CN113913280B - Suspended deep sea microorganism enrichment device capable of being automatically capped - Google Patents
Suspended deep sea microorganism enrichment device capable of being automatically capped Download PDFInfo
- Publication number
- CN113913280B CN113913280B CN202111242441.9A CN202111242441A CN113913280B CN 113913280 B CN113913280 B CN 113913280B CN 202111242441 A CN202111242441 A CN 202111242441A CN 113913280 B CN113913280 B CN 113913280B
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- shell
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- 244000005700 microbiome Species 0.000 title claims abstract description 32
- 238000007789 sealing Methods 0.000 claims abstract description 46
- 210000004907 gland Anatomy 0.000 claims abstract description 27
- 239000000725 suspension Substances 0.000 claims abstract description 3
- 230000005540 biological transmission Effects 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000007906 compression Methods 0.000 claims description 8
- 230000006835 compression Effects 0.000 claims description 7
- 238000010168 coupling process Methods 0.000 claims description 6
- 238000005859 coupling reaction Methods 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 5
- -1 includes screw (1) Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 229910001069 Ti alloy Inorganic materials 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000011065 in-situ storage Methods 0.000 abstract description 8
- 239000013535 sea water Substances 0.000 description 7
- 238000007667 floating Methods 0.000 description 5
- 238000011084 recovery Methods 0.000 description 5
- 238000005070 sampling Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000033001 locomotion Effects 0.000 description 3
- 230000005484 gravity Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/22—Settling tanks; Sedimentation by gravity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a suspended deep sea microorganism enrichment device capable of automatically sealing a cover, which belongs to the field of microorganism enrichment and comprises a propeller, a flow guide body, a cover transfer mechanism, a culture tank and a connecting shell, wherein the flow guide body is arranged at the upper part of the connecting shell and is arranged at the outer side of the propeller, the culture tank is arranged at the lower part of the connecting shell, the cover transfer mechanism is arranged in the connecting shell, the upper end of the cover transfer mechanism is connected with the propeller, and the lower end of the cover transfer mechanism is connected with an upper gland. The invention can automatically seal and sample, has simple structure, flexible and convenient suspension and is simultaneously suitable for in-situ enrichment of microorganisms at different depths.
Description
Technical Field
The invention relates to the field of microorganism enrichment, in particular to a suspended deep sea microorganism enrichment device capable of being automatically capped.
Background
The existing deep sea microorganism in-situ enrichment device is in a closed state at the beginning, the device is placed at a designated position in the deep sea, a hydraulic device in a shell drives a hydraulic rod to open an end cover, the device is exposed to seawater, and after the set time is reached, the hydraulic device drives the hydraulic rod to close the end cover to seal the device.
According to the existing deep sea microorganism uncapping type culture cabin, the end cover of the culture cabin body is rotatably jacked through the deep sea motor assembly, microorganism enrichment culture is carried out in a completely opened state, and in the process of arrangement and recovery, the end cover of the culture cabin body is closed to realize the sealing of the microorganism culture cabin body. The invention can realize the enrichment culture of the microorganism in the deep sea in-situ state.
The problems with both of the above devices are: the circuit control is needed, and the structure is complex.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the suspended deep-sea microorganism enrichment device capable of automatically sealing and covering, which can automatically seal and sample, has a simple structure, is flexible and convenient to suspend, and is suitable for full-sea deep microorganism in-situ enrichment.
The technical scheme of the invention is as follows: the utility model provides a but deep sea microorganism enrichment device of automatic closing cap of suspension, includes screw, baffle, closing cap transfer mechanism, cultivates jar and coupling shell, the upper portion of coupling shell is equipped with the baffle, the baffle sets up in the outside of screw, the lower part of coupling shell is equipped with cultivates jar, closing cap transfer mechanism sets up in the inside of coupling shell, closing cap transfer mechanism's upper end is connected with the screw, closing cap transfer mechanism's lower extreme is connected with last gland.
