CN111560313A - Detection kit for enterobacter microorganisms and detection method thereof - Google Patents

Detection kit for enterobacter microorganisms and detection method thereof Download PDF

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Publication number
CN111560313A
CN111560313A CN202010406306.2A CN202010406306A CN111560313A CN 111560313 A CN111560313 A CN 111560313A CN 202010406306 A CN202010406306 A CN 202010406306A CN 111560313 A CN111560313 A CN 111560313A
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CN
China
Prior art keywords
box body
detection
detection kit
reagent box
reagent
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Pending
Application number
CN202010406306.2A
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Chinese (zh)
Inventor
罗海燕
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Baorui Shanghai Biotechnology Co ltd
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Baorui Shanghai Biotechnology Co ltd
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Priority to CN202010406306.2A priority Critical patent/CN111560313A/en
Publication of CN111560313A publication Critical patent/CN111560313A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D39/00Closures arranged within necks or pouring openings or in discharge apertures, e.g. stoppers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D43/00Lids or covers for rigid or semi-rigid containers
    • B65D43/14Non-removable lids or covers
    • B65D43/16Non-removable lids or covers hinged for upward or downward movement
    • B65D43/163Non-removable lids or covers hinged for upward or downward movement the container and the lid being made separately
    • B65D43/164Non-removable lids or covers hinged for upward or downward movement the container and the lid being made separately and connected by interfitting hinge elements integrally with the container and the lid formed respectively
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a detection kit for enterobacter microorganisms, which belongs to the technical field of enterobacter detection kits, and specifically relates to a detection kit for enterobacter microorganisms, which comprises a box body and a box cover, wherein the box cover is connected to the upper surface of the box body through a hinge, unit detection kits and labels are uniformly arranged in the box body, the unit detection kits and the labels are in one-to-one correspondence, and the detection method for the detection kit for the enterobacter microorganisms comprises the following specific steps: s1: taking down the corresponding unit detection kit at the corresponding label according to the type of the intestinal bacteria to be detected; s2: injecting the intestinal bacterium RNA to be detected into the reagent box body through the filling port and the rubber plug; s3: the power supply circuit of light is closed to supply battery through control switch, lights after the light circular telegram, through the mating reaction of one-to-one between label, the unit detect reagent box, can find required unit detect reagent box fast, reduces the pollution of external bacterium, guarantees the degree of accuracy that detects.

