CN111632635A - Ultrafiltration centrifuge tube device - Google Patents
Ultrafiltration centrifuge tube device Download PDFInfo
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- CN111632635A CN111632635A CN202010640243.7A CN202010640243A CN111632635A CN 111632635 A CN111632635 A CN 111632635A CN 202010640243 A CN202010640243 A CN 202010640243A CN 111632635 A CN111632635 A CN 111632635A
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- cavity
- ultrafiltration
- centrifuge tube
- lower cavity
- collecting pipe
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D19/00—Degasification of liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
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- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The application discloses ultrafiltration centrifuging tube device includes: the device comprises a pipe cover, a filtering cavity, an ultrafiltration membrane, a support sleeve and a collecting pipe; the filtering cavity comprises an upper cavity and a lower cavity; an opening for adding stock solution is arranged on the upper end surface of the upper cavity; the upper cavity is communicated with the lower cavity; the left side and the right side of the lower cavity are symmetrically provided with filter holes; the ultrafiltration membranes are attached to the left side and the right side of the lower cavity and cover the filtering holes; the supporting sleeve is provided with a through hole matched with the shape of the lower cavity; a liquid guide groove for enabling liquid to flow out is arranged on the wall of the through hole; the support sleeve is sleeved on the lower cavity and connected with the upper cavity; the collecting pipe is connected with the supporting sleeve. The invention effectively improves the recovery rate of the target product during ultrafiltration centrifugation, reduces the loss of the target product, and is a high-quality ultrafiltration centrifugal tube device.
Description
Technical Field
The application relates to the technical field of medical equipment, especially, relate to an ultrafiltration centrifuging tube device.
Background
The ultrafiltration centrifugal tube has a rapid ultrafiltration function, can achieve a higher concentration coefficient, and is easy to recover concentrated solution from the combination of diluent and a complex sample. The ultrafiltration centrifugal tube is mainly used for concentrating biological samples containing antigens, antibodies, enzymes, nucleic acids (single-strain or double-strain DNA/RNA samples), microorganisms, eluate and purified samples; purifying macromolecular components in the tissue culture medium extract and the cell lysate, removing primers, linkages or molecular markers from the reaction mixture, and removing proteins prior to High Performance Liquid Chromatography (HPLC); desalting, buffer exchange or diafiltration, etc.
At present, the problems of low recovery rate, poor concentration efficiency and large residual quantity exist in the conventional ultrafiltration centrifugal tube, and therefore the invention provides an ultrafiltration centrifugal tube device.
Disclosure of Invention
The embodiment of the application provides an ultrafiltration centrifuge tube device for target product's recovery rate when effectively having improved the ultrafiltration centrifugation has reduced target product's loss.
In view of this, the present application provides an ultrafiltration centrifuge tube apparatus comprising: the device comprises a pipe cover, a filtering cavity, an ultrafiltration membrane, a support sleeve and a collecting pipe;
the filtering cavity comprises an upper cavity and a lower cavity;
an opening for adding stock solution is formed in the upper end face of the upper cavity;
the upper cavity is communicated with the lower cavity;
the left side and the right side of the lower cavity are symmetrically provided with filter holes;
the ultrafiltration membranes are attached to the left side and the right side of the lower cavity and cover the filtering holes;
the supporting sleeve is provided with a through hole matched with the lower cavity in shape;
a liquid guide groove for enabling liquid to flow out is formed in the hole wall of the through hole;
the support sleeve is sleeved on the lower cavity and connected with the upper cavity;
the collecting pipe is connected with the supporting sleeve.
Optionally, the ultrafiltration membrane and the left and right side walls of the lower cavity are both curved surface structures.
Optionally, the collection tube is removably connected to the support sleeve.
Optionally, the support sleeve is detachably connected with the upper cavity.
Optionally, a convex ring is arranged at the upper end of the supporting sleeve;
the support sleeve is detachably connected with the upper cavity body through the convex ring;
the collecting pipe is positioned below the convex ring.
