CN111620441B - Synchronous sewage nitrification and denitrification control method and device - Google Patents
Synchronous sewage nitrification and denitrification control method and device Download PDFInfo
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Abstract
A synchronous sewage nitrification and denitrification control method and a device belong to the field of sewage biological treatment. Aiming at the problems of single monitoring means, difficult environmental control, low total nitrogen removal efficiency, high energy consumption and the like in the existing synchronous nitrification and denitrification process, the invention combines DO on-line monitoring and rapid detection of electron transfer activity, simultaneously monitors aerobic-anoxic environment and microbial activity in a biological reaction tank from macroscopic and microscopic aspects, and creates a good reaction environment for nitrifying and denitrifying microorganisms and improves the biological activity of the nitrifying and denitrification microorganisms by controlling the air volume of a variable frequency fan. The invention has the advantages that the removal rate of ammonia nitrogen reaches more than 90 percent, the removal rate of total nitrogen reaches more than 80 percent, and the synchronous nitrification and denitrification project is in a stable operation state.
Description
Technical Field
The invention belongs to the technical field of biological denitrification of sewage treatment, relates to a synchronous nitrification and denitrification control method and device for sewage, and particularly relates to a synchronous nitrification and denitrification control method and device for sewage based on combined control of dissolved oxygen and electron transfer activity.
Background
In the traditional biological sewage denitrification, under the action of ammoniation bacteria, organic nitrogen in sewage is released into ammonia nitrogen, the ammonia nitrogen is converted into nitrate nitrogen by nitrosobacteria and nitrobacteria under an aerobic condition, and then the nitrate nitrogen is converted into nitrogen by denitrifying bacteria under an anoxic condition, so that the aim of denitrification is fulfilled. Nitrification and denitrification are accomplished by different microorganisms, and the two types of microorganisms have different requirements on environmental conditions (organic matter, DO, alkalinity and the like), so a fractional nitrification-denitrification process is usually adopted to ensure that nitrification and denitrification are accomplished independently (spatially or temporally). Later, scientific research finds that the phenomenon of synchronous nitrification and denitrification can be realized, and the nitrification and denitrification can be completed in the same tank by controlling reaction conditions.
The synchronous nitrification and denitrification are completed by microorganism flocs in an aerobic system and anoxic microorganisms in a membrane together, and compared with the conventional nitrification and denitrification, the synchronous nitrification and denitrification has the following advantages: (1) the process flow is more simplified, and the occupied area of the device is small; (2) the consumption of alkalinity in the reaction process is reduced, and the pH value in the reaction tank can be stabilized; (3) the aeration quantity is less and the energy consumption is low. However, when the two processes of the nitrification reaction and the denitrification reaction are unified in one reaction tank, the following contradictions can also occur, which are mainly expressed in that: (1) the competition between oxygen and nitrate nitrogen as electron acceptors affects the nitrification of ammonia nitrogen and the denitrification of nitrate nitrogen, which is expressed as the contradiction of dissolved oxygen; (2) the contradiction of electron donor, the consumption of carbon source under aerobic condition can cause the lack of electron donor in denitrification, and the denitrification can not be carried out or can not be completely carried out.
Therefore, in order to control the synchronous nitrification and denitrification process, the combined control of one or more indexes such as dissolved oxygen, oxidation-reduction potential, pH and the like is mainly adopted at present. The indexes mainly show the macroscopic state in the reaction tank, have hysteresis, lack the monitoring of the microscopic state of the microorganism, and cannot make relevant adjustment in time; other means for directly detecting the activity of the microorganism, such as viable count, ATP detection, nucleic acid detection and the like, are too long in time consumption, or require professional equipment and have high requirements on the basic quality of detection personnel, so that the requirements on rapidness, simplicity, convenience and flexibility cannot be met.