Description

Detection kit for enterobacter microorganisms and detection method thereof
Technical Field
The invention relates to the technical field of an enterobacteria detection kit, in particular to a detection kit for enterobacteria microorganisms and a detection method thereof.
Background
Due to the change of living habits, the incidence of intestinal diseases of China is increased year by year, the earlier intestinal diseases are discovered, the easier the intestinal diseases are to be treated, and the early diagnosis is very important and urgent. The existing invasive examination requires complete and thorough intestinal tract preparation, and has inconvenience of privacy exposure, sedation and the like, so that the invasive examination is difficult to be widely accepted by people. With the progress of medicine, the detection by adopting a gene kit is more and more extensive.
The intestinal microorganisms refer to a large number of microorganisms existing in the intestinal tract of animals, and the group of microorganisms depends on the intestinal life of the animals and simultaneously helps the hosts to complete various physiological and biochemical functions.Intestinal tractNot only is it an important site for human digestion and absorption, but it is also the largest immune organ and plays an extremely important role in maintaining normal immune defense functions. The human intestinal tract provides a good inhabitation environment for microorganisms and has a metabolism function which is not possessed by the human body.
As the most huge and complex microecosystem of human body, the intestinal microorganisms and the metabolites thereof can not only regulate the health of human body, but also play an important bridge role between diet and host.
The detection kit is used for containing a box for detecting chemical reagents such as chemical components, drug residues, virus types and the like. Is generally used by hospitals and pharmaceutical enterprises.
When the existing detection kit is used, the storage, observation and taking out of a detection reagent are not convenient enough.
Disclosure of Invention
The invention aims to provide a detection kit for enterobacter microorganisms and a detection method thereof, and aims to solve the problem that the existing detection kit proposed in the background technology is inconvenient to store, observe and take out detection reagents when in use.
In order to achieve the purpose, the invention provides the following technical scheme: a detection kit for enterobacter microorganisms comprises a box body and a box cover, wherein the box cover is connected to the upper surface of the box body through hinges, unit detection kits and labels are uniformly arranged in the box body, and the unit detection kits and the labels are in one-to-one correspondence;
unit detect reagent box includes top cap and reagent box body, the top cap spiro union is at the upside of reagent box body, top one side of top cap is provided with the filler, circular logical groove has been seted up at the body middle part of top cap, and the embedding of circular logical inslot has perspective mirror, the inside downside embedding of filler has the rubber buffer, gene detect reagent is deposited to the inside of reagent box body.
Preferably, the upper surface of the box body is provided with a clamping groove, and the box cover is clamped in the clamping groove.
Preferably, the outer wall of the reagent box body is provided with an external thread ring, and an internal thread hole matched with the external thread ring is formed in the corresponding position inside the box body.
Preferably, the kit body is a transparent kit body.
Preferably, the bottom of reagent box body is provided with and holds the chamber, the inside that holds the chamber is provided with the light, the bottom that holds the chamber is provided with the bottom suspension fagging, be provided with power supply battery and control switch in the bottom suspension fagging, electric connection between light, power supply battery and the control switch, the bottom of bottom suspension fagging is connected with supporting spring.
Preferably, the bottom of the filling opening is provided with a conical plate, the conical plate is hollow and funnel-shaped, and the conical plate and the filling opening are coaxially arranged.
The detection method of the detection kit for the enterobacteria microorganisms comprises the following specific steps:
s1: according to the type of the intestinal bacteria to be detected, taking down the corresponding unit detection kit at the corresponding label, wherein the unit detection kit is taken down from the box body in a spiral mode;
s2: injecting the enterobacteria RNA to be detected into the reagent box body through the filling port and the rubber plug so that the gene detection reagent in the reagent box body reacts on the detection of the enterobacteria RNA;
s3: the power supply circuit of the illuminating lamp is closed by the control switch, the illuminating lamp is lightened after being electrified, and an experimenter observes the reaction in the reagent box body through the perspective lens to judge a detection result.
Compared with the prior art, the invention has the beneficial effects that:
1) through the arrangement of the filling port, the steps of opening and closing the reagent box body are reduced, the pollution of external bacteria is reduced, and the detection accuracy is ensured;
2) through the arrangement of the rubber plug and the conical plate, the reagent in the device can be prevented from flowing out;
3) through the one-to-one matching action between the label and the unit detection kit, the required unit detection kit can be quickly found, and the efficiency is improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic top view of the case according to the present invention;
FIG. 3 is a schematic view of the structure of the unit detection kit of the present invention;
FIG. 4 is a schematic sectional view of the unit detection kit of the present invention.
In the figure: the kit comprises a box body 1, a label 2, a 3-unit detection kit, a 31 reagent box body, a 32 top cover, a 33 perspective mirror, a 34 filling port, a 35 rubber plug, a 36 accommodating cavity, a 37 illuminating lamp, a 38 lower supporting plate, a 39 power supply battery, a 310 control switch, a 311 supporting spring, a 312 external thread ring and a 4 box cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example (b):
referring to fig. 1-4, the present invention provides a technical solution: a detection kit for enterobacter microorganisms comprises a box body 1 and a box cover 4, wherein the box cover 4 is connected to the upper surface of the box body 1 through hinges, unit detection kits 3 and labels 2 are uniformly arranged in the box body 1, and the unit detection kits 3 and the labels 2 are in one-to-one correspondence;
unit detect reagent box 3 includes top cap 32 and reagent box body 31, the top cap 32 spiro union is at the upside of reagent box body 31, top end one side of top cap 32 is provided with filling opening 34, circular logical groove has been seted up at the body middle part of top cap 32, and the embedding of circular logical inslot has perspective mirror 33, the inside downside embedding of filling opening 34 has rubber buffer 35, gene detect reagent is deposited to the inside of reagent box body 31.