Optionally, the outer diameter of the upper cavity is equal to the outer diameter of the convex ring;
the outer diameter of the convex ring is equal to the outer diameter of the collecting pipe.
Optionally, an anti-slip structure for facilitating disassembly is arranged on the outer side wall of the collecting pipe.
Optionally, the lower cavity is tapered from top to bottom.
Optionally, the lower cavity is integrally formed with the upper cavity.
Optionally, the upper end of the upper cavity is screwed with the tube cover, the tube cover is provided with air holes, and the inner side of the tube cover is provided with a breathable film.
According to the technical scheme, the embodiment of the application has the following advantages: the device comprises a pipe cover, a filtering cavity, an ultrafiltration membrane, a supporting sleeve and a collecting pipe, wherein the filtering cavity comprises an upper cavity and a lower cavity, an opening for adding stock solution is formed in the upper end face of the upper cavity, the upper cavity is communicated with the lower cavity, filtering holes are symmetrically formed in the left side and the right side of the lower cavity, and the smallest sample loss and the excellent recovery rate can be ensured through smooth and straight-through filtering holes; the ultrafiltration membranes are attached to the left side and the right side of the lower cavity and cover the filtering holes, and the ultrafiltration membranes are directly attached to the left side and the right side of the lower cavity, so that the flow of liquid is facilitated, and high flow rate and extremely high recovery rate can be realized; the supporting sleeve is provided with a through hole matched with the lower cavity in shape, the hole wall of the through hole is provided with a liquid guide groove for enabling liquid to flow out, and the supporting sleeve can protect and attach the ultrafiltration membrane, so that the ultrafiltration membrane can normally work under high-speed centrifugation or long-time centrifugation without being damaged; the supporting sleeve is sleeved on the lower cavity and connected with the upper cavity, and the collecting pipe is connected with the supporting sleeve.
Drawings
FIG. 1 is an exploded view of an ultrafiltration centrifuge tube apparatus according to an embodiment of the present disclosure;
FIG. 2 is a schematic structural diagram of an ultrafiltration centrifugal tube device in the embodiment of the present application after installation;
FIG. 3 is a cross-sectional view of an ultrafiltration centrifuge tube apparatus according to an embodiment of the present application;
FIG. 4 is a schematic diagram of the internal structure of an ultrafiltration centrifugal tube device in the embodiment of the present application;
FIG. 5 is a schematic view of the inner structure of the ultrafiltration centrifugal tube device at another angle in the embodiment of the present application;
wherein the reference numerals are:
1-filtering cavity, 2-ultrafiltration membrane, 3-supporting sleeve, 4-collecting pipe, 11-upper cavity, 12-lower cavity, 31-through hole, 32-convex ring, 41-anti-slip structure, 121-filtering hole and 311-liquid guide groove.
Detailed Description
In order to make the technical solutions of the present application better understood, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
The inventor finds that: people often adopt centrifuge to carry out many times centrifugation concentration to the sample, because the membrane is heat laminating or supersound or bonding on the filter plate, thereby it is limited to lead to the centrifugal force of use owing to have certain clearance between membrane and the backing plate during centrifugation, just so produce easily that the rate of recovery is low, concentration efficiency is poor, the residue is big, the flow is slow and consume resources etc. to be difficult to accord with people's operation requirement.
The present application provides an embodiment of an ultrafiltration centrifuge tube apparatus, which is specifically shown in fig. 1, fig. 3, fig. 4 and fig. 5.
