CN220425387U - Pipette with sterilization function for cell culture - Google Patents
Pipette with sterilization function for cell culture Download PDFInfo
- Publication number
- CN220425387U CN220425387U CN202321717646.2U CN202321717646U CN220425387U CN 220425387 U CN220425387 U CN 220425387U CN 202321717646 U CN202321717646 U CN 202321717646U CN 220425387 U CN220425387 U CN 220425387U
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- pipe
- cell culture
- pipette
- outer side
- mounting
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 37
- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 23
- 238000009434 installation Methods 0.000 claims description 16
- 230000005540 biological transmission Effects 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 7
- 239000000853 adhesive Substances 0.000 claims description 4
- 230000001070 adhesive effect Effects 0.000 claims description 4
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 8
- 239000006143 cell culture medium Substances 0.000 abstract description 3
- 230000003993 interaction Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 230000009471 action Effects 0.000 description 5
- 239000000428 dust Substances 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004243 sweat Anatomy 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000003003 spiro group Chemical group 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of cell culture pipettes, and discloses a cell culture pipette with a sterilization function. According to the utility model, through the interaction cooperation of the filter net bar and the sterilizing lamp, the air in the gun body is replaced, and meanwhile, the air which is extracted and replaced is sterilized and decontaminated, so that the pollution of the replaced air to the inside of the gun body is avoided, and the pollution of the cell culture medium by the mixed bacteria is reduced, and the sterile quality of the subsequent pipetting operation is further ensured.
Description
Technical Field
The utility model is suitable for the technical field of cell culture pipettes, and particularly relates to a cell culture pipette with a sterilization function.
Background
Currently, cell culture is an important technology in the fields of biology and medicine, and aims to enable cells to grow and reproduce under in vitro conditions, but when cells are cultured, the cells need to be placed inside a culture dish filled with a culture medium, and when liquid culture media and corresponding cells are subjected to mobile treatment, corresponding pipettes are often required, and the pipettes used are also called pipette guns, and are an auxiliary tool for taking liquid from an original container into another container within a certain range, and are widely used in the fields of biology, chemistry and the like.
However, at present, before the pipette is used, when the pipette is not used and is kept stand, a large amount of external air is filled in the pipette, a large amount of bacterial impurities are easily bred in the accumulated air, and then before the pipette is used, the air in the pipette is required to be discharged and replaced, so that the contamination of cell liquid caused by the sundry bacteria in the air during the subsequent negative pressure pipetting is avoided, but when the pipette is replaced, no corresponding protection and sterilization mechanism exists outside the pipette, and further, once the dust and sundry bacteria are doped in the external air to be replaced, the air mixed with the sundry bacteria is still doped in the pipette after the air is discharged and replaced, so that the subsequent sterile environment required for transferring cells and liquid culture mediums is not ensured, and the subsequent cell culture quality is influenced. Therefore, we propose a pipette for cell culture with a sterilization function.
Disclosure of Invention
The utility model mainly aims to provide a pipette with a sterilization function for cell culture, which can effectively solve the problems in the background technology.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: the cell culture pipette with the sterilization function comprises a gun body, wherein exhaust pipes are fixedly arranged at two ends of the inner side of the gun body, a conveying pipe is fixedly arranged at the middle end of the bottom of the inner side of the gun body in a penetrating manner, and the side edge of the bottom of the exhaust pipe is fixedly communicated with the outer side of the conveying pipe; the surface of the outer side of the bottom of the gun body is fixedly provided with a fixed pipe, the conveying pipe is positioned in the fixed pipe, the outer side of one end, far away from the gun body, of the fixed pipe is screwed with a mounting pipe through threads, the top of the inner side wall of the mounting pipe is provided with threads matched with the outer side wall of the bottom of the gun body, the surfaces of the two ends of the bottom of the inner side of the mounting pipe are provided with mounting grooves, the number of the mounting grooves is four, and a sterilizing lamp tube is fixedly arranged between the inner sides of the two groups of mounting grooves; the filter screen plate is movably mounted at the top of the inner side of the mounting tube, the filter screen plate is positioned above the sterilizing lamp tube, and the bottom of the mounting tube is fixedly communicated with the air suction head.