Preferably, the upper surface of the box body 1 is provided with a clamping groove, and the box cover 4 is clamped in the clamping groove.
Preferably, an external thread ring 312 is arranged on the outer wall of the reagent box body 31, and an internal thread hole matched with the external thread ring 312 is formed in a corresponding position inside the box body 1.
Preferably, the reagent box body 31 is made of a transparent material.
Preferably, the bottom of the reagent box body 31 is provided with an accommodating cavity 36, an illuminating lamp 37 is arranged inside the accommodating cavity 36, the bottom of the accommodating cavity 36 is provided with a lower supporting plate 38, a power supply battery 39 and a control switch 310 are arranged on the lower supporting plate 38, the illuminating lamp 37, the power supply battery 39 and the control switch 310 are electrically connected, and the bottom of the lower supporting plate 38 is connected with a supporting spring 311.
Preferably, a conical plate is arranged at the bottom of the filling opening 34, the conical plate is in a hollow funnel shape, and the conical plate and the filling opening 34 are coaxially arranged.
A detection method of a detection kit for enterobacter microorganisms takes the detection of coliform groups as an example, and the detection method of the detection kit for the enterobacter microorganisms comprises the following specific steps:
s1: according to the type of the intestinal bacteria to be detected, the corresponding unit detection kit 3 is taken down from the corresponding label 2, and the unit detection kit 3 is taken down from the box body 1 in a spiral mode;
s2: injecting the intestinal bacterium RNA to be detected into the reagent box body 31 through the filling port 34 and the rubber plug 35, so that the gene detection reagent in the reagent box body 31 can perform detection reaction on the intestinal bacterium RNA;
the enterobacteria RNA to be detected is collected, and only patients with stage I or II in clinical stage in the rectal disease are selected for obtaining early diagnosis information, and III or IV are excluded. Alternatively, the control group was prepared from cases with other detection modalities (e.g., enteroscopy).
The gene detection reagent is composed of a conventional reagent including Bst RNA polymerase, reaction buffer solution, dNTP, magnesium sulfate, a specific primer group, betaine and sterile double distilled water, a fluorescent color developing agent and a detection tube with a sterilization filter paper sheet attached to an inner cover;
the specific primer group comprises outer primers F3 and B3, inner primers FIP and BIP, and the nucleotide sequences are as follows:
F3:5’-AGCTGGTCTGAGAGGATGAC-3’
B3:5’-CGGGTAACGTCAATGAGCAA-3’
FIP:5’-GCGCCCATTGTGCAATATTCCCTTTTCCACACTGGAACTGAGACAC-3’
BIP:5’-AAGCCTGATGCAGCCATGCCTTTTTTCCTCCCCGCTGAAAGT-3’。
s3: the power supply circuit of the lighting lamp 37 is switched on by the power supply battery 39 through the control switch 310, the lighting lamp 37 is lighted after being electrified, and an experimenter observes the reaction in the reagent box body 31 through the perspective mirror 33 to judge the detection result.
While there have been shown and described the fundamental principles and essential features of the invention and advantages thereof, it will be apparent to those skilled in the art that the invention is not limited to the details of the foregoing exemplary embodiments, but is capable of other specific forms without departing from the spirit or essential characteristics thereof; the present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. The utility model provides a detect reagent box for enterobacter microorganism, includes box body (1) and lid (4), its characterized in that: the box cover (4) is connected to the upper surface of the box body (1) through a hinge, unit detection kits (3) and labels (2) are uniformly arranged in the box body (1), and the unit detection kits (3) correspond to the labels (2) one by one;
unit detect reagent box (3) include top cap (32) and reagent box body (31), top cap (32) spiro union is at the upside of reagent box body (31), top one side of top cap (32) is provided with filling opening (34), circular logical groove has been seted up at the body middle part of top cap (32), and the embedding of circular logical inslot has perspective mirror (33), the inside downside embedding of filling opening (34) has rubber buffer (35), gene detect reagent is deposited to the inside of reagent box body (31).
2. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the upper surface of box body (1) is provided with the draw-in groove, lid (4) joint is in the draw-in groove.
3. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: an external thread ring (312) is arranged on the outer wall of the reagent box body (31), and an internal thread hole matched with the external thread ring (312) is formed in the corresponding position in the box body (1).
4. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the reagent box body (31) is made of transparent materials.
5. The detection kit for an enteric bacterium microorganism according to claim 4, characterized in that: the bottom of reagent box body (31) is provided with holds chamber (36), the inside that holds chamber (36) is provided with light (37), the bottom that holds chamber (36) is provided with bottom suspension fagging (38), be provided with on bottom suspension fagging (38) power supply battery (39) and control switch (310), electric connection between light (37), power supply battery (39) and the control switch (310), the bottom of bottom suspension fagging (38) is connected with supporting spring (311).
6. The detection kit for an enterobacteria microorganism according to claim 1, characterized in that: the bottom of filling port (34) is provided with the conical plate, and the conical plate is cavity hourglass hopper-shaped, conical plate and filling port (34) coaxial setting.
7. A method of detecting the detection kit for enteric bacteria microorganism as set forth in any one of claims 1 to 6, characterized in that: the detection method of the detection kit for the enterobacteria microorganisms comprises the following specific steps:
s1: according to the type of the intestinal bacteria to be detected, the corresponding unit detection kit (3) is taken down from the corresponding label (2), and the unit detection kit (3) is taken down from the box body (1) in a spiral mode;
s2: injecting the intestinal bacterium RNA to be detected into the reagent box body (31) through the filling port (34) and the rubber plug (35) so that the gene detection reagent in the reagent box body (31) can perform detection reaction on the intestinal bacterium RNA;
s3: the power supply circuit of the illuminating lamp (37) is closed by the control switch (310), the illuminating lamp (37) is lightened after being electrified, and an experimenter observes the reaction of the transparent mirror (33) in the reagent box body (31) to judge the detection result.
CN202010406306.2A 2020-05-14 2020-05-14 Detection kit for enterobacter microorganisms and detection method thereof Pending CN111560313A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010406306.2A CN111560313A (en) 2020-05-14 2020-05-14 Detection kit for enterobacter microorganisms and detection method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010406306.2A CN111560313A (en) 2020-05-14 2020-05-14 Detection kit for enterobacter microorganisms and detection method thereof