The nature of nitrification and denitrification is the process of electron and proton transfer. The microbial cell is used as a reactor, and an electron transfer chain of the microbial cell consists of a complex I (NADH dehydrogenase complex), a complex II (succinate dehydrogenase complex), a complex III (cytochrome reductase complex) and a complex IV (cytochrome oxidase complex), wherein NADH passes through the complex I, coenzyme Q, the complex III, cytochrome C and the complex IV in sequence to finally transfer electrons to an electron acceptor, discharge protons and simultaneously generate ATP. The detection of the electron transfer activity of microorganisms can directly reflect the nitrification and denitrification activity of microorganisms in the tank, the existing detection method mainly adopts TTC or INT reagent, the detection process needs to be carried out in a laboratory, the whole process comprises the steps of deoxidization, extraction, two-step centrifugation and the like, the operation is complicated, and the detection cannot be finished by simple equipment on the site of a sewage treatment project.
Therefore, how to solve the above problems is an important research content for those skilled in the art.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a method and a device for controlling synchronous nitrification and denitrification of sewage; the method and the device for controlling the synchronous nitrification and denitrification of the sewage based on the combined control of DO and the electronic transfer activity can monitor the macroscopic state in the reaction tank through the DO and the microscopic state of microorganisms through the electronic transfer activity, and can timely adjust the state in the reaction tank.
In order to achieve the above objects and other related objects, the present invention provides a synchronous nitrification and denitrification control device for sewage, which comprises a water inlet regulating tank, a synchronous nitrification and denitrification reaction tank and a secondary sedimentation tank; an aeration system, a submersible stirring system and DO on-line monitoring are arranged in the synchronous nitrification and denitrification reaction tank, and the synchronous nitrification and denitrification reaction tank further comprises an off-line electronic transfer activity rapid detection system and a variable frequency fan; the aeration system is connected with the variable frequency fan and is used for adjusting the aeration amount; the secondary sedimentation tank is provided with a sludge reflux pump; and the sludge reflux pump pumps the activated sludge back to the synchronous nitrification and denitrification reaction tank.
Further, sewage enters the regulating reservoir to be homogenized and metered, then flows automatically or is pumped into the synchronous nitrification and denitrification reaction tank, the aeration system in the tank is aerated by the air provided by the variable frequency fan to finish ammonia nitrogen nitrification, the submersible stirring system is also in a normally open state at the same time to ensure that sludge and sewage in the tank are in a complete mixing state, the air quantity of the variable frequency fan is controlled by data obtained by DO online monitoring and offline electronic transfer activity rapid detection to finish denitrification of the sewage, and therefore the aim of denitrification is achieved. The treated sewage enters a secondary sedimentation tank to be separated into sludge and water, and the sludge returns to the synchronous nitrification and denitrification reaction tank through a sludge reflux pump to maintain the concentration of the sludge in the tank; the excess sludge is also periodically discharged.
In order to achieve the above objects and other related objects, the present invention further provides a synchronous nitrification and denitrification control method for sewage, comprising the following steps:
i, rapid detection of electron transfer activity: the method comprises the following steps of pre-filling 1mL of Tris-HCl buffer solution with the concentration of 0.05mol/L and the pH value of 8.0, 1mL of 2,3, 5-triphenyltetrazolium chloride-sodium carboxymethyl cellulose solution and 0.5mL of 0.08mol/L sodium azide solution in a 20mL covered brown test tube; then taking 1.5ml of the muddy water mixed solution from the reaction tank, adding the muddy water mixed solution into a brown test tube with a cover, sealing the cover, uniformly mixing, and placing the mixture in a water bath at the temperature of 30 +/-0.5 ℃ for reaction for 30 min; adding 12ml of acetone, mixing uniformly, placing in a water bath with the temperature of 30 +/-0.5 ℃ for continuously reacting for 30min, and oscillating the covered brown test tube for 10s every 5 min; centrifuging 10ml of the mixed solution at 5000r/min for 3min, and measuring absorbance of the supernatant at 485nm in a spectrophotometer; simultaneously measuring the turbidity of the muddy water mixed solution; the absorbance/turbidity is taken as an electron transfer activity index;