By adopting the technical scheme, through the interaction cooperation of the filter net bar and the sterilizing lamp, the air which is extracted and replaced is sterilized and decontaminated when the air in the gun body is replaced, the air which is replaced is prevented from polluting the inside of the gun body and reducing the cell culture medium polluted by the miscellaneous bacteria, and the sterile quality of the follow-up pipetting operation is further ensured.
As an alternative scheme of this application technical scheme, the equal vertical fixed mounting in filter screen board top both ends surface has the transfer line, and transfer line slidable mounting is inside the installation pipe, limit baffle has been placed in installation pipe top surface activity laminating, fixed pipe activity grafting is inside limit baffle, and the transfer line top is connected with limit baffle bottom surface stationary phase. By adopting the technical scheme, the filter screen plate is connected with the limit baffle through the transmission rod, so that an operator can conveniently disassemble and assemble the filter screen plate.
As an alternative scheme of this application technical scheme, piston rod top fixed surface installs the hand push board, and hand push board is located rifle outside top, hand push board outside fixed surface bonding has cup jointed sweat-absorbing sleeve, negative pressure groove has been seted up to the internal middle-end of rifle, and the conveyer pipe top is linked together with negative pressure groove bottom, the vertical slidable mounting that runs through in rifle body top middle part has the piston rod, and piston rod bottom movable mounting in negative pressure inslot portion, piston rod bottom fixed surface installs the piston plate, and piston plate slidable mounting in negative pressure inslot portion. By adopting the technical scheme, the negative pressure extraction treatment of external liquid is realized through the cooperation of the piston plate and the piston rod.
As an alternative of the technical scheme of the application, the bottom of the inner side of the negative pressure groove is sleeved with a pushing spring in a sliding manner, the top of the pushing spring is movably attached to the bottom surface of the piston plate, and the outer side surface of the piston plate is sleeved with a sealing gasket in an adhesive manner. By adopting the technical scheme, the abutting and restoring treatment of the piston plate is realized through the structural action of the pushing spring.
As an alternative scheme of the technical scheme of the application, the sliding groove is vertically formed in two ends of the inner side of the installation pipe, and the transmission rod penetrates through the sliding groove to be installed inside the sliding groove. By adopting the technical scheme, the filter screen plate is conveniently disassembled and assembled under the guiding and sliding action of the sliding groove on the transmission rod.
As an alternative scheme of this application technical scheme, rifle outside top surface fixed mounting has holds the piece, conveyer pipe bottom outside surface fixation cup joints the stopper. By adopting the technical scheme, the mounting stability of the liquid suction pipe during sleeving is ensured through the limiting block.
As an alternative scheme of this application technical scheme, every exhaust pipe top outside surface all fixed mounting has the aspiration pump, every the exhaust pipe top is screwed up through the screw thread and is had sealed screw thread lid, every sealed screw thread lid inboard top surface fixed bonding has sealed the pad, and sealed pad movable mounting is inside the exhaust pipe. By adopting the technical scheme, through the structural effect of the sealing thread cover and the sealing gasket, the inside of the exhaust pipe is ensured not to be polluted by external air when pipetting is carried out.
Compared with the prior art, the utility model has the following beneficial effects:
1. according to the pipette with the sterilization function for cell culture, the corresponding sterilization lamp tubes are inserted between the mounting grooves in the mounting tubes, and the mounting tubes are screwed below the fixed tubes by communicating the exhaust tubes with the conveying tubes, so that the exhaust tubes, the conveying tubes and the mounting tubes can form corresponding communication states. The external air can be pumped and conveyed to the inside of the installation pipe through the air suction head under the operation action of the air suction pump, and then the external air flows and irradiates and sterilizes the installation pipe through the sterilizing lamp tube in the process of flowing through the conveying pipe to the inside of the exhaust pipe, so that the mixed bacteria doped in the pumped and flowing air are sterilized. Then flows through the filter screen plate, combines the filtering action of the filter screen plate to filter and remove impurity from doped dust impurities in the air, and when the air in the gun body is replaced, the air which is extracted and replaced is sterilized and removed, so that the air which is replaced still causes pollution in the gun body to reduce the cell culture medium polluted by the impurity bacteria, and further the sterile quality of the follow-up pipetting operation is ensured.
