CN111632487A - Preparation for reducing livestock and poultry housing odor and preparation method thereof - Google Patents
Preparation for reducing livestock and poultry housing odor and preparation method thereof Download PDFInfo
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- CN111632487A CN111632487A CN202010600728.3A CN202010600728A CN111632487A CN 111632487 A CN111632487 A CN 111632487A CN 202010600728 A CN202010600728 A CN 202010600728A CN 111632487 A CN111632487 A CN 111632487A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/02—Other waste gases
- B01D2258/0266—Other waste gases from animal farms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a preparation for reducing the odor of livestock and poultry houses and a preparation method thereof, wherein the preparation comprises the following substances in parts by weight: 5-15 parts of bacillus subtilis, 5-10 parts of lactic acid bacteria, 5-20 parts of sodium fulvate paste and 65-85 parts of tap water. The content of the bacillus subtilis is 500-1000 hundred million/g of viable bacteria. The viable count of the lactobacillus is 100-200 hundred million/g. The invention has the beneficial effects that: the feed additive does not contain antibiotics, is nontoxic and harmless to animals and animal products, is safe and efficient, and does not generate drug resistance; effectively decompose residual stink protein in animal excrement, has good antibacterial effect, and effectively inhibits the breeding of fly and other worm eggs. Can effectively adsorb ammonia gas and hydrogen sulfide, has good anti-inflammatory and mucosa immunity enhancing functions, and can effectively prevent respiratory diseases of livestock and poultry. Has stable and long-acting components, saves processing cost, reduces the production cost of products, is convenient to use, and can be beneficial to composting of livestock and poultry manure.
Description
Technical Field
The invention relates to a preparation and a preparation method thereof, in particular to a preparation for reducing the odor of a livestock and poultry barn and a preparation method thereof.
Background
With the continuous expansion of the production and operation scale and the continuous improvement of the intensification degree of the animal husbandry, a large amount of malodorous substances are discharged while a large amount of livestock and poultry products are produced, and the malodorous substances contain hydrogen sulfide, ammonia gas, volatile fatty acid, trimethylamine, methane, skatole, mercaptan and other substances which are mixed together to emit unpleasant odor. Seriously harming the health of livestock and poultry, reducing the disease resistance of the livestock and poultry and hindering the exertion of the production performance: but also endangers the health of people, especially of the people who are kept, and the people who reside in the surroundings are also concerned. The long-term release of such malodors into the atmosphere may also result in acid rain, which may pollute the environment.
As is known, the malodorous substances can stimulate olfactory nerves and trigeminal nerves, thereby acting on respiratory centers and influencing the respiratory functions of livestock and poultry. The pungent odor also causes blood pressure and pulse changes, and some have strong toxicity. The harm of the stink to the livestock and poultry is related to the concentration and the action time of the stink, the low-concentration and short-time action generally has no obvious harm, and the high-concentration stink can cause serious influence on the health of the livestock and poultry, but the situation is rare. In actual production, the influence of the malodor on livestock and poultry is often long-term low-concentration effect, so that the livestock and poultry are subjected to chronic poisoning, weak physique, reduced disease resistance and reduced production performance. Therefore, the harm of the malodor to the livestock and poultry cannot be ignored.
