CN111595654A - Device and assembly for collecting biochemical samples - Google Patents
Device and assembly for collecting biochemical samples Download PDFInfo
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- CN111595654A CN111595654A CN202010517409.6A CN202010517409A CN111595654A CN 111595654 A CN111595654 A CN 111595654A CN 202010517409 A CN202010517409 A CN 202010517409A CN 111595654 A CN111595654 A CN 111595654A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Abstract
The invention discloses a device and a component for collecting biochemical samples, belonging to the technical field of biochemical equipment and comprising a collecting and filtering mechanism, a separation and purification mechanism and a driving mechanism; the driving mechanism is provided with a collecting and filtering mechanism and a separating and purifying mechanism in a removable way; a through drainage tube is horizontally connected between the collecting and filtering mechanism and the separating and purifying mechanism; the shunt mechanism of the separation and purification mechanism comprises a shunt pipe adopting the Venturi tube principle and resistor loops connected in series with different resistance values; the driving mechanism can drive the first connecting shaft and the second connecting shaft to rotate in a butt joint mode, and the first electrode and the second electrode are connected to conduct electrophoresis separation. The device solves the problems of mixing of separated sample belts, sample denaturation and easy blockage of capillaries caused by medium temperature rise due to heating in the electrophoretic separation of biochemical samples, reduces pollution by recycling buffer solution, has high separation efficiency and good effect, can ensure the detection accuracy by preserving the samples in vacuum, and can separate and collect all the required types of separated samples at one time.
Description
Technical Field
The invention belongs to the technical field of biochemical equipment, and particularly relates to a device and a component for collecting biochemical samples.
Background
The collection of biochemical samples as a clinical activity to assist in disease diagnosis and treatment is of great importance in clinical diagnostic applications, where clinical samples include blood, stool, urine, pleural effusion, ascites, and the like. The differential counting of sample components, particularly the rapid and accurate quantitative counting of certain rare components, is important for the physician to properly diagnose the patient's condition and to monitor the patient's recovery process.
Patent US08062903B2 provides a method and apparatus for overcoming deleterious diffusion effects in a sample liquid stream by forming segmented liquids from the sample liquid stream in an immiscible liquid stream. The liquid is formed at the intersection of a channel providing a flow of sample liquid and a channel providing a flow of immiscible liquid. The formed liquid separates the portions from which the liquid forms the liquid stream, thereby minimizing the adverse effects of diffusion that occur in a continuous liquid stream. However, in the prior art, liquid separation is easy to block in a capillary, the electrophoretic separation speed is slow, most of work done by current is converted into heat, and the temperature of a medium is easy to rise to cause mixing of separated sample bands and sample denaturation. The invention provides a novel biochemical sample collection device for solving the problems, which can provide a high-quality and stable separated sample and improve the detection efficiency and accuracy.
Disclosure of Invention
The invention aims to provide a device and a component for collecting biochemical samples, which solve the problems of mixing of separated sample belts and sample denaturation caused by medium temperature rise due to heat generation during the electrophoretic separation of the biochemical samples; the phenomenon that the capillary tube is easy to block is solved; meanwhile, the separation efficiency and effect are increased, the sample is preserved in vacuum, the detection accuracy is guaranteed, the buffer solution can be recycled, the pollution is reduced, and all required types of separation samples can be separated and collected at one time.
The technical scheme adopted by the invention for realizing the purpose is as follows: the device for collecting the biochemical samples comprises a collecting and filtering mechanism and a separating and purifying mechanism, wherein the collecting and filtering mechanism comprises a first cavity, the height of the first cavity is adjustable, an opening is formed in the first cavity, and a first electrode capable of being electrified is arranged in the first cavity; the original sample collecting bottle is used for accommodating a biochemical sample to be processed and is detachably connected to one opening side of the cavity; the first rotating mechanism comprises a first connecting shaft with two ends respectively connected with the first notch and the screw mechanism and is used for driving the original sample collecting bottle to move up and down along a first cavity, and the first cavity is divided into two areas by a filter membrane connected with the middle part of the first connecting shaft; the separation and purification mechanism comprises a second cavity, a buffer solution and a second electrode, wherein the buffer solution can be transferred into the first cavity, and the second electrode has the polarity opposite to that of the first electrode; the second rotating mechanism comprises a second connecting shaft of which two ends are respectively connected with the second notch and the rotary table, and the rotary table is provided with at least one removable separated sample collecting bottle; the shunting mechanism is used for separating and discharging the processed biochemical sample and the buffer solution; the docking mechanism can adaptively adjust the inclination angle to dock and fill the processed biochemical sample into the separated sample collecting bottle.