2. According to the cell culture pipette with the sterilization function, the bottom of the limit baffle is connected with the top of the filter screen plate through the transmission rod, and the filter screen plate can be moved out of the installation tube along with the vertical movement of the limit baffle under the guiding sliding action of the transmission rod by combining the sliding groove, so that an operator can conveniently clean dust impurities accumulated at the bottom of the filter screen plate, and the quality of filtering and impurity removal of air in the long-time use process of the filter screen plate is ensured.
Drawings
FIG. 1 is a schematic view showing the overall perspective structure of a pipette for cell culture with a sterilization function according to the present utility model;
FIG. 2 is a schematic diagram showing the whole cross-sectional elevation structure of a pipette for cell culture with sterilization function according to the present utility model;
FIG. 3 is a schematic top view of a sectional structure of a mounting tube of a pipette for cell culture with a sterilization function according to the present utility model.
Reference numerals: 1. a gun body; 11. a delivery tube; 12. a piston rod; 13. a piston plate; 14. a gasket sleeve; 15. a negative pressure tank; 16. a fixed tube; 17. holding the block; 2. an exhaust pipe; 21. an air extracting pump; 22. a mounting groove; 23. sterilizing the lamp tube; 24. a filter screen plate; 25. a limit baffle; 26. a transmission rod; 27. a sliding groove; 28. an air suction head; 29. installing a pipe; 3. sealing the screw cap; 31. a sealing gasket; 4. a pushing spring; 41. a hand pushing plate; 42. a sweat absorbing sleeve.
Detailed Description
As shown in fig. 1-3, a pipette for cell culture with sterilization function, the two end surfaces of the top of the filter screen plate 24 are vertically and fixedly provided with a transmission rod 26, the transmission rod 26 is slidably mounted in a mounting tube 29, the top surface of the mounting tube 29 is movably attached to the top surface of the mounting tube, a limit baffle 25 is placed, a fixed tube 16 is movably inserted in the limit baffle 25, and the top of the transmission rod 26 is fixedly connected with the bottom surface of the limit baffle 25.
In this technical scheme (through the illustration of fig. 1, 2 and 3), the equal vertical fixed mounting in filter screen plate 24 top both ends surface has transfer line 26, and transfer line 26 slidable mounting is inside installation pipe 29, and limit baffle 25 has been placed in the laminating of installation pipe 29 top surface activity, and fixed pipe 16 activity is pegged graft inside limit baffle 25, and transfer line 26 top and limit baffle 25 bottom surface stationary phase are connected.
In this technical scheme (through the illustration of fig. 1, 2 and 3), every blast pipe 2 top outside surface all fixed mounting has aspiration pump 21, and every blast pipe 2 top has sealed screw cap 3 through the screw thread spiro union, and every sealed screw cap 3 inboard top surface fixed bonding has sealed pad 31, and sealed pad 31 movable mounting is inside blast pipe 2.
In one embodiment, the fixing tube 16 is fixedly installed on the outer side surface of the bottom of the gun body 1, and the conveying tube 11 is located inside the fixing tube 16. The outside of one end of the fixed pipe 16, which is far away from the gun body 1, is screwed with a mounting pipe 29 through threads, and threads matched with the outer side wall of the bottom of the gun body 1 are formed at the top of the inner side wall of the mounting pipe 29. The mounting tube 29 is inboard bottom both ends surface has all offered mounting groove 22, and the quantity of mounting groove 22 is four sets of, and every two sets of mounting groove 22 inboards of level are fixed mounting has the sterilizing lamp tube 23, and mounting tube 29 inboard top movable mounting has the filter screen plate 24, and the filter screen plate 24 is located sterilizing lamp tube 23 top, and mounting tube 29 bottom fixedly communicates has the air suction head 28.