II, domestication stage: inoculating activated sludge into a synchronous nitrification and denitrification reaction tank, and enabling the final sludge concentration in the synchronous nitrification and denitrification reaction tank to be more than or equal to 2500 mg/L; supplementing a carbon source to ensure that the C/N of the sewage in the synchronous nitrification and denitrification reaction tank is more than or equal to 5, starting a variable frequency fan for aeration, maintaining the DO in the synchronous nitrification and denitrification reaction tank to be monitored to be 1.0 +/-0.2 mg/L on line, and starting a submersible stirring system to start domestication and culture; measuring the absorbance/turbidity value at the moment as an initial value of the electron transfer activity; gradually nitrifying ammonia nitrogen into nitrate nitrogen along with the domestication, increasing DO, reducing aeration quantity to maintain the DO in the pool to be 0.6 +/-0.2 mg/L in online monitoring when the absorbance/turbidity value is 2-3 times of the initial value, continuing the domestication, starting the denitrification, and considering that the system domestication is successful when the absorbance/turbidity value is 6-8 times of the initial value, the ammonia nitrogen removal rate of the system reaches more than 90%, and the total nitrogen removal rate reaches more than 80%;
III, operation stage: after the system is successfully domesticated, appropriately supplementing a carbon source according to the C/N of the sewage to enable the C/N to be larger than or equal to 4, continuously feeding water, and jointly controlling the aeration quantity of a variable frequency fan by using an online DO monitoring value and the electron transfer activity, namely controlling the DO to be 0.6 +/-0.2 mg/L and the absorbance/turbidity value to be more than 6 times of an initial value;
when the absorbance/turbidity value is reduced by more than 20% for two consecutive days due to water quality fluctuation or external environment change, the nitrate concentration exceeds the standard, on the basis of controlling the quality of inlet water and stabilizing pH indexes, the aeration quantity of a variable frequency fan is reduced, the DO is reduced to be below 0.4mg/L, meanwhile, a carbon source is supplemented, the denitrification efficiency is improved until the absorbance/turbidity value returns to the normal level, and finally, the aeration quantity is restored to control the DO to be 0.6 +/-0.2 mg/L;
when the ammonia nitrogen concentration exceeds the standard and the absorbance/turbidity value is stable due to water quality fluctuation or external environment change, on the basis of controlling the indexes such as inlet water quality, pH and the like to be stable, the aeration quantity of the variable frequency fan is increased, so that the DO is increased to be more than 1.2mg/L, the fluctuation range of the absorbance/turbidity value is within 20 percent, the nitrification efficiency is improved, and finally, the aeration quantity is restored to control the DO to be 0.6 +/-0.2 mg/L;
after the sludge-water mixed liquid overflowing from the synchronous nitrification and denitrification reaction tank to the secondary sedimentation tank is subjected to sludge-water separation, the activated sludge is pumped back to the synchronous nitrification and denitrification reaction tank by the sludge reflux pump, so that the concentration of the sludge in the tank is ensured.
Further, the 2,3, 5-triphenyltetrazolium chloride-sodium carboxymethyl cellulose solution is a TTC-CMCNa solution, wherein the TTC concentration is 0.03mol/L, and the CMCNa concentration is 5 g/L.
Further, the external environmental change is manifested as an increase or decrease in the ambient temperature.
Wherein, the DO (dissolved oxygen) test method strictly complies with the national standard test method for wastewater quality analysis, or a high-precision dissolved oxygen analyzer is adopted.
Due to the application of the technical scheme, compared with the prior art, the invention has the beneficial effects that:
(1) according to the invention, the macroscopic dissolved oxygen state of the sewage in the reaction tank is monitored on line by DO, and the microscopic activity of microorganisms in the reaction tank is monitored by measuring the electron transfer activity (absorbance/turbidity) off line; the aeration quantity of the fan is controlled by combining the data of the two to ensure the synchronous completion of the nitrification and denitrification;
(2) the invention adopts a pre-loaded composite reagent for detecting the electron transfer activity, wherein a TTC reagent is an electron acceptor and can accept electrons in an electron transfer chain of a microorganism and develop color; sodium azide blocks cytochrome in the last step in an electron transfer chain to transfer electrons to oxygen, so that TTC (time to temperature) can develop color to reflect the efficiency of the whole electron transfer chain, the whole reaction can be carried out in an aerobic environment, and the deoxidation operation is avoided; CMCNa can make the sludge in a suspended state, combine the substrate and the microbial cell components and completely react. The whole process can be completed only in 1.5h, the operation is simple, and the data is reliable.
