CN111635846A

CN111635846A - Down-pressing open-close type deep sea in-situ culture tank

Info

Publication number: CN111635846A
Application number: CN202010483126.4A
Authority: CN
Inventors: 徐健华; 周东辉; 张桥; 郭大伟
Original assignee: Hangzhou Dianzi University
Current assignee: Hangzhou Dianzi University
Priority date: 2020-06-01
Filing date: 2020-06-01
Publication date: 2020-09-08
Anticipated expiration: 2040-06-01
Also published as: CN111635846B

Abstract

The invention discloses a downward-pressing open-close type deep sea in-situ culture tank which comprises a sealing cover and a tank body, wherein an open-close mechanism is connected between the sealing cover and the tank body, the open-close mechanism comprises an outer guide pipe and an inner guide pipe, a rotary table, a guide rod and a spring, the inner guide pipe, the rotary table, the guide rod and the spring are arranged in the outer guide pipe in a matched manner from top to bottom, a guide opening is formed in the outer guide pipe, the sealing cover is connected with the guide rod through the guide opening, a guide groove type sliding guide rail is arranged on the inner side surface of the outer guide pipe, protrusions in inclined planes are respectively arranged at two ends of the bottom surface of the outer guide pipe, a sliding groove is formed between the guide groove type sliding guide rails, a sliding block is arranged on the outer side surface of the inner guide pipe, a sawtooth-shaped bottom surface is arranged.

Description

Down-pressing open-close type deep sea in-situ culture tank

Technical Field

The invention relates to a deep sea microorganism in-situ culture and collection technology, in particular to a downward-pressing open-close type deep sea in-situ culture tank.

Background

The deep sea biological gene resource is very rich and becomes the focus of international open sea resource competition. The deep-sea organism resources are developed on the basis of organism cognition, and the currently discovered deep-sea organisms are less than 1 percent of the total quantity of the deep-sea organisms. The serious shortage of deep sea research tools and the inconvenient use of research tools are two major reasons for the difficulty in deep sea biological research and gene resource acquisition.

The special environment of the seabed as deep as 6000 meters leads the growth and propagation conditions of the deep-sea microorganisms to be strictly restricted, and the distribution density of the deep-sea microorganisms is unknown, so that a special mode is required for capturing the deep-sea microorganisms. The traditional sampling means is that a seawater collecting device is used for throwing into a deep water area, then sealing and transporting to the sea surface, and the method has extremely low sampling efficiency because the content of microorganisms in the sampled seawater is uncertain. In addition, the culture tank is not sealed in the putting process of the existing deep sea in-situ enrichment culture tank, is easy to be polluted, and is inconvenient to disassemble after being recovered.

Disclosure of Invention

In order to solve the defects of the prior art, realize the sealed putting of the culture tank and improve the opening and closing convenience of the tank cover, the invention adopts the following technical scheme:

the utility model provides a deep sea normal position culture tank of formula of opening and shutting down, includes the closing cap and jar body, is connected with the mechanism that opens and shuts between the closing cap and the jar body, the mechanism that opens and shuts include outer pipe and the inside top-down cooperation of outer pipe set up inner conduit, carousel, guide arm and spring, it has the lead wire to open on the outer pipe, the closing cap passes through the lead wire and is connected with the guide arm, outer pipe medial surface is equipped with guide slot formula sliding guide, its bottom surface both ends are equipped with the bulge that is the inclined plane respectively, constitute the spout between the guide slot formula sliding guide, inner conduit lateral surface is equipped with the slider, the inner conduit bottom surface is the cockscomb structure, be equipped with the draw runner on the carousel, the draw runner top is the inclined plane, cooperate with the cockscomb structure bottom surface of bulge and inner conduit respectively. The inner conduit of the opening and closing mechanism is pressed to complete the opening and closing of the sealing cover, and the efficiency is improved.