The lower part of the cavity of the collecting and filtering mechanism is detachably connected with an original sample collecting bottle for collecting a biochemical sample to be treated; a buffer solution which can be transferred into the first cavity is filled in the second cavity of the separation and purification mechanism; a through drainage tube is horizontally connected between the first cavity and the second cavity side wall corresponding to the separated sample inflow port and the return tube of the cross tube. Twist into cavity one opening side behind former original sample collection bottle collection sample and form the confined space, when rotary mechanism rotates, under the guide arm guiding action, thereby the connecting axle drives screw rod motion and drives former original sample collection bottle up-and-down motion, utilize the suction principle to shift the buffer solution in the cavity two to the cavity one in, sample in the former original sample collection bottle is stirred by screw mechanism in the up-and-down motion in-process simultaneously, former original sample collection bottle stops at the upper limit position after the stirring is accomplished, precipitate and mixed liquid are separated by the diaphragm, the stirring is filtered the action and can be decomposed the mixture with higher speed, separation efficiency is improved, and be applicable to different biochemical samples. And conducting electrophoresis separation on the mixed liquid of the first electrode and the second electrode, separating the components due to differential migration, changing the motion direction of the components through a resistance loop in the shunting mechanism, enabling the components to continuously move downwards and be ejected out through the Venturi tube shunt pipe to form a negative pressure area. In the process that the docking mechanism docks the corresponding separated sample collecting bottle, the shunt tube and the negative pressure generated continuously pump the processed biochemical sample into the separated sample collecting bottle, so that the cross capillary is prevented from being blocked; the separation efficiency is accelerated, and the electrophoresis time is reduced, so that the damage of heating to the quality of a separated sample is reduced; the collected sample in the sample collecting bottle is separated into homogeneous flow in the process of spraying the shunt pipe, and the homogeneous flow is directly used for good detection effect; and the separated sample collecting bottle is simultaneously provided with vacuum, so that bacterial infection can be reduced, the detection performance of the separated sample can be ensured, the detector is used for detecting a sample component separation belt, different separated sample collecting bottles are driven to rotate to butt joint the abutting mechanism to collect different separated samples under the rotation of the second rotating mechanism, and all separated samples of required types can be separated and collected at one time.
Preferably, the first cavity comprises an upper shell with an opening at one end and an annular butt-joint ring, and the upper shell and the annular butt-joint ring are connected by a telescopic elastomer; the outer wall of the upper shell is connected with an L-shaped guide rod.
The middle of the first cavity body contains a telescopic elastic body, so that the original sample collecting bottle moves up and down to place the spiral mechanism in the first cavity body to stir a biochemical sample to be processed, and the disassembly, collection, cleaning and installation are not influenced; the L-shaped guide rod connected with the outer wall of the upper shell is used for guiding and stopping rotation in cooperation with the original sample collecting bottle and only moves up and down linearly.
Preferably, the sample collection bottle is a shell with an opening at one end, and the opening side is in threaded connection with the butt ring; the outer wall is provided with a guide groove matched with the guide rod along the axial direction; the center of the inner cavity is connected with a screw rod connected with a thread pair of the connecting shaft.
The original sample collecting bottle is detachably connected with the butt-joint ring, so that a biochemical sample to be treated can be collected conveniently; the guide groove is matched with the guide rod to guide and stop rotation, and only moves up and down linearly under the drive of the first connecting shaft and the screw rod.
Preferably, the first cavity and the first rotating mechanism are coaxial, and the contact surface is sealed; the second cavity and the second rotating mechanism are coaxial, and the contact surface is sealed. The coaxial arrangement has uniform stress and simple and reliable structure.
Preferably, the separation sample collecting bottle comprises a tubular bottle body and a connecting part arranged at the opening part of the bottle body, and the processed biochemical sample is limited in the forming area of the tubular bottle body and the connecting part; the connecting part is detachably connected with the sealing cover through threads, and a seal is sealed on the connecting part close to the sealing cover; the seal is made of elastic material with middle cut and automatic recovery seal.
The separated sample collecting bottle comprises a tubular bottle body and a threaded connecting part for containing the processed biochemical sample, and the connecting part comprises an elastic seal with a middle cut and capable of automatically returning to be closed, so that internal vacuum loss generated by the shunting mechanism is avoided; can dismantle simultaneously to be connected with the closing cap and protect and prevent that the mistake from touching to open and seal the influence separation sample quality.
Preferably, the shunt mechanism comprises a cross pipe and a resistance loop arranged in the cross pipe, the cross pipe is a cross capillary, the closed end of the opposite side of the separated sample inflow port of the cross pipe is provided with a detector, and the other two ends of the cross pipe respectively correspond to the outlet of the buffer solution and the installation port of the shunt pipe; an L-shaped return pipe with a downward pipe orifice is arranged above the flow dividing mechanism and is connected with the two inner side walls of the cavity.
The cross pipe is a cross capillary and is used for conveying different separation components after electrophoretic separation, a detector for detecting a separation band of the sample components is arranged at the closed end on the opposite side of the separation sample inflow port, the sample components are accumulated under the action of a resistance loop, and the detection is sensitive; the other two ends respectively correspond to the buffer solution outlet and the shunt tube mounting port and are used for discharging the separated sample; the flow dividing mechanism and the L-shaped return pipe which are connected with the inner side wall of the cavity II can complete the cyclic utilization of the buffer solution in the cavity I and the cavity II and reduce the pollution.