The sealing cover transfer mechanism comprises a screw rod, a guide rail rod, screw rod nuts, a connecting rod and an upper gland, wherein two ends of the screw rod penetrate through bearings arranged in a connecting shell, the upper end of the screw rod is connected with a screw propeller, the screw rod is connected with the screw rod nuts in a threaded mode, the guide rail rod penetrates through the screw rod, two ends of the guide rail rod are fixed on the connecting shell in a limiting mode through shaft retainer rings, the screw rod nuts are connected with the upper end of the connecting rod, the lower end of the connecting rod is connected with the upper gland, and the upper gland is arranged above a tank opening of a culture tank.
The lower extreme of connecting rod is equipped with the connecting rod lid, be connected through compression spring between last gland and the connecting rod lid, the top of going up the gland is equipped with the guide post, compression spring cover is on the guide post.
The upper end of the screw rod is in fit transmission with the screw propeller through a key, and the connecting rod is fixedly connected with the screw rod nut through threads.
The connecting shell is characterized in that a limiting buckle is arranged on the inner side of the connecting shell and connected with the connecting shell through a bolt, a spring is arranged in the buckle and can push the buckle to one side, and the limiting buckle is connected with a limiting groove on the connecting rod in a clamping mode.
The flow guiding body is in a horn shape or a funnel shape, and the water inlet caliber of the flow guiding body is larger than the water outlet caliber.
The culture tank comprises a tank body, a sealing cover, sealing gaskets and culture tubes, wherein the tank body is connected with a connecting shell, a plurality of culture tubes are arranged in the tank body, the sealing cover is arranged above the tank body, the sealing cover is positioned below the upper pressing cover, and the sealing gasket is arranged on the sealing cover.
The culture tank is made of titanium alloy or polytetrafluoroethylene material.
The culture tank and the flow guide body are fixedly connected with the connecting shell through bolts.
One side of the connecting shell is connected with the clamp body through a bolt, and the clamp body is connected with a cable.
The invention relates to a special device for in-situ enrichment and sampling of suspended microorganisms, which is suitable for the enrichment and sampling of microorganisms with different sea depths within 6000 meters in vertical sections and can be carried with a plurality of sets of devices according to requirements. When seawater is put in, the culture tank is opened, and different kinds of microorganism culture mediums can be installed in the tank, so that conditions are provided for microorganism enrichment. In the device floating recovery process, the culture tank opening can be automatically sealed by using the ingenious spiral transmission mechanism of the device, so that the reverse flow of the seawater is prevented, the electric control is not needed, the structure is simple, the cost is low, and the device can be put into the sea for a long time for use. The device can be arranged outside the frame or fixed on a ship anchor rope, and can realize microorganism in-situ enrichment by keeping the vertical state.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of the structure of the present invention;
FIG. 3 is a schematic diagram of the structure of the present invention;
FIG. 4 is a schematic view of a closure transfer mechanism of the present invention;
FIG. 5 is a schematic view of the structure of the culture tank of the present invention;
In the figure: 1-screw, 2-current-conducting body, 3-closing cap transfer mechanism, 4-coupling shell, 5-culture tank, 6-anchor clamps body, 3-1-guide rail pole, 3-2-lead screw, 3-3-lead screw nut, 3-4-spacing buckle, 3-5-connecting rod, 3-6-compression spring, 3-7-connecting rod lid, 3-8-upper gland, 5-1-sealing cap, 5-2-cabin, 5-3-sealing pad, 5-4-culture tube.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
As shown in figures 1-4, the suspended deep sea microorganism enrichment device capable of automatically sealing comprises a propeller 1, a diversion body 2, a sealing cover transfer mechanism 3, a culture tank 5, a connecting shell 4 and a clamp body 6, wherein the upper part of the connecting shell 4 is connected with the diversion body 2 through a bolt, the diversion body 2 is arranged on the outer side of the propeller 1, the lower part of the connecting shell 4 is connected with the culture tank 5 through a bolt, the sealing cover transfer mechanism 3 is arranged in the connecting shell 4, the upper end of the sealing cover transfer mechanism 3 is connected with the propeller 1, the lower end of the sealing cover transfer mechanism 3 is connected with an upper sealing cover 3-8, one side of the connecting shell 4 is provided with a clamp body 6, the clamp body 6 is connected with the connecting shell 4 through a bolt, the clamp body 6 is connected with a cable, and the clamp body 6 is connected with the connecting shell 4 and the anchor cable through bolts, so that the sealing cover can be quickly disassembled and is convenient to use.