Research shows that the malodorous substances which are harmful to livestock and poultry mainly comprise ammonia gas, hydrogen sulfide and the like. Ammonia gas is a colorless and strong offensive odor gas, and is mainly produced by decomposing feces and urine with bacteria and enzymes in livestock and poultry houses. Are often dissolved or adsorbed on moist floors, walls and mucous membranes of livestock. The livestock external mucous membrane is stimulated to cause mucous membrane congestion, larynx edema and ammonia gas to enter respiratory tract, which can cause symptoms of cough, trachitis, bronchitis, pulmonary edema hemorrhage, dyspnea, asphyxia and the like, and the ammonia inhaled into the lung can enter blood through alveolar epithelial tissue and be combined with hemoglobin to replace oxygen radical and destroy the oxygen transportation function of the blood, so anemia and tissue hypoxia occur. If a small amount of ammonia is inhaled in a short period of time, it can be absorbed and converted into urea to be discharged out of the body. High concentration ammonia can directly stimulate body tissues to dissolve and necrose, and can also cause central nervous system paralysis, toxic liver disease, myocardial injury, etc. Researches show that chickens are very sensitive to ammonia gas, ammonia with different concentrations can cause different influences on the health of poultry, and when the concentration of NH3 in a chicken house reaches 20 mg/l, the incidence rate of various common diseases and frequently encountered diseases such as coccidiosis and the like is increased suddenly; when the concentration is increased to 50 mg/l, the respiratory rate of the chicken can be reduced, respiratory mucosa congestion and edema of the chicken are caused, even bronchitis, pneumonia, emphysema, central nerve paralysis and the like are caused, the egg yield of the laying hens is reduced, and the weight gain and the feed utilization rate of the chicks are reduced. When the ammonia concentration in the henhouse is higher than 78.3 mg/kg, the laying rate is reduced by 43.1 percent. The content of ammonia in the chicken house is inversely related to the laying rate and is very significant. The concentration of NH3 in the pigsty had no effect on daily feed intake, but the average daily gain decreased with increasing NH3 concentration and the feed to weight ratio increased with increasing NH3 concentration in the pigsty. Hydrogen sulfide is mainly generated by anaerobic degradation of sulfur-containing organic matters in fresh excrement, and particularly, when animals eat high-protein daily ration and the digestion utilization rate is low, the amount of hydrogen sulfide is more. Hydrogen sulfide is a colorless combustible gas with a special rancid odor, and is irritating and suffocating. Mainly stimulates mucous membrane, when the hydrogen sulfide contacts the water on the mucous membrane of the animal, the hydrogen sulfide is quickly dissolved and combined with sodium ions in the mucous membrane to generate sodium sulfide, thereby stimulating the mucous membrane. The sick livestock have symptoms of photophobia, lacrimation, cough, rhinitis, tracheitis, etc. Hydrogen sulfide entering the blood via alveoli can bind to ferric iron of the oxidative cytochrome oxidase, deactivating the enzyme, thereby affecting the cellular oxidation process and causing hypoxia. Livestock and poultry are kept in the environment of low-concentration hydrogen sulfide air for a long time. The constitution is weakened, the disease resistance is reduced, and gastrointestinal diseases, heart failure and the like are easy to occur. And vegetative nerve disorder and polyneuritis can occur. High concentrations of hydrogen sulfide can inhibit the respiratory center, directly leading to death of the animal. Therefore, how to effectively control the stink of the farm is an urgent problem to be solved to ensure the sustainable development of the animal husbandry.
Disclosure of Invention
In order to solve the problems, the invention provides a preparation for reducing the odor of a livestock and poultry colony house and a preparation method thereof.
The technical scheme of the invention is as follows: a preparation for reducing the odor of livestock and poultry houses and a preparation method thereof comprise the following substances in parts by weight: 5-15 parts of bacillus subtilis, 5-10 parts of lactic acid bacteria, 5-20 parts of sodium fulvate paste and 65-85 parts of tap water.
The bacillus subtilis has the viable bacteria content of 500-1000 hundred million/g.
The viable count of the lactobacillus is 100-200 hundred million/g.
The preparation method of the sodium fulvate paste comprises the following steps: pulverizing brown coal, adding sodium hydroxide solution, centrifuging extractive solution, removing residue, activating, centrifuging, removing residue, and concentrating to obtain fulvic acid concentrate.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing lignite with the water content of 30-50% into 40-60 meshes;
step two, the pulverized lignite and sodium hydroxide are easily mixed according to the weight ratio of 1: mixing at a ratio of 8-12, extracting at 50-80 deg.C under 1-2 Pa for 2-4 hr to obtain extractive solution;
step three: separating and deslagging the extract by a horizontal centrifuge, adjusting the pH to 6.5-7.5 by using 1mol/L hydrochloric acid, stirring for 5-10 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 70-85% concentration to obtain fulvic acid sodium paste.
The concentration of the sodium hydroxide solution is 3% -6%.