Preferably, the shunt pipe is a Venturi pipe, a one-way valve is arranged in the Venturi pipe, and the other end of the Venturi pipe is connected with a hose; the resistance loop comprises a first resistance positioned at the outlet side of the buffer solution and a second resistance close to the shunt tube, which jointly form the resistance loop; the first resistor is larger than the second resistor.
The shunt pipe is a Venturi pipe, the first resistance of the resistance loop is larger than the second resistance, the moving direction of the separated sample is changed under the action of the resistance loop, the separated sample continuously moves downwards and is jetted out through the shunt pipe of the Venturi pipe to form a negative pressure area, and the one-way valve is used for controlling whether the separated sample flows out or not.
Preferably, the docking mechanism comprises a fixed seat positioned on the bottom surface of the cavity and a tubular sleeve hinged with the fixed seat; a spring elastic part is connected between the outer wall of the sleeve and the two bottom surfaces of the cavity; the elastic piece can adaptively adjust the inclination angle between the sleeve and the second bottom surface of the cavity; the hose penetrates through the bottom of the cavity II and the sleeve and is rotatably inserted into the seal of the corresponding separated sample collection bottle by the sleeve for drainage.
Docking mechanism can insert the seal that corresponds the bottleneck under the elastic component effect and collect the separation sample, when the detector detected that the completion collected this type of separation sample, when rotating and switching this separation sample collecting bottle, separation sample collecting bottle promoted docking mechanism tilt up, and the compression elastic component rotated to target in place until another separation sample collecting bottle, and docking mechanism reinserts under the elastic component effect and seals in corresponding bottleneck and carry out the injection collection.
An assembly for biochemical sample collection, utilizing the device for biochemical sample collection described above, further comprising a drive mechanism; the driving mechanism is removably provided with a collecting and filtering mechanism and a separating and purifying mechanism which are arranged by magnetic adsorption or threads; a through drainage tube is horizontally connected between the first cavity and the second cavity side wall corresponding to the separation sample inflow port and the return tube of the cross tube; the driving mechanism can drive the first connecting shaft and the second connecting shaft to rotate respectively in the butt joint of the first notch and the second notch; the driving mechanism can connect and electrify the first electrode and the second electrode to carry out electrophoretic separation.
A subassembly for biochemical sample is collected, including collecting filtering mechanism, separation and purification mechanism and actuating mechanism, collect filtering mechanism and separation and purification mechanism and adopt magnetic force to adsorb or the screw thread is installed the replacement of being convenient for on actuating mechanism, and actuating mechanism is reuse mechanism, helps reduce cost. A through drainage tube is horizontally connected between the first cavity and the second cavity and controls whether the first electrode and the second electrode are electrified or not to carry out electrophoretic separation on a sample; and provides power source for the first connecting shaft and the second connecting shaft.
Preferably, the driving mechanism comprises a shell, a driver, a first motor and a second motor, wherein the driver, the first motor and the second motor are arranged on the bottom surface of an inner cavity of the shell in parallel; the first fork piece and the second fork piece are in limit fit with the first notch and the second notch in two directions and are in butt joint driving.
And after the first fork piece and the second fork piece of the driving mechanism are in butt joint with the first notch and the second notch, the driving mechanism is controlled and driven by the first motor and the second motor.
Compared with the prior art, the invention has the beneficial effects that: the collection and filtration mechanism, the separation and purification mechanism and the driving mechanism are combined, and the stirring, the filtration, the separation, the collection and the storage of different types of biochemical samples are integrated. The collecting and filtering mechanism adopts a pressure difference principle to complete the transfer of buffer solution in the first cavity and the second cavity, meanwhile, samples to be processed in the original sample collecting bottle are stirred by the spiral mechanism in the vertical movement process to be separated and mixed in an accelerating way and are separated into precipitates and mixed liquid by the diaphragm, and the separation efficiency can be effectively improved by combining the separation and purification mechanism to carry out electrophoretic separation on the mixed liquid which is filtered in advance, so that the separating and filtering mechanism is suitable for the separation of different biochemical samples. The separation and purification mechanism adopts the Venturi tube principle and is combined with a resistance loop, all components are separated due to electrophoresis differential migration, the movement direction of the components is changed through the resistance loops with different resistance values, the components are enabled to continuously move towards the direction with small resistance value and are sprayed out through a Venturi tube shunt tube to form a negative pressure area, a closed space is formed by combining a separation sample collecting bottle butted with the butting mechanism, the treated biochemical sample is sprayed into the separation sample collecting bottle through the shunt tube, negative pressure is continuously formed in the bottle while the separation sample collecting bottle is sprayed out, the suction efficiency is increased, the cross capillary is prevented from being blocked, the separation efficiency is accelerated, the electrophoresis time is shortened, and the separation sample belt mixing and the separation sample quality damage; the collected sample in the sample collecting bottle is separated into homogeneous flow in the process of spraying the shunt pipe, and the homogeneous flow can be directly used for detection and has good effect; after the docking mechanism is withdrawn, the separated sample collecting bottles are automatically sealed, the vacuum is reserved inside the separated sample collecting bottles, bacterial infection can be reduced, the detection accuracy of the separated samples is guaranteed, the detector is used for detecting the component separation belts of the samples, different separated samples are collected by rotating the docking mechanism at different separated sample collecting bottles, and all the required separated samples can be separated and collected at one time. The device solves the problems of mixing of separated sample belts and sample denaturation caused by the rise of medium temperature due to heating in the process of electrophoretic separation of biochemical samples, solves the problem of easy blockage of capillaries, can recycle buffer solution to reduce pollution, has high separation efficiency and good separation effect, is favorable for ensuring the accuracy of detection by preserving samples in vacuum, and can separate and collect all required types of separated samples at one time.