And a limiting ring is sleeved above the sliding block and connected with the inner side surface of the outer guide pipe. The limiting ring prevents the inner guide pipe from separating from the outer guide pipe due to the elastic force of the spring reset when the cover is opened.

And a return spring and a spring limiting ring are arranged above the limiting ring from bottom to top in a matching way, and the spring limiting ring is connected with the inner guide pipe. The inner conduit can be ensured to be reset normally after the cover is opened and the inner conduit is pressed.

The rotary disc is provided with a positioning rod, and the positioning rod is in transition fit with the inner cavity at the bottom of the inner catheter. The sliding strip on the rotary table can be ensured to be normally matched with the serrated bottom of the inner guide pipe and the protruding part of the guide groove type sliding guide rail on the inner side surface of the outer guide pipe, and dislocation is avoided.

The bottom of the guide rod is in a stepped shaft shape, the spring is sleeved on the stepped shaft, and the bottom of the outer guide pipe is provided with a through hole corresponding to the stepped shaft. The spring is stressed uniformly, and the phenomenon that the spring is inclined to influence the pressing effect is avoided.

The sealing cover is connected with the guide rod through a sealing cover mechanism, the sealing cover mechanism comprises a sealing cover pressing strip, a screw and a buffer spring, one end of the sealing cover pressing strip is connected with the guide rod, a through hole is formed in the other end of the sealing cover pressing strip, the screw is sleeved with the screw, the screw is in threaded connection with the sealing cover with the top provided with the screw hole, and the buffer spring is sleeved on the screw and is arranged between the sealing cover pressing strip and the sealing cover in a matched mode. The relative distance between the sealing cover and the tank body is adjustable, the error of the covering is adjusted, the tightness of the covering is adjusted, the disassembly is convenient, and the covering has a buffering effect.

The top end of the inner catheter is provided with a pressing handle. The pressing handle is convenient for manual pressing on one hand, and on the other hand, on the diving equipment with the motor screw mechanism, the pressing handle can be pressed through the pressing rod on the motor screw through adjusting the relative position of the culture tank and the motor screw mechanism, so that the pressing action of one or more culture tanks can be more conveniently completed.

The tank body is also provided with an end cover, and the end surface of the end cover is provided with a tank opening matched with the sealing cover. Improve the sealing performance of the culture tank.

The invention has the advantages and beneficial effects that:

make the culture tank can open and shut under water in the deep sea, the many region of microorganism, improved the efficiency of sampling, avoided simultaneously uncapping to put in the in-process, because the culture tank is not sealed and receive the pollution, through pressing the mechanism that opens and shuts, improved the efficiency of the culture tank that opens and shuts, uncap and dismantle etc. after being convenient for retrieve.

Drawings

Fig. 1 is a schematic structural view of the present invention.

Fig. 2 is an internal structure view of the opening and closing mechanism of the present invention.

Fig. 3 is a perspective view of a portion of the structure of the present invention.

FIG. 4a is a cut-away view of a portion of an outer catheter in accordance with the present invention.

Fig. 4b is a schematic view of a portion of the inner conduit of the present invention.

Fig. 4c is a schematic view of the turntable structure of the present invention.

FIGS. 5a and b are structural diagrams of a culture tank and diving equipment according to the present invention.

FIGS. 6a and b are schematic diagrams of the floating of the submerged equipment in which the culture tank of the present invention is placed.

1. The sealing cover comprises a sealing cover pressing strip, 2, screws, 3, a buffer spring, 4, a sealing cover, 5, an end cover, 6, a tank body, 7, an outer guide pipe, 8, a guide opening, 9, an inner guide pipe, 10, a pressing handle, 11, a spring limiting ring, 12, a reset spring, 13, a limiting ring, 14, a sliding block, 15, an alignment rod, 16, a sliding strip, 17, a rotary disc, 18, a guide rod, 19, a spring, 20, a stepped shaft, 21, a sliding groove, 22, a protruding part, 23, a guide groove type sliding guide rail, 24, a guide groove, 25 and a pressing rod.

