced or eliminated; the lentinan in the active component B can passivate the virus infection activity, inhibit virus proliferation, enhance the plant resistance and obviously improve the quality and the yield of crops; the amino-oligosaccharin can excite the gene in the plant body to produce chitinase, glucanase, PR protein and the like with disease-resistant effect, has cell activation effect, is beneficial to the recovery of the damaged plant, and can promote the root growth and the seedling growth, enhance the stress resistance of crops and promote the growth and the development of the plants. The active component A and the active component B act on the plant together, not only can directly inhibit plant diseases, but also can activate the immunocompetence of the plant and improve the resistance of the plant to the diseases.
The applicant finds that the addition of amino-oligosaccharin in the system can bring about a better disease-resistant effect, but the active ingredients in the obtained disease-resistant and insect-resistant composition are easy to volatilize when the composition is diluted and placed at room temperature for a long time, and when the amino-oligosaccharin with high molecular weight and low molecular weight is added in the system at the same time, the loss of the active ingredients can be obviously reduced, the storage time of the composition is prolonged, the permeability of the composition acting on the surface of a plant is improved, the release of the active ingredients is faster, and a more obvious disease-resistant effect is brought. The reason is probably that the high molecular weight amino-oligosaccharin can relatively increase the viscosity of the system, can slow down the diffusion and loss speed of small molecules, prolongs the storage time of the composition, and the low molecular weight amino-oligosaccharin can quickly permeate into the inner layer of a plant, so that the composition can better act on plant cells and induce the disease resistance of the plant, thereby generating immunity and killing effects on various fungi, bacteria and viruses and having good control effects on diseases such as wheat mosaic disease, cotton verticillium wilt, rice blast, tomato late blight and the like. In addition, the amino-oligosaccharin also has good film forming property, can interact with Harpin protein to form a layer of protective film on the surface of the amino-oligosaccharin, and can prolong the contact time of the amino-oligosaccharin serving as an exciton and a plant cell surface receptor, so that the defense capability of crops to various adverse environmental hazards such as viruses, bacteria, fungi, drought, high temperature, frost damage and the like can be improved for a long time.
Auxiliary agent
The disease and pest resistant composition is prepared into a pesticide allowable dosage form by active ingredients and auxiliary agents; the formulation allowed on the pesticide is prepared by uniformly mixing and grinding the active ingredient and the auxiliary agent together to prepare the required formulation.
In a preferred embodiment, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, an antifreeze agent, a thickening agent, an emulsifier, a defoaming agent, a disintegrating agent, a preservative and a filler.
In a more preferred embodiment, the dispersing agent is selected from one or more of calcium lignosulfonate, sodium lignosulfonate, polyvinylpyrrolidone, sodium methylene bis-naphthalene sulfonate, naphthalene formaldehyde condensation polymer sulfonate, polycarboxylic acid, polyoxyethylene polyoxypropylene ether block copolymer and fatty alcohol polyoxyethylene ether phosphate;
the wetting agent is selected from one or a combination of more of sodium dodecyl sulfate, sodium lauryl sulfate, nekal, sodium dodecyl benzene sulfonate, sodium diisobutyl sulfonate and fatty alcohol-polyoxyethylene ether;
the antifreeze is one or a combination of more of ethylene glycol, 1, 2-propylene glycol, glycerol, polyethylene glycol and sorbitol;
the thickening agent is selected from one or more of sodium alginate, gum arabic, magnesium aluminum silicate, xanthan gum, polyvinyl alcohol, organic bentonite and white carbon black;
the emulsifier is selected from one or a combination of more of calcium dodecyl benzene sulfonate, styryl phenol polyoxyethylene ether phosphate, benzyl phenol polyoxyethylene ether phosphate, sorbitan trioleate, sorbitan tristearate, polyethylene glycol monostearate, glycerol monostearate, sorbitan monooleate, sorbitan monolaurate, polyoxyethylene lauryl ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene monopalmitate, polyoxyethylene lauryl ether, sorbitan polyoxyethylene ether, polyoxyethylene sorbitan monooleate, styryl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether and castor oil polyoxyethylene ether;
the defoaming agent is selected from one or a combination of a polyether defoaming agent, an organic silicon defoaming agent and a polyether modified polysiloxane defoaming agent;
the disintegrating agent is selected from one or more of sodium carboxymethyl starch, carboxypropyl cellulose and croscarmellose sodium;
the preservative is selected from one or a combination of more of kasong, formaldehyde, sodium benzoate, sorbic acid and potassium sorbate;
the filler is selected from one or a combination of more of ammonium sulfate, sodium chloride, potassium chloride, soluble starch, glucose and urea.