The top surface of the outer side of the gun body 1 is fixedly provided with a hand holding block 17, and the outer side surface of the bottom of the conveying pipe 11 is fixedly sleeved with a limiting block 43. The top surface of the piston rod 12 is fixedly provided with a hand push plate 41, the hand push plate 41 is positioned at the top of the outer side of the gun body 1, and the outer side surface of the hand push plate 41 is fixedly and fixedly connected with a sweat absorbing sleeve 42 in an adhesive manner. The negative pressure groove 15 has been seted up to the inside middle-end of rifle body 1, and conveyer pipe 11 top is linked together with negative pressure groove 15 bottom, and the vertical sliding mounting that runs through in rifle body 1 top middle part has piston rod 12, and piston rod 12 bottom movable mounting is in negative pressure groove 15 inside, and piston rod 12 bottom surface fixed mounting has piston plate 13, and piston plate 13 sliding mounting is in negative pressure groove 15 inside.
Preferably, the bottom of the inner side of the negative pressure groove 15 is in sliding sleeve connection with the pushing spring 4, the top of the pushing spring 4 is movably attached to the bottom surface of the piston plate 13, and the outer side surface of the piston plate 13 is in adhesive sleeve connection with the sealing gasket sleeve 14.
When the gun body 1 which stands for a period of time is required to be subjected to pipetting operation in operation, the limit baffle 25 and the filter screen plate 24 at the bottom of the limit baffle are pushed to be placed in the mounting tube 29, so that the transmission rod 26 is slidably inserted into the sliding groove 27, the top of the mounting tube 29 is sleeved outside the bottom end of the fixed tube 16, the mounting tube 29 is screwed to be screwed and fixed on the outer side surface of the bottom of the fixed tube 16, and the sterilizing lamp tube 23 is opened by synchronizing an external control switch. After the screw cap 3 is screwed outward so that the gasket 31 at the bottom thereof can be removed from the top of the exhaust pipe 2, the two sets of suction pumps 21 are turned on by an external control switch to operate and generate negative pressure inside the exhaust pipe 2. Then, the outside air is sent to the inside of the mounting tube 29, the delivery tube 11 and the exhaust tube 2 through the air suction head 28, the air in the delivery tube 11 and the gun body 1 is discharged and replaced, and the air is irradiated and sterilized through the sterilizing lamp tube 23 in the air circulation process, and the mixed bacteria doped in the air which is extracted and flowed are sterilized, and then the air flows through the filter screen 24. The air is filtered and decontaminated by combining the filtering effect of the filter screen plate 24, the air in the gun body 1 is replaced, meanwhile, the air which is extracted and replaced is sterilized and decontaminated, after the air is filtered and sterilized and replaced, the sterilizing lamp tube 23 and the air pump 21 are closed, the mounting tube 29 is screwed out from the bottom of the fixed tube 16, the corresponding liquid suction head is sleeved on the outer wall of the conveying tube 11, the piston rod 12 is pressed to drive the piston plate 13 to move in the negative pressure groove 15 to generate corresponding negative pressure in the conveying tube 11, the liquid suction head is inserted into the cell culture dish, the piston rod 12 is loosened, and when the piston plate 13 is pushed by the pushing spring 4 to reset, the cell liquid is extracted, and the corresponding liquid removal operation is completed.
The foregoing descriptions of specific exemplary embodiments of the present utility model are presented for purposes of illustration and description. It is not intended to limit the utility model to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the utility model and its practical application to thereby enable one skilled in the art to make and utilize the utility model in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the utility model be defined by the claims and their equivalents.