Detailed Description

The following detailed description of embodiments of the invention refers to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.

As shown in figures 1-4, a downward-pressing open-close type deep sea in-situ culture tank comprises a seal cover 4 and a tank body 6, an open-close mechanism is connected between the seal cover 4 and the tank body 6, the open-close mechanism comprises an outer conduit 7 and an inner conduit 9, a turntable 17, a guide rod 18 and a spring 19 which are arranged in the outer conduit 7 in a matched manner from top to bottom, the top end of the guide rod 18 is attached to the bottom of the turntable 17, a guide opening 8 is formed in the outer conduit 7, the seal cover 4 is connected with the guide rod 18 through the guide opening 8 by the seal cover mechanism, the seal cover mechanism comprises a seal cover pressing strip 1, a screw 2 and a buffer spring 3, one end of the seal cover pressing strip 1 is connected with the guide rod 18, the other end of the seal cover pressing strip is provided with a through-hole sleeving screw 2, the screw 2 is connected with the seal cover 4 with a screw hole at the top by screw thread, the buffer spring 3 is sleeved on the screw 2 and, the cover error is adjusted, the tightness of the cover is adjusted, the cover is convenient to disassemble, and the cover has a buffering effect. The internal components of the opening and closing mechanism are integrally higher than the outer conduit 7 after being combined, so that the top of the inner conduit 9 is higher than the outer conduit 7, the sealing cover 4 can be smoothly pressed, and the sealing cover is normally covered. A connecting piece is arranged below the guide opening 8 of the outer guide pipe 7 and is welded with the tank body 6. An end cover 5 is connected on the tank body 6 through threads, and a tank opening matched with the sealing cover 4 is formed in the end face of the end cover 5, so that the sealing performance of the culture tank is improved. The top end of the inner conduit 9 is fixed with the pressing handle 10 through threads, the pressing handle 10 is convenient to manually press on one hand, and on the other hand, on diving equipment with a motor screw rod, the pressing handle can be pressed through adjusting the relative position of the culture tank and the motor screw rod and the pressing rod 25 on the motor screw rod, so that the pressing action of one or more culture tanks can be completed more conveniently.

The inner side surface of the outer guide pipe 7 is provided with a guide groove type sliding guide rail 23, two ends of the bottom surface of the outer guide pipe are respectively provided with a protrusion part 22 which is an inclined plane, a sliding groove 21 is formed between the guide groove type sliding guide rails 23, the outer side surface of the inner guide pipe 9 is provided with a sliding block 14, the bottom surface of the inner guide pipe 9 is in a sawtooth shape, a sliding strip 16 is arranged on the rotary table 17, the top of the sliding strip 16 is in an inclined plane and is respectively matched with the protrusion part 22 and the sawtooth-shaped bottom surface of the inner guide pipe 9, a guide groove 24 of the guide groove type. The pressing, rotating and self-locking movement is realized, so that the downward pressing, opening and closing actions of the sealing cover mechanism are completed, namely the pressing handle 10 is pressed once, the sealing cover 4 is closed, the pressing handle 10 is pressed once again, and the sealing cover 4 is opened. A limiting ring 13 is sleeved above the sliding block 14, and the limiting ring 13 is connected with the inner side surface of the outer guide pipe 7. The stopper ring 13 prevents the inner guide tube 9 from being separated from the outer guide tube 7 by the elastic force of the return spring 19 when the cover is opened. A return spring 12 and a spring limiting ring 11 are arranged above the limiting ring 13 from bottom to top in a matching way, and the spring limiting ring 11 is connected with the inner guide pipe 9. Guarantee that inner tube 9 can normally reset after uncapping and pressing, consider that deep sea environment is more complicated, inner tube 9 in time resets and can avoid the condition such as inner tube 9 that the condition leads to such as deep sea pressure, scale deposit jam, foreign matter winding can not reset again, leads to can't pressing once more after getting into the deep sea sampling region and open closing cap 4. The turntable 17 is provided with an alignment rod 15, and the alignment rod 15 is in transition fit with the bottom inner cavity of the inner conduit 9. The sliding strip 16 on the rotary table 17 can be ensured to be normally matched with the sawtooth-shaped bottom of the inner conduit 9 and the bulge 22 of the guide groove type sliding guide rail 23 on the inner side surface of the outer conduit 7, and dislocation is avoided. The bottom of the guide rod 18 is in a stepped shaft shape, the spring 19 is sleeved on the stepped shaft 20, the bottom of the outer guide pipe 7 is provided with a through hole corresponding to the stepped shaft 20, the diameter of the through hole is larger than that of the stepped shaft 20 and smaller than that of the spring 19, so that when the guide rod 18 is pressed down, the stepped shaft 20 can penetrate through the through hole, and the spring 19 cannot. Due to the arrangement of the stepped shaft 20, the force applied to the spring 19 is uniform, and the spring 19 is prevented from being inclined to influence the pressing effect.