In a preferred embodiment, the formulation is any one of a suspension, an oil suspension, a microcapsule suspension, a suspoemulsion, an aqueous emulsion, a microemulsion, a wettable powder, a water dispersible granule, an aqueous solution, a soluble powder, a soluble tablet, a soluble granule, a soluble solution, a fine granule, a powder, a wettable granule and a seed coating.
Compositions for various dosage forms can be prepared using methods commonly used in the art. The composition may be provided as a finished product in which the ingredients have been mixed together, or it may be provided as a separate formulation in which the ingredients are mixed together immediately prior to use.
The contents of active ingredients and other ingredients in the above dosage forms have no special requirements, and the conventional proportion in the art can be adopted.
In a more preferred embodiment, the dosage form is any one of an aqueous agent, a soluble powder, a soluble tablet, a soluble granule, and a soluble solution.
In a more preferred embodiment, the dosage form is an aqueous or soluble powder.
In a more preferred embodiment, the dosage form is a soluble powder; based on 100 parts by weight of the composition, the soluble powder contains 0.5-50 parts by weight of active ingredients (active component A and active component B), 3-12 parts by weight of dispersing agent, 1-8 parts by weight of wetting agent and filler to make up the balance.
In a more preferred embodiment, the soluble powder contains 10 parts by weight of active ingredients (active component a and active component B), 7 parts by weight of dispersing agent, 5 parts by weight of wetting agent, filler and the balance, based on 100 parts by weight of the composition.
The soluble powder is prepared by uniformly mixing an active ingredient A, an active ingredient B, a dispersing agent, a wetting agent and a filler in a mixing cylinder, crushing the mixture by a jet mill and then uniformly mixing the crushed mixture.
In a more preferred embodiment, the dispersant comprises a polyoxyethylene polyoxypropylene block copolymer and a polycarboxylic acid; the wetting agent is sodium dodecyl sulfate; the filler is glucose.
In a more preferred embodiment, the polyoxyethylene polyoxypropylene block copolymer has an average molecular weight of 2800-3000.
In a more preferred embodiment, the polyoxyethylene polyoxypropylene block copolymer and the polycarboxylic acid are present in a mass ratio of 1: (1.2-1.4); more preferably, the mass ratio of the polyoxyethylene polyoxypropylene block copolymer to the polycarboxylic acid is 1: 1.3.
the polyoxyethylene polyoxypropylene segmented copolymer is purchased from Nantong Zhanyi chemical Co., Ltd, and has the model of L64; the polycarboxylic acid was purchased from Japan bamboo fat and oil Co., Ltd, and was designated YUS-WG 5.
The applicant found that when the amount of amino-oligosaccharin is added more, particularly when the amount of high molecular weight amino-oligosaccharin is added more, the disease resistance effect of the plant is weakened, and when the weight ratio of high molecular weight amino-oligosaccharin to low molecular weight amino-oligosaccharin is controlled to be (1.1-1.5): 1, the disease resistance effect of the plant can be effectively improved, probably because when the high molecular weight amino-oligosaccharin is excessive, the diluted system is easy to have larger viscosity and lower uniformity, and when the high molecular weight amino-oligosaccharin acts on the plant, the effective ingredients are slowly released, so that the plant cannot be well stimulated to generate defense reaction. The applicant finds that the added polyoxyethylene polyoxypropylene block copolymer and polycarboxylic acid can improve the dispersion effect of the system, and simultaneously improve the stability and disease resistance effect of the effective components, probably because the polycarboxylic acid can form stronger acting force with amino-oligosaccharin rich in amino and hydroxyl to improve the dispersion effect; in addition, the polycarboxylic acid has certain acidity, and neutralizes the alkalinity of the amino-oligosaccharin, so that the hydrolysis of effective molecules under the alkalinity is reduced, and the disease-resistant effect is improved.
The applicant unexpectedly found that when a polyoxyethylene polyoxypropylene block copolymer having an average molecular weight of 2800-3000 is selected and the mass ratio of the polyoxyethylene polyoxypropylene block copolymer to the polycarboxylic acid is controlled to be 1: (1.2-1.4) flocculation phenomenon generated after dilution can be improved, probably because protein substances with certain spatial structures are formed by coiling and folding polypeptide chains consisting of amino acids in a dehydration condensation mode, the stability is high, the protein substances are easy to coalesce together in water, strong polarity of polycarboxylic acid can form strong acting force with protein, polyoxyethylene polyoxypropylene block copolymer with average molecular weight of 2800-3000 can provide better steric hindrance effect, and the flocculation phenomenon caused by protein addition is improved by mutual cooperation of the two.