Publications (1)

Publication Number Publication Date
CN111560313A true CN111560313A (en) 2020-08-21

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586420A (en) * 2011-12-27 2012-07-18 盛司潼 Method and kit for assaying breast cancer susceptibility genes
US20140024543A1 (en) * 2012-07-23 2014-01-23 Pall Corporation Compositions for detecting foodstuff spoilage microorganisms
CN109055199A (en) * 2018-08-17 2018-12-21 苏州市苏测检测技术有限公司 A kind of food microorganism assay kit
CN109136080A (en) * 2018-09-25 2019-01-04 新乡医学院 A kind of detection kit and its application for the early diagnosis of straight colon cancer
CN109374888A (en) * 2018-11-21 2019-02-22 任宇 A kind of kit for cell detection
WO2019094973A1 (en) * 2017-11-13 2019-05-16 Life Technologies Corporation Compositions, methods and kits for urinary tract microorganism detection
CN209198473U (en) * 2018-12-26 2019-08-02 王晓东 A kind of microalbumin detection kit

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586420A (en) * 2011-12-27 2012-07-18 盛司潼 Method and kit for assaying breast cancer susceptibility genes
US20140024543A1 (en) * 2012-07-23 2014-01-23 Pall Corporation Compositions for detecting foodstuff spoilage microorganisms
WO2019094973A1 (en) * 2017-11-13 2019-05-16 Life Technologies Corporation Compositions, methods and kits for urinary tract microorganism detection
CN109055199A (en) * 2018-08-17 2018-12-21 苏州市苏测检测技术有限公司 A kind of food microorganism assay kit
CN109136080A (en) * 2018-09-25 2019-01-04 新乡医学院 A kind of detection kit and its application for the early diagnosis of straight colon cancer
CN109374888A (en) * 2018-11-21 2019-02-22 任宇 A kind of kit for cell detection
CN209198473U (en) * 2018-12-26 2019-08-02 王晓东 A kind of microalbumin detection kit

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Application publication date: 20200821