(3) When the synchronous nitrification and denitrification of the sewage in the control tank are carried out, the values of the electron transfer activity (absorbance/turbidity) in each reaction tank are different due to different sewage qualities and different sewage treatment processes, so that the ratio and the variation amplitude of the values are taken as control indexes, the nitrification and denitrification activity of microorganisms in each sewage treatment engineering tank can be effectively reflected, the control system is closer to the actual engineering, and the control system is also beneficial to the actual regulation and control operation on site.
Drawings
FIG. 1 is a schematic structural diagram of a synchronous nitrification and denitrification control device for sewage.
In the figure: 1. a water inlet adjusting tank; 2. a synchronous nitrification and denitrification reaction tank; 3. a secondary sedimentation tank; 4. an aeration system; 5. a submersible mixing system; 6. DO online monitoring; 7. off-line rapid detection of electron transfer activity; 8. a variable frequency fan; 9. a sludge reflux pump.
Detailed Description
Other advantages and capabilities of the present invention will be readily apparent to those skilled in the art from the present disclosure by describing in detail embodiments of the present invention with reference to the accompanying drawings.
Example (b):
as shown in fig. 1, a synchronous nitrification and denitrification control device for sewage comprises a water inlet adjusting tank 1, a synchronous nitrification and denitrification reaction tank 2 and a secondary sedimentation tank 3; an aeration system 4, a submersible stirring system 5 and a DO online monitoring 6 are arranged in the synchronous nitrification and denitrification reaction tank 2, and the synchronous nitrification and denitrification reaction tank also comprises an offline electronic transfer activity rapid detection system 7 and a variable frequency fan 8; the aeration system 4 is connected with the variable frequency fan 8 and is used for adjusting aeration quantity; the secondary sedimentation tank 3 is provided with a sludge reflux pump 9; and the sludge reflux pump 9 pumps the activated sludge back to the synchronous nitrification and denitrification reaction tank 2.
Sewage gets into equalizing basin 1 and carries out the homogeneity and measure equally, then flow automatically or pump into synchronous nitrification-denitrification reaction pond 2, aeration system 4 provides the air by variable frequency fan 8 in the pond and carries out the aeration, accomplish the ammonia nitrogen and nitrify, dive mixing system 5 also is in the normal open state simultaneously, guarantee that mud and sewage are in the complete mixing state in the pond, through the DO on-line monitoring 6 and the data control variable frequency fan 8 amount of wind that the active short-term test 7 of off-line electron transfer obtained, accomplish the sewage denitrification, thereby reach the denitrogenation purpose. The treated sewage enters a secondary sedimentation tank 3 to complete sludge-water separation, the sludge returns to the synchronous nitrification and denitrification reaction tank 2 through a sludge reflux pump 9 to maintain the concentration of the sludge in the tank, and the residual sludge is discharged periodically.
Synchronous sewage nitrification and denitrification control method
The wastewater produced by a certain butyronitrile glove factory is used as raw water, and various indexes of the inlet water are as follows: COD Cr ＝250～400mg/L，BOD 5 80-125 mg/L, 2-4 mg/L ammonia nitrogen, 20-30mg/L Kjeldahl nitrogen, 250-380 mg/L total nitrogen, 1-3 mg/L TP, and 7.2-8.5 pH. The test system is shown in figure 1, the reactor is made of organic glass, and the effective volume of the synchronous nitrification-denitrification reaction tank is 500L.
The specific operation is as follows:
i, rapid detection of electron transfer activity: a20 mL covered brown tube is preloaded with 1mL of Tris-HCl buffer solution (0.05mol/L, pH8.0), 1mL of TTC-CMCNa solution (2,3, 5-triphenyltetrazolium chloride-sodium carboxymethyl cellulose, TTC concentration of 0.03mol/L, CMCNa concentration of 5g/L) and 0.5mL of sodium azide solution (0.08 mol/L). Taking 1.5ml of muddy water mixed solution from the reaction tank, adding the muddy water mixed solution into the test tube, sealing the test tube, uniformly mixing, and placing the test tube in a water bath (30 +/-0.5 ℃) for reacting for 30 min; adding 12ml of acetone, mixing uniformly, placing in a water bath (30 +/-0.5 ℃) to continue reacting for 30min, and shaking the test tube for 10s every 5 min; centrifuging 10ml of the mixture (5000r/min,3min), and measuring absorbance of the supernatant at 485nm in a spectrophotometer. And simultaneously measuring the turbidity of the muddy water mixed solution. The absorbance/turbidity was used as an index of the electron transfer activity. During normal operation, the index is measured once a day; when the fluctuation amplitude of the sample exceeds 20%, the measurement is carried out twice a day.