Drawings
FIG. 1 is a schematic diagram of an initial structure of the present invention;
FIG. 2 is a schematic diagram of the separation process of the present invention;
FIG. 3 is a schematic view of the collection filter mechanism of the present invention;
FIG. 4 is a schematic structural diagram of a separation and purification mechanism according to the present invention;
FIG. 5 is a schematic structural view of a shunt mechanism of the present invention;
FIG. 6 is a schematic view of a separated sample collection bottle placement configuration according to an embodiment of the present invention;
FIG. 7 is a schematic structural diagram of a docking mechanism according to the present invention;
FIG. 8 is a schematic view of a detached sample collection vial of the present invention;
fig. 9 is a schematic structural diagram of the driving mechanism of the present invention.
Description of reference numerals: a collecting and filtering mechanism 1; a separation and purification mechanism 2; a drive mechanism 3; a first cavity 11; a first rotating mechanism 12; an as-received sample collection bottle 13; a first electrode 14; an upper case 111; a first cover 111 a; a second cover 111 b; an elastic body 112; a docking ring 113; a guide rod 114; a first notch 121; a first connecting shaft 122; a screw mechanism 123; a diaphragm 124; a guide groove 131; a screw 132; a second cavity 21; a second rotating mechanism 22; a return pipe 23; a flow dividing mechanism 24; a second electrode 25; a buffer solution 26; a separate sample collection vial 27; a docking mechanism 28; a third cover 21 a; a cover four 21 b; a second notch 221; a second connecting shaft 222; a turn table 223; the grooves 223 a; a cross 241; a detector 242; a shunt tube 243; the hose 243 a; a check valve 244; a resistance loop 245; a first resistor 245 a; a second resistor 245 b; a body 271; a connecting portion 272; a seal 273; a cover 274; a fixed seat 281; a sleeve 282; an elastic member 283; a housing 31; a first motor 32; a second motor 33; a driver 34; a first magnetic ring 35; a second magnetic ring 36; a first flange 311; a second flange 312; a first electrifying device 313; a second energizing device 314; a first fork element 321; and a second fork 331.
Detailed Description
The invention is described in further detail below with reference to examples and figures:
example 1:
as shown in fig. 1-9, the device for collecting biochemical samples comprises a collecting and filtering mechanism 1 and a separating and purifying mechanism 2, wherein the collecting and filtering mechanism 1 comprises a first cavity 11, the height of the cavity is adjustable, an opening is formed, and a first electrode 14 capable of being electrified is arranged in the cavity; an original sample collecting bottle 13 for accommodating a biochemical sample to be processed, detachably connected to the opening side of the first cavity 11; the rotating mechanism I12 comprises a connecting shaft I122 with two ends respectively connected with the notch I121 and the screw mechanism 123 and is used for driving the original sample collection bottle 13 to move up and down along the cavity I11, and the cavity I11 is divided into two areas by a filter membrane 124 connected with the middle part of the connecting shaft I122; the separation and purification mechanism 2 comprises a second cavity 21, a second electrode 25 and a buffer solution 26, wherein the buffer solution 26 can be transferred into the first cavity 11, and the polarity of the second electrode is opposite to that of the first electrode 14; the second rotating mechanism 22 comprises a second connecting shaft 222 with two ends respectively connected with the second notch 221 and the rotary table 223, six removable separated sample collecting bottles 27 are annularly placed on the rotary table 223, each bottle is respectively pushed into six concave grooves 223a of the annular array for limiting, and the grooves 223a are close to openings at the outer ring of the rotary table 223 and are matched with the inner ring surfaces of the separated sample collecting bottles 27; a flow dividing mechanism 24 for separating and discharging the processed biochemical sample and the buffer solution 26; the docking mechanism 28 is adapted to adjust the tilt angle to dock the processed biochemical sample into the separated sample collection bottle 27.