The ultrafiltration centrifuging tube device in this embodiment includes: the pipe cap, filter chamber 1, milipore filter 2, support cover 3 and collecting pipe 4, filter chamber 1 includes cavity 11 and cavity 12 down, the up end of cavity 11 is equipped with the opening that is used for adding the stoste, cavity 11 communicates with cavity 12 down, filter hole 121 has been seted up to the left and right sides symmetry of cavity 12 down, milipore filter 2 laminates in cavity 12's the left and right sides down, and cover filter hole 121, support cover 3 is seted up and is gone up through-hole 31 with cavity 12 shape assorted down, be provided with the liquid guide groove 311 that is used for making liquid outflow on the pore wall of through-hole 31, support cover 3 cover is established on cavity 12 down, and support cover 3 is connected with cavity 11 up, collecting pipe 4 is connected with support cover 3, liquid flows to collecting pipe 4 from the liquid guide groove 311 of support cover 3 after passing through milipore filter 2.
It should be noted that: the device comprises a pipe cover, a filtering cavity body 1, an ultrafiltration membrane 2, a supporting sleeve 3 and a collecting pipe 4, wherein the filtering cavity body 1 comprises an upper cavity body 11 and a lower cavity body 12, an opening for adding stock solution is formed in the upper end face of the upper cavity body 11, the upper cavity body 11 is communicated with the lower cavity body 12, filtering holes 121 are symmetrically formed in the left side and the right side of the lower cavity body 12, and the smallest sample loss and the excellent recovery rate can be ensured through the smooth and straight-through filtering holes 121; the ultrafiltration membranes 2 are attached to the left side and the right side of the lower cavity 12 and cover the filter holes 121, and the ultrafiltration membranes 2 are directly attached to the left side and the right side of the lower cavity 12, so that the flow of liquid is facilitated, and high flow rate and extremely high recovery rate can be realized; the supporting sleeve 3 is provided with a through hole 31 matched with the lower cavity 12 in shape, the hole wall of the through hole 31 is provided with a liquid guide groove 311 for enabling liquid to flow out, and the supporting sleeve 3 can protect and attach the ultrafiltration membrane 2, so that the ultrafiltration membrane 2 can normally work under high-speed centrifugation or long-time centrifugation conditions and is not damaged; the supporting sleeve 3 is sleeved on the lower cavity 12, the supporting sleeve 3 is connected with the upper cavity 11, and the collecting pipe 4 is connected with the supporting sleeve 3.
The above is an embodiment one of the ultrafiltration centrifuge tube apparatus provided in the present application, and the following is an embodiment two of the ultrafiltration centrifuge tube apparatus provided in the present application, specifically referring to fig. 1 to 5.
The ultrafiltration centrifuging tube device in this embodiment includes: the pipe cover, filter chamber 1, milipore filter 2, support cover 3 and collecting pipe 4, filter chamber 1 includes cavity 11 and cavity 12 down, the up end of cavity 11 is equipped with the opening that is used for adding the stoste, cavity 11 communicates with cavity 12 down, filter hole 121 has been seted up to the left and right sides symmetry of cavity 12 down, milipore filter 2 laminates in the left and right sides of cavity 12 down, and cover filter hole 121, support cover 3 and seted up the through-hole 31 that matches with cavity 12 down shape, be provided with the liquid guide groove 311 that is used for making liquid outflow on the pore wall of through-hole 31, support cover 3 cover is established on cavity 12 down, and support cover 3 is connected with cavity 11 up, the inner wall of through-hole 31 laminates with milipore filter 2, collecting pipe 4 is connected with support cover 3, liquid flows to collecting pipe 4 from the liquid guide groove 311 of support cover 3 behind milipore filter 2.
Both sides wall on the left and right sides of milipore filter 2 and lower cavity 12 is the curved surface structure, owing to adopt the design of curved surface structure, and milipore filter 2 passes through the curved surface structure with lower cavity 12 and laminates mutually for increased the surface area of milipore filter 2 in limited structural space, directly increased 2 areas of effectual milipore filter promptly, and then let the single filtration capacity increase.
Collecting pipe 4 can dismantle with supporting cover 3 and be connected, has independent collector, can change according to the needs of experiment, promptly after the experiment, collecting pipe 4 can take out alone, when carrying out the experiment on next step, can select to use a new collecting pipe 4 directly to assemble on supporting cover 3 to can realize the continuity operation.