II, domestication stage: inoculating activated sludge taken from other sewage treatment plants into a synchronous nitrification and denitrification reaction tank, enabling the final concentration of the sludge in the tank to be not less than 2500mg/L, supplementing carbon sources (conventional carbon sources of the sewage treatment plants, glucose, methanol or sodium acetate and the like), enabling C/N in sewage to be not less than 5, starting a submersible mixer, starting a variable frequency fan for aeration, maintaining DO in the tank to be 1.0 +/-0.2 mg/L, and starting acclimatization and culture. Immediately after the inoculation of the activated sludge, the absorbance and turbidity were measured according to the method in step I, and the absorbance/turbidity value at this time was used as an initial value of the electron transfer activity. Gradually nitrifying ammonia nitrogen into nitrate nitrogen along with the domestication, increasing DO, adjusting a fan to reduce aeration quantity when the absorbance/turbidity value is 2-3 times of an initial value so as to reduce the DO in the pond to a range of 0.6 +/-0.2 mg/L, continuing the domestication, starting the denitrification, and when the absorbance/turbidity value is 6-8 times of the initial value, ensuring that the ammonia nitrogen removal rate of the system reaches more than 90 percent and the total nitrogen removal rate reaches more than 80 percent, namely considering that the system domestication is successful. The system starting time is 40-70 days.
III, operating stage: after the system acclimatization is successful, because the C/N in the inlet water is seriously imbalanced (close to 1:1), a carbon source (a conventional carbon source of a sewage treatment plant, glucose, methanol or sodium acetate and the like) is appropriately supplemented to ensure that the C/N is not lower than 4, water is continuously fed, and the aeration quantity of a fan is controlled by combining a DO online monitoring value and the electron transfer activity, namely the DO is controlled to be 0.6 +/-0.2 mg/L, and the absorbance/turbidity value is more than 6 times of the initial value. The ammonia nitrogen removal rate of the system is stabilized to be more than 90 percent, and the total nitrogen removal rate is stabilized to be more than 80 percent.
When the water in the washing tank is replaced regularly in a workshop, the concentration of the nitrate in the inlet water exceeds 1 time, the absorbance/turbidity value is immediately reduced, the reduction amplitude reaches 40%, the pH of the inlet water is maintained at 7-8, the amount of the inlet water is adjusted to 70% of the normal value, the aeration quantity of a fan is reduced, the DO is adjusted to 0.4mg/L, meanwhile, a carbon source is supplemented, the C/N reaches 6, the denitrification efficiency is improved until the absorbance/turbidity value returns to the normal level, the total nitrogen removal rate returns to more than 80%, then the inlet water flow is restored to the normal value, and the aeration quantity is further improved to restore the DO to 0.6 +/-0.2 mg/L. The whole adjusting process needs 2-5 days.
When the system runs in winter, the temperature is reduced, nitrobacteria are greatly influenced by the temperature, the efficiency is reduced, the ammonia nitrogen concentration exceeds the standard, the absorbance/turbidity value is basically stable, the water temperature in the reaction tank is maintained to be not lower than 15 ℃ on the basis of controlling the stability of indexes such as the quality of inlet water, the pH value and the like, the aeration quantity of a fan is increased, the DO is increased to be more than 1.2mg/L, at the moment, the absorbance/turbidity value is slightly increased, the fluctuation range is within 20%, the ammonia nitrogen removal rate of the system is increased to be more than 90% after the system runs for a period of time, and the total nitrogen removal rate is also recovered to be more than 80%. Finally, the aeration rate is recovered to control DO to be 0.6 +/-0.2 mg/L. The whole adjusting process needs 4-10 days.