The lower part of a cavity I11 of the collecting and filtering mechanism 1 is detachably connected with an original sample collecting bottle 13 for collecting a biochemical sample to be treated; a second cavity 21 of the separation and purification mechanism 2 is filled with a buffer solution 26 which can be transferred into the first cavity 11; a through drainage tube is horizontally connected between the first cavity 11 and the side wall of the second cavity 21 corresponding to the separated sample inlet and the return tube 23 of the cross tube 241. The original sample collecting bottle 13 is screwed into the opening side of the cavity I11 to form a closed space after collecting a sample, when the rotating mechanism I12 rotates, under the guiding effect of the guide rod 114, the connecting shaft I122 drives the screw rod 132 to move so as to drive the original sample collecting bottle 13 to move up and down, the buffer solution 26 in the cavity II 21 is transferred into the cavity I11 by utilizing the suction principle, meanwhile, the sample in the original sample collecting bottle 13 is stirred by the spiral mechanism 123 in the up-and-down movement process, the original sample collecting bottle 13 stays at the upper limit position after the stirring is completed, precipitates and mixed liquid are separated by the diaphragm 124, and the stirring and filtering actions can be decomposed and mixed at high speed, so that the separation efficiency is improved, and the device is. The first conducting electrode 14 and the second conducting electrode 25 conduct electrophoresis separation on the mixed liquid, the components are separated due to differential migration, the movement direction of the components is changed through the resistance loop 245 in the shunt mechanism 24, the components are enabled to continuously move downwards, and the components are ejected out through the venturi pipe shunt 243 to form a negative pressure area. In the process of docking the corresponding separated sample collection bottle 27 by the docking mechanism 28, the shunt pipe 243 and the negative pressure generated constantly draw the processed biochemical sample into the separated sample collection bottle 27, so as to prevent the cross capillary from being blocked; the separation efficiency is accelerated, and the electrophoresis time is reduced, so that the damage of heating to the quality of a separated sample is reduced; the collected sample in the sample collecting bottle 27 is separated into homogeneous flow in the process of spraying the shunt pipe 243, and the homogeneous flow is directly used for good detection effect; and the separated sample collecting bottle 27 is simultaneously provided with vacuum, so that bacterial infection can be reduced, the detection performance of the separated sample can be ensured, the detector 242 is used for detecting a sample component separation belt, different separated sample collecting bottles 27 are driven to rotate under the rotation of the second rotating mechanism 22 to butt the butt-joint mechanism 28 for collecting different types of separated samples, and all required types of separated samples can be separated and collected at one time.
The first cavity 11 comprises an upper shell 111 with an opening at one end and an annular butt-joint ring 113, and an organ-shaped telescopic elastic body 112 is connected between the upper shell 111 and the annular butt-joint ring; the outer wall of the upper shell 111 is connected with an L-shaped guide rod 114.
The middle of the first cavity 11 contains a telescopic elastic body 112, which is beneficial to the up-and-down movement of the original sample collection bottle 13, so that the spiral mechanism 123 is placed in the sample collection bottle to be processed to stir the biochemical sample, and meanwhile, the disassembly, collection, cleaning and installation are not influenced; the L-shaped guide rod 114 connected to the outer wall of the upper housing 111 is used for guiding and stopping rotation of the sample collection bottle 13, and only moves linearly up and down.
The original sample collection bottle 13 is a shell with an opening at one end, and the opening side is in threaded connection with the butt-joint ring 113; the outer wall is provided with a guide groove 131 matched with the guide rod 114 along the axial direction; the center of the inner cavity is connected with a screw 132 connected with the first connecting shaft 122 through a thread pair.
The original sample collecting bottle 13 is detachably connected with the butt-joint ring 113, so that a biochemical sample to be treated can be collected conveniently; the guide slot 131 is matched with the guide rod 114 for guiding and stopping rotation, and only moves linearly up and down under the drive of the first connecting shaft 122 and the screw 132.
The first cavity 11 and the first rotating mechanism 12 are coaxial, and the contact surface is sealed; the second cavity 21 and the second rotating mechanism 22 are coaxial, and the contact surface is sealed; the upper surfaces of the first cavity 11 and the second cavity 21 are both adhered with metal with adsorbability. The coaxial arrangement stress is uniform, the structure is simple and reliable, the attached metal sheet is convenient to detach and recycle, the repeated utilization is realized, and the environmental pollution and the resource waste are reduced.
The separated sample collecting bottle 27 comprises a tubular bottle body 271 and a connecting part 272 arranged at the mouth part of the bottle body 271, and the processed biochemical sample is limited in the forming area of the two; the connecting part 272 is in threaded detachable connection with the sealing cover 274, and a sealing opening 273 is sealed on the surface of the connecting part 272 close to the sealing cover 274; the seal 273 is made of elastic material which is uniformly provided with three notches in the axial direction and can automatically return to be sealed; the separate sample collection vials 27, each time collecting a different separate sample, are of a different color, with the bottom of each body 271 and its corresponding cap 274 being marked with the same color.
The separated sample collecting bottle 27 comprises a tubular bottle body 271 and a threaded connecting part 272 for containing the processed biochemical sample, and the connecting part 272 comprises an elastic seal 273 which is provided with a middle cut and can automatically return to be sealed, so that the loss of internal vacuum generated by the shunting mechanism 24 is avoided; meanwhile, the sealing cover 274 can be detachably connected for protecting to prevent the sealing opening 273 from being touched by mistake to influence the quality of the separated sample, and the color marking is adopted for distinguishing to help quickly identify the type of the sample to be detected and prevent mixing the corresponding bottle body 271 and the sealing cover 274 to influence the detection effect.