The disease or pest resistant composition of the present invention may contain, in addition to the above-mentioned essential components, a protein protecting agent and a stabilizer as appropriate within a range not to impair the effects of the present invention.
Examples of such components include polyhydroxy compounds, sugars, amino acids, and the like, but are not limited to these.
As examples of the polyol, glycerin, mannitol, sorbitol, inositol, thiol, polyethylene glycol, and the like; examples of the sugar include sucrose, lactose, cellobiose, mannose, maltose, inositol, raffinose, inulin, dextran, maltodextrin, maltopolysaccharide, sucrose octasulfate, heparin, 2-hydroxypropyl- β cyclodextrin, and the like; examples of the amino acid include proline, L-tryptophan, sodium glutamate, alanine, glycine, lysine hydrochloride, sarcosine, L-tyrosine, phenylalanine, and arginine.
The invention provides a disease and pest resistant composition containing the hypersensitivity protein, which is applied to the control of diseases and pests of melons, fruits, vegetables, tobacco, rapes, rice and corn.
The present invention is not particularly limited with respect to the type of pest prevention, for example, the crop diseases caused by the fungus may include downy mildew, late blight, black shank, cotton blight, etc.; the crop diseases caused by the virus can comprise stripe disease, black-streaked dwarf disease, virus diseases and the like.
The disease and pest resistant composition can be applied by adding water according to a common method, the water adding amount can be automatically regulated and controlled according to requirements, and the composition can be used for spray stem leaf treatment, soil treatment, such as solid root spreading or liquid root irrigation, seed dressing, seed soaking or seed coating. The dosage of the above composition for preventing diseases and insect pests per mu of land is not particularly limited, and can be specifically adjusted according to the dosage per mu of active ingredient and the active ingredient content of the preparation.
Examples
In order to better understand the above technical solutions, the following detailed descriptions will be provided with reference to specific embodiments. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. In addition, the starting materials used are all commercially available, unless otherwise specified.
Example 1
Example 1 of the present invention provides an insect disease-resistant composition containing a hypersensitive protein, the insect disease-resistant composition comprising an active component a and an active component B; wherein the active component A comprises Harpin protein, the active component B comprises amino-oligosaccharin, and the weight ratio of the active component A to the active component B is 3: 7; the amino-oligosaccharin includes high molecular weight amino-oligosaccharin and low molecular weight amino-oligosaccharin.
The high molecular weight amino-oligosaccharin is amino-oligosaccharin with the molecular weight of 3000 Da; the low molecular weight amino-oligosaccharin is amino-oligosaccharin with molecular weight of 1000 Da.
The weight ratio of the high molecular weight amino-oligosaccharin to the low molecular weight amino-oligosaccharin is 1.3: 1.
the high molecular weight amino-oligosaccharin was purchased from Saibei science and technology, Inc., Hubei; the low molecular weight amino-oligosaccharin is purchased from the biography limited of Shenzhen.
The anti-pest composition is prepared into a pesticide allowable dosage form by active ingredients and auxiliary agents.
The dosage form is soluble powder; the soluble powder contains 10 parts by weight of active ingredients (active component A and active component B), 7 parts by weight of dispersing agent, 5 parts by weight of wetting agent and filler to make up the balance based on 100 parts by weight of the composition.
Mixing the active component A, the active component B, the dispersing agent, the wetting agent and the filler together in a mixing cylinder uniformly, crushing the mixture by a jet mill, and then mixing the mixture uniformly to prepare the soluble powder.
The dispersing agent comprises polyoxyethylene polyoxypropylene block copolymer and polycarboxylic acid; the wetting agent is sodium dodecyl sulfate; the filler is glucose.
The average molecular weight of the polyoxyethylene polyoxypropylene block copolymer is 2800-3000.
The mass ratio of the polyoxyethylene polyoxypropylene block copolymer to the polycarboxylic acid is 1: 1.3.
the Harpin protein was purchased from science and technology development, Inc. of agricultural Megaco, Hunan; the polyoxyethylene polyoxypropylene block copolymer is purchased from Nantong Zhanyi chemical Co., Ltd, and has the model of L64; the polycarboxylic acid was purchased from Japan bamboo fat and oil Co., Ltd, and was designated YUS-WG 5.