The automatic sealing of the lower culture tank is realized by driving a sealing cover transmission mechanism 3 to move through the rotation of a screw 1, the sealing cover transmission mechanism 3 comprises a screw rod 3-2, a guide rail rod 3-1, a screw rod nut 3-3, a connecting rod 3-5, a connecting rod cover 3-7 and an upper gland 3-8, two ends of the screw rod 3-2 penetrate through bearings arranged in a connecting shell 4, the upper end of the screw rod 3-2 is connected with the screw 1 through a key, the screw rod 3-2 is in threaded connection with the screw rod nut 3-3, the guide rail rod 3-1 is arranged in the screw rod 3-2 in a penetrating manner, two ends of the guide rail rod 3-1 are fixed on the connecting shell 4 through shaft check rings in a limiting manner, the upper end of the connecting rod 3-5 is fixedly connected with the screw rod nut 3-3 through threads, the lower end of the connecting rod 3-5 is provided with the connecting rod cover 3-7, the upper gland 3-8 is connected with the connecting rod cover 3-7 through a compression spring 3-6, a guide post is arranged above the upper gland 3-8, and the guide post 3-6 is sleeved on the guide post 3-8 and is arranged above the culture tank.
The inside of the connecting shell 4 is provided with a limiting buckle 3-4, the limiting buckle 3-4 is connected with the connecting shell 4 through a bolt, a spring is arranged in the buckle 3-4 and can push the buckle to one side, and the limiting buckle 3-4 is clamped with a limiting groove on the connecting rod 3-5. When the connecting rod 3-5 and the upper gland 3-8 move downwards to the bottom, the limiting buckle 3-4 is clamped at the limiting groove on the connecting rod 3-5, and the upward movement of the connecting rod 3-5 is limited.
In the recovery process, the upper gland 3-8 compresses the sealing cover 5-1, and the limiting buckle 3-4 is locked, so that the screw nut 3-3 cannot move upwards, and the upper gland 3-8 is prevented from being separated under the unstable impact of ocean currents. Meanwhile, the compression spring 3-6 is fixed between the upper gland 3-8 and the connecting rod cover 3-7, so that the upper gland 3-8 is not only pushed by the screw rod 3-2, but also has the double functions of the elasticity of the compression spring 3-6, and the tank opening of the culture tank 5 is sealed more reliably. When the landing is dismounted, the limit buckle 3-4 is slightly pushed to one side by using an auxiliary tool, the limit buckle 3-4 can be pushed away from the limit groove on the connecting rod, the propeller 1 can be reversely rotated at the moment, and the sealing cover transfer mechanism 3 can be integrally moved upwards.
The upper part of the device is provided with a current-guiding body (optional floating body material), the middle part is provided with a connecting shell and a sealing cover transmission mechanism, the lower part is provided with a culture tank, and the culture tank is fixed with an anchor rope or other equipment frames through a clamp body 6.
The guide body 2 is in a horn shape or a funnel shape, the water inlet caliber of the guide body 2 is larger than the water outlet caliber, in the device floating recovery process, water flows through the guide body, the flow speed and the impact force of the water flow impacting the propeller can be improved, the propeller 1 has larger rotation moment, the upper gland above the culture tank 5 is enabled to rapidly press the sealing gasket, and samples in the tank are protected.