Compared with the prior art, the invention has the beneficial effects that:
(1) the product does not contain antibiotics, contains no toxic and harmful components to animals and livestock products, is safe and efficient, and does not generate drug resistance;
(2) the bacillus subtilis in the components of the product can effectively decompose odor protein remained in animal excrement, and the secretion of the lactobacillus has good antibacterial effect, so that the breeding of ova such as flies can be effectively inhibited. The fulvic acid has a good adsorption function, can effectively adsorb ammonia gas, hydrogen sulfide and the like volatilized from excrement, has good anti-inflammatory and mucosa immunity enhancing functions, and can effectively prevent respiratory diseases of livestock and poultry. In addition, the fulvic acid has the beneficial functions of the bacillus subtilis, and the compatibility of the two has good complementary and synergistic functions.
(3) The product has stable and long-acting components, wherein the fulvic acid adopts paste, so that the processing cost of drying into powder is saved, the production cost of the product is reduced, and the product can be widely used in animal husbandry.
(4) The product is convenient to use, can be directly sprayed by spraying equipment of a farm or a manual sprayer without adding complex equipment, and can also be mixed in feed to feed animals to play the same role.
(5) The product has some components which have no delivery effect and can be helpful for composting animal manure (bacillus, lactic acid bacteria and humic acid are usually added during animal manure composting).
The specific implementation mode is as follows:
example 1
A preparation for reducing the odor of livestock and poultry houses and a preparation method thereof are prepared by mixing the following substances in parts by weight: 10 parts of bacillus subtilis with the viable bacteria content of 800 hundred million/g, 8 parts of lactobacillus with the viable bacteria number of 150 hundred million/g, 15 parts of sodium fulvate paste and 67 parts of tap water.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing 40% brown coal into 50 meshes;
step two: mixing pulverized lignite with sodium hydroxide with the concentration of 4% according to the weight ratio of 1: 10, and extracting for 3 hours at 70 ℃ and under the pressure of 2 Pa to obtain an extracting solution;
step three: separating and deslagging the extracting solution by a horizontal centrifuge, adjusting the pH value to 7.0 by using 1mol/L hydrochloric acid, stirring for 7 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 80% concentration to obtain fulvic acid sodium paste.
Example 2:
a preparation for reducing the odor of livestock and poultry houses and a preparation method thereof are prepared by mixing the following substances in parts by weight: 5 parts of bacillus subtilis with the viable bacteria content of 1000 hundred million/g, 10 parts of lactic acid bacteria with the viable bacteria number of 200 hundred million/g, 20 parts of sodium fulvate paste and 65 parts of tap water are mixed to obtain the composition.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing 30-mesh lignite into 40 meshes;
step two: mixing the pulverized lignite with 3% -6% sodium hydroxide according to the weight ratio of 1: 8, and extracting for 2 hours at 80 ℃ and under the pressure of 1 Pa to obtain an extracting solution;
step three: separating and deslagging the extracting solution by a horizontal centrifuge, adjusting the pH value to 6.5 by using 1mol/L hydrochloric acid, stirring for 10 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 85% concentration to obtain fulvic acid sodium paste.
Example 3:
a preparation for reducing the odor of livestock and poultry houses and a preparation method thereof are prepared by mixing 15 parts of bacillus subtilis with 500 hundred million/g live bacteria content, 5 parts of lactic acid bacteria with 150 hundred million/g live bacteria content, 5 parts of sodium fulvate paste and 75 parts of tap water.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing 50% brown coal into 60 meshes;
step two: mixing the pulverized lignite with 3% -6% sodium hydroxide according to the weight ratio of 1: 12, and extracting for 4 hours at 50 ℃ and under the pressure of 2 Pa to obtain an extracting solution;
step three: separating and deslagging the extracting solution by a horizontal centrifuge, adjusting the pH value to 7.5 by using 1mol/L hydrochloric acid, stirring for 6 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 75% concentration to obtain fulvic acid sodium paste.
Example 4:
a preparation for reducing the odor of livestock and poultry houses and a preparation method thereof comprise the following substances in parts by weight: 8 parts of bacillus subtilis with 900 hundred million/g of viable bacteria content, 6 parts of lactic acid bacteria with 130 hundred million/g of viable bacteria content, 9 parts of sodium fulvate paste and 77 parts of tap water.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing lignite with the water content of 30 percent into 40 meshes;
step two: mixing pulverized lignite with sodium hydroxide with the concentration of 4% according to the weight ratio of 1: 9, and extracting for 4 hours at 55 ℃ and under the pressure of 2 Pa to obtain an extracting solution;
step three: separating and deslagging the extracting solution by a horizontal centrifuge, adjusting the pH value to 7.5 by using 1mol/L hydrochloric acid, stirring for 8 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 70% concentration to obtain fulvic acid sodium paste.