Claims (8)
1. Cell culture pipette with sterilization function, including rifle body (1), its characterized in that: the two ends of the inner side of the gun body (1) are fixedly provided with exhaust pipes (2), the middle end of the bottom of the inner side of the gun body (1) is fixedly provided with a conveying pipe (11) in a penetrating manner, and the side edge of the bottom of the exhaust pipe (2) is fixedly communicated with the outer side of the conveying pipe (11);
the automatic sterilizing device is characterized in that a fixed pipe (16) is fixedly arranged on the outer side surface of the bottom of the gun body (1), a conveying pipe (11) is positioned inside the fixed pipe (16), an installation pipe (29) is screwed on the outer side of one end, far away from the gun body (1), of the outer side of the fixed pipe (16), threads matched with the outer side wall of the bottom of the gun body (1) are formed in the top of the inner side wall of the installation pipe (29), installation grooves (22) are formed in the surfaces of two ends of the inner side bottom of the installation pipe (29), the number of the installation grooves (22) is four, and sterilizing lamp tubes (23) are fixedly arranged between the inner sides of the two groups of the installation grooves (22) in each horizontal mode;
the filter screen plate (24) is movably mounted at the top of the inner side of the mounting tube (29), the filter screen plate (24) is positioned above the sterilizing lamp tube (23), and the bottom of the mounting tube (29) is fixedly communicated with the air suction head (28).
2. The pipette for cell culture with sterilization function according to claim 1, wherein: the filter screen plate (24) is characterized in that transmission rods (26) are vertically and fixedly installed on the surfaces of two ends of the top of the filter screen plate, the transmission rods (26) are slidably installed inside installation pipes (29), limit baffles (25) are movably attached to the surfaces of the tops of the installation pipes (29), the fixed pipes (16) are movably inserted into the limit baffles (25), and the tops of the transmission rods (26) are fixedly connected with the surfaces of the bottoms of the limit baffles (25).
3. The pipette for cell culture with sterilization function according to claim 1, wherein: negative pressure groove (15) has been seted up to the inside middle-end of rifle body (1), and conveyer pipe (11) top is linked together with negative pressure groove (15) bottom, the vertical slidable mounting that runs through in rifle body (1) top middle part has piston rod (12), and piston rod (12) bottom movable mounting in negative pressure groove (15) inside, piston rod (12) bottom fixed surface installs piston plate (13), and piston plate (13) slidable mounting is inside negative pressure groove (15).
4. A pipette for cell culture having a sterilization function according to claim 3, wherein: the hand push plate (41) is fixedly arranged on the top surface of the piston rod (12), the hand push plate (41) is positioned at the top of the outer side of the gun body (1), and the sweat-absorbing sleeve (42) is fixedly bonded and sleeved on the surface of the outer side of the hand push plate (41).
5. A pipette for cell culture having a sterilization function according to claim 3, wherein: the bottom of the inner side of the negative pressure groove (15) is in sliding sleeve joint with a pushing spring (4), the top of the pushing spring (4) is movably attached to the bottom surface of the piston plate (13), and the outer side surface of the piston plate (13) is in adhesive sleeve joint with a sealing gasket sleeve (14).
6. The pipette for cell culture with sterilization function according to claim 1, wherein: the two ends of the inner side of the mounting pipe (29) are vertically provided with sliding grooves (27), and the transmission rod (26) penetrates through the sliding grooves (27) to be mounted inside.
7. The pipette for cell culture with sterilization function according to claim 1, wherein: the gun is characterized in that a hand holding block (17) is fixedly arranged on the surface of the top of the outer side of the gun body (1), and a limiting block (43) is fixedly sleeved on the surface of the outer side of the bottom of the conveying pipe (11).
8. The pipette for cell culture with sterilization function according to claim 1, wherein: every exhaust pipe (2) top outside surface all fixed mounting has aspiration pump (21), every exhaust pipe (2) top is screwed up through the screw thread and is had sealed screw thread lid (3), every sealed screw thread lid (3) inboard top surface fixed bonding has sealed pad (31), and sealed pad (31) movable mounting is inside exhaust pipe (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321717646.2U CN220425387U (en) | 2023-07-03 | 2023-07-03 | Pipette with sterilization function for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321717646.2U CN220425387U (en) | 2023-07-03 | 2023-07-03 | Pipette with sterilization function for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220425387U true CN220425387U (en) | 2024-02-02 |
Family
ID=89696521
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321717646.2U Active CN220425387U (en) | 2023-07-03 | 2023-07-03 | Pipette with sterilization function for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220425387U (en) |
-
2023
- 2023-07-03 CN CN202321717646.2U patent/CN220425387U/en active Active
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