It should be noted that: the collecting pipe 4 can be connected with the supporting sleeve 3 through threads, and meanwhile, the collecting pipe 4 can be clamped with the supporting sleeve 3 through a clamping structure.
The support sleeve 3 is detachably connected with the upper cavity 11, so that a user can conveniently remove the ultrafiltration membrane 2, the problem that the membrane cannot be taken out for analysis is solved, and meanwhile, the replacement and maintenance are convenient; the ultrafiltration membrane 2 is attached to the separated support sleeve 3 structure, and the ultrafiltration membrane 2 is not damaged by the protection of the attached support sleeve 3, so that the product can be centrifuged at high speed or for a long time.
Specifically, a convex ring 32 is arranged at the upper end of the support sleeve 3, the support sleeve 3 is detachably connected with the upper cavity 11 through the convex ring 32, and the convex ring 32 can be clamped with the upper cavity 11 through a clamping structure; the collector tube 4 is located below the collar 32. The outer diameter of the upper chamber 11 is equal to the outer diameter of the collar 32, the outer diameter of the collar 32 being equal to the outer diameter of the collection tube 4.
The liquid guide grooves 311 are multiple, and the liquid guide grooves 311 are uniformly distributed on the left side and the right side of the hole wall of the through hole 31.
Be provided with on the lateral wall of collecting pipe 4 and be used for conveniently carrying on the anti-skidding structure 41 of dismantling, it is specific, this anti-skidding structure 41 can be anti-skidding line or anti-skidding arch.
The lower cavity 12 is a structure gradually narrowing from top to bottom, a narrow-channel filtering chamber can be formed by the structure gradually narrowing from top to bottom, and concentration polarization can be reduced and the centrifugal speed can be accelerated while the maximum flow speed is realized through the narrow-channel filtering chamber.
The lower cavity 12 and the upper cavity 11 may be an integrally formed structure.
A pipe cover is screwed at the upper end of the upper cavity 11, specifically, an external thread is arranged on the outer side wall of the upper cavity 11, an internal thread corresponding to the external thread is arranged on the inner side wall of the pipe cover, and the upper cavity 11 is connected with the pipe cover through a thread; the air holes are formed in the tube cover, the air permeable film is arranged on the inner side of the tube cover, and the phenomenon that the centrifugal tube bursts due to gas generation in the centrifugal process of materials can be effectively avoided.
This ultrafiltration centrifuging tube device is through components of a whole that can function independently structural design for ultrafiltration centrifuging tube device becomes very nimble, and the easier operation easily retrieves the target product simultaneously. During the use, as long as add stoste in ultrafiltration centrifuging tube device's filtration cavity 1, then screw up the tube cap to adjust centrifuge speed and carry out the centrifugation, after the centrifugation, open the tube cap, retrieve the target product in the filtration cavity 1.
The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (10)
1. An ultrafiltration centrifuge tube apparatus, comprising: the device comprises a pipe cover, a filtering cavity, an ultrafiltration membrane, a support sleeve and a collecting pipe;
the filtering cavity comprises an upper cavity and a lower cavity;
an opening for adding stock solution is formed in the upper end face of the upper cavity;
the upper cavity is communicated with the lower cavity;
the left side and the right side of the lower cavity are symmetrically provided with filter holes;
the ultrafiltration membranes are attached to the left side and the right side of the lower cavity and cover the filtering holes;
the supporting sleeve is provided with a through hole matched with the lower cavity in shape;
a liquid guide groove for enabling liquid to flow out is formed in the hole wall of the through hole;
the support sleeve is sleeved on the lower cavity and connected with the upper cavity;
the collecting pipe is connected with the supporting sleeve.
2. The ultrafiltration centrifuge tube apparatus of claim 1, wherein the ultrafiltration membrane and the left and right side walls of the lower cavity are both curved structures.