Example 5
A preparation for reducing the odor of livestock and poultry houses and a preparation method thereof are disclosed, wherein the preparation comprises the following components: comprises the following substances in parts by weight: 5 parts of bacillus subtilis with 700 hundred million/g viable bacteria content, 6 parts of lactic acid bacteria with 150 hundred million/g viable bacteria content, 8 parts of sodium fulvate paste and 81 parts of tap water.
The preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing the brown coal with the water content of 35 percent into 40 meshes;
step two: the pulverized lignite is mixed with 3% of lignite. Sodium hydroxide is easily added in a weight ratio of 1: 11, and extracting for 4 hours at 75 ℃ and under the pressure of 1 Pa to obtain an extracting solution;
step three: separating and deslagging the extracting solution by a horizontal centrifuge, adjusting the pH value to 6.5 by using 1mol/L hydrochloric acid, stirring for 9 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 85% concentration to obtain fulvic acid sodium paste.
The preparation prepared according to the method and the raw materials is tested.
Example 1
Pig experiment:
2 fattening pig houses are selected, 100 pigs are arranged in each house, one is an experimental group, the other is a control group, 2kg/T of the product is added into the daily ration of the experimental group, and the ammonia concentration in the house is measured by a pump suction type hand-held detector (model: AP-S-NH3) of Shenzhen Anpall science and technology Limited company (Shenzhen, the house window is sealed 2 days in advance, and the average value of 8:00, 14:00 and 22:00 is taken on the detection day).
All the following experimental data were analyzed for one-way anova using the sps 17.0 software, and compared for significance using the Duncan formula (P <0.05).
Test result 1, the influence of the product on the growth performance of the fattening pigs and the ammonia gas emission of the piggery is shown in the following table:
as can be seen from the test results in the table above, the growth performance of the two groups is almost not different, the test group is slightly lower but has no significant difference (P > 0.05), but the ammonia concentration in the colony house of the test group is significantly lower than that of the control group (P < 0.01), is lower by 42.2%, and has no influence on death and elimination.
Example 2 beef cattle experiment:
selecting beef cattle 2 columns, each column has 30 heads, and the experimental columns are sprayed with the product once every day in the morning and at night, and the weight is 0.5kg/m3The amount of the active ingredients is sprayed. Tracking ammonia generation capacity of 10 cattle manure per column (1kg of fresh manure is placed in a 30L barrel, sealed by a plastic film, and placed in a 25C constant temperature room for 0h, 4h, 8h, 16h and 24h to measure ammonia concentration in the barrel); feces were collected every 5 days for 5 times, and the average of 5 times was calculated.
The test results show that the ammonia gas in the excrement is mainly released within 0-16h, other complex organic nitrogen substances can be degraded into ammonia gas after fermentation for several days, and compared with a control group, the ammonia gas production capacity in the whole process of a test group is obviously reduced, the reduction amplitude reaches 79%, and the effect is obvious.
Example 3 broiler experiment:
selecting 600 yellow-feathered broiler cocks with average weight of 1 day old, randomly dividing into 2 groups, repeating 6 chickens in each group, repeating 50 chickens in each group, adding 1.5kg/T of the feed into an experimental group, and feeding for 60 days old. And (3) testing chickens (21 days old) and chickens (63 days old), collecting uncontaminated fresh excrement in each feeding fence, sealing urease and uric acid in the excrement to be tested, taking a part of excrement for fermentation, and detecting the emission amount of ammonia gas and hydrogen sulfide in the fermented excrement.
The relative urease activity of each test group was based on the control group, based on the urease activity of the control group being 100. The results show that: the urease activity and uric acid content of 63-day-old broiler chickens in the test group are obviously lower than those of the control group. It is shown that the product can improve the level of nitrogen metabolism in the body and thus reduce the production and release of ammonia.