As shown in FIG. 5, the culture tank 5 comprises a tank body 5-2, a sealing cover 5-1, a sealing gasket 5-3 and culture tubes 5-4, wherein the tank body 5-2 is connected with the connecting shell 4 through bolts, a plurality of culture tubes 5-4 are arranged in the tank body 5-2, the sealing cover 5-1 is arranged above the tank body 5-2, the sealing cover 5-1 is positioned below the upper pressing cover 3-8, and the sealing gasket 5-3 is arranged on the sealing cover 5-1. The culture tank 5 is made of titanium alloy or polytetrafluoroethylene material, the whole structure of the culture tank 5 is provided with a floating center which is at the upper part and a gravity center which is at the lower part, and the buoyancy is far greater than the gravity, so that the capability of automatically recovering the initial state when the device is impacted by ocean currents in seawater can be ensured. The culture tank 5 is fixed with the connecting shell through bolts, and is convenient to detach.
When the device is recovered from bottom to top in the sea, the water flow impacts the propeller through the effect of the current guide body, the propeller 1 rotates to drive the screw rod 3-2 to rotate, the screw rod nut 3-3 moves along the guide rail rod 3-1 in an oriented manner relative to the screw rod 3-2, the upper gland 3-8 is fixed together with the screw rod nut 3-3 through the connecting rod 3-5, the screw rod nut 3-3 moves to push the upper gland to move until the sealed culture tank 5 is pressed, and the sealing cover transfer mechanism 3 stops moving. The rotary motion of the propeller 1 is skillfully converted into vertical movement by the cover transfer mechanism 3. The transfer mechanism ensures that the culture tank 5 is sealed quickly in the floating process, seawater does not flow back into the culture tank to pollute the sample, and microorganisms are protected.
The invention relates to a special device for in-situ enrichment and sampling of suspended microorganisms, which is suitable for the enrichment and sampling of microorganisms with different sea depths within 6000 meters and can be carried with a plurality of sets of devices according to requirements. When seawater is put in, the culture tank is opened, and different kinds of microorganism culture mediums can be installed in the tank, so that conditions are provided for microorganism enrichment. In the device floating recovery process, the culture tank opening can be automatically sealed by using the ingenious spiral transmission mechanism of the device, so that the reverse flow of the seawater is prevented, the electric control is not needed, the structure is simple, the cost is low, and the device can be put into the sea for a long time for use. The device can be arranged outside the frame or fixed on a ship anchor rope, and can realize microorganism in-situ enrichment by keeping the vertical state.
Claims (5)
1. The utility model provides a but deep sea microorganism enrichment device of automatic closing cap of suspension, includes screw (1), water conservancy diversion body (2), closing cap transfer mechanism (3), culture tank (5) and coupling shell (4), its characterized in that: the upper part of the connecting shell (4) is provided with a guide body (2), the guide body (2) is arranged at the outer side of the screw propeller (1), the lower part of the connecting shell (4) is provided with a culture tank (5), the sealing cover transmission mechanism (3) is arranged in the connecting shell (4), the upper end of the sealing cover transmission mechanism (3) is connected with the screw propeller (1), the lower end of the sealing cover transmission mechanism (3) is connected with an upper gland (3-8), the sealing cover transmission mechanism (3) comprises a screw rod (3-2), a guide rail rod (3-1), a screw rod nut (3-3), a connecting rod (3-5) and an upper gland (3-8), two ends of the screw rod (3-2) penetrate through bearings arranged in the connecting shell (4), the upper end of the screw rod (3-2) is connected with the screw propeller (1), the screw rod (3-2) is in threaded connection with the screw rod nut (3-3), the guide rail rod (3-1) is arranged in the guide rail rod (3-2) in a penetrating way, the two ends of the connecting rod (3-5) are connected with the upper end of the screw rod (3-3) through the connecting rod (3-4), the lower end of the connecting rod (3-5) is connected with an upper gland (3-8), the upper gland (3-8) is arranged above a tank opening of the culture tank (5), the lower end of the connecting rod (3-5) is provided with a connecting rod cover (3-7), the upper gland (3-8) is connected with the connecting rod cover (3-7) through a compression spring (3-6), a guide post is arranged above the upper gland (3-8), the compression spring (3-6) is sleeved on the guide post, the upper end of the screw rod (3-2) is in matched transmission with the screw propeller (1) through a key, the connecting rod (3-5) is fixedly connected with the screw rod nut (3-3) through a screw thread, the inner side of the connecting shell (4) is provided with a limit buckle (3-4), the limit buckle (3-4) is connected with the connecting shell (4) through a bolt, a spring is arranged in the buckle (3-4), the limit buckle (3-4) is connected with the upper gland (3-5) through a bolt, the limit buckle (3-5) is sleeved on the guide post, the limit buckle (3-5) is connected with the sealing shell (5) through the sealing shell (5), the sealing shell (5) comprises the sealing shell (5) and the sealing shell (5-5), the culture medium is characterized in that a plurality of culture tubes (5-4) are arranged in the cabin body (5-2), a sealing cover (5-1) is arranged above the cabin body (5-2), the sealing cover (5-1) is positioned below the upper gland (3-8), and a sealing gasket (5-3) is arranged on the sealing cover (5-1).