3. The ultrafiltration centrifuge tube apparatus of claim 1, wherein the collection tube is removably attached to the support sleeve.
4. The ultrafiltration centrifuge tube apparatus of claim 3, wherein the support sleeve is removably attached to the upper chamber body.
5. The ultrafiltration centrifuge tube device of claim 4, wherein the upper end of the support sleeve is provided with a convex ring;
the support sleeve is detachably connected with the upper cavity body through the convex ring;
the collecting pipe is positioned below the convex ring.
6. The ultrafiltration centrifuge tube apparatus of claim 5, wherein the outer diameter of the upper chamber body is equal to the outer diameter of the convex ring;
the outer diameter of the convex ring is equal to the outer diameter of the collecting pipe.
7. The ultrafiltration centrifuge tube apparatus of claim 1, wherein the outer sidewall of the collection tube is provided with an anti-slip structure for easy disassembly.
8. The ultrafiltration centrifuge tube apparatus of claim 1, wherein the lower chamber is tapered from top to bottom.
9. The ultrafiltration centrifuge tube apparatus of claim 1, wherein the lower chamber body is integrally formed with the upper chamber body.
10. The ultrafiltration centrifuge tube device of claim 1, wherein the tube cap is screwed on the upper end of the upper cavity, the tube cap is provided with air holes, and the inner side of the tube cap is provided with a breathable film.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010640243.7A CN111632635A (en) | 2020-07-06 | 2020-07-06 | Ultrafiltration centrifuge tube device |
| PCT/CN2020/121641 WO2022007228A1 (en) | 2020-07-06 | 2020-10-16 | Ultrafiltration centrifuge tube device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010640243.7A CN111632635A (en) | 2020-07-06 | 2020-07-06 | Ultrafiltration centrifuge tube device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111632635A true CN111632635A (en) | 2020-09-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010640243.7A Pending CN111632635A (en) | 2020-07-06 | 2020-07-06 | Ultrafiltration centrifuge tube device |
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| Country | Link |
|---|---|
| CN (1) | CN111632635A (en) |
| WO (1) | WO2022007228A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022007228A1 (en) * | 2020-07-06 | 2022-01-13 | 广州洁特生物过滤股份有限公司 | Ultrafiltration centrifuge tube device |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2176817Y (en) * | 1993-03-14 | 1994-09-14 | 安金城 | Centrifugal filter |
| US6344140B1 (en) * | 1997-08-11 | 2002-02-05 | Peter Zuk, Jr. | Centrifugal filtration apparatus |
| CN106040331B (en) * | 2016-07-06 | 2018-12-18 | 江苏科华医疗器械科技有限公司 | A kind of not easily damaged ultra-filtration centrifuge tube |
| CN206635329U (en) * | 2017-03-01 | 2017-11-14 | 广州艺得诺生物科技有限公司 | A kind of cell filter for centrifuging tubular type |
| CN206924902U (en) * | 2017-07-03 | 2018-01-26 | 杭州九龄科技有限公司 | One kind purifying enrichment facility |
| CN211159301U (en) * | 2019-10-21 | 2020-08-04 | 杭州科百特过滤器材有限公司 | Centrifugal filter |
| CN111632635A (en) * | 2020-07-06 | 2020-09-08 | 广州洁特生物过滤股份有限公司 | Ultrafiltration centrifuge tube device |
-
2020
- 2020-07-06 CN CN202010640243.7A patent/CN111632635A/en active Pending
- 2020-10-16 WO PCT/CN2020/121641 patent/WO2022007228A1/en active Application Filing
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022007228A1 (en) * | 2020-07-06 | 2022-01-13 | 广州洁特生物过滤股份有限公司 | Ultrafiltration centrifuge tube device |
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| Publication number | Publication date |
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| WO2022007228A1 (en) | 2022-01-13 |
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