As shown in figures 5 and 6, the sealing cover 4 is kept closed in the process that the culture tank falls to the seabed, the sealing cover 4 is opened after the culture tank falls to the seabed for enrichment culture of microorganisms, and the sealing cover 4 is kept closed in the process of floating after the culture is finished, so that the whole process is pollution-free, and the efficiency is improved. Due to the adoption of the motor scheme, the system can realize accurate control, can adapt to higher requirements and has better reliability. Meanwhile, compared with the scheme that the sealing cover 4 is opened and closed through a tool and is discarded after being used and sunk into the sea bottom, the method does not generate extra garbage in throwing and recovering, and the marine environment is protected.

During operation, the culture tank is built to a preset position by diving equipment, and the following preparation work is needed before entering water:

1. placing meat blocks in a culture tank in advance for attracting and culturing microorganisms;

2. fixing the culture tank at a designated position of the diving equipment;

3. checking whether the transmission of a motor screw rod of the diving equipment is normal;

4. closing the sealing cover 4 of the culture tube, and adjusting the position of a pressure rod 25 of a motor screw rod to be attached to a pressure handle 10 of the culture tank;

5. the weight is attached to the acoustic releaser via a tray.

The opening/closing operation of the lid 4 comprises the following steps:

1. closing process of the cover 4:

pressing the pressing handle 10 to apply a downward pressure to the inner conduit 9, the inner conduit 9 moves along the guide groove 24 of the outer conduit 7 to press the rotary disc 17 to move downward, when the engagement part of the rotary disc 17 and the sliding groove 21 is disengaged, the contact part of the saw teeth at the bottom of the inner conduit 9 and the inclined plane at the top of the upper slide bar 16 generates a radial force to force the rotary disc 17 to rotate, the inclined plane at the top of the slide bar 16 is engaged with the two convex parts 22 and the concave parts between the two convex parts, the end cover 5 and the sealing cover 4 are closed, if the force is removed, the inner conduit 9 is reset along the guide groove 24 of the outer conduit 7 under the action of the reset spring 12, but the sealing cover 4 still keeps the closed state.

2. Opening process of the lid 4:

the pressing handle 10 is pressed again, the inclined plane at the top of the slide bar 16 is separated from the bottom of the guide groove type sliding guide rail 23, the radial force is applied to the inclined plane at the top of the slide bar 16 by the saw teeth at the bottom of the inner guide pipe 9, so that the rotary disc 17 rotates, the inclined plane at the top of the slide bar 16 is aligned with the slide groove 21, after the force is removed, the rotary disc 17 enables the slide bar 16 to slide into the slide groove 21 under the action of the spring 19, and at the moment, the sealing cover 4 is opened.

Open/close principle of culture tank cover 4 after device water entry:

the culture tank is installed on a transverse plate of the diving equipment through bolts, and the pressing action is realized by a motor screw rod, so that the opening/closing action of the culture tank is realized.