Table 2:
the results show that: the emission amounts of ammonia gas and hydrogen sulfide of broiler chickens in 63 days in the test group are obviously lower than those of the control group, so that the emission of harmful gas ammonia gas and hydrogen sulfide in the broiler chickens can be reduced, and the emission is corresponding to the result of urease and urea nitrogen in excrement.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Example 4: broiler respiratory disease experiment:
300 AA broilers of 3 weeks old are selected, randomly divided into 2 groups and placed in 2 small henhouses, each group is provided with 5 replicates, and each replicate is provided with 30 replicates and used for mycoplasma gallisepticum infection experiments.
Chicken mycoplasma S6Purchased from the institute of veterinary medicine in China. The strains were passaged 3 times in FM-4 medium and the color change unit was determined to be 109CCU/mL, i.e. the inoculated mycoplasma fluid. The left and right thoracic air sacs of each chicken in the experimental group and the control group are respectively inoculated with 0.5mL of mycoplasma bacterial liquid, the clinical symptoms of the tested chicken, such as spirit, ingestion, drinking, respiration and the like, are inoculated and observed, and the drug administration is carried out for 5 days. The control group and the experimental group are fed with the same daily ration, and the experimental group is sprayed with the product 0.3kg/m once in the morning and at night every day3The spraying amount of (1) is 15 days.
The index of curative effect evaluation, observing and recording the clinical symptoms of each group of chickens every day during the whole test period, and counting the stock quantity after the experiment
Treatment of chronic respiratory disease in chicken with product for reducing housing odor
| Group of | Number of test chickens | Number of onset of disease | Number of survivors | The incidence of disease% | The mortality rate is% |
| Experimental group | 150 | 40 | 120 | 26.6 | 20 |
| Control group | 150 | 100 | 80 | 66.6 | 46.6 |
The experimental result shows that the product has good treatment effect and prevention effect on the artificially induced chicken respiratory tract infection.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (6)
1. A preparation for reducing the odor of livestock and poultry houses and a preparation method thereof are characterized in that: comprises the following substances in parts by weight: 5-15 parts of bacillus subtilis, 5-10 parts of lactic acid bacteria, 5-20 parts of sodium fulvate paste and 65-85 parts of tap water.
2. The preparation for reducing the odor of livestock and poultry houses and the preparation method thereof according to claim 1 are characterized in that: the bacillus subtilis has the viable bacteria content of 500-1000 hundred million/g.
3. The preparation for reducing the odor of livestock and poultry houses and the preparation method thereof according to claim 1 are characterized in that: the viable count of the lactobacillus is 100-200 hundred million/g.
4. The preparation for reducing the odor of livestock and poultry houses and the preparation method thereof according to claim 1 are characterized in that: the preparation method of the sodium fulvate paste comprises the following steps: pulverizing brown coal, adding sodium hydroxide solution, centrifuging extractive solution, removing residue, activating, centrifuging, removing residue, and concentrating to obtain fulvic acid concentrate.
5. The preparation for reducing the odor of livestock and poultry houses and the preparation method thereof according to claim 1 or 4, characterized in that: the preparation method of the sodium fulvate paste comprises the following specific steps:
the method comprises the following steps: crushing lignite with the water content of 30-50% into 40-60 meshes;
step two: the pulverized lignite and sodium hydroxide are easily mixed according to the weight ratio of 1: mixing at a ratio of 8-12, extracting at 50-80 deg.C under 1-2 Pa for 2-4 hr to obtain extractive solution;
step three: separating and deslagging the extract by a horizontal centrifuge, adjusting the pH to 6.5-7.5 by using 1mol/L hydrochloric acid, stirring for 5-10 minutes, and separating and deslagging by 2 procedures of the horizontal centrifuge and a disc centrifuge; obtaining a separation liquid;
step four: concentrating the separated liquid to 70-85% concentration to obtain fulvic acid sodium paste.
6. The preparation for reducing the odor of livestock and poultry houses and the preparation method thereof according to claim 5 are characterized in that: the concentration of the sodium hydroxide solution is 3% -6%.
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- 2020-06-29 CN CN202010600728.3A patent/CN111632487A/en active Pending
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