The shunt mechanism 24 comprises a cross tube 241 and a resistance loop 245 arranged in the cross tube, wherein the cross tube 241 is a cross capillary, the closed end at the opposite side of the separated sample inflow port is provided with a detector 242, and the other two ends respectively correspond to the outlet of the buffer solution 26 and the mounting port of the shunt tube 243; an L-shaped return pipe 23 with a downward pipe orifice is arranged above the flow dividing mechanism 24 and is connected with the inner side wall of the second cavity 21.
The cross tube 241 is a cross capillary tube used for conveying different separation components after electrophoretic separation, a detector 242 used for detecting a separation zone of the sample components is arranged at the closed end on the opposite side of the separation sample inflow port, the sample components are accumulated under the action of a resistance loop 245, and the detection is sensitive; the other two ends respectively correspond to the buffer solution 26 discharge port and the shunt pipe 243 mounting port and are used for discharging separated samples; the shunt mechanism 24 and the L-shaped return pipe 23 connected with the inner side wall of the second cavity 21 can complete the recycling of the buffer solution 26 in the first cavity 11 and the second cavity 21 and reduce pollution.
The shunt pipe 243 is a venturi tube, a one-way valve 244 is arranged in the venturi tube, and the other end of the shunt pipe is connected with a hose 243 a; the resistance loop 245 comprises a resistance loop consisting of a first resistance 245a positioned at the outlet side of the buffer solution 26 and a second resistance 245b close to the shunt pipe 243; the first resistor 245a is larger than the second resistor 245 b.
The shunt pipe 243 is a venturi tube, the first resistor 245a of the resistor loop 245 is larger than the second resistor 245b, the moving direction of the separated sample is changed under the action of the resistor loop 245, the separated sample is enabled to continuously move downwards and is ejected out through the shunt pipe 243 of the venturi tube to form a negative pressure area, and the check valve 244 is used for controlling whether the separated sample flows out or not.
The docking mechanism 28 comprises a fixed seat 281 positioned on the bottom surface of the second cavity 21 and a tubular sleeve 282 hinged with the fixed seat 281; a spring elastic piece 283 is connected between the outer wall of the sleeve 282 and the bottom surface of the second cavity 21; the elastic piece 283 can adaptively adjust the inclination angle of the sleeve 282 and the bottom surface of the second cavity 21; the hose 243a passes through the bottom of the second chamber 21 and the sleeve 282 and is rotatably inserted into the seal 273 of the corresponding separated sample collection bottle 27 by the sleeve 282 for drainage.
The docking mechanism 28 can be inserted into the seal 273 of the corresponding bottle mouth under the action of the elastic member 283 to collect the separated sample, when the detector 242 detects that the collection of the separated sample is completed, the separated sample collecting bottle 27 is rotated and switched, the separated sample collecting bottle 27 pushes the docking mechanism 28 to incline upwards, the elastic member 283 is compressed until another separated sample collecting bottle 27 rotates to the right position, and the docking mechanism 28 is reinserted into the seal 273 of the corresponding bottle mouth under the action of the elastic member 283 to collect the injection.
An assembly for biochemical sample collection, using the above device for biochemical sample collection, further comprising a drive mechanism 3; the driving mechanism 3 is provided with a collecting and filtering mechanism 1 and a separating and purifying mechanism 2 which can be removed and are installed by magnetic adsorption or threads; a through drainage tube is horizontally connected between the first cavity 11 and the side wall of the second cavity 21 corresponding to the separated sample inlet and the return tube 23 of the cross tube 241; the driving mechanism 3 can drive the first connecting shaft 122 and the second connecting shaft 222 to rotate respectively by butting the first notch 121 and the second notch 221; the driving mechanism 3 can connect and electrify the first electrode 14 and the second electrode 25 to carry out electrophoretic separation.
A subassembly for biochemical sample is collected, including collecting filter mechanism 1, separation and purification mechanism 2 and actuating mechanism 3, collect filter mechanism 1 and separation and purification mechanism 2 and adopt magnetic force to adsorb or the replacement of being convenient for of threaded mounting on actuating mechanism 3, actuating mechanism 3 is reuse mechanism, helps reduce cost. A through drainage tube is horizontally connected between the first cavity 11 and the second cavity 21, and whether the first electrode 14 and the second electrode 25 are electrified or not is controlled to carry out electrophoretic separation on a sample; and provides power for the first connecting shaft 122 and the second connecting shaft 222.
The driving mechanism 3 comprises a shell 31, a driver 34, a first motor 32 and a second motor 33, wherein the driver 34, the first motor 32 and the second motor 33 are arranged on the bottom surface of an inner cavity of the shell in parallel, and the shaft ends of the first motor 32 and the second motor 33 are respectively connected with a first fork piece 321 and a second fork piece 331; the first fork piece 321, the second fork piece 331, the first notch 121 and the second notch 221 are in spline connection and are in annular limit fit butt joint driving; the mounting surfaces of the first motor 32 and the second motor 33 are respectively and coaxially provided with a first annular magnetic ring 35 and a second annular magnetic ring 36 which can perform magnetic adsorption corresponding to a motor shaft, and the lower surface of the corresponding shell 31 is respectively and coaxially provided with a first annular flange 311 and a second annular flange 312; the first flange 311 limits the first cavity 11, a first electrifying device 313 is arranged at the contact position of the lower surface of the shell 31 and the first cavity 11, the second flange 312 limits the second cavity 21, and a second electrifying device 314 is arranged at the contact position of the lower surface of the shell 31 and the second cavity 21.