Example 2
Example 2 of the present invention provides an anti-pest composition containing a hypersensitive protein, which is similar to example 1, except that no high molecular weight amino-oligosaccharin is present.
Example 3
Example 3 of the present invention provides an insect and disease resistant composition containing a hypersensitive protein, which is the same as example 1 except that the weight ratio of the high molecular weight amino-oligosaccharide to the low molecular weight amino-oligosaccharide is replaced with 3: 1.
example 4
Example 4 of the present invention provides an anti-pest composition containing a hypersensitive protein, which is similar to example 1, except that no polycarboxylic acid is used.
Example 5
Example 5 of the present invention provides an anti-pest composition containing a hypersensitive protein, which is substantially the same as example 1, except that the polyoxyethylene polyoxypropylene block copolymer has an average molecular weight of 1900, which is available from Nantong Zhanyi chemical Co., Ltd, model number L35.
Example 6
Example 6 of the present invention provides an anti-pest composition containing a hypersensitive protein, which is substantially the same as example 1, except that the polyoxyethylene polyoxypropylene block copolymer has an average molecular weight of 2000, which is commercially available from Nantong Zhanyi chemical Co., Ltd, and is designated as P65.
Example 7
Example 7 of the present invention provides an anti-pest composition containing a hypersensitive protein, which is similar to example 1, except that the polyoxyethylene polyoxypropylene block copolymer is not included.
Performance testing
1. Storage durability test
After the composition prepared in examples 1 to 7 is prepared into a 500-fold diluent and placed in a dust-free open air at room temperature for one week, the mass loss of the composition is calculated and recorded as w, and the calculation formula is as follows:
wherein m is0Mass of the original composition, m1The composition is a mass for one week.
2. System stability test
The composition prepared in the examples 1-7 is prepared into a diluent of 500 times, whether layering and flocculation phenomena exist during dilution is observed, 100 parallel samples are respectively arranged in each example, and the layering and flocculation probabilities, namely the percentage of the number of layering and flocculation in the total number of the test composition, are recorded.
TABLE 1
3. Rape field efficacy test
Test site: shanxi province Hanzhong city Hantai Wuxiang town Wanjiawan village
Basic conditions of the test field: the area is 1.2 mu, the soil texture is two mud (gray soil), the fertility is moderate, the terrain is flat, the irrigation and drainage are convenient, and the periphery is far away from shading. The corn is used as the first crop (variety: sweet corn No. 3, applying 10.0 kg of pure N and P per mu)2O54.0 kg, K206.0 kg).
Test subjects and settings: yangtze river early oil No. 3, random block arrangement, 3 times of repetition, cell length of 6.13 meters, width of 4.60 meters, area of 28.198 square meters, cell area of 0.0423 mu, interval walkway of 70.0cm, repeat walkway of 60.0cm, protection rows of 3.0 meters around, east-west row direction. Sowing in 28 days after 9 months, wherein no-tillage pit digging and spot sowing are adopted in the sowing mode, 11 rows of pits are dug in each cell, 16 pits are dug in each row, and 176 pits are counted in each cell; the seeding amount is 150 g/mu.
The test method comprises the following steps: the disease and pest resistant composition containing the hypersensitive protein prepared in the example 1 is prepared into 500-fold diluent as a test group, clear water and a 'Lvbao' pesticide control group are arranged, and the specific treatment process comprises the following steps:
CK (equal amount of sprayed clear water): spraying until all leaves are wet. "Lvbao" pesticide treatment: after sowing, the root is irrigated with 2000 times of diluent in 25 days, and 150 milliliters of diluent is filled in each nest; irrigating roots for the second time at intervals of 7 days, wherein the dosage and the method are the same as those of the first time. Treatment with the composition of the invention: spraying for the first time: sowing 2-3 true leaves after seedling emergence, and spraying until all leaves are wet; and (3) spraying for the second time: spraying at intervals of 20-25 days until all leaves are wet; and (3) spraying for the third time: during the swelling period of the siliques, spraying until all the siliques are wet sprayed. Other field management measures are the same.
The pest control effect and yield statistics for different treatments are shown in tables 2 and 3.
TABLE 2
TABLE 3
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.
Claims (10)
1. An anti-pest composition containing hypersensitive protein, which is characterized by comprising an active component A and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of amino-oligosaccharin, lentinan, nitenpyram, pymetrozine, imidacloprid, acetamiprid, dicyclanil, ethiprole, butene-fipronil, mandipropamid, fluopicolide, boscalid, fluopyram, tebuconazole, epoxiconazole, cyproconazole, tetraconazole, difenoconazole, hexaconazole, flutriafol, prothioconazole, penconazole, myclobutanil, flusilazole, propiconazole, pyraclostrobin, allicin and ethylicin, the weight ratio of the active component A to the active component B is 100: 1-1: 100, respectively;
the amino-oligosaccharin includes high molecular weight amino-oligosaccharin and low molecular weight amino-oligosaccharin.