2. A suspended automatically closable deep sea microorganism enrichment device according to claim 1, wherein: the flow guide body (2) is in a horn shape or a funnel shape, and the water inlet caliber of the flow guide body (2) is larger than the water outlet caliber.
3. A suspended automatically closable deep sea microorganism enrichment device according to claim 1, wherein: the culture tank (5) is made of titanium alloy or polytetrafluoroethylene material.
4. A suspended automatically closable deep sea microorganism enrichment device according to claim 1, wherein: the culture tank (5) and the fluid director (2) are fixedly connected with the connecting shell (4) through bolts.
5. A suspended automatically closable deep sea microorganism enrichment device according to claim 1, wherein: one side of the connecting shell (4) is connected with the clamp body (6) through bolts, and the clamp body (6) is connected with a cable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111242441.9A CN113913280B (en) | 2021-10-25 | 2021-10-25 | Suspended deep sea microorganism enrichment device capable of being automatically capped |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111242441.9A CN113913280B (en) | 2021-10-25 | 2021-10-25 | Suspended deep sea microorganism enrichment device capable of being automatically capped |
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| Publication Number | Publication Date |
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| CN113913280A CN113913280A (en) | 2022-01-11 |
| CN113913280B true CN113913280B (en) | 2024-04-30 |
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| CN202111242441.9A Active CN113913280B (en) | 2021-10-25 | 2021-10-25 | Suspended deep sea microorganism enrichment device capable of being automatically capped |
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| CN116602143B (en) * | 2023-07-21 | 2023-09-15 | 山东省林业保护和发展服务中心 | Potted seedling rhizome soil removing equipment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105802833A (en) * | 2016-04-13 | 2016-07-27 | 杭州电子科技大学 | Deep sea in-situ suspension culture tank |
| CN110317723A (en) * | 2019-08-05 | 2019-10-11 | 中国地质大学（北京） | It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank |
| CN111109214A (en) * | 2020-01-19 | 2020-05-08 | 湖南科技大学 | Suction type deep sea benthos collection and in-situ maintenance system and use method thereof |
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2021
- 2021-10-25 CN CN202111242441.9A patent/CN113913280B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105802833A (en) * | 2016-04-13 | 2016-07-27 | 杭州电子科技大学 | Deep sea in-situ suspension culture tank |
| CN110317723A (en) * | 2019-08-05 | 2019-10-11 | 中国地质大学（北京） | It can self-sealing Deep-Sea Microorganisms original position field planting enrichment culture tank |
| CN111109214A (en) * | 2020-01-19 | 2020-05-08 | 湖南科技大学 | Suction type deep sea benthos collection and in-situ maintenance system and use method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 深海微生物原位富集装置设计;宋圆圆等;杭州电子科技大学学报(自然科学版);20170731;第37卷(第4期);第57-60段 * |
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