Water intake COD during the 12 month test period Cr The average concentration of ammonia nitrogen, Kjeldahl nitrogen and total nitrogen is 308.2mg/L and 2.6mg/L respectively25.9mg/L, 342.0mg/L, COD of the effluent Cr The average concentrations of ammonia nitrogen, Kjeldahl nitrogen and total nitrogen are respectively 78.4mg/L, 0.1mg/L, 3.2mg/L and 30.7mg/L, and the average removal rates are 74.6%, 96.2%, 87.6% and 91%.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (3)
1. A synchronous sewage nitrification and denitrification control method is characterized in that a synchronous sewage nitrification and denitrification control device used in the method comprises a water inlet adjusting tank (1), a synchronous nitrification and denitrification reaction tank (2) and a secondary sedimentation tank (3); an aeration system (4), a submersible stirring system (5) and DO on-line monitoring (6), an off-line electronic transfer activity rapid detection system (7) and a variable frequency fan (8) are arranged in the synchronous nitrification and denitrification reaction tank (2); the aeration system (4) is connected with the variable frequency fan (8) and is used for adjusting aeration quantity; the secondary sedimentation tank (3) is provided with a sludge reflux pump (9); the sludge reflux pump (9) pumps the activated sludge back to the synchronous nitrification and denitrification reaction tank (2); the method is characterized in that: the method comprises the following steps:
i, rapid detection of electron transfer activity: the method comprises the following steps of pre-filling 1mL of Tris-HCl buffer solution with the concentration of 0.05mol/L and the pH value of 8.0, 1mL of 2,3, 5-triphenyltetrazolium chloride-sodium carboxymethyl cellulose solution and 0.5mL of 0.08mol/L sodium azide solution in a 20mL covered brown test tube; then taking 1.5ml of muddy water mixed solution from the reaction tank, adding the muddy water mixed solution into a brown test tube with a cover, sealing the cover, uniformly mixing, and placing the mixture in a water bath at the temperature of 30 +/-0.5 ℃ for reaction for 30 min; adding 12ml of acetone, mixing uniformly, placing in a water bath with the temperature of 30 +/-0.5 ℃ for continuously reacting for 30min, and shaking the covered brown test tube for 10s every 5 min; centrifuging 10ml of the mixed solution at 5000r/min for 3min, and measuring absorbance of the supernatant at 485nm in a spectrophotometer; simultaneously measuring the turbidity of the muddy water mixed solution; the ratio of absorbance/turbidity is used as an electron transfer activity index;
II, domestication stage: inoculating activated sludge into a synchronous nitrification and denitrification reaction tank, and enabling the final sludge concentration in the synchronous nitrification and denitrification reaction tank to be more than or equal to 2500 mg/L; supplementing a carbon source to ensure that the C/N of the sewage in the synchronous nitrification and denitrification reaction tank is more than or equal to 5, starting a variable frequency fan for aeration, maintaining the DO in the synchronous nitrification and denitrification reaction tank to be monitored to be 1.0 +/-0.2 mg/L on line, and starting a submersible stirring system to start domestication and culture; measuring the absorbance/turbidity value at the moment as an initial value of the electron transfer activity; gradually nitrifying ammonia nitrogen into nitrate nitrogen along with the domestication, increasing DO, reducing aeration quantity to maintain the DO in the pool to be 0.6 +/-0.2 mg/L in online monitoring when the absorbance/turbidity value is 2-3 times of an initial value, continuing the domestication, starting the denitrification, and considering that the system domestication is successful when the absorbance/turbidity value is 6-8 times of the initial value, the ammonia nitrogen removal rate of the system reaches more than 90%, and the total nitrogen removal rate reaches more than 80%;