After the first fork piece 321 and the second fork piece 331 of the driving mechanism 3 are in butt joint with the first notch 121 and the second notch 221, the driving is controlled by a first motor 32 and a second motor 33; the first flange 311 and the second flange 312 are used for quick and accurate positioning; the first electrifying device 313 and the second electrifying device 314 are used for conducting the first electrode 14 and the second electrode 25 to carry out electrophoretic separation; the magnetic adsorption is high in safety, not easy to fall off, and convenient and fast to remove.
Example 2:
when the device and the assembly for collecting biochemical samples are actually used, the driving mechanism 3 is firstly fixed, the first cover 111a and the second cover 111b which are arranged on the upper and lower hole positions on the side wall of the first cavity 11 for sealing and antifouling and the third cover 21a and the fourth cover 21b which are arranged on the upper and lower hole positions on the side wall of the second cavity 21 for sealing and leakage prevention are pulled out, the drainage tubes are connected with the corresponding hole positions, and the bottle body 271 of the separated sample collecting bottle 27 is put into the rotary table 223. And (3) adsorbing and suspending the butted collection filtering mechanism 1 and the separation and purification mechanism 2 at corresponding positions on the driving mechanism 3. The original sample collecting bottle 13 is screwed into the opening side of the cavity I11 to form a closed space after collecting a sample, when the rotating mechanism I12 rotates, under the guiding action of the guide rod 114, the connecting shaft I122 drives the screw rod 132 to move so as to drive the original sample collecting bottle 13 to move up and down, the buffer solution 26 in the cavity II 21 is transferred into the cavity I11 by utilizing the suction principle, meanwhile, the sample in the original sample collecting bottle 13 is stirred by the spiral mechanism 123 in the up-and-down movement process, the original sample collecting bottle 13 stays at the upper limit position after the stirring is completed, and precipitates and mixed liquid are separated by the diaphragm 124. The first conducting electrode 14 and the second conducting electrode 25 conduct electrophoresis separation on the mixed liquid, the components are separated due to differential migration, the movement direction of the components is changed through the resistance loop 245 in the shunt mechanism 24, the components are enabled to continuously move downwards, and the components are ejected out through the venturi pipe shunt 243 to form a negative pressure area. In the process of docking the corresponding separated sample collection bottle 27 by the docking mechanism 28, the treated biochemical sample is pumped into the separated sample collection bottle 27 by the shunt pipe 243 and the negative pressure generated constantly by the shunt pipe 243, the collected sample ejected by the shunt pipe 243 is a homogeneous flow and can be directly used for detection, the separated sample collection bottle 27 is simultaneously vacuum-filled, the detector 242 is used for detecting a sample component separation zone, different separated sample collection bottles 27 are driven to rotate to dock the docking mechanism 28 for collecting different types of separated samples under the rotation of the second rotating mechanism 22, all separated samples of required types can be separated and collected at one time, the check valve 244 is opened for collection after each transfer and alignment, and the check valve 244 is closed at other times to prevent liquid leakage. And after all the separated samples are collected, powering off, taking out the body 271 of the separated sample collecting bottle 27 and screwing the body 271 with the corresponding sealing cover 274 or directly screwing the body 274 with the corresponding sealing cover 274 after detection. Then the cleaning, collecting and filtering mechanism 1 and the separating and purifying mechanism 2 are dismantled for secondary use or can be directly replaced as a disposable consumable, and the driving mechanism 3 can be reused. The device can be used for high-efficiency high-quality stirring, filtering, separating, collecting and storing different types of biochemical samples.
Conventional techniques in the above embodiments are known to those skilled in the art, and therefore, will not be described in detail herein.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (10)
1. The device for collecting biochemical samples comprises a collecting and filtering mechanism (1) and a separating and purifying mechanism (2), and is characterized in that: the collecting and filtering mechanism (1) comprises:
the chamber I (11) is adjustable in height and provided with an opening and a built-in first electrode (14) capable of being electrified;
an intact sample collection vial (13) containing a biochemical sample to be treated, detachably connected to the open side of said first chamber (11); the rotating mechanism I (12) comprises a connecting shaft I (122) with two ends respectively connected with the notch I (121) and the screw mechanism (123) and is used for driving the original sample collecting bottle (13) to move up and down along the cavity I (11), and the cavity I (11) is divided into two areas by a filtering membrane (124) connected with the middle part of the connecting shaft I (122);
the separation and purification mechanism (2) comprises:
a second chamber (21) filled with a buffer solution (26) transferable into the first chamber (11) and a second electrode (25) with a polarity opposite to that of the first electrode (14);
a second rotating mechanism (22) which comprises a second connecting shaft (222) with two ends respectively connected with the second notch (221) and the turntable (223), wherein the turntable (223) is used for placing at least one removable separated sample collecting bottle (27);
a flow dividing mechanism (24) for separating and discharging the processed biochemical sample and the buffer solution (26);
and the docking mechanism (28) is used for docking the processed biochemical sample into the separated sample collecting bottle (27) by adaptively adjusting the inclination angle.