2. The disease and pest resistant composition containing Harpin protein according to claim 1, wherein the Harpin protein comprises Harpin-like protein with high homology to HarpinEccs protein.
3. The disease and pest resistant composition containing Harpin protein according to claim 2, wherein the Harpin protein further comprises HarpinEccs fusion protein and other Harpin fusion proteins with Harpin functional activity obtained by means of gene recombination.
4. The disease and pest resistant composition containing the hypersensitive protein according to claim 1, wherein the high molecular weight amino-oligosaccharin is amino-oligosaccharin with a molecular weight of 2000-3000 Da; the low molecular weight amino-oligosaccharin is amino-oligosaccharin with the molecular weight of 800-1000 Da.
5. The disease and pest resistant composition containing harpin protein according to claim 1 or 4, wherein the weight ratio of the high molecular weight amino-oligosaccharin to the low molecular weight amino-oligosaccharin is (1.1-1.5): 1.
6. the disease and pest resistant composition containing harpin protein as set forth in claim 1, wherein the composition is prepared from active component and adjuvant in form of pesticide-allowable dosage form.
7. The disease and pest resistant composition containing hypersensitive protein according to claim 6, wherein said adjuvant is selected from one or more of dispersing agent, wetting agent, antifreezing agent, thickening agent, emulsifying agent, defoaming agent, disintegrating agent, preservative, and filler.
8. The disease and pest resistant composition containing hypersensitive protein according to claim 7, wherein said dispersing agent is selected from one or more of calcium lignosulfonate, sodium lignosulfonate, polyvinylpyrrolidone, sodium methylenedinaphthalene sulfonate, naphthalene formaldehyde condensation polymer sulfonate, polycarboxylic acid, polyoxyethylene polyoxypropylene ether block copolymer, fatty alcohol polyoxyethylene ether phosphate; the average molecular weight of the polyoxyethylene polyoxypropylene block copolymer is 2800-3000.
9. The disease and pest resistant composition containing harpin protein according to claim 6, wherein the formulation is any one of suspending agent, oil suspending agent, microcapsule suspending agent, suspoemulsion, aqueous emulsion, microemulsion, wettable powder, water dispersible granule, aqueous solution, soluble powder, soluble tablet, soluble granule, soluble solution, microgranule, powder, wettable granule, and seed coating agent.
10. The disease and pest resistant composition containing the hypersensitive protein according to any one of claims 1 to 9 is applied to controlling diseases and pests of melons, fruits, vegetables, tobacco, rapes, rice and corn.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114938806A (en) * | 2022-07-27 | 2022-08-26 | 北京金泰毅农作物科技有限公司 | Amino-oligosaccharin and fluopyram composite pesticide and preparation method thereof |
| CN115462376A (en) * | 2021-11-04 | 2022-12-13 | 芮城华农生物化学有限公司 | Efficient insect-resistant antibacterial composition for crops and application thereof |
| CN115486454A (en) * | 2021-11-04 | 2022-12-20 | 芮城华农生物化学有限公司 | Protein plant function regulating composition and application thereof |
-
2020
- 2020-06-09 CN CN202010516575.4A patent/CN111642521A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462376A (en) * | 2021-11-04 | 2022-12-13 | 芮城华农生物化学有限公司 | Efficient insect-resistant antibacterial composition for crops and application thereof |
| CN115486454A (en) * | 2021-11-04 | 2022-12-20 | 芮城华农生物化学有限公司 | Protein plant function regulating composition and application thereof |
| CN114938806A (en) * | 2022-07-27 | 2022-08-26 | 北京金泰毅农作物科技有限公司 | Amino-oligosaccharin and fluopyram composite pesticide and preparation method thereof |
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Inventor after: Tian Minghan Inventor after: Yang Jinhui Inventor after: Li Yuanjian Inventor after: Tian Bao Inventor after: Liu Xingzhi Inventor after: Yang Zhenjun Inventor before: Tian Minghan Inventor before: Yang Jinhui Inventor before: Li Yuanjian Inventor before: Tian Bao Inventor before: Liu Xingzhi Inventor before: Yang Zhenjun |
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Application publication date: 20200911 |