The floating principle of the diving equipment is as follows:

after the acoustic releaser receives the instruction of the deck unit, the shackle is opened, the tray connected with the shackle sinks on the sea bottom together with the counterweight, and then the buoyancy of the diving equipment is larger than the gravity so that the equipment floats upwards.

After fishing, the culture tank is taken down from the diving equipment, and the sealing cover 4 can be opened only by pressing the pressing handle 10.

So far, the deep sea in-situ culture tank completes the tasks of culture and sampling.

Through many times of sea tests, samples of deep sea microorganisms are smoothly collected, and the density of the microorganisms in the sea water collected by the culture sampling tank is high, so that abundant samples are provided for subsequent biological research.

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A downward-pressing open-close type deep sea in-situ culture tank comprises a sealing cover (4) and a tank body (6), and is characterized in that an open-close mechanism is connected between the sealing cover (4) and the tank body (6), the open-close mechanism comprises an outer guide pipe (7) and an inner guide pipe (9), a rotary table (17), a guide rod (18) and a spring (19) which are arranged inside the outer guide pipe (7) in a matching way from top to bottom, a guide opening (8) is formed in the outer guide pipe (7), the sealing cover (4) is connected with the guide rod (18) through the guide opening (8), a guide groove type sliding guide rail (23) is arranged on the inner side surface of the outer guide pipe (7), protrusions (22) which are inclined planes are respectively arranged at two ends of the bottom surface of the outer guide pipe (7), a sliding groove (21) is formed between the guide groove type sliding guide rails (23), a sliding block (14) is arranged on the outer side surface, the top of the sliding strip (16) is an inclined plane and is respectively matched with the convex part (22) and the sawtooth-shaped bottom surface of the inner guide pipe (9), the guide groove (24) of the guide groove type sliding guide rail (23) is correspondingly matched with the sliding block (14), and the sliding groove (21) is correspondingly matched with the sliding strip (16).

2. The deep sea in-situ culture tank capable of being opened and closed by pressing down as claimed in claim 1, wherein a limiting ring (13) is sleeved above the sliding block (14), and the limiting ring (13) is connected with the inner side surface of the outer conduit (7).

3. The deep sea in-situ culture tank of the push-down open type according to claim 2, wherein a return spring (12) and a spring retainer ring (11) are arranged above the retainer ring (13) from bottom to top in a matching manner, and the spring retainer ring (11) is connected with the inner guide pipe (9).

4. The deep sea in situ culture tank of the push-down open type according to claim 1, wherein the rotary plate (17) is provided with a positioning rod (15), and the positioning rod (15) is in transition fit with the bottom inner cavity of the inner conduit (9).

5. The deep sea in-situ culture tank of the push-down open type according to claim 1, wherein the bottom of the guide rod (18) is in a stepped shaft shape, the spring (19) is sleeved on the stepped shaft (20), and the bottom of the outer guide pipe (7) is provided with a through hole corresponding to the stepped shaft (20).

6. The deep sea in-situ culture tank capable of being opened and closed by pressing down as claimed in claim 1, wherein the cover (4) is connected with the guide rod (18) through a cover mechanism, the cover mechanism comprises a cover pressing strip (1), a screw (2) and a buffer spring (3), one end of the cover pressing strip (1) is connected with the guide rod (18), the other end of the cover pressing strip is provided with a perforated sleeve screw (2), the screw (2) is in threaded connection with the cover (4) provided with a screw hole at the top, and the buffer spring (3) is sleeved on the screw (2) and is arranged between the cover pressing strip (1) and the cover (4) in a matching manner.

7. The deep sea in-situ culture tank of the push-down open type according to claim 1, wherein the top end of the inner guide tube (9) is provided with a push handle (10).

8. The deep sea in-situ culture tank capable of being opened and closed by pressing down as claimed in claim 1, wherein the tank body (6) is further provided with an end cover (5), and the end surface of the end cover (5) is provided with a tank opening matched with the sealing cover (4).