2. The device for biochemical sample collection according to claim 1, wherein: the first cavity (11) comprises an upper shell (111) with an opening at one end and an annular butt-joint ring (113), and the upper shell and the annular butt-joint ring are connected by a telescopic elastic body (112); the outer wall of the upper shell (111) is connected with an L-shaped guide rod (114).
3. The device for biochemical sample collection according to claim 1 or 2, wherein: the original sample collecting bottle (13) is a shell with an opening at one end, and the opening side of the shell is in threaded connection with the butt joint ring (113); the outer wall is provided with a guide groove (131) matched with the guide rod (114) along the axial direction; the center of the inner cavity is connected with a screw rod (132) which is connected with the first connecting shaft (122) through a thread pair.
4. The device for biochemical sample collection according to claim 1, wherein: the first cavity (11) and the first rotating mechanism (12) are coaxial, and the contact surface is sealed; the second cavity (21) and the second rotating mechanism (22) are coaxial, and the contact surface is sealed.
5. The device for biochemical sample collection according to claim 1, wherein: the separated sample collecting bottle (27) comprises a tubular bottle body (271) and a connecting part (272) arranged at the mouth part of the bottle body (271), and the processed biochemical sample is limited in the forming area of the tubular bottle body (271) and the connecting part; the connecting part (272) is in threaded detachable connection with the sealing cover (274), and a sealing opening (273) is sealed on the connecting part (272) close to the sealing cover (274); the seal (273) is made of elastic material with a middle cut and capable of automatically returning and sealing.
6. The device for biochemical sample collection according to claim 1, wherein: the flow dividing mechanism (24) comprises a cross pipe (241) and a resistance loop (245) arranged in the cross pipe, wherein the cross pipe (241) is a cross capillary, a detector (242) is arranged at the closed end of the opposite side of the separated sample inflow port, and the other two ends of the cross pipe respectively correspond to the outlet of the buffer solution (26) and the mounting port of the flow dividing pipe (243); and an L-shaped return pipe (23) with a downward pipe orifice is arranged above the flow distribution mechanism (24) and is connected with the inner side wall of the second cavity (21).
7. The device for biochemical sample collection according to claim 6, wherein: the shunt pipe (243) is a Venturi tube, a one-way valve (244) is arranged in the Venturi tube, and the other end of the shunt pipe is connected with a hose (243 a); the resistance loop (245) comprises a resistance loop formed by a first resistance (245a) positioned at the outlet side of the buffer solution (26) and a second resistance (245b) close to the shunt pipe (243); the first resistor (245a) is greater than the second resistor (245 b).
8. The device for biochemical sample collection according to claim 1 or 7, wherein: the butting mechanism (28) comprises a fixed seat (281) positioned on the bottom surface of the second cavity (21) and a tubular sleeve (282) hinged with the fixed seat (281); a spring elastic piece (283) is connected between the outer wall of the sleeve (282) and the bottom surface of the second cavity (21); the elastic piece (283) can adaptively adjust the inclination angle of the sleeve (282) and the bottom surface of the second cavity (21); the hose (243a) penetrates through the bottom of the second cavity (21) and the sleeve (282) and is inserted into a seal (273) of the corresponding separation sample collecting bottle (27) for drainage by rotating the sleeve (282).
9. The assembly for biochemical sample collection according to claim 1, wherein: use of a device for biochemical sample collection according to claims 1-8, further comprising a drive mechanism (3); the driving mechanism (3) is provided with a collecting and filtering mechanism (1) and a separating and purifying mechanism (2) which can be removed and are installed by magnetic adsorption or threads; a through drainage tube is horizontally connected between the first cavity (11) and the side wall of the second cavity (21) corresponding to the separation sample inflow port of the cross pipe (241) and the return pipe (23); the driving mechanism (3) can be butted with the first notch (121) and the second notch (221) to respectively drive the first connecting shaft (122) and the second connecting shaft (222) to rotate; the driving mechanism (3) can be communicated with the first electrode (14) and the second electrode (25) to carry out electrophoretic separation.
10. The assembly for biochemical sample collection of claim 9, wherein: the driving mechanism (3) comprises a shell (31), a driver (34), a first motor (32) and a second motor (33), wherein the driver (34), the first motor (32) and the second motor (33) are arranged on the bottom surface of an inner cavity of the shell in parallel, and shaft ends of the first motor (32) and the second motor (33) are respectively connected with a first fork piece (321) and a second fork piece (331); the first fork piece (321) and the second fork piece (331) are in annular limit fit with the first notch (121) and the second notch (221) and are in butt joint driving.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114130105A (en) * | 2021-09-09 | 2022-03-04 | 海南欣芯生物科技有限公司 | Purification device is used in tetrapeptide production with impurity separation structure |
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