III, operation stage: after the system is successfully domesticated, appropriately supplementing a carbon source according to the C/N of the sewage to enable the C/N to be larger than or equal to 4, continuously feeding water, and jointly controlling the aeration quantity of a variable frequency fan by using an online DO monitoring value and the electron transfer activity, namely controlling the DO to be 0.6 +/-0.2 mg/L and the absorbance/turbidity value to be more than 6 times of an initial value; when the absorbance/turbidity value is reduced by more than 20 percent for two consecutive days due to water quality fluctuation or increase or decrease of the environmental temperature, the nitrate concentration exceeds the standard, the aeration quantity of a variable frequency fan is reduced on the basis of controlling the stability of the water quality of the inlet water, the DO is reduced to be below 0.4mg/L, a carbon source is supplemented, the denitrification efficiency is improved until the absorbance/turbidity value returns to the normal level, and finally the aeration quantity is restored to control the DO to be 0.6 +/-0.2 mg/L; when the ammonia nitrogen concentration exceeds the standard and the absorbance/turbidity value is stable due to water quality fluctuation or increase or decrease of the environmental temperature, on the basis of controlling the stability of the water quality of inlet water, the aeration quantity of the variable frequency fan is increased, so that the DO is increased to be more than 1.2mg/L, meanwhile, the fluctuation range of the absorbance/turbidity value is within 20 percent, the nitrification efficiency is improved, and finally, the aeration quantity is restored to control the DO to be 0.6 +/-0.2 mg/L; after the sludge-water mixed liquid overflowing from the synchronous nitrification and denitrification reaction tank to the secondary sedimentation tank is subjected to sludge-water separation, the activated sludge is pumped back to the synchronous nitrification and denitrification reaction tank by the sludge reflux pump, so that the concentration of the sludge in the tank is ensured.
2. The synchronous sewage nitrification and denitrification control method according to claim 1, characterized in that: the 2,3, 5-triphenyltetrazolium chloride-sodium carboxymethyl cellulose solution is a TTC-CMCNa solution, wherein the TTC concentration is 0.03mol/L, and the CMCNa concentration is 5 g/L.
3. The synchronous sewage nitrification and denitrification control method according to claim 1, characterized in that: the submersible stirring system (5) is in a normally open state.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1730133A1 (en) * | 1990-05-24 | 1992-04-30 | Харьковский Отдел Всесоюзного Комплексного Научно-Исследовательского И Конструкторско-Технологического Института Водоснабжения, Канализации, Гидротехнических Сооружений И Инженерной Гидрогеологии "Водгео" | Method for determining quantity of nitrifying bacteria in investigated material |
| CN1982878A (en) * | 2005-12-16 | 2007-06-20 | 尹军 | Method for diagnosing active mud-ratio electronic transferring system |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2302281A1 (en) * | 1975-02-28 | 1976-09-24 | Anvar | Measuring the activity of activated sludge in water treatment plant - by reacting with triphenyl tetrazolium chloride to form red colour |
| CN100503470C (en) * | 2006-11-14 | 2009-06-24 | 北京工业大学 | Control method and device of A2/O oxidation trench technology synchronous nitration and denitrification |
| CN101251473B (en) * | 2008-03-28 | 2013-02-13 | 上海市政工程设计研究总院 | Method for testing microbial activity of artificial wet land sewage treatment system |
| CN100592076C (en) * | 2008-04-15 | 2010-02-24 | 河海大学 | Preprocessing method for measuring desaturase activity of drinking water biological active carbon treatment process |
| CN107365830A (en) * | 2016-05-11 | 2017-11-21 | 杭州健昵福生物科技有限公司 | A kind of oxidoreductase activity method of testing |
| CN107870153A (en) * | 2017-11-02 | 2018-04-03 | 南京工业大学 | Method for detecting influence of phenolic compound on nitrobacteria electron transfer system |
| CN109574421A (en) * | 2019-01-31 | 2019-04-05 | 清华大学深圳研究生院 | A kind of reverse osmosis concentration enhanced water processing method and equipment |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1730133A1 (en) * | 1990-05-24 | 1992-04-30 | Харьковский Отдел Всесоюзного Комплексного Научно-Исследовательского И Конструкторско-Технологического Института Водоснабжения, Канализации, Гидротехнических Сооружений И Инженерной Гидрогеологии "Водгео" | Method for determining quantity of nitrifying bacteria in investigated material |
| CN1982878A (en) * | 2005-12-16 | 2007-06-20 | 尹军 | Method for diagnosing active mud-ratio electronic transferring system |
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